1. Two dechlorinated chlordecone derivatives formed by in situ chemical reduction are devoid of genotoxicity and mutagenicity and have lower proangiogenic properties compared to the parent compound
- Author
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Sébastien Bristeau, Sébastien Faure, Samuel Legeay, Pierre-André Billat, Christophe Mouvet, Nicolas Clere, Fabrice Nesslany, Université d'Angers (UA), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP), Impact de l'environnement chimique sur la santé humaine - ULR 4483 (IMPECS), Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Bureau de Recherches Géologiques et Minières (BRGM) (BRGM), and The results presented here were obtained through financing by the French Ministry of Environment, General Directorate for Risk Prevention (action F of the 2015 grant program MEDDE/BRGM).
- Subjects
0301 basic medicine ,Salmonella ,Insecticides ,MESH: Chlordecone/analysis ,Angiogenesis ,Health, Toxicology and Mutagenesis ,MESH: DNA Damage/genetics ,In situ chemical reduction ,MESH: Mutagenicity Tests ,010501 environmental sciences ,medicine.disease_cause ,01 natural sciences ,Mutagenicity ,MESH: Mutagens/toxicity ,Organic chemistry ,Soil Pollutants ,MESH: Mutagenesis ,Chemistry ,Lymphoblast ,MESH: Insecticides/analysis ,MESH: Chlordecone/chemistry ,General Medicine ,respiratory system ,Pollution ,3. Good health ,Chlordecone ,[SDV.TOX]Life Sciences [q-bio]/Toxicology ,congenital, hereditary, and neonatal diseases and abnormalities ,MESH: Soil Pollutants/chemistry ,MESH: Insecticides/chemistry ,03 medical and health sciences ,medicine ,Environmental Chemistry ,Humans ,0105 earth and related environmental sciences ,MESH: Humans ,Mutagenicity Tests ,Neurotoxicity ,medicine.disease ,Molecular biology ,In vitro ,respiratory tract diseases ,030104 developmental biology ,Mutagenesis ,Dechlorination ,Genotoxicity ,Micronucleus ,MESH: Soil Pollutants/analysis ,DNA Damage ,Mutagens - Abstract
International audience; Chlordecone (CLD) is a chlorinated hydrocarbon insecticide, now classified as a persistent organic pollutant. Several studies have previously reported that chronic exposure to CLD leads to hepatotoxicity, neurotoxicity, raises early child development and pregnancy complications, and increases the risk of liver and prostate cancer. In situ chemical reduction (ISCR) has been identified as a possible way for the remediation of soils contaminated by CLD. In the present study, the objectives were (i) to evaluate the genotoxicity and the mutagenicity of two CLD metabolites formed by ISCR, CLD-5a-hydro, or CLD-5-hydro (5a- or 5- according to CAS nomenclature; CLD-1Cl) and tri-hydroCLD (CLD-3Cl), and (ii) to explore the angiogenic properties of these molecules. Mutagenicity and genotoxicity were investigated using the Ames's technique on Salmonella typhimurium and the in vitro micronucleus micromethod with TK6 human lymphoblastoid cells. The proangiogenic properties were evaluated on the in vitro capillary network formation of human primary endothelial cells. Like CLD, the dechlorinated derivatives of CLD studied were devoid of genotoxic and mutagenic activity. In the assay targeting angiogenic properties, significantly lower microvessel lengths formed by endothelial cells were observed for the CLD-3Cl-treated cells compared to the CLD-treated cells for two of the three tested concentrations. These results suggest that dechlorinated CLD derivatives are devoid of mutagenicity and genotoxicity and have lower proangiogenic properties than CLD.
- Published
- 2016