1. Physicochemical and biological characterization of nanocomposites made of segmented polyurethanes and Cloisite 30B
- Author
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J San Román del Barrio, R. Solis-Correa, JI Moo-Espinosa, José M. Cervantes-Uc, Mar Fernández Gutiérrez, Juan V. Cauich-Rodríguez, R.F. Vargas-Coronado, P Quintana Owen, and MA Aguilar-Santamaría
- Subjects
Thermogravimetric analysis ,Materials science ,Cell Survival ,Polyurethanes ,Biomedical Engineering ,Mitosis ,Biocompatible Materials ,Nanocomposites ,Biomaterials ,Differential scanning calorimetry ,X-Ray Diffraction ,Tensile Strength ,Materials Testing ,Spectroscopy, Fourier Transform Infrared ,Polymer chemistry ,Humans ,Nanotechnology ,Thermal stability ,Lymphocytes ,Viability assay ,In situ polymerization ,Fourier transform infrared spectroscopy ,Cells, Cultured ,Nanocomposite ,Fibroblasts ,Bentonite ,Microscopy, Electron, Scanning ,Thermodynamics ,Glass transition ,Nuclear chemistry - Abstract
Nanocomposites were prepared with segmented polyurethanes and Cloisite 30B by either solution mixing or in situ polymerization and characterized by Fourier transform infrared spectroscopy, differential scanning calorimetry, thermogravimetric analysis and X-ray diffraction. Cytotoxicity and genotoxicity were assessed with lymphocytes while cell viability was measured by the methyl tetrazolium assay using fibroblasts. It was found that in situ polymerization rendered exfoliated nanocomposites with higher glass transition temperature, tensile modulus and thermal stability compared to nanocomposites obtained by solution mixing. The mitotic index of lymphocytes was significantly reduced at high clay concentrations (6 wt% and 10 wt%), while fibroblast viability improved in the presence of extract obtained after days 2 and 7.
- Published
- 2013
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