1. Saccharomyces cerevisiae Mitoproteome Plasticity in Response to Recombinant Alternative Ubiquinol Oxidase
- Author
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Mathy, Grégory, Navet, Rachel, Gerkens, Pascal, Leprince, Pierre, De Pauw, Edwin, M. Sluse-Goffart, Claudine, E. Sluse, Francis, and Douette, Pierre
- Abstract
The energy-dissipating alternative oxidase (AOX) from Hansenula anomala was expressed in Saccharomyces cerevisiae. The recombinant AOX was functional. A comparative analysis by two-dimensional differential in-gel electrophoresis (2D-DIGE) of mitochondrial protein patterns found in wild-type and recombinant AOX strains was performed. 60 proteins exhibiting a significant difference in their abundance were identified. Interestingly, proteins implicated in major metabolic pathways such as Krebs cycle and amino acid biosynthesis were up-regulated. Surprisingly, an up-regulation of the respiratory-chain complex III was associated with a down-regulation of the ATP synthase complex. Keywords: alternative oxidase • yeast • mitoproteome • proteomics • two-dimensional differential in-gel electrophoresis
- Published
- 2006
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