1. Differential pre-mRNA Splicing Alters the Transcript Diversity of Helitrons Between the Maize Inbred Lines
- Author
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Tara L. Patrick, Amy E Siebert, Brian T. Lynch, Shailesh K. Lal, Katarina M. Klusman, Jennifer J. Moreno, Donya N. Shodja, and L. Curtis Hannah
- Subjects
0106 biological sciences ,Transposable element ,Molecular Sequence Data ,Biology ,Investigations ,01 natural sciences ,Plant Roots ,Zea mays ,03 medical and health sciences ,Exon ,Inbred strain ,Transcription (biology) ,Genetics ,RNA Precursors ,Molecular Biology ,Gene ,Genetics (clinical) ,030304 developmental biology ,2. Zero hunger ,0303 health sciences ,Base Sequence ,Helitron ,Alternative splicing ,transposable element ,Alternative Splicing ,RNA splicing ,gene evolution ,DNA Transposable Elements ,Genome, Plant ,Plant Shoots ,010606 plant biology & botany - Abstract
The propensity to capture and mobilize gene fragments by the highly abundant Helitron family of transposable elements likely impacts the evolution of genes in Zea mays. These elements provide a substrate for natural selection by giving birth to chimeric transcripts by intertwining exons of disparate genes. They also capture flanking exons by read-through transcription. Here, we describe the expression of selected Helitrons in different maize inbred lines. We recently reported that these Helitrons produce multiple isoforms of transcripts in inbred B73 via alternative splicing. Despite sharing high degrees of sequence similarity, the splicing profile of Helitrons differed among various maize inbred lines. The comparison of Helitron sequences identified unique polymorphisms in inbred B73, which potentially give rise to the alternatively spliced sites utilized by transcript isoforms. Some alterations in splicing, however, do not have obvious explanations. These observations not only add another level to the creation of transcript diversity by Helitrons among inbred lines but also provide novel insights into the cis-acting elements governing splice-site selection during pre-mRNA processing.
- Published
- 2015