Descriptor: "MESH: Antigens, Fungal" - Searchworks@Jio Institute Digital Library Search Results

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17 results on '"MESH: Antigens, Fungal"'

1. Novel mouse monoclonal antibodies specifically recognizing β-(1→3)-D-glucan antigen

Author: Yury E. Tsvetkov, D. V. Yashunsky, Ivan K. Baykov, Yana A. Khlusevich, Alexander A. Karelin, Vadim B. Krylov, Sarah Sze Wah Wong, Vishukumar Aimanianda, Jean-Paul Latgé, Alevtina V. Bardashova, Nina V. Tikunova, Nikolay E. Nifantiev, Andrey L. Matveev, Ljudmila A. Emelyanova, Institute of Chemical Biology and Fundamental Medicine [Novosibirsk, Russia] (ICBFM SB RAS), Siberian Branch of the Russian Academy of Sciences (SB RAS), ND Zelinsky Institute of Organic Chemistry [Moscow, Russia], Novosibirsk State University (NSU), Aspergillus, Institut Pasteur [Paris], Mycologie moléculaire - Molecular Mycology, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Franco-German ANR-DFG (ANR-16-CE92-0031-01 AFUINTERACT to V. Kumar, (http://www.agence-nationale-recherche.fr) and the Franco-Indian CEFIPRA (Project N° 5403-1 to V. Kumar) grants., Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Institut Pasteur [Paris] (IP), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), and ANR-16-CE92-0031,EpiFUS,Rôle de FUS dans la régulation des modifications épigénétiques : conséquences pour la sclérose latérale amyotrophique et la démence frontotemporale(2016)
Subjects: 0301 basic medicine, Antifungal Agents, beta-Glucans, [SDV]Life Sciences [q-bio], MESH: Drug Synergism, Aspergillus fumigatus, MESH: Antibodies, Monoclonal, Mice, Cell Wall, Candida albicans, MESH: Animals, Bovine serum albumin, Fluconazole, MESH: Treatment Outcome, chemistry.chemical_classification, MESH: Microbial Sensitivity Tests, Multidisciplinary, biology, MESH: beta-Glucans, Candidiasis, Antibodies, Monoclonal, Drug Synergism, Oligosaccharide, MESH: Candidiasis, 3. Good health, Treatment Outcome, Biochemistry, Biotinylation, Medicine, MESH: Fluconazole, [SDV.IMM]Life Sciences [q-bio]/Immunology, Drug Therapy, Combination, Female, MESH: Aspergillus fumigatus, Research Article, Antigens, Fungal, medicine.drug_class, Science, 030106 microbiology, Microbial Sensitivity Tests, Monoclonal antibody, 03 medical and health sciences, MESH: Cell Wall, medicine, Animals, Humans, MESH: Mice, MESH: Antigens, Fungal, MESH: Humans, MESH: Candida albicans, [SDV.IMM.IMM]Life Sciences [q-bio]/Immunology/Immunotherapy, biology.organism_classification, MESH: Antifungal Agents, In vitro, Disease Models, Animal, MESH: Drug Therapy, Combination, 030104 developmental biology, chemistry, biology.protein, Hybridoma technology, MESH: Disease Models, Animal, MESH: Female
Abstract: International audience; β-(1→3)-D-Glucan is an essential component of the fungal cell wall. Mouse monoclonal antibodies (mAbs) against synthetic nona-β-(1→3)-D-glucoside conjugated with bovine serum albumin (BSA) were generated using hybridoma technology. The affinity constants of two selected mAbs, 3G11 and 5H5, measured by a surface plasmon resonance biosensor assay using biotinylated nona-β-(1→3)-D-glucan as the ligand, were approximately 11 nM and 1.9 nM, respectively. The glycoarray, which included a series of synthetic oligosaccharide derivatives representing β-glucans with different lengths of oligo-β-(1→3)-D-glucoside chains, demonstrated that linear tri-, penta- and nonaglucoside, as well as a β-(1→6)-branched octasaccharide, were recognized by mAb 5H5. By contrast, only linear oligo-β-(1→3)-D-glucoside chains that were not shorter than pentaglucosides (but not the branched octaglucoside) were ligands for mAb 3G11. Immunolabelling indicated that 3G11 and 5H5 interact with both yeasts and filamentous fungi, including species from Aspergillus, Candida, Penicillium genera and Saccharomyces cerevisiae, but not bacteria. Both mAbs could inhibit the germination of Aspergillus fumigatus conidia during the initial hours and demonstrated synergy with the antifungal fluconazole in killing C. albicans in vitro. In addition, mAbs 3G11 and 5H5 demonstrated protective activity in in vivo experiments, suggesting that these β-glucan-specific mAbs could be useful in combinatorial antifungal therapy.
Published: 2019

2. Exposure to field vs. storage wheat dust: different consequences on respiratory symptoms and immune response among grain workers

Author: Gabriel Reboux, Pascal Wild, Coralie Barrera, Hélène Niculita-Hirzel, Victor Dorribo, Laurence Millon, Peggy Krief, Dessislava Savova-Bianchi, Audrey Laboissière, Brigitta Danuser, Jacques A. Pralong, Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Service de parasitologie et mycologie [CHRU de Besançon], Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Service of Occupational Hygiene (Institute for Work and Health), Institut national de recherche et de sécurité (Vandoeuvre lès Nancy) (INRS ( Vandoeuvre lès Nancy)), and Service of Occupational Medicine (Institute for Work and Health)
Subjects: Male, 0301 basic medicine, Veterinary medicine, Respiratory Tract Diseases, Cryptococcus, MESH: Logistic Models, MESH: Respiratory Function Tests, Immunoglobulin E, MESH: Dust, MESH: Occupational Exposure, MESH: Aerosols, 0302 clinical medicine, Respiratory function, Longitudinal Studies, Respiratory symptoms, Respiratory system, MESH: Longitudinal Studies, Triticum, Sensitization, 2. Zero hunger, MESH: Middle Aged, biology, food and beverages, MESH: Enzyme-Linked Immunosorbent Assay, Dust, Grain workers, Middle Aged, MESH: Interviews as Topic, 030210 environmental & occupational health, Respiratory Function Tests, medicine.anatomical_structure, MESH: Edible Grain, Female, Fungi-specific immunoglobulins, Adult, Aerosols/adverse effects, Agricultural Workers' Diseases/etiology, Agricultural Workers' Diseases/physiopathology, Air Pollutants, Occupational/adverse effects, Antigens, Fungal/blood, Dust/analysis, Edible Grain, Enzyme-Linked Immunosorbent Assay, Fungi, Humans, Interviews as Topic, Logistic Models, Nitric Oxide/analysis, Occupational Exposure/adverse effects, Occupational Exposure/analysis, Respiratory Tract Diseases/etiology, Respiratory Tract Diseases/physiopathology, Switzerland, Triticum/adverse effects, Cattle raisers, Occupational wheat dust exposure, MESH: Fungi, Antigens, Fungal, MESH: Switzerland, MESH: Triticum, Air Pollutants, Occupational, Nitric Oxide, complex mixtures, MESH: Air Pollutants, Occupational, 03 medical and health sciences, Immune system, Occupational Exposure, medicine, MESH: Agricultural Workers' Diseases, MESH: Antigens, Fungal, Aerosols, [SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health, MESH: Humans, Public Health, Environmental and Occupational Health, Total ige, MESH: Adult, biology.organism_classification, MESH: Male, Agricultural Workers' Diseases, respiratory tract diseases, 030104 developmental biology, Wheat dust, MESH: Nitric Oxide, biology.protein, MESH: Respiratory Tract Diseases, MESH: Female
Abstract: The aim of this study was to understand the differential acute effects of two distinct wheat-related dusts, such as field or stored wheat dust handling, on workers' health and how those effects evolved at 6 month intervals. Exposure, work-related symptoms, changes in lung function, and blood samples of 81 workers handling wheat and 61 controls were collected during the high exposure season and 6 months after. Specific IgG, IgE, and precipitins against 12 fungi isolated from wheat dust were titrated by enzyme-linked immunosorbent assay, dissociation-enhanced lanthanide fluorescence immunoassay, and electrosyneresis. The level of fungi was determined in the workers' environment. Levels of exhaled fraction of nitrogen monoxide (F E NO) and total IgE were obtained. Exposure response associations were investigated by mixed logistic and linear regression models. The recent exposure to field wheat dust was associated with a higher prevalence for five of six self-reported airway symptoms and with a lower F E NO than those in the control population. Exposure to stored wheat dust was only associated with cough. No acute impact of exposure on respiratory function was observed. Exposure to field wheat dust led to workers' sensitization against the three field fungi Aureobasidum, Cryptococcus, and Phoma, although exposure to storage wheat dust was associated with tolerance. The level of Ig remained stable 6 months after exposure. The clinical picture of workers exposed to field or storage wheat dust differed. The systematic characterization of the aerosol microbial profile may help to understand the reasons for those differences.
Published: 2018

3. Fungal peptides from pneumonitis hypersensitivity etiologic agents are able to induce specific cellular immune response

Author: Anne-Pauline Bellanger, Laurence Millon, Bénédicte Rognon, Houssein Gbaguidi-Haore, Thibaud Lignon, Christophe Borg, Gabriel Reboux, Yann Godet, Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), Service de parasitologie et mycologie [CHRU de Besançon], Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098) (RIGHT), Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté])-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Service d’Hygiène Hospitalière, Laboratoire Chrono-environnement ( LCE ), Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), Centre Hospitalier Régional Universitaire [Besançon] ( CHRU Besançon ), Service d'Oncologie Médicale [CHRU Besançon], Centre Hospitalier Régional Universitaire [Besançon] ( CHRU Besançon ) -Université de Franche-Comté ( UFC ), Service de parasitologie et mycologie [CHU de Besançon], Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC ( HOTE GREFFON ), and Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Etablissement français du sang [Bourgogne-France-Comté] ( EFS [Bourgogne-France-Comté] ) -Université de Franche-Comté ( UFC )
Subjects: Male, MESH : Aged, Epitope, MESH : Antigens, Fungal, Epitopes, 0302 clinical medicine, MESH: Peptide Fragments, Cells, Cultured, MESH: Middle Aged, ELISPOT, Acquired immune system, Healthy Volunteers, 3. Good health, MESH : Mucorales, MESH: Young Adult, Mucorales, MESH: Mucorales, MESH: Cells, Cultured, Antigens, Fungal, MESH: Epitopes, MESH : Dihydrolipoamide Dehydrogenase, T cell, MESH : Alveolitis, Extrinsic Allergic, Immunology, MESH : Young Adult, MESH : Epitopes, Statistics, Nonparametric, [ SDV.EE.SANT ] Life Sciences [q-bio]/Ecology, environment/Health, Interferon-gamma, 03 medical and health sciences, MESH: Mycobacterium, Humans, MESH : Middle Aged, Aged, Dihydrolipoamide Dehydrogenase, MESH: Antigens, Fungal, MESH: Humans, Histocompatibility Antigens Class I, MESH : Humans, Histocompatibility Antigens Class II, MESH: Adult, Peptide Fragments, MESH : Leukocytes, Mononuclear, 030104 developmental biology, Leukocytes, Mononuclear, MESH: Female, 0301 basic medicine, Enzyme-Linked Immunospot Assay, MESH : Enzyme-Linked Immunospot Assay, MESH: Histocompatibility Antigens Class I, ELISPOT assay, Immunology and Allergy, MESH : Female, Interferon gamma, MESH: Healthy Volunteers, MESH: Aged, Immunity, Cellular, MESH: Statistics, Nonparametric, biology, MESH : Antigens, Bacterial, Middle Aged, MESH : Adult, MESH: Leukocytes, Mononuclear, MESH : Histocompatibility Antigens Class II, medicine.anatomical_structure, Female, MESH : Histocompatibility Antigens Class I, Alveolitis, Extrinsic Allergic, medicine.drug, Adult, MESH: Dihydrolipoamide Dehydrogenase, MESH: Interferon-gamma, MESH : Male, MESH: Alveolitis, Extrinsic Allergic, Major histocompatibility complex, Mycobacterium, MESH: Immunity, Cellular, Young Adult, Immune system, MESH : Cells, Cultured, medicine, MESH : Mycobacterium, MESH : Statistics, Nonparametric, MESH : Interferon-gamma, MESH : Healthy Volunteers, [SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health, Antigens, Bacterial, MESH: Epitope Mapping, MESH : Peptide Fragments, MESH : Immunity, Cellular, biology.organism_classification, MESH: Male, MESH : Epitope Mapping, biology.protein, MESH: Histocompatibility Antigens Class II, Mycobacterium immunogenum, MESH: Enzyme-Linked Immunospot Assay, Epitope Mapping, Hypersensitivity pneumonitis, MESH: Antigens, Bacterial, 030215 immunology, IFNγ
Abstract: International audience; Hypersensitivity pneumonitis (HP) is an immunoallergic disease due to chronic exposure to high quantities of different microorganisms such as Mycobacterium immunogenum (Mi), a mycobacterium, and Lichtheimia corymbifera (Lc), a filamentous fungus. It has recently been demonstrated that the protein DLDH (dihydrolipoyl dehydrogenase), is common to these microorganisms. This study aimed to investigate the immune potential of overlapping peptide pools covering the MiDLDH and LcDLDH.; A selection of 34 peptides, from the MiDLDH and LcDLDH, able to interact with Major Histocompatibility Complex (MHC) 1 and MHC 2, was obtained using three different epitope prediction websites. By means of ELISPOT assays, we compared the frequency of Interferon gamma (IFNγ) secreting peripheral blood mononuclear cells (PBMC) after stimulation with overlapping peptide pools. Tests were performed using cells from 35 healthy blood donors.; One peptide pool containing five peptides from MiDLDH and able to interact with MHC 2 induced a marked IFNγ specific immune response (Pool F, p
Published: 2017

4. Surveillance and management of Echinococcus multilocularis in a wildlife park

Author: Jean-Marc Boucher, Frédéric Grenouillet, Amandine Guenon, Damien Montange, Vanessa Hormaz, Franck Boué, Jennifer Lahoreau, Gérald Umhang, Agence Nationale de Sécurité Sanitaire, de l'Alimentation, de l'environnement et du Travail ( ANSES ), ANSES, Laboratoire Génie des procédés papetiers ( LGP2 ), Centre Technique du Papier ( CTP ) -Institut National Polytechnique de Grenoble ( INPG ) -École Française de Papeterie et des Industries Graphiques-Centre National de la Recherche Scientifique ( CNRS ), Laboratoire Chrono-environnement ( LCE ), Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Laboratoire Génie des procédés papetiers (LGP2 ), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut National Polytechnique de Grenoble (INPG)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), and Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)
Subjects: Male, 0301 basic medicine, Veterinary medicine, Foxes, Captivity, Lemur catta, Serology, Feces, MESH : Antigens, Fungal, 0302 clinical medicine, Parasite hosting, MESH : Female, MESH : Environmental Monitoring, biology, Arvicolinae, 030108 mycology & parasitology, MESH : Adult, 3. Good health, Intestines, MESH : Air Pollution, Indoor, Infectious Diseases, Liver, Epidemiological Monitoring, MESH: Inhalation Exposure, MESH: Aspergillus fumigatus, MESH: Environmental Monitoring, medicine.drug, Echinococcosis, Hepatic, MESH: Cystic Fibrosis, MESH : Male, 030231 tropical medicine, Animals, Wild, Rodentia, Echinococcus multilocularis, Albendazole, [ SDV.EE.SANT ] Life Sciences [q-bio]/Ecology, environment/Health, 03 medical and health sciences, Echinococcosis, MESH : Cystic Fibrosis, parasitic diseases, medicine, Animals, MESH : France, MESH: Antigens, Fungal, [SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health, MESH: Humans, Lemur, MESH : Humans, MESH : Aspergillosis, Allergic Bronchopulmonary, MESH: Adult, biology.organism_classification, MESH: Male, MESH: DNA, Fungal, MESH: France, Metacestode, MESH: Aspergillosis, Allergic Bronchopulmonary, MESH : Aspergillus fumigatus, Parasitology, MESH : Inhalation Exposure, MESH: Air Pollution, Indoor, MESH : DNA, Fungal, MESH: Female
Abstract: International audience; The fox tapeworm Echinococcus multilocularis is the causative agent of alveolar echinococcosis, a severe zoonotic disease that may be fatal if untreated. A broad spectrum of mammalian species may be accidentally infected even in captivity. In April 2011, liver lesions due to E. multilocularis were observed during the necropsy of a captive-born nutria (Myocastor coypus) in a French wildlife park, leading to initiation of a study to survey the parasite's presence in the park. A comparable environmental contamination with fox's feces infected by E. multilocularis was reported inside (17.8%) and outside (20.6%) the park. E. multilocularis worms were found in the intestines of three of the five roaming foxes shot in the park. Coprological analyses of potential definitive hosts in captivity (fox, lynx, wildcat, genet, wolf, bear and raccoon) revealed infection in one Eurasian wolf. Voles trapped inside the park also had a high prevalence of 5.3%. After diagnosis of alveolar echinococcosis in a Lemur catta during necropsy, four other cases in L. catta were detected by a combination of ultrasound and serology. These animals were treated twice daily with albendazole. The systematic massive metacestode development and numerous protoscoleces in L. catta confirmed their particular sensitivity to E. multilocularis infection. The autochthonous origin of the infection in all the captive animals infected was genetically confirmed by EmsB microsatellite analysis. Preventive measures were implemented to avoid the presence of roaming foxes, contact with potential definitive hosts and contaminated food sources for potential intermediate hosts.
Published: 2016

5. Respiratory health effects of fifteen years of improved collective protection in a wheat-processing worker population

Author: Pascal Wild, Hélène Niculita-Hirzel, Peggy Krief, Brigitta Danuser, Victor Dorribo, Jacques A. Pralong, Gabriel Reboux, Direction Scientifique, Pôle Trajectoires - Rouen Business School ( Tr@jectoires ), Rouen Business School, Laboratoire Chrono-environnement ( LCE ), Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), Department of Ecology & Evolution, University of Lausanne, Pôle Trajectoires - Rouen Business School (Tr@jectoires), Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Department of Ecology and Evolution [Lausanne], Université de Lausanne (UNIL), and Millon, Laurence
Subjects: Male, MESH : Molecular Weight, occupational wheat dust exposure, Respiratory Tract Diseases, agricultural workers’ lung diseases, MESH : Aged, Cumulative Exposure, MESH : Blotting, Western, Toxicology, MESH : Antigens, Fungal, 0302 clinical medicine, MESH : Farmer's Lung, MESH : Antibodies, Fungal, dose-effect correlation model, Respiratory system, Waste Management and Disposal, Triticum, lcsh:Environmental sciences, Nose, lcsh:GE1-350, 2. Zero hunger, MESH: Aged, MESH: Immunoglobulin G, education.field_of_study, Farmers, MESH: Middle Aged, MESH: Molecular Weight, food and beverages, Dust, Middle Aged, respiratory system, MESH : Adult, 030210 environmental & occupational health, MESH: Predictive Value of Tests, MESH : Serologic Tests, medicine.anatomical_structure, MESH : Mucorales, MESH: Mucorales, Female, Occupational exposure, MESH : Sensitivity and Specificity, Adult, Dust/analysis, Dust/prevention & control, Edible Grain, Humans, Occupational Exposure, Personal Protective Equipment/utilization, Respiratory Tract Diseases/chemically induced, Respiratory Tract Diseases/epidemiology, Switzerland, MESH : Immunoglobulin G, Population, [ SDV.EE.SANT ] Life Sciences [q-bio]/Ecology, environment/Health, lcsh:Agriculture, 03 medical and health sciences, FEV1/FVC ratio, medicine, [SDV.EE.SANT] Life Sciences [q-bio]/Ecology, environment/Health, MESH: Blotting, Western, MESH : Middle Aged, MESH : Predictive Value of Tests, education, Personal Protective Equipment, Ecology, Evolution, Behavior and Systematics, Respiratory health, MESH: Antigens, Fungal, MESH: Farmer's Lung, [SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health, collective protective equipment, Lung, MESH: Humans, business.industry, MESH: Serologic Tests, MESH: Antibodies, Fungal, MESH : Humans, lcsh:S, Public Health, Environmental and Occupational Health, MESH: Adult, MESH: Sensitivity and Specificity, respiratory tract diseases, 030228 respiratory system, business
Abstract: Introduction Occupational exposure to grain dust causes respiratory symptoms and pathologies. To decrease these effects, major changes have occurred in the grain processing industry in the last twenty years. However, there are no data on the effects of these changes on workers’ respiratory health. Objectives The aim of this study was to evaluate the respiratory health of grain workers and farmers involved in different steps of the processing industry of wheat, the most frequently used cereal in Europe, fifteen years after major improvements in collective protective equipment due to mechanisation. Material and Methods Information on estimated personal exposure to wheat dust was collected from 87 workers exposed to wheat dust and from 62 controls. Lung function (FEV 1 , FVC, and PEF), exhaled nitrogen monoxide (F E NO) and respiratory symptoms were assessed after the period of highest exposure to wheat during the year. Linear regression models were used to explore the associations between exposure indices and respiratory effects. Results Acute symptoms – cough, sneezing, runny nose, scratchy throat – were significantly more frequent in exposed workers than in controls. Increased mean exposure level, increased cumulative exposure and chronic exposure to more than 6 mg.m -3 of inhaled wheat dust were significantly associated with decreased spirometric parameters, including FEV 1 and PEF (40 ml and 123 ml.s -1 ), FEV 1 and FVC (0.4 ml and 0.5 ml per 100 h.mg.m -3 ), FEV 1 and FVC (20 ml and 20 ml per 100 h at >6 mg.m -3 ). However, no increase in F E NO was associated with increased exposure indices. Conclusions The lung functions of wheat-related workers are still affected by their cumulative exposure to wheat dust, despite improvements in the use of collective protective equipment.
Published: 2015

6. Serum Aspergillus Galactomannan for the Management of Disseminated Histoplasmosis in AIDS

Author: Blandine Denis, Fanny Lanternier, Marie-Elisabeth Bougnoux, Olivier Lortholary, Sébastien Rivière, Marc Lecuit, Service des Maladies infectieuses et tropicales [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Laboratoire de Microbiologie [AP-HP Necker], Université Paris Descartes - Paris 5 (UPD5)-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université Paris Descartes - Paris 5 (UPD5), Centre National de Référence Mycologie et Antifongiques-Mycologie Moléculaire (CNRMA), Institut Pasteur [Paris], and Institut Pasteur [Paris] (IP)
Subjects: Antifungal Agents, [SDV]Life Sciences [q-bio], Serology, Mannans, chemistry.chemical_compound, 0302 clinical medicine, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, MESH: Histoplasmosis, 030212 general & internal medicine, Histoplasmosis, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, 0303 health sciences, biology, MESH: AIDS-Related Opportunistic Infections, MESH: HIV, Articles, 3. Good health, Aspergillus, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Infectious Diseases, MESH: Aspergillus, Female, Adult, Antigens, Fungal, Isolation (health care), AIDS-Related Opportunistic Infections, Histoplasma, Context (language use), 03 medical and health sciences, Galactomannan, MESH: Mannans, Antigen, Virology, medicine, Humans, MESH: Histoplasma, MESH: Antigens, Fungal, MESH: Acquired Immunodeficiency Syndrome, Acquired Immunodeficiency Syndrome, MESH: Humans, 030306 microbiology, Galactose, HIV, MESH: Adult, MESH: Antifungal Agents, biology.organism_classification, medicine.disease, chemistry, Immunology, Parasitology, MESH: Female, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: International audience; Disseminated histoplasmosis is an emerging infection in patients with cellular immune deficiency in non-endemic countries, caused by the migration from endemic regions and the development of travels. Diagnosis can be challenging in this context because rapid diagnostic tools such as Histoplasma antigen detection or appropriate molecular tools are generally unavailable, serology is often negative in immunosuppressed patients, and isolation of the fungus from cultures often takes several weeks. Here, we report the contribution of galactomannan serum detection for the management of an HIV-infected patient with disseminated histoplasmosis.
Published: 2012

7. Diagnosis, management and outcome of Candida endocarditis

Author: A. Lefort, Arnaud Fontanet, Isabelle Podglajen, Stéphane Bretagne, Mary S. Wolff, J.-L. Mainardi, Boualem Sendid, Marie Desnos-Ollivier, Loïc Chartier, Olivier Lortholary, Service des Maladies infectieuses et tropicales [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut Pasteur [Paris] (IP), Université Lille Nord (France), Université Paris Diderot - Paris 7 (UPD7), AP-HP - Hôpital Bichat - Claude Bernard [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université Paris Descartes - Paris 5 (UPD5), Université Pierre et Marie Curie - Paris 6 (UPMC), Centre de Recherche des Cordeliers (CRC (UMR_S 872)), Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Centre National de Référence des Mycoses invasives et antifongiques - Mycologie moléculaire (CNRMA), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Epidémiologie des Maladies Emergentes - Emerging Diseases Epidemiology, Pasteur-Cnam Risques infectieux et émergents (PACRI), Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM), Hôpital Henri Mondor, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), This study received grants from Pfizer and Merck Sharp & Dohme-Chibret, France. The sponsors had no role in designing or conducting the study, the collection, management, analysis and interpretation of the data, or the preparation, review or approval of the manuscript. Financial disclosure: O. Lortholary has received research grants from Pfizer and Gilead Sciences, is a consultant for Gilead Sciencesand Astellas, and is a member of the speaker’s bureaus of Pfizer, Merck, Astellas, Gilead Sciences, and Schering Plough.J. L. Mainardi has received a research grant from Novartis. S. Bretagne is a consultant for Gilead Sciences, and hasreceived speaking honoraria from Pfizer and Gilead Sciences and travel grants from Astellas, Pfizer, and Schering-Plough.M. Wolff has received consulting and lecture fees from Astellas, Gilead, Janssen-Cilag, and Aventis., The authors thank the Direction Médicale de l'Institut Pasteur, Paris, for having promoted the study, and A. Desjardins for her careful review of the manuscript. O. Lortholary had full access to all of the data in the study, and takes responsibility for the integrity of the data and the accuracy of the data analysis., BOUYSSIE, Reine, Institut Pasteur [Paris], Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM), and HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM)
Subjects: Male, MESH: Endocarditis, Antifungal Agents, beta-Glucans, medicine.medical_treatment, Kaplan-Meier Estimate, 0302 clinical medicine, antibody detection, MESH: Aged, 80 and over, Valve replacement, Risk Factors, MESH: Risk Factors, MESH: Child, Blood culture, Prospective Studies, 030212 general & internal medicine, Young adult, Child, DNA, Fungal, Substance Abuse, Intravenous, Prospective cohort study, Fluconazole, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, Candida, MESH: Treatment Outcome, Aged, 80 and over, MESH: Aged, 0303 health sciences, Univariate analysis, MESH: Microbial Sensitivity Tests, MESH: Middle Aged, medicine.diagnostic_test, MESH: beta-Glucans, Age Factors, Candidiasis, Fungal genetics, General Medicine, MESH: Follow-Up Studies, Middle Aged, MESH: Candidiasis, 3. Good health, Treatment Outcome, Infectious Diseases, MESH: Young Adult, Aortic Valve, endocarditis, MESH: Fluconazole, Female, Proteoglycans, Adult, Microbiology (medical), medicine.medical_specialty, Antigens, Fungal, Adolescent, Microbial Sensitivity Tests, Lower risk, molecular tools, Young Adult, 03 medical and health sciences, MESH: Candida, Internal medicine, medicine, Humans, Endocarditis, Antibodies, Fungal, MESH: Kaplan-Meier Estimate, Aged, MESH: Antigens, Fungal, MESH: Adolescent, MESH: Age Factors, MESH: Humans, 030306 microbiology, business.industry, MESH: Antibodies, Fungal, MESH: Adult, medicine.disease, antigen detection, MESH: Antifungal Agents, [SDV.MP.MYC] Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, MESH: Male, MESH: Prospective Studies, Surgery, MESH: DNA, Fungal, MESH: Substance Abuse, Intravenous, business, MESH: Aortic Valve, MESH: Female, Follow-Up Studies
Abstract: International audience; Limited data exist on Candida endocarditis (CE) outcome in the era of new antifungals. As early diagnosis of CE remains difficult, non-culture-based tools need to be evaluated. Through the French prospective MYCENDO study (2005-2007), the overall characteristics and risk factors for death from CE were analysed. The contribution of antigen detection (mannan/anti-mannan antibodies and (1,3)-β-d-glucans) and molecular tools was evaluated. Among 30 CE cases, 19 were caused by non-albicans species. Sixteen patients (53%) had a predisposing cardiac disease, which was a valvular prosthesis in ten (33%). Nine patients (30%) were intravenous drug users; none of them had right-sided CE. Among the 21 patients who were not intravenous drug users, 18 (86%) had healthcare-associated CE. Initial therapy consisted of a combination of antifungals in 12 of 30 patients (40%). Thirteen patients (43%) underwent valve replacement. The median follow-up was 1 year after discharge from hospital (range, 5 months to 4 years) and hospital mortality was 37%. On univariate analysis, patients aged ≥60 years had a higher mortality risk (OR 11, 95% CI 1.2-103.9; p 0.024), whereas intravenous drug use was associated with a lower risk of death (OR 0.12, 95% CI 0.02-0.7; p 0.03). Among 18 patients screened for both serum mannan/anti-mannan antibodies and (1,3)-β-d-glucans, all had a positive result with at least one of either test at CE diagnosis. Real-time PCR was performed on blood (SeptiFast) in 12 of 18, and this confirmed the blood culture results. In conclusion, CE prognosis remains poor, with a better outcome among younger patients and intravenous drug users. Detection of serum antigens and molecular tools may contribute to earlier CE diagnosis.
Published: 2012

8. Prospective evaluation of clinical and biological markers to predict the outcome of invasive pulmonary aspergillosis in hematological patients

Author: Jean Menotti, Karine Chagnon, Muriel Cornet, Claire Lacroix, Jean Louis Poirot, Sophie Touratier, Emmanuel Raffoux, Jean-Paul Latgé, Benoit Brethon, Abdellatif Tazi, Françoise Isnard, Francis Derouin, Patricia Ribaud, Claire Bouges-Michel, Annie Sulahian, Raphaël Porcher, Anne Vekhoff, Anne Bergeron, Equipe 5 : METHODS - Méthodes de l’évaluation thérapeutique des maladies chroniques (CRESS - U1153), Université Paris Descartes - Paris 5 (UPD5)-Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA), Université Paris Descartes - Paris 5 (UPD5), Service de réanimation médicale, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Diderot - Paris 7 (UPD7)-Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Thérapeutique Recombinante Expérimentale (TIMC-IMAG-TheREx), Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 (TIMC-IMAG), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), Service d'hématologie biologique, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Pharmacie, Hôpital Saint-Louis, Aspergillus, Institut Pasteur [Paris], Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA), Service de pneumologie [Saint-Louis], Université Paris Diderot - Paris 7 (UPD7)-Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Assistance Publique Hôpitaux de Paris et Faculté Denis Didero, Laboratoire de Parasitologie-Mycologie, Hopital Saint-Louis [AP-HP] (AP-HP), Service d'hématologie greffe [Saint-Louis], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Diderot - Paris 7 (UPD7), Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP), CHU Saint-Antoine [APHP], Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP)-IMAG-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP)-IMAG-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Département d’Immunologie Biologique [AP-HP - Hôpital Saint-Antoine, Paris], Unité d’autoimmunité, AP-HP - Hôpital Saint-Antoine -AP-HP - Hôpital Saint-Antoine, Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (APHP), Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Saint Louis [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Université Paris Diderot - Paris 7 (UPD7)-Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Université Paris Diderot - Paris 7 (UPD7), Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP), Equipe 5 : METHODS - Méthodes de l’évaluation thérapeutique des maladies chroniques ( CRESS - U1153 ), Université Paris Descartes - Paris 5 ( UPD5 ) -Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité ( CRESS (U1153 / UMR_A 1125) ), Institut National de la Recherche Agronomique ( INRA ) -Université Sorbonne Paris Cité ( USPC ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Institut National de la Recherche Agronomique ( INRA ) -Université Sorbonne Paris Cité ( USPC ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Université Paris Descartes - Paris 5 ( UPD5 ), Assistance publique - Hôpitaux de Paris (AP-HP)-Université Paris Diderot - Paris 7 ( UPD7 ) -Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Thérapeutique Recombinante Expérimentale ( TIMC-IMAG-THEREX ), Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications [Grenoble] ( TIMC-IMAG ), Université Joseph Fourier - Grenoble 1 ( UJF ) -Institut polytechnique de Grenoble - Grenoble Institute of Technology ( Grenoble INP ) -IMAG-Centre National de la Recherche Scientifique ( CNRS ) -Université Grenoble Alpes ( UGA ) -Université Joseph Fourier - Grenoble 1 ( UJF ) -Institut polytechnique de Grenoble - Grenoble Institute of Technology ( Grenoble INP ) -IMAG-Centre National de la Recherche Scientifique ( CNRS ) -Université Grenoble Alpes ( UGA ), Assistance publique - Hôpitaux de Paris (AP-HP)-Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Assistance publique - Hôpitaux de Paris - AP-HP (FRANCE), Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité ( CRESS (U1153 / UMR_A 1125) ), Institut National de la Recherche Agronomique ( INRA ) -Université Sorbonne Paris Cité ( USPC ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), and Assistance publique - Hôpitaux de Paris (AP-HP)-Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris]-Université Paris Diderot - Paris 7 ( UPD7 )
Subjects: Male, Antifungal Agents, MESH : Prospective Studies, MESH : Aged, Aspergillosis, Mannans, MESH : Antigens, Fungal, chemistry.chemical_compound, 0302 clinical medicine, MESH : Child, MESH: Child, MESH : Female, Longitudinal Studies, Prospective Studies, MESH : Biomarkers, 030212 general & internal medicine, Young adult, Child, MESH : Immunosuppressive Agents, Prospective cohort study, MESH: Longitudinal Studies, [ SDV.MP.MYC ] Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, MESH: Treatment Outcome, Invasive Pulmonary Aspergillosis, MESH : Longitudinal Studies, MESH: Aged, 0303 health sciences, MESH : Prognosis, MESH: Middle Aged, Respiratory disease, Middle Aged, MESH : Adult, Prognosis, 3. Good health, Treatment Outcome, MESH: Young Adult, MESH: Survival Analysis, Female, Steroids, MESH: Immunosuppressive Agents, Immunosuppressive Agents, Adult, Microbiology (medical), medicine.medical_specialty, MESH: Invasive Pulmonary Aspergillosis, Antigens, Fungal, Adolescent, MESH : Male, MESH : Antifungal Agents, MESH : Young Adult, Mycology, MESH : Treatment Outcome, MESH: Prognosis, Young Adult, 03 medical and health sciences, Galactomannan, MESH: Mannans, MESH : Adolescent, Internal medicine, medicine, Humans, MESH : Middle Aged, Mycosis, Survival analysis, Aged, MESH: Antigens, Fungal, MESH: Adolescent, MESH: Humans, 030306 microbiology, business.industry, MESH : Humans, Galactose, Cancer, MESH: Adult, medicine.disease, MESH: Antifungal Agents, Survival Analysis, MESH: Male, MESH: Prospective Studies, MESH: Steroids, Surgery, chemistry, MESH: Biomarkers, MESH : Invasive Pulmonary Aspergillosis, MESH : Survival Analysis, business, MESH : Mannans, MESH: Female, Biomarkers, MESH : Steroids
Abstract: Early evaluation of treatment efficacy in invasive aspergillosis (IA), a leading cause of morbidity and mortality in hematological patients, remains a challenge. We conducted a prospective study to evaluate the performance of different markers in predicting the outcome of patients with IA. Both clinical and biological criteria were assessed 7, 14, 21, and 45 days after inclusion in the study, and mortality was assessed at day 60. The association between baseline data and their evolution and the day 45 response to treatment was analyzed. A total of 57 patients (4 with proven, 44 with probable, and 9 with possible aspergillosis according to the revised EORTC/MSG [European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and National Institute of Allergy and Infectious Diseases Mycoses Study Group] definitions) were included. At day 45, 30 patients (53%) were determined to be responders, 25 (44%) were nonresponders, and 2 were not able to be evaluated. Twenty patients died within the 60 days of follow-up. We found that a poor day 45 outcome was associated with patients who had high baseline serum galactomannan (GM) antigen levels and those receiving steroids at the time of IA. A consistently negative serum GM index was associated with a good outcome, and the day 14 clinical evaluation was predictive of the day 45 outcome. No association was found between Aspergillus antibodies or DNA detection and patients' outcome. We conclude that the GM index value at diagnosis of IA, GM index kinetics, and clinical evaluation at day 14 are good markers for predicting the outcome of patients with IA and should be taken into account for adapting antifungal treatment.
Published: 2012

9. Anti-inflammatory effect of fluvastatin on IL-8 production induced by Pseudomonas aeruginosa and Aspergillus fumigatus antigens in cystic fibrosis

Author: G. Brinchault, Benoit Desrues, Jeanne Galaine, Mélanie Bonizec, Chantal Belleguic, Stéphane Jouneau, Corinne Martin-Chouly, Jean-Pierre Gangneux, Service de pneumologie, Signalisation et Réponses aux Agents Infectieux et Chimiques (SeRAIC), Université de Rennes (UR), Service de Parasitologie-Mycologie [Rennes], Université de Rennes (UR)-Hôpital Pontchaillou-CHU Pontchaillou [Rennes], 'Vaincre la mucoviscidose', le Conseil scientifique de la Faculté de Médecine de l'Université de Rennes 1, Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), and Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Hôpital Pontchaillou-CHU Pontchaillou [Rennes]
Subjects: Male, Pulmonology, Pharmacology, MESH: Terpenes, Aspergillus fumigatus, 0302 clinical medicine, MESH: Indoles, 0303 health sciences, Clinical Pharmacology, MESH: Case-Control Studies, 3. Good health, MESH: Young Adult, [SDV.TOX]Life Sciences [q-bio]/Toxicology, Cytokines, Medicine, Antigens, Fungal, Science, Immunology, Mevalonic Acid, Mevalonic acid, Microbiology, 03 medical and health sciences, Inhibitory Concentration 50, Genetics, Humans, Aspergillosis, Pseudomonas Infections, Biology, MESH: Antigens, Fungal, MESH: Adolescent, MESH: Humans, Immunity, MESH: Adult, Molecular Development, MESH: Interleukin-8, 030228 respiratory system, chemistry, Pharmacodynamics, Case-Control Studies, MESH: Anti-Inflammatory Agents, Mutation, MESH: Lipopolysaccharides, MESH: Female, Developmental Biology, Lipopolysaccharides, Bacterial Diseases, Chemokine, Indoles, Anatomy and Physiology, Cystic Fibrosis, Anti-Inflammatory Agents, Cystic Fibrosis Transmembrane Conductance Regulator, medicine.disease_cause, Fatty Acids, Monounsaturated, chemistry.chemical_compound, Autosomal Recessive, Immune Physiology, Whole blood, MESH: Inhibitory Concentration 50, MESH: Cystic Fibrosis Transmembrane Conductance Regulator, Multidisciplinary, biology, Fungal Diseases, MESH: Mevalonic Acid, MESH: Fatty Acids, Monounsaturated, Infectious Diseases, Pseudomonas aeruginosa, MESH: Pseudomonas aeruginosa, Female, medicine.symptom, MESH: Aspergillus fumigatus, medicine.drug, Research Article, Adult, Drugs and Devices, MESH: Mutation, Adolescent, MESH: Cystic Fibrosis, Clinical Research Design, Preclinical Models, Inflammation, Young Adult, medicine, Interleukin 8, Fluvastatin, 030304 developmental biology, Clinical Genetics, Terpenes, Interleukin-8, Human Genetics, biology.organism_classification, MESH: Male, Immune System, biology.protein, Clinical Immunology
Abstract: International audience; BACKGROUND: Early in life, patients with cystic fibrosis (CF) are infected with microorganisms including bacteria and fungi, particularly Pseudomonas aeruginosa and Aspergillus fumigatus. Since recent research has identified the anti-inflammatory properties of statins (besides their lipid-lowering effects), we investigated the effect of fluvastatin on the production of the potent neutrophil chemoattractant chemokine, IL-8, in whole blood from CF patients, stimulated by Pseudomonas aeruginosa (LPS) and Aspergillus fumigatus (AFA) antigens. RESULTS: Whole blood from adult patients with CF and from healthy volunteers was collected at the Rennes University Hospital (France). Blood was pretreated for 1 h with fluvastatin (0-300 µM) and incubated for 24 h with LPS (10 µg/mL) and/or AFA (diluted 1/200). IL-8 protein levels, quantified by ELISA, were increased in a concentration-dependent manner when cells were stimulated by LPS or AFA. Fluvastatin strongly decreased the levels of IL-8, in a concentration-dependent manner, in whole blood from CF patients. However, its inhibitory effect was decreased or absent in whole blood from healthy subjects. Furthermore, the inhibition induced by fluvastatin in CF whole blood was reversed in the presence of intermediates within the cholesterol biosynthesis pathway, mevalonate, farnesyl pyprophosphate or geranylgeranyl pyrophosphate that activate small GTPases by isoprenylation. CONCLUSIONS: For the first time, the inhibitory effects of fluvastatin on CF systemic inflammation may reveal the important therapeutic potential of statins in pathological conditions associated with the over-production of pro-inflammatory cytokines and chemokines as observed during the manifestation of CF. The anti-inflammatory effect could be related to the modulation of the prenylation of signalling proteins.
Published: 2011

10. Effects of age, gender and time on receptor expression and anti-Aspergillus functions of human phagocytes

Author: Hervé Pelloux, C. Rolland, Jean Boutonnat, Gaelle Bal, Jean Paul Brion, Magali Hypolite, Renée Grillot, Chantal Dumestre-Pérard, Rose-Laurence Bertini, Delphine Aldebert, Laboratoire Adaptation et pathogénie des micro-organismes [Grenoble] (LAPM), Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), Université Grenoble Alpes - UFR Médecine (UGA UFRM), Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Techniques pour l'Evaluation et la Modélisation des Actions de la Santé (TIMC-IMAG-ThEMAS), Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 (TIMC-IMAG), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), Biologie du Cancer et de l'Infection (BCI ), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Laboratoire de parasitologie-mycologie, CHU Grenoble, Maladie Infectieuse, Service de Médecine Interne et Maladies Infectieuses, Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS), and Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Male, Receptor expression, MESH: Phagocytes, 0302 clinical medicine, Receptor, MESH: Phagocytosis, MESH: Antigens, CD, Immunologic Surveillance, Immune adherence reaction, 0303 health sciences, Phagocytes, Sex Characteristics, MESH: Middle Aged, Age Factors, MESH: Reactive Oxygen Species, Middle Aged, 3. Good health, Respiratory burst, medicine.anatomical_structure, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, MESH: Young Adult, MESH: Immunity, Innate, Female, MESH: Aspergillus fumigatus, MESH: Sex Characteristics, Adult, Antigens, Fungal, CD14, Immunology, Biology, Granulocyte, In Vitro Techniques, 03 medical and health sciences, Young Adult, Immune system, Phagocytosis, Antigens, CD, medicine, Humans, 030304 developmental biology, MESH: Antigens, Fungal, MESH: Immunologic Surveillance, MESH: Age Factors, Innate immune system, MESH: Humans, Aspergillus fumigatus, MESH: Adult, MESH: Male, Immunity, Innate, Reactive Oxygen Species, MESH: Female, 030215 immunology
Abstract: International audience; Phagocytes play a central role in immune defense. Their dysfunction predisposes to infections. This study determined the expression level of nine receptors involved in Aspergillus immune response as well as the values of phagocytosis and production of radical oxygen species after Aspergillus stimulation, in a healthy adult population. The expression values of the CD11b, CD11c, CD14, CD18, CD35, CD181, CD182, CD282 and CD284 receptors on peripheral human monocytes and granulocytes was established. A heterogenous expression of the CD282 on granulocytes was observed as CD181, CD182 and CD284 on monocytes. Similarly, we observed considerable variation in the expression of these receptors over time. Only CD282 on granulocytes varied with sex. No variation with age was observed. Adherence of Aspergillus conidia to phagocytes was dependent of individual, sex, age and time. A better characterization of these innate immunity parameters is necessary to develop in the future an immunologic surveillance strategy for transplant recipients.
Published: 2010

11. The use of mannan antigen and anti-mannan antibodies in the diagnosis of invasive candidiasis: recommendations from the Third European Conference on Infections in Leukemia

Author: Stéphane Bretagne, Maurizio Sanguinetti, Paul Verweij, Janos Sinko, MALGORZATA MIKULSKA, Nikolay Klimko, Thierry Calandra, Frederic Lamoth, Malcolm Richardson, Daniel Poulain, Maiken Cavling Arendrup, Division of Infectious Diseases, San Martino University Hospital, nfectious Diseases Service, Université de Lausanne (UNIL)-Centre Hospitalier Universitaire Vaudois [Lausanne] (CHUV), Istituto di Microbiologia - Institute of Microbiology [Rome], Università cattolica del Sacro Cuore [Piacenza e Cremona] (Unicatt), Physiopathologie des Candidoses, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, Droit et Santé, University of Genoa (UNIGE)-San Martino University Hospital, the Third European Conference on Infections in Leukemia Group, Université de Lausanne = University of Lausanne (UNIL)-Centre Hospitalier Universitaire Vaudois [Lausanne] (CHUV), Università degli studi di Genova = University of Genoa (UniGe)-San Martino University Hospital, BMC, Ed., Third European Conference on Infections in Leukemia Group, Akova, M., Arendrup, M., Barnes, R., Bille, J., Bretagne, S., Calandra, T., Castagnola, E., Cordonnier, C., Cornely, OA., Cruciani, M., Cuenca-Estrella, M., Dannaoui, E., De La Camara, R., Dellow, E., Donnelly, P., Drgona, L., Einsele, H., Engelhard, D., Flückiger, U., Gachot, B., Gonzales-Moreno, J., Groll, A., Hanel, I., Herbrecht, R., Heussel, CP., Jones, B., Kibbler, C., Klimko, N., Klingspor, L., Kouba, M., Lamoth, F., Lanternier, F., Lehrnbecher, T., Loeffler, J., Lortholary, O., Maertens, J., Marchetti, O., Maschan, A., Mikulska, M., Pagano, L., Petrikos, G., Poulain, D., Racil, Z., Reusser, P., Ribaud, P., Richardson, M., Rizzi-Puechal, V., Ruhnke, M., Sanguinetti, M., Sinko, J., Skiada, A., Styczynski, J., Thiebaut, A., Verweij, P., Viscoli, C., Wahl, J., Ward, K., and White, P.
Subjects: [SDV.MHEP.PHY] Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Critical Care and Intensive Care Medicine, Gastroenterology, Serology, law.invention, Mannans, 0302 clinical medicine, MESH: Practice Guidelines as Topic, law, Medicine, MESH: Animals, 030212 general & internal medicine, Candida, Randomized Controlled Trials as Topic, Mannan, 0303 health sciences, Leukemia, mannan, Intensive care unit, 3. Good health, Europe, Practice Guidelines as Topic, medicine.medical_specialty, Antigens, Fungal, Settore MED/07 - MICROBIOLOGIA E MICROBIOLOGIA CLINICA, Animals, Antibodies, Fungal/biosynthesis, Antigens, Fungal/immunology, Candida/immunology, Candidiasis, Invasive/diagnosis, Candidiasis, Invasive/immunology, Congresses as Topic, Humans, Leukemia/complications, Leukemia/immunology, Mannans/immunology, Practice Guidelines as Topic/standards, Randomized Controlled Trials as Topic/standards, 03 medical and health sciences, MESH: Mannans, MESH: Candida, Internal medicine, MESH: Leukemia, [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Candidiasis, Invasive, Antibodies, Fungal, MESH: Antigens, Fungal, MESH: Humans, 030306 microbiology, business.industry, Research, MESH: Antibodies, Fungal, Cancer, Odds ratio, medicine.disease, Confidence interval, MESH: Candidiasis, Invasive, MESH: Randomized Controlled Trials as Topic, Immunology, Diagnostic odds ratio, MESH: Europe, business, MESH: Congresses as Topic
Abstract: International audience; INTRODUCTION: Timely diagnosis of invasive candidiasis (IC) remains difficult as the clinical presentation is not specific and blood cultures lack sensitivity and need a long incubation time. Thus, non-culture-based methods for diagnosing IC have been developed. Mannan antigen (Mn) and anti-mannan antibodies (A-Mn) are present in patients with IC. On behalf of the Third European Conference on Infections in Leukemia, the performance of these tests was analysed and reviewed. METHODS: The literature was searched for studies using the commercially available sandwich enzyme-linked immunosorbent assays (Platelia™, Bio-Rad Laboratories, Marnes-la-Coquette, France) for detecting Mn and A-Mn in serum. The target condition of this review was IC defined according to 2008 European Organization for Research and Treatment of Cancer/Mycoses Study Group criteria. Sensitivity, specificity and diagnostic odds ratios (DOR) were calculated for Mn, A-Mn and combined Mn/A-Mn testing. RESULTS: Overall, 14 studies that comprised 453 patients and 767 controls were reviewed. The patient populations included in the studies were mainly haematological and cancer cases in seven studies and mainly intensive care unit and surgery cases in the other seven studies. All studies but one were retrospective in design. Mn sensitivity was 58% (95% confidence interval [CI], 53-62); specificity, 93% (95% CI, 91-94) and DOR, 18 (95% CI 12-28). A-Mn sensitivity was 59% (95% CI, 54-65); specificity, 83% (95% CI, 79-97) and DOR, 12 (95% CI 7-21). Combined Mn/A-Mn sensitivity was 83% (95% CI, 79-87); specificity, 86% (95% CI, 82-90) and DOR, 58 (95% CI 27-122). Significant heterogeneity of the studies was detected. The sensitivity of both Mn and A-Mn varied for different Candida species, and it was the highest for C. albicans, followed by C. glabrata and C. tropicalis. In 73% of 45 patients with candidemia, at least one of the serological tests was positive before the culture results, with mean time advantage being 6 days for Mn and 7 days for A-Mn. In 21 patients with hepatosplenic IC, 18 (86%) had Mn or A-Mn positive test results at a median of 16 days before radiological detection of liver or spleen lesions. CONCLUSIONS: Mn and A-Mn are useful for diagnosis of IC. The performance of combined Mn/A-Mn testing is superior to either Mn or A-Mn testing.
Published: 2010

12. Surface hydrophobin prevents immune recognition of airborne fungal spores

Author: Axel A. Brakhage, Jagadeesh Bayry, Jean-Paul Latgé, Cécile Clavaud, Sriramulu Elluru, Srini V. Kaveri, Sophie Paris, Silvia Bozza, Luigina Romani, Olaf Kniemeyer, Katia Perruccio, Vishukumar Aimanianda, Aspergillus, Institut Pasteur [Paris], Centre de Recherche des Cordeliers (CRC), Université Paris Diderot - Paris 7 (UPD7)-École pratique des hautes études (EPHE)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Pierre et Marie Curie - Paris 6 - UFR de Médecine Pierre et Marie Curie (UPMC), Université Pierre et Marie Curie - Paris 6 (UPMC), Université Sorbonne Paris Cité (USPC), Institut National de la Santé et de la Recherche Médicale (INSERM), Università degli Studi di Perugia (UNIPG), Leibniz Institute for Natural Product Research and Infection Biology (Hans Knoell Institute), Department of Clinical and Experimental Medicine, Linköping University (LIU), Université Grenoble Alpes (UGA), Friedrich-Schiller-Universität Jena, Centre de Recherche des Cordeliers (CRC (UMR_S 872)), Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche des Cordeliers ( CRC ), Université Paris Diderot - Paris 7 ( UPD7 ) -École pratique des hautes études ( EPHE ) -Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), University of Perugia - Italy, University of Perugia, Université Paris Diderot - Paris 7 (UPD7)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Aimanianda, Vishukumar, Institut Pasteur [Paris] (IP), Université Pierre et Marie Curie - Paris 6 (UPMC)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Università degli Studi di Perugia = University of Perugia (UNIPG), and Université Pierre et Marie Curie - Paris 6 (UPMC)-École Pratique des Hautes Études (EPHE)
Subjects: CD4-Positive T-Lymphocytes, MESH : Spores, Fungal, MESH: Macrophages, Alveolar, MESH : Hydrofluoric Acid, [SDV]Life Sciences [q-bio], Galactosaminogalactan, Air Microbiology, Lymphocyte Activation, [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity, Aspergillus fumigatus, chemistry.chemical_compound, MESH : Antigens, Fungal, Mice, MESH: Air Microbiology, MESH : Fungal Proteins, MESH: Animals, [SDV.IMM.ALL]Life Sciences [q-bio]/Immunology/Allergology, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, MESH : Allergens, [ SDV.MP.MYC ] Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, Cells, Cultured, ComputingMilieux_MISCELLANEOUS, 0303 health sciences, Fungal protein, Multidisciplinary, biology, MESH: Dendritic Cells, MESH: Antigens, Plant, MESH : Adoptive Transfer, MESH: CD4-Positive T-Lymphocytes, Spores, Fungal, Adoptive Transfer, [SDV] Life Sciences [q-bio], MESH : Macrophages, Alveolar, MESH : CD4-Positive T-Lymphocytes, MESH: Fungal Proteins, MESH: Aspergillus fumigatus, Cladosporium, MESH: Cells, Cultured, MESH: Allergens, Antigens, Fungal, Hydrophobin, MESH : Air Microbiology, MESH : Mice, Inbred C57BL, MESH : Cathepsins, Hydrofluoric Acid, Microbiology, MESH: Hydrofluoric Acid, Fungal Proteins, 03 medical and health sciences, Immune system, MESH: Mice, Inbred C57BL, MESH: Spores, Fungal, MESH : Mice, MESH : Cells, Cultured, Macrophages, Alveolar, Animals, Humans, MESH: Lymphocyte Activation, [SDV.IMM.II] Life Sciences [q-bio]/Immunology/Innate immunity, MESH: Mice, MESH : Lymphocyte Activation, 030304 developmental biology, MESH: Antigens, Fungal, [ SDV.IMM.II ] Life Sciences [q-bio]/Immunology/Innate immunity, Innate immune system, MESH: Humans, MESH: Immune System, [ SDV ] Life Sciences [q-bio], 030306 microbiology, fungi, MESH : Humans, Dendritic cell, Dendritic Cells, Allergens, Antigens, Plant, biology.organism_classification, [SDV.MP.MYC] Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, Cathepsins, Mice, Inbred C57BL, MESH: Adoptive Transfer, MESH : Antigens, Plant, chemistry, MESH : Dendritic Cells, MESH : Aspergillus fumigatus, Immune System, MESH: Cathepsins, MESH : Immune System, MESH : Animals
Abstract: International audience; The air we breathe is filled with thousands of fungal spores (conidia) per cubic metre, which in certain composting environments can easily exceed 10(9) per cubic metre. They originate from more than a hundred fungal species belonging mainly to the genera Cladosporium, Penicillium, Alternaria and Aspergillus. Although these conidia contain many antigens and allergens, it is not known why airborne fungal microflora do not activate the host innate immune cells continuously and do not induce detrimental inflammatory responses following their inhalation. Here we show that the surface layer on the dormant conidia masks their recognition by the immune system and hence prevents immune response. To explore this, we used several fungal members of the airborne microflora, including the human opportunistic fungal pathogen Aspergillus fumigatus, in in vitro assays with dendritic cells and alveolar macrophages and in in vivo murine experiments. In A. fumigatus, this surface 'rodlet layer' is composed of hydrophobic RodA protein covalently bound to the conidial cell wall through glycosylphosphatidylinositol-remnants. RodA extracted from conidia of A. fumigatus was immunologically inert and did not induce dendritic cell or alveolar macrophage maturation and activation, and failed to activate helper T-cell immune responses in vivo. The removal of this surface 'rodlet/hydrophobin layer' either chemically (using hydrofluoric acid), genetically (DeltarodA mutant) or biologically (germination) resulted in conidial morphotypes inducing immune activation. All these observations show that the hydrophobic rodlet layer on the conidial cell surface immunologically silences airborne moulds.
Published: 2009

13. Diagnostic value of serum precipitins to mould antigens in active hypersensitivity pneumonitis

Author: Sandrine Roussel, Gabriel Reboux, C.-M. Fenoglio, Bertrand Sudre, Mariette Mercier, J.F. Cordier, Renaud Piarroux, Jean-Charles Dalphin, Carcinogénèse épithéliale : facteurs prédictifs et pronostiques - UFC ( CEF2P / CARCINO ), Université Bourgogne Franche-Comté ( UBFC ) -Université de Franche-Comté ( UFC ) -Centre Hospitalier Régional Universitaire [Besançon] ( CHRU Besançon ), Laboratoire Chrono-environnement ( LCE ), Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Carcinogénèse épithéliale : facteurs prédictifs et pronostiques - UFC (EA 3181) (CEF2P / CARCINO), Université de Franche-Comté (UFC), and Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)
Subjects: Male, MESH : Aged, Serology, [ SDV.CAN ] Life Sciences [q-bio]/Cancer, Diffusion, MESH : Antigens, Fungal, 0302 clinical medicine, Saccharopolyspora rectivirgula, MESH : Female, 030212 general & internal medicine, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, [ SDV.MP.MYC ] Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, MESH: Aged, PROSPECTIVE STUDY, MESH: Middle Aged, medicine.diagnostic_test, biology, MESH : Models, Statistical, MESH: Diffusion, Middle Aged, MESH : Adult, Precipitin, MESH: Predictive Value of Tests, 3. Good health, Predictive value of tests, Female, MESH : Sensitivity and Specificity, Counterimmunoelectrophoresis, HYPERSENSITIVITY PNEUMONITIS, Hypersensitivity pneumonitis, Alveolitis, Extrinsic Allergic, Adult, Pulmonary and Respiratory Medicine, MESH: Fungi, Antigens, Fungal, MESH: Counterimmunoelectrophoresis, MESH : Precipitins, MESH : Male, MESH : Alveolitis, Extrinsic Allergic, MESH : Counterimmunoelectrophoresis, [SDV.CAN]Life Sciences [q-bio]/Cancer, MESH: Alveolitis, Extrinsic Allergic, MESH: Precipitins, DIAGNOSIS, Sensitivity and Specificity, 03 medical and health sciences, Predictive Value of Tests, medicine, Humans, PRECIPITINS, MESH : Middle Aged, MESH : Predictive Value of Tests, MESH : Diffusion, MESH : Fungi, Aged, MESH: Antigens, Fungal, Models, Statistical, MESH: Humans, business.industry, SEROLOGY, MESH : Humans, Fungi, MESH: Adult, biology.organism_classification, medicine.disease, MESH: Sensitivity and Specificity, MESH: Male, Bronchoalveolar lavage, 030228 respiratory system, Immunology, Differential diagnosis, business, MESH: Female, MESH: Models, Statistical
Abstract: International audience; Serum precipitins have a controversial diagnostic value in hypersensitivity pneumonitis (HP). The present authors' objective was to assess their diagnostic value by developing scores from a panel of specific antigens tested by two techniques (electrosyneresis and double diffusion) to discriminate active HP from other interstitial lung diseases. Consecutive patients presenting with a condition for which HP was considered in the differential diagnosis were included in the study. All patients underwent the same standardised diagnostic procedure, including precipitin tests performed in routine conditions. Clinical manifestations, bronchoalveolar lavage and high-resolution computed tomography defined the presence or absence of HP. Receiver-operating characteristic curves and logistic regression were used to develop the serological scores. A total of 122 patients (including 31 cases of HP) were included in the study. Five antigens from the panel were selected for the serological scores (Absidia corymbifera, Eurotium amstelodami, Wallemia sebi, Saccharopolyspora rectivirgula and mesophilic Streptomyces sp.). Electrosyneresis was more discriminative than the double-diffusion technique. Predictive negative values varied 81-88% and predictive positive values varied 71-75% for prevalence of HP 20-35%. In conclusion, serological scores using a panel of relevant antigens may guide both biological and clinical practice in areas of high prevalence of hypersensitivity pneumonitis.
Published: 2007

14. Comparison of epidemiological, clinical, and biological features of invasive aspergillosis in neutropenic and nonneutropenic patients: a 6-year survey

Author: Jean-Pierre Gangneux, Sylvie Caulet-Maugendre, Emmanuelle Leray, M. Faucheux, Virginie Gandemer, C. Meunier, Bernard Lelong, A. Cornillet, C. Lebert, Chantal Belleguic, Christophe Camus, Claude Guiguen, Stanislas Nimubona, Pierre Tattevin, Sylviane Chevrier, M. Aupee, CHU Pontchaillou [Rennes], Service des urgences [Rennes] = Emergency [Rennes], Service des maladies infectieuses et réanimation médicale [Rennes] = Infectious Disease and Intensive Care [Rennes], Service d'hématologie-oncologie pédiatrique, Service de pneumologie, Service de Parasitologie-Mycologie [Rennes], Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Hôpital Pontchaillou-CHU Pontchaillou [Rennes], Génétique et évolution des maladies infectieuses (GEMI), Centre National de la Recherche Scientifique (CNRS)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [France-Sud]), Signalisation et Réponses aux Agents Infectieux et Chimiques (SeRAIC), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Service d'hématologie clinique, Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Hôpital Pontchaillou, Centre de recherches (CRT), Société Lafarge, Service de cardiologie et maladies vasculaires [Rennes] = Cardiac, Thoracic, and Vascular Surgery [Rennes], Comportement et noyaux gris centraux = Behavior and Basal Ganglia [Rennes], Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Université européenne de Bretagne - European University of Brittany (UEB)-CHU Pontchaillou [Rennes]-Institut des Neurosciences Cliniques de Rennes (INCR), Service de santé publique et d'épidémiologie, Hôpital Pontchaillou-CHU Pontchaillou [Rennes], Université de Rennes (UR)-Hôpital Pontchaillou-CHU Pontchaillou [Rennes], Université Montpellier 1 (UM1)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Université de Rennes (UR), Université de Rennes (UR)-Hôpital Pontchaillou, and Université de Rennes (UR)-Université européenne de Bretagne - European University of Brittany (UEB)-CHU Pontchaillou [Rennes]-Institut des Neurosciences Cliniques de Rennes = Institute of Clinical Neurosciences of Rennes (INCR)
Subjects: Male, Antifungal Agents, Time Factors, Opportunistic infection, Aspergillosis, law.invention, 0302 clinical medicine, law, Risk Factors, MESH: Risk Factors, MESH: Child, 030212 general & internal medicine, Child, MESH: Aged, 0303 health sciences, Leukopenia, MESH: Middle Aged, Mortality rate, Middle Aged, Intensive care unit, 3. Good health, Infectious Diseases, Fungal, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Child, Preschool, Female, medicine.symptom, Microbiology (medical), Adult, medicine.medical_specialty, Antigens, Fungal, Neutropenia, Adolescent, MESH: Neutropenia, Antibodies, 03 medical and health sciences, Internal medicine, medicine, Humans, MESH: Aspergillosis, Antigens, Preschool, Antibodies, Fungal, Mycosis, Aged, MESH: Antigens, Fungal, MESH: Adolescent, MESH: Humans, 030306 microbiology, business.industry, MESH: Time Factors, MESH: Antibodies, Fungal, MESH: Child, Preschool, MESH: Adult, medicine.disease, MESH: Antifungal Agents, MESH: Male, Surgery, Pneumonia, business, MESH: Female
Abstract: International audience; BACKGROUND: Invasive aspergillosis is an opportunistic infection that occurs mainly among patients with prolonged neutropenia. Few data are available on invasive aspergillosis in nonneutropenic patients. METHODS: The aim of this survey was to compare neutropenic and nonneutropenic patients who had received a diagnosis of invasive aspergillosis at our institution during a 6-year period. RESULTS: Among the 88 cases of invasive aspergillosis analyzed here, 12 were histologically proven, 52 were probable, and 24 were possible. Forty-seven percent of cases were diagnosed in the intensive care unit, and 40% were diagnosed in hematology units. Neutropenia was a risk factor for 52 patients (59%), most of whom had hematological or solid malignancies. Among the 36 nonneutropenic patients (41%), the main underlying conditions were steroid-treated chronic obstructive pulmonary disease, asthma, rheumatoid arthritis, giant-cell arteritis, and microvascular disorders; 10 patients were recipients of solid-organ transplants, and 1 patient was seropositive for human immunodeficiency virus. The distribution of proven and probable invasive aspergillosis was similar for neutropenic and nonneutropenic patients. The mortality rate was 71.5% overall and was significantly higher among nonneutropenic patients than among neutropenic patients (89% vs. 60%; P
Published: 2006

15. Pulmonary cryptococcosis: localized and disseminated infections in 27 patients with AIDS

Author: J. L. Meynard, Christos Chouaid, Patricia Roux, Willy Rozenbaum, J. Frottier, Poirot Jl, Marie-Caroline Meyohas, Diane Bollens, Charles Mayaud, Laboratoire de Géophysique Interne et Tectonophysique (LGIT), Laboratoire Central des Ponts et Chaussées (LCPC)-Observatoire des Sciences de l'Univers de Grenoble (OSUG), Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national des sciences de l'Univers (INSU - CNRS)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national des sciences de l'Univers (INSU - CNRS)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Epidémiologie des maladies infectieuses et modélisation (ESIM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Tenon [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Meyohas MC, P Roux, Bollens D, C Chouaid, W Rozenbaum, JL Meynard, JL Poirot, Frottier J, C Mayaud, Observatoire des Sciences de l'Univers de Grenoble (OSUG), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Laboratoire Central des Ponts et Chaussées (LCPC)-Institut des Sciences de la Terre (ISTerre), Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-PRES Université de Grenoble-Institut de recherche pour le développement [IRD] : UR219-Institut national des sciences de l'Univers (INSU - CNRS)-Institut Français des Sciences et Technologies des Transports, de l'Aménagement et des Réseaux (IFSTTAR)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-PRES Université de Grenoble-Institut de recherche pour le développement [IRD] : UR219-Institut Français des Sciences et Technologies des Transports, de l'Aménagement et des Réseaux (IFSTTAR), CHU Tenon [APHP], Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national des sciences de l'Univers (INSU - CNRS)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national des sciences de l'Univers (INSU - CNRS)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Laboratoire Central des Ponts et Chaussées (LCPC)-Centre National de la Recherche Scientifique (CNRS), and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)
Subjects: Male, Pathology, MESH: CD4 Lymphocyte Count, Pleural effusion, MESH: Lung Diseases, Fungal, 0302 clinical medicine, 030212 general & internal medicine, MESH: Middle Aged, medicine.diagnostic_test, biology, MESH: AIDS-Related Opportunistic Infections, Respiratory disease, MESH: Cryptococcus neoformans, Cryptococcosis, Middle Aged, Prognosis, 3. Good health, Infectious Diseases, Female, Bronchoalveolar Lavage Fluid, Microbiology (medical), Adult, medicine.medical_specialty, Antigens, Fungal, AIDS-Related Opportunistic Infections, MESH: Cryptococcosis, MESH: Prognosis, 03 medical and health sciences, medicine, Humans, Cryptococcal Pneumonia, Mycosis, MESH: Antigens, Fungal, Cryptococcus neoformans, MESH: Humans, Lung Diseases, Fungal, business.industry, MESH: Bronchoalveolar Lavage Fluid, MESH: Adult, biology.organism_classification, medicine.disease, MESH: Male, CD4 Lymphocyte Count, Bronchoalveolar lavage, 030228 respiratory system, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, business, MESH: Female
Abstract: International audience; We reviewed the records of 85 patients infected with both human immunodeficiency virus and Cryptococcus neoformans. Twenty-seven patients (32%) had pulmonary cryptococcosis. C. neoformans was cultured from bronchoalveolar lavage (BAL) or pleural fluid in 25 cases; the remaining two patients had cryptococcal antigen (CA) detected in BAL fluid and C. neoformans cultured from other sites. All but one of the 27 patients had detectable CA in serum. The CD4+ lymphocyte count was low in all cases (median, 24/mm3). Clinical manifestations of pulmonary cryptococcosis included fever (94%), cough (71%), dyspnea (7%), expectoration (4%), chest pain (2%), and hemoptysis (1%). Diffuse interstitial opacities (70.5%), focal interstitial abnormalities, alveolar opacities, adenopathies, cavitary lesions, and pleural effusions were evident. Outcome was poor (mean survival time, 23 weeks) despite treatment. Patients with localized pulmonary cryptococcosis appeared to have a higher CD4+ lymphocyte count, an earlier diagnosis, lower serum CA titers, fewer previous or concomitant infections, and a better prognosis than patients with disseminated cryptococcosis.
Published: 1995

16. Usefulness of PCR for detection of Pneumocystis carinii DNA

Author: Christos Chouaid, Patricia Roux, M Nigou, Michel Denis, J L Olivier, I Lavrard, Poirot Jl, M Miltgen, Laboratoire de Géophysique Interne et Tectonophysique (LGIT), Observatoire des Sciences de l'Univers de Grenoble (OSUG), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Laboratoire Central des Ponts et Chaussées (LCPC)-Institut des Sciences de la Terre (ISTerre), Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-PRES Université de Grenoble-Institut de recherche pour le développement [IRD] : UR219-Institut national des sciences de l'Univers (INSU - CNRS)-Institut Français des Sciences et Technologies des Transports, de l'Aménagement et des Réseaux (IFSTTAR)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-PRES Université de Grenoble-Institut de recherche pour le développement [IRD] : UR219-Institut Français des Sciences et Technologies des Transports, de l'Aménagement et des Réseaux (IFSTTAR), Epidémiologie des maladies infectieuses et modélisation (ESIM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de MicrobiologiE de Géochimie et d'Ecologie Marines (LMGEM), Université de la Méditerranée - Aix-Marseille 2-Centre National de la Recherche Scientifique (CNRS), P Roux, je Lavrard, JL Poirot, C Chouaid, M Denis, Olivier JL, M Nigou, M Miltgen, Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national des sciences de l'Univers (INSU - CNRS)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national des sciences de l'Univers (INSU - CNRS)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Laboratoire Central des Ponts et Chaussées (LCPC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Central des Ponts et Chaussées (LCPC)-Observatoire des Sciences de l'Univers de Grenoble (OSUG), Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national des sciences de l'Univers (INSU - CNRS)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national des sciences de l'Univers (INSU - CNRS)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), and Centre National de la Recherche Scientifique (CNRS)-Université de la Méditerranée - Aix-Marseille 2
Subjects: Microbiology (medical), Antigens, Fungal, AIDS-Related Opportunistic Infections, Fluorescent Antibody Technique, Biology, Polymerase Chain Reaction, Sensitivity and Specificity, Microbiology, law.invention, 03 medical and health sciences, 0302 clinical medicine, law, MESH: Fungemia, medicine, Humans, 030212 general & internal medicine, DNA, Fungal, MESH: Pneumocystis, MESH: Fluorescent Antibody Technique, Polymerase chain reaction, MESH: Antigens, Fungal, 0303 health sciences, MESH: Humans, medicine.diagnostic_test, 030306 microbiology, Pneumocystis, MESH: AIDS-Related Opportunistic Infections, MESH: Bronchoalveolar Lavage Fluid, Pneumonia, Pneumocystis, Respiratory disease, MESH: Polymerase Chain Reaction, medicine.disease, MESH: Sensitivity and Specificity, 3. Good health, Staining, respiratory tract diseases, MESH: DNA, Fungal, Pneumonia, Bronchoalveolar lavage, Pneumocystis carinii, Sputum, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, medicine.symptom, Bronchoalveolar Lavage Fluid, Fungemia, Research Article, MESH: Pneumonia, Pneumocystis
Abstract: International audience; Diagnosis of Pneumocystis carinii pneumonia is based on the identification of the various stages of the parasite in lung samples by standard staining techniques. We therefore assessed the value of the PCR for detection of P. carinii in bronchoalveolar lavage, induced sputum, and blood samples relative to that of standard staining techniques.
Published: 1994

17. Galactosaminogalactan, a New Immunosuppressive Polysaccharide of Aspergillus fumigatus

Author: Silvia Moretti, Markus Aebi, Jean-Paul Latgé, Muriel Delepierre, Flavio Schwarz, Aurélie Delangle, Sandra J. van Vliet, Catherine Simenel, Yvette van Kooyk, Bernadette Coddeville, Coline Trichot, Luigina Romani, Silvia Bozza, Carole Elbim, Thierry Fontaine, Aspergillus, Institut Pasteur [Paris], Résonance Magnétique Nucléaire des Biomolécules, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), VU University Medical Center [Amsterdam], Università degli Studi di Perugia (UNIPG), Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich), Immunité et Infection, Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR113-Sorbonne Université (SU), This work was partly supported by a grant from Agence Nationale de la Recherche (ANR-06-EMPB-011-01) and by European grants Allfun, Fungwall, ANRpathogenomics. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript., ANR-06-EMPB-0011,GALNACGAL,Développement d'un nouveau test de diagnostic de l'aspergillose(2006), European Project: 260338,EC:FP7:HEALTH,FP7-HEALTH-2010-single-stage,ALLFUN(2010), European Project: 511952,FUNGWALL(2005), European Project: 27618,PATHOGENOMICS, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS ), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 ( UGSF ), Institut National de la Recherche Agronomique ( INRA ) -Université de Lille-Centre National de la Recherche Scientifique ( CNRS ), University of Amsterdam [Amsterdam] ( UvA ), Università degli Studi di Perugia ( UNIPG ), Eidgenössische Technische Hochschule [Zürich] ( ETH Zürich ), Université Pierre et Marie Curie - Paris 6 ( UPMC ) -IFR113-Institut National de la Santé et de la Recherche Médicale ( INSERM ), This work was partly supported by a grant from Agence Nationale de la Recherche (ANR-06-EMPB-011-01) and by European grants Allfun, Fungwall, ANRpathogenomics., ANR-06-EMPB-0011,GALNACGAL,Développement d'un nouveau test de diagnostic de l'aspergillose ( 2006 ), Institut Pasteur [Paris] (IP), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Università degli Studi di Perugia = University of Perugia (UNIPG), IFR113-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology in Zürich [Zürich] (ETH Zürich), CCA - Immuno-pathogenesis, Molecular cell biology and Immunology, Andriakoto, Nirina, Emergence et maturation de projets de biotechnologie à fort potentiel de valorisation (EMPB) - Développement d'un nouveau test de diagnostic de l'aspergillose - - GALNACGAL2006 - ANR-06-EMPB-0011 - EMPB - VALID, Fungi in the setting of inflammation, allergy and autoimmune diseases:Translating basic science into clinical practices - ALLFUN - - EC:FP7:HEALTH2010-12-01 - 2014-05-31 - 260338 - VALID, The Fungal cell wall as a target for antifungal therapies - FUNGWALL - 2005-01-01 - 2007-12-31 - 511952 - OLD, and Trans-European cooperation and coordination of genome sequencing and functional genomics of human-pathogenic microorganisms - PATHOGENOMICS - 27618 - OLD
Subjects: Magnetic Resonance Spectroscopy, MESH : Polysaccharides, Galactosaminogalactan, Apoptosis, MESH: Neutrophils, Biochemistry, Epitope, Aspergillus fumigatus, MESH : Neutrophils, Epitopes, Mice, MESH : Antigens, Fungal, Cell Wall, MESH : Antibodies, Fungal, MESH : Aspergillosis, MESH: Animals, Fungal Biochemistry, MESH : Immunosuppressive Agents, Immune Response, lcsh:QH301-705.5, [ SDV.MP.MYC ] Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, MESH : Macrophages, chemistry.chemical_classification, 0303 health sciences, education.field_of_study, MESH : Cross Reactions, 3. Good health, Neutrophil Infiltration, Carbohydrate conformation, MESH: Immunosuppressive Agents, Antigens, Fungal, MESH: Epitopes, Immunology, Cross Reactions, MESH : Epitopes, Microbiology, 03 medical and health sciences, MESH: Cell Wall, Genetics, Aspergillosis, Humans, education, Biology, Molecular Biology, Antibodies, Fungal, MESH: Antigens, Fungal, Aspergillus, MESH: Humans, [ SDV ] Life Sciences [q-bio], 030306 microbiology, MESH: Magnetic Resonance Spectroscopy, Macrophages, MESH : Humans, MESH: Host-Pathogen Interactions, [SDV.MP.MYC] Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, Mice, Inbred C57BL, MESH : Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, chemistry, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, MESH : Magnetic Resonance Spectroscopy, Parasitology, lcsh:RC581-607, MESH: Female, MESH : Apoptosis, Neutrophils, Glycobiology, MESH: Cross Reactions, chemistry.chemical_compound, Carbohydrate Conformation, MESH : Female, MESH: Carbohydrate Conformation, MESH : Host-Pathogen Interactions, biology, Host-Pathogen Interaction, MESH : Neutrophil Infiltration, Carbohydrate Sequence, Host-Pathogen Interactions, MESH : Carbohydrate Sequence, Female, MESH: Aspergillus fumigatus, Immunosuppressive Agents, Research Article, lcsh:Immunologic diseases. Allergy, Population, Immunopathology, Mycology, MESH : Mice, Inbred C57BL, Polysaccharide, Campylobacter jejuni, Polysaccharides, MESH: Mice, Inbred C57BL, Virology, MESH : Mice, Animals, MESH: Aspergillosis, MESH: Mice, 030304 developmental biology, MESH : Cell Wall, MESH: Carbohydrate Sequence, MESH: Apoptosis, MESH: Antibodies, Fungal, Fungi, MESH: Macrophages, biology.organism_classification, MESH: Neutrophil Infiltration, MESH: Polysaccharides, MESH: Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, lcsh:Biology (General), MESH : Aspergillus fumigatus, MESH : Carbohydrate Conformation, MESH : Animals
Abstract: A new polysaccharide secreted by the human opportunistic fungal pathogen Aspergillus fumigatus has been characterized. Carbohydrate analysis using specific chemical degradations, mass spectrometry, 1H and 13C nuclear magnetic resonance showed that this polysaccharide is a linear heterogeneous galactosaminogalactan composed of α1-4 linked galactose and α1-4 linked N-acetylgalactosamine residues where both monosacharides are randomly distributed and where the percentage of galactose per chain varied from 15 to 60%. This polysaccharide is antigenic and is recognized by a majority of the human population irrespectively of the occurrence of an Aspergillus infection. GalNAc oligosaccharides are an essential epitope of the galactosaminogalactan that explains the universal antibody reaction due to cross reactivity with other antigenic molecules containing GalNAc stretches such as the N-glycans of Campylobacter jejuni. The galactosaminogalactan has no protective effect during Aspergillus infections. Most importantly, the polysaccharide promotes fungal development in immunocompetent mice due to its immunosuppressive activity associated with disminished neutrophil infiltrates., Author Summary Aspergillus fumigatus is an opportunistic human fungal pathogen that causes a wide range of diseases including allergic reactions and local or systemic infections such as invasive pulmonary aspergillosis that has emerged in the recent years as a leading cause of infection related mortality among immunocompromised patients. Polysaccharides from the fungal cell wall play essential biological functions in the fungal cell biology and in host-pathogen interactions. Indeed, it has been shown that polysaccharides can modulate the human immune response; some of them (β-glucan and α-glucans) having a protective effect against Aspergillus infection. We report here the purification and chemical characterization of a new antigenic polysaccharide (galactosaminogalactan) produced by A. fumigatus. This polymer is secreted during infection. In murine models of aspergillosis, this galactosaminogalactan is not protective but it is immunosuppressive and favors A. fumigatus infection. Particularly it induces the apoptotic death of neutrophils that are the phagocytes playing an essential role in the killing of fungal pathogens.
Published: 2011
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17 results on '"MESH: Antigens, Fungal"'

1. Novel mouse monoclonal antibodies specifically recognizing β-(1→3)-D-glucan antigen

2. Exposure to field vs. storage wheat dust: different consequences on respiratory symptoms and immune response among grain workers

3. Fungal peptides from pneumonitis hypersensitivity etiologic agents are able to induce specific cellular immune response

4. Surveillance and management of Echinococcus multilocularis in a wildlife park

5. Respiratory health effects of fifteen years of improved collective protection in a wheat-processing worker population

6. Serum Aspergillus Galactomannan for the Management of Disseminated Histoplasmosis in AIDS

7. Diagnosis, management and outcome of Candida endocarditis

8. Prospective evaluation of clinical and biological markers to predict the outcome of invasive pulmonary aspergillosis in hematological patients

9. Anti-inflammatory effect of fluvastatin on IL-8 production induced by Pseudomonas aeruginosa and Aspergillus fumigatus antigens in cystic fibrosis

10. Effects of age, gender and time on receptor expression and anti-Aspergillus functions of human phagocytes

11. The use of mannan antigen and anti-mannan antibodies in the diagnosis of invasive candidiasis: recommendations from the Third European Conference on Infections in Leukemia

12. Surface hydrophobin prevents immune recognition of airborne fungal spores

13. Diagnostic value of serum precipitins to mould antigens in active hypersensitivity pneumonitis

14. Comparison of epidemiological, clinical, and biological features of invasive aspergillosis in neutropenic and nonneutropenic patients: a 6-year survey

15. Pulmonary cryptococcosis: localized and disseminated infections in 27 patients with AIDS

16. Usefulness of PCR for detection of Pneumocystis carinii DNA

17. Galactosaminogalactan, a New Immunosuppressive Polysaccharide of Aspergillus fumigatus

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17 results on '"MESH: Antigens, Fungal"'

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