124 results on '"MARTA NOVO"'
Search Results
2. Transcriptome analysis of aphids exposed to glandular trichomes in tomato reveals stress and starvation related responses
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Rosario Planelló, Lola Llorente, Óscar Herrero, Marta Novo, Lidia Blanco-Sánchez, Juan Antonio Díaz-Pendón, Rafael Fernández-Muñoz, Victoria Ferrero, and Eduardo de la Peña
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Medicine ,Science - Abstract
Abstract Understanding the responses of insect herbivores to plant chemical defences is pivotal for the management of crops and pests. However, the mechanisms of interaction are not entirely understood. In this study, we compared the whole transcriptome gene expression of the aphid Macrosiphum euphorbiae grown on two different varieties of tomato that differ in their inducible chemical defences. We used two isogenic lines of tomato with a shared genetic background that only differ in the presence of type IV glandular trichomes and their associated acylsucrose excretions. This works also reports a de novo transcriptome of the aphid M. euphorbiae. Subsequently, we identified a unique and distinct gene expression profile for the first time corresponding to aphid´s exposure to type IV glandular trichomes and acylsugars. The analysis of the aphid transcriptome shows that tomato glandular trichomes and their associated secretions are highly efficient in triggering stress-related responses in the aphid, and demonstrating that their role in plant defence goes beyond the physical impediment of herbivore activity. Some of the differentially expressed genes were associated with carbohydrate, lipid and xenobiotic metabolisms, immune system, oxidative stress response and hormone biosynthesis pathways. Also, the observed responses are compatible with a starvation syndrome. The transcriptome analysis puts forward a wide range of genes involved in the synthesis and regulation of detoxification enzymes that reveal important underlying mechanisms in the interaction of the aphid with its host plant and provides a valuable genomic resource for future study of biological processes at the molecular level using this aphid.
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- 2022
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3. Global data on earthworm abundance, biomass, diversity and corresponding environmental properties
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Helen R. P. Phillips, Elizabeth M. Bach, Marie L. C. Bartz, Joanne M. Bennett, Rémy Beugnon, Maria J. I. Briones, George G. Brown, Olga Ferlian, Konstantin B. Gongalsky, Carlos A. Guerra, Birgitta König-Ries, Julia J. Krebs, Alberto Orgiazzi, Kelly S. Ramirez, David J. Russell, Benjamin Schwarz, Diana H. Wall, Ulrich Brose, Thibaud Decaëns, Patrick Lavelle, Michel Loreau, Jérôme Mathieu, Christian Mulder, Wim H. van der Putten, Matthias C. Rillig, Madhav P. Thakur, Franciska T. de Vries, David A. Wardle, Christian Ammer, Sabine Ammer, Miwa Arai, Fredrick O. Ayuke, Geoff H. Baker, Dilmar Baretta, Dietmar Barkusky, Robin Beauséjour, Jose C. Bedano, Klaus Birkhofer, Eric Blanchart, Bernd Blossey, Thomas Bolger, Robert L. Bradley, Michel Brossard, James C. Burtis, Yvan Capowiez, Timothy R. Cavagnaro, Amy Choi, Julia Clause, Daniel Cluzeau, Anja Coors, Felicity V. Crotty, Jasmine M. Crumsey, Andrea Dávalos, Darío J. Díaz Cosín, Annise M. Dobson, Anahí Domínguez, Andrés Esteban Duhour, Nick van Eekeren, Christoph Emmerling, Liliana B. Falco, Rosa Fernández, Steven J. Fonte, Carlos Fragoso, André L. C. Franco, Abegail Fusilero, Anna P. Geraskina, Shaieste Gholami, Grizelle González, Michael J. Gundale, Mónica Gutiérrez López, Branimir K. Hackenberger, Davorka K. Hackenberger, Luis M. Hernández, Jeff R. Hirth, Takuo Hishi, Andrew R. Holdsworth, Martin Holmstrup, Kristine N. Hopfensperger, Esperanza Huerta Lwanga, Veikko Huhta, Tunsisa T. Hurisso, Basil V. Iannone, Madalina Iordache, Ulrich Irmler, Mari Ivask, Juan B. Jesús, Jodi L. Johnson-Maynard, Monika Joschko, Nobuhiro Kaneko, Radoslava Kanianska, Aidan M. Keith, Maria L. Kernecker, Armand W. Koné, Yahya Kooch, Sanna T. Kukkonen, H. Lalthanzara, Daniel R. Lammel, Iurii M. Lebedev, Edith Le Cadre, Noa K. Lincoln, Danilo López-Hernández, Scott R. Loss, Raphael Marichal, Radim Matula, Yukio Minamiya, Jan Hendrik Moos, Gerardo Moreno, Alejandro Morón-Ríos, Hasegawa Motohiro, Bart Muys, Johan Neirynck, Lindsey Norgrove, Marta Novo, Visa Nuutinen, Victoria Nuzzo, P. Mujeeb Rahman, Johan Pansu, Shishir Paudel, Guénola Pérès, Lorenzo Pérez-Camacho, Jean-François Ponge, Jörg Prietzel, Irina B. Rapoport, Muhammad Imtiaz Rashid, Salvador Rebollo, Miguel Á. Rodríguez, Alexander M. Roth, Guillaume X. Rousseau, Anna Rozen, Ehsan Sayad, Loes van Schaik, Bryant Scharenbroch, Michael Schirrmann, Olaf Schmidt, Boris Schröder, Julia Seeber, Maxim P. Shashkov, Jaswinder Singh, Sandy M. Smith, Michael Steinwandter, Katalin Szlavecz, José Antonio Talavera, Dolores Trigo, Jiro Tsukamoto, Sheila Uribe-López, Anne W. de Valença, Iñigo Virto, Adrian A. Wackett, Matthew W. Warren, Emily R. Webster, Nathaniel H. Wehr, Joann K. Whalen, Michael B. Wironen, Volkmar Wolters, Pengfei Wu, Irina V. Zenkova, Weixin Zhang, Erin K. Cameron, and Nico Eisenhauer
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Science - Abstract
Measurement(s) earthworm communities • Abundance • organic material • Diversity • environmental properties Technology Type(s) digital curation Factor Type(s) location Sample Characteristic - Organism Lumbricina Sample Characteristic - Environment soil Sample Characteristic - Location global Machine-accessible metadata file describing the reported data: https://doi.org/10.6084/m9.figshare.13399118
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- 2021
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4. Understanding Diversity, Evolution, and Structure of Small Heat Shock Proteins in Annelida Through in Silico Analyses
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Mercedes de la Fuente and Marta Novo
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stress physiology ,small heat shock proteins ,molecular evolution ,α crystallin domain (ACD) ,dimeric architecture ,earthworms ,Physiology ,QP1-981 - Abstract
Small heat shock proteins (sHsps) are oligomeric stress proteins characterized by an α-crystallin domain (ACD). These proteins are localized in different subcellular compartments and play critical roles in the stress physiology of tissues, organs, and whole multicellular eukaryotes. They are ubiquitous proteins found in all living organisms, from bacteria to mammals, but they have never been studied in annelids. Here, a data set of 23 species spanning the annelid tree of life, including mostly transcriptomes but also two genomes, was interrogated and 228 novel putative sHsps were identified and manually curated. The analysis revealed very high protein diversity and showed that a significant number of sHsps have a particular dimeric architecture consisting of two tandemly repeated ACDs. The phylogenetic analysis distinguished three main clusters, two of them containing both monomeric sHsps, and ACDs located downstream in the dimeric sHsps, and the other one comprising the upstream ACDs from those dimeric forms. Our results support an evolutionary history of these proteins based on duplication events prior to the Spiralia split. Monomeric sHsps 76) were further divided into five subclusters. Physicochemical properties, subcellular location predictions, and sequence conservation analyses provided insights into the differentiating elements of these putative functional groups. Strikingly, three of those subclusters included sHsps with features typical of metazoans, while the other two presented characteristics resembling non-metazoan proteins. This study provides a solid background for further research on the diversity, evolution, and function in the family of the sHsps. The characterized annelid sHsps are disclosed as essential for improving our understanding of this important family of proteins and their pleotropic functions. The features and the great diversity of annelid sHsps position them as potential powerful molecular biomarkers of environmental stress for acting as prognostic tool in a diverse range of environments.
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- 2022
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5. Perspectives in Earthworm Molecular Phylogeny: Recent Advances in Lumbricoidea and Standing Questions
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Daniel Fernández Marchán, Thibaud Decaëns, Jorge Domínguez, and Marta Novo
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molecular phylogenetics ,earthworms ,systematics ,Lumbricidae ,Hormogastridae ,Biology (General) ,QH301-705.5 - Abstract
Earthworm systematics have been limited by the small number of taxonomically informative morphological characters and high levels of homoplasy in this group. However, molecular phylogenetic techniques have yielded significant improvements in earthworm taxonomy in the last 15 years. Several different approaches based on the use of different molecular markers, sequencing techniques, and compromises between specimen/taxon coverage and phylogenetic information have recently emerged (DNA barcoding, multigene phylogenetics, mitochondrial genome analysis, transcriptome analysis, targeted enrichment methods, and reduced representation techniques), providing solutions to different evolutionary questions regarding European earthworms. Molecular phylogenetics have led to significant advances being made in Lumbricidae systematics, such as the redefinition or discovery of new genera (Galiciandrilus, Compostelandrilus, Vindoboscolex, Castellodrilus), delimitation and revision of previously existing genera (Kritodrilus, Eophila, Zophoscolex, Bimastos), and changes to the status of subspecific taxa (such as the Allolobophorachaetophora complex). These approaches have enabled the identification of problems that can be resolved by molecular phylogenetics, including the revision of Aporrectodea, Allolobophora, Helodrilus, and Dendrobaena, as well as the examination of small taxa such as Perelia, Eumenescolex, and Iberoscolex. Similar advances have been made with the family Hormogastridae, in which integrative systematics have contributed to the description of several new species, including the delimitation of (formerly) cryptic species. At the family level, integrative systematics have provided a new genus system that better reflects the diversity and biogeography of these earthworms, and phylogenetic comparative methods provide insight into earthworm macroevolution. Despite these achievements, further research should be performed on the Tyrrhenian cryptic complexes, which are of special eco-evolutionary interest. These examples highlight the potential value of applying molecular phylogenetic techniques to other earthworm families, which are very diverse and occupy different terrestrial habitats across the world. The systematic implementation of such approaches should be encouraged among the different expert groups worldwide, with emphasis on collaboration and cooperation.
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- 2022
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6. The Sex-Specific Detrimental Effect of Diabetes and Gender-Related Factors on Pre-admission Medication Adherence Among Patients Hospitalized for Ischemic Heart Disease: Insights From EVA Study
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Valeria Raparelli, Marco Proietti, Giulio Francesco Romiti, Andrea Lenzi, Stefania Basili, The EVA Collaborative Group, Claudio Tiberti, Federica Panimolle, Andrea Isidori, Elisa Giannetta, Mary Anna Venneri, Laura Napoleone, Marta Novo, Silvia Quattrino, Simona Ceccarelli, Eleni Anastasiadou, Francesca Megiorni, Cinzia Marchese, Enrico Mangieri, Gaetano Tanzilli, Nicola Viceconte, Francesco Barillà, Carlo Gaudio, Vincenzo Paravati, Guglielmo Tellan, Evaristo Ettorre, Adriana Servello, Fabio Miraldi, Andrea Moretti, Alessandra Tanzilli, Piergiovanni Mazzonna, Suleyman Al Kindy, Riccardo Iorio, Martina Di Iorio, Gennaro Petriello, Laura Gioffrè, Eleonora Indolfi, Gaetano Pero, Nino Cocco, Loredana Iannetta, Sara Giannuzzi, Emilio Centaro, Sonia Cristina Sergi, Filippo Toriello, Eleonora Ruscio, Tommaso Todisco, Nicolò Sperduti, Giuseppe Santangelo, Giacomo Visioli, Marco Vano, Marco Borgi, Ludovica Maria Antonini, Silvia Robuffo, Claudia Tucci, Agostino Rossoni, Valeria Spugnardi, Annarita Vernile, Mariateresa Santoliquido, Verdiana Santori, Giulia Tosti, Fabrizio Recchia, Francesco Morricone, Roberto Scacciavillani, Alice Lipari, Andrea Zito, Floriana Testa, Giulia Ricci, Ilaria Vellucci, Marianna Vincenti, Silvia Pietropaolo, Camilla Scala, Nicolò Rubini, Marta Tomassi, Daria Amoroso, Lucia Stefanini, Simona Bartimoccia, Giovanni Talerico, Pasquale Pignatelli, Roberto Cangemi, Salvatore Minisola, Sergio Morelli, Antonio Fraioli, Silvia Nocchi, Mario Fontana, Sebastiano Filetti, Massimo Fiorilli, Danilo Toni, Anne Falcou, Louise Pilote, Tabeth Tsitsi Jiri, Muhammad Ahmer Wali, Amanpreet Kaur, Malik Elharram, Anna Rita Vestri, Patrizia Ferroni, Clara Crescioli, Cristina Antinozzi, Francesca Serena Pignataro, Tiziana Bellini, Alessandro Trentini, Roberto Carnevale, Cristina Nocella, Carlo Catalano, Iacopo Carbone, Nicola Galea, Giuliano Bertazzoni, Marianna Suppa, Antonello Rosa, Gioacchino Galardo, Maria Alessandroni, Lorena Cipollone, Alessandro Coppola, Mariangela Palladino, Giulio Illuminati, Fabrizio Consorti, Paola Mariani, Fabrizio Neri, Paolo Salis, Antonio Segatori, Laurent Tellini, and Gianluca Costabile
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sex ,gender ,diabetes ,medication adherence ,ischemic heart disease ,personality traits ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
Background: Sex and gender-related factors have been under-investigated as relevant determinants of health outcomes across non-communicable chronic diseases. Poor medication adherence results in adverse clinical outcomes and sex differences have been reported among patients at high cardiovascular risk, such as diabetics. The effect of diabetes and gender-related factors on medication adherence among women and men at high risk for ischemic heart disease (IHD) has not yet been fully investigated.Aim: To explore the role of sex, gender-related factors, and diabetes in pre-admission medication adherence among patients hospitalized for IHD.Materials and Methods: Data were obtained from the Endocrine Vascular disease Approach (EVA) (ClinicalTrials.gov Identifier: NCT02737982), a prospective cohort of patients admitted for IHD. We selected patients with baseline information regarding the presence of diabetes, cardiovascular risk factors, and gender-related variables (i.e., gender identity, gender role, gender relations, institutionalized gender). Our primary outcome was the proportion of pre-admission medication adherence defined through a self-reported questionnaire. We performed a sex-stratified analysis of clinical and gender-related factors associated with pre-admission medication adherence.Results: Two-hundred eighty patients admitted for IHD (35% women, mean age 70), were included. Around one-fourth of the patients were low-adherent to therapy before hospitalization, regardless of sex. Low-adherent patients were more likely diabetic (40%) and employed (40%). Sex-stratified analysis showed that low-adherent men were more likely to be employed (58 vs. 33%) and not primary earners (73 vs. 54%), with more masculine traits of personality, as compared with medium-high adherent men. Interestingly, women reporting medication low-adherence were similar for clinical and gender-related factors to those with medium-high adherence, except for diabetes (42 vs. 20%, p = 0.004). In a multivariate adjusted model only employed status was associated with poor medication adherence (OR 0.55, 95%CI 0.31–0.97). However, in the sex-stratified analysis, diabetes was independently associated with medication adherence only in women (OR 0.36; 95%CI 0.13–0.96), whereas a higher masculine BSRI was the only factor associated with medication adherence in men (OR 0.59, 95%CI 0.35–0.99).Conclusion: Pre-admission medication adherence is common in patients hospitalized for IHD, regardless of sex. However, patient-related factors such as diabetes, employment, and personality traits are associated with adherence in a sex-specific manner.
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- 2019
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7. Digoxin and Platelet Activation in Patients With Atrial Fibrillation: In Vivo and In Vitro Study
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Daniele Pastori, Roberto Carnevale, Cristina Nocella, Simona Bartimoccia, Marta Novo, Vittoria Cammisotto, Silvia Piconese, Maria Santulli, Fortunata Vasaturo, Francesco Violi, and Pasquale Pignatelli
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atrial fibrillation ,digoxin ,platelet aggregation ,thromboxane ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Background Digoxin use was shown to be associated with an increased risk of cardiovascular events in atrial fibrillation (AF). We hypothesized that digoxin may affect cardiovascular risk by increasing platelet activation. Methods and Results Post hoc analysis of a prospective study of anticoagulated patients with AF. Patients were divided into 2 groups balanced for age, sex, and cardiovascular risk factors: digoxin users (n=132) and nonusers (n=388). Urinary excretion of 11‐dehydro‐thromboxane B2 (TxB2), a marker of platelet activation, and serum digoxin concentration (SDC) were measured. In vitro experiments were performed on platelets from healthy subjects and AF patients, which were incubated with scalar doses of digoxin (0.6–2.4 ng/mL) with or without prestimulation with a sub‐threshold of collagen. Median 11‐dehydro‐TxB2 was 105.0 (interquartile range, 60.0–190.0) ng/mg creatinine, and median SDC was 0.65 (interquartile range, 0.40–1.00) ng/mL. Urinary 11‐dehydro‐TxB2 and SDC were correlated (rs=0.350, P
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- 2018
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8. Gut‐Derived Serum Lipopolysaccharide is Associated With Enhanced Risk of Major Adverse Cardiovascular Events in Atrial Fibrillation: Effect of Adherence to Mediterranean Diet
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Daniele Pastori, Roberto Carnevale, Cristina Nocella, Marta Novo, Maria Santulli, Vittoria Cammisotto, Danilo Menichelli, Pasquale Pignatelli, and Francesco Violi
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atrial fibrillation ,cardiovascular events ,lipopolysaccharide ,Mediterranean diet ,thromboxane ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
BackgroundGut microbiota is emerging as a novel risk factor for atherothrombosis, but the predictive role of gut‐derived lipopolysaccharide (LPS) is unknown. We analyzed (1) the association between LPS and major adverse cardiovascular events (MACE) in atrial fibrillation (AF) and (2) its relationship with adherence to a Mediterranean diet (Med‐diet). Methods and ResultsThis was a prospective single‐center study including 912 AF patients treated with vitamin K antagonists (3716 patient‐years). The primary end point was a composite of MACE. Baseline serum LPS, adherence to Med‐diet (n=704), and urinary excretion of 11‐dehydro‐thromboxane B2 (TxB2, n=852) were investigated. Mean age was 73.5 years; 42.9% were women. A total of 187 MACE (5.0% per year) occurred: 54, 59, and 74 in the first, second, and third tertile of LPS, respectively (log‐rank test P=0.004). Log‐LPS (hazard ratio 1.194, P=0.009), age (hazard ratio 1.083, P
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- 2017
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9. New light into the hormogastrid riddle: morphological and molecular description of Hormogaster joseantonioi sp. n. (Annelida, Clitellata, Hormogastridae)
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Daniel Fernández Marchán, Rosa Fernández, Marta Novo, and Dario Diaz Cosin
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Zoology ,QL1-991 - Abstract
The earthworm family Hormogastridae shows a remarkable disjunction in its distribution in the Iberian Peninsula, with the Hormogaster elisae species complex isolated from the rest of the species. Hormogaster joseantonioi sp. n., a new species found in the intermediate area between the main ranges (in Teruel, Aragón), was described following the integrative approach, as it is suitable for earthworms due to their highly homoplasic morphology. The phylogenetic analysis of the molecular markers placed the new species as a sister taxon to H. elisae, thus showing the colonizing lineage of Central Iberian Peninsula could have originated near the H. joseantonioi sp. n. current range. External morphological characters revealed some degree of overlap with previously described species, but internal characters presented configurations/states unknown from other members of the family. These traits make the new species a key piece to understand the evolution of Hormogastridae.
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- 2014
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10. A new earthworm species within a controversial genus: Eiseniona gerardoi sp. n. (Annelida, Lumbricidae) - description based on morphological and molecular data
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Dario Diaz Cosin, Marta Novo, Rosa Fernández, Daniel Fernández Marchán, and Mónica Gutiérrez
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Zoology ,QL1-991 - Abstract
The morphological and anatomical simplicity of soil dwelling animals, such as earthworms, has limited the establishment of a robust taxonomy making it sometimes subjective to authors’ criteria. Within this context, integrative approaches including molecular information are becoming more popular to solve the phylogenetic positioning of conflictive taxa. Here we present the description of a new lumbricid species from the region of Extremadura (Spain), Eiseniona gerardoi sp. n. The assignment to this genus is based on both a morphological and a phylogenetic study. The validity of the genus Eiseniona, one of the most controversial within Lumbricidae, is discussed. A synopsis of the differences between the type species and the west-European members of the genus is provided.
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- 2014
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11. Compilation of morphological and molecular data, a necessity for taxonomy: The case of Hormogaster abbatissae sp. n. (Annelida, Clitellata, Hormogastridae)
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Marta Novo, Rosa Fernández, Daniel Fernández Marchán, Mónica Gutiérrez, and Dario Diaz Cosin
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Zoology ,QL1-991 - Abstract
Conflict among data sources can be frequent in evolutionary biology, especially in cases where one character set poses limitations to resolution. Earthworm taxonomy, for example, remains a challenge because of the limited number of morphological characters taxonomically valuable. An explanation to this may be morphological convergence due to adaptation to a homogeneous habitat, resulting in high degrees of homoplasy. This sometimes impedes clear morphological diagnosis of species. Combination of morphology with molecular techniques has recently aided taxonomy in many groups difficult to delimit morphologically. Here we apply an integrative approach by combining morphological and molecular data, including also some ecological features, to describe a new earthworm species in the family Hormogastridae, Hormogaster abbatissae sp. n., collected in Sant Joan de les Abadesses (Girona, Spain). Its anatomical and morphological characters are discussed in relation to the most similar Hormogastridae species, which are not the closest species in a phylogenetic analysis of molecular data. Species delimitation using the GMYC method and genetic divergences with the closest species are also considered. The information supplied by the morphological and molecular sources is contradictory, and thus we discuss issues with species delimitation in other similar situations. Decisions should be based on a profound knowledge of the morphology of the studied group but results from molecular analyses should also be considered.
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- 2012
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12. Contribution to the knowledge of the genus Boucheona (Oligochaeta, Hormogastridae) in France with a newly described species and a redescribed species
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DANIEL F. MARCHÁN, MARTA NOVO, JORGE DOMÍNGUEZ, NURIA SÁNCHEZ, and THIBAUD DECAËNS
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Annelida ,Hormogastridae ,Crassiclitellata ,Animalia ,Clitellata ,Animal Science and Zoology ,Biodiversity ,Ecology, Evolution, Behavior and Systematics ,Taxonomy - Abstract
Hormogastrid earthworms are found in the diversity hotspot of the Franco-Iberian domain, together with the better-known family Lumbricidae. Integrative systematics (the combination of morphological, molecular and ecological data) have increased our knowledge of the diversity and evolutionary history of these earthworms, highlighting unresolved taxonomic conflicts. One example of a species group in need of integrative taxonomic revision is the genus Boucheona in France. In this work, we analyzed their diversity using previously published data together with additional data obtained from recently sampled localities. Molecular data including DNA barcodes and additional markers enabled us to reconstruct Bayesian and time-calibrated phylogenies to discuss the evolutionary relationships among the different taxa, and to propose hypotheses regarding their biogeographical history. Based on our results, four species of Boucheona are present in Southern France, including two new taxa. Morphological distinctness and molecular phylogenetics results supported the status of four populations as the newly described Boucheona corbierensis sp. nov., as well as the status of “Hormogaster pretiosa var. nigra” as an independent species, redescribed as Boucheona tenebrae sp. nov. These results provide a new perspective of the importance of the genus Boucheona in southern France, as the possible evolutionary origin of a clade of giant anecic earthworms with unknown (but probably remarkable) impact on ecosystem functioning across their range.
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- 2023
13. Phylogeography of a riparian earthworm shows environmental factors influence genetic structure
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Irene de Sosa, Daniel F. Marchán, Marta Novo, Ana Almodóvar, and Darío J. Díaz Cosín
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Ecology ,Ecology, Evolution, Behavior and Systematics - Published
- 2022
14. A strong backbone for an invertebrate group: anchored phylogenomics improves the resolution of genus-level relationships within the Lumbricidae (Annelida, Crassiclitellata)
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Daniel F. Marchán, Samuel W. James, Alan R. Lemmon, Emily Moriarty Lemmon, Marta Novo, Jorge Domínguez, Darío J. Díaz Cosín, and Dolores Trigo
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Ecology, Evolution, Behavior and Systematics - Published
- 2022
15. How genomics can help biodiversity conservation
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Kathrin Theissinger, Carlos Fernandes, Giulio Formenti, Iliana Bista, Paul R. Berg, Christoph Bleidorn, Aureliano Bombarely, Angelica Crottini, Guido R. Gallo, José A. Godoy, Sissel Jentoft, Joanna Malukiewicz, Alice Mouton, Rebekah A. Oomen, Sadye Paez, Per J. Palsbøll, Christophe Pampoulie, María J. Ruiz-López, Simona Secomandi, Hannes Svardal, Constantina Theofanopoulou, Jan de Vries, Ann-Marie Waldvogel, Guojie Zhang, Erich D. Jarvis, Miklós Bálint, Claudio Ciofi, Robert M. Waterhouse, Camila J. Mazzoni, Jacob Höglund, Sargis A. Aghayan, Tyler S. Alioto, Isabel Almudi, Nadir Alvarez, Paulo C. Alves, Isabel R. Amorim do Rosario, Agostinho Antunes, Paula Arribas, Petr Baldrian, Giorgio Bertorelle, Astrid Böhne, Andrea Bonisoli-Alquati, Ljudevit L. Boštjančić, Bastien Boussau, Catherine M. Breton, Elena Buzan, Paula F. Campos, Carlos Carreras, L. FIlipe C. Castro, Luis J. Chueca, Fedor Čiampor, Elena Conti, Robert Cook-Deegan, Daniel Croll, Mónica V. Cunha, Frédéric Delsuc, Alice B. Dennis, Dimitar Dimitrov, Rui Faria, Adrien Favre, Olivier D. Fedrigo, Rosa Fernández, Gentile Francesco Ficetola, Jean-François Flot, Toni Gabaldón, Dolores R. Agius, Alice M. Giani, M. Thomas P. Gilbert, Tine Grebenc, Katerina Guschanski, Romain Guyot, Bernhard Hausdorf, Oliver Hawlitschek, Peter D. Heintzman, Berthold Heinze, Michael Hiller, Martin Husemann, Alessio Iannucci, Iker Irisarri, Kjetill S. Jakobsen, Peter Klinga, Agnieszka Kloch, Claudius F. Kratochwil, Henrik Kusche, Kara K.S. Layton, Jennifer A. Leonard, Emmanuelle Lerat, Gianni Liti, Tereza Manousaki, Tomas Marques-Bonet, Pável Matos-Maraví, Michael Matschiner, Florian Maumus, Ann M. Mc Cartney, Shai Meiri, José Melo-Ferreira, Ximo Mengual, Michael T. Monaghan, Matteo Montagna, Robert W. Mysłajek, Marco T. Neiber, Violaine Nicolas, Marta Novo, Petar Ozretić, Ferran Palero, Lucian Pârvulescu, Marta Pascual, Octávio S. Paulo, Martina Pavlek, Cinta Pegueroles, Loïc Pellissier, Graziano Pesole, Craig R. Primmer, Ana Riesgo, Lukas Rüber, Diego Rubolini, Daniele Salvi, Ole Seehausen, Matthias Seidel, Bruno Studer, Spyros Theodoridis, Marco Thines, Lara Urban, Anti Vasemägi, Adriana Vella, Noel Vella, Sonja C. Vernes, Cristiano Vernesi, David R. Vieites, Christopher W. Wheat, Gert Wörheide, Yannick Wurm, Gabrielle Zammit, University of Zurich, Höglund, Jacob, Repositório da Universidade de Lisboa, and European Reference Genome Atlas Consortium
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genomic toolbox ,Biodiversity conservation -- Research ,Genetics, Evolution and Phylogenetics ,Settore BIO/18 - GENETICA ,udc:575.111 ,biotska pestrost ,580 Plants (Botany) ,Genomics -- Observations ,biodiversity genomics ,Genomics -- Practice -- Evaluation ,1311 Genetics ,European Reference Genome Atlas (ERGA) ,Conservation applications ,Genomic toolbox ,reference genomes ,Anthropocene ,genomics ,Genetics ,biotska pestrost, genetika ,genomics, biodiversity conservation ,10211 Zurich-Basel Plant Science Center ,Biology ,conservation applications ,Genomics -- Technological innovations ,Anthropocene biodiversity genomics genomic toolbox reference genomes conservation applications European Reference Genome Atlas (ERGA) ,Genomics -- Technique ,Biodiversity genomics ,Geference genomes ,10121 Department of Systematic and Evolutionary Botany ,genetika ,570 Life sciences ,biology ,biodiversity conservation - Abstract
The availability of public genomic resources can greatly assist biodiversity assessment, conservation, and restoration efforts by providing evidence for scientifically informed management decisions. Here we survey the main approaches and applications in biodiversity and conservation genomics, considering practical factors, such as cost, time, prerequisite skills, and current shortcomings of applications. Most approaches perform best in combination with reference genomes from the target species or closely related species. We review case studies to illustrate how reference genomes can facilitate biodiversity research and conservation across the tree of life. We conclude that the time is ripe to view reference genomes as fundamental resources and to integrate their use as a best practice in conservation genomics., Trends in Genetics, 39 (7), ISSN:0168-9525
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- 2023
16. The best is yet to come: six new species within a largebodied earthworm genus (Scherotheca, Lumbricidae) in a densely sampled region (south-western France)
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Daniel Fernández Marchán, Marta Novo, Jorge Domínguez, Alberto Da Silva, Alejandro Martínez, Sylvain Gerard, Mickaël Hedde, and Thibaud Decaëns
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Annelida ,Crassiclitellata ,Animalia ,Animal Science and Zoology ,Clitellata ,Biodiversity ,Ecology, Evolution, Behavior and Systematics ,Lumbricidae ,Taxonomy - Abstract
Since the 18th century, several taxonomists have contributed to knowledge of the French earthworm fauna, one of the best-known earthworm communities in Europe, with 164 species currently described. Surprisingly, new species keep being added constantly, even large ones, which are usually the first to be described. The recent discovery of six undescribed morphospecies of Scherotheca during a sampling survey in southern France suggests that other large Lumbricidae remain to be discovered. We provide here an integrative description of these six species, Scherotheca albina Marchán & Decaëns sp. nov., Scherotheca betharramensis Marchán & Decaëns sp. nov., Scherotheca microthomasi Marchán & Decaëns sp. nov., Scherotheca shaihulud Marchán & Decaëns sp. nov., Scherotheca etcheberryensis Marchán & Decaëns sp. nov. and Scherotheca trezencensis Marchán & Decaëns sp. nov., formally name them and place them in a molecular phylogenetic framework together with their Scherotheca relatives from south-western France. This increases the diversity of Scherotheca to 51 species, including Scherotheca haymozi comb. nov. as a small-sized representative of the genus. The validity of the elevation of several subspecies to specific status was corroborated by molecular phylogenetic evidence, and previously unknown cryptic speciation within Scherotheca was detected. Two geographically coherent clades (roughly matching Nouvelle-Aquitaine and Occitanie) correspond to two existing subgenera. The unusually high taxonomic and morphological diversity of Scherotheca within the Pyrénées-Atlantiques may be related to biotic interactions, climatic factors and historical causes. These features highlight the importance of the conservation of these unique earthworm species.
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- 2023
17. Terrestrial Zen master: Transcriptomics in long‐term aestivation and arousal of Mediterranean earthworms
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Natasha Tilikj, Alejandro Martínez Navarro, and Marta Novo
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Physiology ,Genetics ,Animal Science and Zoology ,Molecular Biology ,Ecology, Evolution, Behavior and Systematics - Published
- 2023
18. Comparative phylogeography and integrative systematic revision of Iberian endemic earthworms (Crassiclitellata, Lumbricidae)
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Sergio Jiménez Pinadero, Daniel Fernández Marchán, Alejandro Martínez Navarro, Natasha Tilikj, Marta Novo, Jorge Domínguez, Darío J. Díaz Cosín, and Dolores Trigo
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Genetics ,Animal Science and Zoology ,Molecular Biology ,Ecology, Evolution, Behavior and Systematics - Published
- 2023
19. Catch-All No More: Integrative Systematic Revision of the Genus Allolobophora Eisen, 1874 (Crassiclitellata, Lumbricidae) with the Description of Two New Relict Earthworm Genera
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Alejandro Martínez Navarro, Sergio Jiménez Pinadero, Thibaud Decaëns, Mickaël Hedde, Marta Novo, Dolores Trigo, Daniel Fernández Marchán, Universidad Complutense de Madrid = Complutense University of Madrid [Madrid] (UCM), Department of Biodiversity, Ecology and Evolution [Madrid], Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Université Paul-Valéry - Montpellier 3 (UPVM)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM), Ecologie fonctionnelle et biogéochimie des sols et des agro-écosystèmes (UMR Eco&Sols), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), subproject Fauna Ibérica XII. Oligochaeta, Lumbricoidea: Lumbricidae, Hormogastridae #PGC2018-095851-B-C66 from the Spanish Ministry of Sciences, Innovation and Universities., María Zambrano Postdoctoral Grant from the Spanish Ministry of Sciences, Innovation and Universities, and Make Our Planet Great Again Postdoctoral grant from Campus France
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Article Subject ,Genetics ,Animal Science and Zoology ,[SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy ,Molecular Biology ,Ecology, Evolution, Behavior and Systematics - Abstract
International audience; The taxonomy of earthworms has been riddled by instability, lack of systematically useful characters, and lax diagnoses of some genera. This has led to the use of some genera such as Allolobophora Eisen, 1874 as taxonomic wastebaskets, blurring their evolution and biogeographical history. The implementation of molecular techniques has revolutionized the systematics of the genus; however, some of its species have not been previously included in molecular phylogenetic analyses. Thus, the molecular markers COI, 16S, ND1, 12S, and 28S were sequenced for six endemic species including several taxa of Allolobophora and Aporrectodea Örely, 1885 (another related catch-all genus). Phylogenetic relationships determined by Bayesian inference and maximum likelihood analyses support the status of two of the six taxa examined (Allolobophora burgondiae Bouché, 1972 and Aporrectodea icterica Savigny, 1826) as part of Allolobophora sensu stricto and a presumed synonymy between Allolobophora and Heraclescolex Qiu and Bouché, 1998. Branch lengths and average pairwise genetic distances support the transfer of Allolobophora satchelli Bouché, 1972 to the genus Panoniona Mršić and Šapkarev, 1988 and the emergence of two new genera, Heraultia gen. nov. and Vosgesia gen. nov., endemic to France, hosting Allolobophora tiginosa Bouché, 1972 and Allolobophora zicsii Bouché, 1972, respectively. The aforementioned changes of status and the diagnosis for Heraultia and Vosgesia are presented. These results provided more evolutionarily and biogeographically coherent earthworm groups and highlighted that the Maghreb and the area around the Alps are potential key locations for the diversification of Allolobophora and several lineages of Lumbricidae.
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- 2023
20. Ovary micromorphology in hormogastrid earthworms with a particular emphasis on the organization of the germline cysts
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Piotr Świątek, Marta Novo, Daniel Fernández Marchán, Łukasz Gajda, Karol Małota, and Anna Z. Urbisz
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Animal Science and Zoology - Published
- 2023
21. The second wave of earthworm invasions in North America: biology, environmental impacts, management and control of invasive jumping worms
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Marie R. Johnston, Annise Dobson, Marie Luise Carolina Bartz, Justin B. Richardson, Mac A. Callaham, George G. Brown, Bradley M. Herrick, Jaime Ortiz-Pachar, Rebecca A. Pinder, Maryam Nouri-Aiin, Marta Novo, Erin K. Cameron, Bruce A. Snyder, Katalin Szlavecz, Tami S. Ransom, Samuel W. James, Timothy S. McCay, Andrea Dávalos, Hiroshi Ikeda, Yukio Minamiya, Josef H. Görres, Damhnait McHugh, and Chih-Han Chang
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Ecology ,biology ,Habitat ,Soil biodiversity ,Earthworm ,Forest ecology ,Plant community ,Plant litter ,biology.organism_classification ,Temperate rainforest ,Ecology, Evolution, Behavior and Systematics ,Trophic level - Abstract
The invasion of jumping worms, a small group of pheretimoid earthworm species from Asia, has increasingly become an ecological, environmental and conservation issue in forest ecosystems and urban-suburban landscapes around the world. Their presence is often noticed due to their high abundance, distinctive “jumping” behavior, and prominent granular casts on the soil surface. Although they are known to affect soil carbon dynamics and nutrient availability, no single paper has summarized their profound impacts on soil biodiversity, plant community, and animals of all trophic groups that rely on soil and the leaf litter layer for habitat, food, and shelter. In this study, we summarize the biology, invasion, and ecological impacts of invasive jumping worms across North America. We highlight potential impacts of this second wave of earthworm invasion, contrast them with the preceding European earthworm invasion in temperate forests in North America, and identify annual life cycle, reproductive and cocoon survival strategies, casting behavior and co-invasion dynamics as the key factors that contribute to their successful invasion and distinct ecological impacts. We then suggest potential management and control strategies for practitioners and policy makers, underscore the importance of coordinated community science projects in tracking the spread, and identify knowledge gaps that need to be addressed to understand and control the invasion.
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- 2021
22. Sorry atlanticus, you are not my type: molecular assessment splits Zophoscolex (Lumbricidae: Crassiclitellata) into French and Iberian genera
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Darío J. Díaz Cosín, Sergio Jiménez Pinadero, Daniel Fernández Marchán, Jorge Domínguez, Marta Novo, and Dolores Trigo
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0106 biological sciences ,0301 basic medicine ,biology ,Zoology ,010603 evolutionary biology ,01 natural sciences ,Zophoscolex ,03 medical and health sciences ,030104 developmental biology ,Type (biology) ,biology.animal ,Lumbricidae ,Animal Science and Zoology ,Ecology, Evolution, Behavior and Systematics - Abstract
Molecular phylogenetics contributes to making important advances in the challenging field of earthworm taxonomy. Use of this type of analysis has enabled clarification of the phylogenetic relationships between early-branching genera of Lumbricidae within the highly diverse Franco-Iberian realm. However, molecular phylogenetic studies of the genus Zophoscolex are scarce and have led to taxonomic uncertainty due to insufficient sampling and the absence of the type species, Z. atlanticus, from such studies. The present study investigated 11 species of Zophoscolex (including Z. atlanticus), and the phylogenetic relationships were deduced from seven molecular markers (COI, COII, 16S, tRNAs, ND1, 12S, 28S) by Bayesian and maximum likelihood inference. The findings show that species of Zophoscolex did not belong to a single clade. Zophoscolex atlanticus was placed in a clade with Z. micellus, Z. graffi and Ethnodrilus zajonci. Other species of the genus were found to belong to the genera Cataladrilus and Compostelandrilus. Finally, most of the Iberian species form a distinct clade, which was formally described as the revised genus Castellodrilus. Based on these findings, Zophoscolex is restricted to French representatives. These results highlight the importance of incorporating type species in molecular phylogenetic analyses in order to reconcile taxonomy and systematics.
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- 2021
23. Persistent pesticides: effects of endosulfan at the molecular level on the aquatic invertebrate Chironomus riparius
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Ana-Belén Muñiz-González, José Luis Martínez-Guitarte, and Marta Novo
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Health, Toxicology and Mutagenesis ,ved/biology.organism_classification_rank.species ,010501 environmental sciences ,Biology ,01 natural sciences ,Chironomidae ,Aquatic toxicology ,Persistence (computer science) ,chemistry.chemical_compound ,Animals ,Environmental Chemistry ,Ecotoxicology ,Pesticides ,Endosulfan ,0105 earth and related environmental sciences ,Chironomus riparius ,ved/biology ,General Medicine ,Pesticide ,Pollution ,Acetylcholinesterase ,Metabolic pathway ,chemistry ,Biochemistry ,Larva ,Water Pollutants, Chemical - Abstract
Although banned in multiple areas, due to its persistence in the environment, endosulfan constitutes a significant environmental concern. In this work, fourth instar Chironomus riparius larvae were exposed at environmentally relevant endosulfan concentrations of 0.1, 1, and 10 μg/L for 24 h to analyze the possible effects of this acaricide on gene expression and enzymatic activity. Transcriptional changes were studied through the implementation of a real-time polymerase chain reaction array with 42 genes related to several metabolic pathways (endocrine system, detoxification response, stress response, DNA reparation, and immune system). Moreover, glutathione-S-transferase (GST), phenoloxidase (PO), and acetylcholinesterase (AChE) activities were assessed. The five pathways were differentially altered by endosulfan exposure with significant changes in the E93, Dis, MAPR, Met, InR, GSTd3, GSTt3, MRP1, hsp70, hsp40, hsp24, ATM, PARP, Proph, and Def genes. Besides, all of the measured enzymatic activities were modified, with increased activity of GST, followed by PO and AChE. In summary, the results reflected the effects provoked in C. riparius at molecular level despite the absence of lethality. These data raise concerns about the strong alteration on different metabolic routes despite the low concentrations used. Therefore, new risk assessment strategies should consider include the effects at the sub-organismal level as endpoints in addition to the classical ecologically relevant parameters (such as survival). This endeavor will facilitate a comprehensive evaluation of toxicants in the environment.
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- 2021
24. The era of reference genomes in conservation genomics
- Author
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Giulio Formenti, Kathrin Theissinger, Carlos Fernandes, Iliana Bista, Aureliano Bombarely, Christoph Bleidorn, Claudio Ciofi, Angelica Crottini, José A. Godoy, Jacob Höglund, Joanna Malukiewicz, Alice Mouton, Rebekah A. Oomen, Sadye Paez, Per J. Palsbøll, Christophe Pampoulie, María J. Ruiz-López, Hannes Svardal, Constantina Theofanopoulou, Jan de Vries, Ann-Marie Waldvogel, Guojie Zhang, Camila J. Mazzoni, Erich D. Jarvis, Miklós Bálint, Fedor Čiampor, Jacob Hoglund, Per Palsbøll, María José Ruiz-López, Goujie Zhang, Erich Jarvis, Sargis A. Aghayan, Tyler S. Alioto, Isabel Almudi, Nadir Alvarez, Paulo C. Alves, Isabel R. Amorim, Agostinho Antunes, Paula Arribas, Petr Baldrian, Paul R. Berg, Giorgio Bertorelle, Astrid Böhne, Andrea Bonisoli-Alquati, Ljudevit L. Boštjančić, Bastien Boussau, Catherine M. Breton, Elena Buzan, Paula F. Campos, Carlos Carreras, L. FIlipe Castro, Luis J. Chueca, Elena Conti, Robert Cook-Deegan, Daniel Croll, Mónica V. Cunha, Frédéric Delsuc, Alice B. Dennis, Dimitar Dimitrov, Rui Faria, Adrien Favre, Olivier D. Fedrigo, Rosa Fernández, Gentile Francesco Ficetola, Jean-François Flot, Toni Gabaldón, Dolores R. Galea Agius, Guido R. Gallo, Alice M. Giani, M. Thomas P. Gilbert, Tine Grebenc, Katerina Guschanski, Romain Guyot, Bernhard Hausdorf, Oliver Hawlitschek, Peter D. Heintzman, Berthold Heinze, Michael Hiller, Martin Husemann, Alessio Iannucci, Iker Irisarri, Kjetill S. Jakobsen, Sissel Jentoft, Peter Klinga, Agnieszka Kloch, Claudius F. Kratochwil, Henrik Kusche, Kara K.S. Layton, Jennifer A. Leonard, Emmanuelle Lerat, Gianni Liti, Tereza Manousaki, Tomas Marques-Bonet, Pável Matos-Maraví, Michael Matschiner, Florian Maumus, Ann M. Mc Cartney, Shai Meiri, José Melo-Ferreira, Ximo Mengual, Michael T. Monaghan, Matteo Montagna, Robert W. Mysłajek, Marco T. Neiber, Violaine Nicolas, Marta Novo, Petar Ozretić, Ferran Palero, Lucian Pârvulescu, Marta Pascual, Octávio S. Paulo, Martina Pavlek, Cinta Pegueroles, Loïc Pellissier, Graziano Pesole, Craig R. Primmer, Ana Riesgo, Lukas Rüber, Diego Rubolini, Daniele Salvi, Ole Seehausen, Matthias Seidel, Simona Secomandi, Bruno Studer, Spyros Theodoridis, Marco Thines, Lara Urban, Anti Vasemägi, Adriana Vella, Noel Vella, Sonja C. Vernes, Cristiano Vernesi, David R. Vieites, Robert M. Waterhouse, Christopher W. Wheat, Gert Wörheide, Yannick Wurm, Gabrielle Zammit, Repositório da Universidade de Lisboa, Formenti, Giulio, Theissinger, Kathrin, Fernandes, Carlo, Bista, Iliana, Bombarely, Aureliano, Bleidorn, Christoph, Ciofi, Claudio, Crottini, Angelica, Godoy, José A., Höglund, Jacob, Malukiewicz, Joanna, Mouton, Alice, Oomen, Rebekah A., Paez, Sadye, Palsbøll, Per J., Pampoulie, Christophe, Ruiz-López, María J., Svardal, Hanne, Theofanopoulou, Constantina, de Vries, Jan, Waldvogel, Ann-Marie, Zhang, Guojie, Mazzoni, Camila J., Jarvis, Erich D., Bálint, Mikló, Čiampor, Fedor, Hoglund, Jacob, Palsbøll, Per, José Ruiz-López, María, Zhang, Goujie, Jarvis, Erich, Aghayan, Sargis A., Alioto, Tyler S., Almudi, Isabel, Alvarez, Nadir, Alves, Paulo C., R Amorim, Isabel, Antunes, Agostinho, Arribas, Paula, Baldrian, Petr, R Berg, Paul, Bertorelle, Giorgio, Böhne, Astrid, Bonisoli-Alquati, Andrea, L Boštjančić, Ljudevit, Boussau, Bastien, M Breton, Catherine, Buzan, Elena, F Campos, Paula, Carreras, Carlo, FIlipe Castro, L., Chueca, Luis J., Conti, Elena, Cook-Deegan, Robert, Croll, Daniel, V Cunha, Mónica, Delsuc, Frédéric, Dennis, Alice B., Dimitrov, Dimitar, Faria, Rui, Favre, Adrien, Fedrigo, Olivier D., Fernández, Rosa, Francesco Ficetola, Gentile, Flot, Jean-Françoi, Gabaldón, Toni, Galea Agius, Dolores R., Gallo, Guido R., Giani, Alice M., Thomas P Gilbert, M., Grebenc, Tine, Guschanski, Katerina, Guyot, Romain, Hausdorf, Bernhard, Hawlitschek, Oliver, D Heintzman, Peter, Heinze, Berthold, Hiller, Michael, Husemann, Martin, Iannucci, Alessio, Irisarri, Iker, S Jakobsen, Kjetill, Jentoft, Sissel, Klinga, Peter, Kloch, Agnieszka, F Kratochwil, Claudiu, Kusche, Henrik, KS Layton, Kara, A Leonard, Jennifer, Lerat, Emmanuelle, Liti, Gianni, Manousaki, Tereza, Marques-Bonet, Toma, Matos-Maraví, Pável, Matschiner, Michael, Maumus, Florian, Mc Cartney, Ann M., Meiri, Shai, Melo-Ferreira, José, Mengual, Ximo, Monaghan, Michael T., Montagna, Matteo, Robertwmysłajek, T Neiber, Marco, Nicolas, Violaine, Novo, Marta, Ozretić, Petar, Palero, Ferran, Pârvulescu, Lucian, Pascual, Marta, Paulo, Octávio S., Pavlek, Martina, Pegueroles, Cinta, Pellissier, Loc, Pesole, Graziano, R Primmer, Craig, Riesgo, Ana, Rüber, Luka, Rubolini, Diego, Salvi, Daniele, Seehausen, Ole, Seidel, Matthia, Secomandi, Simona, Studer, Bruno, Theodoridis, Spyro, Thines, Marco, Urban, Lara, Vasemägi, Anti, Vella, Adriana, Vella, Noel, C Vernes, Sonja, Vernesi, Cristiano, R Vieites, David, M Waterhouse, Robert, W Wheat, Christopher, Wörheide, Gert, Wurm, Yannick, Zammit., and Gabrielle, Bioinformatique, phylogénie et génomique évolutive (BPGE), Département PEGASE [LBBE] (PEGASE), Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Eléments transposables, évolution, populations, Département génétique, interactions et évolution des génomes [LBBE] (GINSENG), Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), Barcelona Supercomputing Center, European Reference Genome Atlas (ERGA) Consortium, University of St Andrews. School of Biology, University of St Andrews. St Andrews Bioinformatics Unit, Palsbøll lab, Marine Biology, University of Zurich, and Bálint, Miklós
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QH301 Biology ,580 Plants (Botany) ,Genetics -- Research ,Evolutionsbiologi ,biodiversity conservation ,conservation genetics ,ERGA ,European Reference Genome Atlas ,Conservation genetics ,Biodiversity conservation ,Animal genome mapping ,udc:630*1 ,Genome ,GE ,[SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] ,[SDE.BE.BIOD]Environmental Sciences/Biodiversity and Ecology/domain_sde.be.biod ,Biodiversity [MeSH] ,Genomics [MeSH] ,Ecology, Evolution, Behavior and Systematics ,Genome [MeSH] ,3rd-DAS ,Genomics ,Biodiversity ,referenčni genomi ,[SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM] ,[SDE.BE.BEC]Environmental Sciences/Biodiversity and Ecology/domain_sde.be.bec ,Chemistry ,10121 Department of Systematic and Evolutionary Botany ,genomika ,GE Environmental Sciences ,Informàtica::Aplicacions de la informàtica::Bioinformàtica [Àrees temàtiques de la UPC] ,biodiverziteta ,Settore BIO/18 - GENETICA ,education ,QH426 Genetics ,QH301 ,VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470 ,[SDE.BE.EVO]Environmental Sciences/Biodiversity and Ecology/domain_sde.be.evo ,Genetics ,genomi ,10211 Zurich-Basel Plant Science Center ,Genomes ,Genetik ,Biology ,QH426 ,Evolutionary Biology ,Ambientale ,Ecología ,Genética ,1105 Ecology, Evolution, Behavior and Systematics ,Wildlife conservation ,570 Life sciences ,biology ,Human medicine ,Animal genetics ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology ,Genètica - Abstract
Progress in genome sequencing now enables the large-scale generation of reference genomes. Various international initiatives aim to generate reference genomes representing global biodiversity. These genomes provide unique insights into genomic diversity and architecture, thereby enabling comprehensive analyses of population and functional genomics, and are expected to revolutionize conservation genomics., Trends in Ecology & Evolution, 37 (3), ISSN:0169-5347, ISSN:1872-8383
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- 2022
25. Toxicogenomics in a soil sentinel exposure to Zn nanoparticles and ions reveals the comparative role of toxicokinetic and toxicodynamic mechanisms
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Marta Novo, Maria Diez Ortiz, David J. Spurgeon, Peter Kille, and Elma Lahive
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0303 health sciences ,Eisenia fetida ,Toxicodynamics ,biology ,Chemistry ,Materials Science (miscellaneous) ,Metal ions in aqueous solution ,010501 environmental sciences ,biology.organism_classification ,01 natural sciences ,03 medical and health sciences ,Cytosol ,Toxicity ,Biophysics ,Toxicokinetics ,Toxicogenomics ,030304 developmental biology ,0105 earth and related environmental sciences ,General Environmental Science ,EC50 - Abstract
A critical question for read across of the hazards of nanomaterials is the degree to which their mechanisms of action differ from those of their bulk chemical constituents. It has been established that metal and metal oxide nanoparticles (NPs) can be accumulated by invertebrate species. Moreover, it has been hypothesised that the observed toxicity resulting on exposure to these nanomaterials is most likely to be associated with the releases of ions from external or internal dissolution leading ultimately to toxicity. However, mechanistic confirmation of the similar modes of action for metal oxide nanomaterials and metal ions in studies invertebrates are largely lacking. Therefore, here we present a toxicogenomic study using exposed individuals of the earthworm Eisenia fetida from a single genetic lineage. We compared gene expression and pathway responses through RNA-seq analysis at equitoxic concentrations (EC50 for reproduction) of ZnO NPs and ionic Zn. We found similar transcriptomic effects for both Zn forms with genetic signatures of tight control of cytosolic Zn concentrations through expression changes of genes encoding several Zn transporters. Activation and regulation of JUN, MAP and JNK kinases indicated a cellular response to the increased Zn concentrations of both forms with compound binding terms also enriched. Our results illustrate the need to consider both toxicokinetic and toxicodynamic mechanisms in the development of adverse outcome pathways for nanomaterials.
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- 2020
26. Guess who? Taxonomic problems in the genus Eiseniella revisited by integrated approach
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Irene de Sosa, Daniel F. Marchán, Marta Novo, Timea Szederjesi, Misel Jelic, Aleksandra Jabłońska, Raúl Navarro, Ana Almodóvar, and Darío J. Díaz Cosín
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Invertebrados ,Ecology, Evolution, Behavior and Systematics ,Earthworm ,Genetic diversity ,Morphology ,Phylogeny ,Semi-aquatic habitat - Abstract
Eiseniella neapolitana is a semi-aquatic, diploid earthworm that for many years was related to the cosmopolitan species Eiseniella tetraedra and even considered a subspecies of it. Norealidys andaluciana was described in Spain and is usually synonymized with E. neapolitana. We collected 69 specimens from Italy, Spain, and Cyprus and studied five molecular markers (COI, 16S, 28S, 12S, and ND1) and their morphology to solve this taxonomic problem. Phylogenetic analyses reveal the possible existence of two separate genera confounded under the name Eiseniella, but the study of more molecular markers and species of the genus would be necessary to confirm this. Therefore, the synonymy between Eiseniella and Norealidys is maintained. Various genetic analyses, including species delimitation, confirm the separation between E. neapolitana and E. andaluciana (= N. andaluciana) and excluded that E. neapolitana is a subspecies of E. tetraedra. The resemblance in external appearance despite clear genetic differences of the three species could be explained by convergent adaptation to the aquatic habitat. Despite the expected low haplotype diversity based on the 28S gene, we found a surprisingly high variability in the E. andaluciana (= N. andaluciana) population in Spain. However, its stable predicted secondary structure and its high content of G + C reject the presence of a pseudogene.
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- 2022
27. NEONATAL GROUP B STREPTOCOCCAL DISEASE IN A NEONATAL INTENSIVE CARE UNIT: A TWELVE-YEAR STUDY
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Marta Novo, Dinis ada, Marta Soares, Ana Ramalho, Maria Alfaro, Ecaterina Scortenschi, Cla Calado, Jo Rosa, Vera Santos, and Luisa Gaspar
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General Medicine - Abstract
Introduction: Group B Streptococcus (GBS) is a gram-positive coccus, that frequently colonizes the human genital and gastrointestinal tract, and is the most common cause of invasive infections in neonates. The high clinical suspicion and early treatment are essential to prevent devastating sequelae and improve the prognosis of these patients. Materials and Methods: A retrospective descriptive study made with children younger than 3 months of age, admitted to a neonatal intensive care unit of a level II hospital, from January 1, 2010 to December 31, 2021, with early (EOI) or late-onset (LOI) neonatal GBS infection. Reviewed obstetric history, risk factors, intrapartum chemoprophylaxis (IPA), clinical manifestations, antibiotic therapy, supportive care, recurrent infection, morbidities and deaths. Results: There were 6 cases registered during 12-year period with GBS infection, 3(50%) had EOI, 3(50%) had LOI and 1 case with recurrent infection (more 2 episodes). Most cases were prematures (4/6; 67%)with average gestational age of 29weeks. Eight GBS infections were documented. In EOI, prematurity and prolonged rupture of membranes without chemoprophylaxis were identified as risk factors. The most common clinical diagnosis was meningitis (62,5%, 5/8) and sepsis/bacteriemia without a focus (37,5%, 3/8). The most used empirical therapy was ampicillin, gentamicin and cefotaxime (50%, 4/8) and the most frequent definitive therapy was Penicillin G. 4 patients needed ventilatory support and 1 needed shock and seizures treatment. In one case hydrocephalus and delayed psychomotor development (DPD) were reported and in another case DPD and eyelid ptosis were also reported. 1 death occurred. Conclusion: In our study, maternal colonization wasn’t a risk factor for EIO, because the 4 mothers who performed GBS culture (2 mothers of term newborns and 2 mothers of preterms, one with invasive disease and other with recurrent infection) were negative. We obtained a higher percentage of meningitis than previously described (62.5% vs 5-10%), probably because most patients were premature and immunosuppressed.
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- 2023
28. The Bat Child
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Marta Novo, Maria Gaia, Helena Santos, and Mafalda Santos
- Subjects
General Medicine - Abstract
Case Report A short stature 10 year old boy with pes planus, mild hearing loss, failure of exfoliation of the primary dentition, anterior hyperdontia, malocclusion and open anterior fontanelle was observed in Pediatric Orthopedics consultation, and presenting the following alterations (image). Discussion This child has cleidocranial dysplasia (CCD). Is an autosomal dominant disease caused by an heterozygous loss-of-function mutation in the RUNX2 gene, encoding transcription factor CBFA1, on chromosome 6p21, which plays an important role in osteogenesis and differentiation of osteoblasts. The main clinical features include persistently open skull sutures, hypoplasia or aplasia of the clavicles permitting abnormal facility in opposing the shoulders, short middle phalanx of the fifth fingers, dental anomalies (supernumerary teeth, failure of exfoliation of the primary dentition and malocclusion), pes planus, upper respiratory complications, recurrent otitis media, hearing loss and often vertebral malformation (scoliosis). Conclusion The clinical diagnosis of CCD was made during pregnancy, through the detection of aplasia of the clavicles in fetu, in association with the mother having short stature (1.32 meters), persistence of primary teeth and open anterior fontanelle. With 10 years of age, he underwent mendilioma study which revealed the mutation in the RUNX2 gene (c. 747 del (p.(Arg251 Alafs*7))) classified as a pathogenic variant.
- Published
- 2023
29. Transcriptome analysis of aphids exposed to glandular trichomes in tomato reveals stress and starvation related responses
- Author
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Rosario Planelló, Lola Llorente, Óscar Herrero, Marta Novo, Lidia Blanco-Sánchez, Juan Antonio Díaz-Pendón, Rafael Fernández-Muñoz, Victoria Ferrero, and Eduardo de la Peña
- Subjects
Multidisciplinary ,BEMISIA-TABACI ,Gene Expression Profiling ,PEA APHID ,Biology and Life Sciences ,WILD ,Trichomes ,METABOLISM ,HOST-PLANT ,PLANT VOLATILES ,2 STRAINS ,Solanum lycopersicum ,WINGED FORMS ,Aphids ,Animals ,Herbivory ,RESISTANCE ,DETOXIFICATION - Abstract
Understanding the responses of insect herbivores to plant chemical defences is pivotal for the management of crops and pests. However, the mechanisms of interaction are not entirely understood. In this study, we compared the whole transcriptome gene expression of the aphid Macrosiphum euphorbiae grown on two different varieties of tomato that differ in their inducible chemical defences. We used two isogenic lines of tomato with a shared genetic background that only differ in the presence of type IV glandular trichomes and their associated acylsucrose excretions. This works also reports a de novo transcriptome of the aphid M. euphorbiae. Subsequently, we identified a unique and distinct gene expression profile for the first time corresponding to aphid´s exposure to type IV glandular trichomes and acylsugars. The analysis of the aphid transcriptome shows that tomato glandular trichomes and their associated secretions are highly efficient in triggering stress-related responses in the aphid, and demonstrating that their role in plant defence goes beyond the physical impediment of herbivore activity. Some of the differentially expressed genes were associated with carbohydrate, lipid and xenobiotic metabolisms, immune system, oxidative stress response and hormone biosynthesis pathways. Also, the observed responses are compatible with a starvation syndrome. The transcriptome analysis puts forward a wide range of genes involved in the synthesis and regulation of detoxification enzymes that reveal important underlying mechanisms in the interaction of the aphid with its host plant and provides a valuable genomic resource for future study of biological processes at the molecular level using this aphid.
- Published
- 2021
30. Understanding Diversity, Evolution, and Structure of Small Heat Shock Proteins in Annelida Through in Silico Analyses
- Author
-
Mercedes de la Fuente and Marta Novo
- Subjects
Physiology ,Physiology (medical) - Abstract
Small heat shock proteins (sHsps) are oligomeric stress proteins characterized by an α-crystallin domain (ACD). These proteins are localized in different subcellular compartments and play critical roles in the stress physiology of tissues, organs, and whole multicellular eukaryotes. They are ubiquitous proteins found in all living organisms, from bacteria to mammals, but they have never been studied in annelids. Here, a data set of 23 species spanning the annelid tree of life, including mostly transcriptomes but also two genomes, was interrogated and 228 novel putative sHsps were identified and manually curated. The analysis revealed very high protein diversity and showed that a significant number of sHsps have a particular dimeric architecture consisting of two tandemly repeated ACDs. The phylogenetic analysis distinguished three main clusters, two of them containing both monomeric sHsps, and ACDs located downstream in the dimeric sHsps, and the other one comprising the upstream ACDs from those dimeric forms. Our results support an evolutionary history of these proteins based on duplication events prior to the Spiralia split. Monomeric sHsps 76) were further divided into five subclusters. Physicochemical properties, subcellular location predictions, and sequence conservation analyses provided insights into the differentiating elements of these putative functional groups. Strikingly, three of those subclusters included sHsps with features typical of metazoans, while the other two presented characteristics resembling non-metazoan proteins. This study provides a solid background for further research on the diversity, evolution, and function in the family of the sHsps. The characterized annelid sHsps are disclosed as essential for improving our understanding of this important family of proteins and their pleotropic functions. The features and the great diversity of annelid sHsps position them as potential powerful molecular biomarkers of environmental stress for acting as prognostic tool in a diverse range of environments.
- Published
- 2021
31. Ciencia política contemporánea
- Author
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Diz Otero, Isabel, Lois González, Marta, Novo Vázquez, Amparo and Diz Otero, Isabel, Lois González, Marta, Novo Vázquez, Amparo
- Published
- 2012
32. How to resist soil desiccation: Transcriptional changes in a Mediterranean earthworm during aestivation
- Author
-
Marta Novo and Natasha Tilikj
- Subjects
Transcription, Genetic ,Physiology ,Invertebrados ,Down-Regulation ,Biochemistry ,Transcriptome ,Soil ,Stress, Physiological ,Animals ,KEGG ,Desiccation ,Oligochaeta ,Molecular Biology ,Ecosystem ,biology ,Abiotic stress ,Ecology ,Mediterranean Region ,Gene Expression Profiling ,Earthworm ,biology.organism_classification ,Droughts ,Estivation ,Gene Ontology ,Spain ,Aestivation ,Dormancy ,Adaptation ,Desert Climate - Abstract
Earthworms have a central role in ministering the terrestrial ecosystems and are proving to have an important role in modulating the effects climate change has on soil. Aestivation is a form of dormancy employed by the organisms living in deserts and arid environments, when confronted with prolonged periods of drought. Understanding global metabolic adjustments required for withstanding the harsh conditions of the ever more severe Iberian drought, we performed a global transcriptomic exploration of the endogeic earthworm Carpetania matritensis during aestivation. There were a total of 6352 differentially expressed transcripts in the aestivating group, with 65% being downregulated. Based on GO and KEGG enrichment analyses, downregulated genes seem to be indicative of an overall metabolic depression during aestivation. Indeed we noted a reduction of protein turnover and macromolecule metabolism coupled with suppression of genes involved in digestion. Upregulated genes, namely antioxidant genes and DNA repair genes showed clear signs of abiotic stress caused by ROS generation. Abiotic stress led to transcriptomic changes of genes involved in immune response, mostly affecting the NF-kb signaling pathway as well as changes in apoptotic genes indicating the necessity of investigating these processes in a tissue specific manner. Lastly we uncovered a possible mechanism for water retention by nitrogenous waste accumulation. This study provides the first ever transcriptomic investigation done on aestivating earthworms and as such serves as a general framework for investigation on other earthworm species and other soil invertebrates, which is becoming increasingly important with the current scenario of climate change.
- Published
- 2021
33. The Aqīt Household: Professional Mobility of a Berber Learned Elite in Premodern West Africa
- Author
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Marta Novo
- Published
- 2021
34. Correction to: A strong backbone for an invertebrate group: anchored phylogenomics improves the resolution of genus-level relationships within the Lumbricidae (Annelida, Crassiclitellata)
- Author
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Daniel F. Marchán, Samuel W. James, Alan R. Lemmon, Emily Moriarty Lemmon, Marta Novo, Jorge Domínguez, Darío J. Díaz Cosín, and Dolores Trigo
- Subjects
Ecology, Evolution, Behavior and Systematics - Published
- 2022
35. Applying sunscreens on earthworms: Molecular response of Eisenia fetida after direct contact with an organic UV filter
- Author
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Dolores Trigo, Ana-Belén Muñiz-González, Marta Novo, S. Casquero, and J.L. Martínez Guitarte
- Subjects
Receptors, Steroid ,Eisenia fetida ,Environmental Engineering ,010504 meteorology & atmospheric sciences ,UV filter ,Endocrine System ,Endocrine Disruptors ,010501 environmental sciences ,medicine.disease_cause ,01 natural sciences ,Superoxide dismutase ,chemistry.chemical_compound ,Gene expression ,medicine ,Animals ,Environmental Chemistry ,Oligochaeta ,Waste Management and Disposal ,0105 earth and related environmental sciences ,biology ,Superoxide Dismutase ,biology.organism_classification ,Pollution ,Endocrine disruptor ,chemistry ,Biochemistry ,biology.protein ,Xenobiotic ,Ecdysone receptor ,Sunscreening Agents ,Genotoxicity - Abstract
The use of organic Ultraviolet (UV) filters has increased in the last years, either in sunscreens, other cosmetics, or even food packaging. These filters may end up in soil and water since the Wastewater Treatment Plants may not successfully remove them. Among them, benzophenones are known to act as endocrine disruptors. However, most of the studies are directed towards vertebrates and aquatic invertebrates, while there is a lack of information on the molecular mechanisms affected by these compounds on soil dwelling invertebrates. Here, we study the impact of direct acute (48 h) contact of 4-hydroxybenzophenone (4-OHBP) at two sublethal concentrations (0.02 and 0.2 mg/mL) on gene expression of the earthworm Eisenia fetida. Investigated genes were involved in endocrine pathways, stress response, detoxification mechanisms, genotoxicity, energy metabolism and epigenetics. Three of them were identified for the first time in earthworms. Our results suggest that exposure to 4-OHBP affected endocrine pathways, causing an increase in the Ecdysone receptor gene (EcR) expression. Moreover, the UV filter induced changes in the CuZn superoxide dismutase gene (CuZn SOD), indicating an effect in the stress response. Finally, significant changes were detected for glyceraldehyde-3-phosphate dehydrogenase gene (GAPDH) expression, indicating that energy metabolism is influenced by the 4-OHBP and highlighting the risks of using GAPDH as an internal reference for Real Time PCR.
- Published
- 2019
36. The lab In A box: A take‐out practical experience for an online invertebrate biology course
- Author
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Rosario G. Cánovas, Darío J. Díaz Cosín, Dolores Trigo, Mónica Gutiérrez López, Nuria Sánchez, Fernando Pardos, and Marta Novo
- Subjects
Active learning ,Online teaching ,Mathematics education ,Invertebrate Biology ,Animal Science and Zoology ,Biology ,Course (navigation) - Published
- 2021
37. Neonatal clinical case
- Author
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Ana Cristina Fernandes, Raquel Lima, Marta Novo, Francisco Medina, and Andreia Pereira
- Subjects
newborn ,subcutaneous fat necrosis ,RG1-991 ,tumefaction ,hypercalcemia ,Gynecology and obstetrics ,Pediatrics ,RJ1-570 - Abstract
Submitted by Ana Pinto (u13776@chporto.min-saude.pt) on 2021-11-23T11:44:00Z No. of bitstreams: 1 neonatalcli.pdf: 947059 bytes, checksum: bc7b62b39cb125b779a10db7490ac117 (MD5) Made available in DSpace on 2021-11-23T11:44:00Z (GMT). No. of bitstreams: 1 neonatalcli.pdf: 947059 bytes, checksum: bc7b62b39cb125b779a10db7490ac117 (MD5) Previous issue date: 2020-12 info:eu-repo/semantics/publishedVersion
- Published
- 2020
38. How to thrive in unstable environments: Gene expression profile of a riparian earthworm under abiotic stress
- Author
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Irene de Sosa, Aída Verdes, Natasha Tilikj, Daniel F. Marchán, Rosario Planelló, Óscar Herrero, Ana Almodóvar, Darío Díaz Cosín, Marta Novo, Universidad Complutense de Madrid, European Commission, and Ministerio de Ciencia e Innovación (España)
- Subjects
Environmental Engineering ,Invertebrados ,Eiseniella tetraedra ,Pollution ,Cold Temperature ,Stress, Physiological ,Freezing ,Climate change ,Animals ,Environmental Chemistry ,Desiccation ,Oligochaeta ,Transcriptomics ,Transcriptome ,Waste Management and Disposal ,Cold - Abstract
Nowadays, extreme weather events caused by climate change are becoming more frequent. This leads to the occurrence of extreme habitats to which species must adapt. This challenge becomes crucial for species living in unstable environments, such as the riparian earthworm Eiseniella tetraedra. Its cosmopolitan distribution exposes it to various environmental changes, such as freezing in subarctic regions or droughts in Mediterranean areas. Transcriptional changes under cold and desiccation conditions could therefore shed light on the adaptive mechanisms of this species. An experiment was performed for each condition. In the cold experiment, the temperature was lowered to −14 °C ± 2 °C (compared to 8 °C for control samples), and in the desiccation treatment, humidity was lowered from 60% to 15%. Comparisons of gene expression levels between earthworms under freezing conditions and control earthworms revealed a total of 84 differentially expressed genes and comparisons between the desiccation experiment and the control yielded 163 differentially expressed genes. However, no common responses were found between the two treatments. The results suggest that E. tetraedra can acclimate to low temperatures due to the upregulation of genes involved in glucose accumulation. However, downregulation of the respiratory chain suggests that this earthworm does not tolerate freezing conditions. Under desiccation conditions, genes involved in cell protection from apoptosis and DNA repair were upregulated. In contrast, lipid metabolism was downregulated, presumably to conserve resources by reducing the rate at which they are consumed., IS was supported by a Predoctoral Fellowship grant by Universidad Complutense de Madrid, Spain. AV was funded by the European Union's Horizon 2020 research and innovation program through a Marie Sklodowska-Curie individual fellowship (841576). MN was supported by Ramón y Cajal Fellowship (RYC2018-024654-I) and this study was funded by Grant PGC2018-094112-A-I00, both from MCIN/AEI/10.13039/501100011033 and by “ESF: Investing in your future” and “ERDF: A way of making Europe” respectively.
- Published
- 2022
39. Genome-informed integrative taxonomic description of three cryptic species in the earthworm genus Carpetania (Oligochaeta, Hormogastridae)
- Author
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Marta Novo, Rosa Fernández, Daniel Fernández Marchán, Jorge Domínguez, Darío J. Díaz Cosín, Universidad Complutense de Madrid, Banco Santander, and Xunta de Galicia
- Subjects
0301 basic medicine ,Genotyping by sequencing ,0106 biological sciences ,Species complex ,Soil biology ,Invertebrados ,Single-nucleotide polymorphism ,Plant Science ,Biology ,010603 evolutionary biology ,01 natural sciences ,Genome ,Soil fauna ,Oligochaeta (plant) ,03 medical and health sciences ,Genus ,SNP ,Earthworms ,Clade ,Ecology, Evolution, Behavior and Systematics ,030304 developmental biology ,0303 health sciences ,Earthworm ,15. Life on land ,biology.organism_classification ,Hormogastridae ,030104 developmental biology ,Taxon ,Evolutionary biology ,Cryptic species ,Genotyping By Sequencing ,Integrative taxonomy ,Taxonomy (biology) - Abstract
Research on cryptic species complexes has reached a consensus on the necessity of integrating multiple sources of evidence. Reduced representation library techniques such as Genotyping-by-Sequencing (GBS) have proven useful to study these groups. Both integrative taxonomy and genome-wide single nucleotide polymorphism (SNP) data remain to be widely applied to earthworms, an animal group with widespread presence of cryptic diversity. The genus Carpetania (formerly the Hormogaster elisae species complex) was found to contain six deeply divergent genetic lineages and some inconspicuous morphological differentiation based on a handful of Sanger-sequenced markers. Marchán et al. (submitted) delimited three well-supported species-level clades on the basis of a genome-wide SNP dataset and geometric morphometric analyses, highlighting the necessity of a formal taxonomic description of these taxa. In this work, further analyses are applied to the SNP data and a thorough morphological study is performed in order to provide an integrative description of two new species and to redescribe Carpetania elisae. Species-specific SNPs are identified and used as diagnostic characters, and genome-wide and cytochrome oxidase C subunit 1 (COI) genetic distances are compared finding a strong correlation between them. The taxonomic description of these three cryptic species provides a useful tool to include them effectively in ecological studies and biodiversity conservation actions., This work was supported by Universidad Complutense de Madrid and Santander Group under grant Proyecto de Investigación Santander/Complutense PR41/17-21027; Systematics Research Fund (SRF) and Xunta de Galicia. Consellería de Cultura, Educación e Ordenación Universitaria. Secretaria Xeral de Universidades under grant ED431B 2019/038.
- Published
- 2020
40. Oleuropein, a component of extra virgin olive oil, lowers postprandial glycaemia in healthy subjects
- Author
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Valentina Castellani, Romano Silvestri, Cristina Nocella, Simona Bartimoccia, Vittoria Cammisotto, Marta Novo, Francesco Violi, Lorenzo Loffredo, and Roberto Carnevale
- Subjects
0301 basic medicine ,Pharmacology ,medicine.medical_specialty ,Meal ,030109 nutrition & dietetics ,Isoprostane ,business.industry ,Insulin ,medicine.medical_treatment ,030204 cardiovascular system & hematology ,medicine.disease_cause ,Placebo ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Postprandial ,Endocrinology ,chemistry ,Oleuropein ,Internal medicine ,Medicine ,Hydroxytyrosol ,Pharmacology (medical) ,business ,Oxidative stress - Abstract
AIMS Extra virgin olive oil lowers postprandial glycaemia. We investigated if oleuropein, a component of extra virgin olive oil, exerts a similar effect on postprandial glycaemia and the underlying mechanism. METHODS Twenty healthy subjects were randomly allocated in a cross-over design to 20 mg oleuropein or placebo immediately before lunch. Postprandial glycaemia along with blood insulin, dipeptidyl-peptidase-4 (DPP-4) and glucagon-like peptide-1 and oxidative stress, which included soluble NADPH oxidase-derived peptide activity (sNox2-dp), 8-iso-prostaglandin-2α and platelet p47phox phosphorylation, were analysed before and 2 h after meal. RESULTS After 2 h, subjects who assumed oleuropein had significantly lower blood glucose, DPP-4 activity and higher insulin and glucagon-like peptide-1 compared to placebo. Furthermore, sNox2-dp, 8-iso-PGF2α and platelet p47phox phosphorylation were significantly lower in oleuropein- compared to placebo-treated subjects. DPP-4 significantly correlated with sNox2-dp [Spearman's rho (Rs) = 0.615; P
- Published
- 2018
41. Endocrine disruptors in soil: Effects of bisphenol A on gene expression of the earthworm Eisenia fetida
- Author
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I. Verdú, José-Luis Martínez-Guitarte, Marta Novo, and Dolores Trigo
- Subjects
0301 basic medicine ,Eisenia fetida ,Health, Toxicology and Mutagenesis ,Gene Expression ,Physiology ,Endocrine Disruptors ,010501 environmental sciences ,Biology ,Ecotoxicology ,medicine.disease_cause ,01 natural sciences ,Soil ,03 medical and health sciences ,chemistry.chemical_compound ,Phenols ,Detoxification ,Gene expression ,medicine ,Animals ,Soil Pollutants ,Metallothionein ,Endocrine system ,Epigenetics ,Benzhydryl Compounds ,Oligochaeta ,0105 earth and related environmental sciences ,Public Health, Environmental and Occupational Health ,General Medicine ,biology.organism_classification ,Pollution ,030104 developmental biology ,chemistry ,Xenobiotic ,Biomarkers ,Genotoxicity - Abstract
Xenobiotics such as bisphenol A (BPA), are present in biosolids, which are applied as organic fertilizers in agricultural fields. Their effects on soil life have been poorly assessed, and this is particularly important in the case of earthworms, which represent the main animal biomass in this medium. In the present work we study the impacts of BPA on gene expression of Eisenia fetida, a widely used ecotoxicological model. Chronic soil tests and acute contact tests were performed, and gene expression was analyzed in total tissue and in masculine reproductive organs of the earthworms. The genes studied in this research played a role in endocrine pathways, detoxification mechanisms, stress response, epigenetics, and genotoxicity. Most of the genes were identified for the first time, providing potentially useful biomarkers for future assessments. For chronic exposures, no changes were detected in whole-body tissue; however, masculine reproductive organs showed changes in the expression of genes related to endocrine function (EcR, MAPR, AdipoR), epigenetic mechanisms (DNMTs), genotoxicity (PARP1), and stress responses (HSC70 4). For acute exposures, the expression of one epigenetic-related gene was altered for both whole-body tissues and male reproductive organs (Piwi2). Further changes were detected for whole-body tissues involved in detoxification (Metallothionein), stress (HSC70 4), and genotoxicity (PARP1) mechanisms. Acute exposure effects were also tested in whole-body tissues of juveniles, showing changes in the expression of Metallothionein and Piwi2. The molecular changes found in the analyzed earthworms indicate that exposure to BPA may have negative implications in their populations. Particularly interesting are the alterations related to epigenetic mechanisms, which suggest that future generations may be impacted. This study is the first to evaluate the molecular effects of BPA on soil organisms, and further assays will be necessary to better characterize the true environmental repercussions. Capsule: Levels of gene expression in total-body tissues and masculine reproductive organs were analyzed in earthworms after exposure to bisphenol A and we observed associated changes in detoxification, endocrine, epigenetic, genotoxic and stress pathways.
- Published
- 2018
42. Effects of dark chocolate on endothelial function in patients with non-alcoholic steatohepatitis
- Author
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Lorenzo Loffredo, Simona Battaglia, Francesco Violi, Gaetano Pannitteri, M. Del Ben, Marta Novo, Francesco Baratta, Cristina Nocella, P. Ludovica, Roberto Carnevale, Fabrizio Ceci, and Francesco Angelico
- Subjects
Male ,Time Factors ,Brachial Artery ,Endocrinology, Diabetes and Metabolism ,Rome ,Medicine (miscellaneous) ,030204 cardiovascular system & hematology ,Dinoprost ,medicine.disease_cause ,0302 clinical medicine ,Non-alcoholic Fatty Liver Disease ,Chocolate ,Endothelial dysfunction ,education.field_of_study ,Cross-Over Studies ,Nutrition and Dietetics ,Fatty liver ,food and beverages ,Middle Aged ,Vasodilation ,Treatment Outcome ,Biochemistry ,NADPH Oxidase 2 ,cardiovascular system ,Female ,030211 gastroenterology & hepatology ,Cardiology and Cardiovascular Medicine ,Adult ,medicine.medical_specialty ,Population ,Dark chocolate ,Nitric Oxide ,03 medical and health sciences ,food ,Internal medicine ,medicine ,Humans ,education ,business.industry ,medicine.disease ,food.food ,Oxidative Stress ,Milk Chocolate ,Cross-Sectional Studies ,Endocrinology ,cocoa ,endothelial function ,nicotinamide adenine dinucleotide phosphate oxidase ,non-alcoholic steatohepatitis ,oxidative stress ,polyphenols ,medicine (miscellaneous) ,endocrinology, diabetes and metabolism ,nutrition and dietetics ,cardiology and cardiovascular medicine ,Endothelium, Vascular ,Steatosis ,Steatohepatitis ,business ,Biomarkers ,Oxidative stress - Abstract
Oxidative stress plays a pivotal role in inducing endothelial dysfunction and progression from simple fatty liver steatosis (FLD) to non-alcoholic steatohepatitis (NASH). Polyphenols could reduce oxidative stress and restore endothelial function by inhibiting the nicotinamide-adenine-dinucleotide-phosphate (NADPH) oxidase isoform Nox2. The aim of this study was to assess endothelial function and oxidative stress in a population affected by simple FLD and NASH. Furthermore, we analysed the effect of high vs low content of cocoa polyphenols on endothelial function and oxidative stress in patients with NASH.In a cross-sectional study we analysed endothelial function, as assessed by flow-mediated dilation (FMD), and oxidative stress, as assessed by Nox2 activation, serum isoprostanes and nitric oxide bioavailability (NOx), in patients with NASH (n = 19), FLD (n = 19) and controls (n = 19). Then, we performed a randomized, cross-over study in 19 subjects with NASH comparing the effect of 14-days administration of 40 g of chocolate at high (dark chocolate, cocoa85%) versus low content (milk chocolate, cocoa35%) of polyphenols on FMD and oxidative stress. Compared to controls, NASH and FLD patients had higher Nox2 activity and isoprostanes levels and lower FMD and NOx, with a significant gradient between FLD and NASH. The interventional study showed that, compared to baseline, FMD and NOx increased (from 2.9 ± 2.4 to 7.2 ± 3.0% p 0.001 and from 15.9 ± 3.6 to 20.6 ± 4.9 μM, p 0.001, respectively) in subjects given dark but not in those given milk chocolate. A simple linear regression analysis showed that Δ (expressed by difference of values between before and after 14 days of chocolate assumption) of FMD was associated with Δ of Nox2 activity (Rs = -0.323; p = 0.04), serum isoprostanes (Rs: -0.553; p 0.001) and NOx (Rs: 0.557; p 0.001).Cocoa polyphenols improve endothelial function via Nox2 down-regulation in NASH patients.
- Published
- 2018
43. Effects of bisphenol S on the life cycle of earthworms and its assessment in the context of climate change
- Author
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Dolores Trigo, A. Marcos, José Luis Martínez-Guitarte, Marta Novo, Ana-Belén Muñiz-González, and N. Tilikj
- Subjects
Eisenia fetida ,Environmental Engineering ,010504 meteorology & atmospheric sciences ,biology ,Biosolids ,Earthworm ,Context (language use) ,010501 environmental sciences ,biology.organism_classification ,01 natural sciences ,Pollution ,Toxicology ,chemistry.chemical_compound ,Bisphenol S ,chemistry ,Toxicity ,Environmental Chemistry ,Xenobiotic ,Waste Management and Disposal ,hormones, hormone substitutes, and hormone antagonists ,0105 earth and related environmental sciences ,Toxicant - Abstract
Two of the main challenges facing soil organisms nowadays are emerging contaminants and climate change. The biosolids used in agriculture can contain xenobiotics, such as bisphenols, not regulated in soil. The proven harmful effect of bisphenol A (BPA) has urged the search for replacements such as bisphenol S (BPS) but no studies have tested the effects of this substitute on soil organisms. Our aim was to study the impacts of BPS on the biological cycle of two earthworm species, Dendrobaena veneta and Eisenia fetida, and compare to earlier results with BPA to confirm its suitability as replacement. Moreover, interactions with temperature were investigated, the increase of which is expected due to climate change. Sublethal concentrations (10, 100, 1000 mg kg−1) were evaluated by an artificial soil test at 21 °C. Additional tests with D. veneta were performed at 26 °C, to observe whether a 5 °C increase affected the toxicity of BPS in earthworms. In E. fetida, reproduction (number of juveniles and cocoons) was negatively affected by BPS but growth was not negatively impacted. In D. veneta reproduction was not affected and greater growth was detected in the lowest concentration tested only at 26 °C. Increase in temperature showed no interaction on BPS toxicity but had a greater negative impact than the toxicant and adults showed significantly lower growth and reproductive rate at 26 °C. A direct contact test with BPS was performed to evaluate mortality and enzymatic activity of glutathione-S-transferase (GST). The measured enzymatic activity was higher with increasing concentration of BPS at 48 h and 21 °C in D. veneta but not in E. fetida or at 26 °C. Our results suggest that BPS is slightly less toxic than BPA supporting its use as alternative and also highlight the harmful effects that an increase in temperature can have in earthworm populations, exceeding those of certain toxicants.
- Published
- 2021
44. Low-grade endotoxemia, gut permeability and platelet activation in patients with impaired fasting glucose
- Author
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Simona Bartimoccia, Marta Novo, Francesco Baratta, Cristina Nocella, M. Del Ben, Roberto Carnevale, Vittoria Cammisotto, Daniele Pastori, Francesco Angelico, Sebastiano Sciarretta, Giovanni Targher, and Francesco Violi
- Subjects
Blood Glucose ,Lipopolysaccharides ,Male ,0301 basic medicine ,endocrine system diseases ,P-selectin ,Endocrinology, Diabetes and Metabolism ,Medicine (miscellaneous) ,030204 cardiovascular system & hematology ,medicine.disease_cause ,Oxidized low density lipoprotein ,0302 clinical medicine ,impaired blood glucose ,Nutrition and Dietetics ,Zonulin ,Fasting ,Middle Aged ,Lipopolysaccharide ,Platelet activation ,Lipoproteins, LDL ,Female ,Cardiology and Cardiovascular Medicine ,Cholera Toxin ,medicine.medical_specialty ,liposaccarides ,platelet activation ,Permeability ,03 medical and health sciences ,Internal medicine ,Diabetes mellitus ,Glucose Intolerance ,medicine ,Humans ,Protein Precursors ,Aged ,Chi-Square Distribution ,Haptoglobins ,business.industry ,Case-control study ,nutritional and metabolic diseases ,medicine.disease ,Impaired fasting glucose ,Endotoxemia ,Gastrointestinal Tract ,Oxidative Stress ,Cross-Sectional Studies ,030104 developmental biology ,Endocrinology ,Case-Control Studies ,Multivariate Analysis ,Linear Models ,business ,Body mass index ,Biomarkers ,Oxidative stress - Abstract
Impaired fasting glucose (IFG) is associated with an increased risk of cardiovascular disease but the underlying mechanisms are still unclear. Aim of the study was to investigate the interplay between platelet activation, lipopolysaccharides (LPS) and markers of oxidative stress in patients with IFG and control subjects.We performed a cross-sectional study including 35 patients with IFG and 35 control subjects who were well comparable for age, sex, body mass index and smoking history. Serum levels of LPS, zonulin (a marker of gut permeability), oxidized LDL and plasma levels of soluble P-selectin, were measured. Patients with IFG had significantly higher levels of sP-selectin, LPS, zonulin and oxLDL compared to control subjects. The IFG status (beta coefficient: 0.518, p 0.001), higher LPS (beta coefficient: 0.352, p = 0.001) and female sex (beta coefficient: 0.179, p = 0.042) were independently associated with higher sP-selectin; in addition, oxLDL was positively associated with sP-selectin (r = 0.530, p 0.001) and LPS (r = 0.529, p = 0.001). In IFG patients, we found a significant association between LPS and zonulin (r = 0.521, p = 0.001); this association was confirmed at multivariable analysis (beta coefficient: 0.512, p = 0.007).Our study provides evidence that patients with IFG have increased platelet activation, and suggests LPS as a potential trigger for in vivo platelet activation in this patient population.
- Published
- 2017
45. Relationship of PCSK9 and Urinary Thromboxane Excretion to Cardiovascular Events in Patients With Atrial Fibrillation
- Author
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Daniele Pastori, Cristina Nocella, Alessio Farcomeni, Simona Bartimoccia, Maria Santulli, Fortunata Vasaturo, Roberto Carnevale, Danilo Menichelli, Francesco Violi, Pasquale Pignatelli, Mirella Saliola, Marco Antonio Casciaro, Domenico Ferro, Tommasa Vicario, Fabiana Albanese, Francesco Cribari, Alberto Paladino, Francesco Del Sole, Marta Novo, Vittoria Cammisotto, Paola Andreozzi, Tiziana Di Stefano, Patrizia Iannucci, and Elio Sabbatini
- Subjects
Male ,medicine.medical_specialty ,animal structures ,Thromboxane ,Urinary system ,030204 cardiovascular system & hematology ,Risk Assessment ,03 medical and health sciences ,0302 clinical medicine ,Predictive Value of Tests ,Internal medicine ,Atrial Fibrillation ,medicine ,risk factors ,Humans ,Prospective Studies ,030212 general & internal medicine ,Myocardial infarction ,Stroke ,Aged ,business.industry ,cholesterol ,myocardial infarction ,platelets ,stroke ,Cardiovascular Diseases ,Female ,Incidence ,Proprotein Convertase 9 ,Thromboxane B2 ,Cardiology and Cardiovascular Medicine ,PCSK9 ,fungi ,Atrial fibrillation ,medicine.disease ,Proprotein convertase ,Cardiology ,Kexin ,Settore SECS-S/01 - Statistica ,business - Abstract
Background: Soluble proprotein convertase subtilisin/kexin type 9 (PCSK9) has been shown to be predictive of cardiovascular events (CVEs) in patients who are at high cardiovascular risk. No...
- Published
- 2017
46. Insights into the origin of parthenogenesis in oligochaetes: Strong genetic structure in a cosmopolitan earthworm is not related to reproductive mode
- Author
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Gonzalo Giribet, Daniel Fernández Marchán, Rosa Fernández, Marta Novo, Irene de Sosa, and Darío J. Díaz Cosín
- Subjects
0106 biological sciences ,0301 basic medicine ,Genetics ,Mitochondrial DNA ,education.field_of_study ,biology ,Haplotype ,Population ,Soil Science ,Parthenogenesis ,010603 evolutionary biology ,01 natural sciences ,Microbiology ,Genetic divergence ,03 medical and health sciences ,030104 developmental biology ,Evolutionary biology ,Insect Science ,28S ribosomal RNA ,biology.animal ,Genetic structure ,Lumbricidae ,education - Abstract
Little is known about the origin and spatial pattern of the common phenomenon of parthenogenesis in earthworms. Aporrectodea trapezoides is unique in having a global distribution, where asexual forms are broadly known. Here we report two populations in Plasencia (Spain) and Karkra (Algeria) with multiple instances of co-occurrence of sexual and parthenogenetic individuals. We thus studied these populations where the two stages coexist with the aim of understanding the origins of parthenogenesis in earthworms. Two mitochondrial (COII and 16S rRNA) and three nuclear (ITS2, histone H3 and 28S rRNA) genes where evaluated to shed light on the mode, timing and frequency of origin of parthenogenetic forms. A population genetic study showed that in all cases sexual and parthenogenetic earthworms shared mitochondrial and nuclear sequence types, and that there is a strong genetic structure at the geographic level. Genetic divergence in both mitochondrial genes was high (up to 9.61% in COII and 6.12% in 16S rRNA), and ITS2 sequences were identical in individuals from closely related localities within a population. The haplotype networks, AMOVAs, Mantel tests and FST values all support the existence of strong genetic structure at the level of locality, but not due to reproductive mode, therefore providing evidence of multiple recent origins of parthenogenesis in this species.
- Published
- 2017
47. Is There an Interplay Between Adherence to Mediterranean Diet, Antioxidant Status, and Vascular Disease in Atrial Fibrillation Patients?
- Author
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Daniele Pastori, Francesco Violi, Danilo Menichelli, Pasquale Pignatelli, Roberto Carnevale, Marta Novo, Isabella Leo, Cristina Nocella, and Simona Bartimoccia
- Subjects
Male ,medicine.medical_specialty ,GPX3 ,Mediterranean diet ,atrial fibrillation ,cardiovascular events ,gluthatione peroxidase ,mediterranean diet ,physiology ,biochemistry ,molecular biology ,clinical biochemistry ,cell biology ,Physiology ,Clinical Biochemistry ,030204 cardiovascular system & hematology ,Diet, Mediterranean ,Biochemistry ,Gastroenterology ,Superoxide dismutase ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,Humans ,Prospective Studies ,Vascular Diseases ,030212 general & internal medicine ,Prospective cohort study ,Molecular Biology ,Aged ,General Environmental Science ,Aged, 80 and over ,Glutathione Peroxidase ,biology ,Superoxide Dismutase ,Proportional hazards model ,Vascular disease ,business.industry ,Incidence ,Incidence (epidemiology) ,Atrial fibrillation ,Cell Biology ,medicine.disease ,Surgery ,Treatment Outcome ,biology.protein ,Patient Compliance ,Regression Analysis ,General Earth and Planetary Sciences ,Female ,business - Abstract
Mediterranean Diet (Med-Diet) is associated with reduced incidence of vascular events (VEs) in atrial fibrillation (AF), but the mechanism accounting for its beneficial effect is only partially known. We hypothesized that Med-Diet may reduce VEs by improving antioxidant status, as assessed by glutathione peroxidase 3 (GPx3) and superoxide dismutase (SOD). We performed a prospective cohort study investigating the relationship between adherence to Med-Diet, serum baseline GPx3 and SOD activities, and the occurrence of VEs in 690 AF patients. GPx3 activity was directly associated with Med-Diet score (B = 0.192, p 0.001) and inversely with age (B = -0.124, p = 0.001), after adjustment for potential confounders; Med-Diet weakly affected SOD levels. During a mean follow-up of 46.1 ± 28.2 months, 89 VEs were recorded; patients with VEs had lower GPx3 levels compared with those without VEs (p = 0.002); and no differences regarding SOD activity were found. Multivariable Cox regression analysis showed that age (Hazard ratio [HR]:1.065, p 0.001), logGPx3 (above median, HR: 0.629, p 0.05), and Med-Diet score (HR: 0.547, p 0.05) predicted VEs. Med-Diet favorably modulates antioxidant activity of GPx3 in AF, resulting in reduced VEs rate. We hypothesize that the modulation of GPx3 levels by Med-Diet could represent an additional nutritional strategy to prevent VEs in AF patients. Antioxid. Redox Signal. 25, 751-755.
- Published
- 2016
48. Why are we blind to cryptic species? Lessons from the eyeless
- Author
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Daniel Fernández Marchán, Darío J. Díaz Cosín, and Marta Novo
- Subjects
0106 biological sciences ,Species complex ,Soil biology ,Soil Science ,Biota ,04 agricultural and veterinary sciences ,Reproductive isolation ,Biology ,010603 evolutionary biology ,01 natural sciences ,Microbiology ,Taxon ,Animal groups ,Evolutionary biology ,Insect Science ,040103 agronomy & agriculture ,0401 agriculture, forestry, and fisheries - Abstract
Molecular techniques have accelerated the detection of cryptic diversity within different animal groups, especially in soil fauna. These cryptic taxa show no morphological differences, but other traits vary between them. Research on earthworms has shown different approaches to identify biologically relevant differences between cryptic lineages, which can guide studies on other animals. These can be summarized in ecological preferences, unexplored morphological characters, behavior (and reproductive isolation), further molecular markers, genomic plasticity and epigenetics, and associated biota. These differences highlight the importance of characterizing cryptic genetic lineages in basic and applied research.
- Published
- 2018
49. Global distribution of earthworm diversity
- Author
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André L.C. Franco, Michiel Rutgers, Miguel Á. Rodríguez, Thomas W. Crowther, Helen Phillips, Tunsisa T. Hurisso, Olaf Schmidt, Matthew W. Warren, Shishir Paudel, Michael B. Wironen, Mónica Gutiérrez López, Basil V. Iannone, Aidan M. Keith, Amy Choi, Esperanza Huerta Lwanga, Boris Schröder, Olga Ferlian, Jaswinder Singh, Javier Rodeiro-Iglesias, Martin Holmstrup, Geoff H. Baker, Yahya Kooch, Rosa Fernández, Konstantin B. Gongalsky, Lindsey Norgrove, Eric Blanchart, Madhav P. Thakur, Felicity Crotty, Steven J. Vanek, Thomas Bolger, Jan Hendrik Moos, Guillaume Xavier Rousseau, Marie Luise Carolina Bartz, Iñigo Virto, Michael Steinwandter, Kelly S. Ramirez, Veikko Huhta, Ulrich Brose, Michel Loreau, Davorka K. Hackenberger, David J. Russell, Loes van Schaik, Salvador Rebollo, Jonatan Klaminder, Gerardo Moreno, Benjamin Schwarz, Julia Seeber, Yvan Capowiez, David A. Wardle, Bernd Blossey, Franciska T. de Vries, Christoph Emmerling, Robert L. Bradley, Courtland Kelly, Liliana B. Falco, Alexander M. Roth, Michael J. Gundale, Radim Matula, Andrea Dávalos, Lorenzo Pérez-Camacho, Johan Neirynck, Monika Joschko, Marta Novo, Dolores Trigo, Jérôme Mathieu, Adrian A. Wackett, Anne W. de Valença, Elizabeth M. Bach, Daniel R. Lammel, Devin Routh, Madalina Iordache, Luis M. Hernández, Johan Pansu, Juan B. Jesús Lidón, Alejandro Morón-Ríos, Maxim Shashkov, Ehsan Sayad, Martine Fugère, Nobuhiro Kaneko, Mark E. Caulfield, Klaus Birkhofer, Wim H. van der Putten, Iurii M. Lebedev, Alberto Orgiazzi, Miwa Arai, H. Lalthanzara, Raphaël Marichal, Andrew R. Holdsworth, Steven J. Fonte, Maria J. I. Briones, Raúl Piñeiro, Jean-François Ponge, Nick van Eekeren, Takuo Hishi, Julia Krebs, Joanne M. Bennett, George G. Brown, Birgitta König-Ries, Carlos Fragoso, Victoria Nuzzo, Anna Rożen, Scott R. Loss, Bart Muys, Bryant C. Scharenbroch, Michael Schirrmann, Radoslava Kanianska, Irina V. Zenkova, Maria Kernecker, Abegail T Fusilero, Sandy M. Smith, Shaieste Gholami, Robin Beauséjour, Mac A. Callaham, Nathaniel H. Wehr, Yiqing Li, Kristine N. Hopfensperger, Mujeeb Rahman P, Andrés Esteban Duhour, Erin K. Cameron, Diana H. Wall, Muhammad Rashid, José Antonio Talavera, Matthias C. Rillig, Armand W. Koné, Johan van den Hoogen, Darío J. Díaz Cosín, Anahí Domínguez, Thibaud Decaëns, Fredrick O. Ayuke, Carlos A. Guerra, Guénola Pérès, Volkmar Wolters, Jiro Tsukamoto, Nico Eisenhauer, José Camilo Bedano, Weixin Zhang, Noa Kekuewa Lincoln, Visa Nuutinen, Joann K. Whalen, Christian Mulder, Sanna T. Kukkonen, German Centre for Integrative Biodiversity Research (iDiv), Universidade Positivo, Departamento de Ecología y Biología Animal, Universidad de Vigo, Crowther Lab, Institute of Integrative Biology, ETH Zürich, A.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences [Moscow] (RAS), JRC Institute for Environment and Sustainability (IES), European Commission - Joint Research Centre [Ispra] (JRC), Department of Biology [Fort Collins], Colorado State University [Fort Collins] (CSU), German Centre for Integrative Biodiversity Research, Étude et compréhension de la biodiversité (ECODIV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Friedrich-Schiller-Universität Jena, Station d'écologie théorique et expérimentale (SETE), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Observatoire Midi-Pyrénées (OMP), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Institut d'écologie et des sciences de l'environnement de Paris (IEES), Centre National de la Recherche Scientifique (CNRS)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Recherche Agronomique (INRA), Department of Terrestrial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), Berlin-Brandenburg Institute of Advanced Biodiversity Research (BBIB), National Institute for Public Health and the Environment [Bilthoven] (RIVM), Institut de recherche pour le développement [IRD] : UMR210, School of Biology and Environmental Science, University College Dublin [Dublin] (UCD), Unité de recherche Plantes et Systèmes de Culture Horticoles (PSH), Institut National de la Recherche Agronomique (INRA), Centro International de Agricultura Tropical, Red de Biodiversidad y Sistemática, Instituto de Ecología, Department of Terrestrial Ecology, Fluides, automatique, systèmes thermiques (FAST), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Biogéochimie et écologie des milieux continentaux (Bioemco), Centre National de la Recherche Scientifique (CNRS)-AgroParisTech-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Paris (ENS Paris), Universidad de Extremadura (UEX), Division of Forest, Nature and Landscape, Department of Earth and Environmental Sciences [Leuven] (EES), Catholic University of Leuven - Katholieke Universiteit Leuven (KU Leuven)-Catholic University of Leuven - Katholieke Universiteit Leuven (KU Leuven), Laboratoire d'Ecologie Alpine (LECA), Université Grenoble Alpes (UGA)-Université Joseph Fourier - Grenoble 1 (UJF)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS), Sol Agro et hydrosystème Spatialisation (SAS), AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA), University of Vigo [ Pontevedra], Centre d'Ecologie et des Sciences de la COnservation (CESCO), Muséum national d'Histoire naturelle (MNHN)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Center of Excellence in Environmental Studies, King Abdulaziz University, Dept Ciencias Vida, Universidad de Alcalá - University of Alcalá (UAH), Faculty of Business Administration and Economics, DS&OR-Lab, University of Paderborn, Laboratoire d'oncogénétique moléculaire, Mountain Agriculture Research Unit, University of Innsbruck, Independent, Universidad Pública de Navarra [Espagne] (UPNA), McGill University, Department of Animal Ecology and Systematics, Justus-Liebig-Universität Gießen (JLU), Jiangsu Academy of Agricultural Sciences, Université de Leipzig, Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Leipzig University, Martin-Luther-Universität Halle Wittenberg (MLU), Universidade de Vigo, Embrapa Forestry, Brazilian Agricultural Research Corporation (Embrapa), Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich), M.V. Lomonosov Moscow State University, Department of Biometry and Environmental System Analysis, Albert-Ludwigs-Universität Freiburg, Institute of Biodiversity, Friedrich Schiller University Jena, Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Université Paul-Valéry - Montpellier 3 (UPVM)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institute of Computer Science, Friedrich Schiller University Jena, Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France -Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France -Centre National de la Recherche Scientifique (CNRS), Institut d'écologie et des sciences de l'environnement de Paris (iEES), Institut National de la Recherche Agronomique (INRA)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS), Università degli studi di Catania = University of Catania (Unict), Department of Terrestrial Ecology [Wageningen], Netherlands Institute of Ecology (NIOO-KNAW), Laboratory of Nematology, Department of Plant Sciences, Wageningen University & Research, Freie Universität Berlin, Senckenberg Museum of Natural History Görlitz, Senckenberg – Leibniz Institution for Biodiversity and Earth System Research - Senckenberg Gesellschaft für Naturforschung, Leibniz Association-Leibniz Association, Global Soil Biodiversity Initiative and School of Global Environmental Sustainability, Colorado State University, Asian School of the Environment (ASE), Nanyang Technological University [Singapour], Institute for Agro-Environmental Sciences, National Agriculture and Food Research Organization, Department of Land Resource Management and Agricultural technology (LARMAT), College of 80 Agriculture and Veterinary Sciences, University of Nairobi (LARMAT), CSIRO Health & Biosecurity, Département de biologie [Sherbrooke] (UdeS), Faculté des sciences [Sherbrooke] (UdeS), Université de Sherbrooke (UdeS)-Université de Sherbrooke (UdeS), Geology Department, FCEFQyN, ICBIA-CONICET (National Scientific and Technical Research Council), National University of Río Cuarto, Department of Ecology, Brandenburg University of Technology, Ecologie fonctionnelle et biogéochimie des sols et des agro-écosystèmes (UMR Eco&Sols), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Department of Natural Resources, Cornell University, Cornell University [New York], UCD Earth Institute, University College Dublin, USDA Forest Service, Southern Research Station, Environnement Méditerranéen et Modélisation des Agro-Hydrosystèmes (EMMAH), Institut National de la Recherche Agronomique (INRA)-Avignon Université (AU), Farming Systems Ecology, Wageningen University and Research, Faculty of Forestry, University of Toronto, Institute of Biological, Environmental & Rural Sciences, Aberystwyth University, Department of Biological Sciences, SUNY Cortland, Department of Biodiversity, Ecology and Evolution [Madrid], Universidad Complutense de Madrid = Complutense University of Madrid [Madrid] (UCM), Laboratorio de Ecología, Instituto de Ecología y Desarrollo Sustentable, Universidad Nacional de Luján, Louis Bolk Institute (LBI), Department of Soil Science, Faculty of Regional & Environmental Sciences, University of Trier, Ciencias Básicas, Instituto de Ecología y Desarrollo Sustentable -INEDES, Universidad Nacional de Lujan, Institut de Biologia Evolutiva [Barcelona] (IBE / UPF - CSIC), Universitat Pompeu Fabra [Barcelona] (UPF), Department of Soil and Crop Sciences, Colorado State University, Biodiversity and Systematic Network, Instituto de Ecología A.C., Department of Biological Science and Environmental Studies, University of the Philippines - Mindanao, Natural Resources Department, Razi University, Department of Forest Ecology and Management, Swedish University of Agricultural Sciences (SLU), Department of Biology, J. J. Strossmayer University of Osijek, Agricultural Engineering, Postgraduate Program in Agroecology, Maranhão State University, Faculty of Agriculture, Kyushu University, Minnesota Pollution Control Agency, Department of Bioscience, Aarhus University, Biological Sciences, Northern Kentucky University, Agricultura Sociedad y Ambiente, El Colegio de la Frontera Sur, Soil Physics and Land Management degradation, Wageningen University & Research, Wageningen University, Department of Biological and Environmental Science [Jyväskylä Univ] (JYU), University of Jyväskylä (JYU), College of Agriculture, Environmental and Human Sciences, Lincoln University of Missouri, School of Forest Resources and Conservation [Gainesville] (UF|IFAS|FFGS), Institute of Food and Agricultural Sciences [Gainesville] (UF|IFAS), University of Florida [Gainesville] (UF)-University of Florida [Gainesville] (UF), Sustainable Development and Environment Engineering, Banat's University of Agricultural Sciences and Veterinary Medicine 'King Michael the 1st of Romania', Experimental Infrastructure Platform, Leibniz Centre for Agricultural Landscape Research (ZALF), Faculty of Food and Agricultural Sciences, Fukushima University, Matej Bel University (UMB), Centre for Ecology and Hydrology [Lancaster] (CEH), Natural Environment Research Council (NERC), Land Use and Governance, Leibniz Centre for Agricultural Landscape Research (ZALF), Climate Impacts Research Centre (CIRC), Umeå University, UR Gestion Durable des Sols, UFR Sciences de la Nature, Université Nangui Abrogoua, Tarbiat Modares University [Tehran], Natural Resources Institute Finland (LUKE), Department of Zoology, Pachhunga University College, Soil Science, ESALQ-USP, Universidade de São Paulo, College of Agriculture, Forestry and Natural Resource Management, University of Hawaii at Hilo, Tropical Plant and Soil Sciences, College of Tropical Agriculture and Human Resources, University of Hawai‘i at Mānoa, Department of Natural Resource Ecology and Management, Oklahoma State University, Performance des systèmes de culture des plantes pérennes (UPR Système de pérennes), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Department of Forest Ecology, Faculty of Forestry and Wood Sciences, Czech University of Life Sciences Prague (CZU), Department of Soil and Environment, Forest Research Institute of Baden-Wuerttemberg, Thuenen-Institute of Organic Farming, Forestry School - INDEHESA, University of Extremadura, Conservación de la Biodiversidad, El Colegio de la Frontera Sur, Catholic University of Leuven - Katholieke Universiteit Leuven (KU Leuven), Research Institute for Nature and Forest, Research Institute for Nature and Forest (INBO), School of Agricultural, Forest and Food Sciences, Bern University of Applied Sciences (BFH), Natural Area Consultants, Department of Zoology, Pocker Sahib Memorial Orphanage College, CSIRO Ocean & Atmosphere, CSIRO, Adaptation et diversité en milieu marin (AD2M), Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Ecology and Forest Restoration Group, Department of Life Sciences, University of Alcalá, Computing, ESEI, Vigo, Mécanismes Adaptatifs et Evolution (MECADEV), Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS), Centre of Excellence in Environmental Studies, King Abdulaziz University, Environmental Sciences, COMSATS University, Life Sciences, Sciences Faculty, University of Alcalá, University of Minnesota [Twin Cities] (UMN), University of Minnesota System, Friends of the Mississippi River, Postgraduate Program in Biodiversity and Conservation, Federal University of Maranhão, Uniwersytet Jagielloński w Krakowie = Jagiellonian University (UJ), Institute of Ecology, Technical University of Berlin, College of Natural Resources, University of Wisconsin, Engineering for Crop Production, Leibniz Institute for Agricultural Engineering and Bioeconomy (ATB), UCD School of Agriculture and Food Science, University College Dublin, Landscape Ecology and Environmental Systems Analysis, Institute of Geoecology, Technische Universität Braunschweig, Universität Innsbruck [Innsbruck], Institute for Alpine Environment, Eurac Research, Laboratory of Ecosystem Modelling, Institute of Physicochemical and Biological Problems in Soil Sciences, Russian Academy of Science, Laboratory of Computational Ecology, Institute of Mathematical Problems of Biology RAS – the Branch of Keldysh Institute of Applied Mathematics of Russian Academy of Sciences, Post Graduate Department of Zoology, Khalsa College Amritsar, John H. Daniels Faculty of Architecture, Landscape and Design, University of Toronto, Universidad de La Laguna [Tenerife - SP] (ULL), Faculty of Agriculture, Kochi University, Food & Agriculture, WWF-Netherlands, Universidad Pública de Navarra [Espagne] = Public University of Navarra (UPNA), Soil, Water and Climate, University of Minnesota, Earth Innovation Institute, Department of Natural Resources & Environmental Management, University of Hawai'i at Mānoa, Natural Resource Sciences, McGill University, McGill University = Université McGill [Montréal, Canada], The Nature Conservancy, Justus-Liebig-Universität Gießen = Justus Liebig University (JLU), Laboratory of Terrestrial Ecosystems, Kola Science Centre, Institute of the North Industrial Ecology Problems, Key Laboratory of Geospatial Technology for the Middle and Lower Yellow River Regions (Henan University), Ministry of Education, College of Environment and Planning, Henan University, Department of Environmental Science, Saint Mary’s University, Faculty of Biological and Environmental Sciences [Helsinki], Helsingin yliopisto = Helsingfors universitet = University of Helsinki, ANR-11-IDEX-0002,UNITI,Université Fédérale de Toulouse(2011), Institute of Biology, Leipzig University, Institute of Biology, Martin Luther University Halle-Wittenberg, Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Université Paul-Valéry - Montpellier 3 (UPVM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut de Recherche pour le Développement (IRD [France-Sud]), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Observatoire Midi-Pyrénées (OMP), Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD), Department of Biological, Geological and Environmental Sciences, University of Catania, Institute of Biology, Freie Universität Berlin, Senckenberg Museum for Natural History Görlitz, Department of Soil Zoology, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Faculty of Natural Resources & Marine Sciences, Tarbiat Modares University, Production Systems, Horticulture Technologies, Natural Resources Institute Finland, Soil Ecosystems, Natural Resources Institute Finland (Luke), Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA), Departamento de Informática, Escuela Superior de Ingeniería Informática, Universidad de Vigo, Institute of Environmental Sciences, Jagiellonian University, Department of Ecology, University of Innsbruck, Department of Animal Biology (Zoology area), Science Faculty, University of La Laguna, Dpto. Ciencias, IS-FOOD, Universidad Pública de Navarra, Department of Animal Ecology, Justus Liebig University, University of Helsinki, German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Department of Biology, Colorado State University, Centre National de la Recherche Scientifique (CNRS)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Joseph Fourier - Grenoble 1 (UJF)-Université Grenoble Alpes (UGA), Lomonosov Moscow State University (MSU), Friedrich-Schiller-Universität = Friedrich Schiller University Jena [Jena, Germany], Université Paul-Valéry - Montpellier 3 (UPVM)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École Pratique des Hautes Études (EPHE), Université de Toulouse (UT)-Université de Toulouse (UT)-Observatoire Midi-Pyrénées (OMP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France -Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France -Centre National de la Recherche Scientifique (CNRS), Wageningen University and Research [Wageningen] (WUR), National Agriculture and Food Research Organization (NARO), University of Nairobi (UoN), Commonwealth Scientific and Industrial Research Organisation [Canberra] (CSIRO), National University of Río Cuarto = Universidad Nacional de Río Cuarto (UNRC), Brandenburg University of Technology [Cottbus – Senftenberg] (BTU), Department of Natural Resources & The Environment [CALS], College of Agriculture and Life Sciences [Cornell University] (CALS), Cornell University [New York]-Cornell University [New York], School of Biology and Environmental Sciences (SBES), USDA Forest Service, University of Toronto, Aberystwyth University, Universidad Nacional de Luján [Buenos Aires], Trier University of Applied Sciences, Razi University of Kermanshah, Josip Juraj Strossmayer University of Osijek, Universidade Estadual do Maranhão = State University of Maranhão (UEMA), Kyushu University, Aarhus University [Aarhus], Northern Kentucky University, Adaptation et diversité en milieu marin (ADMM), Institut national des sciences de l'Univers (INSU - CNRS)-Station biologique de Roscoff (SBR), Ecosystem and Landscape Dynamics (IBED, FNWI), Deutsche Forschungsgemeinschaft - German Research Foundation (DFG), Martin-Luther-University Halle-Wittenberg, Joint Research Centre, European Commission, Department of Biology, University of Minho [Braga], University of Amsterdam [Amsterdam] (UvA), Institut d'écologie et des sciences de l'environnement de Paris (iEES Paris), Institut National de la Recherche Agronomique (INRA)-Sorbonne Université (SU)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS), Royal Netherlands Academy of Arts and Sciences (KNAW), Laboratory of Nematology, Department of Plant Sciences, Free University of Berlin (FU), Senckenberg Research Institute, European Project: 227161,EC:FP7:KBBE,FP7-KBBE-2008-2B,BIOBIO(2009), Biometry and Environmental System Analysis, University of Freiburg, Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Paul-Valéry - Montpellier 3 (UM3), Theoretical and Experimental Ecology Station, Centre National de la Recherche Scientifique (CNRS), Asian School of the Environment, Nanyang Technological University, Département de Biologie, Université de Sherbrooke, Sherbrooke, QC, Canada, Université de Sherbrooke, Institut National de la Recherche Agronomique (INRA)-Institut de Recherche pour le Développement (IRD)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Cornell University, UMR 1114 'EMMAH', INRA, Complutense University of Madrid (UCM), Institute of Evolutionary Biology (CSIC-Universitat Pompeu Fabra), Forest Ecology and Management, Swedish University of Agricultural Sciences, Department of Biological and Environmental Science, University of Jyväskylä, School of Forest Resources and Conservation, University of Florida, Department of Environmental Management, Faculty of Natural Sciences, Matej Bel University, Department of Ecology and Environmental Science, Climate Impacts Research Centre, Umeå University, A.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Performance des systèmes de culture des plantes pérennes (Cirad-Persyst-UPR 34 Système de pérennes), Département Performances des systèmes de production et de transformation tropicaux (Cirad-PERSYST), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Department of Forest Ecology, Faculty of Forestry and Wood Sciences, Czech University of Life Sciences Prague, Department of Earth & Environmental Sciences, KU Leuven, School of Agricultural, Forest and Food Sciences, Bern University of Applied Sciences, UMR7144 Adaptation et Diversité en Milieu Marin, Station Biologique de Roscoff, CNRS-Sorbonne Universite, AGROCAMPUS OUEST-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Mécanismes adaptatifs : des organismes aux communautés (MECADEV), Department of Forest Resources, University of Minnesota, Université Paul-Valéry - Montpellier 3 (UM3)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École pratique des hautes études (EPHE)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), European Research Council, Academy of Finland, Natural Sciences and Engineering Research Council of Canada, Russian Foundation for Basic Research, Slovak Research and Development Agency, Wageningen University and Research Centre, International Atomic Energy Agency, Fundação de Amparo à Pesquisa do Estado de São Paulo, Royal Canadian Geographical Society, Irish Government, University of Hawaii, U.S. Navy, Department of Science and Technology (India), Department of Defense (US), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Ministry of Education, Youth and Sports (Czech Republic), Agence Nationale de la Recherche (France), Austrian Science Fund, Welsh Government, Science Foundation Ireland, University of Kentucky, Higher Education Commission (Pakistan), Universidad Complutense de Madrid, Grains Research and Development Corporation (Australia), Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina), Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Ministère de l'Europe et des Affaires étrangères (France), University of Minho, Università degli Studi di Catania (UniCT), Terrestrial Ecology (TE), Institut de Recherche pour le Développement (IRD)-Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Université Fédérale Toulouse Midi-Pyrénées-Institut de Recherche pour le Développement (IRD)-Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)
- Subjects
0106 biological sciences ,LITTER ,Earth, Planet ,Climate ,Biologie du sol ,Biodiversity ,Facteur climatique ,01 natural sciences ,Ver de terre ,Ecosystem services ,purl.org/becyt/ford/1 [https] ,Soil ,Abundance (ecology) ,FORESTS ,11. Sustainability ,DRIVERS ,Biomass ,2. Zero hunger ,0303 health sciences ,Biomass (ecology) ,Multidisciplinary ,biology ,Ecology ,Earth ,04 agricultural and veterinary sciences ,POPULATION-DENSITY ,earthworm ,distribution ,diversity ,pattern ,PE&RC ,MINHOCAS ,Habitat ,Plant Production Systems ,international ,L20 - Écologie animale ,Biodiversité ,Écosystème ,Zone tropicale ,Soil biology ,Biocénose ,Invertebrados ,[SDV.SA.SDS]Life Sciences [q-bio]/Agricultural sciences/Soil study ,Models, Biological ,010603 evolutionary biology ,Ecology and Environment ,Zone tempérée ,03 medical and health sciences ,[SDV.EE.ECO]Life Sciences [q-bio]/Ecology, environment/Ecosystems ,Life Science ,Animals ,Ecosystem ,PLANT ,Oligochaeta ,purl.org/becyt/ford/1.6 [https] ,030304 developmental biology ,Earthworm ,Tropics ,P34 - Biologie du sol ,Species diversity ,SHIFTS ,Farm Systems Ecology Group ,Bodemfysica en Landbeheer ,15. Life on land ,Ecología ,biology.organism_classification ,Soil Physics and Land Management ,SOIL ,Agriculture and Soil Science ,13. Climate action ,Plantaardige Productiesystemen ,040103 agronomy & agriculture ,PATTERNS ,Linear Models ,0401 agriculture, forestry, and fisheries ,BIODIVERSITY ,Species richness ,Planet ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology ,COMMUNITIES ,Zoology ,Animal Distribution ,Models Biological - Abstract
Soil organisms, including earthworms, are a key component of terrestrial ecosystems. However, little is known about their diversity, their distribution, and the threats affecting them. We compiled a global dataset of sampled earthworm communities from 6928 sites in 57 countries as a basis for predicting patterns in earthworm diversity, abundance, and biomass. We found that local species richness and abundance typically peaked at higher latitudes, displaying patterns opposite to those observed in aboveground organisms. However, high species dissimilarity across tropical locations may cause diversity across the entirety of the tropics to be higher than elsewhere. Climate variables were found to be more important in shaping earthworm communities than soil properties or habitat cover. These findings suggest that climate change may have serious implications for earthworm communities and for the functions they provide., 677232 to N.E.). K.S.R. and W.H.v.d.P. were supported by ERC-ADV grant 323020 to W.H.v.d.P. Also supported by iDiv (DFG FZT118) Flexpool proposal 34600850 (C.A.G. and N.E.); the Academy of Finland (285882) and the Natural Sciences and Engineering Research Council of Canada (postdoctoral fellowship and RGPIN-2019-05758) (E.K.C.); DOB Ecology (T.W.C., J.v.d.H., and D.R.); ERC-AdG 694368 (M.R.); and the TULIP Laboratory of Excellence (ANR-10-LABX-41) (M.L.). In addition, data collection was funded by the Russian Foundation for Basic Research (12-04-01538-a, 12-04-01734-a, 14-44-03666-r_center_a, 15-29-02724-ofi_m, 16-04-01878-a 19-05-00245); Tarbiat Modares University; Aurora Organic Dairy; UGC(NERO) (F. 1-6/Acctt./NERO/2007-08/1485); Natural Sciences and Engineering Research Council (RGPIN-2017-05391); Slovak Research and Development Agency (APVV-0098-12); Science for Global Development through Wageningen University; Norman Borlaug LEAP Programme and International Atomic Energy Agency (IAEA); São Paulo Research Foundation - FAPESP (12/22510-8); Oklahoma Agricultural Experiment Station; INIA - Spanish Agency (SUM 2006-00012-00-0); Royal Canadian Geographical Society; Environmental Protection Agency (Ireland) (2005-S-LS-8); University of Hawai‘i at Mānoa (HAW01127H; HAW01123M); European Union FP7 (FunDivEurope, 265171); U.S. Department of the Navy, Commander Pacific Fleet (W9126G-13-2-0047); Science and Engineering Research Board (SB/SO/AS-030/2013) Department of Science and Technology, New Delhi, India; Strategic Environmental Research and Development Program (SERDP) of the U.S. Department of Defense (RC-1542); Maranhão State Research Foundation (FAPEMA); Coordination for the Improvement of Higher Education Personnel (CAPES); Ministry of Education, Youth and Sports of the Czech Republic (LTT17033); Colorado Wheat Research Foundation; Zone Atelier Alpes, French National Research Agency (ANR-11-BSV7-020-01, ANR-09-STRA-02-01, ANR 06 BIODIV 009-01); Austrian Science Fund (P16027, T441); Landwirtschaftliche Rentenbank Frankfurt am Main; Welsh Government and the European Agricultural Fund for Rural Development (Project Ref. A AAB 62 03 qA731606); SÉPAQ; Ministry of Agriculture and Forestry of Finland; Science Foundation Ireland (EEB0061); University of Toronto (Faculty of Forestry); National Science and Engineering Research Council of Canada; Haliburton Forest and Wildlife Reserve; NKU College of Arts and Sciences Grant; Österreichische Forschungsförderungsgesellschaft (837393 and 837426); Mountain Agriculture Research Unit of the University of Innsbruck; Higher Education Commission of Pakistan; Kerala Forest Research Institute, Peechi, Kerala; UNEP/GEF/TSBF-CIAT Project on Conservation and Sustainable Management of Belowground Biodiversity; Ministry of Agriculture and Forestry of Finland; Complutense University of Madrid/European Union FP7 project BioBio (FPU UCM 613520); GRDC; AWI; LWRRDC; DRDC; CONICET (National Scientific and Technical Research Council) and FONCyT (National Agency of Scientific and Technological Promotion) (PICT, PAE, PIP), Universidad Nacional de Luján y FONCyT [PICT 2293 (2006)], Fonds de recherche sur la nature et les technologies du Québec (131894), Deutsche Forschungsgemeinschaft [SCHR1000/3-1, SCHR1000/6-1, 6-2 (FOR 1598), WO 670/7-1, WO 670/7-2, and SCHA 1719/1-2], CONACYT (FONDOS MIXTOS TABASCO/PROYECTO11316); NSF (DGE-0549245, DGE-0549245, DEB-BE-0909452, NSF1241932); Institute for Environmental Science and Policy at the University of Illinois at Chicago; Dean’s Scholar Program at UIC; Garden Club of America Zone VI Fellowship in Urban Forestry from the Casey Tree Endowment Fund; J. E. Weaver Competitive Grant from the Nebraska Chapter of The Nature Conservancy; the College of Liberal Arts and Sciences at DePaul University; Elmore Hadley Award for Research in Ecology and Evolution from the UIC Dept. of Biological Sciences; Spanish CICYT (AMB96-1161; REN2000-0783/GLO; REN2003-05553/GLO; REN2003-03989/GLO; CGL2007-60661/BOS); Yokohama National University; MEXT KAKENHI (25220104); Japan Society for the Promotion of Science KAKENHI (25281053, 17KT0074, 25252026); ADEME (0775C0035); Ministry of Science, Innovation and Universities of Spain (CGL2017-86926-P); Syngenta Philippines; UPSTREAM; LTSER (Val Mazia/Matschertal); Marie Sklodowska Curie Postdoctoral Fellowship (747607); National Science and Technology Base Resource Survey Project of China (2018FY100306); McKnight Foundation (14-168); Program of Fundamental Researches of Presidium of Russian Academy of Sciences (AAAA-A18-118021490070-5); Brazilian National Council of Research CNPq; and French Ministry of Foreign and European Affairs. Author contributions: H.R.P.P. led the analysis, data curation, and writing of the original manuscript draft. C.A.G. assisted in analyses and writing of the original manuscript draft. E.K.C. and N.E. revised subsequent manuscript drafts. J.v.d.H., D.R., and T.W.C. provided additional analyses. E.K.C., N.E., and M.P.T. acquired funding for the project. J.K., K.B.G., B.S., M.L.C.B., M.J.I.B., and G.B. contributed to data curation. H.R.P.P., C.A.G., M.L.C.B., M.J.I.B., G.B., O.F., A.O., E.M.B., J.B., U.B., T.D., F.T.d.V., B.K.-R., M.L., J.M., C.M., W.H.v.d.P., K.S.R., M.C.R., D.R., M.R., M.P.T., D.H.W., D.A.W., E.K.C., and N.E. contributed to the project conceptualization. All authors reviewed and edited the final draft manuscript. The majority of the authors provided data for the analyses. Competing interests: The authors declare no competing interests. Data and materials availability: Data and analysis code are available on the iDiv Data repository (DOI: 10.25829/idiv.1804-5-2593) and GitHub (https://github.com/helenphillips/GlobalEWDiversity; DOI: 10.5281/zenodo.3386456).
- Published
- 2019
50. Delegating Sex: Differential Gene Expression in Stolonizing Syllids Uncovers the Hormonal Control of Reproduction
- Author
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Aida Verdes, Rosa Fernández, Gonzalo Giribet, Nathan J. Kenny, Patricia Álvarez-Campos, Ana Riesgo, and Marta Novo
- Subjects
0106 biological sciences ,Male ,Complement receptor 2 ,media_common.quotation_subject ,Invertebrados ,Gene Expression ,010603 evolutionary biology ,01 natural sciences ,Gametogenesis ,Transcriptome ,reproduction ,03 medical and health sciences ,Vitellogenin ,transcriptomics ,Gene expression ,Genetics ,Animals ,Hormonal control ,Metamorphosis ,Transcriptomics ,Gene ,Ecology, Evolution, Behavior and Systematics ,030304 developmental biology ,media_common ,0303 health sciences ,biology ,Reproduction ,Polychaeta ,Stolonizing syllids ,Hormones ,Cell biology ,Biología marina ,hormonal control ,biology.protein ,Female ,stolonizing syllids ,Research Article - Abstract
Stolonization in syllid annelids is a unique mode of reproduction among animals. During the breeding season, a structure resembling the adult but containing only gametes, called stolon, is formed generally at the posterior end of the animal. When stolons mature, they detach from the adult and gametes are released into the water column. The process is synchronized within each species, and it has been reported to be under environmental and endogenous control, probably via endocrine regulation. To further understand reproduction in syllids and to elucidate the molecular toolkit underlying stolonization, we generated Illumina RNA-seq data from different tissues of reproductive and nonreproductive individuals of Syllis magdalena and characterized gene expression during the stolonization process. Several genes involved in gametogenesis (ovochymase, vitellogenin, testis-specific serine/threonine-kinase), immune response (complement receptor 2), neuronal development (tyrosine-protein kinase Src42A), cell proliferation (alpha-1D adrenergic receptor), and steroid metabolism (hydroxysteroid dehydrogenase 2) were found differentially expressed in the different tissues and conditions analyzed. In addition, our findings suggest that several neurohormones, such as methyl farnesoate, dopamine, and serotonin, might trigger stolon formation, the correct maturation of gametes and the detachment of stolons when gametogenesis ends. The process seems to be under circadian control, as indicated by the expression patterns of r-opsins. Overall, our results shed light into the genes that orchestrate the onset of gamete formation and improve our understanding of how some hormones, previously reported to be involved in reproduction and metamorphosis processes in other invertebrates, seem to also regulate reproduction via stolonization. This research received funding from the European Union’s (European Atomic Energy Community’s) Seventh Framework Program (FP7/2007–2013; FP7/2007–2011) under grant agreement 227799 to P.A.-C. Sequencing and analyses were conducted with internal MCZ funds to G.G. and with the support of the Center for Systems Biology and the Research Computing group, both from the Faculty of Arts and Sciences (Harvard University), and with internal funds from the Department of Life Sciences (Natural History Museum of London) to AR. One anonymous reviewer and Associate Editor Mandë Holford provided constructive criticism which helped to improve this study.
- Published
- 2019
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