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The aim of this study was to investigate the effects of ionizing radiation (IR) on ciliogenesis of human glioblastoma cells. The glioblastoma cell lines M059K and M059J were exposed to X-ray or carbon ion at different doses, and the incidence and length of primary cilia that emerged were detected by immunofluorescence. A colony formation assay was used to investigate the influence of primary cilia interference by siRNA against IFT88 (siIFT88) on the cellular sensitivity to IR. The results showed that both M059K and M059J harbor primary cilia, with the incidence in M059K and M059J cells at (41.36±4.75)% and (8.07±1.58)%, respectively. The average length of cilia in both cells exceeded 3 μm, with M059J cells having lower frequency and shorter length of primary cilia, and exhibiting a prominently higher radiosensitivity compared to M059K cells. Conversely, X-ray irradiation significantly increased the incidence of primary cilia in M059K cells, and the rate of ciliogenesis reaching approximately 60% when exposed to 10 Gy. Notably, suppression of ciliogenesis by siIFT88 remarkably elevated the radiosensitivity of M059K cells. Moreover, IR-induced ciliogenesis showed dose-dependent and time-response characteristics. In summary, IR promotes ciliogenesis by increasing both number and length of primary cilia, and the obstruction of primary ciliogenesis enhances the radiosensitivity of glioblastoma cells.

Background: The laparoscopic approach has been proposed for treating hepatic cystic echinococcosis (HCE) and has already been used in clinical practice, mostly for non-radical operations. In this study, we aimed to evaluate the feasibility of total cystectomy of HCE under laparoscopy (LS). Results: A retrospective review of the medical records obtained from 22 patients diagnosed with HCE between June 2009 and June 2013 and treated with an LS approach was conducted in the First Affiliated Hospital of Xinjiang Medical University. A total of 15 patients underwent total cystectomy of HCE using LS. The average time of surgery was 174 min (160–210 min). Intraoperative bleeding was 103 mL (80–200 mL). The mean duration of hospitalization was 7 days (6–15 days). Seven patients were transferred to open surgery (OS). For these patients, the average duration of surgery was 177 min (150–230 min). Intraoperative bleeding was 237 mL (160–350 mL), and the mean duration of hospitalization was 10 days (8–15 days). The most frequent postoperative complications were hydrops in the surgical area (two cases in LS and three cases in OS), and temporary bile leakage (one patient in the LS group). Recurrence was not seen in any cases in either group with a follow-up of 6–12 months. Conclusions: Total cystectomy of HCE appears to be safe and effective in selected patients with unique, small-sized, superficially located cysts. To establish precise recommendations about the technique and its indications, prospective studies are necessary.

KRAS is one of the most mutated genes, driving alternations in metabolic pathways that include enhanced nutrient uptaking, increased glycolysis, elevated glutaminolysis, and heightened synthesis of fatty acids and nucleotides. However, the beyond mechanisms of KRAS-modulated cancer metabolisms remain incompletely understood. In this review, we aim to summarize current knowledge on KRAS-related metabolic alterations in cancer cells and explore the prevalence and significance of KRAS mutation in shaping the tumor microenvironment and influencing epigenetic modification via various molecular activities. Given that cancer cells rely on these metabolic changes to sustain cell growth and survival, targeting these processes may represent a promising therapeutic strategy for KRAS-driven cancers. [ABSTRACT FROM AUTHOR]

ABSTRACT Frame insertion and deletion are common inter-frame forgery in digital videos. In this paper, an efficient method based on quotients of correlation coefficients between local binary patterns (LBPs) coded frames is proposed. This method is composed of two parts: feature extraction and abnormal point detection. In the feature extraction, each frame of a video is coded by LBP. Then, quotients of correlation coefficients among sequential LBP-coded frames are calculated. In the abnormal point detection, insertion and deletion localization is achieved by using Tchebyshev inequality twice followed by abnormal points detection based on decision-thresholding. Experimental results show that our method has high detection accuracy and low computational complexity. Copyright © 2014 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]

Phosphatase and tensin homolog ( PTEN ) is one of the most important tumor suppressor genes. Although studies have shown the association between cancer and genetic polymorphisms of PTEN , the underlying molecular mechanisms of breast cancer (BC) chemosensitivity that results from PTEN polymorphism is still unclear. This study aims to investigate potential links between PTEN polymorphisms in cis-regulatory elements and BC chemosensitivity in the Chinese population. A total of 172 BC patients who received neoadjuvant chemotherapy were included in the study, including 104 chemosensitive cases and 68 chemoresistant cases. The results showed a significant association between the rs786204926 polymorphism and BC chemosensitivity. Logistic multivariate regression analysis showed that age, lymph node metastasis, and the rs786204926 genotype were risk factors for BC chemoresistance. The G allele of rs786204926 is more prone to increasing the risk of chemosensitivity in BC. Additionally, analysis using Alamut Visual showed a preference of the G allele of rs786204926 to produce a novel PTEN mutant with an insertion of 18 bases from intron 4. While the transcriptional level of PTEN remained similar in chemosensitivity and chemoresistant samples, its protein level changed significantly. Interestingly, there were significant differences in both transcription and protein levels of the novel PTEN mutant between the two groups. Furthermore, we found that the mutant was more susceptible to dephosphorylation compared with wildtype PTEN, leading to chemosensitivity through the PI3K-AKT signaling pathway. These findings indicate that novel PTEN mutants caused by polymorphisms in cis-regulatory elements may be involved in BC chemosensitivity., Competing Interests: None., (AJCR Copyright © 2023.)