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Flowers have been commonly used in cooking to add color and flavor to dishes. In addition to enhancing the visual appeal of food, many edible flowers also contain bioactive compounds that promote good health. These compounds include antimicrobial, antihypertensive, nephroprotective, antiulcer, and anticancer agents. In the last 5 years, there have been 95 published reviews about edible flowers. Among these, 43% have concentrated on Food Science and Technology, while 32% have analyzed their effects on human health. Most of these edible flowers are commonly consumed, but some are less known due to limited distribution or seasonality. These lesser‐explored flowers often contain compounds that offer significant health advantages. Therefore, this review focuses on exploring the characteristics, phytochemical composition, and bioactive compounds found in less commonly examined edible flowers. The flowers included in this review are peonies, forget‐me‐nots, frangipani, alpine roses, wild roses, hibiscus species, common lilacs, woodland geraniums, camellias, Aztec marigolds, kiri flowers, sunflowers, yucca flower, hollyhocks, and cornflowers. Due to their diverse biological activities, these flowers provide various health benefits and can be used to be incorporated into food and supplements or develop mainly cancer‐fighting medications. [ABSTRACT FROM AUTHOR]

Chambers of Rhetoric in Flanders, Brabant, Holland and Zeeland opened themselves up to the new religious ideas, especially in the 1530s. These were discussed among their members and the Scriptures were read and conversed upon. The results of their reflections were written down and performed in allegorical plays (spelen van sinne), biblical plays, refrains and songs. In this essay particular attention will be paid to how the staging of biblical figures and stories was not only a means to propagate the new "evangelical" ideas among the audience, but also a way to criticize the persecution of the same by the imperial and ecclesiastical authorities. To that purpose a closer analysis is offered of a play entitled De Bekeeringe Pauli (The Conversion of Paul), which was based upon Acts 9:1–19. Most probably the play was created by the Chamber of Rhetoric De Goudbloem (Marigold), which was active in Vilvoorde near Brussels. The essay will further illustrate how these "religiously-politically incorrect" plays induced the authorities to take increasingly stringent measures against the performance of rhetoricians' plays in the Low Countries. [ABSTRACT FROM AUTHOR]

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of lutein‐rich extract of Tagetes erecta L. as sensory additive (functional group: Colourants (ii) substances which, when fed to animals, add colours to food of animal origin) for turkeys for fattening. The additive is already authorised for use in feed for chickens for fattening and minor poultry for fattening and laying hens and minor poultry for laying. The FEEDAP Panel concluded that the use of lutein‐rich extract of T. erecta is safe for turkeys for fattening when used up to the maximum proposed use level of 80 mg total carotenoids/kg complete feed. The Panel concluded that the use of lutein‐rich extract of T. erecta in feed for turkeys for fattening under the proposed conditions of use would not be of concern for the consumer, considering also its use in other poultry for fattening and for laying hens. Regarding user safety, the lutein‐rich extract of T. erecta extract is irritant to skin and eyes and any exposure is considered a risk. The conclusions on user safety reached for the lutein‐rich extract of T. erecta would, in principle, apply to preparations made with it. The use of the additive in feed for turkeys for fattening under the proposed conditions of use is safe for the environment. The FEEDAP Panel concluded that the additive has the potential to colour the skin of turkeys for fattening at the proposed conditions of use. [ABSTRACT FROM AUTHOR]

In a long-term monoculture with fruit trees and tree nurseries, it is necessary to regenerate the soil due to the risk of apple replant disease (ARD). The occurrence of ARD is manifested in the structure of the mycobiome. The assumption of our experiment was that the use of oil radish (Raphanus sativus var. oleifera), white mustard (Sinapis alba), and marigold (Tagetes patula L.) as phytosanitary plants for biofumigation would provide crops with nutrients, improve soil physicochemical properties, and influence the diversity of microbiota, including fungal networks, towards a beneficial mycobiome. Metagenomic analysis of fungal populations based on the hypervariable ITS1 region was used for assessing changes in the soil mycobiome. It showed that biofumigation, mainly with a forecrop of marigold (Tagetes patula L.) (R3), caused an improvement in soil physicochemical properties (bulk density and humus) and the highest increase in the abundance of operational taxonomic units (OTUs) of the Fungi kingdom, which was similar to that of agriculturally undegraded soils, and amounted to 54.37%. In this variant of the experiment, the most OTUs were identified at the phylum level, for Ascomycota (39.82%) and Mortierellomycota beneficial fungi (7.73%). There were no such dependencies in the soils replanted with forecrops of oilseed radish (Raphanus sativus var. oleifera) and white mustard (Sinapis alba). Biofumigation with marigold and oil radish contributed to a reduction in the genus Fusarium, which contains several significant plant-pathogenic species. The percentages of operational taxonomic units (OTUs) of Fusarium spp. decreased from 1.57% to 0.17% and 0.47%, respectively. [ABSTRACT FROM AUTHOR]

Marigold (Tagetes erecta) has a capitulum with two floret types: sterile ray florets and fertile disc florets. This distinction makes marigold a valuable model for studying floral organ development in Asteraceae, where MADS-box transcription factors play crucial roles. Here, 65 MADS-box genes were identified in the marigold genome, distributed across all 12 chromosomes. These genes were classified into type I (13 genes) and type II (52 genes) according to phylogenetic relationships. The gene structure of type I was simpler than that of type II, with fewer conserved motifs. Type I was further divided into three subclasses, Mα (8 genes), Mβ (2 genes), and Mγ (3 genes), while type II was divided into two groups: MIKCC (50 genes) and MIKC* (2 genes), with MIKCC comprising 13 subfamilies. Many type II MADS-box genes had evolutionarily conserved functions in marigold. Expression analysis of type II genes across different organs revealed organ-specific patterns, identifying 34 genes related to flower organ development. Given the distinct characteristics of the two floret types, four genes were specifically expressed only in the petals of one floret type, while twenty genes were expressed in the stamens of disc florets. These genes might have been related to the formation of different floret types. Our research provided a comprehensive and systematic analysis of the marigold MADS-box genes and laid the foundation for further studies on the roles of MADS-box genes in floral organ development in Asteraceae. [ABSTRACT FROM AUTHOR]

Fermentation experiments were conducted with a top-fermenting brewing yeast strain and wort enriched with three varieties of quinoa (with white, red and black grain color), as well as edible flowers (marigold and dandelion) to study their influence on the antioxidant properties of ale beer. Plant additives led to a slight increase in pH and a lower alcohol content of the beer. By itself, quinoa did not improve the functional properties of the beverage, but the combination of black quinoa and edible flowers resulted in an increased content of polyphenols in beer; marigold showed a 48.7 % increase in this parameter compared to the control, and dandelion - 51.2 %. The content of flavonoids in beer was not affected by the addition of plant additives to the wort. A slight increase in anthocyanins (0.7 - 4.4 %) was reported for black quinoa beer as well as black quinoa with flowers. A significant increase in antioxidant activity was reported for the variants with flowers (54 % for marigold and 56.6 % for dandelion). The increased antioxidant capacity combined with the reduced alcohol content of the beers with black quinoa and flowers suggests that a beverage with improved functional properties was obtained. Black quinoa, combined with edible flowers, was more favorable for increasing the antioxidant capacity of beer than other pseudo-cereals (amaranth and chia). [ABSTRACT FROM AUTHOR]

The article presents research on the use of Tagetes species hydrosols to combat fungi that affect strawberry plants. It was found that T. parryi hydrosol was effective in inhibiting the growth of B. cinerea both in vitro and in vivo. The study suggests that T. parryi hydrosol could be used as a preventive treatment against B. cinerea in strawberry plants. Additionally, the identification of chemical compounds in the hydrosols using a gas chromatograph is mentioned. [Extracted from the article]

Field studies on the "Development and evaluation of different management schedules against fruit borer, Helicoverpa armigera infesting tomato crop" were carried at farmer's field, village Surajgarh, Jhajjar, Haryana. The observations on infestation of fruit borer in tomato fruits at the time of each picking, %reduction of the fruit borer infestation, total marketable yield in different schedules over control and benefit cost ratio were calculated. The maximum larval reduction over control was observed in S6 i.e. spray of fenvalerate 20 EC @ 188 ml/ha (79.91%) followed by spray of nimbecidine 300 ppm @ 2.5 l/ha (79.04). On number basis, highest fruit damage reduction over control was observed in S6 (50.23%) followed by S5 (43.83%) and lowest (21.72%) in S4 i.e. African yellow marigold + yellow sticky trap + Trichogramma chilonis Ishi @ 50000 parasitised eggs/ha (2 releases at 4 days interval). On weight basis, %fruit damage reduction over control was maximum in S6 (57.47%) followed by S5 (53.10%) and minimum in S4 (25.06%). The highest benefit cost ratio (22.81) was recorded in S6 due to lowest cost of plant protection (Rs.3628.34/ha) and highest increased yield over control (72 q/ha). Although schedule S4 was next to S6 in term of total cost of plant protection but showed lowest benefit cost ratio (3.46) due to minimum increased yield over control (20 q/ha) among all the management schedules. Benefit cost ratio in remaining schedules were 8.97, 5.40, 3.71 and 3.58 in S5, S1, S2 and S3, respectively. [ABSTRACT FROM AUTHOR]

To support growth, short-cycle horticultural crops require readily available nutrients. However, this often leads to nutrient leaching. Implementing best management practices in production decisions like incorporating fertilizer retaining amendments to substrates or modifying fertilization programs can mitigate nutrient losses to the environment and associated costs. This study examined using an activated aluminum (AA) material as a substrate amendment to retain phosphorus (P) within containers while also assessing methods to reduce P fertilization in Tagetes production over a six-week production cycle. A commercial peat moss substrate (PL) pre-loaded with nutrients was amended with AA, enabling comparisons between substrates with and without AA. Enhanced fertilizer practices involved supplementing the initial nutrients by applying a weekly fertigation solution including nitrogen and potassium over the six weeks, but P for either 0, 2, 4, or 6 weeks. The incorporation of AA significantly reduced P leaching losses by 89.5–97.7%, compared to the PL substrates receiving P the entire six weeks. Regardless of substrate or fertilizer management, all Tagetes had equivalent sizes (growth index) and aboveground biomass. The results indicate that amending substrates with AA and/or reducing additional P inputs are effective strategies to minimize P leaching without compromising Tagetes quality. [ABSTRACT FROM AUTHOR]

Enhancing the quality of eggs by using natural food sources has become a very important topic in the last decade. The objective of this study was to determine the influence of natural (marigold and paprika extracts) pigments on the shelf life of eggs from laying hens. This research was carried out for a 6-week period on 168 Lohmann Brown laying hens (45 weeks age) divided into four groups (C, E1, E2 and E3) to assess the performances, external and internal egg quality parameters, egg yolk color, and antioxidant profile. The control group (C) was fed a standard diet (16.39% PB, 2750 kcal EM/kg compound feed) and the experimental diets were supplemented with 0.07% marigold extract (E1), 0.07% paprika extract (E2), and a mixture containing 0.07% of both extracts (E3). In summary, the study demonstrated that adding natural pigments from marigold and paprika extract with highly antioxidant lipid capacity into the diets of laying hens improved egg quality when eggs were stored at 28 days, under both storage temperature conditions (4 °C and 20 °C). [ABSTRACT FROM AUTHOR]

[Display omitted] Marigold analogues micro flowers of tungsten oxide (WO 3) have been grown in thin film form through simple and cost-effective solution chemistry approach on stainless steel substrate. Aqueous precursor involving WO 4 - 2 ions agglomerated as self-sacrificing template growing initially into the nano-petal, followed by self-assembly; leading to marigold analogues micro flower surface architecture. This enthralling morphology motivated us not only to fabricate supercapacitive electrode but also to design complete solid-state supercapacitor devices in dual configurations: flexible pouch cell and coin cell. Interestingly, both devices even in symmetric configuration yields remarkable potential window of 1.82 V when sandwiched by gel inclusive of Li+ ions dispersed in non-conducting polyvinyl alcohol matrix. Solid-state flexible pouch cell and coin cell delivered specific capacitances of 103.98 ± 3.59 and 30.09 ± 1.03 F/g respectively at a scan rate of 5 mV/s. Assembled electrode, coin-cell and flexible pouch-cells have been well assessed in-depth through specific capacitances using cyclic voltammetry and galvanostatic charge discharge, diffusive and capacitive contributions, mechanical bending tests, electrochemical active surface area, and electrochemical impedance analysis. Practical applicability has been demonstrated for designed flexible pouch cell to run small fan and light emitting diode panel whereas coin cell to run light emitting diode panel. [ABSTRACT FROM AUTHOR]

Globally, African marigold (MG) derived essential oils (EOs) have attained an immense economic pertinence in the flavor, fragrance, food, medicinal, and floricultural industries, which necessitate boosting its production on a commercial scale. Therefore, we aimed to assess varying levels (0, 50, 100, 150, and 200 mg L-1) of exogenously applied melatonin (MT) as a growth hormone to trigger growth, flower yield, and EOs of MG. The MT was applied as a foliar spray after thirty days of transplantation of MG plants and repeated thrice at fifteen-day intervals. The results depicted that exogenous MT (150 mg L-1) recorded the maximum plant height and leaves number along with fresh and dry weights of leaves and roots. The same treatment exhibited 66%, 64%, and 18% higher flower fresh and dry weights and flower yield respectively, than the control. Additionally, MT remained effective in reducing days taken to bud emergence and flowering, while flower retention duration increased by 11 days. Following the trend of vegetative growth traits, foliar-applied MT (150 mg L-1) remained unmatched in terms of physiological attributes (transpiration rate, stomatal conductance, chlorophyll a, and b contents) of MG. Moreover, for EOs extracted from fresh and dry flowers and leaves, this treatment remained effective by producing 77%, 73%, 53%, and 85% higher content, respectively than the control. Finally, the chemical profiling analyses detected eighty-seven chemical constituents (Caryophyllene oxide was the most dominant compound, and cis-Z-alpha-Bisabolene epoxide followed it) in MG flowers. In terms of the retention time of different chemical compounds in MG flowers, Calarene epoxide had the highest retention time of 19.75 minutes among major compounds. Based on these results, 150 mg L-1 dose of MT may be recommended to growers for boosting MG growth, floral yield and EOs content sustainably. [ABSTRACT FROM AUTHOR]

The current research was conducted to explore, for the first time, Tagetes erecta L. (family Asteraceae) fruits from northwest Iran in terms of the chemical composition of essential and fixed oils, their cytotoxic activities, and the inhibitory effect of essential oil on the PI3K/AKT/mTOR signaling pathway. The volatile oil was obtained through hydrodistillation (Clevenger apparatus). According to gas chromatography–mass spectrometry analysis, the essential oil was rich in cyclic monoterpenoids, 2-isopropyl-5-methyl-3-cyclohexen-1-one (19.99%), d-limonene (12.75%), terpinolene (11.64%) and also the saturated fatty acid palmitic acid (19.09%). Furthermore, the seeds of T. erecta were extracted using hexane by the maceration method. The analysis of fatty acid profile of the fixed oil by gas chromatography-flame ionization detector (GC-FID) demonstrated that the most predominant fatty acids in fixed oil were linoleic acid (59.53%), palmitic acid (13.70%), stearic acid (10.20%), and oleic acid (9.20%). The cytotoxic activity of essential oil, crude oil, and fraction A (obtained from fixed oil) were evaluated by using the MTT assay on MCF7 (human breast cancer cell line), PC3 (human prostate cancer cell line), and U87MG (human glioblastoma cell line). Finally, the effect of essential oil on inhibiting the PI3K/Akt/mTOR signaling pathway was evaluated using real-time PCR. The essential oil exhibited vigorous cytotoxic activity on the U87MG cell line, with an IC50 value of 32.65 μg/mL. Interestingly, the essential oil significantly inhibited the PI3K/AKT/mTOR cascade compared to the non-treated group. Our results suggest that the essential oil holds promise as an anticancer agent for glioblastoma cell lines. To the best of our knowledge, this study is the first to report on the profile of the essential oil of T. erecta fruits and its implications for targeting the PI3K/AKT/mTOR signaling pathway. [ABSTRACT FROM AUTHOR]

Lutein is widely used in medicine, health care, and food processing due to its antioxidant effects; however, it is difficult for the traditional extraction of lutein using marigolds to meet the increasing market demand for lutein. To achieve high-efficiency lutein production, we investigated the effects of different conditions on the biomass accumulation and lutein yield of Chlorella vulgaris. The optimized cultivation conditions include mixotrophic cultivation using sodium nitrate as a nitrogen source, maintaining a total-organic-carbon-to-total-nitrogen ratio of 12:1, a total-nitrogen-to-total-phosphorus ratio of 10:1, and lighting duration of 24 h. The results of the study indicated that under these specific conditions, Chlorella vulgaris attained a final biomass concentration, biomass productivity, and growth yield of 6.08 g·L−1, 1.00 g·L−1·d−1, and 1.67 g biomass/g TOC, respectively. Additionally, the concentrations of total chlorophyll, carotenoid, lutein, and protein reached 139.20 mg·L−1, 31.87 mg·L−1, 15.02 mg·L−1, and 2.17 g·L−1, respectively, and the content of lutein reached 2.47 mg·g−1. This study supplies a theoretical basis for the industrial application of lutein production using Chlorella vulgaris. [ABSTRACT FROM AUTHOR]

Using desi-cow waste products like Jeevamrit under natural farming is widespread among farmers for improving soil biology and productivity. Jeevamrit enhances soil chemical and microbiological properties without needing a large quantity of farmyard manure (FYM) as a sustainable farming practice with a reduced carbon footprint. Despite its traditional use, Jeevamrit faces criticism due to a lack of scientific evidence. This study investigated the comparative effect of Jeevamrit and chemical fertilizers on the growth and yield of marigold cv. Siracole. The experiment employed a randomized block design (RBD) with three replications. The mother block of marigolds was raised for both the summer and winter seasons. From this mother block, three harvesting flushes were taken and propagated from cuttings. The rooted cuttings were planted at monthly intervals and evaluated for flowering parameters and compared to those treated with RDF (30:20:20 N, P, and K g/m2). Soil supplied with Jeevamrit showed enhanced bacteria (26.33%), fungi (18.92%), and actinomycetes (31.21%) populations compared to the recommended dose of fertilizers (RDF) (i.e., N–P–K @ 30:20:20 g m−2). Jeevamrit-treated plants have a more marketable flower yield per square meter (3.98%) and a longer shelf life (9.93%) compared to RDF. The study concludes that Jeevamrit @ 2 liters m−2 is a sustainable and effective alternative to traditional fertilizers for enhancing marigold production in the mid-hills of Himachal Pradesh, where natural farming is already accepted. [ABSTRACT FROM AUTHOR]

The article investigates the mitigation of copper (Cu) toxicity with copper tolerant plant growth promoting rhizobacteria (PGPR) strains in marigold plant growth. The study discusses the in vitro testing of Cu-tolerant Providentia, Paenibacillus and Lysinibacillus PGPR strains and their effect on Cu accumulation by marigold. It measures plant growth promoting activities including production of siderophores, indole acetic acid, hydrogen cyanide and phosphate solubilization.

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Ultrasound-assisted extraction using response surface methodology was employed to extract marigold flower, resulting in a marigold flower extract (MFE) with elevated levels of total phenolic compounds (TPCs), total flavonoid content (TFC), total carotenoid content (TCC), and antioxidant activity, as assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays, under conditions of 40 °C temperature, 15 min extraction time, and 68% ethanol concentration. The MFE was subsequently encapsulated using spray drying with 45% maltodextrin (MD) (MFE–MD; 1:1, 1:2) and 20% gum arabic (GA) (MFE–GA; 1:2, 1:3). The MD (1:2 ratio) sample showed the highest encapsulation yield, while the 45% MD (1:1 ratio) sample exhibited the highest encapsulation efficiency (p ≤ 0.05). Samples containing 45% MD (1:1 ratio) and 20% GA (1:2 ratio) had the highest moisture content, with the 45% MD (1:1 ratio) sample showing the lowest water activity (p > 0.05). These samples also displayed higher L* and a* values compared to the 20% GA samples, which had increased b* values (p ≤ 0.05). Micrographs of the 20% GA (1:3 ratio) and 45% MD (1:2 ratio) samples revealed spherical shapes with smooth surfaces. The 20% GA (1:2 ratio) microcapsules exhibited the highest total phenolic content (TPC) among the samples (p ≤ 0.05). Thus, ultrasound-enhanced extraction combined with response surface methodology proved effective in producing functional food ingredients from plants. [ABSTRACT FROM AUTHOR]

Despite their high specific capacitances, metal oxide-based electrodes still do not meet the commercial standard for cycling stability owing to their inherent poor electronic conductivity and morphological structural changes during charging and discharging. A superior specific capacitance, accompanied with long-term cycling durability, is pivotal for supercapacitor application. In this research article, different precursor volume ratios of copper–nickel solution were used for the construction of binder-free 3D marigold flower-like copper–nickel oxide (3D-MCuNi oxide) electrodes via a hydrothermal method. XPS, EDAX, and TEM studies reveal that the increased amount of O-vacancies in the marigold flowers of the copper–nickel oxide composite is caused by a significant number of imperfections in the structure and might enhance their electrical conductivity. The marigold flower-like morphological structure exhibits electrode–electrolyte ions with significantly high hydrophilicity and better electrochemical diffusion performance. The optimized volume ratio of Cu : Ni = 1 : 1 for 3D-MCuNi oxide composite nanomaterials demonstrates an excellent specific capacitance of 2387.15 F g−1 at a current density of 0.6 mA cm−2 and 93% cycling capacitive retention performance up to 10 000 cycles. Additionally, a flexible hybrid supercapacitor device (HSD) was assembled using reduced graphene oxide (rGO) and 3D-MCuNi oxide as the negative and positive electrode, respectively, which revealed excellent electrochemical charge storage performance with a high energy density of 120.9 W h kg−1, power density of 34.82 kW kg−1, and superior cycling stability of 87% retention. Thus, the 3D-MCuNi oxide composite potential materials are more attractive for charge storage supercapacitor applications. [ABSTRACT FROM AUTHOR]

Botanicals from Marigold, Chrysanthemum, Basil, Rosemary and other medicinal plants have been analysed as potential replacements of chemical pesticides as they are effective and non-hazardous to the environment. The bioactive components thiophene is present in large amount in Tagetes sp. which have a wide range of biocidal properties. In this study the crude extracts of different parts of Tagetes erecta and Tagetes patula were obtained by Soxhlet extraction method. The leaf extracts were subjected to GC–MS to identify their components and to confirm the presence of thiophenes. Two major disastrous crop pests namely, Spodoptera litura and Corcyra cephalonica belongs to Order Lepidoptera, Family Noctuidae and Pyralidae were used for the experimental study. The effect of bioactive extract was tested by direct and indirect methods for their larvicidal effect. The S. litura larvae was allowed to feed on castor leaves coated with spraying different concentrations of methanolic sample extracts as indirect method. In the direct spraying method, the S. litura and C. cephalonica larvae were sprayed with low and high concentrations of crude sample extracts of both T. patula and T. erecta. Significant decrease in larval activity and survival rate within 24 h to 48 h were recorded for both the methods. The trials were performed using variousvolumes from 10 to 1000 µl to check larvicidal activity. The roots and stem extracts of T. erecta for 25 µl kill 100% larvae within 24 h whereas 50 µl of T. patula flowers, roots and stem extract effectively kill only 50% of the larvae in 48 h. A mini nursery evaluation were conducted by direct spraying method on larvae growing on jowar saplings, which displayed 100% mortality of larvae within 24 h for 25 µl of T. erecta leaf and flower extracts and T. patula flower extract. From these trials, it can be inferred that the extracts of T. patula and T. erecta provided effective outcomes as larvicides of S. litura and C. cephalonica. [ABSTRACT FROM AUTHOR]

The essential oil (EO) of Tagetes filifolia Lag. was obtained from dried plant material through Clevenger-type steam distillation and analyzed using gas chromatography–mass spectrometry (GC/MS), a gas chromatography–flame ionization detector (GC/FID) and enantioselective gas chromatography. The results showed 50 compounds (93.33%) with a predominance of oxygenated monoterpenes. The main components were trans-anethole (55.57 ± 9.83%), tridecene <1-> (8.66 ± 0.01), methyl chavicol (5.81 ± 0.85%) and Neophytadiene (3.45 ± 0.88) Enantioselective analysis revealed linalool and ionone as enantiomers. The identification of secondary metabolites from the ethyl acetate extract obtained by maceration was performed by GC-MS, NMR and by a literature comparison, determining the presence of mostly trans-anethole and a mixture of two triterpenes, fernenol and lupeol. [ABSTRACT FROM AUTHOR]

The cultivation of ornamental horticultural crops under salinity stress has been a challenge for growers all over the world. In this study, an attempt was made for pot cultivation of Marigold (Tagetes erecta L. var. Pusa Basanti Gainda) in salt-stressed (SS) soil (150 mM) with the combined use of mushroom compost leachate (CL) and foliar application of titanium dioxide nanoparticles (TiO2-NPs). For this purpose, a total of six pot treatments, i.e., borewell water (BW; control), T1 (BW with SS), T2 (BW with SS and TiO2-NPs), T3 (CL supplemented), T4 (CL with SS), and T5 (CL with SS and TiO2-NPs) were conducted in triplicate. The results of this study showed that CL supplementation significantly (p < 0.05) improved the physicochemical i.e., pH (14.5%), electrical conductivity (32.9%), total nitrogen (27.4%), total phosphorus (247.6%)), and nutrient (organic matter: 119.6%) profiles of soil which later helped in higher growth (30–35%) and yield (5.4–40.7%) of T. erecta. In CL-based treatments, the biochemical constituents were significantly (p < 0.05) higher than those in BW-irrigated ones. Also, the levels of selected stress defense enzymes were significantly increased under SS treatment but reduced under TiO2-NP application. Overall, it was observed that the combined application of CL and TiO2-NPs (T5 treatment) was the most helpful treatment for enhanced germination, growth, yield, biochemical parameters, and better plant enzymatic activities to cope with saline stress. This study provides a mechanistic understanding of T. erecta plants under saline stress which is crucial for the development of targeted interventions aimed at improving plant tolerance to saline conditions. [ABSTRACT FROM AUTHOR]

In the current study, the Cu phytoremediation ability of two ornamental plants, Chrysanthemum indicum L. and Tagetes erecta L., was tracked concerning the growth and physiological responses. Plants were subjected to varying concentrations of Cu (0, 100, 200, and 400 mg/kg) under the pot experiment for 8 weeks. The results showed that the measured growth and physiological characteristics declined in T. erecta shoots and roots at all tested treatments compared with the control. However, in C. indicum at 100 mg/kg, shoot biomass, shoot total soluble protein, and leaves number remained equal to that of the control and then reduced by rising Cu concentrations, compared with the control. Also, results indicated that in C. indicum, after 56 days of exposure to Cu, the chlorophyll pigments content markedly increased and reached a maximum level at 100 mg/kg dose and gradually declined with enhancing Cu concentrations, compared with the control. Other measured growth and physiological parameters decreased in both tissues of C. indicum in response to Cu usage in the growth medium. The carotenoid content of T. erecta decreased in all studied Cu levels in comparison to the control, but in C. indicum remained unaffected up to 200 mg/kg Cu in comparison to the control and then enhanced with increasing Cu level. The augmentation of antioxidant enzyme activity in two species, especially in roots, reflected the incident of Cu stress as demonstrated by elevated MDA and ion leakage levels. Data concerning copper accumulation in tissues, TF, and BAF showed T. erecta is a weak Cu accumulator and seems not to be an appropriate candidate for Cu phytoremediation. However, the Cu content in shoots and roots of C. indicum increased significantly with an increment in applied Cu level. Also, C. indicum accumulated higher Cu concentrations in the roots than in shoots and exhibited TF < 1, 0.1 < BAF root < 1, and can be considered as a Cu excluder by the phytostabilization mechanism. [ABSTRACT FROM AUTHOR]

The work is aimed to evaluate the blood pressure reducing effect of constituents from methanol extract and associated constituents of Tagetes patula flowers in normotensive and L-NAME induced hypertensive rats. The HPLC analysis of methanol extract of Tagetes patula flowers (JFM) resulted in the quantitative identification and percent comparison of four phenolic constituents, protocatechuic acid (PA), methyl protocatechuate (MPA), patulitrin (TRIN) and patuletin (PAT). All the extracts, fractions and compounds examined showed significant blood pressure lowering activity. Patulitrin (TRIN) which has emerged as the major constituent (15.33%) of T. patula flowers showed significant 30% and 68% fall in blood pressure in normotensive and L-NAME induced hypertensive rats respectively. The patuletin (PAT), which is an aglycone of TRIN displayed high percentage (84%) of antihypertensive activity. Further, comprehensive and advanced studies on these constituents may result in preparation of an effective blood pressure lowering medicine with active precious rare flavonoids, patuletin and patulitrin. [ABSTRACT FROM AUTHOR]

Background: Flowers are not only for their ornamental, aesthetic purpose but it is a great nutritious to human diet and health. These are rich source of pigments and possess antibacterial and antioxidant properties. Few flowers are used traditionally in diet and there is huge scope to use edible flowers in nutraceutical, pharmaceutical and pigment industry. But very less research has been done till date for finding proximate constituent, antioxidant activity and mineral profiling of edible annual flowers. The present work investigated the proximate constituent analysis and antioxidant activity of few annual flowers. Methods: In this laboratory analysis, different annual flowers were used for the experiment. They were Celosia cristata var. Deep Armor Purple, Celosia cristata var. Twisted Orange, Celosia cristata var. Pink, Celosia argentea var. Cristata Yellow, Celosia argentea var. Plumosa, Celosia cristata var. Bombay Pink, Gomphrena globosa, Gallardia pulchella, Tagetes erecta, Tagetes patula and Calendula officinalis. Proximate constituent analysis and antioxidant activity were performed using Association of Official Analytical Chemist (A.O.A.C) methods. Result: Among the different annual flowers, the maximum ash content (15.58%) the crude protein content (13.45%) and the crude fibre content (13.39%) were found in Gomphrena globosa flowers, whereas the minimum ash content (7.24%), the minimum crude protein (6.51%) and the minimum crude fibre content (6.63%) were reported in Calendula officinalis flowers. On the other hand, the antioxidant activity was found to be superior in the flowers of Tagetes patula (60.34%) followed by Tagetes erecta (57.99%) and the minimum antioxidant activity (43.89%) was found in flowers of Celosia cristata var. Twisted Orange. [ABSTRACT FROM AUTHOR]

• Tagetes erecta L. components (ray, disc florets, and oil) reduces the risk of ulcers. • Higher pH, reduced acidity, and lower ulcer index confirm herb's ulcer-protective effects. • Hydro-ethanolic extract showed significant dose dependent anti-ulcer potential. • Single oil dose reduces acute ulcers by 94 % and chronic ulcers by 96.93 %. • Prepared leaf oil surpasses herbal extract for superior ulcer protection. The Tagetes erecta Linn (family: Asteraceae) herb possesses medicinal and antioxidants value. The herb is traditionally used to treat various ailments, including ulcers. In order to investigate antiulcer activity of T. erecta herb , experimental study was performed between ray and disc florets extracted with 30 parts of water and 70 parts of ethanol solvents using Soxhlet apparatus and leaf's essential oil extracted in Clevenger apparatus by steam distillation method. Two doses of the hydroethanolic extract (400 and 800 mg/kg, Per os) and single dose of essential oil (10 ml/Kg, Per os) were administered for five consecutive days. Rat's pyloric ligation and aspirin (200 mg/kg, Per os) were used to induce acute and chronic gastric ulcers respectively. Antiulcer activity of both the herb's parts was compared on gastric juice parameters (pH, free acidity, total acidity) ulcer-index and ulcer percent protection. The p value of less than 0.05 was considered significant in statistical analysis. Elevation in pH, reduction in free acidity, total acidity and ulcer-index in both the ulcer models confirm that the test drug showed the ulcer-protective activity in rats. Dose-dependent effects were observed in the antiulcer activity of the hydroethanolic extract. However, the leaf's essential oil showed 94 % and 96.93 % ulcer protection in the pyloric ligation and aspirin-induced gastric ulcer models respectively. The hydroethanolic extract and the essential oil may be capable in part, regulating gastric acid secretion pathways or stimulating the cytoprotective secretion of bicarbonate, mucus and prostaglandins. Histological studies supported the observed antiulcer activity of the herb. The essential oil has more potent and significant anti-ulcer activity than hydro-ethanolic extract and respective standard drugs: ranitidine (50 mg/kg, Per os) & pantoprazole (20 mg/kg, Per os). The herb exhibits antiulcer activity, which could be due to the presence of lead phytochemicals: Quercetin-3-methyl ether, quercetin-7-methyl-ether & kaempferol flavonoids in the extract and limonene & terpinolene in leaf's essential oil and, thus, confirmed the traditional uses of T. erecta in the treatment of ulcers. The herb may cure acid pepsin disorders. [ABSTRACT FROM AUTHOR]

Tagetes is a genus consisting of several efficacious plant species known as marigold, which is native to Mexico and has spread worldwide due to its ability to adapt to different soil conditions. This genus is very popular among gardeners because it's easy to cultivate, adaptable, produces flowers all year round, has a free flowering habit, short duration, and the flower has an attractive color and shape. It has many flowers of varying colors such as red, orange, yellow, mixed red-orange or red-yellow, but the yellow and orange color are the most popular. The flowers contain many carotenoids, which act as antioxidants, antibacterial, anti-inflammatory, anti-carcinogen, nematicide, and cosmetics. The carotenoid can also cure fever, epileptic fits, astringent, scabies, liver complaints, stomachache, sore throat, and can be used as a natural moisturizer, and natural mosquito repellent Besides its use as a medicinal plant, Tagetes is also used as ornamental plants and edible plants. This manuscript's endeavour is to include some important investigations and studies about the general description, phytochemicals compounds and essential oils, medicinal uses, natural pesticides, and food and beverage uses of the marigolds. [ABSTRACT FROM AUTHOR]

Copyright of Scientific Journal of Agricultural Sciences (SJAS) is the property of Beni Suef University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Purpose: We analysed the possible synergistic activity among active extracts from Artemisia cina and Tagetes lucida combinations on Haemonchus contortus, a nematode parasitising sheep. Methods: The work was carried out in vitro on eggs and infective larvae (L3) of H. contortus. The results were analysed with SAS 9.1, applying the ANOVA and Tukey test, and the lethal concentration (LC) values LC50 and LC90 were determined with regression analysis, employing Proc Probit of SAS 9.1. Additionally, the lethal concentration (LC) was calculated with LC50 and LC90 to determine the synergistic effect. Results: The results demonstrated a high efficacy of the two plants studied on both nematode eggs and L3 larvae as well as of their combinations. The highest egg hatching inhibition was obtained with a 50/50 combination, and the best larvae mortality was obtained with 25% A. cina and 75% T. lucida at 10 mg/mL. Additionally, this combination showed a synergistic effect. Conclusion: The two plant species studied here can be applied as natural anthelmintic alternatives due to their high bioactive effect and synergistic response. [ABSTRACT FROM AUTHOR]

The aim of the present study is to: i) analyze the water absorption capacity of seeds of some Tagetes sp.; ii) compare the water absorption of Tagetes sp. and Lactuca sativa L. seeds in order to assess the potential of genus Tagetes as test object for laboratory screening studies for establishing allelopathic interference in plant communities. Tagetes erecta (local population from Germany), Tagetes patula (local population from Germany), and Tagetes patula variety “Usmivka” (Bulgarian variety) have been included in the study. Equivalence in the water absorption capacity (Ws%) of the seeds of studied Tagetes sp. and Lactuca sativa L. variety “Great Lakes” was found, with the maximum of water absorption occurring at the first hour and stopping by the fourth hour, regardless of the temperature range in which the imbibition takes place. The significant differences in the cumulative values of the average water absorption capacity of the Tagetes sp. seeds compared to Lactuca sativa L. variety “Great Lakes” (Ws% average ranged from +5.68 to +83.27%), indicate that the species of genus Tagetes can be successfully used in laboratory screening studies to establish allelopathic interference under in vitro conditions. Further research is needed to establish seed water absorption capacity (Ws%) of Tagetes sp. depending on the type of aqueous extracts used in allelopathic studies (cold or temperate), as well as of the concentrations applied. [ABSTRACT FROM AUTHOR]

• Chemical constituents and Pharmacological activities of Tagetes minuta have been classified according to the research in between 1992 and 2023. • Essential oils and thiophenes are major bioactive constituents present in Tagetes minuta. • Extracts and isolated compounds of Tagetes minuta showed antibacterial, antifungal, insecticidal, anti-diabetic, antioxidant, anti-inflammtory and anticancer activities. With a rich historical background, Tagetes minuta (L.) (T. minuta) belonging to the family Asteraceae has been traditionally employed to treat numerous ailments. Notably, its essential oil finds application in agrochemicals, perfumery and cosmetic industries. This review aims to provide up-to-date information on its phytochemical constituents and various pharmacological activities along with relevant published patents of this plant. This valuable data will be beneficial for researchers, as well as professionals in agrochemical, pharmaceutical, and cosmetics industries seeking up-to-date insights. The literature available on this plant was thoroughly analysed to extract information regarding its phytochemical composition, including essential oils, flavonoids, terpenoids, thiophenes, sterols and phenolics. Moreover, the pharmacological activities of T. minuta were critically examined encompassing antimicrobial, antiviral, antioxidant, antidiabetic, anticancer, antidepressant and insecticidal properties among others. Overall, this comprehensive and systematic review consolidates the existing knowledge on the phytochemistry and the pharmacological activities of T. minuta that underscore its immense potential as a valuable source of natural compounds with diverse therapeutic properties. Additional investigations are necessary to uncover the underlying mechanisms of its actions and conduct clinical trials to verify its effectiveness and safety in humans. This review provides valuable insight for researchers, pharmacologists, and herbal medicine practitioners, supporting evidence-based utilization of T. minuta in the field of medicine, pharmaceuticals, and natural product discovery. [ABSTRACT FROM AUTHOR]

The plant mediated synthesis of metallic nanoparticles is a fast-growing area of interest in the field of nanotechnology due to its eco-friendly approach. This study demonstrated the green synthesis of silver nanoparticles (AgNPs) using Tagetes minuta leaf extract. The AgNPs were characterised using UV-visible spectroscopy, transmission electron microscopy (TEM), energy dispersive X-ray (EDX) analysis and Fourier transform infrared (FTIR) spectroscopy. UV-visible spectrum of synthesised silver nanoparticles showed maximum peak at 442 nm. TEM revealed that the particles were spherical in shape and size ranging from 10 to 50 nm. The EDX spectrum confirmed the presence of silver metal. Preliminary antibacterial activity was determined using the agar well diffusion method, which showed growth inhibition against selected gram-positive and gram-negative bacteria. [ABSTRACT FROM AUTHOR]

Copyright of Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas is the property of Universidad de Santiago de Chile and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The current study placed an intense emphasis on the excess discharge of agrobased industrial effluent and the use of plant extract antimicrobials to inhibit the growth of pathogens in crop plants. An effluent (treated and untreated) from the marigold flower processing industry has been identified for the presence of volatile and semi-volatile organic compounds, and a total of 18 in treated effluent and 23 in untreated effluent were found using gas chromatography-mass spectrometry. A total of 13 classes were identified, which include carboxylic acid, phenols, esters, alkanes, alkenes, alcohols, cyanide, heterocyclic, flavonoids, aldehydes, polycyclic aromatic, cycloalkanes, and cycloalkenes. A principal component analysis with varimax rotation was applied to discern the abundance of identified compounds under each class. An in vitro antifungal bioassay was conducted using effluents at three different concentrations against plant pathogens (Alternaria alter nata, Sclerotium rolfsii, Rhizoctonia solani, Pythium aphanidermata, Fusarium oxysporum, and Colletotrichum gloeosporioides). The study proved that treated and untreated effluents clearly inhibited the growth of fungal pathogens by 10 to 32% and 37 to 92%, respectively. The findings suggest that marigold flower effluent can be a promising resource for developing new plant protection methods that are effective against pathogenic fungi. [ABSTRACT FROM AUTHOR]

Marigold flower is a traditionally used plant material topically applied on the skin due to its anti-inflammatory properties and antibacterial activity. This potential of action justifies the implementation of marigold extract in nanofiber scaffolds based on poly-vinylpyrrolidone/hydroxypropyl-β-cyclodextrin (PVP/HPβCD) and polycaprolactone/polyvinylpyrrolidone (PCL/PVP) obtained by electrospinning for wound treatment. Using SEM, the morphology of electrospun scaffolds showed a fiber diameter in the range of 298–527 nm, with a uniform and bead-free appearance. ATR-FTIR spectroscopy confirmed the presence of marigold extracts in nanofibrous scaffolds. The composition of the nanofibers can control the release; in the case of PVP/HPβCD, immediate release of 80% of chlorogenic acid (an analytical and functional marker for marigold extract) was achieved within 30 min, while in the case of PCL/PVP, the controlled release was achieved within 24 h (70% of chlorogenic acid). All systems showed weak antibacterial activity against skin and wound-infecting bacteria Staphylococcus aureus (MIC 100 mg/mL), and Pseudomonas aeruginosa (MIC 200 mg/mL) and yeasts Candida albicans (MIC 100 mg/mL). Analysis of the effect of different scaffold compositions of the obtained electrofibers showed that those based on PCL/PVP had better wound healing potential. The scratch was closed after 36 h, compared to the 48 h required for PVP/HPβCD. Overall, the study shows that scaffolds of PCL/PVP nanofibers loaded with classic marigold extract have the best potential as wound dressing materials because of their ability to selectively modulate inflammation (via inhibition of hyaluronidase enzyme) and supportive antimicrobial properties, thereby aiding in the early stages of wound healing and repair. [ABSTRACT FROM AUTHOR]

Essential oils (EOs) extracted from various medicinal plants offer a promising alternative to non-selective chemical substances commonly employed in conventional agriculture. Their chemical composition includes several classes of chemical compounds with beneficial properties, such as monoterpenes, sesquiterpenes, and phenylpropanoids, which can selectively control microbiological elements in soil and plants. The aim of the present study was to evaluate the essential oils and floral waters obtained from a new variety of marigold (Tagetes patula L., fam. Asteraceae, "Nanuk" variety) across various parameters, including biochemical characterization using GC-MS, antioxidant activity evaluated under three methods (DPPH, ABTS, FRAP), antimicrobial properties (for three G− bacteria: Perctobacterium carotovorum, Pseudomonas marginalis, Pseudomonas syringae and against three phytopathogenic fungi: Rhizoctonia solani, Fusarium oxysporum, Botrytis cinerea), and insecticidal activity. The results showed that when applied in high concentrations, marigold essential oil has a potential bactericidal effect on P. carotovorum, as well as a potential fungicidal effect on B. cinerea. [ABSTRACT FROM AUTHOR]

A rapid, eco-friendly, and simple method for the synthesis of long-lasting (2 years) silver nanoparticles (AgNPs) is reported using aqueous leaf and petal extracts of Tagetes erecta L. The particles were characterized using UV-Visible spectrophotometry and the analytical and crystallographic techniques of transmission electron microscopy (TEM). The longevity of the AgNPs was studied using UV-Vis and high-resolution TEM. The antibacterial activity of the particles against Erwinia amylovora was evaluated using the Kirby–Bauer disk diffusion method. The results were analyzed using ANOVA and Tukey's test (p ≤ 0.05). Both the leaf and petal extracts produced AgNPs, but the leaf extract (1 mL) was long-lasting and quasi-spherical (17.64 ± 8.87 nm), with an absorbance of UV-Vis λmax 433 and a crystalline structure (fcc, 111). Phenols, flavonoids, tannins, and terpenoids which are associated with -OH, C=O, and C=C were identified in the extracts and could act as reducing and stabilizing agents. The best antibacterial activity was obtained with a nanoparticle concentration of 50 mg AgNPs L−1. The main contribution of the present research is to present a sustainable method for producing nanoparticles which are stable for 2 years and with antibacterial activity against E. amylovora, one of most threatening pathogens to pear and apple productions. [ABSTRACT FROM AUTHOR]

During morphological evolution of gold nanoparticles, amino acids play a vital role in tuning shape, introducing chirality and inducing facet selective reactivity. Herein, we report the synthesis of unique marigold like structure (MGS) via growth reaction of methionine (Met) incubated positively charged anisotropic gold nanorod (GNR). Varying three important parameters such as growth time, concentration of Met and Au3+ reveals the combination of freshly generated small nucleated particles (fNPs) and GNR towards fabricating the unique MGS containing disk and ray floret parts. Strong interaction between Met and (111) plane of Au0 controls the orientation of (111) plane parallel to the direction of growth. This preferential interaction directs the assembly of gold nanostructures through Au (200) plane and results in merging of fNPs with concave GNR (cGNR) to fabricate the external arrangement of ray floret structure. The structural selectivity is attributed to the electron donating capacity of thioether functional group of Met(S) to Au+, generated prior to secondary nucleation. As confirmed by XPS and ζ‐potential analysis, the above interaction controls the Met concentration dependent inhibition of further Au+→Au0 reduction. The growth strategy of GNR has been further validated with a Met enriched peptide to produce disk and ray florets. [ABSTRACT FROM AUTHOR]

The edible flowers of marigold (Tagetes spp.) are cultivated for their aesthetic appeal and high utility as functional health food ingredients. Carotenoid and flavonoid contents in marigold petals highlight the importance of selecting the appropriate cultivar and its processing methods for their industrial applications. The comparative understanding of the effects of dry processing on functional components across different marigold cultivars is still lacking. Therefore, this study investigated functional compound changes in the dry processing effect on four marigold cultivars with distinct flower shapes (Durango, Inca) and colors (yellow, orange). The petals in hot air drying (HAD) with 30, 60, and 90 °C applications were analyzed for the measurement of their individual secondary metabolite contents, total phenolic and flavonoid contents, and antioxidant activities. In freeze drying (FD), the lutein content varied significantly based on flower color, exhibiting higher levels in cultivars with orange petals. Otherwise, the levels of quercetin derivatives displayed distinct differences based on varieties other than color, with Inca cultivars demonstrating higher levels of quercetin 7-O-glucoside (Q7G) than Durango cultivars. In HAD, the lutein levels show a tendency to increase above 60 °C regardless of the cultivar. The content of quercetin glycosides decreased, while the aglycone increased in HAD treatments, regardless of the temperatures. Correlation and PCA results highlighted the impact of phenol compounds on antioxidant activity. Overall, these findings underscore the significance of variety and color in determining the chemical composition and antioxidant properties of marigold flowers. [ABSTRACT FROM AUTHOR]

Nematodes represent a large percentage of soil biodiversity and are one of the most common organisms in soil ecosystems. Plant-parasitic nematode that causes significant crop losses all over the world. Meloidogyne incognita is one of the most common dangerous species to damage the root portion of plants. M. incognita-affected plants exhibit decreased cultivation, reduced development and yellowing of the leaves with dry tips and corners. Furthermore, there is decay of roots and gall forms. Zinc is a necessary component for plant growth and ZnO NPs are similarly essential in supporting disease defense mechanisms as they promote plant growth. When produced using environmentally friendly methods, these nanoparticles, which have been studied in a variety of fields, exhibit, anti-inflammatory, antimicrobial, and antioxidant, properties. This study analyzes the environmentally friendly synthesis of green synthesized ZnO NPs with an aqueous root extract from Tagetes erecta to examine the UV-Vis which showed an identifiable surface plasmon resonance (SPR) peak at 352 nm. Fouriertransform infrared spectroscopy (FTIR) was used to confirm the presence of frequency ranges at 3537.25, 2439.72,1373.94, 686.52, and 412.73cm-1 for the produced ZnONPS. The Debye-Scherrer equation was used to find the average crystalline size of the produced ZnoNPs, and the result was 29.34nm. These results indicate that most ZnO NPs aggregated into hexagonal in shape. The effects on second-stage juveniles (J2) larval mortality of the root-knot nematode Meloidogyne incognita, we carried out further in vitro analyses at various doses (100, 250, 500, 750, and 1000 μg/ml) and time intervals (24, 48, 72, 96, 120, and 144 hours). However, the maximum larval mortality rates (76.75% and 100% within 24 hours) were observed at doses of 750 and 1000 μg/ml. This study showed the effect of green synthesized ZnO NPs was found to influence the second-stage juveniles (J2) Meloidogyne incognita. [ABSTRACT FROM AUTHOR]

Marigold oleoresin is an oil-soluble natural colorant mainly extracted from marigold flowers. Xinjiang of China, India, and Zambia of Africa are the three main production areas of marigold flowers. Therefore, this study utilized ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS) technology, combined with Global Natural Products Social Molecular Networking (GNPS) and multivariate statistical analysis, for the qualitative and discriminant analysis of marigold oleoresin obtained from three different regions. Firstly, 83 compounds were identified in these marigold oleoresin samples. Furthermore, the results of a principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) indicated significant differences in the chemical compositions of the marigold oleoresin samples from different regions. Finally, 12, 23, and 38 differential metabolites were, respectively, identified by comparing the marigold oleoresin from Africa with Xinjiang, Africa with India, and Xinjiang with India. In summary, these results can be used to distinguish marigold oleoresin samples from different regions, laying a solid foundation for further quality control and providing a theoretical basis for assessing its safety and nutritional aspects. [ABSTRACT FROM AUTHOR]

A novel halotolerant actinobacterium, designated as RG38T, capable of producing black extracellular melanin pigment on SP2 agar, was isolated from the roots of Tagetes patula. Comparative analysis of the 16S rRNA gene sequence revealed the highest similarity to Streptomyces collinus NBRC 12759T (99.3%). Phylogenetic analysis showed that strain RG38T clustered within the genus Streptomyces forming a monophyletic cluster with its close relatives. The average nucleotide identity (ANI), digital DNA-- DNA hybridization (dDDH), and amino-acid identity (AAI) values between strain RG38T and related species within the genus Streptomyces were below the standard threshold for prokaryotic species delineation. The DNA G + C content of the strain RG38T was determined to be 73.3%. The genome size measured 7,150,598 bp comprising 17 contigs and encompassed 6,053 protein coding genes. AntiSMASH analysis of the whole genome revealed 35 putative biosynthetic gene clusters (BGCs) responsible for various secondary metabolites. Among these clusters, two gene clusters exhibited 100% similarity to the chromomycin A3, albaflavenone, and anthracimycin, respectively. These compounds were reported to possess significant anticancer and antibacterial activities. LC--MS-based analysis, coupled with further isolation studies, confirmed the production of chromomycins A2 (1), A3 (2), and their derivatives, along with their antibiotic activities. These findings underscore the potential of this novel strain as a novel resource for the discovery of diverse antimicrobial compounds. This study is the first to report an antimicrobial compound producing Streptomyces species isolated from medicinal plant T. patula. Based on a polyphasic study, the strain RG38T isolated from an unexplored habitat with a high potential for new natural products represents a novel species within the genus Streptomyces. Accordingly, we propose the name Streptomyces tagetis sp. nov. for this novel species, with the type strain is RG38T (=KCTC 49624T = BRC 15113T). [ABSTRACT FROM AUTHOR]

Tagetes imbricata, a species recently described from Argentina and Bolivia, is reported here for the first time from Peru based on herbaria collections and field observations available at biodiversity online repositories. This new record significantly expands the distribution area of this species to the north, covering a total of more than 2000 kilometers along the Andes Mountains. An illustration for T. imbricata is here provided for the first time, detailing morphological aspects. Two vernacular names, "kita-chick-chipa" and "chichijsa", are reported for this species. [ABSTRACT FROM AUTHOR]

Marigold is a plant native to the Mediterranean region, belonging to the Asteraceae family, and has been cultivated as a medicinal and ornamental plant all over the world for centuries. Due to its long flowering period, it is important to determine the appropriate harvest interval and date. This study was performed in the Research Area of Çukurova University in the 2019–2020 and 2020–2021 growing seasons, with an aim to investigate the influence of different harvest intervals (4-, 8-, and 12-day harvest intervals for the flowers) and harvest dates (8-day interval for the seeds) on the flower and seed yield and quality traits of marigold. The highest total flower yield (7519.8 kg/ha; harvest at 4-day intervals) and seed yield (595.6 kg/ha; 1st harvest) were obtained in the 1st growing season. The highest seed fixed oil ratio (12.86 %) was obtained in June, and the highest flower essential oil ratio (0.18 %) was obtained in May. Of the 38 components in the essential oil, α-Cadinol (22.59 %–30.10 %), δ-Cadinene (9.88 %–13.79 %), τ-Muurolol (6.58 %–8.20 %), γ-Cadinene (4.80 %–7.15 %), and γ-Muurolene (4.15 %–4.82 %) were determined as the main components. Six fatty acids were detected in the fixed oil and the main components were determined as α-calendic acid (22.07 %–49.41 %), linoleic acid (28.31 %–37.85 %), and oleic acid (7.18 %–30.06 %). The highest α-calendic acid ratios were detected in June and decreased as the harvests were delayed. Consequently, the application of harvesting at 4-day intervals for increased flower number and harvesting seeds early due to the high α-calendic acid rate can be considered as a feasible approach. [Display omitted] • At 32 °C, both the flower number and diameter values decreased rapidly. • The flower diameter values significantly affected the fresh flower yield values. • The heteromorphism ratio in the marigold seeds varied according to the harvest times. • Plants stressed by the effects of rising temperatures formed smaller capitula. • The α-calendic acid ratio tended to decrease with increasing temperatures. [ABSTRACT FROM AUTHOR]

French marigold (Tagetes patula L.) is an aromatic plant known for its repellent effects on pests. It is traditionally grown in fields near many vegetable crops, including potato (Solanum tuberosum L.). However, the effects of marigold essential oil (EO) on the physiology of neighboring crops have been neglected in research. The aim of this study was to establish, for the first time, a comprehensive phytohormone profile of potato plants exposed to French marigold EO for different time periods (4, 8 and 12 h). Endogenous levels of all major phytohormone groups, determined by HPLC–MS analysis, showed altered phytohormone responses of EO-exposed potato plants. The most affected were stress-related phytohormones: abscisic acid–glucose ester, 1-aminocyclopropane-1-carboxylic acid, and jasmonic acid. Increased levels of jasmonic acid, cytokinins, storage form of abscisic acid together with decreased levels of indole-3-acetic acid and ethylene precursor were observed. In most of the analyzed phytohormone groups similar response pattern was observed—an increase in levels after short time exposure (4 h), followed by a decrease to control values after prolonged exposure (8 h and 12 h). Expression levels of genes involved in biosynthesis and catabolism of stress-related phytohormones, obtained by de novo bioinformatic processing of data from cDNA microarray analysis, revealed that jasmonic acid biosynthetic pathway was the most affected, with the highest number of altered transcripts and with one of the genes from this pathway (12-oxophytodienoate reductase 1-like) exhibiting the highest expression rate. [ABSTRACT FROM AUTHOR]

Copyright of Scientific Journal of Agricultural Sciences (SJAS) is the property of Beni Suef University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Scientific Journal of Agricultural Sciences (SJAS) is the property of Beni Suef University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)