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Although children frequently engage in creative activities (in which they make foods and objects by hand), the development and scope of children’s thinking about handmade items is largely unexplored. In the present studies, we examined whether 4- to 12-year-old children at a local children’s museum (54% girls, 46% boys; 51% White, 11% Asian/Asian-American, 10% more than one group, 4% Latinx, 3% Black/African-American, 18% did not report race/ethnicity) would expect other people to prefer handmade over factory-made items, including foods and nonfoods. In Experiments 1 (n = 124) and 2 (n = 122), participants expected a child character to prefer items the character made themselves and items made by the character’s parent or a local person. However, this expectation did not persist at all costs: When considering imperfect handmade items in Experiment 3 (n = 122), children demonstrated a handmade preference when considering nonfoods made by a parent but demonstrated a factory-made preference when considering foods made by a parent. Children’s explanations were associated with their choices: When children’s explanations referred to emotions or relationships, they were more likely to select handmade items. When children referred to item features, they were more likely to select factory-made items. Across studies, we observed persistent age and gender effects: Children’s handmade preference increased with child age and girls demonstrated a more robust handmade preference than boys. These findings highlight children’s developing and nuanced reasoning about object value. At an early age, children consider who made an object as a contributor to its value.

Despite a rich knowledge base about infants' social learning and studies observing social referencing in other species in food contexts, we know surprisingly little about social learning about food among human infants. This gap in the literature is particularly surprising considering that feeding unfamiliar foods to infants is a very common experience as infants begin to eat solid foods. The present study examines whether parental social modeling influences infants' willingness to accept unfamiliar foods. In two Zoom sessions, parents will be asked to feed unfamiliar foods to their 6- to 24-month-old infants (different unfamiliar foods in each session). In both sessions, infants' food acceptance and rejection will be measured. In the first session, parents will be asked to do what they would typically do; spontaneous social modeling will be recorded. In the second session, parents will be instructed to model eating the unfamiliar food. We will examine associations between infants' willingness to eat unfamiliar foods, parent social modeling, and extant parent and infant characteristics.

The present studies examine developmental changes in the elicitors of disgust by examining adults' and children's ideas of what is disgusting. In three experiments, we asked adults and children between the ages of 3 and 12 to report what is "disgusting," what is "gross," or what might have caused someone to make a disgust face. In Study 1, parents of 3- to 12-year-old children (n = 120) were asked what they thought was disgusting and what they thought their children would find disgusting and completed a picky eating questionnaire to examine the extent to which children's eating habits may be related to disgust. In Studies 2 and 3 (n = 98 per study), children were asked what they thought was disgusting. In Study 3, children's parents also completed a questionnaire about their child's food pickiness. Typically eaten foods that were not contaminated or spoiled were frequently mentioned in all studies, both by children and their parents. There was considerable diversity in the disgust elicitors that were mentioned across participants, highlighting the importance of examining individual differences in the development of disgust. (PsycInfo Database Record (c) 2021 APA, all rights reserved).

This study investigated dance practice in psychosocial and functional aspects, and quality of life in children with Down syndrome and autism spectrum disorder. Children with DS and ASD, between 3 and 12 years old, attended a dance program during 16 sessions/lessons, lasting 60 min, twice a week, in suitable place. Functional Independence Measure (FIM), Childhood Autism Rating Scale SF-36 quality of life survey, and Knowledge, Attitude and Practice Inquiry (KAP Inquiry) were applied before and after dance classes. Eleven participants concluded the study. Functional independence changes were observed in relation to self-care, sphincter control, locomotion, and communication domains. Children’ “quality of life” reported by parents showed changes in functional capacity, vitality, mental health, physical and social aspects, and general state of health domains. These findings suggest that regular dance practice can underlie psychosocial adjustments in children with DS and ASD.

Understanding disease transmission is a complex problem highlighted by the COVID-19 pandemic. These studies test whether 3- to 6-year-old children in the United States use information about social interactions to predict disease transmission. Before and during COVID-19, children predicted illness would spread through close interactions. Older children outperformed younger children with no associations between task performance and pandemic experience. Children did not predict that being hungry or tired would similarly spread through close interactions. Participants include 196 three- to six-year-olds (53% girls, 47% boys; 68% White, 9% Black, 7% Asian, 6% Hispanic or Latinx), with medium-sized effects (d = .6, η p 2 = .3). These findings suggest that thinking about social interaction supports young children's predictions about illness, with noted limitations regarding children's real-world avoidance of disease-spreading behaviors.

Scientific communication poses a challenge: To clearly highlight key conclusions and implications while fully acknowledging the limitations of the evidence. Although these goals are in principle compatible, the goal of conveying complex and variable data may compete with reporting results in a digestible form that fits (increasingly) limited publication formats. As a result, authors’ choices may favor clarity over complexity. For example, generic language (e.g., “Introverts and extraverts require different learning environments”) may mislead by implying general, timeless conclusions while glossing over exceptions and variability. Using generic language is especially problematic if authors overgeneralize from small or unrepresentative samples (e.g., exclusively Western, middle-class). We present 4 studies examining the use and implications of generic language in psychology research articles. Study 1, a text analysis of 1,149 psychology articles published in 11 journals in 2015 and 2016, examined the use of generics in titles, research highlights, and abstracts. We found that generics were ubiquitously used to convey results (89% of articles included at least 1 generic), despite that most articles made no mention of sample demographics. Generics appeared more frequently in shorter units of the paper (i.e., highlights more than abstracts), and generics were not associated with sample size. Studies 2 to 4 (n = 1,578) found that readers judged results expressed with generic language to be more important and generalizable than findings expressed with nongeneric language. We highlight potential unintended consequences of language choice in scientific communication, as well as what these choices reveal about how scientists think about their data.

Individuals and cultures share some commonalities in food preferences, yet cuisines also differ widely across social groups. Eating is a highly social phenomenon; however, little is known about the judgments children make about other people’s food choices. Do children view conventional food choices as normative and consequently negatively evaluate people who make unconventional food choices? In five experiments, 5-year-old children were shown people who ate conventional and unconventional foods, including typical food items paired in unconventional ways. In Experiment 1, children preferred conventional foods and conventional food eaters. Experiment 2 suggested a link between expectations of conventionality and native/foreign status; children in the United States thought that English speakers were relatively more likely to choose conventional foods than French speakers. Yet, children in Experiments 3 and 4 judged people who ate unconventional foods as negatively as they judged people who ate canonical disgust elicitors and nonfoods, even when considering people from a foreign culture. Children in Experiment 5 were more likely to assign conventional foods to cultural ingroup members than to cultural outgroup members; nonetheless, they thought that no one was likely to eat the nonconventional items. These results demonstrate that children make normative judgments about other people’s food choices and negatively evaluate people across groups who deviate from conventional eating practices.

Picky eating in childhood is associated with children's dietary outcomes and parental feeding experiences. The Child Eating Behavior Questionnaire (CEBQ) is a frequently-used parent-report survey that measures children's eating behaviors, including picky eating. Limited work has adapted the CEBQ into a child-friendly format to measure children's ability to report directly on their own picky eating behavior. We sought to extend previous research by adapting the Food Fussiness subscale of the CEBQ into a child self-report format and measuring parent-child resemblance in scores, with children as young as 3 years. Our final sample included 3- to 10-year-old children (n = 95) and their parents, who were assessed at a local children's museum. The internal consistency of parent-report on the CEBQ FF was α = 0.9 and child-report was α = 0.7, with parent scores predicting child scores when controlling for child age and child gender. The largest difference between parent and child scores on child picky eating (with parents reporting higher scores) was for 3- to 4-year-old children. Children are able to report on their own picky eating and with age their reports converge with those of their parents, highlighting the potential benefit of collecting picky eating scores from multiple informants (parent and child). We suggest future directions for the validation and extension of this measure.

Lee and Schwarz propose grounded procedures of separation as a domain-general mechanism underlying cleansing effects. One strong test of domain generality is to investigate the ontogenetic origins of a process. Here, we argue that the developmental evidence provides weak support for a domain-general grounded procedures account. Instead, it is likely that distinct separation procedures develop uniquely for different content domains.

Assessing children’s reasoning about food, including their health knowledge and their food preferences, is an important step toward understanding how health messages may influence children’s food choices. However, in many studies, assessing children’s reasoning relies on parent report or could be susceptible to social pressure from adults. To address these limitations, the present study describes the development of a food version of the Implicit Association Test (IAT). The IAT has been used to examine children’s implicit stereotypes about social groups, yet few studies have used the IAT in other domains (such as food cognition). Four- to 12-year-olds (n = 123) completed the food IAT and an explicit card sort task, in which children assessed foods based on their perception of the food’s healthfulness (healthy vs. unhealthy) and palatability (yummy vs. yucky). Surprisingly, children demonstrated positive implicit associations towards vegetables. This pattern may reflect children’s health knowledge, given that the accuracy of children’s healthfulness ratings in the card sort task positively predicted children’s food IAT d-scores. Implications for both food cognition and the IAT are discussed.

Though parental modeling is thought to play a critical role in promoting children's healthy eating, little research has examined maternal food intake and maternal food talk as independent predictors of children's food intake. The present study examines maternal food talk during a structured eating protocol, in which mothers and their children had the opportunity to eat a series of familiar and unfamiliar vegetables and desserts. Several aspects of maternal talk during the protocol were coded, including overall food talk, directives, pronoun use, and questions. This study analyzed the predictors of maternal food talk and whether maternal food talk and maternal food intake predicted children's food intake during the protocol. Higher maternal body mass index (BMI) predicted lower amounts of food talk, pronoun use, and questions. Higher child BMI z-scores predicted more first person pronouns and more wh-questions within maternal food talk. Mothers of older children used fewer directives, fewer second person pronouns, and fewer yes/no questions. However, maternal food talk (overall and specific types of food talk) did not predict children's food intake. Instead, the most robust predictor of children's food intake during this protocol was the amount of food that mothers ate while sitting with their children. These findings emphasize the importance of modeling healthy eating through action and have implications for designing interventions to provide parents with more effective tools to promote their children's healthy eating.

Selective eating in children is commonly measured by parental report questionnaires, yet it is unknown if parents accurately estimate their child’s selective eating behavior. The objectives of this study were to test the validity and stability of two measures of selective eating using observed child behavior. Low-income mother-child dyads participated in a videotaped laboratory eating protocol at two time points (baseline: mean child age = 5.9 years; follow-up: mean child age = 8.6 years), during which they were presented with a familiar and an unfamiliar vegetable. Videos were reliably coded for child selective eating behaviors: amount consumed, child hedonic rating of vegetables, child compliance with maternal prompts to eat, latency to first bite, number of bites, and negative utterances. Mothers completed the Child Eating Behavior Questionnaire Food Fussiness (CEBQ FF) scale and the Food Neophobia Scale (FNS) at both time points. Questionnaire validity, test re-test reliability of measured behaviors, and discriminant validity of questionnaires were examined in the full sample. CEBQ FF scores and FNS scores were both inversely correlated with the quantity consumed, child hedonic rating, and compliance with prompts to eat for both familiar and unfamiliar vegetables at baseline and at follow up. CEBQ FF and FNS scores were inversely correlated with number of bites (for both foods), positively correlated with latency to first bite (for both foods), and inversely correlated with child negative utterances (for the familiar food only). Notably, FNS scores correlated with observed behavior for both familiar and unfamiliar foods, rather than demonstrating a specific association with unfamiliar foods only. This study supports the validity of the CEBQ FF and FNS in low-income early school-aged children.

The impetus for this special issue is the continuing need to identify, deconstruct, situate, analyze and re-situate the regional, national and internecine conflicts which drive the forced migration...

Past research finds that monolingual and bilingual children prefer native speakers to individuals who speak in unfamiliar foreign languages or accents. Do children in bilingual contexts socially distinguish among familiar languages and accents and, if so, how do their social preferences based on language and accent compare? The present studies tested whether 5- to 7- year-old children in two bilingual contexts in the United States demonstrate social preferences among the languages and accents that are present in their social environments. We compared children’s preferences based on language (i.e., English versus their other native language) and their preferences based on accent (i.e., English with a native accent versus English with a non-native, yet familiar, accent). In Experiment 1, children attending a French immersion school demonstrated no preference between English and French speakers, but preferred American-accented English to French-accented English. In Experiment 2, bilingual Korean-American children demonstrated no preference between English and Korean speakers, but preferred American-accented English to Korean-accented English. Across studies, bilingual children’s preferences based on accent (i.e., American-accented English over French- or Korean-accented English) was not related to their own language dominance. These results suggest that children from diverse linguistic backgrounds demonstrate social preferences for native-accented speakers. Implications for understanding the potential relation between social reasoning and language acquisition are discussed.

Data file for manuscript review. Includes data and variable names. Race and income have been omitted for confidentiality but will be made available upon request.

Can brief messages about health influence children’s consumption of identical foods? Across a series of studies, we manipulated children’s consumption of identical foods (fruit sauces) by pairing those foods with brief messages about each food’s health status. What initially appeared to be a preference for foods described as healthy among 5–6-year-old children (Studies 1–2) actually reflected a preference for alternatives to foods described as unhealthy (Studies 3–5), including comparison foods that were described with negative or neutral content. Although the two foods on each trial were identical, children consistently ate more of the alternative to a food described as unhealthy. Similar effects were observed among 8–9-year-old children (Study 6). These results demonstrate that children’s eating behavior is affected by messages they receive from other people, including messages about health. Further, these studies reveal basic psychological mechanisms that contribute to children’s choices among foods, which could lead to effective interventions in the food domain.

The present research assessed 5- to 10-year-old Indian children’s attention to social category information and status when evaluating the nationality and characteristics of novel individuals. In Study 1, children chose which of two targets was “more Indian” (with the option to choose “both”). Targets varied on three social dimensions: Skin Tone (White, Lighter-skinned South Asian, Darker-skinned South Asian), Religion (Hindu, Muslim), and Language (Tamil [local state language], Hindi [India’s lingua franca], British-accented-English, Indian-accented-English). Children reliably chose Lighter-skinned South Asian, Hindu, and Tamil-speaking targets as more Indian. In Study 2, focusing on the language contrasts from Study 1, we replicated our nationality findings and extended them to person judgments (kindness, intelligence, and leadership). Children chose Tamil speakers as more “Indian,” and “kind,” Tamil and British-accented-English speakers as more “intelligent,” and British-accented-English speakers as “better leader[s].” Children’s responses reflected attention to markers of social familiarity, representativeness, and status.

Encouraging children to participate in food preparation is recommended by pediatric guidelines and has been included in public health interventions. However, little is known about whether the act of preparing a food specifically increases children's intake of that food, nor is it known whether this effect might differ for healthy and familiar unhealthy foods. The present study examines whether 5- to 7-year-old children eat more of a food they prepared themselves compared to the same food prepared by someone else. Children participated in a laboratory study in which they prepared either a salad or a dessert and then had the opportunity to eat the food they prepared and/or a nearly identical food prepared by someone else. We found that children ate more of a food they prepared themselves, but no significant difference was observed in children's ratings of each food. In addition to eating more healthy foods they prepared themselves, children ate more unhealthy foods they prepared themselves, including familiar and well-liked desserts. More specific recommendations are needed if the goal of involving children in food preparation is to promote health.

Adults implicitly judge people from certain social backgrounds as more "American" than others. This study tests the development of children's reasoning about nationality and social categories. Children across cultures (White and Korean American children in the United States, Korean children in South Korea) judged the nationality of individuals varying in race and language. Across cultures, 5- to 6-year-old children (N = 100) categorized English speakers as "American" and Korean speakers as "Korean" regardless of race, suggesting that young children prioritize language over race when thinking about nationality. Nine- and 10-year-olds (N = 181) attended to language and race and their nationality judgments varied across cultures. These results suggest that associations between nationality and social category membership emerge early in life and are shaped by cultural context.

Does contextual information about disgust influence children's food consumption and subjective experience of taste? Three- to eight-year-old children (N = 60) were presented with two identical foods, yet children were led to believe that one food had been contaminated by sneezing and licking, while the other was clean. When given the opportunity to eat the foods, 5- to 8-year-old children consumed more clean food and rated the clean food's taste more positively; younger children did not distinguish between the foods. The relation between contamination and subjective taste held even among children who ate both foods and had direct evidence that they were identical. These data indicate that children's consumption behavior and food preferences are influenced by information external to foods themselves.

Eating is inherently social and cultural, and as food generalists, children face a difficult task in learning to consume a diet that is both culturally and biologically appropriate. In this chapter, we provide an overview of children's early thinking and behavior in the food domain, focusing especially on the important role that social learning plays in guiding children's knowledge, preferences, and choices. An additional goal of the chapter is to highlight the role that carefully controlled laboratory studies can play in the quest to explain—and potentially improve—children's eating.

How does social information affect the perception of taste early in life? Does mere knowledge of other people’s food preferences impact children’s own experience when eating? In Experiment 1, 5- and 6-year-old children consumed more of a food described as popular with other children than a food that was described as unpopular with other children, even though the two foods were identical. In Experiment 2, children ate more of a food described as popular with children than a food described as popular with adults. Experiment 3 tested whether different perceptual experiences of otherwise identical foods contributed to the mechanisms underlying children’s consumption. After sampling both endpoints of a sweet-to-sour range (applesauce with 0 mL or 5mL of lemon juice added), children were asked to taste and categorize applesauce samples with varying amounts of lemon juice added. When classifying ambiguous samples that were near the midpoint of the range (2 mL and 3 mL), children were more likely to categorize popular foods as sweet as compared to unpopular foods. Together, these findings provide evidence that social information plays a powerful role in guiding children’s consumption and perception of foods. Broader links to the sociality of food selection are discussed.

Recombinant proteins are of great commercial and scientific interest. However, most current production methods using mammalian cells involve the time- and labor-intensive step of creating stable cell lines. Although production methods based on transient gene expression could offer a significant improvement, transient transfection is currently still limited by low titers and low specific productivity compared to stable cell lines. To overcome these bottlenecks, we have explored the use of various growth factors to enhance specific productivity and titers in the context of transient gene expression. For that purpose, several growth factors were cloned and screened for their effect on transient gene expression in HEK293E and CHO-DG44 cells. In particular, acidic fibroblast growth factor (aFGF) was able to increase specific productivity by 60% and recombinant protein titers by 80% in HEK293E cells, while FGF9 increased titers by 250% in CHO-DG44 cells.

A new scalable reactor was developed by applying a novel mixing principle that allows the large-scale cultivation of mammalian cells simply with surface aeration using air owing to increased liquid-gas transfer compared to standard stirred-tank bioreactors. In the cylindrical vessels (50 mL-1500 L) with a helical track attached to the inside wall, the liquid moved upward onto the track as the result of orbital shaking to increase the liquid-gas interface area significantly. This typically resulted in a 5-10-fold improvement in the volumetric mass transfer coefficient (k(L)a). In a 1500-L helical track vessel with a working volume of 1000 L, a k(L)a of 10h(-1) was obtained at a shaking speed of 39 rpm. Cultivations of CHO cells in a shaken 55-L helical track bioreactor resulted in improved cell growth profiles compared to control cultures in standard systems. These results demonstrated the possibility of using these new bioreactors at scales of 1000 L or more.

Study Design: Case report. Objectives: To describe the first use of intravenous (IV) ketamine as the sole agent in a patient-controlled analgesic delivery system (ie PCA) in a patient with cervical syringomyelia. Setting: A tertiary-care university teaching hospital in New York City. Methods: A 41-year-old tetraplegic female on high-dose opioids suffering from intractable dysesthetic central pain received her best pain relief from a low-dose ketamine infusion after failing trials with multiple neuropathic medications. After several weeks of titrating her infusion rate up and down, she was switched to an IV ketamine PCA device. Results: The patient was maintained on an IV ketamine PCA for almost 1 year under the following settings: 2.7 mg/h basal rate; 2.7 mg/h demand dose; 15 min lockout period. Although she continues to report some pain, it has dramatically decreased since the ketamine PCA was instituted, enabling us to significantly reduce her opioid dosage. Conclusions: Ketamine PCA may be a viable treatment option in patients suffering from intractable central pain. The rationale for this treatment, along with dosing guidelines and possible drawbacks, is discussed.

Non-intrusive flow visualization using photochromic dye activation is applied to study the near-wall region around individual and coalescing pairs of Taylor bubbles rising in stagnant kerosene in a vertical 25.6 mm ID tube. Instantaneous velocity profile measurements have been performed in the liquid film flowing past the rising Taylor bubble. A simple predictive procedure for estimating film velocity is successfully implemented. New and interesting phenomena are observed primarily in the wake region that are of significance to slug flow modeling.

Adults evaluate others based on their speech, yet little is known of the developmental trajectory by which accent attitudes are acquired. Here we investigate the development of American children's attitudes about Northern- and Southern-accented American English. Children in Illinois (the “North”) and Tennessee (the “South”) evaluated the social desirability, personality characteristics, and geographic origins of Northern- and Southern-accented individuals. Five- to 6-year-old children in Illinois preferred the Northern-accented speakers as potential friends, yet did not demonstrate knowledge of any stereotypes about the different groups; 5–6-year-old children in Tennessee did not show a preference towards either type of speaker. Nine- to 10-year-old children in both Illinois and Tennessee evaluated the Northern-accented individuals as sounding “smarter” and “in charge”, and the Southern-accented individuals as sounding “nicer.” Thus, older children endorse similar stereotypes to those observed in adulthood. These accent attitudes develop in parallel across children in different regions and reflect both positive and negative assessments of a child's own group.

For more than 20 years, primary prevention of coronary heart disease has included strategies intended to improve overall serum lipid concentrations among youths.To examine trends in lipid concentrations among youths from 1988-1994 through 2007-2010.Cross-sectional analysis of serum lipid concentrations among 16,116 youths aged 6 to 19 years who participated in the nationally representative National Health and Nutrition Examination Survey during 3 time periods: 1988-1994, 1999-2002, and 2007-2010.Among all youths, mean serum total cholesterol (TC), non-high-density lipoprotein cholesterol (non-HDL-C), high-density lipoprotein cholesterol (HDL-C); and among adolescents only, low-density lipoprotein cholesterol (LDL-C) and geometric mean triglyceride levels. Trends in adverse lipid concentrations are reported for TC levels of 200 mg/dL and greater, non-HDL-C levels of 145 mg/dL and greater, HDL-C levels of less than 40 mg/dL, LDL-C levels of 130 mg/dL and greater, and triglyceride levels of 130 mg/dL and greater.Among youths aged 6 to 19 years between 1988-1994 and 2007-2010, there was a decrease in mean TC (from 165 mg/dL [95% CI, 164-167] to 160 mg/dL [95% CI, 158-161]; P.001) and a decrease in the prevalence of elevated TC (from 11.3% [95% CI, 9.8%-12.7%] to 8.1% [95% CI, 6.7%-9.5%]; P = .002). Mean HDL-C significantly increased between 1988-1994 and 2007-2010, but the prevalence of low HDL-C did not change. Mean non-HDL-C and prevalence of elevated non-HDL-C both significantly decreased over the study period. In 2007-2010, 22% (95% CI, 20.3%-23.6%) of youths had either a low HDL-C level or high non-HDL-C, which was lower than the 27.2% (95% CI, 24.6%-29.7%) in 1988-1994 (P = .001). Among adolescents (aged 12-19 years) between 1988-1994 and 2007-2010, there was a decrease in mean LDL-C (from 95 mg/dL [95% CI, 92-98] to 90 mg/dL [95% CI, 88-91]; P = .003) and a decrease in geometric mean triglycerides (from 82 mg/dL [95% CI, 78-86] to 73 mg/dL [95% CI, 70-76]; P.001). Prevalence of elevated LDL-C and triglycerides between 1988-1994 and 2007-2010 also significantly decreased.Between 1988-1994 and 2007-2010, a favorable trend in serum lipid concentrations was observed among youths in the United States but almost 1 in 10 had elevated TC in 2007-2010.

Three experiments investigated 5- to 6-year-old monolingual English-speaking American children's sociolinguistic evaluations of others based on their accent (native, foreign) and social actions (nice, mean, neutral). In Experiment 1, children expressed social preferences for native-accented English speakers over foreign-accented speakers, and they judged the native-accented speakers to be 'American.' In Experiments 2 and 3, the accented speakers were depicted as being nicer than the relatively meaner native speakers. Children's social preferences and judgments of others' personalities varied as a function of behavior; in particular, children disliked individuals who committed negative social actions. In contrast, children's judgments of nationality hinged exclusively on accent; across all conditions, children evaluated native-accented English speakers to be 'American,' regardless of whether they were nice or mean. These findings contribute to an understanding of the nature of children's reasoning about language as a social category and have implications for future research investigating children's thinking about language as a marker of national group identity.

A series of experiments investigated the effect of speakers’ language, accent, and race on children’s social preferences. When presented with photographs and voice recordings of novel children, 5-year-old children chose to be friends with native speakers of their native language rather than foreign-language or foreign-accented speakers. These preferences were not exclusively due to the intelligibility of the speech, as children found the accented speech to be comprehensible, and did not make social distinctions between foreign-accented and foreign-language speakers. Finally, children chose same-race children as friends when the target children were silent, but they chose other-race children with a native accent when accent was pitted against race. A control experiment provided evidence that children’s privileging of accent over race was not due to the relative familiarity of each dimension. The results, discussed in an evolutionary framework, suggest that children preferentially evaluate others along dimensions that distinguished social groups in prehistoric human societies.

One hundred patients were randomly allocated to receive saline or amrinone, 0.75 mg.kg-1, ten minutes before separation from cardiopulmonary bypass (CPB) after elective coronary artery bypass grafting, in order to determine the effects of this agent on haemodynamic variables and O2 utilization. Anaesthesia and CPB were managed in a standard fashion. Before induction of anaesthesia, at pericardiotomy, then at 1, 10, 20 and 30 min after CPB, haemodynamic profiles, haematocrit, and O2 saturation of arterial and mixed venous blood were measured. Incremental doses of ephedrine or phenylephrine, or an infusion of norepinephrine with phentolamine were administered when required. The groups were demographically similar and surgical variables were also similar. Haemodynamic measurements were similar between groups at all times; however, a higher dose of phenylephrine was given immediately before weaning from CPB in the amrinone group, and more patients in this group received phenylephrine in the first 30 min after CPB. Mixed venous saturation (SvO2) was higher in the amrinone patients at all times after CPB, leading to lower calculated oxygen consumption (VO2) (P less than 0.05). Insufficient dosage may explain the lack of haemodynamic effect, while possible reasons for the higher SvO2 and lower VO2 are either reduced whole body VO2 or peripheral shunting.

Cylindrical containers agitated by orbital shaking are being developed as simple and cost-effective bioreactor systems for the cultivation of mammalian cells. Here the oxygen transfer capacities of containers with nominal volumes from 50 mL to 2000 L were determined, and the operating parameters influencing oxygen transfer were investigated. In general, the shaking speed necessary for efficient oxygen transfer diminished as the container size increased. At shaking speeds suitable for the growth of shear-sensitive cells, kLa values between 10 and 30 h−1 were typically achieved in small-scale (

Interfacial area was measured in cocurrent, gas-liquid upward flow through a vertical, 2.54 cm I.D. and 2.67 m long tube by absorption of CO2 diluted in air into aqueous sodium hydroxide solution. Void fraction and pressure drop were also measured for superficial liquid velocities up to 1.3 m/s. Although the interfacial area exhibited a maximum and minimum with increasing superficial gas velocities at relatively high liquid flow rates as in previous experiments, several significant differences were found. Since the dissipation parameters proposed in the past to correlate interfacial area were found to be less than satisfactory, a new empirical relation is proposed, which can correlate most of the present data within ± 20%. On a mcsure la surface intcrfaciale en ecoulement ascendant gaz-liquide a cocourant dans un tube de 234 cm de diametre interieur et de 2,67 m de long, par absorption de CO2 dilue dans l'air dans une solution d'hydroxyde de sodium aqueux. On a egalement mesure la fraction de vide et la perte de charge pour des vitesses liquides superficielles allant jusqu'a 1.3 m/s. Bien que la surface interfaciale montre un maximum et un minimum avec l'accroissement des vitesses de gaz superficielles a des debits liquides relativement eleves, plusieurs differences importantes ont ete remarquees. Ixs parametres de dissipation proposes dans le passe pour la correation de la surface interfaciale s'etant averes insatisfaisants, on propose une nouvelle relation enipirique, capable de correler la plupart des donnCes actuelles avec une erreur inferieure a 20%.

The use of mammalian cells for research and for numerous applications could be facilitated significantly if pausing, i.e. reduction and reactivation of cellular activity, could be executed at will without negative effects. Preferably, pausing should be possible at any phase of a cell culture and at any scale of operations. In this communication we report our first observations to pause cell cultures by temperature reduction. Little information is available in the literature on cold exposure of mammalian cells (1). For this study, we took advantage of the availability of stable GFP-transfected CHO cells whose biomass in culture could be fast and noninvasively monitored by a standard fluorescence reader (2).

Methods for the transfection of DNA into mammalian cells for transient gene expression were initially developed about 30 years ago. The main purpose was and still remains the molecular analysis of the cell’s response to the exogenous protein. The use of transient gene expression for industrial purposes began in 1991 when Bennett and coworkers identified by alanine-scanning mutagenesis several mutants of human tissue-plasminogen activator (rtPA) that were eventually shown to be of higher medical potency than the wild-type protein. One of the molecules, now an approved thrombolytic “TNKase™”, had a dramatically extended half-life in patients, a high fibrin-specificity, and was insensitive to the inhibitor of plasminogen-activator PAI. The identification of improved mutants was based on more than 40,000 transient gene expression experiments (Wurm et al. unpublished) executed during the course of one year using calcium-phosphate transfections in human embryonic kidney (HEK) 293 cells (Gorman et al 1990).

The arthritides discussed are presented with many drug therapies because of a not fully understood and accepted cause of the disease process. Similarly, the drug therapies offered are diverse and have mechanisms that are not always fully understood. Because of the destruction and disability the arthritides are capable of and the realization that destruction may be reversible early in the course of the disease, physicians are initiating earlier, aggressive treatment with DMARDs. Long-term outcome studies of the early institution of DMARDs, combination therapies, and newer medications need to be evaluated to produce the most efficient treatment regimens.

The psychoneuroimmunology of panic disorder is relatively unexplored. Alterations within brain stress systems that secondarily influence the immune system have been documented. A recent report indicated elevations of serotonin (5-HT) and ganglioside antibodies in patients with primary fibromyalgia, a condition with documented associations with panic disorder. In line with our interest in dysregulated 5-HT systems in panic disorder (PD), we wished to assess if antibodies directed at the 5-HT system were elevated in patients with PD in comparison to healthy volunteers. Sixty-three patients with panic disorder and 26 healthy volunteers were diagnosed by the SCID. Employing ELISA, we measured anti-5-HT and 5-HT anti-idiotypic antibodies (which are directed at 5-HT receptors). To include all subjects in one experiment, three different batches were run during the ELISA. Plasma serotonin anti-idiotypic antibodies: there was a significant group effect [patients > controls (p = .007)] and batch effect but no interaction. The mean effect size for the three batches was .76. Following Z-score transformation of each separate batch and then combining all scores, patients demonstrated significantly elevated levels of plasma serotonin anti-idiotypic antibodies. Neither sex nor age as covariates affected the significance of the results. There was a strong correlation between anti-serotonin antibody and serotonin anti-idiotypic antibody measures. Plasma anti-serotonin antibodies: there was a significant diagnosis effect [patients > controls (p = .037)]. Mean effect size for the three batches was .52. Upon Z-score transformation, there was a diagnosis effect with antibody elevations in patients. Covaried for sex and age, the result falls below significance to trend levels. The data raise the possibility that psychoimmune dysfunction, specifically related to the 5-HT system, may be present in PD. Potential interruption of 5-HT neurotransmission through autoimmune mechanisms may be of pathophysiologic significance in certain patients with panic disorder. It remains to be demonstrated if the peripheral autoimmunity is representative of CNS 5-HT neuronal alterations. Replication appears warranted.

Subluxation of the peroneal tendons is a rare injury that is often misdiagnosed. This injury is usually related to and often accompanied by lateral ankle ligament sprains. A comprehensive review of the literature, as well as two cases of surgically treated subluxing peroneal tendons will be presented. Anatomy, etiology, and diagnosis will be discussed. Both conservative and surgical treatment methodology will be discussed in detail.