26 results on '"M. Cliffe"'
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2. The ELFIN Mission
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V. Angelopoulos, E. Tsai, L. Bingley, C. Shaffer, D. L. Turner, A. Runov, W. Li, J. Liu, A. V. Artemyev, X.-J. Zhang, R. J. Strangeway, R. E. Wirz, Y. Y. Shprits, V. A. Sergeev, R. P. Caron, M. Chung, P. Cruce, W. Greer, E. Grimes, K. Hector, M. J. Lawson, D. Leneman, E. V. Masongsong, C. L. Russell, C. Wilkins, D. Hinkley, J. B. Blake, N. Adair, M. Allen, M. Anderson, M. Arreola-Zamora, J. Artinger, J. Asher, D. Branchevsky, M. R. Capitelli, R. Castro, G. Chao, N. Chung, M. Cliffe, K. Colton, C. Costello, D. Depe, B. W. Domae, S. Eldin, L. Fitzgibbon, A. Flemming, I. Fox, D. M. Frederick, A. Gilbert, A. Gildemeister, A. Gonzalez, B. Hesford, S. Jha, N. Kang, J. King, R. Krieger, K. Lian, J. Mao, E. McKinney, J. P. Miller, A. Norris, M. Nuesca, A. Palla, E. S. Y. Park, C. E. Pedersen, Z. Qu, R. Rozario, E. Rye, R. Seaton, A. Subramanian, S. R. Sundin, A. Tan, W. Turner, A. J. Villegas, M. Wasden, G. Wing, C. Wong, E. Xie, S. Yamamoto, R. Yap, A. Zarifian, and G. Y. Zhang
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- 2020
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3. Correction to: The ELFIN Mission
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A. Gonzalez, A. Flemming, Eric Grimes, D. Branchevsky, A. Subramanian, D. M. Frederick, K. Hector, D. Hinkley, W. Greer, J. Mao, E. McKinney, A. Tan, A. Norris, A. J. Villegas, E. S. Y. Park, C. Shaffer, M. Cliffe, C. Wong, M. Anderson, R. Krieger, K. Lian, A. V. Artemyev, Ian Fox, B. Hesford, P. Cruce, G. Chao, C. E. Pedersen, J. Artinger, R. Rozario, M. Allen, Robert J. Strangeway, Richard E. Wirz, K. Colton, B. W. Domae, R. Seaton, Wen Li, N. Chung, J. Asher, J. B. Blake, Susmit Jha, M. Chung, A. Palla, D. Leneman, M. Wasden, R. Yap, E. Rye, V. A. Sergeev, D. Depe, A. Gildemeister, Drew Turner, Catherine E. Costello, S. R. Sundin, C. Wilkins, Emmanuel Masongsong, L. Fitzgibbon, A. Gilbert, S. Eldin, C. L. Russell, L. Bingley, R. Caron, G. Y. Zhang, Z. Qu, E. Xie, G. Wing, J. King, Xiao-Jia Zhang, R. Castro, E. Tsai, Jiang Liu, Vassilis Angelopoulos, W. Turner, M. Arreola-Zamora, Andrei Runov, N. Kang, M. Nuesca, S. Yamamoto, J. P. Miller, A. Zarifian, Yuri Shprits, N. Adair, M. R. Capitelli, and M. J. Lawson
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Final version ,Planetary science ,Space and Planetary Science ,Art history ,Astronomy and Astrophysics ,Space (commercial competition) - Abstract
Correction to: Space Sci. Rev. (2020) 216: 103 Affiliation 2 in this article is the wrong affiliation that instead should read: “Institute of Geophysics and Planetary Physics, University of California, Los Angeles, CA, USA” which should be regarded as the final version by the reader.
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- 2020
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4. Karyomapping—a comprehensive means of simultaneous monogenic and cytogenetic PGD: comparison with standard approaches in real time for Marfan syndrome
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Karen Sage, Gary L. Harton, Christian S. Ottolini, Alan H. Handyside, Senthil A Natesan, Darren K. Griffin, Nabeel A. Affara, Alan R. Thornhill, Dagan Wells, Kerry M. Cliffe, Jon Taylor, and M. Konstantinidis
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medicine.medical_specialty ,Biopsy ,Aneuploidy ,Locus (genetics) ,Single-nucleotide polymorphism ,Fertilization in Vitro ,Biology ,Preimplantation genetic diagnosis ,Polymorphism, Single Nucleotide ,Marfan Syndrome ,Cytogenetics ,Pregnancy ,Obstetrics and Gynaecology ,Gene duplication ,Genetics ,medicine ,Humans ,Genetics(clinical) ,Allele ,Preimplantation Diagnosis ,Genetics (clinical) ,Haplotype ,Obstetrics and Gynecology ,General Medicine ,medicine.disease ,Haplotypes ,Reproductive Medicine ,Female ,Developmental Biology - Abstract
Preimplantation genetic diagnosis (PGD) of single gene defects by genetic analysis of single or small numbers of cells biopsied from in vitro fertilization (IVF) embryos is clinically well-established. Targeted haplotyping by multiplex fluorescent polymerase chain reaction (PCR) of closely linked or intragenic short tandem repeat (STR) markers combined with direct mutation detection improves the accuracy of single cell analysis significantly and minimizes potential errors caused by undetected allele dropout (ADO) or contamination [1]. Allele dropout refers to the failure of one of the two alleles of a heterozygous locus to amplify. This makes a heterozygous cell appear homozygous at the affected locus, potentially leading to misdiagnosis. Furthermore, using high order multiplex protocols, this approach has been extended to multiple loci, including analysis of the Human leukocyte antigen (HLA) region for selection of embryos tissue matched to existing sick children and diagnosis of translocation chromosome imbalance [2–4]. However, the development of patient, disease or locus-specific protocols, and testing with single cells, is time-consuming and labour intensive. Also, this targeted approach only provides limited information on chromosome aneuploidy, which is recognized to be a major cause of IVF failure and pregnancy loss. As an alternative, we developed, “Karyomapping”—genome wide parental haplotyping using high density single nucleotide polymorphism (SNP) genotyping. Karyomapping provides a comprehensive method for linkage-based diagnosis of any single gene defect [5]. Genotyping of the parents and a close relative of known disease status, to phase informative SNP loci, eliminates the need for customized test development and, as Karyomapping defines four sets of SNP markers for each of the parental chromosomes, it allows simultaneous high-resolution molecular cytogenetic analysis. Thus, meiotic trisomies, including their parental origin, can be identified by the presence of both haplotypes from one parent in segments of the chromosome, resulting from the inheritance of two chromosomes with different patterns of recombination. Moreover, monosomies or deletions can be identified by the absence of either chromosome haplotype from the parent of origin [5]. Mitotic chromosome duplication, which can arise through malsegregation of chromosomes in the cleavage divisions following fertilization, cannot be detected by Karyomapping per se, since the sequence of both chromosomes is identical. However, chromosome duplications may be clinically less significant, since they are often associated with poor morphology and developmental arrest. In the past we have demonstrated that Karyomapping could be used for the detection of cystic fibrosis status in single cells [5]. Here we provide proof of principle for the widespread clinical application of Karyomapping, first by adapting the protocol for clinical use in a regular PGD timeframe (24 h) and secondly by detection of the autosomal dominant condition Marfan syndrome. Performing Karyomapping “as if in a clinical setting” for confirmation of results of an existing PGD case provides strong evidence of the applicability of Karyomapping and, in this case, led to a twin birth.
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- 2015
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5. SCUBA-2 arrays to system interfaces
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William Parkes, T. Baillie, M. Cliffe, Maureen A. Ellis, J. Molnar, Kent D. Irwin, L. Ferreira, Wayne S. Holland, Dan Bintley, A. D. Ruthven, Gene C. Hilton, M. Fich, C. D. Reintsema, Jan Kycia, T. Hodson, H. McGregor, M. J. MacIntosh, Fred Gannaway, Xiaofeng Gao, Camelia Dunare, Adam Woodcraft, J. N. Ullom, Jonathan G. Terry, William B. Doriese, D. Audley, M. Halpern, T. Peacocke, Ian Robson, Anthony Walton, G. Mitchell, Carole Tucker, David Atkinson, Matthew Joseph Griffin, Peter A. R. Ade, L. R. Vale, David C. Gostick, B. Burger, Ian Walker, D. Kelly, Eric F Schulte, Tom Stevenson, D. Naylor, W. D. Duncan, Stewart Smith, Rashmikant V. Sudiwala, Alan M. Gundlach, P. Bastien, and I. Smith
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Physics ,business.industry ,Stray light ,Bolometer ,Electrical engineering ,General Medicine ,Multiplexer ,law.invention ,Interferometry ,Optics ,Pathfinder ,law ,Electromagnetic shielding ,Measuring instrument ,Astronomical interferometer ,business - Abstract
Submillimeter common user bolometer array (SCUBA)-2 is a wide field sub-mm bolometer camera designed to replace the existing SCUBA instrument on the JCMT in Hawaii. It will be many hundreds of times faster in large area mapping than SCUBA and will also go deeper in a single frame. It will enable the many discoveries of SCUBA to be followed up with deep systematic surveys and help act as a pathfinder for the ALMA interferometer. The key technologies for making the arrays have been demonstrated and will be put together to fabricate the first prototype later this year (2003). The wide field nature of the SCUBA-2 bolometer camera, combined with the diffraction limit at sub-mm wavelengths, leads to physically large focal planes where the issues of stray light control, magnetic shielding, and electrical, thermal and mechanical connection must be carefully addressed in order to realise a successful instrument. We describe the solutions we have adopted for these problem areas.
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- 2004
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6. Impact on offspring methylation patterns of maternal gestational diabetes mellitus and intrauterine growth restraint suggest common genes and pathways linked to subsequent type 2 diabetes risk
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Miguel Constância, Philippa M. Prentice, Claire R. Quilter, David B. Dunger, Julien Bauer, Latasha Nelson, Kerry M. Cliffe, Nabeel A. Affara, William L. Lowe, Benjamin M. Skinner, Ken K. Ong, and Wendy N. Cooper
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Adult ,Blood Glucose ,Male ,Risk ,medicine.medical_specialty ,Offspring ,Birth weight ,Type 2 diabetes ,Biology ,Weight Gain ,Biochemistry ,Fetal Development ,Young Adult ,Pregnancy ,Internal medicine ,Diabetes mellitus ,Genetics ,medicine ,Birth Weight ,Humans ,Molecular Biology ,DNA Methylation ,medicine.disease ,Gestational diabetes ,Low birth weight ,Diabetes, Gestational ,Endocrinology ,Differentially methylated regions ,Diabetes Mellitus, Type 2 ,Hyperglycemia ,Female ,medicine.symptom ,Biotechnology - Abstract
Size at birth, postnatal weight gain, and adult risk for type 2 diabetes may reflect environmental exposures during developmental plasticity and may be mediated by epigenetics. Both low birth weight (BW), as a marker of fetal growth restraint, and high birth weight (BW), especially after gestational diabetes mellitus (GDM), have been linked to increased risk of adult type 2 diabetes. We assessed DNA methylation patterns using a bead chip in cord blood samples from infants of mothers with GDM (group 1) and infants with prenatal growth restraint indicated by rapid postnatal catch-up growth (group 2), compared with infants with normal postnatal growth (group 3). Seventy-five CpG loci were differentially methylated in groups 1 and 2 compared with the controls (group 3), representing 72 genes, many relevant to growth and diabetes. In replication studies using similar methodology, many of these differentially methylated regions were associated with levels of maternal glucose exposure below that defined by GDM [the Hyperglycemia and Adverse Pregnancy Outcome (HAPO) study] or were identified as changes observed after randomized periconceptional nutritional supplementation in a Gambian cohort characterized by maternal deprivation. These studies provide support for the concept that similar epigenetic modifications may underpin different prenatal exposures and potentially increase long-term risk for diseases such as type 2 diabetes.
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- 2014
7. Immunosuppressive therapy for peripartum-type spontaneous coronary artery dissection: Case report and review
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P T Koller, C M Cliffe, and D J Ridley
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medicine.medical_specialty ,medicine.diagnostic_test ,business.industry ,Vascular disease ,General Medicine ,medicine.disease ,Sudden cardiac death ,Surgery ,Dissection ,Aneurysm ,Left coronary artery ,medicine.artery ,Angiography ,medicine ,Myocardial infarction ,Cardiology and Cardiovascular Medicine ,business ,Postpartum period - Abstract
Background: A 35-year-old postpartum woman presented with myocardial infarction (MI) due to spontaneous coronary artery dissection (SCAD). In addition to conventional medical therapy, she was treated with immunosuppressive agents (prednisone and cytoxan) because of five noncontiguous coronary dissections, surgical inoperability, and postinfarction angina. Repeat angiography 94 days later demonstrated angiographically normal vessels. Methods: A literature review of 42 additional cases of peripartum SCAD was performed, and the results were compared with this patient. Results: Mortality was 48.8% (21/43). Sudden cardiac death was the initial presentation in 27.9% (12/43) of cases. The left coronary artery was involved in 78.6% (44/56) of dissections with left main segment dissection in 10 cases. In women who survived > 24 h post infarction, recurrent MI, usually due to a second coronary dissection, occurred in 20.8% (5/24). Histopathologic studies have often shown periadventitial inflammation (80%, 16/20), in which eosinophils predominate (68.8%, 11/16), and may be linked to the medial degeneration often found in these cases. Conclusions: The common observation of eosinophilic periadventitial inflammation suggests a role in the pathophysiology of this rare, yet serious condition. Aggressive immunosuppressive therapy in this case of multivessel dissection resulted in spontaneous angiographic healing of all lesions.
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- 1998
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8. Analysis of the non-recombining Y chromosome defines polymorphisms in domestic pig breeds: ancestral bases identified by comparative sequencing
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K M, Cliffe, A E, Day, M, Bagga, K, Siggens, C R, Quilter, S, Lowden, H A, Finlayson, C J, Palgrave, N, Li, L, Huang, S C, Blott, and C A, Sargent
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Male ,China ,Sus scrofa ,Sequence Analysis, DNA ,Breeding ,Polymorphism, Single Nucleotide ,Sex-Determining Region Y Protein ,Europe ,Haplotypes ,Y Chromosome ,Animals ,Nucleic Acid Amplification Techniques ,Phylogeny ,DNA Primers - Abstract
Sequences from 20 amplicons representing nine different loci and 11369bp from the short arm of the pig Y chromosome were compared using pools of DNA from different European and Chinese breeds. A total of 33 polymorphic sites were identified, including five indels and 28 single nucleotide polymorphisms (SNPs). Three high frequency SNPs within the coding regions of SRY were further analysed across 889 males representing 25 European and 25 Asian breeds or Lines, plus a European Line of Meishan. Two haplotypes seen to be associated with 'European' or 'Chinese' origin in the initial SNP discovery phase were found to be the most common in their respective groups of breeds in a more detailed genotyping study. Two further SRY haplotypes are relatively rare. One was found exclusively within Tamworth, at low frequency in Retinto, and in three Chinese breeds (Huai, Sahwutou and Xiaomeishan). The other uncommon haplotype is found exclusively in Bamajiang, two further Chinese breeds (Hangjiang Black and Longling) and two European rare breeds (Mangalica and Linderödssvin), but appears based on comparison with other suids to represent an ancestral sequence.
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- 2010
9. Molecular biology of rice tungro viruses: evidence for a new retroid virus
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R. Hull, M. C. Jones, I. Dasgupta, J. M. Cliffe, C. Mingins, G. Lee, and J. W. Davies
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- 2008
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10. SCUBA-2: A large-format CCD-style imager for submillimeter astronomy
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Cynthia L. Hunt, Dan Bintley, Xiaofeng Gao, Adam Woodcraft, Kent D. Irwin, William Parkes, Wayne S. Holland, Maureen A. Ellis, Peter A. R. Ade, Joel N. Ullom, Ian Walker, Anthony J. Walton, Gene C. Hilton, Carl D. Reintsema, Rashmikant V. Sudiwala, I. Smith, David Atkinson, David A. Naylor, Camelia Dunare, H. McGregor, Gayle F Mitchell, T. Hodson, Fred Gannaway, Ian Robson, Jan Kycia, David Montgomery, Leila R. Vale, Mark Halpern, Pierre Bastien, M. Fich, M. J. MacIntosh, Giampaolo Pisano, Randy Doriese, M. Cliffe, David C. Gostick, Michael D. Audley, William Duncan, and D. Kelly
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Physics ,instrumentation ,detector arrays ,media_common.quotation_subject ,Bolometer ,Astronomy ,Large format ,Astrophysics ,Galaxy ,Submillimetre astronomy ,law.invention ,Sky ,law ,Astronomical interferometer ,Galaxy formation and evolution ,Transition edge sensor ,TES detectors ,Millimetre wave astronomy, instrumentation, TES detectors, detector arrays ,Millimetre wave astronomy ,media_common - Abstract
We describe the capabilities of SCUBA-2, the first CCD-like imager for submillimeter astronomy, and the technologies that make it possible. Unlike previous detectors using discrete bolometers, SCUBA-2 has two dc-coupled, monolithic arrays with a total of ~10,000 bolometers. SCUBA-2’s absorber-coupled pixels use superconducting transition edge sensors operating at ~ 120mK for photon-noise limited performance and a SQUID time-domain multiplexer for readout. It will offer simultaneous imaging of an 8 × 8 arcmin field of view at wavelengths of 850 μm and 450 μm. SCUBA-2 is expected to have a huge impact on the study of galaxy formation and evolution in the early Universe as well as star and planet formation in our own Galaxy. Mapping the sky to the same S/N up to 1000 times faster than SCUBA, SCUBA-2 will also act as a pathfinder for submillimeter interferometers such as ALMA. SCUBA-2 will begin operation on the JCMT in 2006.
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- 2007
11. SCUBA-2: A large-format TES array for submillimetre astronomy
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David C. Gostick, Xiaofeng Gao, Maureen A. Ellis, Adam Woodcraft, Camelia Dunare, Rashmikant V. Sudiwala, T. Peacocke, Michael D. Audley, Dan Bintley, T. Hodson, Kent D. Irwin, Matthew Joseph Griffin, Peter A. R. Ade, Wayne S. Holland, Fred Gannaway, D. Kelly, I. Smith, M. J. MacIntosh, W. D. Duncan, Pierre Bastien, Joel N. Ullom, David A. Naylor, David Atkinson, M. Fich, Giampaolo Pisano, Mark Halpern, M. Cliffe, George F. Mitchell, H. McGregor, Ian Walker, Anthony J. Walton, Ian Robson, Gene C. Hilton, and William Parkes
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Physics ,Nuclear and High Energy Physics ,business.industry ,Bolometer ,Detector ,Submillimeter detector array ,Large format ,Submillimetre astronomy ,Particle detector ,law.invention ,Optics ,law ,SQUID multiplexer ,Astronomical interferometer ,TES Bolometer array ,Transition edge sensor ,business ,Instrumentation ,Submillimeter detector array, Transition edge sensor, TES Bolometer array, SQUID multiplexer ,James Clerk Maxwell Telescope - Abstract
SCUBA-2, which replaces the Submillimetre Common User Bolometer Array (SCUBA) (Mon. Not. R. Astron. Soc. 303 (1999) 659) on the James Clerk Maxwell telescope in 2006, will be the first CCD-like array for submillimeter astronomy. Unlike previous detectors which have used discrete bolometers, SCUBA-2 has two DC-coupled, monolithic, filled arrays with a total of 10,000 bolometers. It will offer simultaneous imaging of an 8A—8 arcmin field of view at wavelengths of 850 and 450 I¼m. SCUBA-2 is expected to have a huge impact on the study of galaxy formation and evolution in the early Universe as well as star and planet formation in our own Galaxy. Mapping the sky to the same S/N up to 1000 times faster than SCUBA, it will also act as a pathfinder for the new submillimetre interferometers such as ALMA. SCUBA-2's absorber-coupled pixels use superconducting transition edge sensors (Ph.D. Thesis, Stanford, 1995) operating at 120 mK for photon noise limited performance. The monolithic silicon detector arrays are deep-etched by the Bosch process to isolate the pixels on silicon nitride membranes (Nucl. Instr. and Meth. A, these proceedings). Electrical connections are made through indium bump bonds to a backplane that incorporates a SQUID time-domain multiplexer. We describe the key technologies that make SCUBA-2 possible and give an update on the considerable progress in the detector development and instrument design that has taken place over the last 2 years
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- 2004
12. Spontaneous coronary artery dissection
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Patrick T. Koller and Charles M. Cliffe
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Adult ,Pregnancy ,Dissection ,Pregnancy Complications, Cardiovascular ,Myocardial Infarction ,Humans ,Radiology, Nuclear Medicine and imaging ,Coronary Disease ,Female ,General Medicine ,Puerperal Disorders ,Cardiology and Cardiovascular Medicine ,Coronary Angiography - Published
- 2001
13. Rice tungro disease is caused by an RNA and a DNA virus
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Roger Hull, R. Qu, B. L. Subba Rao, P. Shen, Maggi L. Blakebrough, J. M. Cliffe, K. Gough, M. Kaniewska, M. C. Jones, Jeffrey W. Davies, Roger N. Beachy, and Indranil Dasgupta
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Rice tungro bacilliform virus ,biology ,Genes, Viral ,Restriction Mapping ,DNA Viruses ,RNA ,RNA virus ,DNA virus ,Oryza ,biology.organism_classification ,Virology ,Molecular biology ,Virus ,Plant Viruses ,chemistry.chemical_compound ,Capsid ,chemistry ,Plant virus ,DNA, Viral ,Rice tungro spherical virus ,RNA Viruses ,RNA, Viral ,DNA ,Plant Diseases - Abstract
We present evidence that rice tungro spherical virus (RTSV) has a genome of polyadenylated single-stranded RNA of about 10 kb whereas rice tungro bacilliform virus (RTBV) contains double-stranded circular DNA. RTBV DNA has been mapped and shown to have two discontinuities, one in each strand, at specific sites; it thus resembles that of the caulimo-viruses. Gel electrophoresis of RTSV preparations revealed two protein bands (M r 35K and 26K). RTBV yielded two major protein bands of 37K and 33K together with several minor species of higher and lower M r which react with antiviral serum.
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- 1991
14. Book Review: The Certified Occupational Therapy Assistant. Principles, Concepts and Techniques
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Sylvia M Cliffe
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medicine.medical_specialty ,Medical education ,Occupational Therapy ,business.industry ,Alternative medicine ,medicine ,Certification ,Occupational Therapy Assistant ,business - Published
- 1994
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15. Book Review: Cota Examination Review Manual. A Practical Guide to Receiving Professional Certification
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Sylvia M Cliffe
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Professional certification (business) ,Medical education ,Occupational Therapy ,business.industry ,Medicine ,business - Published
- 1994
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16. Low dose cyclosporin a therapy in chronic posterior uveitis
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A M Cliffe, Hamish M.A. Towler, John V. Forrester, and Paul H. Whiting
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Adult ,Male ,medicine.medical_specialty ,Adolescent ,Side effect ,medicine.medical_treatment ,Visual Acuity ,Cyclosporins ,Gastroenterology ,Nephrotoxicity ,chemistry.chemical_compound ,Oral administration ,Internal medicine ,Cyclosporin a ,Humans ,Medicine ,Creatinine ,Chemotherapy ,Dose-Response Relationship, Drug ,business.industry ,Maintenance dose ,Uveitis, Posterior ,Middle Aged ,medicine.disease ,Surgery ,Ophthalmology ,chemistry ,Chronic Disease ,Female ,business ,Uveitis ,Follow-Up Studies - Abstract
Nine patients with chronic posterior endogenous uveitis of varying aetiology, not satisfactorily controlled with systemic corticosteroids alone, were treated with low dose oral Cyclosporin A (mean maintenance dose 4.0 +/- 1.1 mg/kg/day). Six of the nine patients also continued to receive oral prednisolone (15 mg/day or less). During a follow up period of 6-30 (mean 17) months, seven have shown sustained visual improvement. Nephrotoxicity was the major side effect with serum creatinine concentration (mean +/- 1SD) rising from 91 +/- 7 mumol/l to 115 +/- 13 mumol/l (0.01 less than p less than 0.05) after the first twelve months of treatment. Other observed side effects have included taste disturbance, hypomagnesaemia, and hypertension. Cyclosporin A treatment is of value in the management of severe intraocular inflammation, unresponsive to conventional therapy, but the optimum duration of therapy remains to be established and nephrotoxicity is the major dose limiting effect.
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- 1989
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17. Superoxide enhances photobleaching during cellular immune attack against fluorescent lipid monolayer membranes
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Dean G. Hafeman, Charles M. Cliffe, Harden M. McConnell, and Michael Seul
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Cellular immunity ,Neutrophils ,Photochemistry ,Guinea Pigs ,Biophysics ,Phospholipid ,Biochemistry ,Superoxide dismutase ,chemistry.chemical_compound ,Oxygen Consumption ,Superoxides ,Cell Adhesion ,Animals ,Humans ,Fluorescein ,biology ,Superoxide ,Macrophages ,Antibody-Dependent Cell Cytotoxicity ,Proteolytic enzymes ,Membranes, Artificial ,Cell Biology ,Fluoresceins ,Photobleaching ,Membrane ,Microscopy, Fluorescence ,chemistry ,Immunoglobulin G ,biology.protein ,Rabbits ,Lysosomes - Abstract
Lipid hapten-containing monolayer membranes with bound, anti-hapten antibody molecules serve as model immunological target membranes. Targets with bound-IgG trigger guinea pig macrophages to (a) adhere, (b) spread, (c) release lysosomal enzymes, and (d) increase cyanide-insensitive oxygen consumption. When the target membranes are derivatized with fluorescein, there is a 2-3-fold enhancement in the rate of fluorescein photobleaching in regions of cell-monolayer contact. This effect is due to release of O2- from macrophages, as shown by inhibition with superoxide dismutase and by the fact that enhanced photobleaching is not observed with cells of the RAW264 macrophage line, which undergo responses (a)-(d), but do not release O2- extracellularly. The O2- dependent photobleaching reaction appears to be relatively specific for fluorescein, as it did not occur with two other fluorophores, 4-nitrobenz-2-oxa-1,3-diazole and tetramethyl-rhodamine. Because stimulated neutrophils release large quantities of O2-, the photobleaching of fluorescein-labeled target membranes in response to neutrophils was examined. Monolayer membranes with specifically bound IgG caused neutrophils to adhere and become markedly motile during incubation at 37 degrees C. Like macrophages, neutrophils induced O2- -dependent photobleaching of fluorescein-labeled IgG in regions of cell-monolayer contact. In addition, neutrophils gave rise to a slower, nonphotochemical loss of fluorescence in the same contact regions. The latter effect is apparently due to cleavage of target-bound fluorescent IgG by proteolytic enzymes secreted by the neutrophils in response to the target surface.
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- 1984
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18. Energetic Electron Precipitation Driven by Electromagnetic Ion Cyclotron Waves from ELFIN's Low Altitude Perspective.
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Angelopoulos V, Zhang XJ, Artemyev AV, Mourenas D, Tsai E, Wilkins C, Runov A, Liu J, Turner DL, Li W, Khurana K, Wirz RE, Sergeev VA, Meng X, Wu J, Hartinger MD, Raita T, Shen Y, An X, Shi X, Bashir MF, Shen X, Gan L, Qin M, Capannolo L, Ma Q, Russell CL, Masongsong EV, Caron R, He I, Iglesias L, Jha S, King J, Kumar S, Le K, Mao J, McDermott A, Nguyen K, Norris A, Palla A, Roosnovo A, Tam J, Xie E, Yap RC, Ye S, Young C, Adair LA, Shaffer C, Chung M, Cruce P, Lawson M, Leneman D, Allen M, Anderson M, Arreola-Zamora M, Artinger J, Asher J, Branchevsky D, Cliffe M, Colton K, Costello C, Depe D, Domae BW, Eldin S, Fitzgibbon L, Flemming A, Frederick DM, Gilbert A, Hesford B, Krieger R, Lian K, McKinney E, Miller JP, Pedersen C, Qu Z, Rozario R, Rubly M, Seaton R, Subramanian A, Sundin SR, Tan A, Thomlinson D, Turner W, Wing G, Wong C, and Zarifian A
- Abstract
We review comprehensive observations of electromagnetic ion cyclotron (EMIC) wave-driven energetic electron precipitation using data collected by the energetic electron detector on the Electron Losses and Fields InvestigatioN (ELFIN) mission, two polar-orbiting low-altitude spinning CubeSats, measuring 50-5000 keV electrons with good pitch-angle and energy resolution. EMIC wave-driven precipitation exhibits a distinct signature in energy-spectrograms of the precipitating-to-trapped flux ratio: peaks at >0.5 MeV which are abrupt (bursty) (lasting ∼17 s, or Δ L ∼ 0.56 ) with significant substructure (occasionally down to sub-second timescale). We attribute the bursty nature of the precipitation to the spatial extent and structuredness of the wave field at the equator. Multiple ELFIN passes over the same MLT sector allow us to study the spatial and temporal evolution of the EMIC wave - electron interaction region. Case studies employing conjugate ground-based or equatorial observations of the EMIC waves reveal that the energy of moderate and strong precipitation at ELFIN approximately agrees with theoretical expectations for cyclotron resonant interactions in a cold plasma. Using multiple years of ELFIN data uniformly distributed in local time, we assemble a statistical database of ∼50 events of strong EMIC wave-driven precipitation. Most reside at L ∼ 5 - 7 at dusk, while a smaller subset exists at L ∼ 8 - 12 at post-midnight. The energies of the peak-precipitation ratio and of the half-peak precipitation ratio (our proxy for the minimum resonance energy) exhibit an L -shell dependence in good agreement with theoretical estimates based on prior statistical observations of EMIC wave power spectra. The precipitation ratio's spectral shape for the most intense events has an exponential falloff away from the peak (i.e., on either side of ∼ 1.45 MeV). It too agrees well with quasi-linear diffusion theory based on prior statistics of wave spectra. It should be noted though that this diffusive treatment likely includes effects from nonlinear resonant interactions (especially at high energies) and nonresonant effects from sharp wave packet edges (at low energies). Sub-MeV electron precipitation observed concurrently with strong EMIC wave-driven >1 MeV precipitation has a spectral shape that is consistent with efficient pitch-angle scattering down to ∼ 200-300 keV by much less intense higher frequency EMIC waves at dusk (where such waves are most frequent). At ∼100 keV, whistler-mode chorus may be implicated in concurrent precipitation. These results confirm the critical role of EMIC waves in driving relativistic electron losses. Nonlinear effects may abound and require further investigation., Competing Interests: Competing InterestsThe authors have no relevant financial or non-financial interests to disclose., (© The Author(s) 2023.)
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- 2023
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19. Can you see me? Participant experience of accessing a weight management programme via group videoconference to overcome barriers to engagement.
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Cliffe M, Di Battista E, and Bishop S
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- Adolescent, Adult, Female, Humans, Male, Middle Aged, Pandemics, Patient Satisfaction, SARS-CoV-2, Wales, Young Adult, COVID-19 epidemiology, Obesity therapy, Telemedicine organization & administration, Videoconferencing, Weight Reduction Programs organization & administration
- Abstract
Background: Engagement with conventional weight management group programmes is low., Objective: To understand participant experience of accessing an adapted programme via videoconference., Participants: Adults with obesity (BMI ≥ 35kg/m
2 ), referred to an NHS Dietetics service in Wales, were offered a group videoconference weight management programme as an optional alternative to in-person groups. Thirteen participants (mean age 48.5 ± 20.2 years, 8 female) recruited to two videoconference groups were interviewed., Study Design: A Registered Dietitian delivered a behavioural programme using Skype for Business in 10 sessions over 6 months. Participants joined the groups from any Internet-connected device with a webcam. Participant perspectives were audiorecorded in one-to-one, semi-structured interviews. Interviews were transcribed verbatim and thematically analysed using self-determination theory as a theoretical framework., Results: Ten themes were identified, three relating to service engagement and seven relating to behaviour change facilitation. Key themes in engagement included 'reduced burden', described as saving time and travel and 'reduced threat' as participants perceived joining a group from home as less daunting compared to attending in-person. Despite reporting some initial technical difficulties with establishing video and audio connection, participants described beneficial peer support although not physically with other group members., Conclusion: Accessing a group weight management programme via videoconference may be the preferred option for some participants, overcoming some of the barriers to access to standard in-person programmes, particularly in rural areas. Participants are able to experience peer support via videoconference. During the COVID-19 pandemic, weight management programmes could utilize videoconference groups to continue to provide support., (© 2020 The Authors. Health Expectations published by John Wiley & Sons Ltd.)- Published
- 2021
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20. The ELFIN Mission.
- Author
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Angelopoulos V, Tsai E, Bingley L, Shaffer C, Turner DL, Runov A, Li W, Liu J, Artemyev AV, Zhang XJ, Strangeway RJ, Wirz RE, Shprits YY, Sergeev VA, Caron RP, Chung M, Cruce P, Greer W, Grimes E, Hector K, Lawson MJ, Leneman D, Masongsong EV, Russell CL, Wilkins C, Hinkley D, Blake JB, Adair N, Allen M, Anderson M, Arreola-Zamora M, Artinger J, Asher J, Branchevsky D, Capitelli MR, Castro R, Chao G, Chung N, Cliffe M, Colton K, Costello C, Depe D, Domae BW, Eldin S, Fitzgibbon L, Flemming A, Fox I, Frederick DM, Gilbert A, Gildemeister A, Gonzalez A, Hesford B, Jha S, Kang N, King J, Krieger R, Lian K, Mao J, McKinney E, Miller JP, Norris A, Nuesca M, Palla A, Park ESY, Pedersen CE, Qu Z, Rozario R, Rye E, Seaton R, Subramanian A, Sundin SR, Tan A, Turner W, Villegas AJ, Wasden M, Wing G, Wong C, Xie E, Yamamoto S, Yap R, Zarifian A, and Zhang GY
- Abstract
The Electron Loss and Fields Investigation with a Spatio-Temporal Ambiguity-Resolving option (ELFIN-STAR, or heretoforth simply: ELFIN) mission comprises two identical 3-Unit (3U) CubeSats on a polar (∼93
∘ inclination), nearly circular, low-Earth (∼450 km altitude) orbit. Launched on September 15, 2018, ELFIN is expected to have a >2.5 year lifetime. Its primary science objective is to resolve the mechanism of storm-time relativistic electron precipitation, for which electromagnetic ion cyclotron (EMIC) waves are a prime candidate. From its ionospheric vantage point, ELFIN uses its unique pitch-angle-resolving capability to determine whether measured relativistic electron pitch-angle and energy spectra within the loss cone bear the characteristic signatures of scattering by EMIC waves or whether such scattering may be due to other processes. Pairing identical ELFIN satellites with slowly-variable along-track separation allows disambiguation of spatial and temporal evolution of the precipitation over minutes-to-tens-of-minutes timescales, faster than the orbit period of a single low-altitude satellite (Torbit ∼ 90 min). Each satellite carries an energetic particle detector for electrons (EPDE) that measures 50 keV to 5 MeV electrons with Δ E/E < 40% and a fluxgate magnetometer (FGM) on a ∼72 cm boom that measures magnetic field waves (e.g., EMIC waves) in the range from DC to 5 Hz Nyquist (nominally) with <0.3 nT/sqrt(Hz) noise at 1 Hz. The spinning satellites (Tspin ∼ 3 s) are equipped with magnetorquers (air coils) that permit spin-up or -down and reorientation maneuvers. Using those, the spin axis is placed normal to the orbit plane (nominally), allowing full pitch-angle resolution twice per spin. An energetic particle detector for ions (EPDI) measures 250 keV - 5 MeV ions, addressing secondary science. Funded initially by CalSpace and the University Nanosat Program, ELFIN was selected for flight with joint support from NSF and NASA between 2014 and 2018 and launched by the ELaNa XVIII program on a Delta II rocket (with IceSatII as the primary). Mission operations are currently funded by NASA. Working under experienced UCLA mentors, with advice from The Aerospace Corporation and NASA personnel, more than 250 undergraduates have matured the ELFIN implementation strategy; developed the instruments, satellite, and ground systems and operate the two satellites. ELFIN's already high potential for cutting-edge science return is compounded by concurrent equatorial Heliophysics missions (THEMIS, Arase, Van Allen Probes, MMS) and ground stations. ELFIN's integrated data analysis approach, rapid dissemination strategies via the SPace Environment Data Analysis System (SPEDAS), and data coordination with the Heliophysics/Geospace System Observatory (H/GSO) optimize science yield, enabling the widest community benefits. Several storm-time events have already been captured and are presented herein to demonstrate ELFIN's data analysis methods and potential. These form the basis of on-going studies to resolve the primary mission science objective. Broad energy precipitation events, precipitation bands, and microbursts, clearly seen both at dawn and dusk, extend from tens of keV to >1 MeV. This broad energy range of precipitation indicates that multiple waves are providing scattering concurrently. Many observed events show significant backscattered fluxes, which in the past were hard to resolve by equatorial spacecraft or non-pitch-angle-resolving ionospheric missions. These observations suggest that the ionosphere plays a significant role in modifying magnetospheric electron fluxes and wave-particle interactions. Routine data captures starting in February 2020 and lasting for at least another year, approximately the remainder of the mission lifetime, are expected to provide a very rich dataset to address questions even beyond the primary mission science objective., (© The Author(s) 2020.)- Published
- 2020
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21. Ecological momentary assessment of food perceptions and eating behavior using a novel phone application in adults with or without obesity.
- Author
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Alabduljader K, Cliffe M, Sartor F, Papini G, Cox WM, and Kubis HP
- Subjects
- Adolescent, Adult, Aged, Craving, Ecological Momentary Assessment, Female, Humans, Male, Middle Aged, Mobile Applications, Reward, Smartphone, Young Adult, Feeding Behavior, Food, Obesity epidemiology, Perception
- Abstract
We developed a smart phone application to measure participants' food-reward perceptions and eating behavior in their naturalistic environment. Intensity ratings (0 - not at all to 10 - very strongly) of perceived anticipation of food (wanting) and food enjoyment at endpoint of intake (liking) were recorded as they occurred over a period of 14 days. Moreover, food craving trait, implicit and explicit attitude towards healthy food, and body composition were assessed. 53 participants provided complete data. Participants were classified by percentage of body fat; 33 participants with lower body fat (L-group) and 20 with higher body fat (H-group; ≥25% body fat for males and ≥32% for females). L-group participants reported 6.34 (2.00) food wanting events per day, whereas H-group participants recorded significantly fewer food wanting events (5.07 (1.42)); both groups resisted about the same percentage of wanting events (L-group: 29.2 (15.5)%; H-group 27.3 (12.8)%). Perceived intensity ratings were significantly different within the L-group in the order liking (7.65 (0.81)) > un-resisted wanting (leading to eating) (7.00 (1.01)) > resisted wanting (not leading to eating) (6.02 (1.72)) but not in the H-group. Liking scores (L-group: 7.65 (0.81); H-group: 7.14 (1.04)) were significantly higher in L-group than in H-group after controlling for age. Our results show that individuals with higher percentage of body fat show less food enjoyment after intake and reveal no differentiation in intensity ratings of perceived anticipatory and consummatory food reward. These results are consistent with a hypothesized reward deficiency among individuals with higher percentage of body fat., (Crown Copyright © 2018. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2018
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22. Situational analysis of lymphatic filariasis morbidity in Ahanta West District of Ghana.
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Stanton MC, Best A, Cliffe M, Kelly-Hope L, Biritwum NK, Batsa L, and Debrah A
- Subjects
- Costs and Cost Analysis, Elephantiasis, Filarial complications, Female, Focus Groups, Ghana, Health Expenditures, Health Personnel, Humans, Lymphedema etiology, Lymphedema therapy, Male, Morbidity, Testicular Hydrocele etiology, Testicular Hydrocele therapy, Disease Management, Elephantiasis, Filarial therapy, Health Services, Health Services Accessibility, Patient Acceptance of Health Care, Self Care
- Abstract
Objectives: Situational analysis of lymphatic filariasis (LF) morbidity and its management in Ahanta West, Ghana, to identify potential barrier to healthcare for LF patients., Methods: Lymphoedema and hydrocoele patients were identified by community health workers from a subset of villages, and were interviewed and participated in focus group discussions to determine their attitudes and practices towards managing their morbidity, and their perceived barriers to accessing care. Local health professionals were also interviewed to obtain their views on the availability of morbidity management services in the district., Results: Sixty-two patients (34 lymphoedema and 28 hydrocoeles) and 13 local health professionals were included in the study. Lymphoedema patients predominantly self-managed their conditions, which included washing with soap and water (61.8%), and exercising the affected area (52.9%). Almost 65% of patients had sought medical assistance at some stage, but support was generally limited to receiving tablets (91%). Local health professionals reported rarely seeing lymphoedema patients, citing stigma and lack of provisions to assist patients as a reason for this. Almost half of hydrocoele patients (44%) chose not to seek medical assistance despite the negative impact it had on their lives. Whilst surgery itself is free with national health insurance, 63% those who had not sought treatment stated that indirect costs of surgery (travel costs, loss of earnings, etc.) were the most prohibitive factor to seeking treatment., Conclusions: The information obtained from this study should now be used to guide future morbidity strategies in building a stronger relationship between the local health services and LF patients, to ultimately improve patients' physical, psychological and economic wellbeing., (© 2015 The Authors. Tropical Medicine & International Health Published by John Wiley & Sons Ltd.)
- Published
- 2016
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23. Developing a community-led SMS reporting tool for the rapid assessment of lymphatic filariasis morbidity burden: case studies from Malawi and Ghana.
- Author
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Stanton MC, Mkwanda SZ, Debrah AY, Batsa L, Biritwum NK, Hoerauf A, Cliffe M, Best A, Molineux A, and Kelly-Hope LA
- Subjects
- Adolescent, Adult, Aged, Community Health Workers psychology, Elephantiasis, Filarial pathology, Female, Ghana epidemiology, Humans, Malawi epidemiology, Male, Middle Aged, Morbidity, Prevalence, Severity of Illness Index, Software, Surveys and Questionnaires, Young Adult, Elephantiasis, Filarial epidemiology, Telemedicine
- Abstract
Background: Lymphoedema and hydrocoele are the two most common clinical manifestations of lymphatic filariasis (LF). In order to effectively target morbidity management strategies, more information is rapidly needed on morbidity burden across all endemic countries. The purpose of this study was to develop and test an SMS tool (MeasureSMS) which enables trained community-based health workers to report basic information on all cases they identified., Methods: The tool was trialled in Chikwawa district, Malawi and Ahanta West district, Ghana in 2014. Salaried health surveillance assistants (HSAs) identified and reported cases in Malawi whereas volunteer community health workers (CHWs) were used in Ghana. Health workers were trained in recognising lymphoedema and hydrocoeles and submitting individual case data using MeasureSMS, after which they undertook a LF morbidity survey. After the reporting period, a random sample of reported cases was visited by a physician to verify the health workers' diagnoses. The proportion of correctly diagnosed cases i.e. the positive predictive value (PPV) was then calculated., Results: HSAs in Malawi successfully reported 256 unique cases by SMS from 107 communities (166 hydrocoele, 88 lymphoedema, 2 with both), resulting in an estimated adult prevalence of 17.7 per 10,000 and 33.0 per 10,000 for lymphoedema and hydrocoele respectively. In Ghana, despite being less experienced in using SMS, CHWs successfully reported 360 unique cases by SMS from 33 communities (169 hydrocoele, 185 lymphoedema, 6 with both), resulting in an estimated adult prevalence of 76.9 per 10,000 and 70.5 per 10,000 adults for lymphoedema and hydrocoele respectively. The verification exercise resulted in a PPV for lymphoedema and hydrocoele diagnosis of 90 % (n = 42, 95 % CI 76.5 - 96.9) and 92 % (n = 49, 95 % CI 79.5 - 97.4) in Malawi and 94 % (n = 34, 95 % CI 78.9 %-99.0 %) and 47 % (n = 59, 35.1 %-61.7 %) in Ghana, indicating that non-invasive methods for diagnosing hydrocoeles needed to be further emphasised., Conclusions: The study concludes that given the appropriate education and tools, community-based health workers are exceptionally well-placed to participate in quantifying LF morbidity burden, and other NTDs with observable symptoms. This concept has the potential to enable national programmes to more effectively monitor their community impact in an efficient, timely and cost-effective way.
- Published
- 2015
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24. Linking environmental assessment to environmental regulation through adaptive management.
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Racher KI, Hutchinson N, Hart D, Fraser B, Clark B, Fequet R, Ewaschuk P, and Cliffe-Phillips M
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- Environmental Monitoring methods, Risk Assessment methods
- Published
- 2011
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25. Can malnutrition in predialysis patients be prevented by dietetic intervention?
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Cliffe M, Bloodworth LL, and Jibani MM
- Subjects
- Anthropometry, Counseling, Dietary Proteins administration & dosage, Female, Glomerular Filtration Rate, Humans, Longitudinal Studies, Male, Middle Aged, Prospective Studies, Diet, Energy Intake, Kidney Diseases complications, Nutrition Disorders prevention & control, Nutritional Status
- Abstract
Objective: This study aimed to discover if the documented decline in nutritional status in predialysis patients could be prevented by dietetic intervention., Design: Longitudinal prospective interventional study., Setting: General hospital nephrology clinic., Patients: Eleven patients with progressive chronic renal failure not yet requiring dialysis, all with creatinine clearance below 25 mL/min were studied. Mean age was 63.9 +/- 14.5 years., Intervention: Patients received nutritional counseling from a renal dietitian on at least 3 occasions over a period of 6 months. Following assessment, patients were advised on dietary changes according to individual need, aiming for adequate energy intake to achieve or maintain a body mass index of 20 to 25 and protein intake of 0.8 to 1.0 g/kg/d. Dietary supplements were prescribed when necessary., Outcome Measures: Changes in nutritional status were assessed by Subjective Global Assessment, anthropometric measures (weight, triceps skinfold thickness, mid arm muscle circumference, and grip strength), and biochemical markers (serum albumin, serum transferrin, and insulin-like growth factor-1)., Results: None of the patients showed decline in Subjective Global Assessment category, and 2 of the patients improved. All anthropometric and biochemical measures of nutritional status were stable or increased over the course of the study, and mid arm muscle circumference increased significantly (P <.05), contrasting with published data showing a decline in these measures in patients not receiving dietetic intervention., Conclusion: With dietetic intervention, it may be possible to maintain or improve nutritional status in this group., (Copyright 2001 by the National Kidney Foundation, Inc.)
- Published
- 2001
- Full Text
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26. Modified Personal Questionnaire Rapid Scaling Technique for measuring delusional beliefs.
- Author
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Cliffe M, Possamai A, and Mulhall D
- Subjects
- Humans, Delusions diagnosis, Surveys and Questionnaires
- Abstract
The view of delusions as discrete and discontinuous phenomena is changing. Intensity of conviction in delusional belief has been used as a dependent variable in therapeutic attempts at belief modification. It has been measured using rating scales and developments of Shapiro's Personal Questionnaire (PQ) technique. The use of Mulhall's Personal Questionnaire Rapid Scaling Technique (PQRST) has not been reported in this context. The paper reports no new clinical data but describes a modified PQRST for measuring intensity of belief conviction, suitable for clients who cannot understand the PQ technique and easily administered by ward staff.
- Published
- 1995
- Full Text
- View/download PDF
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