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Morinda citrifolia L. (Mengkudu) leaf is not well-known for its benefits compared to M. citrifolia fruit. It can be considered a good source in healing disease and anti-cancer properties due to the high content of antioxidant enzymes. The objective of this study was to determine the antioxidative activities of M. citrifolia leaves extracted at different pH (pH 3 to pH 9) and temperatures (20°C to 80°C), based on four types of antioxidant enzymes test, namely catalase (CAT), peroxidase (POD), polyphenol oxidase (PPO) and superoxide dismutase (SOD). Another analytical test is the protein determination of M. citrifolia leaves using Bovine Serum Albumin as standard. All of the tests were conducted using spectroscopy methods. Catalase (CAT) activity was monitored by reduction of absorbance due to the decomposition of hydrogen peroxide and peroxidase (POD) activity was observed by an increment of absorbance caused by the oxidation of 4-methylcatechol by hydrogen peroxide. Polyphenol oxidase (PPO) was monitored by an increment of absorbance due to the oxidation of 4-methylcatechol and superoxide dismutase (SOD) activity was determined using the NBT-based method, which monitors the amount of enzyme causing 50% inhibition of photochemical reduction of NBT. Results indicated that CAT activity and POD activity were significantly highest (p0.05) was observed for PPO activity in pH treatment. For different temperature treatments, CAT activity was significantly highest (p

Non-communicable diseases such as diabetes, atherosclerosis, myocardial infarction, and hypertension have rapidly increased. The scenario is due to unhealthy eating habits and unhealthy foods with high fat and oil, and high sugar on the market. A snack bar is one of the most suitable products as ready-to-eat food. It would be an ideal healthy food due to consumer demand for fast-growing natural foods. The application of Gum Arabic to confectionaries (snack bars) in the food industry is still low. Gum Arabic produced by the stem and branches of Acacia seyal has an excellent functional value of dietary fibre and the potential as an effective source in the formulation of a high fibre GA snack bar. This study was conducted to evaluate the sensorial and physicochemical characteristics of GA snack bar formulation with different concentrations of Gum Arabic of 10%, 20%, and 30%. Sensory analysis was conducted by using an acceptance test which is a 5-point hedonic scale. The attribute consists of colour, appearance, hardness, crispness, taste, and overall acceptance. For physicochemical properties, the colour, texture (i.e., hardness and crispness), pH, total soluble solid, water activity, moisture, crude protein, crude fat, crude ash, crude fibre, carbohydrate, and calorie determination are included. However, the value of a*, b* and protein were significantly (p

Water lily (Nymphaea antares) is one of the most valuable aquatic ornamental plants which has bright potential in the floriculture industry. It may be useful as an urban ecosystem and as a source of medicinal compounds. Due to its potential to become a new value-added product in the food industry, water lily (N. antares) was investigated in this study. Therefore, the goal of this study was to determine the nutritional content and antioxidant activity in water lily leaves and petioles with different pH extraction buffers. Water lily extract was obtained using three different pH extraction buffers, Tris buffer at pH 6.8, pH 7.1 and pH 7.8. The heat capacity of the extract was analysed using differential scanning calorimetry (DSC) and different functional groups were identified using Fourier transform infrared spectroscopy (FTIR). Thermal denaturation of the leaves sample was detected at 81.84°C. The antioxidant enzymes activities including catalase (CAT), peroxidase (POX), polyphenol oxidase (PPO), and superoxide dismutase (SOD) were determined in the leaves and petioles of water lily. In leaves, PPO activity was found to be higher in samples with pH 7.1 of the extraction buffer while there were no significant differences for activities of CAT, POX and SOD in all samples. In petioles, PPO and POX activity were found to be higher in samples with pH 7.8 and 7.1 of extraction buffer, respectively. Thus, the study found that a pH range of 7 to 9 extraction buffers did not greatly affect most of the analysis performed.

Gluten is detrimental to people who suffer from gluten-related disorders. Recently, the upsurge in demand for gluten-free products can be traced not only from the population that suffered from gluten-related disorders but also people who prioritize healthy lifestyles and practising gluten-free diets. One of the most challenging tasks in the development of gluten-free products is their quality. The purpose of this review is to describe the application of gluten-free flours in common bakery products such as bread, cakes, and biscuits. This article does summarize some functional ingredients such as hydrocolloids, protein, and enzymes and also the applications of novel technological approaches including high-pressure treatment, sourdough fermentation and extrusion technology. Overall, different approaches utilized in the improvement of gluten-free bakery products will lead to various quality outcomes.

Spent coffee grounds (SCG) a by-product from coffee industries, coffee shops and domestic users contain large amounts of organic compounds which could be re-used as source antioxidants for foods or cosmetics. This project aims to study the antioxidant activity of three different spent ground coffee varieties (Robusta, Liberica and Arabica) extracted using ultrasonic-ethanol assisted extraction method utilizing 60% ethanol. The extracted samples were analysed using DPPH, FRAP, FTC and TBA, total phenolic content, total flavonoid content and also individual flavonoids to measure the quality and quantity of antioxidative activity in spent coffee. Robusta and Liberica SCGs exhibited similar activity (p>0.05) towards DPPH scavenging and ferric reducing reaction. Both showed 77.99±0.92 and 77.75±0.04%, respectively for DPPH, and 26.82±2.92 and 24.41±0.49 mg TE/g dry sample, respectively for FRAP. In FTC and TBA analyses, Robusta SCG was found to have the least activity with 61.07±2.43% and 2.9±0.14% respectively. Spent Arabica has similar inhibition percentage (p>0.05) as Liberica SCG in FTC (57.08±0.9 and 50.54±4.23% respectively), yet has different inhibition activity (p

This study aimed to determine the proximate compositions and functional properties of sweet potato flour from different varieties cultivated in Sabah, Malaysia, namely Jepun, Kairot and Kaladi. The results showed that the moisture content of all flour samples was below 14%. The fat and protein content of Jepun sweet potato variety were significantly different (p

Banana is a climacteric fruit that is available throughout the region of Malaysia. In this study, banana flour was produced from different varieties of unripe banana, namely Saba, Mas and Berangan and characterised in its chemical composition and functional properties. The results obtained showed that the chemical composition of the flour varied according to the variety. The ranged obtained were 3.71 – 4.53% crude protein, 10.32 – 10.72% moisture, 0.12 - 0.16% crude fat, 1.20 – 2.38% ashes, 0.68 – 1.19% crude fibre, 81.95 – 83.15% carbohydrate and 8.00 – 8.54% dietary fibre. The colour analysis found that significant differences were observed for the L*, a* and b* values of all varieties of banana flours. Flour from Saba cultivar was significantly different from other banana flours in its highest pH value (5.79), oil absorption capacity (1.08 g/g) as well as emulsion activity (17.36%) and had the smallest particles size. Flour obtained from Mas variety had the highest foaming capacity (10.85%) and the swelling capacity while Berangan cultivar had the highest water absorption capacity (1.10%). Pasting profiles of banana flours differed significantly for peak, final, breakdown and setback viscosities. It was ranged from 1511.17 - 3145.33, 4197.00 - 5213.67, 1419.67 - 2871.00 and 4401.00 - 5121.67 cP, respectively. Gelatinisation properties of all banana flours showed significant differences in peak temperatures, whereas no significant differences were observed for enthalpy (ΔH).

Clitoria ternatea, commonly known as Butterfly pea flower or Bunga telang, is a tropical flower and notable as one of the most vital sources of polyphenols with high antioxidant capacity. C. ternatea abundantly produces anthocyanin compounds benefiting natural blue colourants. To date, a blue colourant is the most difficult to obtain since the anthocyanins responsible for this colour is the least stable and easily degraded after extraction. Their stability commonly relies on several operational parameters, such as shifted pH value, high temperature, and light exposure. Apart from colour attributes, anthocyanins have also been identified as a source of functional molecules due to antioxidant activity and beneficial health effects such as anti-cancer, anti-obesity, antimicrobial and more. This study aimed to characterize the chemical constituents of the extractable polyphenols from ethanolic extract of C. ternatea quantitatively. The numerical data were evaluated using the Minitab version 18 statistical method. The chemical profiling, including total phenolic content (TPC), total flavonoid content (TFC), total monomeric anthocyanins (TMA) were determined using standard methods. The antioxidant activity was evaluated using DPPH and ABTS methods. The results showed that the ethanolic extract of C. ternatea had 35.7 mg QE/g of TFC, 102.4 mg GAE/g280nm and 28.8 mg GAE/g750nm of TPC and 2.7 CE/g and 2.9 ME/g of TMA. The total phenolic content of C. ternatea showed a good correlation with the antioxidant activity by the DPPH method when analysed using Pearson correlation and showed an increasing trend of antioxidant power with regards to TPC in both assays. In a nutshell, this study contributes to the knowledge of ethanolic extract of C. ternatea. Future research could consider exploring other extraction methods that could enhance the stability of polyphenols particularly the anthocyanins from degradation such as using surfactants.

This study was aimed to optimise the Alcalase® enzymatic hydrolysis extraction of Asiatic hard clam (AHC) (Meretrix meretrix) protein hydrolysate in terms of hydrolysis time, hydrolysis temperature, hydrolysis pH, and concentration of enzyme. Protein hydrolysate produced from AHC (M. meretrix) meat was used to determine the optimum hydrolysis conditions. Hydrolysis of AHC meat was optimised using the Central Composite Design Response Surface Methodology (RSM) (CCD). The relationship between four parameters such as temperature (45 – 65°C), enzyme to substrate concentration (1 – 2%), hydrolysis time (60 – 180 mins), and pH (7.5 – 9.5) to the degree of hydrolysis was investigated. The optimum conditions for enzymatic hydrolysis of AHC meat to achieve the maximum degree of hydrolysis (DH) were observed at 65°C, enzyme to substrate concentration of 1%, hydrolysis time of 60 mins, and pH 7.5. The enzymatic protein hydrolysis of AHC meat was predicted using a two factors interaction (2FI) model. Under these optimum conditions, DH's predicted value was 97.41%, which was close to the experimental value (97.89%). The freeze-dried protein hydrolysate powder was characterized concerning the proximate composition. Proximate analysis revealed that the AHC meat contains 7.92±1.76% of moisture, 2.23±0.89% of crude fat, 1.98±0.82 of ash, and 10.53±0.04% of crude protein. While the Asiatic hard clam protein hydrolysate (AHCPH) composed 9.12±0.02% of moisture, 0.80±0.29% of crude fat, and 27.76±0.10% of ash. The protein hydrolysate produced also contained high protein content (50.09±0.88%) and may serve as a good protein source.

Tapai is one of the most popular traditional desserts in Malaysia and other Asian countries. Traditionally, tapai is wrapped in a rubber leaf to enhance the smell and increase its palatability. The study focused on identifying the yeasts present before and after the production of glutinous rice tapai wrapped in different rubber leaves clones, namely RRIM 2025, RRIM 2002, PB 260 and PB 350. The identification of the yeast was carried out using API 20C AUX test strips for all rubber leaves clones, glutinous rice tapai wrapped in RRIM 2025, RRIM 2002, PB 260, PB 350 and in a container (control). The results showed that Crytococcus laurentii, Rhodotorula mucilaginosa 2, Candida famata, Rhodotorula minuta were present in rubber leaf clones. While the yeasts that had been identified in tapai wrapped in rubber leaf were Candida guilliermondii, Rhodotorula mucilaginosa 2, Candida parapsilosis and Trichosporon mucoides and only C. guilliermondii was found in the container. The physical properties of the tapai that are wrapped in rubber leaves have a difference in texture, pH value and total soluble solids content compared to the control sample. The tapai sample wrapped in RRIM 2025 and RRIM 2002 had a high total soluble solid content of 45.8±0.14% and 45.78±0.16% °Bx, respectively. Meanwhile, the control sample has the highest pH value and the hardest rice kernels, which were 4.71±0.05 and 218.19±25.39 N, respectively. The results showed that the different yeasts present in the rubber leaf may cause changes in the physical properties of glutinous rice tapai.

Water lily (Nymphaeaceae antares) contains a vast array of phenolic compounds with important natural antioxidative properties that could inhibit the adverse effects of the reactive oxygen species produced in living things. These substances are complex in composition which requires a suitable extraction medium to exploit the phenolic compounds. This study was designed to determine the antioxidative properties in petal and stamen of water lily extracted using different extraction solvents namely ethyl acetate and 60% methanol. Antioxidant properties of the samples were determined using 2,2-diphenyl -1-picrylhydrazyl (DPPH) radical scavenging activity, ferric thiocyanate (FTC) method, thiobarbituric acid (TBA) test, total phenolic content (TPC), total flavonoid content (TFC) and total tannin content (TTC) along with individual flavonoids content using High Performance Liquid Chromatography (HPLC). The yield of extract was found to be the highest in petals extracted with 60% methanol (44.55±1.63%) while stamen extracted with ethyl acetate yielded the lowest (24.50±1.09%). In the DPPH inhibition method, petal extracted with ethyl acetate obtained the highest scavenging activity (89.15±3.62%). FTC analysis revealed that ethyl acetate extract of petal experienced the highest percentage inhibition (76.70±6.48%) while in TBA analysis ethyl acetate extract of stamen exhibited the highest percentage inhibition (70.78±1.39%). Flavonoids such as quercetin, kaempferol, rutin, gallic acid, catechin, epigallocatechin, p-coumaric acid and myricetin were found to be present in ethyl acetate and 60% methanol extracts of water lilies stamen and petal. The information from this study indicates that water lily petals and stamens may be integrated as a good source of nutrients in the food industry, the pharmaceutical industry. If the advantages of water lily are not used and integrated into everyday life for health benefits, it will be a massive waste of natural resources.

The growing consumer demand for healthy food products has turned the interest of industry and researcher in the development of foods enriched with fibre source. Oat and corn cob powder is one of the fibre sources that rich in insoluble fibers, such as cellulose, hemicellulose and lignin which beneficial the consumer in many ways for a healthy diet. The objective of this study was to investigate the effect of oat and corn cob powder incorporation on the sensory acceptability and physicochemical properties of putu piring at different levels of percentage ratio of oat and corn cob (control 0 percent OP: 0 percent CCP A, 0 percent OP: 100 percent CCP B, 30 percent OP:70 percent CCP C, 50 percent OP:50 percent CCP D, 70 percent OP:30 percent CCP E and 100 percent OP: 0 percent CCP F). Physical analysis showed that the colour of putu piring for L* value ranges from 74.17 to 19.76, a* value ranges from 0.23 to 1.90 and b* value ranges from 13.97 to 20.65. Sensory evaluation results showed that putu piring control (formulation A) scored the highest and no significant different (p lower than 0.05) compare followed by 30 percent OP: 70 percent CCP (formulation C) and 50 percent OP: 50 percent CCP (formulation D) in all attributes being evaluated. Proximate analysis showed that putu piring incorporated with oat and corn cob powder has significantly different (p lower than 0.05) in terms of crude fibre content. It is interesting to note that the incorporation of oat and corn cob powder yielded putu piring can improves the nutritive value of the product to become high fibre products.

Mahkota Dewa (Phaleria macrocarpa) also known as God's Crown has been historically used as an indispensable alternative herbal medicine. Microencapsulation is a process whereby tiny particles or droplets are engulfed or enclosed in a coating matrix to produce small capsules. Generally, without microencapsulation, powders are fragile materials that could easily interfere with other components that are difficult to dissolve in water, lose their beneficial properties and decrease shelf life. It is hoped that the microencapsulation would increase the consistency of the powder during storage and maintain its beneficial properties. The goal of this research is to investigate the physicochemical and antimicrobial activity of Mahkota Dewa leaves encapsulated in different concentrations of gum Arabic (GA) and to determine the form of antioxidant and their role and properties. Mahkota Dewa leaves powders were microencapsulated in 0%, 2%, 4%, 6%, 8% and 10% gum Arabic using an ultrasonic spray dryer at 90οC. The microencapsulated Mahkota Dewa leaves (MMDL) samples were subjected to physicochemical and antimicrobial activity. The results showed that the 6% GA MMDL exhibited the highest yield (3.91%) while 0% GA was the lowest yield (1.64%). The highest total phenolic and flavonoid content was exhibited by 2% GA. The highest DPPH inhibition was depicted in 0% GA which indicates the highest antioxidant activity (54.9±0.01%) and is significantly (p

This work aimed to determine the physicochemical and functional properties of starch cassava grown in two different locations in Sabah, namely Tawau and Semporna. In this study, the starch of the cassava was extracted using a wet method and analysed for its physicochemical and functional properties. The total starch content of cassava grown in Semporna showed a higher value (61.21 g/100 g) compared to the sample from Tawau (51.77 g/100 g). No significant difference (p>0.05) observed for starch yield extracted from these two locations. However, there was a significant difference (p0.05) were observed for all gelatinisation and retrogradation properties. This study suggests that location influenced the physical, chemical and functional properties of cassava’s starch.

PurposeThis study aims to investigate the effects of incorporating seaweed composite flour on soft roll dough rheological characteristics and quality.Design/methodology/approachIn this study, wheat flour was substituted with seaweed powder obtained from red seaweed (Kappaphycus alvarezii) at varying proportions (100:0; 99:1; 98:2; 97:3; 96:4; 95:5, 94:6, 93:7 and 92:8) and applied in soft roll production. The effects of seaweed composite flour were evaluated in terms of rheological characteristics, proximate composition and physical properties. The sensory characteristics of the soft rolls was evaluated by 40 untrained panellists by using a hedonic scale.FindingsFarinograph analysis of the soft roll doughs showed that the incorporation of seaweed powder promoted an increase in water absorption, development time and mixing tolerance index, whereas it decreased stability time. Analysis of the proximate composition of the soft rolls showed that protein and carbohydrate contents decreased, but moisture, ash and crude fibre contents substantially increased. Dietary fibre increased with the increase in the proportion of seaweed powder added. The specific volume, bulk density and firmness of the soft rolls ranged from 3.01 to 5.48 cm3/g, 0.18 to 0.33 g/cm3 and 1.86 to 20.63 N, respectively. Sensory evaluation results showed that the mean score of sensory attributes decreased as the proportion of seaweed powder added was increased in the formulations. With regard to the overall acceptability, the panellists preferred the soft rolls with the least amount of seaweed powder added. The results of hedonic tests revealed that the panellists' acceptance decreased as higher amounts seaweed powder were added in the formulations.Originality/valueThis study showed that the seaweed powder of K. alvarezii can be utilised as an ingredient to improve the nutrient composition of baked products.

This study was conducted to investigate the effect of ethanol acidification on the antioxidant properties of Morinda citrifolia leaf (MCL) extract and its catechin derivatives. Four different ethanol (100%, 99.5%, 70%, 50%) with or without 0.5% acetic acid were used for extraction. The antioxidant profile was studied with DPPH radical scavenging activity, FRAP and TPC. The quantification of catechins in MCL was performed using HPLC, and the identification of catechins derivatives was performed with Ultra UPLC-TWIMS-QTOF. The results showed that an extraction solvent composed of 70% ethanol: 29.5% water: 0.5 % acetic acid exhibited the highest DPPH percentage of inhibition (86.12±2.96%) and highest TPC value with 97.80±0.25 mg GAE/g extract, while 100% ethanol acidified with 0.5% acetic acid showed highest FRAP antioxidant power with 1.31±0.05mg FSE/g extract. All eight types of catechins were identified in MCL and the most total catechins were quantified in 70% ethanol: 29.5% water: 0.5 % acetic acid at 153.57mg/g. The catechin derivatives identified included epigallocatechin-3-O-gallate (EGCG), epigallocatechin (4β, 8)-gallocatechin, gallocatechin (4α→8)-epicatechin, catechin-3-O-gallate (CG) and epigallocatechin (EGC). The results suggest that acidification improves the extraction of polyphenols as well as catechin content.

Ciku fruit (Manilkara zapota), also known as sapodilla is an exotic tropical fruit commonly eaten by Malaysians but not as popular as durians, rambutans, and bananas. Due to the short period of storage of ripened ciku fruits, making or converting it to a new end product such as pastille may promote the utilization and promote this tropical climate fruit and provide a wide range of products. Therefore, the purpose of this study was to develop ciku pastille and determine the physicochemical properties and sensory characteristics of ciku pastilles. A total of six formulations of ciku pastille were developed based on the different amounts of ciku purée (10%, 12%, 14%, 16%, 18% and 20% of ciku purée) added. The pastilles were analysed for their texture profile analysis (TPA), water activity (Aw), total soluble solids (TSS), colour analysis, pH, moisture content, ash, fibre and antioxidative activity. Results showed that formulation F (20% ciku purée) illustrated the highest value for moisture (18.59%), ash (2.43%), fibre (2.65%) and scavenging activity (36.58%). The production of this product is highly likely to be commercialised as no synthetic preservatives and artificial colouring were used. The ciku pastille was successfully developed with brownish-orange colour, round-shaped with a diameter of 1.0-1.2 cm, weighing approximately 1.2-1.5 g. Formulation B (12% ciku purée) has 62.7⁰ Brix with pH 4.3, which had an acidic aftertaste and a water activity of 0.54. For colour analysis, L* value (59.76), a* value (23.71) and b* value (34.33) brought the brownish-orange colour for ciku pastille. Formulation B (12% ciku purée) was highly accepted by the panellists and retained its good texture and colour after being kept in the chiller for 2 months at below 18°C. The analysis, therefore, revealed that the physicochemical properties of the ciku pastille produced were enhanced and found to be rich in antioxidants and accepted by the panellists. Ciku fruit may consequently be a possible natural ingredient for pastille production, thus promoting its use in the food sector.

The objective of this present work is to determine the chemical compositions and volatile compounds of Sabah indigenous durian (Durio dulcis Becc.). The results obtained showed that durian had 56.1% moisture, 3.5% protein, 2.8% fat, 1.4% ash, 36.26% carbohydrate, 6.6% dietary fiber and 7.0 µg of vitamin A. The mineral analysis results indicated that Durio dulcis is a good source of potassium, magnesium and phosphorus. Protein analysis found a total of sixteen major amino acids present in durian namely aspartame, serine, glutamin acid, glycine, histidine, arginine, threonine, alanine, proline, thyrosine, valine, methionine, lysine, isoleusine, leusine and phenylaline. A total of thirty-three individual fatty acids components were analysed. Among those, palmitic acid was a predominant saturated fatty acid while oleic acid was the most abundant unsaturated fatty acid. The composition of fatty acids showed that total of monounsaturated fatty acids, polyunsaturated fatty acids and saturated fatty acids were 56.7%, 6.7% and 36.6% respectively. A total of thirty-one volatile compounds were identified, among which alcohols, esters, sulphur-containing compounds and ketones were found to be the major constituents.

Clitoria ternatea (L.) (Bunga Telang), is a well-known plant used as food colouring through its flowers. The plant’s leaves are also being used as medicinal ingredients to cure several diseases. However, the medicinal properties were not being explored extensively which could diversify the plant’s usage. The research was conducted on the inclusion of C. ternatea leaves to enhance the physicochemical properties and sensory acceptability of herbal pastille. Five different formulations of C. ternatea leaves pastille were developed with different percentages of C. ternatea leaves namely 2.5%, 5.0%, 7.5%, 10.0% and 12.5%. Physicochemical properties (antioxidant properties, colour profile, texture, moisture, sugar, fat, protein, ash, calorie and texture profile) and the sensory acceptability of C. ternatea leaves pastille was observed. The results show that the highest °Brix value was observed in sample D (74.20°) while water activity was higher in sample B (0.73). The analysis showed that the control sample exhibited a high hardness value (2641.70 N), springiness and cohesion values (0.87 N), gumminess value (2338.75 N), chewiness value (2089.71 N) and resilience value (0.44 N) to that of C. ternatea leaves herbal pastille. Sample E was found to contain a high TFA value (1.45), TPC value (23.32 GAE mg/g), and DPPH value (27.72%). Based on overall acceptance, the sensory analysis showed that the products were accepted by the panellists. Thus, the study revealed that the physicochemical properties of developed herbal pastille were being enhanced and found to be rich in antioxidants and accepted by the panellists.

Natural plant products are becoming more and more essential in helping to promote safe well-being worldwide. This leads to a substantial rise in the consumption of various herbal teas. The presence of beneficial bioactive ingredients such as flavonoids may be correlated with Napier grass herbal tea having health benefits associated with their intake. Studies have shown that herbal teas have preferentially high antioxidant activity due to the presence of flavonoids in them. The purpose of this study was to identify the Napier grass herbal teas flavonoids prepared under different conditions. Napier grass herbal teas have been formulated using green tea and black tea processes, respectively. The tea samples were extracted in water (95°C, 30 mins) and 60% (v/v) aqueous methanol (30 mins), respectively. Approximately, 1% (v/v) aqueous acetic acid solution (solvent A) and acetonitrile (solvent B) were used as the mobile phase. The flow rate was adjusted to 0.7 mL/min, the column was thermostatically controlled at 28°C, and the injection column was kept at 20 μL. HPLC chromatograms were detected using a photodiode array UV detector at 272 nm. Gallic acid, P-coumaric acid, catechin, epigallocatechin gallate, quercetin, rutin, myricetin and kaempferol were found in both Napier grass water and methanolic extracts, respectively. The findings suggested that the HPLC techniques are ideal for the detection and identification of flavonoids in Napier grass teas

Nutraceutical and pharmaceutical industries have been increasingly engaged in finding natural alternatives compounds as potential antioxidants. The use of phytochemicals is introduced as a good source of natural antioxidants. Murayya koenigii leaves, commonly used in cooking and traditional medicines have been examined for their remarkable antioxidant potential, yet still, it remains an understudied herb. Therefore, this study aimed to determine the antioxidant properties and flavonoids profile in M. koenigii leaves extracted using; solvent assisted extraction (SAE), microwave assisted extraction (MAE) and ultrasonic assisted extraction (UAE). The antioxidant properties of M. koenigii were analysed qualitatively and quantitatively using high performance liquid chromatography (HPLC). M. koenigii leaves extracted using the UAE method have responded strongly towards a 2, 2-diphenyl -2-picryl-hydrazyl DPPH assay with the highest inhibition (%) of 78.00±1.00. Using the ferric thiocyanate (FTC) and thiobarbituric acid (TBA) method assays, the M. koenigii leaves with the lowest absorbance were assigned as a sample with the highest antioxidant activity. The M. koenigii leaves extracted using UAE had the lowest absorbance with 0.01±0.00. In the TPC assay, the MAE method showed the highest total phenolic content (120.60±14.81 mg GAE/g sample). The TFC assay demonstrated that MAE methods have the highest total phenolic content (93.38±4.33 mg QE/g sample. The M. koenigii leaves extracted by MAE showed the highest gallic acid, catechin, epigallocatechin gallate, rutin and kaempferol concentration (mg/L). M. koenigii leaves subjected to SAE extraction has the highest concentration of p-coumaric acid, myricetin and quercetin (mg/L). This study found that M. koenigii leaves extracted using UAE exhibited better antioxidant activities than that of MAE and SAE. These useful findings have managed to narrow the knowledge gap regarding the effects of different extraction methods on the antioxidant property of M. koenigii.

Spent Coffee Ground (SCG) comprises a range of functional components with high antioxidant potential and health benefits. Although SCG has many advantages, antioxidant values obtained using enzymes is still scarce. Several studies reported that the use of enzyme-assisted extraction techniques could improve the detection of antioxidant compounds in samples of by-products. In this study, SCG was extracted using enzymeassisted techniques for different periods (0, 20, 40, 60, 80 and 100 mins). The total antioxidant capacity was measured using Peroxidase, Catalase, Ferric reducing antioxidant power (FRAP), Superoxide radical scavenging assay (SRSA) and 2,2-diphenyl-2- picrylhydrazyl (DPPH) analysis. 60 mins extraction was found to exhibit antioxidant activity in DPPH, SRSA, Catalase and Peroxidase of 84.18±7.01%, 32.83±6.75%, 76.65±5.52% and 75.01±7.67%, respectively. Besides that, the extraction also gave a high amount of content with 267.17±26.69 mg TAE/mL and 58.30±2.36 mg QE/g in total phenolic content and total flavonoid content respectively. Flavonoids such as quercetin, kaempferol, rutin, gallic acid, catechin, epigallocatechin, p-Coumaric acid and myricetin were found to be present in SCG extract. A high amount of catechin was found in all periods of extraction, whereby 60 mins had the highest concentration of 1741.65 mg/L, while 40 mins had the lowest concentration which was 389.85 mg/L. In conclusion, 60 mins extraction using a pectinase-assisted method was the best extraction period. Additionally, the individual flavonoid catechin in SCG showed that it has potential as an antioxidant. Finally, there is a strong correlation between antioxidative activity and both phenolic and flavonoid content

Although the fried products are delicious with a tenderizing effect on the crust due to the presence of fat, over-consumption of fried products causes health problems, especially coronary diseases. The tendency of proteins in film formation and thermal gelation to reduce the absorption of oil in fried products is emphasized. The purpose of this study was to determine the reduction of oil in deep-fried squid by the incorporation of protein hydrolysate and to discuss its effect. Yellowstripe scad protein hydrolysate was produced using Alcalase® enzyme. Fat content was determined using the Soxhlet method, subsequently substituted into a formulation for oil uptake calculation. The viscosity of batter was determined using a rheometer. The viscosity of the batter and batter pick-up was found to be directly proportional, showing a significantly reduced pattern from 0 to 20%. Incorporating 10% of fish protein hydrolysate successfully decreased oil absorption by 17.35±0.73% with a good water retention rate of 38.46%. The addition of the Yellowstripe scad fish protein hydrolysate modified the size and shape of the pore. Sensory acceptance portrayed no significant difference among the three samples (0%, 5% and 10% of incorporation), indicating that panellists were able to accept samples incorporated with fish protein hydrolysate. The findings of this study showed that Yellowstripe scad protein hydrolysate can minimize the uptake of oil in fried seafood products and thus could increase the economic value of the Yellowstripe scad fish.

The food industry needs a creative approach to innovation in order to produce revolutionary materials, innovations and fresh, nutritious, sustainable food products. Tempeh is a traditional meal prepared using Rhizopus oligosporus to ferment dehulled and cooked soybeans to a compact and sliceable cake. Because of their high content of proteins, carbohydrates, lipids, and minerals, energy bars are snacks offering good sensory and nutritional properties. Canavalia ensiformis (Kacang Koro), is an underutilised legume that contains up to 32% of protein. To our knowledge, no prior studies have studied regarding tempeh and energy bars, especially in Malaysia. This research aimed to evaluate both the physicochemical properties and sensory acceptance of the C. ensiformis tempeh energy bar. Energy bars of six formulations (Formulation A – E) were produced using different percentages of C. ensiformis tempeh namely 0%, 4.8%, 9.5%, 14.3%, 19.0% and 23.8%. The physical characteristics of the energy bar were analysed based on colour profile analysis, texture analysis and also the pH value, while the chemical characteristics were analysed based on proximate analysis, calories, mineral content, and toxicity analysis. The results showed that the energy bars consist of moisture content of 9.29-13.09%, ash of 0.99-1.56%, crude fibre of 1.82-7.27%, protein of 4.93-10.34%, crude fat of 12.36-15.97%, carbohydrate of 58.91-64.94%, and calorie content of 4627.55 - 5267.80 cal. Energy bars with 23.8% of C. ensiformis tempeh exhibited highest in moisture and protein, moderate in ash and fibre and showed no significance in carbohydrate and fat contents. The taste and overall acceptability indicate that formulation A showed the best acceptance among the prepared formulations. The utilization C. ensiformis tempeh could diversify the usage of C. ensiformis in the food industry, hence promoting their application.

Malay cherry fruit or locally known as Ceri Terengganu (Lepisanthes alata Leenh.) is a local tropical exotic fruit and it is native to Malaysia. The Ceri Terengganu tree is widely distributed in the east coast of Peninsular Malaysia particularly in Terengganu, Pahang and Johor and commonly cultivated as an ornamental plant in the villages and gardens. A limited number of studies had been done on the proximate analysis and postharvest quality of Ceri Terengganu fruit, but the studies on the antioxidant and antibacterial activities of Ceri Terengganu fruit extract are still lacking. Hence, this study aimed to determine the antioxidant and antibacterial activity in the peel, flesh and seed extracts of Ceri Terengganu. The Ceri Terengganu was extracted using 60% ethanol and the total phenolic content (TPC), total flavonoid content (TFC), total monomeric anthocyanins, antioxidant and antibacterial activities were measured using standard methods. The results showed that the seed of Ceri Terengganu had the highest amount of TPC, TFC and antioxidant activity, followed by the peel and flesh extracts whilst the peel extract had the highest total monomeric anthocyanins content. Furthermore, all three extracts of Ceri Terengganu showed inhibition against selected pathogens tested. In conclusion, the seed of Ceri Terengganu possessed the greatest potential to be explored as a source of natural antioxidant and antibacterial agent in the food industry, and thus warrant further investigation.

Spent coffee ground (SCG) is the by-product of coffee processing that is produced up to 45% in coffee beverage and instant coffee processing and is believed to contain high amounts of antioxidants. Despite the fact that SCG has exerted many advantages, the information on obtaining the antioxidant values using enzymes are still scarce. The objective of the study was therefore to determine the total phenolic and flavonoid content of antioxidant activity and antimicrobial activity in Arabica SCG extracted using pectinase at different pH values. Arabica SCG was extracted using pectinase at pH 3, pH 4, pH 5, pH 6 and pH 7 and analyzed for its anti-microbial activity and antioxidant properties (DPPH, FRAP, total phenolic and total flavonoid content along with individual flavonoids using HPLC). Arabica SCG was extracted using pectinase at pH 3, pH 4, pH 5, pH 6 and pH 7 and analyzed for its antimicrobial activity and antioxidant properties (DPPH, FRAP, total phenolic and total flavonoid content along with individual flavonoids using HPLC). The result showed that the antioxidant capacity of the SCG extract at pH 4 exhibited higher DPPH and FRAP values. The total phenolic and flavonoid content exhibited in (1.38±4.42, mg GAE/g sample and 22.57±0.27 mg QE/g of dry sample). Flavonoids namely quercetin, kaempferol, rutin, gallic acid, catechin, epigallocatechin gallate, pcoumaric acid and myricetin were present in all samples at various levels. The SCG in pH 7 extract showed the highest concentrations of the individual flavonoid compound in the sample and the highest inhibition zone on Pseudomonas aeruginosa and Staphylococcus aureus. Thus, the optimum pH of the pectinase which is pH 4-5 had provided the highest yield in antioxidant capacity and activity of the Arabica SCG extract with this extraction method that can be used for food preservation as well as in the nutraceutical industry.

Deep-fried food is a fast and convenient way to prepare food that imparts desirable sensory characteristics of colour, flavour and in particular, a smooth texture, yet has been labelled as not healthy by consumers. Incorporation of other ingredients in the formulation of the batter could reduce the fat absorption in deep-fried foods. This research was aimed to determine the physicochemical and sensory properties of Brownstripe red snapper protein hydrolysate (BRSPH) and its utilisation in reducing the oil intake of deep-fried foods. The BRSPH were extracted using the enzymatic method utilizing Alcalase® as the working enzyme. Batter formulations were prepared by adding 0%, 2%, 4%, 6% and 8% of BRSPH into the sample mixtures. Addition of BRSPH into the batter was found to increase hardness and crispness of deep-fat fried battered squids. The fat content of the deep-fat fried battered squids with 8% BRSPH powder was found to be the lowest compared to those added with 2%, 4% and 6%, while sample without BRSPH powder was the highest (30.15%). Deep-fat fried squids with 4% of BRSPH powder showed the best acceptability scores in terms of crispness, taste and overall acceptability, but no significant differences were determined in the crispness between deep-fat fried squids with and without BRSPH. The findings indicate that enzymatic hydrolysis using Alcalase® has the potential to yield BRSPH with a high degree of hydrolysis (98.19%), low molecular weight (10-15 kDa), and low oil binding capacity (2.38 g oil/ g protein). Enzymatic hydrolysis proved to be a viable technique to produce protein hydrolysate which able to reduce the oil uptake and healthier with high acceptability of the end products. Research on how to optimize the use of BRSPH with a high economic, nutritional and industrial potential of understated resources would allow for the implementation of manufacturing practices to enhance the use of resources and increase the value of the by-product. This approach will offer the potential use of BRSPH for the production of batter formulation which is efficient in the reduction of oil uptake

Spent coffee grounds (SCG) are the primary by-product of coffee production which still contains functional properties with high natural antioxidant components. It can be extracted using ultrasonic-assisted techniques and then integrated into baking products such as cookies. This research is aimed to measure the physicochemical and sensory acceptability of cookies incorporated with SCG extract. Extraction of SCG was done using water through ultrasonic extraction following incorporated SCG water extract into cookies. Six different formulations were developed using different amount of SCG extract (A-control 0%, B-0.27%, C-0.52%, D-0.80%, E-1.07% and F-1.33%). Physicochemical properties (antioxidant properties, colour profile, texture, moisture, sugar, fat, protein, ash, calorie and texture profile) and the sensory acceptability of SCG extract cookies was observed. Total phenolic content (TPC) was used to quantify the antioxidant content while the antioxidant activity was measured using 2,2-diphenyl-2-picrylydrazyl radical scavenging activity assay (DPPH) and ferric reducing antioxidant power (FRAP) assays. Results show that decreasing in lightness (L*) and yellowness (b*) with the increasing percentage of SCG extract were observed. In contrast with L* and a* value, the b* value of cookies was increased with the increasing percentage of SCG extract. Formulation E (1.07% SCG extract) showed the highest percentage in almost all proximate analysis such as 6.49±0.39 moisture, 2.11±0.22 ash, 20.42±0.74 crude fat, 8.13±0.05 crude protein and 4.37±0.04 crude fibre content. The highest amount of antioxidant content was depicted by the formulation D cookie (1.72±0.04 mg GAE/g). The best antioxidative activity was found in formulation E (7.80±0.27 DPPH inhibition and 0.02 abs by FRAP analysis). Sensory acceptance revealed that formulation E (1.33%) cookies were more accepted by the panellists. It is interesting to note that SCG can serve as one of the ingredients for cookie production that showed significant effects on the physicochemical and organoleptic properties of the final products.

Every good food comes with its negative side, and ‘Kacang Koro’ is one of them. Although ‘Kacang Koro’ contains a lot of protein, it also has a negative side, such as an anti-nutritional factor. These anti-nutrient factors need to be removed in order to produce nutritious food. This study was aimed to determine the effect of pre-treatment (soaking, soaking-boiling, soaking-roasting, soaking-microwave) on physicochemical composition and sensory acceptability in ‘Kacang Koro’ energy bar. The energy bars were subjected to physical analyses, proximate analysis, calorie content, phytochemical analyses and sensory analysis. All the different treatments of ‘Kacang Koro’ energy bar showed moisture content of 8.29 - 12.42%, ash of 1.09 - 1.11%, crude protein of 7.51 - 10.14%, crude fat of 12.75 - 17.77%, crude fibre of 2.78 - 3.62%, and carbohydrate of 57.19 - 63.91%. The calorie content was found to be 484.81 - 511.29 kcal/100 g. The oxalate content in all ‘Kacang Koro’ pre-treated is ranged between 29.81 - 45.1 mg/kg. The phytic acid content is between 0.15% - 0.37% and the tannin content is in the range of 25.53 L - 52.30 mg GAE/L. Energy bar with soaking-microwave pretreated ‘Kacang Koro’ has higher value in crude protein, crude fat, and crude fibre but moderate in moisture and carbohydrate content, meanwhile lower in ash content. Sensory acceptability tests showed the sweetness, taste and overall acceptability of the samples were significantly different between ‘Kacang Koro’ energy bars using soaking compared to soaking-microwave pretreatment. The pre-treatment of ‘Kacang Koro’ may boost the use of these ‘Kacang Koro’ in the food industry and promote their application.

Clitoria ternatea flowers are known as butterfly pea flowers which contain many bioactive compounds and can be found in tropical countries. However, the bioactive compounds are easily lost when exposed to various environmental conditions. Encapsulation technologies are introduced to provide maximum protection to the encapsulated bioactive compounds. The main objectives of this study were to determine the physicochemical properties of C. ternatea flowers encapsulated in gelatine prepared using different encapsulating methods and the microbiological properties of the best encapsulating methods for C. ternatea flowers with gelatine. In this study, the moisture contents for ultrasonic spray dried powders recorded the lowest (5.94±0.44%) while samples of convection oven recorded the highest (14.33±1.30%). However, the ultrasonic spray dried powders demonstrated the highest total flavonoid contents, but convection oven dried powders showed the lowest. The results for total anthocyanin contents were similar to total flavonoid contents. The highest encapsulation efficiency based on anthocyanin contents was found in freeze dried powders (95.75±0.24%). These results showed the same antioxidant activity (DPPH assay) with the highest percentage inhibition of freeze dried powders and the lowest percentage inhibition of ultrasonic spray dried powders. The phytochemical functional group that revealed from Fourier Transform Infrared spectroscopic (FTIR) analysis also indicate the presence of high amount of phenolic compounds in freeze dried powders although with ‘collapse building’ shape with fibrillary structure. The freeze dried powder showed the highest L* value (45.62±0.54), yet ultrasonic spray dried powders highest a*, b* and C* value. Thus, the analysis for microbial properties was carried out on freeze dried powders as freeze dryer was chosen as the best encapsulating methods. The freeze dried powders showed inhibition against gram positive and gram negative bacteria such as Bacillus cereus, Staphylococcus aureus, Escherichia coli and Salmonella enterica and fungi such as Aspergillus niger and Candida albicans. The current study demonstrated the potential of using gelatine to encapsulate technique to retain antioxidant compounds in gelatine encapsulated C. ternatea flowers. This finding provides useful information on the use of different encapsulated methods for the development of functional food products for gelatine encapsulated flowers of C. ternatea.

The tuber of cassava is used as raw materials in the bakery, food, pharmaceutical and garment industries. The nutritional value of cassava roots is important because they are the main part of the plant consumed in developing countries. However, there is much variation in the nutrient quality of the cassava root depends on the several factors, such as geographic location, variety, age of the plant, and environmental conditions. This study was performed to compare and provide information on physicochemical and functional properties of cassava flour planted in two different districts in Sabah, Malaysia, namely Tawau and Semporna. Proximate analysis showed significant differences (p

Toothpony (Gazza minuta) is not typically used for human consumption due to its small size and low meat content, which is widely used as a fish meal. It is stated to be high in protein content and may be suitable for fish protein hydrolysate (FPH) production. Deepfat fried foods contain significant amounts of fat, in some cases up to one-third of the total food weight after frying. Toothpony FPH with low oil-binding ability may reduce the amount of oil consumed by deep-fried products. The objective of this research was to measure the physicochemical properties of Toothpony FPH and its utilisation in reducing the oil intake of deep-fried food. Toothpony FPH was obtained using the enzymatic method successfully. Batter formulations are prepared by adding 0%, 2%, 4%, 6% and 8% of Toothpony FPH into the sample mixtures. The present study indicates the degree of hydrolysis of Toothpony FPH determined by trichloroacetic acid (TCA) method was found to be 98.02%. Toothpony FPH's molecular weight distributions ranged from 7 to 175kDa and FTIR's transmission spectrum indicated the presence of amide I and amide II compounds (1654 and 1535cm-1 ). Oil binding capacity of Toothpony FPH was found to be low, which was 1.9 g oil/ g protein. This study indicated that 4% of Toothpony FPH produced the optimum amount to be incorporated in batter in order to best reduce oil uptake. In sensory evaluation, deep-fat-fried squid incorporated with 8% of Toothpony FPH showed the highest acceptance in all attributes, which were colour, crispness, oiliness, taste and overall acceptability. This approach offers the potentials use of Toothpony fish FPH for the production batter formulation which is efficient in the reduction of oil uptake.

The objective of the research was to investigate the physicochemical characteristics of Tarap fruit starch. In this study, young Tarap fruit starch was extracted and the percentage of total starch, resistant starch, amylose and amylopectin were determined. Scanning electron microscope was used to evaluate the morphological features of the starch granule. Swelling, pasting, gelatinization, retrogradation and in vitro digestibility were also investigated. A total of 17.85% starch was successfully extracted from unripe Tarap fruit, whereas the amount of total starch and resistant starch were 89.14% and 47.82%, respectively. The amounts of rapid digestible starch and slowly digestible starch were 6.58% and 23.25%, respectively. Results found that the amylopectin content was higher than amylose (77.15% and 11.97%). The starch granules were round and polygon in shapes with smooth surfaces. The average of starch granules size was range from 6.50 to 8.30 μm with 7.4 μm of mean granule diameter. Pasting properties showed that peak viscosity was observed at about 6.5 min at 73.5oC. Tarap starch gelatinization temperatures (onset, 71.63°C; peak, 74.56°C; conclusion, 78.24°C) and enthalpy of gelatinization (ΔHgel) (3.74 J/g) were higher while the retrograded starches show lower retrogradation temperature and enthalpy than native starches. Unripe Tarap starch showed good potential to be utilized as adhesives and thickener for industrial applications.

Pastille is a type of small round sweet confectionery product categorized in the Gummy Candies family that can be sucked or chewed. The goal of this research was to determine the physicochemical features and sensory profile of five pastille samples (F1, F2, F3, F4 and F5) produced with a mixture of belimbing buluh fruit juice and distinct concentrations of Arabic gum and gelatine. The pastilles were developed from the mixture of belimbing buluh fruit juice, sorbitol, sugar syrup using separate concentrations of Arabic gum (0%, 4%, 8%, 12% and 16%) and gelatine. The physicochemical analysis shows that, F1 using 16% of gelatine obtained 19.48% moisture content, 0.25% crude fat, 20.60% crude protein, 0.45% crude fiber, 0.34% ash, 58.87% carbohydrates and 3475.3 kcal calorie content, while F5 using 16% of Arabic gum obtained 13.52% moisture content, 0.688% crude fat, 0.69% crude protein, 0.188% crude fiber, 0.92% ash, 84.01% carbohydrates and 3174.1 kcal calorie. The physical analysis showed that belimbing buluh fruit pastilles properties were influenced by the addition of Arabic gum and gelatine. The sensory study showed that the overall acceptance for sensory analysis showed that sample F3 (3.87) and sample F5 (3.63) were moderately liked by the panellists. However, more effort is needed in order to improve the quality of the belimbing buluh fruit pastilles, using other ingredients.

Tea fermentation relates to the enzymatic oxidation of the leaves of the tea and the formation of dark pigments. This method is usually conducted in a setting where temperature and humidity can be regulated. However, the impact of fermentation time on the antioxidant characteristics of Napier grass herbal tea is still unanswered. This study aimed to determine the effect of fermentation time on antioxidant properties of Napier grass black tea. Napier grass was subjected to fermentation for 3, 6, 9, 12 and 24 hrs. Fresh and dried samples were extracted in water (95°C, 30 mins) and the extracts were then analysed by total phenolic content (TPC) assay, total flavonoid content (TFC) assay, diphenyl-picryl-hydrazyl (DPPH) assay, Ferric reducing antioxidant potential (FRAP) assay, ferric thiocyanate (FTC) method and thiobarbituric acid (TBA) method. Sample fermented for 9 hrs showed the highest results in TPC (18.32±0.38) and FRAP assay (91.00±2.78). Whereas, the highest flavonoids and antioxidant activity were found in dried sample without fermentation (control) in TFC (97.82±13.00) and DPPH (90.02±1.11). However, samples fermented for 3 to 9 hrs showed no significant difference with that of the fresh sample in TFC and DPPH indicating 3 to 9 hrs fermentation time did not affect the antioxidant properties of Napier grass. In FTC and TBA, all the fermented samples showed lower antioxidant activity than that of fresh samples and control. The results suggested that 9 h of fermentation time is suitable for the preparation of Napier grass black tea as it exhibited high antioxidant properties in TPC, TFC, DPPH and FRAP assays.

This study aimed to determine the effect of steaming time on antioxidant properties of Napier grass green tea. Napier grass was subjected to steaming for 1, 2, 3, 4, 6, 8 and 10 mins. Fresh and dried samples were extracted in water (95°C, 30 mins) and the extracts were then analysed by total phenolic content (TPC) assay, total flavonoid content (TFC) assay, diphenyl-picryl-hydrazyl (DPPH) assay, Ferric reducing antioxidant potential (FRAP) assay, ferric thiocyanate (FTC) method and thiobarbituric acid (TBA) method. Sample steamed for 8 mins showed the highest TPC (18.32±0.26), TFC (152.71±5.74) and 109.88±5.44 in FRAP assay. High antioxidant activity was found in sample steamed for 3 to 10 min (81.63±1.19 to 83.50±1.10) in DPPH which were not significantly different with the fresh sample indicating steaming can retain the phytochemical compounds. Samples undergone 6 to 10 mins steaming time were found to have high lipid peroxidation in ferric thiocyanate (75.02±2.96 to 81.01±6.68) and thiobarbituric acid (85.99±1.56 to 86.21±1.44) assays. The results suggested that 8 mins of steaming time is suitable for Napier grass green tea as it exhibited the greatest antioxidant properties

Seaweed has a great potential to be used as an important ingredient in food processing, as it contains a significant content of soluble polysaccharides and has a potential function as a dietary fiber. In this study, seaweed powder (Kappaphycus alvarezii) was incorporated (2–10%) with wheat flour for the production of muffins. The effect of seaweed composite flour on the quality of muffins was investigated using various techniques, such as texture profile and proximate analyses, as well as sensory evaluation. Modification of the muffin formulation to include seaweed powder improved the composition of ash, crude fiber, and moisture content, while the protein and carbohydrate levels decreased, compared to the control sample. An increase in the seaweed component reduced the muffin height, volume, and specific volume. According to the experimental texture profile analysis data, the seaweed affected the textural characteristics of the muffins, increasing the hardness and decreasing the springiness. Sensory evaluation revealed that seaweed powder could be used in the muffin formulation up to 6%, without significantly impacting the color, aroma, and taste attributes relative to the control sample. For overall acceptability, however, the panelists preferred muffins without seaweed powder.

The increasing demand in developing countries for alternative protein sources, coupled with the relatively high cost of importing protein, has led to the search for alternatives, particularly for novel legumes native to the tropics.Canavalia ensiformisor Jack Bean (Kacang Koro) could provide adequate protein sources for human consumption if the presence of various antinutrients can be reduced. The most cost-effective processing technique for the detoxifying method is the soaking process. Therefore, the aim of this study was to determine the effect on the nutritional, antinutritional and mineral content ofC. ensiformisof soaking time and fermentation. The samples were treated by soaking in 1% sodium bicarbonates (NaHCO3) solutions for 12 h, 24 h and 36 h (at chilled temperature, 10 °C) and fermented (40 °C). The treated samples were analysed for their proximate values and antinutrient factors (hydrogen cyanide (HCN), phytic acid, tannin, saponin and oxalate content). The results showed that proximate values were not significantly affected by soaking treatment and fermentation. After 36 h of soaking in 1% of NaHCO3solution, the HCN level inC. ensiformisand fermentation process, the HCN level to be reduced to 7.43 ± 0.76 mg/kg while phytic acid was reduced to 0.64±0.00, saponin to 1.27 ±0.01, tannins to 0.01± 0.001 and oxalate to 39.96 ± 5.85. These results suggest good prospects for substitutingC. ensiformisfor any existing protein source as the antinutrient factors could be reduced to an acceptable level. The soaking ofC. ensiformisin 1% NaHCO3coupled with fermentation would also allow the use ofC. ensiformisin food production.

An aqueous extraction of moringa (Moringa oleifera) leaves were prepared as the edible coats for keeping the quality of the giant freshwater prawn (Macrobrachium rosenbergii). In addition, the antioxidant properties and activity; total phenolic content (TPC), total flavonoid contents (TFC), free radical scavenging activity (DPPH), and ferric reducing antioxidant power (FRAP) of moringa leaves were also determined. The phenolic compounds and antioxidant properties in the moringa leaves are low; 16.14 mgGAEg-1 for TPC; 5.57 mgQEg-1 for TFC; 1.36 mgTEg-1 for DPPH; and 3.05 mgTEg-1 for FRAP. The experiment was further conducted by coating the M. rosenbergii with moringa leaves extraction before chilled storage at 4°C for 15 days. Moringa leaves extraction were effectively reduced the microflora count in M. rosenbergii (P

Histidine is precursor for histamine (4-(2-aminoethyl) imidazole) formation through a process called decarboxylation with the aid of histidine decarboxylase enzyme possessed by particular bacterial species. Since storage temperature is highly influenced the formation of histamine, a study regarding histamine and bacterial count was conducted. Long-tail tuna was stored at -20, 4, 15, 25 and 37 ̊C and analysis of bacteria and histamine was done for every 48 hours till reaching 196 hours for freezing and chilling storage temperature. Histamine determination and bacterial count was taken for every 0, 6, 12,18 and 24 hours for 15, 25 and 37 ̊C. At 0 day of storage for -20̊ C, gut and non gut fish samples showed histamine level of 3.496 mg/100. Reduction of histamine levels took place after 48 hours of storage but increased later throughout the storage period up to 8 days, reaching a value of 4.84 and 3.78 mg/100 g for gut and non gut tuna, respectively. Similar trend of result was seen at 4 ̊C where a gradual increase of histamine concentration was observed throughout the storage period. However, the histamine level in gut tuna exceeded the safety level at day 8. At temperature 15 and 25 ̊C, the gut fish meat is not safe for consumption at 24 hours. Meanwhile, the safety limit can be seen within 12 hours and 18 hours of storage in gut and non gut fish stored at 37 ̊C, respectively. For bacterial cell enumeration, at -20 ̊C, the initial count of bacteria is 5.4 Log CFU/g for both fish condition. The count then slightly increased to 6.28 and 5.91 for gutted and non gutted respectively at 48 hours till reaching last storage time. Similar trend was recorded at 4, 15, 25 and 37 ̊C from time to time. However, bacterial count is largely seen at 37 ̊C. This may be due to the presence of spoilage bacteria that can deteriorate the quality of fish.

Antioxidative activity and total phenolic compounds of root, leaf and petiole of four accessions of Centella asiatica (L.) Urban, namely CA 01, CA 05, CA 08 and CA 11, were evaluated. Antioxidative activity of the extracts was measured using the ferric thiocyanate (FTC) method and thiobarbituric acid (TBA) test. The antioxidative activities were then compared with that of α-tocopherol (natural antioxidant) and butylated hydroxytoulene or BHT (synthetic antioxidant). The results showed that CA 01 and CA 05 had the highest antioxidative activities among the accessions tested. Results also showed that both leaf and root of C. asiatica had high antioxidative activity, which was as good as that of α-tocopherol. The total phenolic content, determined according to the Folin–Ciocalteu method, varied from 3.23 to 11.7 g/100 g dry sample, and showed strong association (r2=0.90) with antioxidative activity. The results suggest that phenolic compounds are the major contributors to the antioxidative activities of C. asiatica.