7 results on '"M. Sofía Urbieta"'
Search Results
2. Thermophiles in the genomic era: Biodiversity, science, and applications
- Author
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M. Sofía Urbieta, Saleha Shahar, Lee Li Sin, Kian Mau Goh, Edgardo Ruben Donati, and Kok-Gan Chan
- Subjects
Hot Temperature ,Archaeal Proteins ,Bioengineering ,Applied Microbiology and Biotechnology ,Ciencias Biológicas ,Industrial Microbiology ,Bacterial Proteins ,Crenarchaeota ,THERMOPHILES ,Hot spring ,APPLICATIONS ,biology ,Bacteria ,Ecology ,Thermophile ,Ecología ,biology.organism_classification ,Archaea ,Hyperthermophile ,Enzymes ,EXTREMOPHILES ,Biodegradation, Environmental ,Metagenomics ,Aquificae ,Biofuels ,Thermotogae ,Sulfolobus solfataricus ,Metagenome ,BIODIVERSITY ,CIENCIAS NATURALES Y EXACTAS ,Biotechnology - Abstract
Thermophiles and hyperthermophiles are present in various regions of the Earth, including volcanic environments, hot springs, mud pots, fumaroles, geysers, coastal thermal springs, and even deep-sea hydrothermal vents. They are also found in man-made environments, such as heated compost facilities, reactors, and spray dryers. Thermophiles, hyperthermophiles, and their bioproducts facilitate various industrial, agricultural, and medicinal applications and offer potential solutions to environmental damages and the demand for biofuels. Intensified efforts to sequence the entire genome of hyperthermophiles and thermophiles are increasing rapidly, as evidenced by the fact that over 120 complete genome sequences of the hyperthermophiles Aquificae, Thermotogae, Crenarchaeota, and Euryarchaeota are now available. In this review, we summarise the major current applications of thermophiles and thermozymes. In addition, emphasis is placed on recent progress in understanding the biodiversity, genomes, transcriptomes, metagenomes, and single-cell sequencing of thermophiles in the genomic era. Fil: Urbieta, María Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentina Fil: Donati, Edgardo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentina Fil: Chan, Kok Gan. University of Malaya; Malasia Fil: Shahar, Saleha. Universiti Teknologi Malaysia; Malasia Fil: Sin, Lee Li. Universiti Teknologi Malaysia; Malasia Fil: Goh, Kian Mau. Universiti Teknologi Malaysia; Malasia
- Published
- 2014
3. Archaeal and bacterial diversity in five different hydrothermal ponds in the Copahue region in Argentina
- Author
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Ángeles Aguilera Bazán, M. Alejandra Giaveno, Elena González Toril, M. Sofía Urbieta, and Edgardo Ruben Donati
- Subjects
Biogeochemical cycle ,Chemical Phenomena ,Otras Ciencias de la Tierra y relacionadas con el Medio Ambiente ,Argentina ,Biodiversity ,ENVIRONMENTL 16S rRNA CLONE LIBRARIES ,Applied Microbiology and Biotechnology ,Microbiology ,Hot Springs ,Genes, Archaeal ,Molecular ecology ,Ciencias de la Tierra y relacionadas con el Medio Ambiente ,FISH ,RNA, Ribosomal, 16S ,parasitic diseases ,Extremophile ,Ponds ,Phylogeny ,Ecology, Evolution, Behavior and Systematics ,Bacteria ,Geography ,biology ,Ecology ,fungi ,Sulfur cycle ,Acid mine drainage ,biology.organism_classification ,Archaea ,EXTREMOPHILES ,Thermoplasmatales ,SULPHUR CYCLE ,GEOTHERMAL ENVIRONMENT BIODIVERSITY ,Water Microbiology ,CIENCIAS NATURALES Y EXACTAS - Abstract
Copahue is an acidic geothermal volcanic region in the northwest corner of Neuquén Province, Argentina. In the area, there are various ponds, pools and hot springs with different temperatures, pH values and levels of anthropogenic influence. In this study, the prokaryotic biodiversity of five representative ponds was studied by using two complementary molecular ecology techniques: phylogenetic analysis of 16S rRNA bacterial and archaeal genes and FISH (or CARD-FISH) for quantitative estimation of biodiversity. The results, supported by multivariate statistical analysis, showed that the biodiversity in Copahue ponds seemed to be determined by temperature. High temperature ponds were dominated by archaea, mainly apparently novel representatives from the orders Sulfolobales and Thermoplasmatales that had no close cultivated relatives. By contrast, moderate temperature ponds were colonised by well-characterised sulphur-oxidising bacteria related to acidic environments, such as other geothermal sites or acid mine drainage, and archaea were absent. By combining the biodiversity results from this study and the reported physicochemical features of Copahue, a preliminary model of the possible biogeochemical interaction was outlined for moderate and high temperature ponds. Fil: Urbieta, María Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentina Fil: Gonzalez Toril, Elena. Consejo Superior de Investigaciones Cientificas; España Fil: Giaveno Filippa, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Comahue; Argentina Fil: Aguilera Bazán, A. Consejo Superior de Investigaciones Cientificas; España Fil: Donati, Edgardo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentina
- Published
- 2014
4. Draft Genome Sequence of the Novel Thermoacidophilic Archaeon Acidianus copahuensis Strain ALE1, Isolated from the Copahue Volcanic Area in Neuquén, Argentina
- Author
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Camila Castro, Martin P. Vazquez, Nicolás Rascovan, M. Alejandra Giaveno, M. Sofía Urbieta, Edgardo Ruben Donati, and Santiago Revale
- Subjects
Bioquímica ,Acidithiobacillus ,Sulfur oxidation ,Ciencias Biológicas ,purl.org/becyt/ford/1 [https] ,Biología Celular, Microbiología ,Extremophile ,Genetics ,Prokaryotes ,purl.org/becyt/ford/1.6 [https] ,Molecular Biology ,Gene ,Whole genome sequencing ,geography ,geography.geographical_feature_category ,Strain (chemistry) ,biology ,Copahue ,biology.organism_classification ,Archaea ,Volcano ,CIENCIAS NATURALES Y EXACTAS ,Sulfur ,Acidianus - Abstract
Acidianus copahuensis is a recently characterized thermoacidophilic archaeon isolated from the Copahue volcanic area in Argentina. Here, we present its draft genome sequence, in which we found genes involved in key metabolic pathways for developing under Copahue's extreme environmental conditions, such as sulfur and iron oxidation, carbon fixation, and metal tolerance., Centro de Investigación y Desarrollo en Fermentaciones Industriales
- Published
- 2014
5. Physiologic versatility and growth flexibility as the main characteristics of a novel thermoacidophilic Acidianus strain isolated from Copahue geothermal area in Argentina
- Author
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Elena González Toril, M. Alejandra Giaveno, J. Ricardo Ulloa, Edgardo Ruben Donati, and M. Sofía Urbieta
- Subjects
Acidianus infernus ,Iron ,Thirmophilic ,Argentina ,Soil Science ,Hot Springs ,Ciencias Biológicas ,chemistry.chemical_compound ,Biología Celular, Microbiología ,Bioleaching ,RNA, Ribosomal, 16S ,Botany ,Acidophilc ,Anaerobiosis ,Ecology, Evolution, Behavior and Systematics ,Phylogeny ,Tetrathionate ,Base Composition ,Ecology ,Strain (chemistry) ,biology ,Biodiversity ,Sequence Analysis, DNA ,biology.organism_classification ,16S ribosomal RNA ,Aerobiosis ,DNA, Archaeal ,chemistry ,Biochemistry ,Candidatus ,CIENCIAS NATURALES Y EXACTAS ,Acidianus ,Sulfur ,Archaea - Abstract
A novel thermoacidophilic archaeal strain has been isolated from three geothermal acidic hot springs in Copahue, Argentina. One of the most striking characteristic of ALE1 isolate is its metabolic versatility. It grows on sulphur, tetrathionate, iron (II) and sucrose under aerobic conditions, but it can also develop under anaerobic conditions using iron (III) or sulphur as electron acceptors and sulphur or hydrogen as electron donors autotrophically. A temperature of 75 °C and a pH between 2.5 and 3.0 are strain ALE1 optimal growth conditions, but it is able to oxidise iron (II) even at pH 1.0. Cells are irregular cocci surrounded by a regularly arrayed glycoprotein layer (S-layer). Phylogenetic analysis shows that strain ALE1 belongs to the family Sulfolobaceae in the class Thermoprotei, within the phylum Crenarchaeota. Based on 16S rRNA gene sequence similarity on NCBI database, ALE1 does not have closely related relatives, neither in culture nor uncultured, which is more surprising. Its closest related species are strains of Acidianus hospitalis (91 % of sequence similarity), Acidianus infernus (90 %), Acidianus ambivalens (90 %) and Acidianus manzanensis (90 %). Its DNA base composition of 34.5 %mol C+ G is higher than that reported for other Acidianus species. Considering physiological and phylogenetic characteristics of strain ALE1, we considered it to represent a novel species of the genus Acidianus (candidatus "Acidianus copahuensis"). The aim of this study is to physiologically characterise this novel archaea in order to understand its role in iron and sulphur geochemical cycles in the Copahue geothermal area and to evaluate its potential applications in bioleaching and biooxidation. Fil: Giaveno Filippa, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina Fil: Urbieta, María Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales (i); Argentina Fil: Ulloa, José Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina Fil: Toril, Elena. Instituto Nacional de Técnica Aeroespacial. Centro de Astrobiología; España Fil: Donati, Edgardo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
- Published
- 2012
6. First prokaryotic biodiversity assessment using molecular techniques of an acidic river in Neuquén, Argentina
- Author
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Angeles Aguilera, E. González Toril, M. Alejandra Giaveno, M. Sofía Urbieta, and Edgardo Ruben Donati
- Subjects
DNA, Bacterial ,Microorganism ,Molecular Sequence Data ,Argentina ,Soil Science ,Ferroplasma ,Water column ,Microbial ecology ,Rivers ,RNA, Ribosomal, 16S ,Ecology, Evolution, Behavior and Systematics ,Phylogeny ,Ecology ,biology ,Bacteria ,Thermophile ,Alicyclobacillus ,Acidithiobacillus thiooxidans ,Biodiversity ,Hydrogen-Ion Concentration ,biology.organism_classification ,Archaea ,Molecular Typing ,DNA, Archaeal ,Environmental chemistry - Abstract
Two acidic hot springs close to the crater of Copahue Volcano (Neuquen, Argentina) are the source of the Rio Agrio. The river runs several kilometres before flowing into Caviahue Lake. Along the river, temperature, iron, other metal and proton concentrations decrease gradually with distance downstream. From the source to the lake and depending on the season, pH can rise from 1.0 (or even less) to about 4.0, while temperature values decrease from 70°C to 15°C. Water samples were taken from different stations on the river selected according to their physicochemical parameters. In order to assess prokaryotic biodiversity throughout the water column, different and complementary molecular biology techniques were used, mainly in situ hybridisation and 16S rRNA gene cloning and sequencing. All microorganisms found are typical of acidic environments. Sulphur-oxidizing bacteria like Acidithiobacillus thiooxidans and Acidithiobacillus albertensis were detected in every station. Moderately thermophile iron- and sulphur-oxidizing bacteria like members of Alicyclobacillus and Sulfobacillus genera were also ubiquitous. Strict iron-oxidizing bacteria like Leptospirillum and Ferrimicrobium were present at the source of the river, but disappeared downstream where iron concentrations were much lower. Iron-oxidizing, mesophilic Ferroplasma spp. were the main archaea found. The data presented in this work represent the first molecular assessment of this rare natural acidic environment.
- Published
- 2011
7. Archaeal and bacterial diversity in five different hydrothermal ponds in the Copahue region in Argentina.
- Author
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Sofía Urbieta M, Toril EG, Alejandra Giaveno M, Bazán AA, and Donati ER
- Subjects
- Archaea genetics, Argentina, Bacteria genetics, Chemical Phenomena, Genes, Archaeal, Geography, Phylogeny, RNA, Ribosomal, 16S genetics, Archaea classification, Bacteria classification, Biodiversity, Hot Springs microbiology, Ponds microbiology, Water Microbiology
- Abstract
Copahue is an acidic geothermal volcanic region in the northwest corner of Neuquén Province, Argentina. In the area, there are various ponds, pools and hot springs with different temperatures, pH values and levels of anthropogenic influence. In this study, the prokaryotic biodiversity of five representative ponds was studied by using two complementary molecular ecology techniques: phylogenetic analysis of 16S rRNA bacterial and archaeal genes and FISH (or CARD-FISH) for quantitative estimation of biodiversity. The results, supported by multivariate statistical analysis, showed that the biodiversity in Copahue ponds seemed to be determined by temperature. High temperature ponds were dominated by archaea, mainly apparently novel representatives from the orders Sulfolobales and Thermoplasmatales that had no close cultivated relatives. By contrast, moderate temperature ponds were colonised by well-characterised sulphur-oxidising bacteria related to acidic environments, such as other geothermal sites or acid mine drainage, and archaea were absent. By combining the biodiversity results from this study and the reported physicochemical features of Copahue, a preliminary model of the possible biogeochemical interaction was outlined for moderate and high temperature ponds., (Copyright © 2014 Elsevier GmbH. All rights reserved.)
- Published
- 2014
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