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2 results on '"MESH: Epithelial Cells / enzymology"'

1. Deimination, Intermediate Filaments and Associated Proteins

Author

Briot, Julie, Simon, Michel, Méchin, Marie-Claire, Unité différenciation épidermique et auto-immunité rhumatoïde (UDEAR), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), and CARBILLET, Véronique

Subjects
filaggrin, MESH: Neoplasms / immunology, Intermediate Filaments, Review, Filaggrin Proteins, lcsh:Chemistry, Arthritis, Rheumatoid, Intermediate Filament Proteins, MESH: Arthritis, Rheumatoid / genetics, MESH: Arthritis, Rheumatoid / immunology, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Neoplasms, MESH: Arthritis, Rheumatoid / pathology, peptidylarginine deiminase, lcsh:QH301-705.5, keratin, Neurons, MESH: Intermediate Filaments / ultrastructure, MESH: Protein Multimerization, MESH: Intermediate Filaments / enzymology, Cell Differentiation, Neurodegenerative Diseases, cytoskeleton, MESH: Protein Processing, Post-Translational, MESH: Protein-Arginine Deiminases / metabolism, MESH: Neoplasms / pathology, MESH: Multiple Sclerosis / enzymology, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, MESH: Protein-Arginine Deiminases / genetics, MESH: Neurodegenerative Diseases / genetics, MESH: Intermediate Filament Proteins / metabolism, MESH: Multiple Sclerosis / genetics, MESH: Intermediate Filament Proteins / genetics, MESH: Cell Differentiation, Multiple Sclerosis, citrullination, MESH: Neurodegenerative Diseases / immunology, MESH: Multiple Sclerosis / immunology, MESH: Arthritis, Rheumatoid / enzymology, MESH: Neurodegenerative Diseases / pathology, MESH: Epithelial Cells / pathology, MESH: Epithelial Cells / enzymology, Humans, MESH: Mesenchymal Stem Cells / enzymology, MESH: Mesenchymal Stem Cells / pathology, MESH: Multiple Sclerosis / pathology, MESH: Neurons / enzymology, MESH: Humans, MESH: Proteolysis Solubility, Epithelial Cells, Mesenchymal Stem Cells, MESH: Neurons / pathology, MESH: Neoplasms / enzymology, MESH: Neurodegenerative Diseases / enzymology, lcsh:Biology (General), lcsh:QD1-999, Solubility, post-translational modification, MESH: Protein-Arginine Deiminases / chemistry, MESH: Neoplasms / genetics, Proteolysis, Protein-Arginine Deiminases, MESH: Citrullination, Protein Multimerization, MESH: Intermediate Filament Proteins / chemistry, Protein Processing, Post-Translational
Abstract

International audience; Deimination (or citrullination) is a post-translational modification catalyzed by a calcium-dependent enzyme family of five peptidylarginine deiminases (PADs). Deimination is involved in physiological processes (cell differentiation, embryogenesis, innate and adaptive immunity, etc.) and in autoimmune diseases (rheumatoid arthritis, multiple sclerosis and lupus), cancers and neurodegenerative diseases. Intermediate filaments (IF) and associated proteins (IFAP) are major substrates of PADs. Here, we focus on the effects of deimination on the polymerization and solubility properties of IF proteins and on the proteolysis and cross-linking of IFAP, to finally expose some features of interest and some limitations of citrullinomes.

Published
2020
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2. Pseudomonas aeruginosa eradicates Staphylococcus aureus by manipulating the host immunity

Author

Pierre-Régis Burgel, Isabelle Sermet-Gaudelus, Louise Jeammet, Gérard Lambeau, Lhousseine Touqui, Dominique Leduc, Michel Chignard, Laurent Guillemot, Clémence Martin, Philippe Morand, Dorota Sands, Yongzheng Wu, Erwan Pernet, Défense innée et inflammation, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Cellule Pasteur UPMC, Institut Pasteur [Paris] (IP)-Sorbonne Université (SU), Université Paris Descartes - Paris 5 (UPD5), Service de pneumologie [CHU Cochin], Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut de pharmacologie moléculaire et cellulaire (IPMC), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Service de Pneumologie et d'Allergologie Pédiatriques, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Régulation du transport anionique transépithélial : de la recherche fondamentale aux pathologies épithéliales (UMR_S 806), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), National Research Institute of Mother and Child [Warsaw, Poland], Service de Bactériologie [CHU Cochin, AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Cochin [AP-HP], This study was supported by France ‘Vaincre la Mucoviscidose’ and ‘DIM Malinf-Region Ile de France’ foundation., We thank Dr R. Ramphal (University of Florida, Gainesville, FL, USA), Dr T. Msadek (Institut Pasteur, Paris), Dr E. Pearlman (Case Western Reserve University, Cleveland, OH, USA), Dr J.C. Olson (West Virginia University, Morgantown, WV, USA), Dr P. Peris (University of València, Spain), Dr M. Gelb (University of Washington, Seattle, WA, USA) for providing PA and SA strains as well as substrate for sPLA2 activity assay and RO032007A compound, Dr C. Schmitt (Hôpital Trousseau, Paris) for providing expectorations of children patients, V. Balloy and M. Nahori (Institut Pasteur, Paris) for technical assistance with intratracheal and intravenous instillation and C. Payre for helpful advices in sPLA2 analyses., Wu, Yongzheng, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris]-Sorbonne Université (SU), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA), and Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Descartes - Paris 5 (UPD5)

Subjects
Cystic Fibrosis, MESH: ADP Ribose Transferases, General Physics and Astronomy, MESH: Staphylococcus aureus / physiology, medicine.disease_cause, [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity, Cystic fibrosis, Mice, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, MESH: Child, MESH: Respiratory Mucosa, MESH: Animals, Child, ADP Ribose Transferases, MESH: Sputum / enzymology, 0303 health sciences, Multidisciplinary, MESH: Bronchi, respiratory system, 3. Good health, MESH: Young Adult, Staphylococcus aureus, Child, Preschool, Pseudomonas aeruginosa, Disease Progression, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, MESH: Disease Progression, Adult, Host immunity, Adolescent, Bacterial Toxins, Guinea Pigs, Bronchi, Respiratory Mucosa, MESH: Epithelial Cells / enzymology, Biology, Group II Phospholipases A2, General Biochemistry, Genetics and Molecular Biology, MESH: Guinea Pigs, Microbiology, Young Adult, 03 medical and health sciences, MESH: Pseudomonas aeruginosa / physiology, medicine, Animals, Humans, MESH: Mice, [SDV.IMM.II] Life Sciences [q-bio]/Immunology/Innate immunity, 030304 developmental biology, MESH: Adolescent, Respiratory tract diseases, MESH: Humans, MESH: Group II Phospholipases A2 / metabolism, 030306 microbiology, MESH: Child, Preschool, Sputum, Epithelial Cells, MESH: Adult, General Chemistry, medicine.disease, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, respiratory tract diseases, MESH: Bacterial Toxins, Immunology, MESH: Cystic Fibrosis / enzymology, Bacterial infection, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, MESH: Cystic Fibrosis / microbiology
Abstract

International audience; Young cystic fibrosis (CF) patients' airways are mainly colonized by Staphylococcus aureus, while Pseudomonas aeruginosa predominates in adults. However, the mechanisms behind this infection switch are unclear. Here, we show that levels of type-IIA-secreted phospholipase A2 (sPLA2-IIA, a host enzyme with bactericidal activity) increase in expectorations of CF patients in an age-dependent manner. These levels are sufficient to kill S. aureus, with marginal effects on P. aeruginosa strains. P. aeruginosa laboratory strains and isolates from CF patients induce sPLA2-IIA expression in bronchial epithelial cells from CF patients (these cells are a major source of the enzyme). In an animal model of lung infection, P. aeruginosa induces sPLA2-IIA production that favours S. aureus killing. We suggest that sPLA2-IIA induction by P. aeruginosa contributes to S. aureus eradication in CF airways. Our results indicate that a bacterium can eradicate another bacterium by manipulating the host immunity.

Published
2014
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