Search

Objective: To prove the effect of the combination of cinnamon bark extract with cisplatin in reducing efflux and increasing influx in SKOV3 ovarian cancer cell cultures by measuring the expression of p-glycoprotein, CTR1 and the annexim V. Methods: This research is an experimental study using SKOV3 ovarian cancer cells stored in the SCTE IMERI FKUI Laboratory, carried out in the Stem Cells and Tissues Engineering Research Cluster laboratory. The cells were then harvested by adding trypsin-EDTA to the culture as much as 1 mL, and rotated at 2000 rpm for 5 minutes. Then the cells were added with antibodies and dissolved with a stain buffer solution and read on a flow cytometry device. We used ethyl acetate extract from cinnamon bark against the SKOV3 cell line . IC50 of Cinnamon bark extract we got from MTS test. We tested the levels of IC50, 3/4 IC50, 1/2 IC50, and 1/4 IC50 of cinnamon bark extract with a combination of IC50, 3/4 IC50 , 1/2 IC50, and 1/4 IC50 cisplatin against the viability of the SKOV3 cell line with single cisplatin IC50 comparator. We also examined the levels of annexin V as a marker of apoptosis in the SKOV3 cell line to see if the cell cycle arrest induced by cinnamon bark extract could cause apoptosis of the SKOV3 cell line. We assessed the sample distribution using the Shapiro-Wilk test because of the sample size . To assess the comparison of parameters (differences in p Glycoprotein and CTR-1 expression between treatment groups in normally distributed data, the test was used analysis of variance (ANOVA). ANOVA is a comparative test to analyze the difference in the mean (mean) of data from two or more variables in the same population. The Bonferroni test was used to analyze the same or different samples (equal and unequal) in each treatment. Results: From this study, it was found that the combination of IC 50 cinnamon bark extract and IC 50 cisplatin was able to lower p-glycoprotein levels higher with a lower mean value than the other treatment groups with p 0.001. In the test group, the highest CTR1 expression was found in the combination 1 test group, namely the 1 x IC50 combination. The value of CTR1 expression in the combination group 1 was 12%. Conclusion: The combination of cinnamon bark extract with cisplatin was shown to reduce efflux by decreasing p-glycoprotein expression and increasing influx by increasing CTR1 expression in SKOV3 ovarian cancer cell cultures.

Background: Ovarian cancer is fifth most common female cancer and third most common cancer in Indonesia, but most are advanced stage patients that experiencing recurrence, which indicates resistance to treatment especially to cisplatin. Melatonin appears as an alternative that can support apoptotic effect of cisplatin as a chemotherapy regimen. Aim: To determine effect of combination melatonin and cisplatin compared with cisplatin only chemotherapy on chemotherapy resistance with Copper Transporting ATPase-1 (CTR-1), P-glycoprotein (P-Gp), and Gamma-Glutamylcysteinylglycine (GSH) biomarkers in ovarian cancer biological cells SKOV3 Methods: This research design was experimental laboratory, post-test only control group design, using SKOV3 cell culture. This study was performed in the SCTE IMERI FKUI laboratory and Integrated Laboratory FKUI. MTS assay was used to calculate IC50 of each materials. The materials used were melatonin (concentration was 25,50,100,200,300 nM), cisplatin (concentration was 0.1, 0.5, 1, 2, 5 mM), and doxorubicin (concentration 10,20,40,50,80,100,200 µM). IC50 melatonin was 1,841 mM, IC50 cisplatin was 117,5 µM, and IC50 doxorubicin was 14,72 µM. Samples were control negative group, IC50 doxorubicin as a control positive, IC50 cisplatin, IC50 melatonin, combination group of melatonin and cisplatin were 1xIC50, ¾xIC50, ½xIC50, and ¼xIC50. ANOVA and Bonferroni test were used for statistical test. Results: Based on data processing, IC50 of melatonin was 1,841 mM, IC50 of doxorubicin was 14,72 mM, while IC50 of cisplatin was 117.5 μM. The mean expression of CTR-1 in IC50 melatonin group was 15.77 ± 0.21 and in IC50 cisplatin group was 10.87 ± 0.91, mean expression in IC50 doxorubicin group was 30,33 ± 0,4. Meanwhile, mean expression of CTR-1 in IC50 cisplatin was 7,37±0,7, and in combination 1 group (1xIC50 melatonin and 1xIC50 cisplatin) was 19,73±1.0,49. For P glycoprotein, mean expression in IC50 cisplatin was 16±1,59, in IC50 melatonin group was 7,37±0,21, in IC50 doxorubicin was 0, and in combination 1 group (1xIC50 melatonin and 1xIC50 cisplatin) was 6,7±0,17. Last, in GSH, mean expression in IC50 cisplatin group was 33,2±0,87, in IC50 melatonin group was 12,57±0,12, in IC50 doxorubicin group was 1,33±0,66, and in combination 1 group (1xIC50 melatonin and 1xIC50 cisplatin) was 11,73±0,67. There was significant difference of CTR-1 expression in combination 1 group which was higher (19.73%), p-glycoprotein expression in combination 1 group which was lower (6,7%), and also GSH expression in combination 1 group was lower (11,73%) compared to other groups. Conclusion: The group 1 combination of 1xIC50 melatonin and 1x IC50 cislatin with 1.841 mM and cisplatin 117.5 uM were able to reduce cisplatin chemotherapy resistance by increasing drug influx activity by increasing CTR-1 expression, decreasing drug efflux through decreasing p-glycoprotein expression, and decreased DNA repair activity through decreased GSH expression.

COVID-19 is a world health problem with the first cases found in Wuhan, China. This case progressed until there were reports of deaths and there was a spread outside China, including Indonesia. The Government of the Republic of Indonesia responded to this as an emergency response by establishing a task force to accelerate the handling of corona which functions to prevent, detect and respond to Covid -19. One form of prevention of transmission is to increase knowledge by conducting counseling. Method: Cross sectional study with 165 participants. This study uses a questionnaire measuring tool that is accessed online and analyzed into univariate and bivariate Result: There was a significant difference in people's knowledge before and after counseling with a value of p = 0.000. This is indicated by an increase in the post-test scores of participants who also experienced an increase after counseling with a mean post-test score of 74.2 compared to the pre-test score of 40.4. Conclusion: General Public knowledge and understanding of Covid-19 is still quite low so that counseling through the online system has succeeded in increasing public knowledge which can be seen from the post-test scores that have increased Keywords: COVID - 19, online, perception, knowledge.

BACKGROUND: Cisplatin (CIS) is an important chemotherapy agent which is widely used for the treatment of many types of solid tumors, which can cause decreased ovarian function. Nigella sativa has been shown to have an anti-inflammatory and anti-oxidant activity that might protect the ovaries from damage due to CIS. AIM: This study aims to understand the benefits of N. sativa protecting the ovaries due to CIS chemotherapy. METHODS: Thirty-two female Rattus norvegicus aged 8 weeks weighing 160–200 g were divided into four groups: Negative control, Positive control, Treatment-1 (CIS 6 mg/KgBW and NS 500 mg/KgBW/day), and Treatment-2 (CIS 6 mg/KgBW and NS 1000 mg/kgBW/day) for 2 weeks. On the 14th day the rats were sacrificed, blood was drawn from the heart, followed by taking ovaries. RESULTS: There was lower mortality and morbidity in CIS + NS 1000 and CIS + NS 500 mg group (p = 0.01 and 0.001). The mean estradiol levels, follicle-stimulating hormone levels, and anti-mullerian hormone levels were not statistically significant among the four groups. The highest number of primary, secondary, tertiary follicles are seen at the CIS + NS 500 mg and CIS + NS 1000 mg groups (p = 0.05). The lowest number of atretic follicles is seen at the CIS + NS 1000 mg group, and the highest number of atretic follicles was in CIS only. CONCLUSION: There is a trend that N. sativa is beneficial in protecting the ovaries from damage caused by CIS.

BACKGROUND: Candida albicans was the common causes of vulvovaginalis candidiasis (VVC) in human. To avoid complications, prompt and proper treatment of VVC must be performed. The pharmacological effects of Nigella sativa include antimicrobial, anti-inflammatory, immune stimulation, and anti-cancer properties. AIM: N. sativa has been shown to have an in vivo antifungal effect and the purpose of this study was to determine the antifungal and potential in vivo therapeutic effects. METHODS: This research was an empirical study which evaluated the therapeutic effect of the vaginal candidiasis model of N. sativa in rats. The subjects were 28 rats inoculated with C. albicans and were divided into four groups: Control group (G1), fluconazole group (G2), N. sativa group (G3), and N. sativa and fluconazole group combinations (G4). The colony of C. albicans was assessed to determine the treatment’s therapeutic effect. RESULTS: There was no difference in the number of colonies of C. albicans between all the pre-inoculation (p = 0.274) and post-inoculation (p = 0.323) classes. A substantial decrease in the number of C. albicans colonies within 48 h of treatment was observed between the three control group treatment forms (N. sativa group p = 0.046; fluconazole group p = 0.002; and N. sativa + fluconazole group p = 0.002). CONCLUSIONS: The therapeutic effect of N. sativa has been achieved by reducing the number of colonies of C. albicans.

Background: In postmenopausal women, decreasing estrogen levels is a marker of ovarian dysfunction. Hypoestrogenic state has known increasing the risk of osteoporosis. Objective: To determine the correlation between estradiol serum levels with classification of osteoporosis risk OSTA (Osteoporosis Self-Assessment Tools for Asian) in menopausal women. Methods: This study was case series study which examined estradiol serum in menopausal women by ELISA and assess the osteoporosis risk using osteoporosis risk classification OSTA. Total 47 samples was collected at Dr. H.Adam malik, dr. Pirngadi, and RSU Networking in Medan. This research was conducted from May to December 2016. Data were statistically analyzed, and presented with Spearman test. Results: In this study, we found the mean levels of estradiol in menopausal women was 18.62 ± 16.85 ng / ml with OSTA osteoporosis risk score of 2.09 ± 2.45. There was a significant positive correlation between estradiol and risk of osteoporosis OSTA with correlation coefficient r = 0.825 and p Conclusion: There is a strong positive correlation between serum levels of estradiol with OSTA osteoporosis risk assessment in menopausal women.

Reducing maternal mortality rate is a key concern of health promotion in developing countries or city face. The investigated and survey for maternal mortality had been done in Dairy City. There are 149 samples got from the survey directly in this area for 2017. In this study, we use a machine learning approach to train and test the data of maternal mortality. The aim of this study to classification maternal mortality in health promotion for reducing the maternal mortality rate in Dairi. The result of this study indicated the decision tree and Naïve Bayes are available to train and test the dataset. The accuracy of the decision tree of maternal mortality is 100 % and the Naïve Bayes model indicates 97.37 % of maternal mortality.

Introduction : Preeclampsia is a major cause in both maternal and perinatal mortality and morbidity. The etiopathogenesis of preeclampsia remains unclear but endothelial dysfunction plays important role. Stress oxidative in placenta produces free radicals such as superoxide anions (O2-) and H2O2, trophoblast debris, pro-inflammatory cytokines, and antiangiogenic factors which are thought to cause vascular endothelial dysfunction and causing excessive maternal inflammatory responses. Tumor Necrosis Factor-α (TNF-α) is considered as one of the potentially specific markers for preeclampsia. Aim : This study aimed to determine the effects of Phaleria macrocarpa (Scheff.) Boerl Extract on inflammation in endothelial cells by measuring the TNF-α level In preeclampsia-induced HUVEC. Method : HUVEC (Human Umbilical Vein Endothelial Cell) culture is an in vitro model widely used to study the pathogenesis of preeclampsia. Phaleria macrocarpa (Scheff.) Boerl also known as Mahkota Dewa is widely used as an anti-inflammation and antioxidant because of alkaloids, saponins, flavonoids and polyphenols properties. Result : Phaleria macrocarpa extract reduced TNF-α level siginifcantly at concentration of 3.906 μg/mL. Phaleria macrocarpa extract at concentration of 62.5 μg/mL reduced TNF-α level to normal level. Conclusion : Phaleria macrocarpa extract might be used as agent to overcome endothelial dysfunction in preeclampsia.