1. Autosomal SNP typing of forensic samples with the GenPlex™ HID System: results of a collaborative study
- Author
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Helle Smidt Mogensen, Antoinette A. Westen, F. Manohar, G. Axler-Diperte, C.R. Thacker, P. Hoff-Olsen, P. Kohler, S. Frisk Fredslund, M. L. Sonntag, Peter M. Schneider, Claus Børsting, K. Neureuther, Denise Syndercombe-Court, Olalla Maroñas, Anders J. Hansen, Cordula Haas, Melissa Scheible, Rixun Fang, Niels Morling, Z. M. Budimlija, Bertil Lindblom, Marcos F. Fondevila, Helena Nilsson, Amy E. Decker, Suzanne Gonzalez, Michael Stangegaard, Peter M. Vallone, Arthur J. Eisenberg, A. K. Kriegel, Michael D. Coble, Carmen Tomas, University of Zurich, and Tomas, C
- Subjects
Genetics ,Forensic Genetics ,340 Law ,Reproducibility of Results ,Single-nucleotide polymorphism ,Locus (genetics) ,610 Medicine & health ,Biology ,10218 Institute of Legal Medicine ,Polymorphism, Single Nucleotide ,Pathology and Forensic Medicine ,Discriminatory power ,Forensic science ,2734 Pathology and Forensic Medicine ,1311 Genetics ,SNP ,Humans ,Degraded dna ,Typing ,Cooperative Behavior ,Microsatellite Repeats - Abstract
The GenPlex (TM) HID System (Applied Biosystems - AB) offers typing of 48 of the 52 SNPforID SNPs and amelogenin. Previous studies have shown a high reproducibility of the GenPlex (TM) HID System using 250500 pg DNA of good quality. An international exercise was performed by 14 laboratories (9 in Europe and 5 in the US) in order to test the robustness and reliability of the GenPlex (TM) HID System on forensic samples. Three samples with partly degraded DNA and 10 samples with low amounts of DNA were analyzed in duplicates using various amounts of DNA. In order to compare the performance of the GenPlex (TM) HID System with the most commonly used STR kits, 500 pg of partly degraded DNA from three samples was typed by the laboratories using one or more STR kits. The median SNP typing success rate was 92.3% with 500 pg of partly degraded DNA. Three of the fourteen laboratories counted for more than two thirds of the locus dropouts. The median percentage of discrepant results was 0.2% with 500 pg degraded DNA. An increasing percentage of locus dropouts and discrepant results were observed when lower amounts of DNA were used. Different success rates were observed for the various SNPs. The rs763869 SNP was the least successful. With the exception of the MiniFiler (TM) kit (AB), GenPlex (TM) HID performed better than five other tested STR kits. When partly degraded DNA was analyzed, GenPlex (TM) HID showed a very low mean mach probability, while all STR kits except MiniFiler (TM) had very limited discriminatory power.
- Published
- 2010