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2 results on '"MESH: Arenaviruses, Old World"'

1. Human Dendritic Cells Infected with the Nonpathogenic Mopeia Virus Induce Stronger T-Cell Responses than Those Infected with Lassa Virus

Author

Sylvain Baize, Marion Russier, Stéphanie Reynard, Delphine Pannetier, Vincent Deubel, Alexandra Journeaux, Noël Tordo, Biologie des Infections Virales Émergentes - Biology of Emerging Viral Infections (UBIVE), Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris] (IP), This work was supported by the Délégation Générale pour l'Armement (0034053). D.P. is a fellow recipient of the Délégation Générale pour l'Armement., Centre International de Recherche en Infectiologie - UMR (CIRI), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris] (IP), and MATEO, MATHIEU

Subjects
CD4-Positive T-Lymphocytes, Enzyme-Linked Immunospot Assay, Cellular immunity, MESH: Flow Cytometry, Lymphocyte Activation, medicine.disease_cause, [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity, 0302 clinical medicine, MESH: Reverse Transcriptase Polymerase Chain Reaction, Cytotoxic T cell, Lassa fever, Cells, Cultured, [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, MESH: Cytokines, 0303 health sciences, MESH: Dendritic Cells, Reverse Transcriptase Polymerase Chain Reaction, MESH: CD4-Positive T-Lymphocytes, MESH: Enzyme-Linked Immunosorbent Assay, MESH: Lassa virus, MESH: Arenaviruses, Old World, Flow Cytometry, 3. Good health, medicine.anatomical_structure, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, MESH: Immunologic Memory, Cytokines, MESH: Cells, Cultured, T cell, Immunology, Enzyme-Linked Immunosorbent Assay, Biology, Microbiology, Virus, MESH: Coculture Techniques, 03 medical and health sciences, MESH: Cell Proliferation, Virology, medicine, Humans, Lassa virus, MESH: Lymphocyte Activation, [SDV.IMM.II] Life Sciences [q-bio]/Immunology/Innate immunity, Cell Proliferation, 030304 developmental biology, MESH: Humans, Arenavirus, Dendritic Cells, Dendritic cell, medicine.disease, biology.organism_classification, Coculture Techniques, Insect Science, Pathogenesis and Immunity, MESH: Enzyme-Linked Immunospot Assay, Arenaviruses, Old World, Immunologic Memory, MESH: T-Lymphocytes, Cytotoxic, T-Lymphocytes, Cytotoxic, 030215 immunology
Abstract

The events leading to death in severe cases of Lassa fever (LF) are unknown. Fatality seems to be linked to high viremia and immunosuppression, and cellular immunity, rather than neutralizing antibodies, appears to be essential for survival. We previously compared Lassa virus (LV) with its genetically close but nonpathogenic homolog Mopeia virus (MV), which was used to model nonfatal LF. We showed that strong and early activation of antigen-presenting cells (APC) may play a crucial role in controlling infection. Here we developed an in vitro model of dendritic-cell (DC)-T-cell coculture in order to characterize human T-cell responses induced by MV- or LV-infected DCs. Our results show very different responses to infection with LV and MV. MV strongly and durably stimulated CD8 + and CD4 + T cells, showing early and high activation, a strong proliferative response, and acquisition of effector and memory phenotypes. Furthermore, robust and functional CD4 + and CD8 + cytotoxic T lymphocytes (CTL) were generated. LV, however, induced only weak memory responses. Thus, this study allows an improved understanding of the pathogenesis and immune mechanisms involved in the control of human LV.

Published
2011
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2. Human Macrophages, but Not Dendritic Cells, Are Activated and Produce Alpha/Beta Interferons in Response to Mopeia Virus Infection

Author

Sylvain Baize, Delphine Pannetier, Caroline Faure, Vincent Deubel, Marie-Claude Georges-Courbot, Biologie des Infections Virales Émergentes - Biology of Emerging Viral Infections (UBIVE), Centre International de Recherche en Infectiologie - UMR (CIRI), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris] (IP)

Subjects
MESH: Membrane Glycoproteins, Apoptosis, Viral Plaque Assay, medicine.disease_cause, Virus Replication, MESH: CD40 Antigens, MESH: Reverse Transcriptase Polymerase Chain Reaction, Lassa fever, MESH: Antigens, CD, Cells, Cultured, 0303 health sciences, Membrane Glycoproteins, MESH: Dendritic Cells, Reverse Transcriptase Polymerase Chain Reaction, MESH: Antigen-Presenting Cells, MESH: Macrophage Activation, MESH: Arenaviruses, Old World, Intercellular Adhesion Molecule-1, 3. Good health, MESH: Viral Plaque Assay, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, B7-1 Antigen, Tumor necrosis factor alpha, MESH: B7-2 Antigen, MESH: Interferon-alpha, MESH: Cells, Cultured, Immunology, Alpha interferon, Antigen-Presenting Cells, HLA-C Antigens, Biology, Microbiology, Virus, 03 medical and health sciences, Antigens, CD, Virology, medicine, Humans, RNA, Messenger, CD40 Antigens, Antigen-presenting cell, MESH: HLA-A Antigens, 030304 developmental biology, MESH: RNA, Messenger, Arenavirus, MESH: Humans, HLA-A Antigens, MESH: Interferon-beta, 030306 microbiology, Interleukin-6, Tumor Necrosis Factor-alpha, Macrophages, MESH: Apoptosis, MESH: Intercellular Adhesion Molecule-1, MESH: Virus Replication, Interferon-alpha, MESH: Macrophages, Dendritic cell, Dendritic Cells, Interferon-beta, Macrophage Activation, medicine.disease, biology.organism_classification, Molecular biology, MESH: Interleukin-6, MESH: HLA-C Antigens, Lassa virus, HLA-B Antigens, Insect Science, MESH: Tumor Necrosis Factor-alpha, MESH: HLA-B Antigens, Pathogenesis and Immunity, MESH: B7-1 Antigen, B7-2 Antigen, Arenaviruses, Old World
Abstract

Lassa virus (LV) and Mopeia virus (MV) are closely related members of the Arenavirus genus, sharing 75% amino acid sequence identity. However, LV causes hemorrhagic fever in humans and nonhuman primates, whereas MV cannot induce disease. We have previously shown that antigen-presenting cells (APC)—macrophages (MP) and dendritic cells (DC)—sustain high replication rates of LV but are not activated, suggesting that they play a role in the immunosuppression observed in severe cases of Lassa fever. Here, we infected human APC with MV and analyzed the cellular responses induced. MV infection was productive in MP and even more so in DC. Apoptosis was not induced in either cell type. Moreover, unlike DC, MP were early and strongly activated in response to MV, as shown by the increased surface expression of CD86, CD80, CD54, CD40, and HLA-abc and by the production of mRNA encoding alpha interferon (IFN-α), IFN-β, tumor necrosis factor alpha and interleukin-6. In addition, MV-infected MP produced less of the virus than DC, which was related to the fact that these cells secreted IFN-α. Thus, the strong activation of MP is probably a major event in the control of MV infection and may be involved in the induction of an adaptive immune response in infected hosts. These results may explain the difference in pathogenicity between LV and MV.

Published
2004
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