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The meristem of three cultivars of Solanum tuberosum L., Ostara, Desirée and Eba was cultivated in vitro in order to observe the regeneration capacity and the organogenetic evolution of the tissues on various culture media. Meristems of 0.1-0.2 mm were prelevated from forced tubers; they were then inoculated on the different medium variants. (Table 1) The regeneration percentage for the in vitro meristem varied with the cultivar: 80 - 95% for Ostara, 70 – 80% for Desirée and 50 – 67% for Eba, as compared to 18 – 20% for the control medium (without hormones).The best variants proved to be M7 and M8 with all the cultivars, the regeneration percentage being the highest: Ostara 95%, Desirée 85% and Eba 67%. The in vitro organogenesis (number of stems, their height, number of roots and their length) was visible on all variants, according to the hormonal balance. The variants containing zeatine and 2-isopentenyl adenine (M5, M6, M7 şi M8) provided the best results. The Desiree had a special evolution, with about 45 neoplantlets /explant and almost double values in comparison with the other cultivars.

It’s emphasize the nonalternative character of Onobrychis sativa var. communis (Sparta sort), in spite of Splendid sort, bifera type, which presents alternativity; this one was obtain after a hybridation between four sainfoins, and this character probably is caused by the results of transgenic phenomenon responsible for this characters. Regarding of the dynamics of blooming is emphasize the precocious much more marked of the bifera type, of which the biological material, harvest from spontaneous flora, is closer and it exceed from this point of view communis type.

The perennial plants, included the sainfoin, make an extreme heterogenic group, characterized by an evident genetic variability, which offers multiples directions of achievement in improvement. In Onobrychis sativa there are two agriculture types, which correspond at botanical varieties bifera and communis, which distinguish in an obvious mode. The firs one, ssp. bifera, has the capacity of blooming from the first year of vegetation and after the successive mows from second year. The second type, ssp. Communis, is characterized by the fact that stays in vegetation in first year and after the first mow from the second year it has just vegetative offshoots. Improving programs from our country use just species with agriculture importance: Onobrychis sativa, Onobrychis sativa var. persica (sin. Onobrychis altissima), Onobrychis arenaria and Onobrychis transcaucasica.

IN VITRO MICROPROPAGATION OF SAINFOIN (ONOBRYCHIS VICIIFOLIA SCOP.) For the in vitro micropropagation there were used two types of explants, apexes and stem knots freshly inoculated. We took copse of different sizes, originating from plants situated in different years of vegetation (I, II, III). The incubated sainfoin knots and apexes were examined from the point of view of their in vitro behaviour regarding the regeneration, multiplication and striking root, depending on the hormonal balance from the culture medium and on the age of the donor plant. The viability and regeneration percentage of the sainfoin knot is comprised between 92 and 95 %, indifferently to the hormonal balance. The viability percentage of the apex is smaller than that of the knot, but its regeneration and multiplication capacity is higher. The regeneration degree is directly proportional with the age of the donor plant.