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2 results on '"M1 monocyte"'

1. Blood M2a monocyte polarization and increased formyl peptide receptor 1 expression are associated with progression from latent tuberculosis infection to active pulmonary tuberculosis disease

Author

Yung-Che Chen, Yu-Ping Chang, Chang-Chun Hsiao, Chao-Chien Wu, Yi-Hsi Wang, Tung-Ying Chao, Sum-Yee Leung, Wen-Feng Fang, Chiu-Ping Lee, Ting-Ya Wang, Po-Yuan Hsu, and Meng-Chih Lin

Subjects
Active tuberculosis disease, Latent tuberculosis infection, M1 monocyte, M2a polarization, Formyl peptide receptor1/2/3, Infectious and parasitic diseases, RC109-216
Abstract

Objectives: This study aims to explore the role of M2a polarization and formyl peptide receptor (FPR) regulation in the reactivation of Mycobacterium tuberculosis (Mtb) infection. Methods: M1/M2a monocyte percentage and FPR1/2/3 protein expression of blood immune cells were measured in 38 patients with sputum culture (+) active pulmonary TB disease, 18 subjects with latent TB infection (LTBI), and 28 noninfected healthy subjects (NIHS) using flow cytometry method. Results: M1 percentage was decreased in active TB versus either NIHS or LTBI group, while M2a percentage and M2a/M1 percentage ratio were increased. FPR1 expression on M1/M2a, FPR2 expression on M1, and FPR3 expression of M1 were all decreased in active TB versus LTBI group, while FPR1 over FPR2 expression ratio on NK T cell was increased in active TB versus either NIHS or LTBI group. In 11 patients with active TB disease, M1 percentage became normal again after anti-TB treatment. In vitro Mtb-specific antigen stimulation of monocytic THP-1 cells resulted in M2a polarization in association with increased FPR2 expression on M2a. Conclusions: Increased M2a and decreased M1 phenotypes of blood monocyte may serve as a marker for active TB disease, while decreased FPR1 on blood monocyte may indicate LTBI status.

Published
2020
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2. Blood M2a monocyte polarization and increased formyl peptide receptor 1 expression are associated with progression from latent tuberculosis infection to active pulmonary tuberculosis disease

Author

Sum-Yee Leung, Ting-Ya Wang, Tung-Ying Chao, Yi-Hsi Wang, C Lee, Wen-Feng Fang, Chao-Chien Wu, Chang-Chun Hsiao, Yung-Che Chen, Yu-Ping Chang, Po-Yuan Hsu, and Meng-Chih Lin

Subjects
0301 basic medicine, Microbiology (medical), Adult, Male, T cell, 030106 microbiology, Formyl peptide receptor1/2/3, Formyl peptide receptor 1, Monocytes, Sputum culture, lcsh:Infectious and parasitic diseases, Mycobacterium tuberculosis, 03 medical and health sciences, 0302 clinical medicine, Immune system, Latent Tuberculosis, medicine, Humans, Latent tuberculosis infection, lcsh:RC109-216, 030212 general & internal medicine, Tuberculosis, Pulmonary, Aged, Formyl peptide receptor, Latent tuberculosis, biology, medicine.diagnostic_test, business.industry, M2a polarization, Monocyte, Cell Polarity, Active tuberculosis disease, General Medicine, Middle Aged, medicine.disease, biology.organism_classification, bacterial infections and mycoses, M1 monocyte, Receptors, Formyl Peptide, Infectious Diseases, medicine.anatomical_structure, Immunology, Disease Progression, Female, business, Biomarkers
Abstract

Objectives This study aims to explore the role of M2a polarization and formyl peptide receptor (FPR) regulation in the reactivation of Mycobacterium tuberculosis (Mtb) infection. Methods M1/M2a monocyte percentage and FPR1/2/3 protein expression of blood immune cells were measured in 38 patients with sputum culture (+) active pulmonary TB disease, 18 subjects with latent TB infection (LTBI), and 28 noninfected healthy subjects (NIHS) using flow cytometry method. Results M1 percentage was decreased in active TB versus either NIHS or LTBI group, while M2a percentage and M2a/M1 percentage ratio were increased. FPR1 expression on M1/M2a, FPR2 expression on M1, and FPR3 expression of M1 were all decreased in active TB versus LTBI group, while FPR1 over FPR2 expression ratio on NK T cell was increased in active TB versus either NIHS or LTBI group. In 11 patients with active TB disease, M1 percentage became normal again after anti-TB treatment. In vitro Mtb-specific antigen stimulation of monocytic THP-1 cells resulted in M2a polarization in association with increased FPR2 expression on M2a. Conclusions Increased M2a and decreased M1 phenotypes of blood monocyte may serve as a marker for active TB disease, while decreased FPR1 on blood monocyte may indicate LTBI status.

Published
2020

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