18 results on '"MDRE"'
Search Results
2. DYSF promotes monocyte activation in atherosclerotic cardiovascular disease as a DNA methylation-driven gene.
- Author
-
Zhang, Xiaokang, He, Dingdong, Xiang, Yang, Wang, Chen, Liang, Bin, Li, Boyu, Qi, Daoxi, Deng, Qianyun, Yu, Hong, Lu, Zhibing, and Zheng, Fang
- Abstract
Dysferlin (DYSF) has drawn much attention due to its involvement in dysferlinopathy and was reported to affect monocyte functions in recent studies. However, the role of DYSF in the pathogenesis of atherosclerotic cardiovascular diseases (ASCVD) and the regulation mechanism of DYSF expression have not been fully studied. In this study, Gene Expression Omnibus (GEO) database and epigenome-wide association study (EWAS) literatures were searched to find the DNA methylation-driven genes (including DYSF) of ASCVD. The hub genes related to DYSF were also identified through weighted correlation network analysis (WGCNA). Regulation of DYSF expression through its promoter methylation status was verified using peripheral blood leucocytes (PBLs) from ASCVD patients and normal controls, and experiments on THP1 cells and Apoe-/- mice. Similarly, the expressions of DYSF related hub genes, mainly contained SELL, STAT3 and TMX1, were also validated. DYSF functions were then evaluated by phagocytosis, transwell and adhesion assays in DYSF knock-down and overexpressed THP1 cells. The results showed that DYSF promoter hypermethylation up-regulated its expression in clinical samples, THP1 cells and Apoe-/- mice, confirming DYSF as a DNA methylation-driven gene. The combination of DYSF expression and methylation status in PBLs had a considerable prediction value for ASCVD. Besides, DYSF could enhance the phagocytosis, migration and adhesion ability of THP1 cells. Among DYSF related hub genes, SELL was proven to be the downstream target of DYSF by wet experiments. In conclusion, DYSF promoter hypermethylation upregulated its expression and promoted monocytes activation, which further participated in the pathogenesis of ASCVD. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
3. Trends in Occurrence and Phenotypic Resistance of Coagulase-Negative Staphylococci (CoNS) Found in Human Blood in the Northern Netherlands between 2013 and 2019.
- Author
-
Berends, Matthijs S., Luz, Christian F., Ott, Alewijn, Andriesse, Gunnar I., Becker, Karsten, Glasner, Corinna, and Friedrich, Alex W.
- Abstract
Background: For years, coagulase-negative staphylococci (CoNS) were not considered a cause of bloodstream infections (BSIs) and were often regarded as contamination. However, the association of CoNS with nosocomial infections is increasingly recognized. The identification of more than 40 different CoNS species has been driven by the introduction of matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Yet, treatment guidelines consider CoNS as a whole group, despite increasing antibiotic resistance (ABR) in CoNS. This retrospective study provides an in-depth data analysis of CoNS isolates found in human blood culture isolates between 2013 and 2019 in the entire region of the Northern Netherlands. Methods: In total, 10,796 patients were included that were hospitalized in one of the 15 hospitals in the region, leading to 14,992 CoNS isolates for (ABR) data analysis. CoNS accounted for 27.6% of all available 71,632 blood culture isolates. EUCAST Expert rules were applied to correct for errors in antibiotic test results. Results: A total of 27 different CoNS species were found. Major differences were observed in occurrence and ABR profiles. The top five species covered 97.1% of all included isolates: S. epidermidis, S. hominis, S. capitis, S. haemolyticus, and S. warneri. Regarding ABR, methicillin resistance was most frequently detected in S. haemolyticus (72%), S. cohnii (65%), and S. epidermidis (62%). S. epidermidis and S. haemolyticus showed 50–80% resistance to teicoplanin and macrolides while resistance to these agents remained lower than 10% in most other CoNS species. Conclusion: These differences are often neglected in national guideline development, prompting a focus on 'ABR-safe' agents such as glycopeptides. In conclusion, this multi-year, full-region approach to extensively assess the trends in both the occurrence and phenotypic resistance of CoNS species could be used for evaluating treatment policies and understanding more about these important but still too often neglected pathogens. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
4. Clinical Feature, Therapy, Antimicrobial Resistance Gene Distribution, and Outcome of Nosocomial Meningitis Induced by Multidrug-Resistant Enterobacteriaceae—A Longitudinal Cohort Study From Two Neurosurgical Centers in Northern China.
- Author
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Zheng, Guanghui, Shi, Yijun, Cao, Yanfei, Qian, Lingye, Lv, Hong, Zhang, Lina, and Zhang, Guojun
- Subjects
MULTIDRUG-resistant tuberculosis ,MENINGITIS ,DRUG resistance in microorganisms ,MORTALITY risk factors ,MEDICAL record databases ,MULTIDRUG resistance ,PROPORTIONAL hazards models - Abstract
Objectives: This is a comparative cohort study aiming to evaluate the mortality risk factors for patients with nosocomial meningitis (NM) induced by multidrug-resistant Enterobacteriaceae (MDRE) in China. The clinical features and therapies of patients and the resistance mechanisms of MDRE pathogens were also assessed. Methods: MDRE-NM patients from two neurosurgical centers in China from 2014 to 2019 were included in this study. Clinical features were extracted from the medical record databases of the two centers. The molecular mechanisms underlying the microbiological resistance mechanisms of each MDRE pathogen were determined, Kaplan–Meier survival analysis was conducted, and multivariable analyses were performed using a Cox proportional hazard model. Results: Ninety MDRE-NM patients were included in this study. Klebsiella pneumoniae accounted for the highest proportion of causative pathogens (46/90, 51.1%), and 40 causative pathogens (44.4%) were meropenem-resistant. blaKPC (27/40, 67.5%) was the predominant carbapenem resistance gene. Multivariate Cox analysis showed that external ventricular drainage (EVD) [hazard ratio (HR) = 2.524, 95% confidence interval (CI) = 1.101–5.787, p = 0.029] and a Glasgow Coma Scale (GCS) score ≤;8 (HR = 4.033, 95% CI = 1.526–10.645, p = 0.005) were mortality risk factors for patients with MDRE-NM. A total of 90.0%, 94.4%, and 97.8% of MDRE-NM patients received antibiotic prophylaxis (AP), antibiotic empirical therapy (AET), and antibiotic definitive therapy (ADT), respectively. Conclusions: NM caused by MDRE is an important sign of the failure of neurosurgery. MDRE possesses multiple drug resistance genotypes, and EVD and a GCS score ≤;8 are independent mortality risk factors for patients with MDRE-NM, which deserve the attention of microbiologists and neurosurgical clinicians. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
5. Clinical Feature, Therapy, Antimicrobial Resistance Gene Distribution, and Outcome of Nosocomial Meningitis Induced by Multidrug-Resistant Enterobacteriaceae—A Longitudinal Cohort Study From Two Neurosurgical Centers in Northern China
- Author
-
Guanghui Zheng, Yijun Shi, Yanfei Cao, Lingye Qian, Hong Lv, Lina Zhang, and Guojun Zhang
- Subjects
MDRE ,nosocomial meningitis ,antimicrobial resistance genes ,clinical feature ,outcome ,Microbiology ,QR1-502 - Abstract
ObjectivesThis is a comparative cohort study aiming to evaluate the mortality risk factors for patients with nosocomial meningitis (NM) induced by multidrug-resistant Enterobacteriaceae (MDRE) in China. The clinical features and therapies of patients and the resistance mechanisms of MDRE pathogens were also assessed.MethodsMDRE-NM patients from two neurosurgical centers in China from 2014 to 2019 were included in this study. Clinical features were extracted from the medical record databases of the two centers. The molecular mechanisms underlying the microbiological resistance mechanisms of each MDRE pathogen were determined, Kaplan–Meier survival analysis was conducted, and multivariable analyses were performed using a Cox proportional hazard model.ResultsNinety MDRE-NM patients were included in this study. Klebsiella pneumoniae accounted for the highest proportion of causative pathogens (46/90, 51.1%), and 40 causative pathogens (44.4%) were meropenem-resistant. blaKPC (27/40, 67.5%) was the predominant carbapenem resistance gene. Multivariate Cox analysis showed that external ventricular drainage (EVD) [hazard ratio (HR) = 2.524, 95% confidence interval (CI) = 1.101–5.787, p = 0.029] and a Glasgow Coma Scale (GCS) score ≤;8 (HR = 4.033, 95% CI = 1.526–10.645, p = 0.005) were mortality risk factors for patients with MDRE-NM. A total of 90.0%, 94.4%, and 97.8% of MDRE-NM patients received antibiotic prophylaxis (AP), antibiotic empirical therapy (AET), and antibiotic definitive therapy (ADT), respectively.ConclusionsNM caused by MDRE is an important sign of the failure of neurosurgery. MDRE possesses multiple drug resistance genotypes, and EVD and a GCS score ≤;8 are independent mortality risk factors for patients with MDRE-NM, which deserve the attention of microbiologists and neurosurgical clinicians.
- Published
- 2022
- Full Text
- View/download PDF
6. Trends in Occurrence and Phenotypic Resistance of Coagulase-Negative Staphylococci (CoNS) Found in Human Blood in the Northern Netherlands between 2013 and 2019
- Author
-
Matthijs S. Berends, Christian F. Luz, Alewijn Ott, Gunnar I. Andriesse, Karsten Becker, Corinna Glasner, and Alex W. Friedrich
- Subjects
coagulase-negative staphylococci ,AMR ,antibiotic resistance ,Staphylococcus ,MDRE ,Staphylococcus haemolyticus ,Biology (General) ,QH301-705.5 - Abstract
Background: For years, coagulase-negative staphylococci (CoNS) were not considered a cause of bloodstream infections (BSIs) and were often regarded as contamination. However, the association of CoNS with nosocomial infections is increasingly recognized. The identification of more than 40 different CoNS species has been driven by the introduction of matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Yet, treatment guidelines consider CoNS as a whole group, despite increasing antibiotic resistance (ABR) in CoNS. This retrospective study provides an in-depth data analysis of CoNS isolates found in human blood culture isolates between 2013 and 2019 in the entire region of the Northern Netherlands. Methods: In total, 10,796 patients were included that were hospitalized in one of the 15 hospitals in the region, leading to 14,992 CoNS isolates for (ABR) data analysis. CoNS accounted for 27.6% of all available 71,632 blood culture isolates. EUCAST Expert rules were applied to correct for errors in antibiotic test results. Results: A total of 27 different CoNS species were found. Major differences were observed in occurrence and ABR profiles. The top five species covered 97.1% of all included isolates: S. epidermidis, S. hominis, S. capitis, S. haemolyticus, and S. warneri. Regarding ABR, methicillin resistance was most frequently detected in S. haemolyticus (72%), S. cohnii (65%), and S. epidermidis (62%). S. epidermidis and S. haemolyticus showed 50–80% resistance to teicoplanin and macrolides while resistance to these agents remained lower than 10% in most other CoNS species. Conclusion: These differences are often neglected in national guideline development, prompting a focus on ‘ABR-safe’ agents such as glycopeptides. In conclusion, this multi-year, full-region approach to extensively assess the trends in both the occurrence and phenotypic resistance of CoNS species could be used for evaluating treatment policies and understanding more about these important but still too often neglected pathogens.
- Published
- 2022
- Full Text
- View/download PDF
7. Application of the mode-shape expansion based on model order reduction methods to a composite structure
- Author
-
Fuentes Humberto Peredo
- Subjects
composites ,mode-shape expansion ,guyan ,modal ,serep ,dynamic ,mdre ,mdre-we ,mac ,k-mac ,m-mac ,Engineering (General). Civil engineering (General) ,TA1-2040 - Abstract
The application of different mode-shape expansion (MSE) methods to a CFRP based on model order reduction (MOR) and component mode synthesis (CMS) methods is evaluated combining the updated stiffness parameters of the full FE model obtained with a mix-numerical experimental technique (MNET) in a previous work. The eigenvectors and eigenfrequencies of the different MSE methods obtained are compared with respect to the experimental measurements and with a full FE model solutions using the modal assurance criteria (MAC). Furthermore, the stiffness and mass weighted coefficients (K-MAC and M-MAC respectively) are calculated and compared to observe the influence of the different subspace based expansion methods applying the MAC criteria. The K-MAC and M-MAC are basically the MAC coefficients weighted by a partition of the global stiffness and mass matrices respectively. The best K-MAC and M-MAC results per paired mode-sensor are observed in the subspace based expansion MODAL/SEREP and MDRE-WE methods using the updated stiffness parameters. A strong influence of the subspace based on MOR using MSE methods is observed in the K-MAC and M-MAC criteria implemented in SDTools evaluating the stiffness parameters in a contrieved example.
- Published
- 2017
- Full Text
- View/download PDF
8. Model-Based Development of Medical Devices
- Author
-
Becker, Uwe, Hutchison, David, Series editor, Kanade, Takeo, Series editor, Kittler, Josef, Series editor, Kleinberg, Jon M., Series editor, Mattern, Friedemann, Series editor, Mitchell, John C., Series editor, Naor, Moni, Series editor, Nierstrasz, Oscar, Series editor, Pandu Rangan, C., Series editor, Steffen, Bernhard, Series editor, Sudan, Madhu, Series editor, Terzopoulos, Demetri, Series editor, Tygar, Doug, Series editor, Vardi, Moshe Y., Series editor, Weikum, Gerhard, Series editor, Buth, Bettina, editor, Rabe, Gerd, editor, and Seyfarth, Till, editor
- Published
- 2009
- Full Text
- View/download PDF
9. Improving Model Reviewing and Experimentation with Tool Support: A Controlled Experiment
- Author
-
Kretz, Dominik
- Subjects
Review Administration ,Model Review and Experiment Administration Tool-Support ,MDRE ,Experiment Administration ,Model Design and Review Editor ,Model Review ,Experimentation - Abstract
Um Fehler in Softwaresystemen zu finden werden Modelle verwendet. Diese Modelle sind eine vereinfachte Darstellung des realen Softwaresystems und k��nnen mit Hilfe von Modell-Reviews untersucht werden. F��r den Entwurf und die ��berpr��fung verschiedener Modelle gibt es jedoch nur begrenzte Software-Unterst��tzung.H��ufig werden einzelne Review-Typen, Software-Unterst��tzungen oder allgemeine Methoden innerhalb einer wissenschaftlichen Studie verglichen. Die Verwaltung von Reviews und Experimenten ist jedoch eine ressourcenintensive Aufgabe. Daher ist es unser Ziel die derzeit begrenzte Software-Unterst��tzung f��r Modell-Reviews und die Verwaltung von Reviews und Experimenten weiterzuentwickeln. Wir verwenden den Model Design and Review Editor (MDRE) und erweitern diesen mit zwei Prototyp-Komponenten. Die erste Komponente bietet Software-Unterst��tzung f��r die Meldung von Fehlern innerhalb von Modell-Reviews. Dadurch wird die Durchf��hrung des Reviews verbessert und der Aufwand f��r die Datenerfassung reduziert. Zus��tzlich implementiert die zweite Komponente eine Software-Unterst��tzung f��r die Verwaltung von Reviews und Experimenten. Das Hauptziel dieser zweiten Komponente ist die Bereitstellung von Aufgaben und die ��berwachung des Fortschritts w��hrend des Experiments, um den Verwaltungsaufwand zu reduzieren.Zur Evaluierung unserer Ergebnisse f��hren wir ein kontrolliertes Experiment mit ca. 80 Teilnehmern durch, in dem wir die MDRE-Toolunterst��tzung mit dem traditionellen Ansatz vergleichen und die Ergebnisse analysieren. Im Rahmen dieses Experiments vergleichen wir den traditionellen Ansatz mit dem MDRE. Die Auswertung zeigt, dass die in den MDRE implementierten Erweiterungen in allen gemessenen Kategorien ��hnliche Ergebnisse liefern wie der herk��mmliche Ansatz, sowohl bei Modell-Reviews als auch bei der Verwaltung von Experimenten und Reviews. Der MDRE ist also in der Lage, Reviews durchzuf��hren, die mit dem traditionellen Review-Ansatz vergleichbar sind. Dar��ber hinaus reduziert die implementierte Software-Unterst��tzung den Aufwand und vereinfacht den Prozess., Models are used to find defects in software systems. These models are a simplified view of the real software system and can be inspected using model reviews to identify defects within them. However, for the design and review of different models, there is only limited tool support available. It is often important to compare individual review types, tool support components, or general methods within a scientific study like an experiment. However, managing reviews and experiments is a laborious task. Therefore, in this work, we aim to advance the currently limited tool support for model reviews and the administration of reviews and experiments. We use the Model Design and Review Editor (MDRE) in its initial state and aim to extend it with two tool support prototypes. The first component provides tool support for reporting defects within model reviews. This improves the review execution and reduces the effort for data collection. Additionally, the second component implements tool support for administrating reviews and experiments. The main goal of this second component is the provision of tasks to the experiment participants and the monitoring of their progress during the experiment. For the evaluation of our results, we conduct a controlled experiment in which we compare the advanced MDRE tool support with the traditional pen-and-paper-based approach. The evaluation shows that the enhancements implemented into the MDRE provide similar results compared to the traditional pen-and-paper-based approach in all measured categories for both model reviews and the administration of experiments and reviews. Therefore, the improved state of the MDRE is capable of performing reviews similar to the traditional pen-and-paper inspection process. Furthermore, the implemented tool support for the administration of reviews and experiments reduces the effort and simplifies the process.
- Published
- 2021
- Full Text
- View/download PDF
10. DYSF promotes monocyte activation in atherosclerotic cardiovascular disease as a DNA methylation-driven gene.
- Author
-
Zhang X, He D, Xiang Y, Wang C, Liang B, Li B, Qi D, Deng Q, Yu H, Lu Z, and Zheng F
- Subjects
- Animals, Apolipoproteins E metabolism, Humans, Mice, Monocytes metabolism, Atherosclerosis genetics, Atherosclerosis metabolism, Cardiovascular Diseases metabolism, DNA Methylation, Dysferlin genetics, Dysferlin metabolism
- Abstract
Dysferlin (DYSF) has drawn much attention due to its involvement in dysferlinopathy and was reported to affect monocyte functions in recent studies. However, the role of DYSF in the pathogenesis of atherosclerotic cardiovascular diseases (ASCVD) and the regulation mechanism of DYSF expression have not been fully studied. In this study, Gene Expression Omnibus (GEO) database and epigenome-wide association study (EWAS) literatures were searched to find the DNA methylation-driven genes (including DYSF) of ASCVD. The hub genes related to DYSF were also identified through weighted correlation network analysis (WGCNA). Regulation of DYSF expression through its promoter methylation status was verified using peripheral blood leucocytes (PBLs) from ASCVD patients and normal controls, and experiments on THP1 cells and Apoe
-/- mice. Similarly, the expressions of DYSF related hub genes, mainly contained SELL, STAT3 and TMX1, were also validated. DYSF functions were then evaluated by phagocytosis, transwell and adhesion assays in DYSF knock-down and overexpressed THP1 cells. The results showed that DYSF promoter hypermethylation up-regulated its expression in clinical samples, THP1 cells and Apoe-/- mice, confirming DYSF as a DNA methylation-driven gene. The combination of DYSF expression and methylation status in PBLs had a considerable prediction value for ASCVD. Besides, DYSF could enhance the phagocytosis, migration and adhesion ability of THP1 cells. Among DYSF related hub genes, SELL was proven to be the downstream target of DYSF by wet experiments. In conclusion, DYSF promoter hypermethylation upregulated its expression and promoted monocytes activation, which further participated in the pathogenesis of ASCVD., (Copyright © 2022 Elsevier Inc. All rights reserved.)- Published
- 2022
- Full Text
- View/download PDF
11. Prevalence of vancomycin resistance and multiple drug resistance in enterococci in equids in North India.
- Author
-
Singh, Bhoj Raj
- Subjects
- *
VANCOMYCIN resistance , *EQUIDAE , *ENTEROCOCCAL infections , *DISEASE prevalence , *PATHOGENIC microorganisms , *DISEASES , *THERAPEUTICS - Abstract
Introduction: Vancomycin resistant and multi-drug-resistant enterococci are the major emerging pathogens in surgical, neonatal, and tertiary care units. Methodology: In this study, 267 enterococci from different clinical and non-clinical samples of equine origin were tested for their antimicrobial drug sensitivity against 19 antimicrobials using disc diffusion method. Results: A total of 80.2% enterococci tested were resistant to vancomycin and 99.6% to multiple-drugs. There was a significant association between haemolytic potential and vancomycin resistance (χ², 0.00). Enterococci isolates from healthy equids were significantly (χ², 0.04) less resistant to vancomycin than the isolates from clinically sick animals. Besides vancomycin sensitivity, isolates were also tested for 18 more antimicrobial drugs; maximum numbers of isolates were sensitive to imipenem (75%) followed by tetracycline (60%), amoxicillin+clavulanic acid (54%), and minimum for cefdinir (4%). Conclusion: More than 80% strains of enterococci of equine origin were found resistant to vancomycin and 99.6% were multiple-drug resistant in Northern India. High prevalence of VRE and MDRE in healthy equids might be a potential danger for the health of persons in equine contact. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
12. Application of the mode-shape expansion based on model order reduction methods to a composite structure
- Author
-
Humberto Peredo Fuentes
- Subjects
m-mac ,Environmental Engineering ,Materials science ,mac ,Aerospace Engineering ,02 engineering and technology ,composites ,01 natural sciences ,serep ,Composite structure ,0203 mechanical engineering ,mode-shape expansion ,mdre ,Computer Science::Networking and Internet Architecture ,ddc:531 ,General Materials Science ,0101 mathematics ,Electrical and Electronic Engineering ,Composite material ,guyan ,Computer Science::Information Theory ,Civil and Structural Engineering ,Model order reduction ,dynamic ,ddc:518 ,Mechanical Engineering ,modal ,518 Numerische Analysis ,k-mac ,Engineering (General). Civil engineering (General) ,531 Klassische Mechanik, Festkörpermechanik ,010101 applied mathematics ,020303 mechanical engineering & transports ,Modal ,mdre-we ,TA1-2040 - Abstract
The application of different mode-shape expansion (MSE) methods to a CFRP based on model order reduction (MOR) and component mode synthesis (CMS) methods is evaluated combining the updated stiffness parameters of the full FE model obtained with a mix-numerical experimental technique (MNET) in a previous work. The eigenvectors and eigenfrequencies of the different MSE methods obtained are compared with respect to the experimental measurements and with a full FE model solutions using the modal assurance criteria (MAC). Furthermore, the stiffness and mass weighted coefficients (K-MAC and M-MAC respectively) are calculated and compared to observe the influence of the different subspace based expansion methods applying the MAC criteria. The K-MAC and M-MAC are basically the MAC coefficients weighted by a partition of the global stiffness and mass matrices respectively. The best K-MAC and M-MAC results per paired mode-sensor are observed in the subspace based expansion MODAL/SEREP and MDRE-WE methods using the updated stiffness parameters. A strong influence of the subspace based on MOR using MSE methods is observed in the K-MAC and M-MAC criteria implemented in SDTools evaluating the stiffness parameters in a contrieved example.
- Published
- 2017
13. Towards model driven reverse engineering to UML behaviors - from C# code to fUML models
- Author
-
Lackner, Martin
- Subjects
MDE ,MDRE ,fUML ,C# - Abstract
Model Driven Engineering (MDE) zielt darauf ab die Abstraktionsebene in der Softwareentwicklung durch die Verwendung von Modellen zu erh��hen. MDE Techniken k��nnen sowohl zur Erstellung neuer Softwaresysteme verwendet werden, als auch um bestehende Softwaresysteme zu modernisieren oder erweitern. Die letztere Anwendung erfordert das Reverse Engineering (RE) von bestehenden Softwaresystemen zu abstrakteren Modellen. Das Hauptziel von RE ist es, relevante Informationen ��ber bestehende Software zu extrahieren und in abstraktere Modelle f��r Analysezwecke abzubilden. Model Driven Reverse Engineering (MDRE) ist die Anwendung von MDE Techniken f��r RE Aufgaben. W��hrend bereits viele Ans��tze existieren um strukturelle Informationen ��ber bestehende Systemen mittels RE in Modellen abzubilden, gibt es bis heute kaum RE Ans��tze, die detaillierte Verhaltensbeschreibungen von bestehenden Systemen extrahieren und abbilden. Im Zuge dieser Diplomarbeit wurde ein RE Ansatz erarbeitet, welcher detaillierte Verhaltensmodelle erstellt. Das Ziel dieser Arbeit ist es, ein Mapping zwischen Softwaresystemen, welche in der Programmiersprache C# programmiert sind, und UML Modellen, welche dem fUML Standard entsprechen, zu erarbeiten. Um das Verhalten von bestehenden Softwaresystemen zu beschreiben, wurde der OMG Standard Semantics of a Foundational Subset for Executable UML Models, oder foundational UML (fUML) gew��hlt, da man mit fUML Modellen das Verhalten von Softwaresystemen pr��zise und komplett beschreiben kann. Somit ist fUML ein geeigneter Kandidat als Zielsprache f��r einen MDRE Ansatz, welcher das detailierte Verhalten von Softwaresystemen abbilden soll. Im Zuge dieser Arbeit wurde auch ein Prototyp entwickelt, welcher in der Lage ist, Softwaresysteme, die in C# programmiert wurden, zu fUML konformen Modellen zu transformieren, und diese Modelle in der UML Modellierungsumgebung Enterprise Architekt zu persistieren., Model Driven Engineering (MDE) aims to raise the level of abstraction in software engineering by moving from code-centric approaches to model-centric ones, which means that the main artifacts in the software development process are models. Thereby, MDE can be used for both creating new software as well as modernizing or extending existing software. The latter usage scenario of MDE requires the reverse engineering (RE) of existing software into higher-level models. The main aim of RE is to extract information of existing software and obtain a more abstract view for further analysis. Model Driven Reverse Engineering (MDRE) is the application of MDE techniques to perform RE tasks. While many MDRE approaches for reverse engineering the structure of a system already exist, there is a lack of approaches for also reverse engineering the behavior of a system, especially detailed behavior descriptions including algorithmic details of the software. This work proposes an approach for overcoming this gap by using MDE techniques to reverse engineer the detailed behavior of a system. The goal of this work is to elaborate a mapping between code written in the general purpose programming language C# and UML models conformant to fUML, by using an MDRE approach. The OMG standard Semantics of a Foundational Subset for Executable UML Models or foundational UML (fUML) is chosen because it is possible to precisely and completely define the behavior of a software system with fUML models. Thus, fUML is a suitable candidate for serving as target language for MDRE approaches that aim to reverse engineer the detailed behavior of a software system. A prototype has been developed in this thesis, which is able to reverse engineer code written in C# to models conformant to fUML, and store them in the UML modeling environment Enterprise Architect.
- Published
- 2017
- Full Text
- View/download PDF
14. Hacia la modernización de aplicaciones corporativas dirigidas por modelos de datos. Implementación de un round-trip multidimensional orientado a aspectos JEE
- Author
-
García Pau, Víctor
- Subjects
Mdre ,Adm ,Máster Universitario en Ingeniería del Software, Métodos Formales y Sistemas de Información-Màster Universitari en Enginyeria del Programari, Mètodes Formals i Sistemes D'Informació ,Aspect slicing ,LENGUAJES Y SISTEMAS INFORMATICOS - Abstract
Este trabajo ha estudiado, en el contexto de la evolución de software, como resolver el problema de la sincronización parcial de dimensiones definidas por los aspectos de una aplicación JEE anotada, desde un enfoque dirigido por modelos.
- Published
- 2011
15. Hacia la modernización de aplicaciones corporativas dirigidas por modelos de datos. Implementación de un round-trip multidimensional orientado a aspectos JEE
- Author
-
Carsí Cubel, José Ángel, Cabot, Jordi, Universitat Politècnica de València. Servicio de Alumnado - Servei d'Alumnat, García Pau, Víctor, Carsí Cubel, José Ángel, Cabot, Jordi, Universitat Politècnica de València. Servicio de Alumnado - Servei d'Alumnat, and García Pau, Víctor
- Abstract
Este trabajo ha estudiado, en el contexto de la evolución de software, como resolver el problema de la sincronización parcial de dimensiones definidas por los aspectos de una aplicación JEE anotada, desde un enfoque dirigido por modelos.
- Published
- 2012
16. ModelExtractor: an Automatic Parametric Model Extractor
- Author
-
Bézivin, Jean, Chevrel, Régis, Bruneliere, Hugo, Jossic, Albin, Piers, William, Jouault, Frédéric, Complex data management in distributed systems (ATLAS), Université de Nantes (UN)-Inria Rennes – Bretagne Atlantique, and Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)
- Subjects
Parametric metamodel ,MDRE ,[INFO.INFO-SE]Computer Science [cs]/Software Engineering [cs.SE] ,dynamic model ,[INFO.INFO-CL]Computer Science [cs]/Computation and Language [cs.CL] ,tagged metamodel - Abstract
International audience; Building on previous experiments of reverse engineering with the Squeak language, we have designed a new prototype on the DotNet platform that should be able to exploit the full capabilities of the Visual Basic 9.0 language, including the facilities offered for dynamic typing. The objective of this work is to prove that we are able to implement an automatic model mining facility when the system consists of a legacy with good reflectivity capabilities. The initial work presented in this paper comforts the previous results obtained for Squeak and constitute the basis of a reverse engineering development toolkit that is intended to be extended in the coming months.
- Published
- 2006
17. Requirements Engineering Supporting Technical Product Management
- Author
-
Gorschek, Tony
- Subjects
Requirements Engineering ,Market-driven requirements engineering ,RAM ,Software engineering ,Programvaruteknik ,MDRE ,SPI ,Industry relevant research ,RE ,Product Management ,Software Process Assessment and Improvement ,Requirements abstraction model - Abstract
Market-Driven Requirements Engineering (MDRE) handles the continuous flow of requirements in an engineering effort, and is not limited to a development instance but part of technical product management as a whole. The market-driven environment generates large amounts of requirements from multiple sources, internal and external, threatening to overload the technical management of products. MDRE involves requirements analysis, resource estimation, prioritization, and ultimately release planning. These activities allow for effectively deciding which customers get what features and quality at what point in time, making the accuracy and efficiency of MDRE a major determinant of the success of a product. This thesis presents research aimed at supporting technical product management in MDRE, based on needs identified in industry. One contribution of the thesis is the development and validation of a process assessment and improvement planning framework, making the identification of process improvement issues possible. The major characteristics of the framework can be described as resource efficiency and relative accuracy by utilizing multiple perspectives and data point triangulation. As a complement to the assessment, the improvement planning enables industry professionals to focus their efforts on one improvement package at a time, supporting step-by-step evolution with less time to return on investment. Challenges identified during process assessment using the framework resulted in the development of the Requirements Abstraction Model (RAM), which is the central contribution of the thesis. RAM employs multiple levels of abstraction for requirements elicitation, analysis, refinement and management. The model offers the possibility for early requirements triage using product strategies/roadmaps, as well as supporting a structured and repeatable way to perform continuous requirements engineering. This enables product managers to specify requirements that can be traced across abstraction levels, from abstract requirements that can be used by managers, to refined requirements specific enough to be used for estimation and input to development efforts. RAM was engineered based on industry needs, validated and refined through several empirical evaluations, utilizing both academia and industry as laboratory. This involved close collaboration with industrial partners, Danaher Motion Särö AB and ABB Robotics, where the model was introduced. Monitoring the process improvement (introduction of RAM) indicated substantial improvements, both in accuracy of the work performed using RAM, and in the quality of the requirements produced, with only moderate increase in effort. Another contribution in the thesis is a compilation of lessons learned from practical handson experience of technology and knowledge transfer from academia to industry, with focus of producing industry relevant usable and useful results. The main contribution of the thesis is improving the practice of product management by providing and evaluating frameworks for software process improvement and market-driven requirements engineering. The results of the research presented in the thesis are aimed at supporting technical product management taking the unique challenges of market-driven development into account.
- Published
- 2006
18. Functional analysis of BpDREB2 gene involved in salt and drought response from a woody plant Broussonetia papyrifera.
- Author
-
Sun J, Peng X, Fan W, Tang M, Liu J, and Shen S
- Subjects
- Amino Acid Sequence, Arabidopsis genetics, Arabidopsis metabolism, Base Sequence, Broussonetia classification, Cold Temperature, DNA, Complementary chemistry, DNA, Complementary genetics, Gene Expression Regulation, Plant, Gene Order, Genetic Vectors genetics, Intracellular Space metabolism, Molecular Sequence Data, Organ Specificity genetics, Phenotype, Phylogeny, Plant Proteins genetics, Plant Proteins metabolism, Plants, Genetically Modified, Protein Binding, Protein Transport, Response Elements, Sequence Alignment, Stress, Physiological genetics, Transcriptional Activation, Two-Hybrid System Techniques, Broussonetia genetics, Broussonetia metabolism, Droughts, Salt Tolerance genetics, Transcription Factors genetics, Transcription Factors metabolism
- Abstract
The dehydration-responsive element binding proteins (DREBs) are important transcription factors in the regulation of plant responses to abiotic stresses. In this study, BpDREB2, an AP2/DREB-type transcription factor gene, was cloned from a woody plant, Broussonetia papyrifera by RACE-PCR. Sequence analyses revealed that BpDREB2 protein has three characteristic domains, including an AP2/EREBP, a nuclear localization signal and an acidic activation domain. Yeast one-hybrid assays showed that BpDREB2 protein specifically binds to the DRE sequence and activates the expression of reporter genes in yeast. These results suggested that BpDREB2 protein could function as a transcription factor of DREB family. The expression of BpDREB2 gene was remarkably induced by dehydration and high-salt treatments, but no significant change was observed under ABA or low-temperature conditions. Importantly, transgenic expression of BpDREB2 gene in Arabidopsis significantly enhanced its tolerance to salt and freezing without causing growth retardation. Taken together, these results suggested that BpDREB2 is a novel member of the AP2/EREBP trans-acting factor family which could enhance salt stress tolerance of plants and has the potential application in the improvement of crops and economical tree species., (Copyright © 2013 Elsevier B.V. All rights reserved.)
- Published
- 2014
- Full Text
- View/download PDF
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