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1. ICARUS at the Fermilab Short-Baseline Neutrino program: initial operation

Author: P. Abratenko, A. Aduszkiewicz, F. Akbar, M. Artero Pons, J. Asaadi, M. Aslin, M. Babicz, W. F. Badgett, L. F. Bagby, B. Baibussinov, B. Behera, V. Bellini, O. Beltramello, R. Benocci, J. Berger, S. Berkman, S. Bertolucci, R. Bertoni, M. Betancourt, M. Bettini, S. Biagi, K. Biery, O. Bitter, M. Bonesini, T. Boone, B. Bottino, A. Braggiotti, D. Brailsford, J. Bremer, S. J. Brice, V. Brio, C. Brizzolari, J. Brown, H. S. Budd, F. Calaon, A. Campani, D. Carber, M. Carneiro, I. Caro Terrazas, H. Carranza, D. Casazza, L. Castellani, A. Castro, S. Centro, G. Cerati, M. Chalifour, P. Chambouvet, A. Chatterjee, D. Cherdack, S. Cherubini, N. Chithirasreemadam, M. Cicerchia, V. Cicero, T. Coan, A. G. Cocco, M. R. Convery, S. Copello, E. Cristaldo, A. A. Dange, I. de Icaza Astiz, A. De Roeck, S. Di Domizio, L. Di Noto, C. Di Stefano, D. Di Ferdinando, M. Diwan, S. Dolan, L. Domine, S. Donati, R. Doubnik, F. Drielsma, J. Dyer, S. Dytman, C. Fabre, F. Fabris, A. Falcone, C. Farnese, A. Fava, H. Ferguson, A. Ferrari, F. Ferraro, N. Gallice, F. G. Garcia, M. Geynisman, M. Giarin, D. Gibin, S. G. Gigli, A. Gioiosa, W. Gu, M. Guerzoni, A. Guglielmi, G. Gurung, S. Hahn, K. Hardin, H. Hausner, A. Heggestuen, C. Hilgenberg, M. Hogan, B. Howard, R. Howell, J. Hrivnak, M. Iliescu, G. Ingratta, C. James, W. Jang, M. Jung, Y.-J. Jwa, L. Kashur, W. Ketchum, J. S. Kim, D.-H. Koh, U. Kose, J. Larkin, G. Laurenti, G. Lukhanin, S. Marchini, C. M. Marshall, S. Martynenko, N. Mauri, A. Mazzacane, K. S. McFarland, D. P. Méndez, A. Menegolli, G. Meng, O. G. Miranda, D. Mladenov, A. Mogan, N. Moggi, E. Montagna, C. Montanari, A. Montanari, M. Mooney, G. Moreno-Granados, J. Mueller, D. Naples, M. Nebot-Guinot, M. Nessi, T. Nichols, M. Nicoletto, B. Norris, S. Palestini, M. Pallavicini, V. Paolone, R. Papaleo, L. Pasqualini, L. Patrizii, R. Peghin, G. Petrillo, C. Petta, V. Pia, F. Pietropaolo, J. Poirot, F. Poppi, M. Pozzato, M. C. Prata, A. Prosser, G. Putnam, X. Qian, G. Rampazzo, A. Rappoldi, G. L. Raselli, R. Rechenmacher, F. Resnati, A. M. Ricci, G. Riccobene, L. Rice, E. Richards, A. Rigamonti, M. Rosenberg, M. Rossella, C. Rubbia, P. Sala, P. Sapienza, G. Savage, A. Scaramelli, A. Scarpelli, D. Schmitz, A. Schukraft, F. Sergiampietri, G. Sirri, J. S. Smedley, A. K. Soha, M. Spanu, L. Stanco, J. Stewart, N. B. Suarez, C. Sutera, H. A. Tanaka, M. Tenti, K. Terao, F. Terranova, V. Togo, D. Torretta, M. Torti, F. Tortorici, N. Tosi, Y.-T. Tsai, S. Tufanli, M. Turcato, T. Usher, F. Varanini, S. Ventura, F. Vercellati, M. Vicenzi, C. Vignoli, B. Viren, D. Warner, Z. Williams, R. J. Wilson, P. Wilson, J. Wolfs, T. Wongjirad, A. Wood, E. Worcester, M. Worcester, M. Wospakrik, H. Yu, J. Yu, A. Zani, P. G. Zatti, J. Zennamo, J. C. Zettlemoyer, C. Zhang, S. Zucchelli, and M. Zuckerbrot
Subjects: Astrophysics, QB460-466, Nuclear and particle physics. Atomic energy. Radioactivity, QC770-798
Abstract: Abstract The ICARUS collaboration employed the 760-ton T600 detector in a successful 3-year physics run at the underground LNGS laboratory, performing a sensitive search for LSND-like anomalous $$\nu _e$$ ν e appearance in the CERN Neutrino to Gran Sasso beam, which contributed to the constraints on the allowed neutrino oscillation parameters to a narrow region around 1 eV $$^2$$ 2 . After a significant overhaul at CERN, the T600 detector has been installed at Fermilab. In 2020 the cryogenic commissioning began with detector cool down, liquid argon filling and recirculation. ICARUS then started its operations collecting the first neutrino events from the booster neutrino beam (BNB) and the Neutrinos at the Main Injector (NuMI) beam off-axis, which were used to test the ICARUS event selection, reconstruction and analysis algorithms. ICARUS successfully completed its commissioning phase in June 2022. The first goal of the ICARUS data taking will be a study to either confirm or refute the claim by Neutrino-4 short-baseline reactor experiment. ICARUS will also perform measurement of neutrino cross sections with the NuMI beam and several Beyond Standard Model searches. After the first year of operations, ICARUS will search for evidence of sterile neutrinos jointly with the Short-Baseline Near Detector, within the Short-Baseline Neutrino program. In this paper, the main activities carried out during the overhauling and installation phases are highlighted. Preliminary technical results from the ICARUS commissioning data with the BNB and NuMI beams are presented both in terms of performance of all ICARUS subsystems and of capability to select and reconstruct neutrino events.
Published: 2023
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2. Overhaul and Installation of the ICARUS-T600 Liquid Argon TPC Electronics for the FNAL Short Baseline Neutrino Program

Author: A. Rappoldi, S. Ventura, Roberto Benocci, D. Torretta, Alessandro Menegolli, Angela Fava, C. Hilgenberg, S. Centro, Y. T. Tsai, Robert Wilson, Roberto Guida, Massimo Rossella, G. Moreno Granados, M. Mooney, R. Pedrotta, Marzio Nessi, A. Braggiotti, F. Fichera, G. Meng, M. Torti, David Warner, Gianluca Petrillo, F. Pietropaolo, M. Spanu, P.G. Zatti, F. Calaon, M. Nicoletto, V. Bellini, L. Castellani, Hs Budd, M. Giarin, James Mueller, B. Baibussinov, Carlo Rubbia, F. Vercellati, D. Gibin, Alfredo G. Cocco, M. Turcato, J. D. Brown, M. Betancourt, M. Worcester, A. Falcone, G. Rampazzo, B. Howard, C. Montanari, M. E. Convery, F. Varanini, G.L. Raselli, F. Tortorici, F. Sergiampietri, C. Vignoli, M. Bonesini, W. Ketchum, T. Boone, A. Guglielmi, A. Scaramelli, B. Behera, Christian Farnese, R. Peghin, M. Bettini, S. Marchini, F. Fabris, Andrea Zani, L. Bagby, Bagby, L, Baibussinov, B, Behera, B, Bellini, V, Benocci, R, Betancourt, M, Bettini, M, Bonesini, M, Boone, T, Braggiotti, A, Brown, J, Budd, H, Calaon, F, Castellani, L, Centro, S, Cocco, A, Convery, M, Fabris, F, Falcone, A, Farnese, C, Fava, A, Fichera, F, Giarin, M, Gibin, D, Guglielmi, A, Guida, R, Hilgenberg, C, Howard, B, Ketchum, W, Marchini, S, Menegolli, A, Meng, G, Montanari, C, Mooney, M, Granados, G, Mueller, J, Nessi, M, Nicoletto, M, Pedrotta, R, Peghin, R, Petrillo, G, Pietropaolo, F, Rampazzo, G, Rappoldi, A, Raselli, G, Rossella, M, Rubbia, C, Scaramelli, A, Sergiampietri, F, Spanu, M, Torretta, D, Torti, M, Tortorici, F, Tsai, Y, Turcato, M, Varanini, F, Ventura, S, Vercellati, F, Vignoli, C, Warner, D, Wilson, R, Worcester, M, Zani, A, and Zatti, P
Subjects: Physics - Instrumentation and Detectors, Nuclear engineering, Time projection chambers, Data acquisition circuits, FOS: Physical sciences, Flange, 01 natural sciences, 030218 nuclear medicine & medical imaging, High Energy Physics - Experiment, Neutrino detector, 03 medical and health sciences, High Energy Physics - Experiment (hep-ex), 0302 clinical medicine, Data acquisition circuit, Front-end electronics for detector readout, 0103 physical sciences, Electronics, Neutrino detectors, Detectors and Experimental Techniques, Instrumentation, physics.ins-det, Mathematical Physics, ICARUS, Physics, Time projection chamber, Large Hadron Collider, 010308 nuclear & particles physics, hep-ex, Detector, Instrumentation and Detectors (physics.ins-det), Upgrade, Neutrino, Particle Physics - Experiment
Abstract: The ICARUS T600 liquid argon (LAr) time projection chamber (TPC) underwent a major overhaul at CERN in 2016–2017 to prepare for the operation at FNAL in the Short Baseline Neutrino (SBN) program. This included a major upgrade of the photo-multiplier system and of the TPC wire read-out electronics. The full TPC wire read-out electronics together with the new wire biasing and interconnection scheme are described. The design of a new signal feed-through flange is also a fundamental piece of this overhaul whose major feature is the integration of all electronics components onto the signal flange. Initial functionality tests of the full TPC electronics chain installed in the T600 detector at FNAL are also described. The ICARUS T600 liquid argon (LAr) time projection chamber (TPC) underwent a major overhaul at CERN in 2016-2017 to prepare for the operation at FNAL in the Short Baseline Neutrino (SBN) program. This included a major upgrade of the photo-multiplier system and of the TPC wire read-out electronics. The full TPC wire read-out electronics together with the new wire biasing and interconnection scheme are described. The design of a new signal feed-through flange is also a fundamental piece of this overhaul whose major feature is the integration of all electronics components onto the signal flange. Initial functionality tests of the full TPC electronics chain installed in the T600 detector at FNAL are also described.
Published: 2020
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3. CMS technical design report, volume II: Physics performance

Author: G. L. BAYATIAN, S. CHATRCHYAN, G. HMAYAKYAN, A. M. SIRUNYAN, W. ADAM, T. BERGAUER, M. DRAGICEVIC, J. ERO, M. FRIEDL, R. FRUEHWIRTH, V. GHETE, P. GLASER, J. HRUBEC, M. JEITLER, M. KRAMMER, I. MAGRANS, I. MIKULEC, W. MITAROFF, T. NOEBAUER, M. PERNICKA, P. PORTH, H. ROHRINGER, J. STRAUSS, A. TAUROK, W. WALTENBERGER, G. WALZEL, E. WIDL, C. E. WULZ, A. FEDOROV, M. KORZHIK, O. MISSEVITCH, R. ZUYEUSKI, V. CHEKHOVSKY, O. DVORNIKOV, I. EMELIANTCHIK, A. LITOMIN, V. MOSSOLOV, N. SHUMEIKO, A. SOLIN, R. STEFANOVITCH, J. SUAREZ GONZALEZ, A. TIKHONOV, V. PETROV, J. D'HONDT, S. DE WEIRDT, R. GOORENS, J. HEYNINCK, S. LOWETTE, S. TAVERNIER, W. VAN DONINCK, L. VAN LANCKER, O. BOUHALI, B. CLERBAUX, G. DE LENTDECKER, J. P. DEWULF, T. MAHMOUD, P. E. MARAGE, L. NEUKERMANS, V. SUNDARARAJAN, C. VANDER VELDE, P. VANLAER, J. WICKENS, S. ASSOUAK, J. L. BONNET, G. BRUNO, J. CAUDRON, B. DE CALLATAY, J. DE FAVEREAU DE JENERET, S. DE VISSCHER, C. DELAERE, P. DEMIN, D. FAVART, E. FELTRIN, E. FORTON, G. GREGOIRE, S. KALININ, D. KCIRA, T. KEUTGEN, G. LEIBENGUTH, V. LEMAITRE, Y. LIU, D. MICHOTTE, O. MILITARU, A. NINANE, S. OVYN, T. PIERZCHALA, K. PIOTRZKOWSKI, V. ROBERFROID, X. ROUBY, D. TEYSSIER, O. VAN DER AA, M. VANDER DONCKT, E. DAUBIE, P. HERQUET, A. MOLLET, A. ROMEYER, W. BEAUMONT, M. CARDACI, E. DE LANGHE, E. A. DE WOLF, L. RURUA, M. H. G. SOUZA, V. OGURI, A. SANTORO, A. SZNAJDER, M. VAZ, E. M. GREGORES, S. F. NOVAES, T. ANGUELOV, G. ANTCHEV, I. ATANASOV, J. DAMGOV, N. DARMENOV, L. DIMITROV, V. GENCHEV, P. IAYDJIEV, B. PANEV, S. PIPEROV, S. STOYKOVA, G. SULTANOV, I. VANKOV, A. DIMITROV, V. KOZHUHAROV, L. LITOV, M. MAKARIEV, A. MARINOV, E. MARINOVA, S. MARKOV, M. MATEEV, B. PAVLOV, P. PETKOV, C. SABEV, S. STOYNEV, Z. TOTEVA, V. VERGUILOV, G. M. CHEN, H. S. CHEN, K. L. HE, C. H. JIANG, W. G. LI, H. M. LIU, X. MENG, X. Y. SHEN, H. S. SUN, M. YANG, W. R. ZHAO, H. L. ZHUANG, Y. BAN, J. CAI, S. LIU, S. J. QIAN, Z. C. YANG, Y. L. YE, J. YING, J. WU, Z. P. ZHANG, N. GODINOVIC, I. PULJAK, I. SORIC, Z. ANTUNOVIC, M. DZELALIJA, K. MARASOVIC, V. BRIGLJEVIC, D. FERENCEK, K. KADIJA, S. MOROVIC, M. PLANINIC, C. NICOLAOU, A. PAPADAKIS, P. A. RAZIS, D. TSIAKKOURI, A. HEKTOR, M. KADASTIK, K. KANNIKE, E. LIPPMAA, M. MUNTEL, M. RAIDAL, P. A. AARNIO, S. CZELLAR, E. HAEGGSTROEM, A. HEIKKINEN, J. HARKONEN, V. KARIMAKI, R. KINNUNEN, T. LAMPEN, K. LASSILA PERINI, S. LEHTI, T. LINDEN, P. R. LUUKKA, S. MICHAL, T. MAENPAA, J. NYSTEN, M. STETTLER, E. TUOMINEN, J. TUOMINIEMI, L. WENDLAND, T. TUUVA, J. P. GUILLAUD, P. NEDELEC, D. SILLOU, M. ANFREVILLE, S. BEAUCERON, E. BOUGAMONT, P. BREDY, R. CHIPAUX, M. DEJARDIN, D. DENEGRI, J. DESCAMPS, B. FABBRO, J. L. FAURE, S. GANJOUR, F. X. GENTIT, A. GIVERNAUD, P. GRAS, G. HAMEL DE MONCHENAULT, P. JARRY, F. KIRCHER, M. C. LEMAIRE, B. LEVESY, E. LOCCI, J. P. LOTTIN, I. MANDJAVIDZE, M. MUR, E. PASQUETTO, A. PAYN, J. RANDER, J. M. REYMOND, F. RONDEAUX, A. ROSOWSKY, Z. H. SUN, P. VERRECCHIA, S. BAFFIONI, F. BEAUDETTE, M. BERCHER, U. BERTHON, S. BIMBOT, J. BOUROTTE, P. BUSSON, M. CERUTTI, D. CHAMONT, C. CHARLOT, C. COLLARD, D. DECOTIGNY, E. DELMEIRE, L. DOBRZYNSKI, A. M. GAILLAC, Y. GEEREBAERT, J. GILLY, M. HAGUENAUER, A. KARAR, A. MATHIEU, G. MILLERET, P. MINE, P. PAGANINI, T. ROMANTEAU, I. SEMENIOUK, Y. SIROIS, J. D. BERST, J. M. BROM, F. DIDIERJEAN, F. DROUHIN, J. C. FONTAINE, U. GOERLACH, P. GRAEHLING, L. GROSS, L. HOUCHU, P. JUILLOT, A. LOUNIS, C. MAAZOUZI, D. MANGEOL, C. OLIVETTO, T. TODOROV, P. VAN HOVE, D. VINTACHE, M. AGERON, J. L. AGRAM, G. BAULIEU, M. BEDJIDIAN, J. BLAHA, A. BONNEVAUX, G. BOUDOUL, E. CHABANAT, C. COMBARET, D. CONTARDO, R. DELLA NEGRA, P. DEPASSE, T. DUPASQUIER, H. EL MAMOUNI, N. ESTRE, J. FAY, S. GASCON, N. GIRAUD, C. GIRERD, R. HAROUTUNIAN, J. C. IANIGRO, B. ILLE, M. LETHUILLIER, N. LUMB, H. MATHEZ, G. MAURELLI, L. MIRABITO, S. PERRIES, O. RAVAT, R. KVATADZE, V. ROINISHVILI, R. ADOLPHI, R. BRAUER, W. BRAUNSCHWEIG, H. ESSER, L. FELD, A. HEISTER, W. KARPINSKI, K. KLEIN, C. KUKULIES, J. OLZEM, A. OSTAPCHUK, D. PANDOULAS, G. PIERSCHEL, F. RAUPACH, S. SCHAEL, G. SCHWERING, M. THOMAS, M. WEBER, B. WITTMER, M. WLOCHAL, A. ADOLF, P. BIALLASS, M. BONTENACKELS, M. ERDMANN, H. FESEFELDT, T. HEBBEKER, S. HERMANN, G. HILGERS, K. HOEPFNER, C. HOF, S. KAPPLER, M. KIRSCH, D. LANSKE, B. PHILIPPS, H. REITHLER, T. ROMMERSKIRCHEN, M. SOWA, H. SZCZESNY, M. TONUTTI, O. TSIGENOV, F. BEISSEL, M. DAVIDS, M. DUDA, G. FLUGGE, T. FRANKE, M. GIFFELS, T. HERMANNS, D. HEYDHAUSEN, S. KASSELMANN, G. KAUSSEN, T. KRESS, A. LINN, A. NOWACK, M. POETTGENS, O. POOTH, A. STAHL, D. TORNIER, A. FLOSSDORF, B. HEGNER, J. MNICH, C. ROSEMANN, G. FLUCKE, U. HOLM, R. KLANNER, U. PEIN, N. SCHIRM, P. SCHLEPER, G. STEINBRUCK, M. STOYE, R. VAN STAA, K. WICK, P. BLUM, V. BUEGE, W. DE BOER, G. DIRKES, M. FAHRER, M. FEINDT, U. FELZMANN, J. FERNANDEZ MENENDEZ, M. FREY, A. FURGERI, F. HARTMANN, S. HEIER, C. JUNG, B. LEDERMANN, T.H. MULLER, M. NIEGEL, A. OEHLER, T. ORTEGA GOMEZ, C. PIASECKI, G. QUAST, K. RABBERTZ, C. SAOUT, A. SCHEURER, D. SCHIEFERDECKER, A. SCHMIDT, H. J. SIMONIS, A. THEEL, A. VEST, T. WEILER, C. WEISER, J. WENG, V. ZHUKOV, G. KARAPOSTOLI, P. KATSAS, P. KREUZER, A. PANAGIOTOU, C. PAPADIMITROPOULOS, G. ANAGNOSTOU, M. BARONE, T. GERALIS, C. KALFAS, A. KOIMAS, A. KYRIAKIS, S. KYRIAZOPOULOU, D. LOUKAS, A. MARKOU, C. MARKOU, C. MAVROMMATIS, K. THEOFILATOS, G. VERMISOGLOU, A. ZACHARIADOU, X. ASLANOGLOU, I. EVANGELOU, P. KOKKAS, N. MANTHOS, I. PAPADOPOULOS, G. SIDIROPOULOS, F. A. TRIANTIS, G. BENCZE, L. BOLDIZSAR, C. HAJDU, D. HORVATH, A. LASZLO, G. ODOR, F. SIKLER, N. TOTH, G. VESZTERGOMBI, P. ZALAN, J. MOLNAR, N. BENI, A. KAPUSI, G. MARIAN, P. RAICS, Z. SZABO, Z. SZILLASI, G. ZILIZ, H. S. BAWA, S. B. BERI, V. BHANDARI, V. BHATNAGAR, M. KAUR, R. KAUR, J. M. KOHLI, A. KUMAR, J. B. SINGH, A. BHARDWAJ, S. BHATTACHARYA, S. CHATTERJI, S. CHAUHAN, B. C. CHOUDHARY, P. GUPTA, M. JHA, K. RANJAN, R. K. SHIVPURI, A. K. SRIVASTAVA, S. BORKAR, M. DIXIT, M. GHODGAONKAR, S. K. KATARIA, S. K. LALWANI, V. MISHRA, A. K. MOHANTY, A. TOPKAR, T. AZIZ, S. BANERJEE, S. BOSE, N. CHEERE, S. CHENDVANKAR, P. V. DESHPANDE, M. GUCHAIT, A. GURTU, M. MAITY, G. MAJUMDER, K. MAZUMDAR, A. NAYAK, M. R. PATIL, S. SHARMA, K. SUDHAKAR, S. C. TONWAR, B. S. ACHARYA, S. BHEESETTE, S. DUGAD, S. D. KALMANI, V. R. LAKKIREDDI, N. K. MONDAL, N. PANYAM, P. VERMA, M. ARABGOL, H. ARFAEI, M. HASHEMI, M. MOHAMMADI, M. MOHAMMADI NAJAFABADI, A. MOSHAII, S. PAKTINAT MEHDIABADI, M. GRUNEWALD, M. ABBRESCIA, L. BARBONE, A. COLALEO, D. CREANZA, N. DE FILIPPIS, M. DE PALMA, G. DONVITO, L. FIORE, D. GIORDANO, G. IASELLI, F. LODDO, G. MAGGI, M. MAGGI, N. MANNA, B. MARANGELLI, M. S. MENNEA, S. MY, S. NATALI, S. NUZZO, G. PUGLIESE, V. RADICCI, A. RANIERI, F. ROMANO, G. SELVAGGI, L. SILVESTRIS, P. TEMPESTA, R. TRENTADUE, G. ZITO, G. ABBIENDI, W. BACCHI, A. BENVENUTI, S. BRAIBANT GIACOMELLI, F. R. CAVALLO, C. CIOCCA, G. CODISPOTI, I. D'ANTONE, G. M. DALLAVALLE, P. GIACOMELLI, C. GRANDI, M. GUERZONI, S. MARCELLINI, F. ODORICI, S. ALBERGO, M. CHIORBOLI, S. COSTA, M. GALANTI, G. GATTO ROTONDO, F. NOTO, R. POTENZA, G. RUSSO, A. TRICOMI, C. TUVE, A. BOCCI, G. CIRAOLO, V. CIULLI, C. CIVININI, R. D'ALESSANDRO, E. FOCARDI, C. GENTA, P. LENZI, A. MACCHIOLO, N. MAGINI, F. MANOLESCU, C. MARCHETTINI, L. MASETTI, S. MERSI, M. MESCHINI, S. PAOLETTI, G. PARRINI, R. RANIERI, M. SANI, P. FABBRICATORE, S. FARINON, M. GRECO, G. CATTANEO, A. DE MIN, M. DOMINONI, F. M. FARINA, F. FERRI, A. GHEZZI, P. GOVONI, R. LEPORINI, S. MAGNI, M. MALBERTI, S. MALVEZZI, S. MARELLI, D. MENASCE, L. MORONI, P. NEGRI, M. PAGANONI, D. PEDRINI, A. PULLIA, S. RAGAZZI, N. REDAELLI, C. ROVELLI, M. ROVERE, L. SALA, S. SALA, R. SALERNO, T. TABARELLI DE FATIS, S. VIGANO, G. COMUNALE, F. FABOZZI, D. LOMIDZE, S. MELE, P. PAOLUCCI, D. PICCOLO, G. POLESE, C. SCIACCA, P. AZZI, N. BACCHETTA, M. BELLATO, M. BENETTONI, D. BISELLO, E. BORSATO, A. CANDELORI, P. CHECCHIA, E. CONTI, M. DE MATTIA, T. DORIGO, V. DROLLINGER, F. FANZAGO, F. GASPARINI, U. GASPARINI, M. GIARIN, P. GIUBILATO, F. GONELLA, A. KAMINSKIY, S. KARAEVSKII, V. KHOMENKOV, S. LACAPRARA, I. LIPPI, M. LORETI, O. LYTOVCHENKO, M. MAZZUCATO, A. T. MENEGUZZO, M. MICHELOTTO, F. MONTECASSIANO, M. NIGRO, M. PASSASEO, M. PEGORARO, G. RAMPAZZO, P. RONCHESE, E. TORASSA, S. VENTURA, M. ZANETTI, P. ZOTTO, G. ZUMERLE, G. BELLI, U. BERZANO, C. DE VECCHI, R. GUIDA, M. M. NECCHI, S. P. RATTI, C. RICCARDI, G. SANI, P. TORRE, P. VITULO, F. AMBROGLINI, E. BABUCCI, D. BENEDETTI, M. BIASINI, G. M. BILEI, B. CAPONERI, B. CHECCUCCI, L. FANO, P. LARICCIA, G. MANTOVANI, D. PASSERI, M. PIOPPI, P. PLACIDI, V. POSTOLACHE, D. RICCI, A. SANTOCCHIA, L. SERVOLI, D. SPIGA, P. AZZURRI, GIUSEPPE BAGLIESI, A. BASTI, L. BENUCCI, J. BERNARDINI, T. BOCCALI, L. BORRELLO, F. BOSI, F. CALZOLARI, R. CASTALDI, C. CERRI, A. S. CUCOANES, M. D'ALFONSO, R. DELL'ORSO, S. DUTTA, L. FOA, S. GENNAI, A. GIAMMANCO, A. GIASSI, D. KARTASHOV, F. LIGABUE, S. LINARI, T. LOMTADZE, G. A. LUNGU, B. MANGANO, G. MARTINELLI, M. MASSA, A. 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ALEXANDER, D. CASSEL, K. ECKLUND, B. HELTSLEY, C.D. JONES, V. KUZNETSOV, J.R. PATTERSON, A. RYD, J. THOM, P. WITTICH, C.P. BEETZ, G. CIRINO, V. PODRASKY, C. SANZENI, D. WINN, S. ABDULLIN, M.A. AFAQ, M. ALBROW, J. AMUNDSON, G. APOLLINARI, M. ATAC, W. BADGETT, J.A. BAKKEN, B. BALDIN, L.A.T. BAUERDICK, A. BAUMBAUGH, U. BAUR, P.C. BHAT, F. BORCHERDING, K. BURKETT, J.N. BUTLER, H. CHEUNG, I. CHURIN, S. CIHANGIR, M. DEMARTEAU, D.P. EARTLY, J.E. ELIAS, V.D. ELVIRA, I. FISK, J. FREEMAN, P. GARTUNG, F.J.M. GEURTS, D.A. GLENZINSKI, E. GOTTSCHALK, G. GRAHAM, D. GREEN, G.M. GUGLIELMO, Y. GUO, O. GUTSCHE, A. HAHN, J. HANLON, S. HANSEN, R.M. HARRIS, T. HESSELROTH, S.L. HOLM, B. HOLZMAN, S. IQBAL, E. JAMES, M. JOHNSON, U. JOSHI, B. KLIMA, J. KOWALKOWSKI, T. KRAMER, S. KWAN, E. LA VALLIE, M. LARWILL, S. LOS, L. LUEKING, G. LUKHANIN, S. LUSIN, K. MAESHIMA, P. MCBRIDE, S.J. MURRAY, V. O'DELL, M. PATERNO, J. PATRICK, D. PETRAVICK, R. PORDES, O. PROKOFYEV, V. RASMISLOVICH, N. RATNIKOVA, A. RONZHIN, V. SEKHRI, E. SEXTON-KENNEDY, T. SHAW, D. SKOW, R.P. SMITH, W.J. SPALDING, L. SPIEGEL, M. STAVRIANAKOU, G. STIEHR, I. SUZUKI, P. TAN, W. TANENBAUM, S. TKACZYK, S. VESELI, R. VIDAL, H. WENZEL, J. WHITMORE, W.J. WOMERSLEY, W.M. WU, Y. WU, A. YAGIL, J. YARBA, J.C. YUN, D. ACOSTA, P. AVERY, V. BARASHKO, P. BARTALINI, D. BOURILKOV, R. CAVANAUGH, A. DROZDETSKIY, R.D. FIELD, Y. FU, L. GRAY, D. HOLMES, B.J. KIM, S. KLIMENKO, J. KONIGSBERG, A. KORYTOV, K. KOTOV, P. LEVCHENKO, A. MADORSKY, K. MATCHEV, GUENAKH MITSELMAKHER, Y. PAKHOTIN, C. PRESCOTT, P. RAMOND, J.L. RODRIGUEZ, M. SCHMITT, B. SCURLOCK, H. STOECK, J. YELTON, W. BOEGLIN, V. GAULTNEY, L. KRAMER, S. LINN, P. MARKOWITZ, G. MARTINEZ, B. RAUE, J. REINHOLD, A. ASKEW, M. BERTOLDI, W.G.D. DHARMARATNA, Y. GERSHTEIN, S. HAGOPIAN, V. HAGOPIAN, M. JENKINS, K.F. JOHNSON, H. PROSPER, H. WAHL, M. BAARMAND, L. BAKSAY, S. GURAGAIN, M. HOHLMANN, H. MERMERKAYA, R. RALICH, I. VODOPIYANOV, M.R. ADAMS, R.R. BETTS, C.E. GERBER, E. SHABALINA, C. SMITH, T. TEN, U. AKGUN, A.S. AYAN, A. COOPER, P. DEBBINS, F. DURU, M. FOUNTAIN, N. GEORGE, E. MCCLIMENT, J.P. MERLO, A. MESTVIRISHVILI, M.J. MILLER, C.R. NEWSOM, E. NORBECK, Y. ONEL, I. SCHMIDT, S. WANG, E.W. ANDERSON, O. ATRAMENTOV, J.M. HAUPTMAN, J. LAMSA, B.A. BARNETT, B. BLUMENFELD, C.Y. CHIEN, D.W. KIM, P. MAKSIMOVIC, S. SPANGLER, M. SWARTZ, P. BARINGER, A. BEAN, D. COPPAGE, O. GRACHOV, E.J. KIM, M. MURRAY, D. BANDURIN, T. BOLTON, A. KHANOV, Y. MARAVIN, D. ONOPRIENKO, F. RIZATDINOVA, R. SIDWELL, N. STANTON, E. VON TOERNE, D. BADEN, R. BARD, S.C. ENO, T. GRASSI, N.J. HADLEY, R.G. KELLOGG, S. KUNORI, F. RATNIKOV, A. SKUJA, R. ARCIDIACONO, M. BALLINTIJN, G. BAUER, P. HARRIS, I. KRAVCHENKO, C. LOIZIDES, S. NAHN, C. PAUS, S. PAVLON, C. ROLAND, G. ROLAND, K. SUMOROK, S. VAURYNOVICH, G. VERES, B. WYSLOUCH, D. BAILLEUX, S. CORUM, P. CUSHMAN, A. DE BENEDETTI, A. DOLGOPOLOV, R. EGELAND, G. FRANZONI, W.J. GILBERT, J. GRAHL, J. HAUPT, Y. KUBOTA, J. MANS, N. PEARSON, R. RUSACK, A. SINGOVSKY, L.M. CREMALDI, R. GODANG, R. KROEGER, D.A. SANDERS, D. SUMMERS, K. BLOOM, D.R. CLAES, A. DOMINGUEZ, M. EADS, C. LUNDSTEDT, S. MALIK, G.R. SNOW, A. SOBOL, I. IASHVILI, A. KHARCHILAVA, G. ALVERSON, E. BARBERIS, O. BOERIU, G. EULISSE, Y. MUSIENKO, S. MUZAFFAR, I. OSBORNE, S. REUCROFT, J. SWAIN, L. TAYLOR, L. TUURA, D. WOOD, B. GOBBI, M. KUBANTSEV, H. SCHELLMAN, E. SPENCER, M. VELASCO, B. BAUMBAUGH, N.M. CASON, M. HILDRETH, D.J. KARMGARD, N. MARINELLI, R. RUCHTI, J. WARCHOL, M. WAYNE, B. BYLSMA, L.S. DURKIN, J. GILMORE, J. GU, D. HERMAN, P. KILLEWALD, K. KNOBBE, T.Y. LING, P. ELMER, D. MARLOW, P. PIROUE, D. STICKLAND, C. TULLY, T. WILDISH, S. WYNHOFF, Z. XIE, A. APRESYAN, K. ARNDT, K. BANICZ, V.E. BARNES, G. BOLLA, D. BORTOLETTO, A. BUJAK, A.F. GARFINKEL, O. GONZALEZ LOPEZ, L. GUTAY, N. IPPOLITO, Y. KOZHEVNIKOV, A.T. LAASANEN, C. LIU, V. MAROUSSOV, P. MERKEL, D.H. MILLER, J. MIYAMOTO, N. NEUMEISTER, C. ROTT, A. ROY, A. SEDOV, I. SHIPSEY, N. PARASHAR, G. EPPLEY, S.J. LEE, J. LIU, M. MATVEEV, T. NUSSBAUM, B.P. PADLEY, J. ROBERTS, A. TUMANOV, P. YEPES, A. BODEK, H. BUDD, Y.S. CHUNG, P. DE BARBARO, R. DEMINA, R. EUSEBI, G. GINTHER, Y. GOTRA, A. HOCKER, U. HUSEMANN, S. KORJENEVSKI, W. SAKUMOTO, P. SLATTERY, P. TIPTON, M. ZIELINSKI, E. BARTZ, J. DOROSHENKO, E. HALKIADAKIS, P.F. JACQUES, M.S. KALELKAR, D. KHITS, A. LATH, A. MACPHERSON, L. PERERA, R. PLANO, K. ROSE, S. SCHNETZER, S. SOMALWAR, R. STONE, G. THOMSON, T.L. WATTS, N. AKCHURIN, K.W. CARRELL, K. GUMUS, C. JEONG, H. KIM, V. PAPADIMITRIOU, A. SILL, M. SPEZZIGA, E. WASHINGTON, R. WIGMANS, L. ZHANG, T. BAPTY, D. ENGH, W. JOHNS, T. KESKINPALA, E. LUIGGI LOPEZ, S. NEEMA, S. NORDSTROM, S. PATHAK, P. SHELDON, E.W. VAANDERING, M. WEBSTER, M.W. ARENTON, S. CONETTI, B. COX, R. HIROSKY, R. IMLAY, A. LEDOVSKOY, D. PHILLIPS, H. POWELL, M. RONQUEST, D. SMITH, Y.W. BAEK, J.N. BELLINGER, D. BRADLEY, D. CARLSMITH, I. CROTTY, S. DASU, F. FEYZI, T. GORSKI, M. GROTHE, W. HOGG, M. JAWORSKI, P. KLABBERS, A. LANARO, R. LOVELESS, M. MAGRANS DE ABRIL, D. REEDER, W.H. SMITH, D. WENMAN, G.S. ATOYAN, S. DHAWAN, V. ISSAKOV, H. NEAL, A. POBLAGUEV, M.E. ZELLER, and B.S. YULDASHEV
Subjects: High Energy Physics::Phenomenology, STANDARD MODEL, High Energy Physics::Experiment, EXTRA DIMENSIONS, LHC, SUPERSYMMETRY, HIGGS
Abstract: CMS is a general purpose experiment, designed to study the physics of pp collisions at 14 TeV at the Large Hadron Collider (LHC). It currently involves more than 2000 physicists from more than 150 institutes and 37 countries. The LHC will provide extraordinary opportunities for particle physics based on its unprecedented collision energy and luminosity when it begins operation in 2007. The principal aim of this report is to present the strategy of CMS to explore the rich physics programme offered by the LHC. This volume demonstrates the physics capability of the CMS experiment. The prime goals of CMS are to explore physics at the TeV scale and to study the mechanism of electroweak symmetry breaking—through the discovery of the Higgs particle or otherwise. To carry out this task, CMS must be prepared to search for new particles, such as the Higgs boson or supersymmetric partners of the Standard Model particles, from the start-up of the LHC since new physics at the TeV scale may manifest itself with modest data samples of the order of a few fb-1 or less. The analysis tools that have been developed are applied to study in great detail and with all the methodology of performing an analysis on CMS data specific benchmark processes upon which to gauge the performance of CMS. These processes cover several Higgs boson decay channels, the production and decay of new particles such as Z' and supersymmetric particles, Bs production and processes in heavy ion collisions. The simulation of these benchmark processes includes subtle effects such as possible detector miscalibration and misalignment. Besides these benchmark processes, the physics reach of CMS is studied for a large number of signatures arising in the Standard Model and also in theories beyond the Standard Model for integrated luminosities ranging from 1 fb-1 to 30 fb-1. The Standard Model processes include QCD, B-physics, diffraction, detailed studies of the top quark properties, and electroweak physics topics such as the W and Z0 boson properties. The production and decay of the Higgs particle is studied for many observable decays, and the precision with which the Higgs boson properties can be derived is determined. About ten different supersymmetry benchmark points are analysed using full simulation. The CMS discovery reach is evaluated in the SUSY parameter space covering a large variety of decay signatures. Furthermore, the discovery reach for a plethora of alternative models for new physics is explored, notably extra dimensions, new vector boson high mass states, little Higgs models, technicolour and others. Methods to discriminate between models have been investigated. This report is organized as follows. Chapter 1, the Introduction, describes the context of this document. Chapters 2–6 describe examples of full analyses, with photons, electrons, muons, jets, missing ET, B-mesons and τ's, and for quarkonia in heavy ion collisions. Chapters 7–15 describe the physics reach for Standard Model processes, Higgs discovery and searches for new physics beyond the Standard Model.
Published: 2007

4. Quantitative analysis of wild-type and HBeAg minus hepatitis B viruses by a sequence-dependent primer extension assay

Author: M. Giarin, Filippo Oliveri, A Jalanko, Marjut Ranki, Gabriella Capra, Maurizia Rossana Brunetto, Pl Calvo, Ferruccio Bonino, Piantino P, and A Randone
Subjects: Hepatitis B virus, HBsAg, Genes, Viral, DNA Mutational Analysis, Molecular Sequence Data, Viremia, medicine.disease_cause, Polymerase Chain Reaction, Virus, Primer extension, Virology, medicine, Humans, Point Mutation, Hepatitis B e Antigens, DNA Primers, Hepatitis, Chronic, Base Sequence, biology, virus diseases, Hepatitis B, biology.organism_classification, medicine.disease, Molecular biology, digestive system diseases, Infectious Diseases, Genetic Techniques, Hepadnaviridae, HBeAg, DNA, Viral
Abstract: The ratio between wild-type hepatitis B virus (HBV) and HBV mutant, unable to secrete “e” antigen (HBeAg minus HBV) appears to be an important determinant of the outcome of chronic hepatitis B. Quantitative analysis of wild-type and HBeAg minus HBVs in the blood could be useful to monitor chronic hepatitis B patients. We developed a solid-phase minisequencing assay for both viruses using a primer-guided incorporation of a single labeled nucleotide on an affinity captured biotinylated amplified HBV-DNA template. A standard curve was constructed by mixing increasing quantities of wild type and mutant virus DNAs. The detection of wild-type and HBeAg minus sequences, ranging from 10% to 90% of overall viremia, was linear and reproducible till 0.1 pg/μl of serum HBV-DNA. The assay yields numerical values and the ratio of incorporated nucleotides defines the relative proportions (%) of the two viral sequences with accuracy. We tested the sensitivity and accuracy of the minisequencing on mixed end point dilutions of wild-type and HBeAg minus reference sera and amplified products. The feasibility and reproducibility of the assay were tested in 35 sera from 21 HBsAg positive patients with chronic hepatitis B using both minisequencing and oligo-hybridization assays. A high correlation was found between the two assays (r = 0.957 P < 0.0001). In conclusion, the minisequencing assay provides a precise and reproducible quantitative analysis of wild-type and HBeAg minus HBVs in clinical specimens. It is proposed to study the relations between HBV heterogeneity and the course of hepatitis B and its response to therapy. © 1994 Wiley-Liss, Inc.
Published: 1994
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5. Serum free light chain ratio, total kappa/lambda ratio, and immunofixation results are not prognostic factors after stem cell transplantation for newly diagnosed multiple myeloma

Author: Barbara Amoroso, Mario Boccadoro, Manuela M. Giarin, Alessia Cipriani, Luisa Giaccone, Federica Cavallo, Antonio Palumbo, Roberto Sorasio, and C. Sfiligoi
Subjects: Immunofixation, Adult, Male, medicine.medical_specialty, Pathology, Clinical Biochemistry, Kaplan-Meier Estimate, Gastroenterology, Transplantation, Autologous, Disease-Free Survival, Cohort Studies, Immunoglobulin kappa-Chains, Immunoglobulin lambda-Chains, hemic and lymphatic diseases, Internal medicine, Immunopathology, medicine, Humans, Multiple myeloma, Aged, Retrospective Studies, biology, business.industry, Biochemistry (medical), Cancer, Retrospective cohort study, Middle Aged, medicine.disease, Prognosis, Transplantation, Cohort, biology.protein, Female, Stem cell, business, Multiple Myeloma, Stem Cell Transplantation
Abstract: Background: The prognostic value of changes in paraprotein markers after stem cell transplantation is unknown. We evaluated disease response using serum immunofixation (s-IFIX), total κ and λ ratio (KLR), and free light chain (FLC) ratio in myeloma patients who underwent autologous or autologous plus allogeneic stem cell transplantation. Methods: We studied s-IFIX, KLR, and FLC ratio in sera from 203 patients, 3 months after transplantation. We evaluated overall and event-free survival (OS and EFS, interval between date of study enrollment and date of death from any cause or date of progression, relapse, or death from any cause, respectively) by the Kaplan–Meier method. Results: Of the 203 patients, 51 were negative by s-IFIX, 99 reached a normal KLR, and 92 had a normal FLC ratio. Of the 51 patients with negative s-IFIX, 40 (78%) also had a normal FLC ratio. The median duration of OS was 54.3 months, and the median EFS was 19.5 months. None of the measured paraprotein parameters showed an association with OS. Only a normal KLR was associated with prolonged EFS (P = 0.016). Even a negative s-IFIX associated with a normal FLC ratio did not show a significant difference in terms of EFS and OS. Conclusions: Our analysis with a small cohort of patients did not show a significant impact of achieving complete response (CR) or stringent CR on patient survival.
Published: 2009

6. ‘e’ Antigen defective hepatitis B virus and course of chronic infection

Author: M.L. Abate, Giorgio Maria Saracco, Cristiana Barbera, Giorgio Verme, M. Giarin, E. Chiaberge, Filippo Oliveri, Maurizia Rossana Brunetto, Ferruccio Bonino, Pier Luigi Calvo, Paola Manzini, and T. Parrella
Subjects: Adult, Male, Molecular Sequence Data, medicine.disease_cause, Virus, Serology, Liver disease, Antigen, medicine, Humans, Hepatitis B e Antigens, Viremia, Child, Hepatitis, Hepatitis B virus, Hepatitis B Surface Antigens, Base Sequence, Hepatology, business.industry, Age Factors, Defective Viruses, virus diseases, Hepatitis B, medicine.disease, Virology, digestive system diseases, Chronic infection, Immunoglobulin M, Oligodeoxyribonucleotides, HBeAg, Carrier State, Immunology, Female, business, Follow-Up Studies
Abstract: We studied the relations between HBV heterogeneity and different phases of HBV infection and disease in 145 HBsAg-positive carriers followed-up for 28 months (range 24–60 months). Viraemia was characterized for the relative prevalence of wild-type and HBeAg minus HBVs after HBV-DNA amplification by PCR using an oligonucleotide hybridization assay. HBeAg minus HBV was detected in 27% of immunotolerant HBV carriers, in 67% of patients with chronic hepatitis B (immunoelimination phase) and in 17% of HBsAg carriers with latent infection. Serum HBV-DNA and IgM anti-HBc became undectable and ALT levels normalized, either spontaneously or after interferon therapy in 12 (36.3%) of 33 patients with an exclusive wild-type viraemia, but only in two (5.7%) of 35 patients with homogeneous HBeAg minus HBV ( p = 0.005). An HBeAg minus viraemia higher than 20% was associated, in both HBeAg- and anti-HBe-positive patients, with HBV-induced liver disease and an unfavourable outcome of hepatitis. These findings suggest that surgence of HBeAg defective HBV is a virus strategy to survive under peculiar conditions dictated by the interplay between HBV and the host's immune system. The HBeAg/anti-HBe serological status is determined not only by the extent of virus replication and integration of HBV-DNA into cellular DNA but also by heterogeneity of HBV. The study of HBV heterogeneity in baseline sera of patients undergoing antiviral therapy appears to have a predictive value of the outcome of HBV infection in the single patient.
Published: 1991
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7. CMS physics technical design report, volume II: Physics performance

Author: G. Russo, R. Halsall, H. L. Zhuang, Stephan Linn, Stefanos Dris, J.F. Gunion, D. Delikaris, David Mark Raymond, Christophe Delaere, J. Caballero Bejar, Alex Kamenev, Victor Kim, Armando Lanaro, Sajjad Asghar, Vivek Sharma, P. Mereu, S. B. Kim, M. G. Albrow, Kostas Kloukinas, R. A. Kvatadze, Y. Velikzhanin, C. Mavrommatis, J. T. Rhee, M. Guerzoni, Robert M Harris, F. Romano, Daniele Spiga, Flera Rizatdinova, Dmitry Gorbunov, Giacomo Bruno, Laszlo Boldizsar, W. E. Johns, B. De Callatay, Pushpalatha C Bhat, M. Pioppi, Lawrence Sulak, L. J. Gutay, M. Hashemi, W. Badgett, A. Bartoloni, C. Y. Chien, E. Marinova, Daniel Robert Marlow, J. M. Barcala, C. Sabev, Roberto Sacchi, P. Bartalini, B. Panev, J. Reinhold, Yves Sirois, J. Ying, M. Demarteau, Alicia Calderon, Arno Heister, Lothar At Bauerdick, Marco Meschini, R. Wilkinson, Tejinder Virdee, Mirko Planinic, G. Martinelli, Y. Ershov, Piotr Traczyk, M. Ageron, Kirill Prokofiev, S. De Visscher, W. Bertl, Julia Thom, D. S. Bailey, N. Manna, Francesco Navarria, A. Karar, Pierre Ramond, Lucia Silvestris, R. Eichler, Neeti Parashar, S. Cucciarelli, R. Gokieli, Christopher George Tully, L. Masetti, I. Gonzalez Cabellero, Klaus Rabbertz, S. Zelepoukine, Sebastiano Albergo, A. Bonnevaux, P. Fabbricatore, Claudio Campagnari, V. Timciuc, O. Zorba, Igor Lokhtin, Ezio Maina, Colin Bernet, Nadia Pastrone, M. Galanti, K. Marasovic, Jim Kowalkowski, M. Zielinski, P. Aspell, Teppo Mäenpää, Pamela Klabbers, V. B. Francis, I. Diaz Merino, H. A. Sanchez Hernandez, Valery Zhukov, Christos Leonidopoulos, Gyorgy Bencze, Dimitri Bourilkov, Vassili Kachanov, Marina Passaseo, Patrick Slattery, J. Schümann, K. Cho, Hans-Christian Kaestli, Filip Moortgat, Francesca Nessi-Tedaldi, S. K. Lalwani, P. Trüb, D. Wenman, Aliaksandr Litomin, P. Siegrist, S. K. Oh, M. Kossov, A. Slav, Nicanor Colino, Z. Wlodarczyk, J. Amundson, H. Mathez, A. Papageorgiou, Pete Markowitz, J. Blocki, I. Soric, L. Stepanova, Pierluigi Paolucci, Amitabh Lath, Jianhong Wu, N. Sinanis, R. Imlay, Dario Menasce, T. Grassi, S. Lusin, H. von Gunten, T. Weiler, Gyorgy Vesztergombi, J. P. Dewulf, G. Marian, B. A. Barnett, N. Iyurin, Frank Jm Geurts, T. Hesselroth, Nikolaos Manthos, Martin Grunewald, Oleg Prokofyev, Wolfram Erdmann, V. Elsha, A. Krokhotin, Livio Fanò, David Barney, Stephanie Beauceron, Mark Raymond Adams, Nicolo Cartiglia, Alexander Oh, Safdar Abbas, Giorgio Apollinari, M. Jenkins, G. Belli, E. E. Boos, Elena Accomando, M. Afaq, Yan-Lin Ye, M. Serin-Zeyrek, Karl Gill, H. S. Chen, Roland Horisberger, S. Mersi, Serguei Ganjour, P. Tempesta, Sergio Cittolin, Julien Caudron, I. Schmidt, Stefan Schael, R. Dabrowski, Sudhir Malik, M. Cardenas Montes, P. S. Flower, K. Gabathuler, T. L. Watts, D. A. Sanders, V. Bhandari, G. Lukhanin, Y. Fu, S. Vassiliev, I. Legrand, Srecko Morovic, Francois Vasey, Yuan Chao, Y. 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Filine, Pascal Paganini, A. Roy, N. Zamiatin, S. Paktinat Mehdiabadi, Vladimir Andreev, Aruna Nayak, I. Jimenez, Archana Sharma, Saptaparna Bhattacharya, Laird Kramer, Andrey Korytov, P. Chang, Eduardo De Moraes Gregores, John H. Wickens, Vladimir Korenkov, N. Magini, A. Moggi, I. Crotty, Jan Krolikowski, Duccio Abbaneo, V. Litvine, Elizabeth Locci, M. I. Asghar, A. Kapusi, Y. Zhang, C. Bloch, V. Sknar, Vadim Issakov, Giovanni Polese, Alexander Toropin, J. Nysten, E. Forton, Y. Liu, S. Wang, M. Jaworski, H. Foeth, Belforte, S., Cossutti, F., DELLA RICCA, Giuseppe, Gobbo, B., Penzo, A., Laboratoire d'Annecy de Physique des Particules (LAPP/Laboratoire d'Annecy-le-Vieux de Physique des Particules), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS), Département d'Astrophysique, de physique des Particules, de physique Nucléaire et de l'Instrumentation Associée (DAPNIA), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Laboratoire Leprince-Ringuet (LLR), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS), Département Recherches Subatomiques (DRS-IPHC), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS), Institut de Physique Nucléaire de Lyon (IPNL), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Institut Pluridisciplinaire Hubert Curien (IPHC), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), informatique, Université de Lyon-Université de Lyon-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), CMS, Institut de Physique des 2 Infinis de Lyon (IP2I Lyon), Centre National de la Recherche Scientifique (CNRS)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Laboratoire d'Annecy de Physique des Particules (LAPP), Centre National de la Recherche Scientifique (CNRS)-École polytechnique (X)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3), Université de Strasbourg (UNISTRA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Bayatian, G, Chatrchyan, S, Hmayakyan, G, Sirunyan, A, Adam, W, Bergauer, T, Dragicevic, M, Ero, J, Friedl, M, Fruehwirth, R, Ghete, V, Glaser, P, Hrubec, J, Jeitler, M, Krammer, M, Magrans, I, Mikulec, I, Mitaroff, W, Noebauer, T, Pernicka, M, Porth, P, Rohringer, H, Strauss, J, Taurok, A, Waltenberger, W, Walzel, G, Widl, E, Wulz, C, Fedorov, A, Korzhik, M, Missevitch, O, Zuyeuski, R, Chekhovsky, V, Dvornikov, O, Emeliantchik, I, Litomin, A, Mossolov, V, Shumeiko, N, Solin, A, Stefanovitch, R, Gonzalez, J, Tikhonov, A, Petrov, V, D'Hondt, J, De Weirdt, S, 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S, Hagopian, V, Jenkins, M, Johnson, K, Prosper, H, Wahl, H, Baarmand, M, Baksay, L, Guragain, S, Hohlmann, M, Mermerkaya, H, Ralich, R, Vodopiyanov, I, Adams, M, Betts, R, Gerber, C, Shabalina, E, Smith, C, Ten, T, Akgun, U, Ayan, A, Cooper, A, Debbins, P, Duru, F, Fountain, M, George, N, Mccliment, E, Merlo, J, Mestvirishvili, A, Miller, M, Newsom, C, Norbeck, E, Onel, Y, Schmidt, I, Wang, S, Anderson, E, Atramentov, O, Hauptman, J, Lamsa, J, Barnett, B, Blumenfeld, B, Chien, C, Maksimovic, P, Spangler, S, Swartz, M, Baringer, P, Bean, A, Coppage, D, Grachov, O, Kim, E, Murray, M, Bandurin, D, Bolton, T, Khanov, A, Maravin, Y, Onoprienko, D, Rizatdinova, F, Sidwell, R, Stanton, N, Von Toerne, E, Baden, D, Bard, R, Eno, S, Grassi, T, Hadley, N, Kellogg, R, Kunori, S, Ratnikov, F, Skuja, A, Arcidiacono, R, Ballintijn, M, Bauer, G, Harris, P, Kravchenko, I, Loizides, C, Nahn, S, Paus, C, Pavlon, S, Roland, C, Roland, G, Sumorok, K, Vaurynovich, S, Veres, G, Wyslouch, B, Bailleux, D, Corum, S, Cushman, P, De Benedetti, A, Dolgopolov, A, Egeland, R, Franzoni, G, Gilbert, W, Grahl, J, Haupt, J, Kubota, Y, Mans, J, Pearson, N, Rusack, R, Singovsky, A, Cremaldi, L, Godang, R, Kroeger, R, Sanders, D, Summers, D, Bloom, K, Claes, D, Dominguez, A, Eads, M, Lundstedt, C, Malik, S, Snow, G, Sobol, A, Iashvili, I, Kharchilava, A, Alverson, G, Barberis, E, Boeriu, O, Eulisse, G, Musienko, Y, Muzaffar, S, Osborne, I, Reucroft, S, Swain, J, Taylor, L, Tuura, L, Wood, D, Gobbi, B, Kubantsev, M, Schellman, H, Spencer, E, Velasco, M, Baumbaugh, B, Cason, N, Hildreth, M, Karmgard, D, Marinelli, N, Ruchti, R, Warchol, J, Wayne, M, Bylsma, B, Durkin, L, Gilmore, J, Gu, J, Herman, D, Killewald, P, Knobbe, K, Ling, T, Elmer, P, Marlow, D, Piroue, P, Stickland, D, Tully, C, Wildish, T, Wynhoff, S, Xie, Z, Apresyan, A, Arndt, K, Banicz, K, Barnes, V, Bolla, G, Bortoletto, D, Bujak, A, Garfinkel, A, Lopez, O, Gutay, L, Ippolito, N, Kozhevnikov, Y, Laasanen, A, Liu, C, Maroussov, V, Merkel, P, Miller, D, Miyamoto, J, Neumeister, N, Rott, C, Roy, A, Sedov, A, Shipsey, I, Parashar, N, Eppley, G, Lee, S, Liu, J, Matveev, M, Nussbaum, T, Padley, B, Roberts, J, Tumanov, A, Yepes, P, Bodek, A, Budd, H, Chung, Y, De Barbaro, P, Demina, R, Eusebi, R, Ginther, G, Gotra, Y, Hocker, A, Husemann, U, Korjenevski, S, Sakumoto, W, Slattery, P, Tipton, P, Zielinski, M, Bartz, E, Doroshenko, J, Halkiadakis, E, Jacques, P, Kalelkar, M, Khits, D, Lath, A, Macpherson, A, Perera, L, Plano, R, Rose, K, Schnetzer, S, Somalwar, S, Stone, R, Thomson, G, Watts, T, Akchurin, N, Carrell, K, Gumus, K, Jeong, C, Kim, H, Papadimitriou, V, Sill, A, Spezziga, M, Washington, E, Wigmans, R, Bapty, T, Engh, D, Johns, W, Keskinpala, T, Lopez, E, Neema, S, Nordstrom, S, Pathak, S, Sheldon, P, Vaandering, E, Webster, M, Arenton, M, Conetti, S, Cox, B, Hirosky, R, Imlay, R, Ledovskoy, A, Phillips, D, Powell, H, Ronquest, M, Smith, D, Baek, Y, Bellinger, J, Bradley, D, Carlsmith, D, Crotty, I, Dasu, S, Feyzi, F, Gorski, T, Grothe, M, Hogg, W, Jaworski, M, Klabbers, P, Lanaro, A, Loveless, R, De Abril, M, Reeder, D, Smith, W, Wenman, D, Atoyan, G, Dhawan, S, Issakov, V, Neal, H, Poblaguev, A, Zeller, M, Yuldashev, B, TR1918, Demir, Durmuş Ali, Izmir Institute of Technology. Physics, Çukurova Üniversitesi, Fen-Edebiyat Fakültesi, Fizik Bölümü, and Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Physics beyond the Standard Model, Large Hadron Collider (LHC), 01 natural sciences, 7. Clean energy, HIGGS-BOSON PRODUCTION, TO-LEADING-ORDER, PRODUCTION CROSS-SECTION, LARGE HADRON COLLIDER, SUPERSYMMETRIC STANDARD MODEL, ELECTROWEAK SYMMETRY-BREAKING, LARGE TRANSVERSE-MOMENTUM, B-C MESON, SUPERCONDUCTING SUPER COLLIDER, SOFTLY BROKEN SUPERSYMMETRY, Vector boson, Settore FIS/04 - Fisica Nucleare e Subnucleare, PARTICLE PHYSICS, CMS, Energy density, technical design report, CERN, [PHYS.HEXP]Physics [physics]/High Energy Physics - Experiment [hep-ex], Physics, Large Hadron Collider, Settore FIS/01 - Fisica Sperimentale, Supersymmetry, Physics and Astronomy (all), Nuclear and High Energy Physics, FIS/01 - FISICA SPERIMENTALE, High-density QCD matter, Higgs boson, CMS, LHC, Physics, particles, Higgs, particle physics, detector, accelerator, Particle physics, Standard Model, Settore FIS/03 - Fisica della Materia, Nuclear physics, 0103 physical sciences, 010306 general physics, Settore MAT/07 - Fisica Matematica, 010308 nuclear & particles physics, High Energy Physics::Phenomenology, Little Higgs, pp, LHC, Higgs, supersymmetry, heavy ion, High Energy Physics::Experiment, CERN Large Hadron Collider (LHC), Parton momentum fraction, Minimal Supersymmetric Standard Model
Abstract: CMS is a general purpose experiment, designed to study the physics of pp collisions at 14 TeV at the Large Hadron Collider (LHC). It currently involves more than 2000 physicists from more than 150 institutes and 37 countries. The LHC will provide extraordinary opportunities for particle physics based on its unprecedented collision energy and luminosity when it begins operation in 2007. The principal aim of this report is to present the strategy of CMS to explore the rich physics programme offered by the LHC. This volume demonstrates the physics capability of the CMS experiment. The prime goals of CMS are to explore physics at the TeV scale and to study the mechanism of electroweak symmetry breaking - through the discovery of the Higgs particle or otherwise. To carry out this task, CMS must be prepared to search for new particles, such as the Higgs boson or supersymmetric partners of the Standard Model particles, from the start-up of the LHC since new physics at the TeV scale may manifest itself with modest data samples of the order of a few fb-1 or less. The analysis tools that have been developed are applied to study in great detail and with all the methodology of performing an analysis on CMS data specific benchmark processes upon which to gauge the performance of CMS. These processes cover several Higgs boson decay channels, the production and decay of new particles such as Z′ and supersymmetric particles, Bs production and processes in heavy ion collisions. The simulation of these benchmark processes includes subtle effects such as possible detector miscalibration and misalignment. Besides these benchmark processes, the physics reach of CMS is studied for a large number of signatures arising in the Standard Model and also in theories beyond the Standard Model for integrated luminosities ranging from 1 fb-1 to 30 fb-1. The Standard Model processes include QCD, B-physics, diffraction, detailed studies of the top quark properties, and electroweak physics topics such as the W and Z0 boson properties. The production and decay of the Higgs particle is studied for many observable decays, and the precision with which the Higgs boson properties can be derived is determined. About ten different supersymmetry benchmark points are analysed using full simulation. The CMS discovery reach is evaluated in the SUSY parameter space covering a large variety of decay signatures. Furthermore, the discovery reach for a plethora of alternative models for new physics is explored, notably extra dimensions, new vector boson high mass states, little Higgs models, technicolour and others. Methods to discriminate between models have been investigated. This report is organized as follows. Chapter 1, the Introduction, describes the context of this document. Chapters 2-6 describe examples of full analyses, with photons, electrons, muons, jets, missing E T, B-mesons and τ's, and for quarkonia in heavy ion collisions. Chapters 7-15 describe the physics reach for Standard Model processes, Higgs discovery and searches for new physics beyond the Standard Model. © 2007 IOP Publishing Ltd.
Published: 2007
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8. Wild-type and e antigen-minus hepatitis B viruses and course of chronic hepatitis

Author: Hans Will, M. Giarin, Maurizia Rossana Brunetto, A Alfarano, A Serra, Giorgio Verme, Giorgio Maria Saracco, Filippo Oliveri, Maurizio Baldi, and E. Chiaberge
Subjects: Adult, Male, Hepatitis B virus, Adolescent, viruses, Molecular Sequence Data, Biology, medicine.disease_cause, Polymerase Chain Reaction, Virus, Liver disease, medicine, Immune Tolerance, Humans, Hepatitis B e Antigens, Hepatitis B Antibodies, Aged, Hepatitis, Multidisciplinary, Base Sequence, Liver cell, virus diseases, Nucleic Acid Hybridization, Hepatitis B, Middle Aged, medicine.disease, biology.organism_classification, Virology, digestive system diseases, HBeAg, Hepadnaviridae, Immunology, DNA, Viral, Mutation, Female, Research Article
Abstract: Using an oligonucleotide hybridization assay, we studied the clinical implication of wild-type hepatitis B virus (HBV) and a HBV mutant that is unable to secrete hepatitis B e antigen (HBeAg) because of a translational defect due to a stop codon in the pre-C region in 106 hepatitis B surface antigen-positive patients with chronic hepatitis B. Wild-type HBV was detected in 31 of 42 (73.8%) HBeAg-positive patients, whereas a mixed viral population was present in 10 (23.8%). Significant differences in the severity and outcome of liver disease were not observed in the two groups of patients. However, the emergence of HBeAg-minus HBV in wild-type HBV carriers was associated with an exacerbation of liver disease and was followed by the presence of antibodies against HBeAg (anti-HBe) in serum in 50% of the cases. In 61 of 64 (95.3%) anti-HBe-positive patients, HBeAg-minus HBV was the predominant virus: HBeAg-minus HBV was detected in 42 patients (65.6%), whereas both wild-type and HBeAg-minus HBV were present in 19 (29.7%). HBeAg-minus HBV was associated with a course of hepatitis characterized by flare-ups of liver cell necrosis interspersed with periods of asymptomatic HBV carriage (P less than 0.01). These data support the hypothesis that genetic heterogeneity of HBV significantly influences the course and outcome of chronic hepatitis B. Wild-type HBV secreting HBeAg induces immunologic tolerance and causes chronic infection. HBeAg-minus HBV might be unable to induce chronic infection without the helper function of wild-type HBV, but it appears to be more pathogenic. Once chronic infection is established, HBeAg-minus HBV variants may prevail and displace wild-type virus.
Published: 1991

9. Double Autologous Transplant Versus Tandem Autologus - Non Myeloablative Allogeneic Transplant for Newly Diagnosed Multiple Myeloma

Author: Patrizia Falco, Paola Omedè, Mario Boccadoro, Maria Teresa Ambrosini, G. Ciccone, Bernardino Allione, Luisa Giaccone, Massimo Aglietta, Marcello Rotta, Andrea Gallamini, A. Levis, Nicola Mordini, Benedetto Bruno, Fabrizio Carnevale-Schianca, Rainer Storb, R Fanin, Daniele Mattei, M. Giarin, Antonio Palumbo, Sara Bringhen, Roberto Sorasio, Massimo Massaia, and Francesca Patriarca
Subjects: Melphalan, Immunofixation, medicine.medical_specialty, biology, Beta-2 microglobulin, business.industry, Immunology, Induction chemotherapy, Cell Biology, Hematology, medicine.disease, Biochemistry, Chemotherapy regimen, Gastroenterology, Surgery, Median follow-up, Internal medicine, Monoclonal, medicine, biology.protein, business, Multiple myeloma, medicine.drug
Abstract: Allogeneic approaches employing low dose TBI nonmyeloablative conditionings reported a dramatic reduction of transplant-related mortality (TRM) compared to conventional high dose regimens in newly diagnosed multiple myeloma (MM) (Maloney et al, Blood, 2003). The role of allografting, however, compared to autologous HCT remains to be determined. From September 1999 to July 2004, 241 consecutive MM patients, up to the age of 65, were diagnosed at five academic Italian Institutions. Overall, 194/241 had natural siblings (Table 1): 158/194 (81%) were HLA typed, while 36/194 (19%) were not typed for the following reasons: patients not eligible for high dose chemotherapy (n. 14); siblings not eligible for peripheral hematopoietic cell (PHC) donation (n.11); patient refusal to high dose chemotherapy (n. 9), unknown (n.2). Seventy-six/158 (48%) with a matched donor were offered a tandem autologous- nonmyeloablative allogeneic HCT approach. Eventually, 56/76 (73%), the “auto-allo group”, were enrolled while 20 did not enter the tandem program as 5 siblings (5/76, 7%) were not eligible for PHC donation, 5 patients refused an allogeneic HCT, and 10 patients preferred allografting as a possible salvage treatment. Of 102 patients without matched donors or after refusal to allografting, 73, “double-auto group”, underwent a standard double autologous transplant while 29 received less intense treatments because of clinical conditions or patient preference. Table 1 Newly diagnosed pts 241 With sibs/without sibs 194 /47 (total 241) HLA typed /not HLA typed 158 /36 (total 194) Matched sibs /No matched sibs 76 /82 (total 158) Auto-Allo”/“Double Auto”/Other”“ 56 /73 /29 (total 158) After induction chemotherapy, patients of both groups underwent G-CSF mobilised autografting with high dose melphalan (200 mg/m2). In the “auto-allo” group, the autologous HCT was followed, 2-4 months later, by low dose (2.0 Gy) TBI, allogeneic PHC infusion, and post transplant mycophenolate mofetil and cyclosporin. In the “double-auto group”, patients received a second autologous HCT. Patients characteristics were as follows: age: 54 (range 34–65) vs 53 (range 33–64) (p=ns); stage III myeloma: 77% vs 64% (p=0.03); beta 2 microglobulin > 2,5 mg/dl: 75% vs 59% (p=0.005), for the “auto-allo group” and for “the double-auto group”, respectively. At the time of this analysis, 56/56 of the “auto-allo group” and 55/73 of “the double-auto group” had completed the transplant programs. After median follow up of 3 years (range 11–80 months), TRM was 11% vs 4% (p=0.09); complete remission rates, defined as the disappearance of the monoclonal paraprotein by immunofixation, were 46% vs 16% (p=0.0001); overall survivals were 84% versus 62% (p=0.003); progression free survivals were 75% vs 41% (p=0.00008); event free survivals were 61% (34/56)% vs 38% (30/73) (p=0.006) in the “auto-allo group” and in the “double-auto” group, respectively. Longer follow up is needed, however data suggest that the “auto- non myeloablative allo” approach is not inferior to “double autologous” HCT in newly diagnosed MM.
Published: 2005
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10. Wild-type and 'o' antigen minus hepatitis B viruses and course of chronic hepatitis B in children

Author: Maurizia Rossana Brunetto, M. Giarin, Cristiana Barbera, Ferruccio Bonino, T. Parrella, and M.L. Abate
Subjects: Hepatology, Antigen, Chronic hepatitis, business.industry, Wild type, Medicine, Hepatitis B, business, medicine.disease, Virology
Published: 1991
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11. Wild type and HBeAG minus HBVs in peripheral blood lymphocytes (PBL) from fulmonant hepatitis (FH)

Author: Maurizia Rossana Brunetto, Giovanni C. Actis, M. Giarin, Alfredo Marzano, U. Dianzani, and W. Redoglia
Subjects: Hepatitis, Hepatology, HBeAg, business.industry, Immunology, Wild type, Medicine, business, medicine.disease, Virology, Peripheral blood
Published: 1991
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12. HBV heterogeneity and response to interferon

Author: Filippo Oliveri, Pier Luigi Calvo, Paola Manzini, Ferruccio Bonino, M. Giarin, Maurizia Rossana Brunetto, Giorgio Verme, Giorgio Maria Saracco, and A. Demartini
Subjects: Hepatology, Interferon, medicine, Biology, Virology, medicine.drug
Published: 1991
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13. Chasing the Zebra: a case of membranous-like Glomerulopathy with SSA/RO52 deposits and no overt connective tissue disease.

Author: Sciascia S, Miraglia P, Radin M, Giarin M, Charbonier N, Barreca A, Cecchi I, Lanzetta I, Fenoglio R, Menegatti E, and Roccatello D
Abstract: Background: The nature of the deposits in immune-mediated glomerulonephritis with a membranous pattern and masked IgG-Kappa deposits (MGMID) remains still to be elucidated., Case Presentation: We present a case of 33-year-old woman developing a continuous asymptomatic proteinuria (0.8-1 g/24 h) with no overt connective tissue diseases. She tested positive at high titers for SSA antibodies (Ro52 838 UI/mL, Ro60 2716 UI/mL) and at the kidney biopsy histological findings were compatible with an immune-mediated glomerulonephritis with a membranous pattern and masked IgG-Kappa deposits. Also, we demonstrated a positive immunohistochemistry staining for anti-Ro52-SSA antibodies, with a granular positivity in mesangium and along rare glomerular capillaries. To date, only one case of a patient with overt diagnosis of Sjögren's syndrome with MGMID has been described but a pathogenic role for SSA and SSB antibodies has never been proven., Conclusions: In this case, we described for the first time by immunohistochemistry a Ro52+ granular positivity in the mesangium and glomerular capillaries, potentially paving the way for a better understanding of MGMID., (© 2023. The Author(s).)
Published: 2023
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14. Immunohistochemical typing of amyloid in fixed paraffin-embedded samples by an automatic procedure: Comparison with immunofluorescence data on fresh-frozen tissue.

Author: Barreca A, Bottasso E, Veneziano F, Giarin M, Nocifora A, Martinetti N, Attanasio A, Biancone L, Benevolo G, Roccatello D, Cassoni P, and Papotti MG
Subjects: Adult, Aged, Aged, 80 and over, Amyloid genetics, Amyloidogenic Proteins genetics, Amyloidosis classification, Amyloidosis genetics, Amyloidosis pathology, Female, Fluorescent Antibody Technique, Humans, Male, Mass Spectrometry, Middle Aged, Paraffin Embedding, Amyloid isolation & purification, Amyloidogenic Proteins isolation & purification, Amyloidosis diagnosis, Proteomics
Abstract: Amyloidosis comprises a spectrum of disorders characterized by the extracellular deposition of amorphous material, originating from an abnormal serum protein. The typing of amyloid into its many variants represents a pivotal step for a correct patient management. Several methods are currently used, including mass spectrometry, immunofluorescence, immunohistochemistry, and immunogold labeling. The aim of the present study was to investigate the accuracy and reliability of immunohistochemistry by means of a recently developed amyloid antibody panel applicable on fixed paraffin-embedded tissues in an automated platform. Patients with clinically and pathologically proven amyloidosis were divided into two cohorts: a pilot one, which included selected amyloidosis cases from 2009 to 2018, and a retrospective one (comprising all consecutive amyloidosis cases analyzed between November 2018 and May 2020). The above-referred panel of antibodies for amyloid classification was tested in all cases using an automated immunohistochemistry platform. When fresh-frozen material was available, immunofluorescence was also performed. Among 130 patients, a total of 143 samples from different organs was investigated. They corresponded to 51 patients from the pilot cohort and 79 ones from the retrospective cohort. In 82 cases (63%), fresh-frozen tissue was tested by immunofluorescence, serving to define amyloid subtype only in 30 of them (36.6%). On the contrary, the automated immunohistochemistry procedure using the above-referred new antibodies allowed to establish the amyloid type in all 130 cases (100%). These included: ALλ (n = 60, 46.2%), ATTR (n = 29, 22.3%), AA (n = 19, 14.6%), ALκ (n = 18, 13.8%), ALys (n = 2, 1.5%), and Aβ2M amyloidosis (n = 2, 1.5%). The present immunohistochemistry antibody panel represents a sensitive, reliable, fast, and low-cost method for amyloid typing. Since immunohistochemistry is available in most pathology laboratories, it may become the new gold standard for amyloidosis classification, either used alone or combined with mass spectrometry in selected cases., Competing Interests: The authors have declared that no competing interests exist.
Published: 2021
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15. Unitary permeability of gap junction channels to second messengers measured by FRET microscopy.

Author: Hernandez VH, Bortolozzi M, Pertegato V, Beltramello M, Giarin M, Zaccolo M, Pantano S, and Mammano F
Subjects: Connexin 26, Connexins biosynthesis, Cyclic AMP physiology, Electrophysiology, HeLa Cells, Humans, Inositol Phosphates physiology, Ion Channel Gating physiology, Microscopy, Fluorescence, Permeability, Transfection, Biosensing Techniques methods, Fluorescence Resonance Energy Transfer, Gap Junctions metabolism, Ion Channels metabolism, Second Messenger Systems physiology
Abstract: Gap junction channels assembled from connexin protein subunits mediate intercellular transfer of ions and metabolites. Impaired channel function is implicated in several hereditary human diseases. In particular, defective permeation of cAMP or inositol-1,4,5-trisphosphate (InsP(3)) through connexin channels is associated with peripheral neuropathies and deafness, respectively. Here we present a method to estimate the permeability of single gap junction channels to second messengers. Using HeLa cells that overexpressed wild-type human connexin 26 (HCx26wt) as a model system, we combined measurements of junctional conductance and fluorescence resonance energy transfer (FRET) emission ratio of biosensors selective for cAMP and InsP(3). The unitary permeabilities to cAMP (47 x 10(-3) +/- 15 x 10(-3) microm(3)/s) and InsP(3) (60 x 10(-3) +/- 12 x 10(-3) microm(3)/s) were similar, but substantially larger than the unitary permeability to lucifer yellow (LY; 7 +/- 3 x 10(-3) microm(3)/s), an exogenous tracer. This method permits quantification of defects of metabolic coupling and can be used to investigate interdependence of intercellular diffusion and cross-talk between diverse signaling pathways.
Published: 2007
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16. Hepatitis B virus unable to secrete e antigen and response to interferon in chronic hepatitis B.

Author: Brunetto MR, Giarin M, Saracco G, Oliveri F, Calvo P, Capra G, Randone A, Abate ML, Manzini P, and Capalbo M
Subjects: Adolescent, Adult, Aged, Analysis of Variance, Base Sequence, DNA, Viral analysis, DNA, Viral genetics, Female, Hepatitis B drug therapy, Hepatitis B pathology, Hepatitis B e Antigens analysis, Hepatitis B virus genetics, Hepatitis B virus isolation & purification, Humans, Immunohistochemistry, Interferons therapeutic use, Liver metabolism, Liver pathology, Male, Middle Aged, Molecular Sequence Data, Mutation genetics, Nucleic Acid Hybridization, Oligonucleotides, Viral Core Proteins genetics, Hepatitis B immunology, Hepatitis B e Antigens metabolism, Hepatitis B virus metabolism, Interferons pharmacology
Abstract: Background: Anti-hepatitis e antigen-positive chronic hepatitis B is a progressive liver disease associated with precore mutant hepatitis B virus (HBV) and poor response to interferon. Therefore, precore mutant HBV may behave as an interferon-resistant virus. The relations between the prevalences of wild-type and precore mutant HBVs in baseline viremias and response to interferon were analyzed., Methods: Sera from 115 patients (59 treated and 56 untreated, followed up for 30 months) were tested using a quantitative oligonucleotide hybridization assay., Results: Spontaneous or interferon-induced recoveries were observed in 28.5% (6 of 21) and 47.3% (18 of 38) or in 0% (0 of 35) and 19% (4 of 21) of the patients with wild-type prevalent or mutant prevalent HBVs, respectively. Relapses occurred in 85.7% (12 of 14) and 19.4% (4 of 21) of treated patients with prevalent precore mutant and prevalent wild-type HBV, respectively (P = 0.0001). High precore mutant HBV levels (> 20% of total viremia) were associated with the lack of permanent response to interferon (P = 0.01)., Conclusions: Precore mutant HBV can influence the response to interferon when it reaches significant serum levels (> 20% of total viremia). Therefore, chronic hepatitis B should be treated as early as possible in its natural history before precore mutant HBV is selected as a prevalent virus.
Published: 1993
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17. Different aspects of HDV and HBV reinfections and liver disease in orthotopic liver transplant (OLT).

Author: Smedile A, Mangia A, Brunetto MR, Giarin M, Marzano A, Ottobrelli A, Chiaberge E, Durazzo M, Bonino F, and Verme G
Subjects: Adult, Female, Hepatitis B complications, Hepatitis B microbiology, Hepatitis B virus genetics, Hepatitis B virus isolation & purification, Hepatitis D complications, Hepatitis, Chronic complications, Hepatitis, Chronic surgery, Humans, Liver Cirrhosis etiology, Male, Middle Aged, Mutation, Recurrence, Hepatitis B surgery, Hepatitis D surgery, Liver Transplantation
Published: 1993

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1. ICARUS at the Fermilab Short-Baseline Neutrino program: initial operation

2. Overhaul and Installation of the ICARUS-T600 Liquid Argon TPC Electronics for the FNAL Short Baseline Neutrino Program

3. CMS technical design report, volume II: Physics performance

4. Quantitative analysis of wild-type and HBeAg minus hepatitis B viruses by a sequence-dependent primer extension assay

5. Serum free light chain ratio, total kappa/lambda ratio, and immunofixation results are not prognostic factors after stem cell transplantation for newly diagnosed multiple myeloma

6. ‘e’ Antigen defective hepatitis B virus and course of chronic infection

7. CMS physics technical design report, volume II: Physics performance

8. Wild-type and e antigen-minus hepatitis B viruses and course of chronic hepatitis

9. Double Autologous Transplant Versus Tandem Autologus - Non Myeloablative Allogeneic Transplant for Newly Diagnosed Multiple Myeloma

10. Wild-type and 'o' antigen minus hepatitis B viruses and course of chronic hepatitis B in children

11. Wild type and HBeAG minus HBVs in peripheral blood lymphocytes (PBL) from fulmonant hepatitis (FH)

12. HBV heterogeneity and response to interferon

13. Chasing the Zebra: a case of membranous-like Glomerulopathy with SSA/RO52 deposits and no overt connective tissue disease.

14. Immunohistochemical typing of amyloid in fixed paraffin-embedded samples by an automatic procedure: Comparison with immunofluorescence data on fresh-frozen tissue.

15. Unitary permeability of gap junction channels to second messengers measured by FRET microscopy.

16. Hepatitis B virus unable to secrete e antigen and response to interferon in chronic hepatitis B.

17. Different aspects of HDV and HBV reinfections and liver disease in orthotopic liver transplant (OLT).

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