Search

Your search keyword '"MESH: Clostridium Infections"' showing total 16 results
Start Over Descriptor "MESH: Clostridium Infections"
16 results on '"MESH: Clostridium Infections"'

1. In vivo commensal control of Clostridioides difficile virulence

Author

Mario L. Arrieta-Ortiz, Johann Peltier, Andrew B. Onderdonk, Selva Rupa Christinal Immanuel, Narjol Gonzalez-Escalona, Nicholas DiBenedetto, Richard Lavin, Nitin S. Baliga, Marc W. Allard, Maria Hoffman, Abraham L. Sonenshein, Yan Luo, Christopher K Cummins, Brintha P. Girinathan, Lynn Bry, Bruno Dupuy, Jay N. Worley, Mary L. Delaney, Georg K. Gerber, Brigham & Women’s Hospital [Boston] (BWH), Harvard Medical School [Boston] (HMS), National Center for Biotechnology Information (NCBI), Pathogénèse des Bactéries Anaérobies / Pathogenesis of Bacterial Anaerobes (PBA (U-Pasteur_6)), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Institute for Systems Biology [Seattle] (ISB), U.S. Food and Drug Administration (FDA), Harvard-MIT Division of Health Sciences and Technology [Cambridge], Massachusetts Institute of Technology (MIT), Tufts University School of Medicine [Boston], This work was supported by the BWH Precision Medicine Institute, R01AI153605 from the National Institute of Allergy and Infectious Diseases (NIAID) and P30DK034854 from the National Institute of Diabetes, Digestive and Kidney Diseases to L.B., R01AI128215, R01AI141953, and U19AI135976 from NIAID and IIBR-2042948 from the National Science Foundation to N.S.B., B.P.G. was supported by T32 HL007627, and the work of J.N.W. was supported by the Intramural Research Program of the National Library of Medicine, National Institutes of Health. J.P. received support from the Institut Pasteur (Bourse ROUX). This work was conducted by authors from the FDA working as official U.S. Government employees and for which no additional external funding was utilized.

Subjects
MESH: Clostridioides difficile, MESH: Amino Acids, colitis, Systems biology, MESH: Clostridium Infections, [SDV]Life Sciences [q-bio], gnotobiotic mouse, Clostridium sardiniense, Virulence, MESH: Butyrates, Biology, MESH: Virulence, medicine.disease_cause, Microbiology, MESH: Clostridiales, MESH: Fermentation, Clostridia, MESH: Gene Expression Profiling, conventional mouse, In vivo, Virology, MESH: Germ-Free Life, MESH: Severity of Illness Index, medicine, Paraclostridium bifermentans, MESH: Animals, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Colitis, Pathogen, MESH: Mice, MESH: Gene Regulatory Networks, MESH: Gene Expression Regulation, Bacterial, metatranscriptomics, MESH: Symbiosis, Toxin, Host (biology), Clostridioides difficile, MESH: Clostridium, MESH: Arginine, MESH: Cecum, biology.organism_classification, medicine.disease, metabolomics, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, MESH: Systems Biology, Parasitology, carbon source enrichment analysis, anaerobe metabolism
Abstract

International audience; Leveraging systems biology approaches, we illustrate how metabolically distinct species of Clostridia protect against or worsen Clostridioides difficile infection in mice by modulating the pathogen's colonization, growth, and virulence to impact host survival. Gnotobiotic mice colonized with the amino acid fermenter Paraclostridium bifermentans survive infection with reduced disease severity, while mice colonized with the butyrate-producer, Clostridium sardiniense, succumb more rapidly. Systematic in vivo analyses revealed how each commensal alters the gut-nutrient environment to modulate the pathogen's metabolism, gene regulatory networks, and toxin production. Oral administration of P. bifermentans rescues conventional, clindamycin-treated mice from lethal C. difficile infection in a manner similar to that of monocolonized animals, thereby supporting the therapeutic potential of this commensal species. Our findings lay the foundation for mechanistically informed therapies to counter C. difficile disease using systems biology approaches to define host-commensal-pathogen interactions in vivo.

Published
2021
Full Text
View/download PDF

2. Safety and Efficacy of Fidaxomicin and Vancomycin in Children and Adolescents with Clostridioides (Clostridium) difficile Infection: A Phase 3, Multicenter, Randomized, Single-blind Clinical Trial (SUNSHINE)

Author

Claudia Fortuny, Arnaud Petit, Samantha Bosis, Elodie Incera, Bartosz Korczowski, Krisztina Kalocsai, Daniel Bradford, Joost Melis, Stefan Lazar, Rob van Maanen, Rodney Croos-Dabrera, Joshua Wolf, St Jude Children's Research Hospital, Dél-pesti Centrumkórház - Országos Hematológiai és Infektológiai Intézet [Budapest, Hungary], Hospital Sant Joan de Déu [Barcelona], Universitat de Barcelona (UB), Victor Babeş University of Medicine and Pharmacy (UMFT), Università degli Studi di Milano [Milano] (UNIMI), Fondazione IRCCS Ca' Granda - Ospedale Maggiore Policlinico, Rzeszow University (RU Poland), Service d'hématologie-immunologie-oncologie pédiatrique [CHU Trousseau], CHU Trousseau [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Centre de Recherche Saint-Antoine (CR Saint-Antoine), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Saint-Antoine [AP-HP], Astellas Pharma B.V. [Leiden, the Netherlands], Astellas Pharma Development [Northbrook, IL, USA], Gestionnaire, Hal Sorbonne Université, Università degli Studi di Milano = University of Milan (UNIMI), Centre de Recherche Saint-Antoine (CRSA), and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)

Subjects
0301 basic medicine, Microbiology (medical), MESH: Clostridioides difficile, medicine.medical_specialty, medicine.drug_class, MESH: Clostridium Infections, [SDV]Life Sciences [q-bio], 030106 microbiology, Antibiotics, vancomycin, MESH: Clostridioides, 03 medical and health sciences, MESH: Vancomycin, 0302 clinical medicine, Internal medicine, MESH: Anti-Bacterial Agents, MESH: Child, Medicine, Fidaxomicin, 030212 general & internal medicine, Adverse effect, MESH: Treatment Outcome, MESH: Adolescent, MESH: Aged, MESH: Humans, business.industry, MESH: Clostridium, fidaxomicin, MESH: Fidaxomicin, MESH: Child, Preschool, MESH: Adult, MESH: Aminoglycosides, Clostridium difficile, 16. Peace & justice, MESH: Single-Blind Method, Confidence interval, 3. Good health, [SDV] Life Sciences [q-bio], Clinical trial, Infectious Diseases, pediatric, Clostridioides difficile infection, Vancomycin, business, Clostridioides, medicine.drug
Abstract

Background Fidaxomicin, a narrow-spectrum antibiotic approved for Clostridioides (Clostridium) difficile infection (CDI) in adults, is associated with lower rates of recurrence than vancomycin; however, pediatric data are limited. This multicenter, investigator-blind, phase 3, parallel-group trial assessed the safety and efficacy of fidaxomicin in children. Methods Patients aged Results Of 148 patients randomized, 142 were treated (30 Conclusions Compared with vancomycin, fidaxomicin was well tolerated and demonstrated significantly higher rates of GC in children and adolescents with CDI. Clinical Trials Registration NCT02218372

Published
2020
Full Text
View/download PDF

3. Characterization of Clostridium Perfringens Isolates Collected from Three Agricultural Biogas Plants over a One-Year Period

Author

Derongs, Lorine, Druilhe, Céline, Ziebal, Christine, Le Maréchal, Caroline, Pourcher, Anne-Marie, Optimisation des procédés en Agriculture, Agroalimentaire et Environnement (UR OPAALE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire de Ploufragan-Plouzané-Niort [ANSES], Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), and ADEME (grant number 1606C0022)

Subjects
MESH: Clostridium perfringens, Clostridium perfringens, MESH: Clostridium Infections, MESH: Manure, mesophilic anaerobic digestion, lcsh:R, lcsh:Medicine, C. perfringens, biochemical phenomena, metabolism, and nutrition, antimicrobial susceptibility, toxinotypes, MESH: Anti-Bacterial Agents, manure, digestate, [SDE]Environmental Sciences, MESH: Biofuels
Abstract

Digestate produced by agricultural biogas plants (BGPs) may contain pathogenic bacteria. Among them, Clostridium perfringens deserves particular attention due to its ability to grow under anaerobic conditions and persist in amended soil. The aim of this study was to examine the potential pathogenicity and the antimicrobial resistance of C. perfringens in manure and digestate collected from three agricultural biogas plants (BGPs). A total of 157 isolates (92 from manure, 65 from digestate) were screened for genes encoding seven toxins (cpa, cpb, etx, iapcpe, netB, and cpb2). The 138 cpa positive isolates were then screened for tetA(P), tetB(P), tet(M), and erm(Q) genes and tested for antimicrobial susceptibility. The toxinotypes identified in both manure and digestate were type A (78.3% of the isolates), type G (16.7%), type C (3.6%), and type D (1.4%), whereas none of the isolates were type F. Moreover, half of the isolates carried the cpb2 gene. The overall prevalence of tetA(P) gene alone, tetA(P)-tetB(P) genes, and erm(Q) gene was 31.9, 34.8, and 6.5%, respectively. None of the isolates harbored the tet(M) gene. Multiple antimicrobial resistant isolates were found in samples that were collected from all the manure and digestates. Among them, 12.3% were highly resistant to some of the antibiotics tested, especially to clindamycin (MIC &ge, 16 &micro, g/mL) and tilmicosin (MIC &gt, 64 &micro, g/mL). Some isolates were highly resistant to antibiotics used in human medicine, including vancomycin (MIC &gt, 8 &micro, g/mL) and imipenem (MIC &gt, g/mL). These results suggest that digestate may be a carrier of the virulent and multidrug resistant C. perfringens.

Published
2020
Full Text
View/download PDF

4. Cwp19 Is a Novel Lytic Transglycosylase Involved in Stationary-Phase Autolysis Resulting in Toxin Release in Clostridium difficile

Author

Jean-Louis Pons, Bruno Dupuy, Marie-Pierre Chapot-Chartier, Marie-José Butel, Imane El Meouche, Sandra Wydau-Dematteis, René Lai-Kuen, Johann Peltier, Audrey Hamiot, François Fenaille, Bruno Saubaméa, Pascal Courtin, Ecosystème intestinal, probiotiques, antibiotiques (EA 4065), Université Paris Descartes - Paris 5 (UPD5), Groupe de Recherche sur l'Adaptation Microbienne (GRAM 2.0), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Pathogénèse des Bactéries Anaérobies / Pathogenesis of Bacterial Anaerobes (PBA (U-Pasteur_6)), Institut Pasteur [Paris]-Université Paris Diderot - Paris 7 (UPD7), Université Paris Diderot - Paris 7 (UPD7), Plateau technique Imagerie Cellulaire et Moléculaire, Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Etude du Métabolisme des Médicaments (LEMM), Service de Pharmacologie et Immunoanalyse (SPI), Médicaments et Technologies pour la Santé (MTS), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Médicaments et Technologies pour la Santé (MTS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), This work was supported by the University of Rouen and the University of Paris-Descartes. J. Peltier is funded by a Pasteur-Roux fellowship. The work of P. Courtin andM.-P. Chapot-Chartier was supported by INRA., Institut Pasteur [Paris] (IP)-Université Paris Diderot - Paris 7 (UPD7), Faculté de Pharmacie de Paris - Université Paris Descartes (UPD5 Pharmacie), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Médicaments et Technologies pour la Santé (MTS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Université de Caen Normandie (UNICAEN), Université Paris Descartes - Faculté de Pharmacie de Paris (UPD5 Pharmacie), Peltier, Johann, and Martin, Marie

Subjects
autolysins, lytic transglycosylase, peptidoglycan, surface proteins, toxins, clostridium difficile, 0301 basic medicine, autolysin, Autolysis (biology), toxins secretion, peptidoglycan hydrolase, Applied Microbiology, MESH: Catalytic Domain, medicine.disease_cause, Cwp19, chemistry.chemical_compound, Cell Wall, Catalytic Domain, glucose, MESH: Bacterial Proteins, MESH: Gene Expression Regulation, Bacterial, Chemistry, Cell autolysis, Clostridium difficile, QR1-502, 3. Good health, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Lytic cycle, MESH: Peptidoglycan Glycosyltransferase, Research Article, MESH: Bacteriolysis, MESH: Clostridium Infections, 030106 microbiology, Microbiology, 03 medical and health sciences, Bacteriolysis, MESH: Cell Wall, Bacterial Proteins, Virology, Genetics, medicine, Humans, Secretion, Molecular Biology, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, MESH: Clostridium difficile, MESH: Humans, Clostridioides difficile, Toxin, Lytic transglycosylase activity, Gene Expression Regulation, Bacterial, Clostridium Infections, Peptidoglycan Glycosyltransferase, Peptidoglycan
Abstract

Clostridium difficile is the major etiologic agent of antibiotic-associated intestinal disease. Pathogenesis of C. difficile is mainly attributed to the production and secretion of toxins A and B. Unlike most clostridial toxins, toxins A and B have no signal peptide, and they are therefore secreted by unusual mechanisms involving the holin-like TcdE protein and/or autolysis. In this study, we characterized the cell surface protein Cwp19, a newly identified peptidoglycan-degrading enzyme containing a novel catalytic domain. We purified a recombinant His6-tagged Cwp19 protein and showed that it has lytic transglycosylase activity. Moreover, we observed that Cwp19 is involved in cell autolysis and that a C. difficile cwp19 mutant exhibited delayed autolysis in stationary phase compared to the wild type when bacteria were grown in brain heart infusion (BHI) medium. Wild-type cell autolysis is correlated to strong alterations of cell wall thickness and integrity and to release of cytoplasmic material. Furthermore, we demonstrated that toxins were released into the extracellular medium as a result of Cwp19-induced autolysis when cells were grown in BHI medium. In contrast, Cwp19 did not induce autolysis or toxin release when cells were grown in tryptone-yeast extract (TY) medium. These data provide evidence for the first time that TcdE and bacteriolysis are coexisting mechanisms for toxin release, with their relative contributions in vitro depending on growth conditions. Thus, Cwp19 is an important surface protein involved in autolysis of vegetative cells of C. difficile that mediates the release of the toxins from the cell cytosol in response to specific environment conditions., IMPORTANCE Clostridium difficile-associated disease is mainly known as a health care-associated infection. It represents the most problematic hospital-acquired infection in North America and Europe and exerts significant economic pressure on health care systems. Virulent strains of C. difficile generally produce two toxins that have been identified as the major virulence factors. The mechanism for release of these toxins from bacterial cells is not yet fully understood but is thought to be partly mediated by bacteriolysis. Here we identify a novel peptidoglycan hydrolase in C. difficile, Cwp19, exhibiting lytic transglycosylase activity. We show that Cwp19 contributes to C. difficile cell autolysis in the stationary phase and, consequently, to toxin release, most probably as a response to environmental conditions such as nutritional signals. These data highlight that Cwp19 constitutes a promising target for the development of new preventive and curative strategies.

Published
2018
Full Text
View/download PDF

5. First isolation of Clostridium indolis in a patient with chronic osteitis: a case report and literature review of human infections related to Clostridium saccharolyticum group species

Author

Michel-Robert Popoff, Romain Lotte, Michel Carles, Philippe Bouvet, Regis Bernard de Dompsure, Antonin Bal, Nicolas Degand, Laurène Lotte, Raymond Ruimy, Centre Hospitalier Universitaire de Nice (CHU Nice), Centre méditerranéen de médecine moléculaire (C3M), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de Référence des Bactéries Anaérobies et Botulisme - National Reference Center Anaerobic Bacteria and Botulism (CNR), Institut Pasteur [Paris], Hôpital Archet 2 [Nice] (CHU), This work has been supported by Fondation Recherche Médicale (FRM) (Grant#FDM20150632804 to Romain Lotte), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA), and Institut Pasteur [Paris] (IP)

Subjects
0301 basic medicine, [SDV]Life Sciences [q-bio], Gene Expression, Fractures, Bone, Clostridium, RNA, Ribosomal, 16S, Anaerobiosis, MESH: Phylogeny, MESH: Bacterial Proteins, Phylogeny, Osteitis, MESH: Microbial Sensitivity Tests, biology, Isolation (microbiology), Anti-Bacterial Agents, 3. Good health, MESH: RNA, Ribosomal, 16S, Infectious Diseases, [SDV.TOX]Life Sciences [q-bio]/Toxicology, Emerging pathogen, MESH: Osteitis, Female, Adult, MESH: Gene Expression, MESH: Clostridium Infections, 030106 microbiology, Bacillus, Clostridium saccharolyticum, Microbial Sensitivity Tests, Microbiology, 03 medical and health sciences, Bacterial Proteins, MESH: Enzyme Assays, MESH: Anti-Bacterial Agents, MESH: Anaerobiosis, medicine, Humans, [Clostridium] indolis, Clinical significance, Enzyme Assays, MESH: Humans, MESH: Clostridium, MESH: Chronic Disease, MESH: Fractures, Bone, MESH: Adult, biology.organism_classification, medicine.disease, MESH: Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Chronic Disease, Clostridium Infections, MESH: Female
Abstract

International audience; Clostridium indolis is an anaerobic spore-forming Gram-positive bacillus belonging to the Clostridium saccharolyticum group. Its clinical significance in human remains poorly known. We describe the first case of osteitis related to C. indolis, identified by MALDI-TOF mass spectrometry and provide a literature review of human infections related to C. saccharolyticum group species.

Published
2016
Full Text
View/download PDF

6. Foot Infection by Clostridium sordellii: Case Report and Review of 15 Cases in France

Author

Michel-R. Popoff, Jean Sautereau, Christiane Bouchier, Alain Le Coustumier, Francine Mory, Philippe Bouvet, Centre National de Référence des Bactéries Anaérobies et Botulisme - National Reference Center Anaerobic Bacteria and Botulism (CNR), Institut Pasteur [Paris], Centre hospitalier Jean Rougier, Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Génomique (Plate-Forme) - Genomics Platform, We thank French private and hospital laboratories for sending Clostridium sordellii isolates to the NRC. Maria Manich, Marie Bedora-Faure, and Guylène K'Ouas from the NRC are warmly thanked for excellent technical assistance., Centre National de Référence des Bactéries Anaérobies et du Botulisme - Bactéries Anaérobies et Toxines ( CNR ), Centre Hospitalier Régional Universitaire de Nancy ( CHRU Nancy ), Génomique (Plate-Forme), and Institut Pasteur [Paris] (IP)

Subjects
Male, MESH : Molecular Sequence Data, MESH: Clostridium sordellii, [ SDV.TOX ] Life Sciences [q-bio]/Toxicology, MESH : Aged, Clostridium sordellii, Case Reports, 0302 clinical medicine, MESH: Aged, 80 and over, Medicine, MESH : Female, 030212 general & internal medicine, MESH : Anti-Bacterial Agents, Aged, 80 and over, MESH: Aged, 0303 health sciences, MESH: Middle Aged, biology, Clostridium Infections, MESH : Adult, Middle Aged, 3. Good health, Anti-Bacterial Agents, [SDV.TOX]Life Sciences [q-bio]/Toxicology, Female, France, Foot (unit), Microbiology (medical), Adult, MESH : Male, MESH: Clostridium Infections, Molecular Sequence Data, MESH: Foot Diseases, Microbiology, Foot Diseases, 03 medical and health sciences, MESH: Anti-Bacterial Agents, Humans, MESH : Middle Aged, Lethal toxin, MESH : Aged, 80 and over, MESH : Foot Diseases, MESH : France, Aged, MESH: Molecular Sequence Data, MESH: Humans, 030306 microbiology, business.industry, MESH : Humans, MESH: Adult, MESH : Clostridium sordellii, biology.organism_classification, Virology, MESH: Male, Clostridium septicum, MESH : Clostridium Infections, MESH: France, business, MESH: Female
Abstract

We report a case of foot infection by Clostridium sordellii and review 15 human infections registered at a Reference Center in France during the period 1998 to 2011. All strains were found nontoxigenic, lacking the lethal toxin gene coding for TcsL. Like Clostridium septicum , several C. sordellii infections were associated with intestinal neoplasms.

Published
2015
Full Text
View/download PDF

7. Lumbar Discitis Caused by Clostridium perfringens

Author

Nicolas Degand, Philippe Bouvet, Guillaume Baudin, Laurène Lotte, Michel R. Popoff, Eric Cua, Romain Lotte, Pierre-Marie Roger, Raymond Ruimy, Université Nice Sophia Antipolis ( UNS ), Université Côte d'Azur ( UCA ), CHU Nice, Centre National de Référence des Bactéries Anaérobies et du Botulisme - Bactéries Anaérobies et Toxines ( CNR ), Institut Pasteur [Paris], Hôpital l'Archet, Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), Centre Hospitalier Universitaire de Nice (CHU Nice), Centre National de Référence des Bactéries Anaérobies et Botulisme - National Reference Center Anaerobic Bacteria and Botulism (CNR), Université Nice Sophia Antipolis (1965 - 2019) (UNS), and Institut Pasteur [Paris] (IP)

Subjects
Clostridium perfringens, [ SDV.TOX ] Life Sciences [q-bio]/Toxicology, MESH : Genotype, Case Reports, Intervertebral Disc Degeneration, MESH : Discitis, medicine.disease_cause, Polymerase Chain Reaction, MESH: Genotype, MESH: Aged, 80 and over, Rare case, MESH : Tomography, X-Ray Computed, MESH : Clindamycin, MESH : Female, Lumbar discitis, MESH : Anti-Bacterial Agents, MESH : Polymerase Chain Reaction, MESH : Intervertebral Disc Displacement, Aged, 80 and over, MESH: Clostridium perfringens, MESH: Microbial Sensitivity Tests, MESH: Clindamycin, Clindamycin, 3. Good health, Molecular analysis, Anti-Bacterial Agents, [SDV.TOX]Life Sciences [q-bio]/Toxicology, Female, MESH: Tomography, X-Ray Computed, MESH: Spine, Intervertebral Disc Displacement, medicine.drug, Microbiology (medical), MESH : Clostridium perfringens, Discitis, Genotype, MESH: Clostridium Infections, Bacterial Toxins, Microbial Sensitivity Tests, Biology, beta-Lactams, Microbiology, MESH : beta-Lactams, MESH: beta-Lactams, MESH: Anti-Bacterial Agents, medicine, MESH : Spine, Humans, Elderly patient, MESH : Aged, 80 and over, MESH: Humans, MESH : Intervertebral Disc Degeneration, MESH: Intervertebral Disc Displacement, Toxin, MESH : Humans, MESH: Polymerase Chain Reaction, medicine.disease, Spine, MESH: Intervertebral Disc Degeneration, MESH : Clostridium Infections, MESH: Discitis, MESH: Bacterial Toxins, MESH : Bacterial Toxins, Clostridium Infections, MESH : Microbial Sensitivity Tests, Tomography, X-Ray Computed, MESH: Female
Abstract

We report here a rare case of chronic lumbar discitis caused by Clostridium perfringens in an elderly patient that was treated with a combination of β-lactams and clindamycin. Molecular analysis performed on the strain revealed an unusual toxin gene pattern.

Published
2014
Full Text
View/download PDF

8. Correlation between in vitro cytotoxicity and in vivo lethal activity in mice of epsilon toxin mutants from Clostridium perfringens

Author

Dorca Arévalo, Jonatan, Pauillac, Serge, Díaz Hidalgo, Laura, Martín Satué, Mireia, Popoff, Michel R., Blasi Cabús, Joan, Institut d'Investigació Biomèdica de Bellvitge [Barcelone] ( IDIBELL ), Universitat de Barcelona ( UB ), Bactéries anaérobies et Toxines, Institut Pasteur [Paris], This work was supported by grant SAF2011-27566 (MINECO, Spanish Government) and grant 2009 SGR 152 from AGAUR (Generalitat de Catalunya), The authors thank Inmaculada Gómez de Aranda and the CCiTUB Biology Unit of the Campus de Bellvitge for their technical assistance. We are also grateful to Dr. Mark S. McClain (Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee) for his valuable comments and discussions., Institut d'Investigació Biomèdica de Bellvitge [Barcelone] (IDIBELL), Universitat de Barcelona (UB), Institut Pasteur [Paris] (IP), and Universitat de Barcelona

Subjects
Male, Clostridium perfringens, Toxicology, Mouse models, MESH : Green Fluorescent Proteins, Madin Darby Canine Kidney Cells, MESH: Dogs, Mice, MESH: Structure-Activity Relationship, Membrane proteins, Toxin Binding, Toxins, MESH: Animals, Kidney Tubules, Distal, lcsh:Science, MESH: Clostridium perfringens, Brain, Veterinary Diseases, Neurology, Medical Microbiology, MESH: Epithelial Cells, [SDV.TOX]Life Sciences [q-bio]/Toxicology, Neurotoxicology, MESH : Clostridium perfringens, Cell Physiology, Multiple Sclerosis, MESH: Gene Expression, Recombinant Fusion Proteins, Primary Cell Culture, Immunology, Toxic Agents, Microbiology, Structure-Activity Relationship, MESH: Green Fluorescent Proteins, MESH: Kidney Tubules, Distal, MESH: Recombinant Fusion Proteins, MESH : Primary Cell Culture, Microbial Pathogens, [ SDV.BC ] Life Sciences [q-bio]/Cellular Biology, lcsh:R, Biology and Life Sciences, Cell binding, Survival Analysis, MESH : Kidney Tubules, Distal, Cell membranes, Confocal microscopy, MESH : Gene Expression, MESH : Brain, Mutation, Clostridium Infections, MESH: Enterotoxemia, Veterinary Science, lcsh:Q, Membranes cel·lulars, Veterinary Microbiology, [ SDV.TOX ] Life Sciences [q-bio]/Toxicology, Veterinary Toxicology, Gene Expression, lcsh:Medicine, Epithelial cells, MESH: Blood-Brain Barrier, MESH : Blood-Brain Barrier, MESH : Dogs, Molecular Cell Biology, MESH : Structure-Activity Relationship, Medicine and Health Sciences, Cèl·lules epitelials, MESH : Epithelial Cells, Blood-Brain Barrier, MESH: Survival Analysis, MESH : Mutation, Research Article, MESH: Mutation, MESH : Recombinant Fusion Proteins, MESH: Biological Transport, MESH: Clostridium Infections, MESH : Male, Bacterial Toxins, Green Fluorescent Proteins, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Autoimmune Diseases, MESH: Primary Cell Culture, MESH: Brain, Dogs, MESH : Mice, Animals, MESH: Mice, MESH : Enterotoxemia, MESH : Madin Darby Canine Kidney Cells, MESH: Madin Darby Canine Kidney Cells, Biological Transport, Kidneys, Cell Biology, Demyelinating Disorders, MESH: Male, MESH : Clostridium Infections, MESH : Biological Transport, MESH: Bacterial Toxins, Membrane Trafficking, Toxines, MESH : Bacterial Toxins, Clinical Immunology, MESH : Animals, MESH : Survival Analysis, Enterotoxemia
Abstract

International audience; Epsilon toxin (Etx) from Clostridium perfringens is a pore-forming protein with a lethal effect on livestock, producing severe enterotoxemia characterized by general edema and neurological alterations. Site-specific mutations of the toxin are valuable tools to study the cellular and molecular mechanism of the toxin activity. In particular, mutants with paired cysteine substitutions that affect the membrane insertion domain behaved as dominant-negative inhibitors of toxin activity in MDCK cells. We produced similar mutants, together with a well-known non-toxic mutant (Etx-H106P), as green fluorescent protein (GFP) fusion proteins to perform in vivo studies in an acutely intoxicated mouse model. The mutant (GFP-Etx-I51C/A114C) had a lethal effect with generalized edema, and accumulated in the brain parenchyma due to its ability to cross the blood-brain barrier (BBB). In the renal system, this mutant had a cytotoxic effect on distal tubule epithelial cells. The other mutants studied (GFP-Etx-V56C/F118C and GFP-Etx-H106P) did not have a lethal effect or cross the BBB, and failed to induce a cytotoxic effect on renal epithelial cells. These data suggest a direct correlation between the lethal effect of the toxin, with its cytotoxic effect on the kidney distal tubule cells, and the ability to cross the BBB.

Published
2014
Full Text
View/download PDF

9. Clostridium perfringens epsilon toxin: a malevolent molecule for animals and man?

Author

Gillian Barth, Michel R. Popoff, Bradley G. Stiles, Holger Barth, Wilson College, Universität Ulm - Ulm University [Ulm, Allemagne], Bactéries anaérobies et Toxines, Institut Pasteur [Paris], G.B. and B.G.S. thank Wilson College for generous use of facilities that include computers, copiers and library services, University of Ulm, and Institut Pasteur [Paris] (IP)

Subjects
Clostridium perfringens, Health, Toxicology and Mutagenesis, receptor, [ SDV.TOX ] Life Sciences [q-bio]/Toxicology, lcsh:Medicine, Review, Toxicology, medicine.disease_cause, Mice, MESH : Cattle, therapeutics, MESH: Animals, Receptor, MESH: Clostridium perfringens, 0303 health sciences, protein toxin, Goats, Vaccination, vaccines, animal models, 3. Good health, MESH: Cattle, [SDV.TOX]Life Sciences [q-bio]/Toxicology, MESH : Clostridium perfringens, MESH: Clostridium Infections, Bacterial Toxins, MESH: Sheep, Biology, MESH : Sheep, MESH: Goats, Enteritis, Microbiology, 03 medical and health sciences, In vivo, MESH : Mice, medicine, Animals, Humans, MESH: Mice, 030304 developmental biology, cell culture, MESH: Humans, Sheep, 030306 microbiology, Toxin, MESH : Humans, lcsh:R, MESH: Vaccination, Clostridium perfringens epsilon toxin, medicine.disease, Virology, MESH : Disease Models, Animal, In vitro, MESH : Clostridium Infections, Disease Models, Animal, MESH : Goats, MESH : Vaccination, MESH: Bacterial Toxins, Cell culture, MESH : Bacterial Toxins, Clostridium Infections, Cattle, MESH : Animals, MESH: Disease Models, Animal
Abstract

International audience; Clostridium perfringens is a prolific, toxin-producing anaerobe causing multiple diseases in humans and animals. One of these toxins is epsilon, a 33 kDa protein produced by Clostridium perfringens (types B and D) that induces fatal enteric disease of goats, sheep and cattle. Epsilon toxin (Etx) belongs to the aerolysin-like toxin family. It contains three distinct domains, is proteolytically-activated and forms oligomeric pores on cell surfaces via a lipid raft-associated protein(s). Vaccination controls Etx-induced disease in the field. However, therapeutic measures are currently lacking. This review initially introduces C. perfringens toxins, subsequently focusing upon the Etx and its biochemistry, disease characteristics in various animals that include laboratory models (in vitro and in vivo), and finally control mechanisms (vaccines and therapeutics)

Published
2013
Full Text
View/download PDF

10. Adaptive strategies and pathogenesis of Clostridium difficile from in vivo transcriptomics

Author

Laxmee Caleechum, Anne Collignon, Frédéric Barbut, Sylvie Bouttier, Marc Monot, Fátima C. Pereira, Cécile Denève, Claire Janoir, Sandra Hoys, Bruno Dupuy, Adriano O. Henriques, Diana Chapeton-Montes, EA 4043, Université Paris-Sud - Paris 11 (UP11), CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Pathogénèse des Bactéries Anaérobies / Pathogenesis of Bacterial Anaerobes (PBA (U-Pasteur_6)), Institut Pasteur [Paris]-Université Paris Diderot - Paris 7 (UPD7), Bactéries anaérobies et Toxines, Institut Pasteur [Paris], Instituto de Tecnologia Química e Biológica António Xavier (ITQB), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Service de Microbiologie [Hôpital Jean Verdier], Hôpital Jean Verdier [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), This work was funded by the European Community's Seventh Framework Programme HYPERDIFF (grant HEALTH-F3-2008-223585), the Institut Pasteur, the Université Paris-Sud, and the Fundação para a Ciência e a Tecnologia (FCT) (grant Pest-C/EQB/LA0006/2011). C.D. and D.C.-M. were supported by doctoral fellowships from the French Ministry of Higher Education and Research, and F.P. was the recipient of a fellowship from the FCT (grant SFRH/BD/45459/08)., European Project: 223585,EC:FP7:HEALTH,FP7-HEALTH-2007-B,HYPERDIFF(2008), Institut Pasteur [Paris] (IP)-Université Paris Diderot - Paris 7 (UPD7), Institut Pasteur [Paris] (IP), National reference laboratory for clostridium difficile, Université Pierre et Marie Curie - Paris 6 (UPMC)-AP-HP - Hôpital Saint-Antoine, Universidade Nova de Lisboa (NOVA), Hôpital Jean Verdier [Bondy], Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP), Bactéries, Pathogènes et Santé (UBaPS), Faculté de Pharmacie, Université Paris-Sud - Paris 11 (UP11)-Université Paris-Sud - Paris 11 (UP11), and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)

Subjects
[SDV]Life Sciences [q-bio], MESH: Virulence, MESH: Genome, Bacterial, Transcriptome, Mice, MESH: Up-Regulation, MESH: Animals, MESH: Stress, Physiological, Cecum, MESH: Bacterial Proteins, Regulation of gene expression, 0303 health sciences, MESH: Gene Expression Regulation, Bacterial, Virulence, MESH: Peptidoglycan, Clostridium difficile, Adaptation, Physiological, Up-Regulation, Infectious Diseases, Host adaptation, MESH: Mutation, Virulence Factors, MESH: Clostridium Infections, Immunology, Peptidoglycan, Biology, Microbiology, 03 medical and health sciences, Bacterial Proteins, In vivo, Stress, Physiological, Animals, Author Correction, Gene, MESH: Mice, MESH: Clostridium difficile, 030304 developmental biology, MESH: Virulence Factors, Microarray analysis techniques, 030306 microbiology, Clostridioides difficile, MESH: Cecum, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Gene Expression Regulation, Bacterial, Molecular Pathogenesis, MESH: Adaptation, Physiological, Mutation, Clostridium Infections, Parasitology, Genome, Bacterial
Abstract

Clostridium difficile is currently the major cause of nosocomial intestinal diseases associated with antibiotic therapy in adults. In order to improve our knowledge of C. difficile -host interactions, we analyzed the genome-wide temporal expression of C. difficile 630 genes during the first 38 h of mouse colonization to identify genes whose expression is modulated in vivo , suggesting that they may play a role in facilitating the colonization process. In the ceca of the C. difficile -monoassociated mice, 549 genes of the C. difficile genome were differentially expressed compared to their expression during in vitro growth, and they were distributed in several functional categories. Overall, our results emphasize the roles of genes involved in host adaptation. Colonization results in a metabolic shift, with genes responsible for the fermentation as well as several other metabolic pathways being regulated inversely to those involved in carbon metabolism. In addition, several genes involved in stress responses, such as ferrous iron uptake or the response to oxidative stress, were regulated in vivo . Interestingly, many genes encoding conserved hypothetical proteins (CHP) were highly and specifically upregulated in vivo . Moreover, genes for all stages of sporulation were quickly induced in vivo , highlighting the observation that sporulation is central to the persistence of C. difficile in the gut and to its ability to spread in the environment. Finally, we inactivated two genes that were differentially expressed in vivo and evaluated the relative colonization fitness of the wild-type and mutant strains in coinfection experiments. We identified a CHP as a putative colonization factor, supporting the suggestion that the in vivo transcriptomic approach can unravel new C. difficile virulence genes.

Published
2013
Full Text
View/download PDF

11. Clostridium difficile infections: do we know the real dimensions of the problem?

Author

Christian Michelet, Matthieu Revest, Sylvie Buffet-Bataillon, Pierre Tattevin, Pierre-Yves Donnio, Fonction structure et inactivation d'ARN bactérien, Service des maladies infectieuses et réanimation médicale [Rennes] = Infectious Disease and Intensive Care [Rennes], CHU Pontchaillou [Rennes]-CHU Pontchaillou [Rennes]-Fonction, structure et inactivation d'ARN bactériens, Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Unité de contrôle infectieux, Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Hôpital Pontchaillou-CHU Pontchaillou [Rennes]-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Hôpital Pontchaillou-CHU Pontchaillou [Rennes], Microbiologie, Unité de maladies infectieuses, CHU Pontchaillou [Rennes]-CHU Pontchaillou [Rennes], Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Université de Rennes (UR)-Hôpital Pontchaillou-CHU Pontchaillou [Rennes]-Université de Rennes (UR)-Hôpital Pontchaillou-CHU Pontchaillou [Rennes], Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), and Coval, Nathalie

Subjects
genetic structures, polymerase chain reaction, Toxin B, Disease, Global Health, 0302 clinical medicine, Epidemiology, Pharmacology (medical), 030212 general & internal medicine, MESH: Incidence, Cross Infection, 0303 health sciences, Incidence, Diagnostic test, General Medicine, Clostridium difficile, [SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences, Clostridium difficile infections, Anti-Bacterial Agents, 3. Good health, Community-Acquired Infections, [SDV.SP] Life Sciences [q-bio]/Pharmaceutical sciences, MESH: Diarrhea, Infectious Diseases, MESH: Community-Acquired Infections, Homogeneous, Diagnostic tests, Diarrhea, Microbiology (medical), medicine.medical_specialty, MESH: Clostridium Infections, Enzyme immunoassay, 03 medical and health sciences, Glutamate dehydrogenase, Antibiotic therapy, MESH: Anti-Bacterial Agents, medicine, Humans, Antibiotic use, Intensive care medicine, MESH: Clostridium difficile, MESH: Humans, Clostridioides difficile, 030306 microbiology, business.industry, MESH: Cross Infection, Surgery, Clostridium Infections, business, MESH: World Health
Abstract

International audience; Clostridium difficile infection (CDI) is the primary cause of nosocomial diarrhoea in industrialised countries, usually occurring as a complication of antibiotic therapy in elderly patients. Landmark events contributed to boosting interest in CDI over the last 10 years, including the emergence of unusually severe and recurrent CDI due to the NAP1/BI/027 strain, as well as reports suggesting that CDI is also significantly encountered in patients previously considered at no risk, such as community-acquired CDI in patients with no recent antibiotic use, or CDI during pregnancy. Despite this growing interest from the medical community, we do not know the real dimensions of the disease for the following reasons: (i) despite comprehensive guidelines published in Europe and in the USA, most laboratories still use diagnostic tests with suboptimal sensitivity as a 'rule-out' test, hence a significant proportion of CDIs remain undiagnosed; (ii) use of PCR as a stand-alone test by others will probably overestimate the real incidence of CDI and jeopardise any comparison between institutions with different diagnostic procedures; and (iii) transversal studies, with optimum design and diagnostic tests, are rapidly outdated due to the dramatic changes in CDI epidemiology that may occur from one year to another. To get an accurate picture of the real dimensions of the CDI issue, we need more systematic use of an adequate and homogeneous diagnostic strategy in the field as well as the implementation of continuous monitoring of CDI incidence through surveillance programmes.

Published
2013
Full Text
View/download PDF

12. Regulation of toxin synthesis in Clostridium botulinum and Clostridium tetani

Author

Cecile Deneve, Christelle Mazuet, Michel R. Popoff, Chloé Connan, Bactéries anaérobies et Toxines, Institut Pasteur [Paris] (IP), Centre National de Référence des Bactéries Anaérobies et Botulisme - National Reference Center Anaerobic Bacteria and Botulism (CNR), and Institut Pasteur [Paris]

Subjects
MESH: Sequence Analysis, DNA, Botulinum Toxins, Clostridium tetani, Operon, Toxicology, medicine.disease_cause, Clsotridfium tetani, chemistry.chemical_compound, Plasmid, Sigma factor, Clostridium botulinum, Neurotoxin, MESH: Microbial Sensitivity Tests, 0303 health sciences, MESH: DNA, Ribosomal, Corresponding author, botulinum neurotoxin, Quorum Sensing, 3. Good health, MESH: RNA, Ribosomal, 16S, MESH: Young Adult, [SDV.TOX]Life Sciences [q-bio]/Toxicology, Regulation, Plasmids, DNA, Bacterial, MESH: Clostridium Infections, Sigma Factor, Biology, MESH: Bacterial Typing Techniques, Microbiology, 03 medical and health sciences, Bacterial Proteins, Tetanus Toxin, MESH: Anti-Bacterial Agents, MESH: Wound Infection, medicine, TetR, Gene, 030304 developmental biology, MESH: Molecular Sequence Data, MESH: Humans, MESH: Clostridium, 030306 microbiology, Gene Expression Regulation, Bacterial, MESH: DNA, Bacterial, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, MESH: Male, chemistry, MESH: Wounds and Injuries, Genes, Bacterial, Genetic Loci, Trans-Activators
Abstract

Botulinum and tetanus neurotoxins are structurally and functionally related proteins that are potent inhibitors of neuroexocytosis. Botulinum neurotoxin (BoNT) associates with non-toxic proteins (ANTPs) to form complexes of various sizes, whereas tetanus toxin (TeNT) does not form any complex. The BoNT and ANTP genes are clustered in a DNA segment called the botulinum locus, which has different genomic localization (chromosome, plasmid, phage) in the various Clostridium botulinum types and subtypes. The botulinum locus genes are organized in two polycistronic operons ( ntnh-bont and ha/orfX operons) transcribed in opposite orientations. A gene called botR lying between the two operons in C. botulinum type A encodes an alternative sigma factor which regulates positively the synthesis of BoNT and ANTPs at the late exponential growth phase and beginning of the stationary phase. In Clostridium tetani , the gene located immediately upstream of tent encodes a positive regulatory protein, TetR, which is related to BotR. C. botulinum and C. tetani genomes contain several two-component systems and predicted regulatory orphan genes. In C. botulinum type A, four two-component systems have been found that positively or negatively regulate the synthesis of BoNT and ANTPs independently of BotR/A. The synthesis of neurotoxin in Clostridia seems to be under the control of complex network of regulation.

Published
2012
Full Text
View/download PDF

13. Clostridium celerecrescens, often misidentified as 'Clostridium clostridioforme group,' is involved in rare human infection cases

Author

Philippe Bouvet, Michel R. Popoff, Guylène K'ouas, Alain Le Coustumier, Centre National de Référence des Bactéries Anaérobies et du Botulisme - Bactéries Anaérobies et Toxines ( CNR ), Institut Pasteur [Paris], Centre hospitalier Jean Rougier, The authors warmly thank the CRBIP (Biological Resource Centre of Institut Pasteur, Paris) for the gift of the type strain of Clostridium lavalense CIP 109511T., Centre National de Référence des Bactéries Anaérobies et Botulisme - National Reference Center Anaerobic Bacteria and Botulism (CNR), and Institut Pasteur [Paris] (IP)

Subjects
Male, MESH: Sequence Analysis, DNA, MESH : Molecular Sequence Data, [ SDV.TOX ] Life Sciences [q-bio]/Toxicology, Clostridium celerecrescens, “Clostridium clostridioforme group”, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, RNA, Ribosomal, 16S, Misidentification, MESH : Clostridium, MESH : DNA, Ribosomal, MESH : DNA, Bacterial, MESH : Anti-Bacterial Agents, 0303 health sciences, MESH: Microbial Sensitivity Tests, MESH: DNA, Ribosomal, biology, General Medicine, 3. Good health, Anti-Bacterial Agents, Bacterial Typing Techniques, MESH : Wounds and Injuries, MESH: RNA, Ribosomal, 16S, [ SDV.MHEP.MI ] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Infectious Diseases, MESH: Young Adult, [SDV.TOX]Life Sciences [q-bio]/Toxicology, Clostridium clostridioforme, Microbiology (medical), DNA, Bacterial, Clostridium species, Rare Clostridium species, MESH: Clostridium Infections, MESH : Male, Molecular Sequence Data, MESH : Young Adult, Microbial Sensitivity Tests, DNA, Ribosomal, MESH: Bacterial Typing Techniques, Microbiology, 03 medical and health sciences, Young Adult, MESH: Anti-Bacterial Agents, MESH: Wound Infection, Humans, 030304 developmental biology, Clostridium, MESH: Humans, MESH: Molecular Sequence Data, 030306 microbiology, MESH: Clostridium, MESH : Humans, Sequence Analysis, DNA, biology.organism_classification, 16S ribosomal RNA, MESH: DNA, Bacterial, MESH : Wound Infection, MESH: Male, MESH : RNA, Ribosomal, 16S, MESH : Clostridium Infections, MESH: Wounds and Injuries, Clostridium Infections, Wound Infection, Wounds and Injuries, MESH : Bacterial Typing Techniques, MESH : Microbial Sensitivity Tests, MESH : Sequence Analysis, DNA
Abstract

International audience; Misidentification of rare Clostridium species often originated from the environment as clinically relevant species is problematic. A strain isolated from a traumatic leg wound first identified as C. clostridioforme was finally identified as the rare Clostridium celerecrescens. Two similar misidentifications are reported in the literature. In order to help the phenotypic differentiation of C. celerecrescens from the close species of the "C. clostridioforme group", an identification table and differential susceptibilities to 4 selected antibiotics are proposed. Once a clinical isolate is referred to this group, identification should be definitively confirmed by unambiguous methods such as 16s rDNA sequencing.

Published
2012
Full Text
View/download PDF

14. Clostridium difficile O27 colitis: hospital-onset but community-acquired

Author

Michel Cormier, Sylvie Buffet-Bataillon, Pascal Vincent, Pierre Tattevin, H. Sénéchal, Microbiologie : Risques Infectieux, Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-CHU Pontchaillou [Rennes]-Faculté de Chirurgie Dentaire de Rennes-Faculté d'Odontologie-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Service des maladies infectieuses et réanimation médicale [Rennes] = Infectious Disease and Intensive Care [Rennes], CHU Pontchaillou [Rennes], Brébion, Alice, Université de Rennes (UR)-CHU Pontchaillou [Rennes]-Faculté de Chirurgie Dentaire de Rennes-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Université de Rennes (UR)-CHU Pontchaillou [Rennes]-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Université de Rennes - UFR d'Odontologie (UR Odontologie), and Université de Rennes (UR)-Université de Rennes (UR)

Subjects
Male, Antibiotics, 0302 clinical medicine, Medical microbiology, MESH: Aged, 80 and over, MESH: Colitis, Infection control, 030212 general & internal medicine, MESH: Drug Utilization, Epidemic strain, Aged, 80 and over, MESH: Aged, 0303 health sciences, MESH: Middle Aged, General Medicine, Middle Aged, Clostridium difficile, Colitis, University hospital, MESH: Hospitals, Hospitals, Anti-Bacterial Agents, 3. Good health, Community-Acquired Infections, Infectious Diseases, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, MESH: Community-Acquired Infections, Female, France, Adult, Microbiology (medical), medicine.medical_specialty, medicine.drug_class, MESH: Clostridium Infections, 03 medical and health sciences, Internal medicine, MESH: Anti-Bacterial Agents, medicine, Humans, Intensive care medicine, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, MESH: Clostridium difficile, Aged, MESH: Humans, Clostridioides difficile, 030306 microbiology, business.industry, Outbreak, MESH: Adult, medicine.disease, Drug Utilization, MESH: Male, MESH: France, Clostridium Infections, business, MESH: Female
Abstract

International audience; Large outbreaks of Clostridium difficile (CD) associated colitis in North America and Europe have been attributed to the emergence of the epidemic/toxin PCR Ribotype O27 CD strain (CD027). Due to the increased virulence of this epidemic strain and its propension for causing outbreaks, we performed a structured risk-assessment approach in order to determine the risks associated with the introduction of this strain within our university hospital. From February 2009 to January 2010, we identified 31 cases of CD027 associated colitis, whereby twenty one (67.7%) had symptoms onset more than 48 hours after admission and were classified as nosocomial. These patients had received wide-spectrum antimicrobials for other infections in the hospital before CD027 colitis diagnosis. The 31 patients with CD027 were admitted in 20 different units, managed by distinct health-care workers (HCWs), and no contact was identified between patients during their hospital stay. Furthermore, infection control audits showed 100% compliance with institutional guidelines for control of CD colitis. These findings suggest that CD027 is most frequently acquired in the community and emerges sporadically under antibiotic pressure during hospitalization.

Published
2012
Full Text
View/download PDF

15. Rapid diagnosis of Clostridium difficile infection by multiplex real-time PCR.: Diagnosis of Clostridium difficile Infection and Identification of the Epidemic clone 027 by Multiplex Real-Time PCR. (Titre fichier auteur)

Author

Barbut, Frédéric, Monot, Marc, Rousseau, Antoine, Cavelot, Sébastien, Simon, Tabassome, Burghoffer, Béatrice, Lalande, Valérie, Tankovic, Jacques, Petit, Jean-Claude, Dupuy, Bruno, Eckert, C, National reference laboratory for clostridium difficile, Université Pierre et Marie Curie - Paris 6 (UPMC), CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Pathogénèse des Bactéries Anaérobies / Pathogenesis of Bacterial Anaerobes (PBA (U-Pasteur_6)), Institut Pasteur [Paris]-Université Paris Diderot - Paris 7 (UPD7), This study was supported by a grant from the Programme Hospitalier de Recherche Clinique (AOR06006)., Institut Pasteur [Paris] (IP)-Université Paris Diderot - Paris 7 (UPD7), and CHU Saint-Antoine [APHP]

Subjects
MESH: DNA Primers, MESH: Humans, MESH: Multiplex Polymerase Chain Reaction, MESH: Oligonucleotide Probes, MESH: Real-Time Polymerase Chain Reaction, MESH: Clostridium Infections, MESH: Feces, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, MESH: Molecular Diagnostic Techniques, MESH: Bacteriological Techniques, MESH: Sensitivity and Specificity, MESH: Bacterial Toxins, MESH: Repressor Proteins, MESH: Bacterial Proteins, MESH: Clostridium difficile
Abstract

International audience; The gold standards for the diagnosis of Clostridium difficile infections (CDIs) are the cytotoxicity assay and the toxigenic culture. However, both methods are time-consuming and the results are not available before 24-48 h. We developed and evaluated a multiplex in-house real-time polymerase chain reaction (PCR) assay for the simultaneous detection of toxigenic strains of C. difficile and the presumptive identification of the epidemic NAP1/027/BI strain from stools. Amplifications were performed using specific primers for tcdB and tcdC on an ABI Prism 7300 (Applied Biosystems). The detection of amplicons was done using TaqMan probes. The analytical sensitivity of the multiplex real-time PCR for detecting tcdB was estimated to 10 CFU/g of stools. This assay was assessed from 881 consecutive unformed stools from patients suspected of having CDI. The gold standard was the toxigenic culture for the diagnosis of CDI and PCR ribotyping for the identification of the NAP1/027/BI strain. The prevalence of positive toxigenic culture was 9.31%. Compared to the toxigenic culture, the sensitivity, specificity, and positive and negative predictive values were 86.59%, 97.43%, 78.02%, and 98.57%, respectively, for the real-time PCR and 70.73%, 100%, 100%, and 97.08%, respectively, for the cytotoxicity assay.

Published
2011
Full Text
View/download PDF

16. [Collaboration between laboratories with regard to instances of food poisoning]

Author

Vautor, E., Fajardi, V., Elevage des ruminants en régions chaudes (UMR ERRC), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA), DSV 06, Ministère Agriculture, and Vautor, Eric

Subjects
MESH: Clostridium perfringens, MESH: Salmonella Food Poisoning, MESH: Humans, Clostridium perfringens, MESH: Clostridium Infections, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Foodborne Diseases, MESH: France, Interinstitutional Relations, MESH: Public Health Practice, MESH: Food Poisoning, Clostridium Infections, Public Health Practice, MESH: Laboratories, Humans, Salmonella Food Poisoning, France, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Laboratories, MESH: Interinstitutional Relations
Published
2001

Catalog

Books, media, physical & digital resources
See catalog results