Author: "MARTINS,ANTÔNIO ROBERTO" - Searchworks@Jio Institute Digital Library Search Results

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Author: Calábria, Luciana Karen, Peixoto, Pablo Marco Veras, Passos Lima, Andreia Barcelos, Peixoto, Leonardo Gomes, de Moraes, Viviane Rodrigues Alves, Teixeira, Renata Roland, dos Santos, Claudia Tavares, e Silva, Letícia Oliveira, da Silva, Maria de Fátima Rodrigues, dos Santos, Ana Alice Diniz, Garcia-Cairasco, Norberto, Martins, Antônio Roberto, Espreafico, Enilza Maria, and Espindola, Foued Salmen
Published: 2011
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Author: Weinman, Angela Regina Maciel, Jorge, Salim Moysés, Martins, Antonio Roberto, de Assis, Maria das Graças Elias, Martinez, Francisco Eulógio, and Camelo, José Simon, Jr.
Published: 2007
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Author: Ferreira, Heloisa Helena de Araujo, Costa, Rosana Aparecida de Oliveira, Jacheta, Jerusa Maria, Martins, Antonio Roberto, Medeiros, Marta Valéria, Macedo-Soares, Maria Fernanda, De Luca, Iara Maria Silva, Antunes, Edson, and De Nucci, Gilberto
Published: 2004
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Author: CALÁBRIA,LUCIANA KAREN, TEIXEIRA,RENATA ROLAND, COELHO GONÇALVES,SYBELLI MAGDA, PASSOS LIMA,ANDREIA BARCELOS, SANTOS,ANA ALICE DINIZ DOS, MARTINS,ANTÔNIO ROBERTO, ESPINDOLA,FOUED SALMEN, CALÁBRIA,LUCIANA KAREN, TEIXEIRA,RENATA ROLAND, COELHO GONÇALVES,SYBELLI MAGDA, PASSOS LIMA,ANDREIA BARCELOS, SANTOS,ANA ALICE DINIZ DOS, MARTINS,ANTÔNIO ROBERTO, and ESPINDOLA,FOUED SALMEN
Abstract: The present study compared two heating methods currently used for antigen retrieval (AR) immunostaining: the microwave oven and the steam cooker. Myosin-V, a molecular motor involved in vesicle transport, was used as a neuronal marker in honeybee Apis mellifera brains fixed in formalin. Overall, the steam cooker showed the most satisfactory AR results. At 100 ºC, tissue morphology was maintained and revealed epitope recovery, while evaporation of the AR solution was markedly reduced; this is important for stabilizing the sodium citrate molarity of the AR buffer and reducing background effects. Standardization of heat-mediated AR of formalin-fixed and paraffin-embedded tissue sections results in more reliable immunostaining of the honeybee brain.
Published: 2010

Author: CALÁBRIA, LUCIANA KAREN, primary, TEIXEIRA, RENATA ROLAND, additional, COELHO GONÇALVES, SYBELLI MAGDA, additional, PASSOS LIMA, ANDREIA BARCELOS, additional, SANTOS, ANA ALICE DINIZ DOS, additional, MARTINS, ANTÔNIO ROBERTO, additional, and ESPINDOLA, FOUED SALMEN, additional
Published: 2010
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