82 results on '"M. Basti"'
Search Results
2. A Rare Case of a Pregnant COVID-19 Patient That Survived an Emergency Bedside Caesarean Section and ECMO Therapy
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A. Farooqui, S.M.M. Uddin, S. Maheshwari, S. Fan, G. Randhawa, C. Seneviratne, and M. Basti
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- 2022
3. On the short-term response of entrained air bubbles in the upper ocean: a case study in the north Adriatic Sea
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A. Benetazzo, T. Halsne, Ø. Breivik, K. O. Strand, A. H. Callaghan, F. Barbariol, S. Davison, F. Bergamasco, C. Molina, and M. Bastianini
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Geography. Anthropology. Recreation ,Environmental sciences ,GE1-350 - Abstract
Air bubbles in the upper ocean are generated mainly by waves breaking at the air–sea interface. As such, after the waves break, entrained air bubbles evolve in the form of plumes in the turbulent flow, exchange gas with the surrounding water, and may eventually rise to the surface. To shed light on the short-term response of entrained bubbles in different stormy conditions and to assess the link between bubble plume penetration depth, mechanical and thermal forcings, and the air–sea transfer velocity of CO2, a field experiment was conducted from an oceanographic research tower in the north Adriatic Sea. Air bubble plumes were observed using high-resolution echosounder data from an upward-looking 1000 kHz sonar. The backscatter signal strength was sampled at a high resolution, 0.5 s in time and 2.5 cm along the vertical direction. Time series profiles of the bubble plume depth were established using a variable threshold procedure applied to the backscatter strength. The data show the occurrence of bubbles organized into vertical plume-like structures, drawn downwards by wave-generated turbulence and other near-surface circulations, and reaching the seabed at 17 m depth under strong forcing. We verify that bubble plumes adapt rapidly to wind and wave conditions and have depths that scale approximately linearly with wind speed. Scaling with the wind–wave Reynolds number is also proposed to account for the sea-state severity in the plume depth prediction. Results show a correlation between measured bubble depths and theoretical air–sea CO2 transfer velocity parametrized with wind-only and wind/wave formulations. Further, our measurements corroborate previous results suggesting that the sinking of newly formed cold-water masses helps bring bubbles to greater depths than those reached in stable conditions for the water column. The temperature difference between air and sea seems sufficient for describing this intensification at the leading order of magnitude. The results presented in this study are relevant for air–sea interaction studies and pave the way for progress in CO2 gas exchange formulations.
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- 2024
- Full Text
- View/download PDF
4. Correction to: Laparoscopic right hemicolectomy: the SICE (Società Italiana di Chirurgia Endoscopica e Nuove Tecnologie) network prospectivetrial on 1225 cases comparing intra corporeal versus extra corporeal ileo‑colic side‑to‑side anastomosis (Surgical Endoscopy, (2019), 10.1007/s00464-019-07255-2)
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M. Sorrentino, A. Alo, G. L. Canu, F. Monari, A. G. Marrosu, E. Soligo, Wanda Petz, A. Gattolin, R. Vicentini, S. Razzi, M. Zago, S. Neri, A. Pisani Ceretti, D. Apa, F. Gatti, A. Donini, F. Medas, D. Cassetti, S. Rubino, R. Lombardi, G. D. DePalma, Alberto Arezzo, G. Soliani, P. Checcacci, G. Concone, Emanuele Botteri, F. Scognamillo, Ferdinando Agresta, Pierluigi Marini, S. Gelati, Luigi Boni, A. Coratti, Andrea Picchetto, G. Guerriero, M. Calgaro, Francesca Pecchini, A. Contine, Andrea Valeri, N. DeManzini, M. Clementi, A. Balani, F. Fidanza, R. Galleano, Carlo Bergamini, A. Brescia, G. Arcuri, Elio Jovine, E. Rosso, A. Oldani, E. Artioli, Nereo Vettoretto, Giuseppe Navarra, G. Sarro, E. Restini, Chiara Morotti, S. Giannessi, F. DeAngelis, M. Degiuli, G. Talamo, G. Alemanno, L. Cafagna, P. Cumbo, V. Violi, S. Targa, Irnerio Angelo Muttillo, A. Martino, M. DeLuca, Elisa Cassinotti, Alessandro Puzziello, S. Sala, Riccardo Rosati, E. Erdas, R. Petri, A. Deserra, A. Gioffre, G. Viola, E. Stratta, Mario Guerrieri, E. Minciotti, Mauro Podda, Giuseppe Spinoglio, F. Borghi, Micaela Piccoli, C. DeNisco, P. Carcoforo, D. Delogu, Giuseppe Resta, P. Corleone, D. Pennisi, Gianfranco Silecchia, E. Opocher, A. Taddei, A. Budassi, Paolo Delrio, A. Meloni, Marco E. Allaix, A. Ambrosi, H. Impellizzeri, N. Portolani, L. Guerriero, G. Ercolani, A. Guariniello, M. Antoniutti, M. Cesari, A. P. Luzzi, M. Izzo, M. Longoni, R. Mazza, C. Benvenuto, S. Gobbi, P. G. Calo, C. Feo, Antonino Agrusa, L. Covotta, L. Presenti, V. Adamo, Gian Luca Baiocchi, E. Osenda, R. Ottonello, Giancarlo D'Ambrosio, F. Roviello, V. Grammatico, G. Moretto, L. Zampino, Valerio Caracino, Giovanni Ferrari, D. Rega, V. Robustelli, Diego Cuccurullo, F. Vasta, Ugo Elmore, R. Campagnacci, Gianfranco Cocorullo, O. Ghazouani, G. Ricci, S. Berti, F. Colombo, Alberto Sartori, S. Scabini, S. Mazzoccato, B. Pirrera, A. Altamura, N. Tartaglia, E. Romairone, G. Baldazzi, Marco Catarci, G. Garulli, Lorenzo Casali, S. Testa, R. Brachet Contul, M. Basti, U. Rivolta, D. Pertile, M. Pavanello, M. Pisano, Marco Milone, A. Verzelli, P. Ubiali, L. Solaini, M. Coppola, G Anania, Massimo Carlini, F. Corcione, P. DePaolis, P. Ciano, M. Santarelli, V. Panebianco, Nicola Perrotta, R. Sechi, M. Rigamonti, G. Lezoche, L. Fabris, C. Lirusso, D. Foschi, G. Canova, P. Soliani, Roberta Gelmini, Stefano Olmi, A. Lucchi, Giorgia Valpiani, L. Pellegrino, Anania, G., Agresta, F., Artioli, E., Rubino, S., Resta, G., Vettoretto, N., Petz, W. L., Bergamini, C., Arezzo, A., Valpiani, G., Morotti, C., Silecchia, G., Adamo, V., Agrusa, A., Alemanno, G., Allaix, M. E., Alo, A., Altamura, A., Ambrosi, A., Antoniutti, M., Apa, D., Arcuri, G., Baiocchi, G. L., Balani, A., Baldazzi, G., Basti, M., Benvenuto, C., Berti, S., Boni, L., Borghi, F., Botteri, E., Brachet Contul, R., Brescia, A., Budassi, A., Cafagna, L., Calgaro, M., Calo, P. G., Campagnacci, R., Canova, G., Canu, G. L., Caracino, V., Carcoforo, P., Carlini, M., Casali, L., Cassetti, D., Cassinotti, E., Catarci, M., Cesari, M., Checcacci, P., Ciano, P., Clementi, M., Cocorullo, G., Colombo, F., Concone, G., Contine, A., Coppola, M., Coratti, A., Corcione, F., Corleone, P., Covotta, L., Cuccurullo, D., Cumbo, P., D'Ambrosio, G., Deangelis, F., Deluca, M., Demanzini, N., Denisco, C., Depalma, G. D., Depaolis, P., Degiuli, M., Delogu, D., Delrio, P., Deserra, A., Donini, A., Elmore, U., Ercolani, G., Erdas, E., Fabris, L., Ferrari, G., Feo, C., Fidanza, F., Foschi, D., Galleano, R., Garulli, G., Gatti, F., Gattolin, A., Gelati, S., Gelmini, R., Ghazouani, O., Gioffre, A., Gobbi, S., Grammatico, V., Guariniello, A., Giannessi, S., Guerrieri, M., Guerriero, L., Guerriero, G., Impellizzeri, H., Izzo, M., Jovine, E., Lezoche, G., Lirusso, C., Lombardi, R., Longoni, M., Lucchi, A., Luzzi, A. P., Marini, P., Marrosu, A. G., Martino, A., Mazza, R., Mazzoccato, S., Medas, F., Meloni, A., Milone, M., Minciotti, E., Monari, F., Moretto, G., Muttillo, I. A., Navarra, G., Neri, S., Oldani, A., Olmi, S., Opocher, E., Osenda, E., Ottonello, R., Panebianco, V., Pavanello, M., Pecchini, F., Pellegrino, L., Pennisi, D., Perrotta, N., Pertile, D., Petri, R., Picchetto, A., Piccoli, M., Pirrera, B., Pisani Ceretti, A., Pisano, M., Podda, M., Portolani, N., Presenti, L., Puzziello, A., Razzi, S., Rega, D., Restini, E., Ricci, G., Rigamonti, M., Rivolta, U., Robustelli, V., Romairone, E., Rosati, R., Rosso, E., Roviello, F., Sala, S., Santarelli, M., Sarro, G., Sartori, A., Scabini, S., Scognamillo, F., Sechi, R., Solaini, L., Soliani, G., Soliani, P., Soligo, E., Sorrentino, M., Spinoglio, G., Stratta, E., Taddei, A., Talamo, G., Targa, S., Tartaglia, N., Testa, S., Ubiali, P., Valeri, A., Vasta, F., Verzelli, A., Vicentini, R., Viola, G., Violi, V., Zago, M., and Zampino, L.
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medicine.medical_specialty ,colon cancer right hemcolectomy ,business.industry ,medicine ,Surgery ,business ,Side to side anastomosis ,Surgical endoscopy ,Laparoscopic right hemicolectomy ,NO ,LS7_4 - Abstract
Due to an error in production the members of SICE CoDIG (Colon Dx Italian Group) listed in the Acknowledgments were not tagged correctly as authors in the XML of this article. This listing is presented again here:.
- Published
- 2019
5. Investigation of the degree of cross-linking of polyethylene and thermosets using absolute optical spectroscopy and Raman microscopy
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F. Bergmann, N. Halmen, C. Scalfi-Happ, D. Reitzle, A. Kienle, L. Mittelberg, B. Baudrit, T. Hochrein, and M. Bastian
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Technology - Abstract
In the research work presented here, an integrating sphere demonstrator which is suitable for the non-destructive determination of the degree of cross-linking or curing and has the potential for use as an at-line device for in-process quality assurance was assembled and explored. The measurement system allows the analysis of absorption and scattering coefficients of materials independently by means of absolute optical spectroscopy. The two optical parameters showed a good correlation with the degree of cross-linking of cross-linked polyethylene (PE-X) and the degree of curing of different thermosets and adhesives, each of which was determined using different reference methods (wet chemical analysis, differential scanning calorimetry (DSC), and dielectric analysis (DEA)). The results show that different PE-X materials can be distinguished well by their absorption and scattering in the visual (VIS) and near-infrared (NIR) wavelength range, respectively, and conclusions on their degree of cross-linking are possible. Also, the curing of resins can be monitored based on the absorption. In addition, Raman spectroscopy was used to achieve a better understanding of the material changes during the cross-linking of the materials. It also showed a good suitability for monitoring the curing processes in thermosets. In summary, the new method can be used to determine the crucial parameters of these industrial important material types and fulfils the great demand for fast, non-destructive testing, which can be carried out during the process or on the finished product.
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- 2023
- Full Text
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6. Central parks as air quality oases in the tropical Andean city of Quito
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R. Zalakeviciute, S. Bonilla Bedoya, D. Mejia Coronel, M. Bastidas, A. Buenano, and A. Diaz-Marquez
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Air pollution ,PM2.5 ,Urban parks ,City planning ,Terrain ,Environmental pollution ,TD172-193.5 ,Meteorology. Climatology ,QC851-999 - Abstract
Urban ecosystem is an intricate agglomeration of human, fauna and flora populations coexisting in natural and artificial environments. As a city develops and expands over time; it may become unbalanced, affecting the quality of ecosystem and urban services and leading to environmental and health problems. Fine particulate matter (particulate matter with aerodynamic diameter ≤2.5 μm - PM2.5) is the air pollutant posing the greatest risk to human health. Quito, the capital city of Ecuador, exhibits a high occurrence of exposure to unhealthy levels of PM2.5 due to a combination of natural and social variables. This study focused on three central parks of this high elevation city, investigating the spatial distribution of PM2.5 concentrations. The particle pollution was then modeled using Normalized Difference Vegetation Index (NDVI). Hazardous instantaneous levels of PM2.5 were consistently found on the edges of the parks along busy avenues, which are also the most frequented areas. This raises concerns about both short- and long-term exposures to toxic traffic pollution in recreational areas within urban dwellings in the global south. The NDVI model successfully predicted the spatial concentrations of PM2.5 in a smaller urban park, suggesting its potential application in other cities. However, further research is required to validate its effectiveness.
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- 2024
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7. Infiltration of Nanoparticles into Porous Binder Jet Printed Parts
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Mufeed M. Basti, Sarah AlSalihi, Amy M. Elliott, and Abbey Merriman
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0209 industrial biotechnology ,Environmental Engineering ,Aqueous solution ,Materials science ,General Computer Science ,General Chemical Engineering ,General Engineering ,Diethylene glycol ,Energy Engineering and Power Technology ,Nanoparticle ,02 engineering and technology ,021001 nanoscience & nanotechnology ,Geotechnical Engineering and Engineering Geology ,chemistry.chemical_compound ,020901 industrial engineering & automation ,chemistry ,Sodium hydroxide ,Composite material ,0210 nano-technology ,Suspension (vehicle) ,Porosity ,Mass fraction ,Ethylene glycol - Abstract
The densification of parts that are produced by binder jetting Additive Manufacturing (AM; a.k.a. “3D Printing”) is an essential step in making them mechanically useful. By increasing the packing factor of the powder bed by incorporating nanoparticles into the binder has potential to alleviate the amount of shrinkage needed for full densification of binder jet parts. We present preliminary data on the use of 316L Stainless Steel Nanoparticles (SSN) to densify 316L stainless steel binder jet parts. Aqueous solutions of Diethylene Glycol (DEG) or Ethylene Glycol (EG) were prepared at different DEG/water and EG/water molar ratios; pH of the solutions was adjusted by the use of 0.10 M sodium hydroxide. Nanoparticles were suspended in a resulted solution at a volume percentage of SSN/solution at 0.5%. The suspension was then sonicated for thirty minutes. One milliliter of the suspension was added stepwise to a sintered, printed disk with the dimensions: (d = 10 mm, h = 3 mm) in the presence of a small magnet. The 3D part was then sintered again. Moreover, the increase in the mass of the 3D part was used as indication of the amount of nanoparticles that diffused in the 3D part. This mass percent increasemore » was studied as a function of pH of the suspension and as function DEG/water molar ratio. Unlike EG, data show that change in pH affects the mass percent when the suspension was made with DEG. Finally, optical analysis of the discs’ cross sections revealed trends metallic densities similar to trends in the data for mass increase with changing pH and water molar ratio.« less
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- 2016
8. Physiochemical characterization of synthetic bio-oils produced from bio-mass: a sustainable source for construction bio-adhesives
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Shahrzad Hosseinnezhad, Mufeed M. Basti, Bidhya Kunwar, Brajendra K. Sharma, and Ellie H. Fini
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biology ,Chemistry ,General Chemical Engineering ,Biomass ,General Chemistry ,Miscanthus ,Raw material ,biology.organism_classification ,Pulp and paper industry ,Manure ,Hydrothermal liquefaction ,Corn stover ,Organic chemistry ,Pyrolysis ,Asphaltene - Abstract
This paper investigates physicochemical properties of four different types of bio-oil produced through hydrothermal liquefaction (HTL) and vacuum pyrolysis including wood pallet, corn stover, miscanthus and swine manure. It should be noted that the term bio-oil in this paper is used to refer to synthesized oil from post processing of biomass. Accordingly, swine manure was processed under HTL conditions of 340 °C, 10–12 MPa with 15 min residence time. Bio-oils from miscanthus, corn stover and wood pellet were produced at 450 °C under vacuum pyrolysis. Furthermore, in this paper the merit of applying each of these bio-oils as a precursor for producing bio-adhesive was studied using physiochemical and rheological characterization. Chemical functional groups and individual compounds were identified with GC-MS, NMR and FT-IR, while molecular weight distribution determined using GPC showed that wood pellet bio-oil has the lowest molecular weight followed by those from corn stover, miscanthus and swine manure. In addition, boiling point distributions of different fractions of bio-oils were analyzed. Furthermore, TLC-FID was used to determine different fractions of bio-oils based on their solubility in comparison with those of petroleum. It was shown that overall bio-oils from woody bio-mass have higher amount of alcoholic compounds as evidenced by the presence of strong peaks related to ether and alcohols in FTIR spectra; in addition, the TLC-FID analysis showed presence of higher fraction of fused poly aromatic rings referred to “asphaltene” in bio-oils produced from woody biomass compared to bio-oils from swine manure. The results of our characterization show the importance of feedstock composition and their effect on the characteristics of bio-oils as well as their applicability for use in bio-adhesives production.
- Published
- 2015
9. Influence of the type of phosphate additive and its concentration on the setting kinetics of CA cement bonded refractory castables with special regard to the resulting pH value
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J. Kasper, M. Bastian, and C. Dannert
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CA cement ,Hydration of CA cement ,Dormant period ,Sodium phosphate ,pH value ,Refractory castable ,Clay industries. Ceramics. Glass ,TP785-869 - Abstract
During the hydration of CA cement exists a dormant period, which is responsible for the setting kinetics of CA cement bonded refractory castables. The dormant period is believed to be caused by an Al–O–H passivation layer on the CA cement particles. Recent publications indicate that the dissolution (aging) of the passivation layer is closely connected to the prevailing pH value of the pore solution during setting. The higher the pH value, the faster the dissolution of the passivation layer.As phosphate additives, frequently used as dispersing agent, differ in their pH value when dissolved in water it is reasonable that they change the setting kinetics of CA cement bonded refractory castables. Commonly used sodium polyphosphate (S-PP, special case S-TPP for sodium tripolyphosphate) dispersing agents mainly differ in chain length, and therefore in Na+ and phosphate concentration, with different setting kinetics as a result.To investigate the influence of S-(T)PP on setting kinetics, sonic velocity and pH measurements were conducted on S-(T)PP/citric acid dispersed and CA cement bonded refractory castables during their setting. Different phosphates, differing in portion of Na+ to phosphate and medium chain length of the phosphate molecules, were used in varied concentrations within refractory castables to quantify the shift in setting kinetics and verify the role of the pH value during the hydration of CA cement.It was found that the chain length of the phosphate molecule of S-(T)PP influences the setting kinetics, and especially the length of the dormant period of the hydration of CA cement, in an indistinct way. Minor differences within the Na+ concentration at differing chain length S-(T)PP showed a comparable tendency to influence the hydration kinetics of CA cement as the chain length of the phosphate molecule was varied. However, an increase of the concentration of the applied S-(T)PP, which goes along with a distinctly increased Na+ concentration, resulted in a significant shorter dormant period and correspondingly an accelerated main hydration of CA cement. The corresponding pH value during the dormant period of the hydration of CA cement increased concurrently to the increase of the S-(T)PP and Na+ concentration.It is discussed that this increase of the pH value of the pore solution of the refractory castable results from an increase of the Na+ concentration from the S-(T)PP, while phosphate molecules are precipitated as Ca-phosphate. This indicates that the dissolution of the Al–O–H passivation layer accelerates with an increasing Na+ concentration (equivalent to increasing pH value) in the pore solution. Thereby, the dormant period is shorter and the hydration of CA cement starts earlier.The general tendency that additives of alkaline nature are accelerating the setting kinetics could be linked to the pH value during the dormant period. Therefore, the theory of the pH dependent aging of the Al–O–H passivation layer on CA cement particles was confirmed. As a directly applicable result, S-(T)PP may be used as accelerator (accelerating the CA cement hydration) beside their dispersing effect.
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- 2023
- Full Text
- View/download PDF
10. P.02.19 FUSOBACTERIUM NUCLEATUM (FN) IN PATIENTS AFFECTED BY INFLAMMATORY BOWEL DISEASE (IBD)
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Leonardo Marzio, M. Di Giulio, Antonio Francesco Ciccaglione, Luigina Cellini, M. Basti, A. D'Amico, and Laurino Grossi
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medicine.medical_specialty ,Hepatology ,biology ,business.industry ,Gastroenterology ,biology.organism_classification ,medicine.disease ,Inflammatory bowel disease ,Internal medicine ,medicine ,In patient ,Fusobacterium nucleatum ,business - Published
- 2018
11. Structural and functional roles of the N1- and N3-protons of at tRNA's position 39
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Grazyna Czerwinska, Mufeed M. Basti, Andrzej Malkiewicz, Robert Cain, Ghazala Ansari, Connie Yarian, Paul F. Agris, Richard H. Guenther, and Elzbieta Sochacka
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Magnetic Resonance Spectroscopy ,Stereochemistry ,Base pair ,Genes, Fungal ,Biology ,Ribosome ,Pseudouridine ,RNA, Transfer, Phe ,Structure-Activity Relationship ,chemistry.chemical_compound ,RNA, Ribosomal, 16S ,Yeasts ,Anticodon ,otorhinolaryngologic diseases ,Genetics ,30S ,Uridine ,Temperature ,Translation (biology) ,Nuclear magnetic resonance spectroscopy ,Ribosomal RNA ,Biochemistry ,chemistry ,Transfer RNA ,Nucleic Acid Conformation ,Thermodynamics ,Protons ,Ribosomes ,Research Article - Abstract
Pseudouridine at position 39 (Psi(39)) of tRNA's anticodon stem and loop domain (ASL) is highly conserved. To determine the physicochemical contributions of Psi(39)to the ASL and to relate these properties to tRNA function in translation, we synthesized the unmodified yeast tRNA(Phe)ASL and ASLs with various derivatives of U(39)and Psi(39). Psi(39)increased the thermal stability of the ASL (Delta T (m)= 1.3 +/- 0.5 degrees C), but did not significantly affect ribosomal binding ( K (d)= 229 +/- 29 nM) compared to that of the unmodified ASL (K (d)= 197 +/- 58 nM). The ASL-Psi(39)P-site fingerprint on the 30S ribosomal subunit was similar to that of the unmodified ASL. The stability, ribosome binding and fingerprint of the ASL with m(1)Psi(39)were comparable to that of the ASL with Psi(39). Thus, the contribution of Psi(39)to ASL stability is not related to N1-H hydrogen bonding, but probably is due to the nucleoside's ability to improve base stacking compared to U. In contrast, substitutions of m(3)Psi(39), the isosteric m(3)U(39)and m(1)m(3)Psi(39)destabilized the ASL by disrupting the A(31)-U(39)base pair in the stem, as confirmed by NMR. N3-methylations of both U and Psi dramatically decreased ribosomal binding ( K (d)= 1060 +/- 189 to 1283 +/- 258 nM). Thus, canonical base pairing of Psi(39)to A(31)through N3-H is important to structure, stability and ribosome binding, whereas the increased stability and the N1-proton afforded by modification of U(39)to Psi(39)may have biological roles other than tRNA's binding to the ribosomal P-site.
- Published
- 1999
12. NMR and Paramagnetic Ion Substitution Locates a Modified-Nucleoside Dependent Metal Binding Site in DNA: Molecular Dynamics, Surface Charge and H2O Ordering
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Richard H. Guenther, Paul F. Agris, Mufeed M. Basti, and J.W. Stuart
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Transfer DNA ,Crystallography ,chemistry.chemical_compound ,Molecular dynamics ,chemistry ,Proton NMR ,General Materials Science ,Titration ,Metal Binding Site ,General Chemistry ,Surface charge ,Binding site ,DNA - Abstract
The NMR-derived structure, dynamics and metal binding of a unique 17-residue DNA stem and loop hairpin were determined. The hairpin binds one Mg2+ ion and is a biologically active DNA analogue of the yeast tRNAPhe anticodon domain only when two naturally occurring modified nucleosides, d(m5C) and d(m1G), are site selectively incorporated. The modified nucleosides aided in signal assignments in lieu of isotope labeling. Signal assignments were accomplished for 80% of the exchangeable and non-exchangeable protons. Titration of the DNA with Mn2+, monitored by 1H NMR spectroscopy, indicated that the Mg2+ binding site is located in the loop of the hairpin at the bottom of the stem. The binding of Mg2+ contributed to loop stability. Loop dynamics were evidenced by line broadness on the methyl signal of d(m1G) and were compared with that of an identical tDNA stem and loop hairpin that lacked the d(m1G) modification but contained d(m5U). TDNA hairpin dynamics are discussed in the light of the Mg2+ binding constants for the two tDNAPhe. A highly refined, Mg2+-bound structure of the tDNAACPhe is presented along with computer-simulated effects of Mg2+ on the tDNA's net surface charge and surface of H2O.
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- 1996
13. Structure of the Trinucleotide D-acp3U-A with Coordinated Mg2+Demonstrates that Modified Nucleosides Contribute to Regional Conformations of RNA
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Barbara Nawrot, Mufeed M. Basti, Wanda S. Smith, Richard H. Guenther, Brian Forrest, J.W. Stuart, Andrzej Malkiewicz, Paul F. Agris, and Hanna Sierzputowska-Gracz
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chemistry.chemical_classification ,Circular dichroism ,Stereochemistry ,Trimer ,Ribonucleoside ,Biochemistry ,chemistry.chemical_compound ,Crystallography ,chemistry ,Ribose ,Transfer RNA ,Genetics ,Nucleotide ,Dihydrouridine ,Nucleoside - Abstract
In tRNA crystal structures, the only nonaromatic ribonucleoside, dihydrouridine (D) and 3′-adjacent nucleotides adopt the infrequent 2′-endo conformation. Analysis of D, DUA and UUA by circular dichroism (CD) and NMR confirmed that D produces the 2′-endo conformation of the trimer and is responsible for the same in tRNA. The nucleoside, 3-[3-(S)-amino-3-carboxypropyl]uridine (acp3U) occurs in the tRNA sequence D-acp3U-A. CD spectra indicated that D-acp3U-A and U-acp3U-A bind Mg2+, whereas acp3U, D-acp3U, DUA and UUA do not. Ion dependent changes in chemical shifts and paramagnetic broadening of 1H signals indicated that Mg2+ coordination involved the acp3U side chain and the ribose of A. The Mg2+-bound structure was modeled with simulated annealing, molecular mechanics and NMR restraints. Acp3U contributed to local charge density that facilitated Mg2+ coordination. #Present addresses: W. W. S., NMR Facility University of California, Davis, CA; B. F., School of Medicine, University of North Caroli...
- Published
- 1996
14. Ribosome-independent anticodon to codon binding assessed by circular dichroism: Roles of base modifications, Mg2+ and 2′OH
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Paul F. Agris, Richard H. Guenther, Vivian Dao, and Mufeed M. Basti
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Crystallography ,Circular dichroism ,Chemistry ,Transfer RNA ,Base (exponentiation) ,Ribosome ,General Biochemistry, Genetics and Molecular Biology - Published
- 1996
15. Design, biological activity and NMR-solution structure of a DNA analogue of yeast tRNAPhe anticodon domain
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J.W. Stuart, Richard H. Guenther, Paul F. Agris, A.T. Lam, and Mufeed M. Basti
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Magnetic Resonance Spectroscopy ,Base Sequence ,Molecular Structure ,Stereochemistry ,Base pair ,Molecular Sequence Data ,Fungal genetics ,RNA, Fungal ,Translation (biology) ,Saccharomyces cerevisiae ,Nuclear magnetic resonance spectroscopy ,Biology ,Biochemistry ,Ribosome ,RNA, Transfer, Phe ,chemistry.chemical_compound ,chemistry ,Structural Biology ,Transfer RNA ,Anticodon ,Genetics ,A-DNA ,DNA, Fungal ,DNA - Abstract
Design of biologically active DNA analogues of the yeast tRNA(Phe) anticodon domain, tDNAPheAC, required the introduction of a d(m5C)-dependent, Mg(2+)-induced structural transition and the d(m1G) disruption of an intra-loop dC.dG base pair. The modifications were introduced at residues corresponding to m5C-40 and wybutosine-37 in tRNA(Phe). Modified tDNAPheAC inhibited translation by 50% at a tDNAPheAC:ribosome ratio of 8:1. The molecule's structure has been determined by NMR spectroscopy and restrained molecular dynamics with an overall r.m.s.d. of 2.8 A and 1.7 A in the stem, and is similar to the tRNA(Phe) anticodon domain in conformation and dimensions. The tDNAPheAC structure may provide a guide for the design of translation inhibitors as potential therapeutic agents.
- Published
- 1996
16. Transfer of arsenite from glutathione to dithiols: A model of interaction
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Marielle Delnomdedieu, David J. Thomas, Mufeed M. Basti, and James D. Otvos
- Subjects
chemistry.chemical_classification ,Magnetic Resonance Spectroscopy ,Arsenites ,Chemistry ,Dithioerythritol ,Arsenate ,Dithiol ,chemistry.chemical_element ,General Medicine ,Glutathione ,Metabolism ,Toxicology ,chemistry.chemical_compound ,Models, Chemical ,Biochemistry ,Thiol ,Arsenates ,Sulfhydryl Compounds ,Succimer ,Oxidation-Reduction ,Arsenic ,Arsenite - Abstract
The interactions of arsenate and arsenite with meso-2,3-dimercaptosuccinic acid (DMSA) have been characterized using carbon-13 nuclear magnetic resonance. These studies show that DMSA reduces arsenate to arsenite and complexes arsenite. Monitoring the carbon-13 signals of complexed DMSA and liberated glutathione shows that DMSA readily extracts arsenite from a (glutathione)3-arsenite complex, proving the affinity of arsenite for dithiols is greater than that for monothiols. Competition between DMSA (vicinal thiols) and dithioerythritol (1,4-dimercapto-2,3-butanediol) for binding of arsenite indicates that the binding affinity is inversely related to the distance between the two thiol groups. On the basis of these findings, a model for the interaction of arsenic with mono- and dithiol-containing molecules is proposed.
- Published
- 1993
17. 1H-, 13C- and 31P-NMR studies of dioctanoylphosphatidylcholine and dioctanoylthiophosphatidylcholine
- Author
-
Laurine A. LaPlanche and Mufeed M. Basti
- Subjects
Carbon Isotopes ,Magnetic Resonance Spectroscopy ,Chemical Phenomena ,Chemistry, Physical ,Stereochemistry ,Chemical shift ,Organic Chemistry ,Molecular Conformation ,Phospholipid ,Thio ,Phosphorus ,Cell Biology ,Nuclear magnetic resonance spectroscopy ,Biochemistry ,Solvent ,chemistry.chemical_compound ,Crystallography ,chemistry ,Phosphatidylcholine ,Phosphatidylcholines ,Molecule ,Protons ,Molecular Biology ,Micelles ,Vicinal - Abstract
Coupling constants and chemical shifts were measured for dioctanoylphosphatidylcholine and its thio analogue in a CDCl3/CD3OD solvent mixture. Replacing the bridging oxygen atom of the CH—CH2—O—P portion of the phosphatidylcholine molecule with a sulfur atom affects chemical shifts and coupling constants in the glycerol backbone portion of the molecule as well as in the choline head group region. Preferred conformations about selected bonds in the phospholipids were determined from the vicinal 1H-1H, 31P-1H and 31P-13C coupling constants. A reduction of the 31P T 2 ∗ (effective spin-spin relaxation time) for the thio analogue, as well as changes in the relative chemical shifts of 13C nuclei in the acyl chains, suggest a somewhat greater degree of aggregation for the thio analogue. The quadrupolar coupling constant 1J(14N-13C) for the choline methyls of either analogue, however, indicates that aggregation of these phospholipids in the CDCl3/CD3OD solvent mixture is not significant. Differences in conformation between dioctanoylphosphatidylcholine and its thio analogue may be responsible for their differences in chemical and physical properties.
- Published
- 1990
18. [Laparoscopic deroofing of non parasitic posterolateral liver cyst. Technical considerations]
- Author
-
E, Monteferrante, N, Pitrelli, F, Ciarelli, E, Colangelo, M, Basti, M, Nardi, E, Liberatore, and G, Colecchia
- Subjects
Cysts ,Liver Diseases ,Humans ,Laparoscopy - Abstract
Laparoscopic deroofing of the solitary non parasitic cysts of the liver is a safe and effective procedure. This technique allows a wide access for surgical treatment of cysts localized in segments II, III, IVb, V, and VIII of the liver. On the contrary, the posterior segments, VI and VII, and the segment IVa, are difficult to approach laparoscopically. This report describes a laparoscopic technique used in treating 3 cases of symptomatic solitary cysts of the posterior segments of the liver. The patients were placed in the left lateral position. The fenestration treatment was easy and the mobilization of the right hepatic lobe was not required. At one year follow-up, neither postoperative complications nor recurring episodes were observed.
- Published
- 2003
19. A long-term (1965–2015) ecological marine database from the LTER-Italy Northern Adriatic Sea site: plankton and oceanographic observations
- Author
-
F. Acri, M. Bastianini, F. Bernardi Aubry, E. Camatti, A. Boldrin, C. Bergami, D. Cassin, A. De Lazzeri, S. Finotto, A. Minelli, A. Oggioni, M. Pansera, A. Sarretta, G. Socal, and A. Pugnetti
- Subjects
Environmental sciences ,GE1-350 ,Geology ,QE1-996.5 - Abstract
In this paper we describe a 50-year (1965–2015) ecological database containing data collected in the northern Adriatic Sea (NAS) at one of the 25 research parent sites belonging to the Italian Long Term Ecological Research Network (LTER-Italy, http://www.lteritalia.it, last access: February 2020). LTER-Italy is a formal member of the International (https://www.ilter.network, last access: February 2020) and European (http://www.lter-europe.net/, last access: February 2020) LTER networks. The NAS is undergoing a process, led by different research institutions and projects, of establishing a marine ecological observatory, building on the existing facilities, infrastructure, and long-term ecological data. During this process, the implementation of open-access and open-science principles has been started by creating an open-research life cycle that involves sharing ideas and results (scientific papers), data (raw and processed), metadata, methods, and software. The present data paper is framed within this wider context. The database is composed of observations on abiotic parameters and phytoplankton and zooplankton abundances, collected during 299 cruises in different sampling stations, in the Gulf of Venice in particular. Here we describe the sampling and analytical activities, the parameters, and the structure of the database. The database is available at https://doi.org/10.5281/zenodo.3516717 (Acri et al., 2019), and it was also uploaded in the DEIMS-SDR repository (Dynamic Ecological Information Management System – Site and Dataset Registry, https://deims.org/), which is the official site and data registry for the International LTER network.
- Published
- 2020
- Full Text
- View/download PDF
20. [Splenectomy for hematologic disease. Mini-invasive versus traditional technique]
- Author
-
E, Monteferrante, A, Giunta, L, Bigi, G, Colecchia, E, Della Valle, S, Bonacini, C, Pedrazzoli, F, Ciarelli, E, Colangelo, E, Liberatore, M, Nardi, M, Basti, and R, Prati
- Subjects
Adult ,Male ,Splenectomy ,Humans ,Minimally Invasive Surgical Procedures ,Female ,Prospective Studies ,Hematologic Diseases - Abstract
This study aimed to compare the safety, efficacy and clinical benefits of laparoscopic splenectomy (LS) to open splenectomy (OS) in patients with hematologic disorders.prospective study;II Department of Surgery, Santa Maria Nuova Hospital, Reggio Emilia and III Department of Surgery, Santo Spirito Hospital Pescara;48 consecutive adult patients underwent splenectomy; 30 patients under-went LS and 18 OS. Perioperative characteristics, outcomes, complications and costs were comparatively analyzed.Mean age was 35.3 years in the LS group, and 40.8 in the OS group. Mean spleen size was 11.7 cm in the LS group and 15.2 cm in the OS group. Accessory spleens were found in 5 patients in the LS group and in 4 patients in the OS group; 4 conversions to laparotomy occurred in the LS group. A total of 4 complications occurred in 3 patients of the LS; 9 complications occurred in 5 patients of OS group. Mean surgical time was 141.5 minutes for LS and 89.7 minutes for OS (p0.005). Mean postsurgical stay was 5.8 days in the LS group and 8.5 days in the OS group (p0.005). Response rates were similar in both groups.LS is comparable to OS in terms of efficacy and safety and it is associated with a shorter hospital stay. LS should become the technique of choice for treatment of intractable benign hematologic disease.
- Published
- 2001
21. [Laparoalloplasty by the Rives' technique for the treatment of large ventral hernias. Ten-year experience]
- Author
-
G, D'Amico, M, Nardi, M, Basti, E, Casciani, and P, Di Luzio
- Subjects
Male ,Humans ,Female ,Laparoscopy ,Surgical Mesh ,Hernia, Ventral ,Follow-Up Studies - Abstract
The problems of the surgery of bulky laparoceles began to find concrete solutions with the introduction of materials of synthesis that can be used to reinforce or to substitute the abdominal wall. Despite that, the ideal prosthesis has still not been found, the Dacron (Mersilene), the Polypropylene (Marlex, Prolene) and PTFE (Goretex) are today considered the best materials for the alloplasty. The authors describe their experience in matter of bulky laparocels treated with the technique of Rives. 49 patients have been operated, using Dacron in 44 cases and Polypropylene in 5. They had no mortality and no relapse, while the most frequent complication was 6 seromas, all resolved with conservative therapy. In their series 3 infections of the subcuticular occurred more of which required the removal of the prosthesis.
- Published
- 1998
22. [Cancer of the gallbladder]
- Author
-
M, Nardi, G, D'Amico, M, Basti, E, Casciani, P, Di Luzio, and E, Marchese
- Subjects
Aged, 80 and over ,Male ,Carcinoma ,Palliative Care ,Adenocarcinoma ,Middle Aged ,Adenocarcinoma, Papillary ,Chemotherapy, Adjuvant ,Lymphatic Metastasis ,Humans ,Lymph Node Excision ,Cholecystectomy ,Female ,Gallbladder Neoplasms ,Aged ,Neoplasm Staging - Abstract
Carcinoma of the gallbladder is the most frequent neoplasia of the biliary tract with an incidence of 2.5-4.4 cases for every 100,000 inhabitants; it more often affects females and elderly patients (60-70 years). It is an extremely malignant tumour with an often fatal prognosis owing to both its site and the generally late diagnosis; the majority of patients in fact undergo surgery at an advanced stage of the disease when surgery is only palliative. At the Surgical Division of S. Massimo Hospital in Penne (PE) a total of 897 operations on the gallbladder and biliary tract were performed between 1988 and 1994, revealing 21 cases of carcinoma of the cholecyst (2.3%). Sixteen patients were female (male-female ratio 1:3.2) with a mean age of 67.8 years (range 58-83 years). These patients were divided into 5 stages according to the UICC-1992 classification: 80.9% of cases were stage 3-4. Diagnosis was performed intraoperatively in 2 patients, whereas in 3 cases cancer had been diagnosed by the anatomo-pathologist. A total of 3 simple cholecystectomies were performed, 4 were associated with hepatic resection and lymphadenectomy of the hilus and hepatoduodenal ligament; surgery was enlarged in 2 cases due to the involvement of adjacent organs. After exploratory laparotomy, palliative surgery was performed in the remaining 12 patients in the form of biliary and derivation. Four system were implanted for intra-arterial chemotherapy. The most frequently found histological type was adenocarcinoma (76.2%). The mean survival rate of patients who died was 7.6 months. Six of the operated patients are still alive, of whom only one with a follow-up of 4 years. In conclusion, the authors agree with those who consider carcinoma of the gallbladder generally fatal in view of the rarity of early diagnosis and the difficulties of performing radical therapy.
- Published
- 1997
23. Unconventional structure of tRNA(Lys)SUU anticodon explains tRNA's role in bacterial and mammalian ribosomal frameshifting and primer selection by HIV-1
- Author
-
P F, Agris, R, Guenther, P C, Ingram, M M, Basti, J W, Stuart, E, Sochacka, and A, Malkiewicz
- Subjects
Models, Molecular ,Magnetic Resonance Spectroscopy ,Oligoribonucleotides ,Frameshifting, Ribosomal ,DNA-Directed DNA Polymerase ,RNA, Transfer, Glu ,Species Specificity ,Anticodon ,Escherichia coli ,HIV-1 ,Humans ,Nucleic Acid Conformation ,RNA, Transfer, Lys ,Computer Simulation ,Research Article - Abstract
Transfer RNA(Lys)SUU, with a 5-modified-2-thiouridine at wobble position 34, facilitates -1 frameshifts for correct translation of the Escherichia coli DNA polymerase gamma subunit and retroviral polymerases. Peptidyl-tRNA(Lys)SUU prematurely terminates translation more often than other tRNAs. In order to determine if the anticodon structures of bacterial and mammalian tRNA(Lys)SUU species explain these observations, oligonucleotides corresponding to the anticodon regions of mammalian and E. coli tRNA(Lys)SUU were synthesized and their physicochemical properties compared with that of E. coli tRNA(Glu)SUC. The anticodon region of tRNA(Lys)SUU was stabilized by an unusual interaction between the side chains of the 5-modified-s(2)U34 and N-6-threonylcarbamoyl-adenosine-37 (t(6)A37), a combination of modified nucleosides unique to tRNA(Lys)SUU species. This first observation of modified nucleoside side-chain interactions is analogous to the interactions of amino acid side chains in proteins. The tRNA(Lys)SUU anticodon structure was determined from NMR restraints on model oligonucleotides. With only two of three anticodon bases available for codon pairing, this unconventional anticodon structure is a reasonable explanation for the bacterial and mammalian tRNA(Lys)SUU tendency to frameshift. A two-out-of-three reading of coding triplets also explains the increased rate at which peptidyl-tRNA(Lys)SUU prematurely terminates translation. In addition, modified nucleoside interaction distorts the anticodon loop. The distorted loop is a possible structural determinant for the preferential selection of tRNA(Lys3)SUU as primer of HIV-1 reverse transcriptase in vivo.
- Published
- 1997
24. [The selective use of intraoperative cholangiography in video laparoscopic cholecystectomy]
- Author
-
M, Nardi, M, Basti, E, Casciani, P, Di Luzio, R, Di Mizio, and G, D'Amico
- Subjects
Adult ,Aged, 80 and over ,Male ,Intraoperative Care ,Adolescent ,Video Recording ,Gallstones ,Middle Aged ,Cholecystectomy, Laparoscopic ,Acute Disease ,Cholecystitis ,Humans ,Female ,Pneumoperitoneum, Artificial ,Cholangiography ,Aged - Abstract
Intraoperative cholangiography, a diagnostic method through images introduced in to clinical use in 1932, has recently become a different technical support from that attributed by traditional surgery and it has got a different diagnostic meaning with the introduction of video surgery. The authors used it in 54 of 194 patients submitted to a CVL, always making use of the trans-cystic access, with a percentage of success of 82.6%. It was not possible in 8 patients due to the complete section of the cystic duct during the work (3 cases) and the impossibility of introducing the catheter (5 cases). There is still no common agreement about the opportunity to use IC daily: the authors think selective use is better preceded by a careful clinical laboratory instrumental preoperative study to find patients with the common duct stone. Besides, they are of the opinion that a rigorous surgical technique is fundamental to reduce the lesions of the VBP that there are not in their experience.
- Published
- 1997
25. [A rare case of Meckel's diverticulum perforation]
- Author
-
N, Pitrelli, M, Nardi, G, D'Amico, M, Basti, E, Casciani, P, Di Luzio, and G, Cellini
- Subjects
Diagnosis, Differential ,Meckel Diverticulum ,Ileal Diseases ,Intestinal Perforation ,Acute Disease ,Appendectomy ,Humans ,Female ,Appendicitis ,Child ,Foreign Bodies ,Diverticulitis - Abstract
Meckel's diverticulum is a pathology not rarely found everyday clinical medicine especially when it present with one of its complications. The specific diagnosis is a bit difficult to put because of the low sensibility and specificity of symptoms of diagnostic and instrumental techniques used. The reported case shows a rare type of complication constituted by the phlogosis of Meckel's diverticulum, itself due to an extraneous thing ingested by the patient. The authors discuss the entity of the manifestations of this pathology and highlight every aspects.
- Published
- 1995
26. [Branchial carcinoma]
- Author
-
N, Pitrelli, M, Basti, M, Nardi, G, D'Amico, P, Di Luzio, D, Angelucci, and G, Cellini
- Subjects
Aged, 80 and over ,Male ,Head and Neck Neoplasms ,Humans ,Female ,Branchioma ,Middle Aged ,Aged - Abstract
The Authors report three cases of epidermoidal carcinoma arising from a congenital cyst of the anterolateral region of the neck. This type of tumor, rarely found in daily practice, arises from a degenerate branchial tissue and differential diagnosis often includes nodal involvement from systemic neoplasias. However, diagnostic criteria of such diseases are well defined; furthermore the rarity of branchial carcinoma is opposed to the high frequency of metastatic lymph nodes deriving from tumors with similar histological aspects located in other sites which could alter the real percentage of branchial tumor manifestation.
- Published
- 1995
27. [Giant bladder calculus. Report of a clinical case]
- Author
-
N, Pitrelli, M, Basti, M, Nardi, A, Marrone, M, Vacca, and G, D'Amico
- Subjects
Aged, 80 and over ,Male ,Urinary Bladder Calculi ,Humans ,Aged - Abstract
The authors describe a case of giant vesical calculus in an 89 year old patient. The diagnosis, as in the other cases reported in the international literature, was made after a series of laboratory and instrumental exams, for groups reported by the patient in the lower abdomen. Operated for the removal of the vesical calculus, he was considered healed after a fortnight. The vesical calculus has a mixed composition and it weighs about 380 grams. The revision of the literature shows the various chemical composition of the vesical calculus that does not permit to identify a common aetiology. It is not possible to demonstrate if the formation of this kind of calculus is primary (in the urinary bladder) or if it is proper of the high urinary tract or mixed. The most recurrent causes being various and different confirm the mixed aetiopathologic theory. The diagnosis, often casual, is made thanks to the most common diagnostic exams; as it is difficult to make a complete cystoscopic control because the bladder is almost completely occupied by the lithiasic formation. The surgical therapy consists of a epicystotomy with a following removal of the calculus. In literature, moreover, cases treated with extra-body lithotripsy are not reported, on the contrary ot happens daily for the lithiasis of the high urinary tract.
- Published
- 1995
28. [Laparovideocholecystectomy]
- Author
-
G, D'Amico, M, Basti, M, Nardi, E, Casciani, P, Di Luzio, and G, Cellini
- Subjects
Adult ,Male ,Cholecystectomy, Laparoscopic ,Video Recording ,Humans ,Female ,Middle Aged ,Aged - Abstract
The authors present the guidelines which have been followed to introduce into their own Surgical Division an alternative method to cholelithiasis treatment: laparovideocholecystectomy (l.v.c.). They point out how the directions for use of the new surgical method, in place of the traditional laparectomic technique, must be limited to non-complicated cholelithiasis cases, especially in the initial learning stages. That sets the global "morbility" of the above said l.v.c. beneath the cholecystectomy performed with traditional technique. In addition, they point out the sure economic advantages at social and contributions level arriving at the conclusion that l.v.c. is the best treatment for Gallstones.
- Published
- 1995
29. Importance of modified nucleosides to the structure and function of tRNAs
- Author
-
P F, Agris, A, Malkiewicz, R, Guenther, M, Basti, R, Sengupta, and J, Stuart
- Subjects
Binding Sites ,Chemical Phenomena ,Molecular Structure ,RNA, Transfer ,Chemistry, Physical ,Nucleic Acid Conformation ,Magnesium ,Ribonucleosides - Abstract
Although a few modifications are found in DNA, 93 modified nucleosides have been found in the various RNAs. For the most part, the chemistry and structure that modified nucleosides, individually and in combination, uniquely contribute to DNA or RNA function have yet to be explained. However, there are ten physicochemical contributions that can be attributed to modified nucleosides. Of particular interest is the increasingly documented relationship between the presence of modified nucleosides in tRNAs, and the site and affinity of Mg2+ binding to RNA and its effect on function.
- Published
- 1995
30. [Internal hernias. Description of 2 cases]
- Author
-
M, Nardi, A, Perrone, G, D'Amico, M, Basti, N, Pitrelli, E, Casciani, and G, Cellini
- Subjects
Diagnosis, Differential ,Male ,Intestinal Diseases ,Hernia ,Humans ,Mesentery ,Middle Aged ,Peritoneal Diseases ,Prognosis ,Herniorrhaphy ,Aged - Abstract
The authors describe two cases of recent observed internal-hernia, regarding in the first case the right para-duodenal dimple, and in the second a mesenteric breach. They report the clinic presentation and the diagnostic and therapeutic approach compared with the data deriving from the international literature. A complete clinical classification of the internal-hernia is illustrated showing the different clinical signs. The respective symptomatology that in the initial phases of this pathology is not too evident, shows that the internal hernia should always be held in due consideration at the moment of diagnosis because the consequent mortality due to complications such as intestinal-gangrene is rather high.
- Published
- 1994
31. Complexation of arsenic species in rabbit erythrocytes
- Author
-
Miroslav Styblo, Marielle Delnomdedieu, James D. Otvos, David J. Thomas, and Mufeed M. Basti
- Subjects
Erythrocytes ,Magnetic Resonance Spectroscopy ,chemistry.chemical_element ,Ultrafiltration ,In Vitro Techniques ,Toxicology ,Arsenicals ,Arsenic ,chemistry.chemical_compound ,Hemoglobins ,Adenosine Triphosphate ,Animals ,Sulfhydryl Compounds ,Arsenite ,Radioisotopes ,Arsenate ,General Medicine ,Glutathione ,Ligand (biochemistry) ,Ultrafiltration (renal) ,chemistry ,Biophysics ,Hemoglobin ,Rabbits ,Succimer ,Oxidation-Reduction ,Intracellular - Abstract
The binding of arsenite, As(III), and arsenate, As(V), by molecules in the intracellular compartment of rabbit erythrocytes has been studied by 1H- and 31P-NMR spectroscopy, uptake of 73As, and ultrafiltration experiments. For intact erythrocytes to which 0.1-0.4 mM arsenite was added, direct evidence was obtained for entry of 76% within 1/2 h and subsequent binding of As(III) by intracellular glutathione and induced changes in the hemoglobin structure (NMR), likely due to binding of As(III). These results were compared with the effect of addition of As(V) on intact erythrocytes and revealed that a smaller amount of As(V) (approximately 25%) enters the cells; the main fraction of As(V) enters the phosphate pathway, depletes ATP, and increases Pi. In contrast, As(III) did not affect the ATP level. Both 1H- and 31P-NMR data indicated striking differences between As(III) and As(V) behavior when incubated with rabbit erythrocytes. These differences were confirmed by 73As uptake and binding experiments. meso-2,3-Dimercaptosuccinic acid (DMSA), a dithiol ligand, released glutathione from its arsenite complexes in erythrocytes.
- Published
- 1994
32. [Extraperitonealization of the liver in the Chilaiditi syndrome]
- Author
-
G, D'Amico, A, Perrone, M, Nardi, M, Basti, O, De Berardinis, and P, Di Luzio
- Subjects
Liver ,Colon ,Diaphragm ,Humans ,Female ,Syndrome ,Peritoneal Cavity ,Aged - Abstract
The Chilaiditi syndrome or viscero hepato diaphragmatic interposition is a rare anomaly. The authors describe one case they observed. Even if it is often asymptomatic, it may manifest also by recurrent sub-occlusion or occlusion. Only a few patients need corrective surgical operation. The operation of choice is hepatic extra-peritonealization, which after replacement of the dislocated gastroenteric tract, brings the superior face of the liver again into direct contact with the related diaphragmatic dome.
- Published
- 1994
33. [Cancer of the gastric stump. Our experience]
- Author
-
M, Nardi, G, D'Amico, M, Basti, N, Pitrelli, E, Casciani, and G, Cellini
- Subjects
Aged, 80 and over ,Male ,Time Factors ,Liver Neoplasms ,Stomach ,Adenocarcinoma ,Middle Aged ,Pancreatectomy ,Postoperative Complications ,Gastrectomy ,Stomach Neoplasms ,Humans ,Aged ,Follow-Up Studies - Abstract
The authors discuss their experience of six cases of cancer of the gastric stump observed by them in the period from 1988 to 1992 inclusive. All the patients were operated; five total gastrectomies and a gastric re-resection were effected. In association also the spleen was removed in four patients, in one of them a hepatic metastasectomy and a distal pancreasectomy were effected. In the discussion the problems concerning the diagnostic and therapeutic implications with some references to the more likely etiopathogenetic theories were dealt with.
- Published
- 1994
34. [Lumbar hernia of Grynfeltt's quadrilateral space. Apropos a case]
- Author
-
G, D'Amico, M, Nardi, M, Basti, A, Perrone, E, Casciani, P, Di Luzio, and G, Colecchia
- Subjects
Aged, 80 and over ,Male ,Hernia ,Lumbosacral Region ,Humans ,Herniorrhaphy ,Aged - Abstract
The authors discuss a case of Grynfeltt's quadrilateral lumbar hernia in an 85-year-old patient. These rather rare hernias can be classified as Grynfeltt's hernias of the superior lumbar space, as Petit's inferior lumbar space and as diffused hernias concerning the whole hip. In our case this hernia was located in the superior lumbar space and it was delimited on the upper part by the 12th rib and by the serratus posterior-inferior muscle; in the lower part by the internal oblique muscle and by the lumbar quadratus muscle. Surgical treatment was effected by plastic surgery with synthetic material put in the extra-peritoneum space and fixed by serum-muscular stitches. Postoperative control effected after a month from the operation proves a good keeping of the plastic surgery reconstruction with total disappearance of the hernia.
- Published
- 1993
35. [Latero-terminal anastomosis in colorectal surgery using a mechanical stapler. Technical note]
- Author
-
G, Colecchia, A, Perrone, G, D'Amico, E, Casciani, T, Iarussi, and M, Basti
- Subjects
Postoperative Complications ,Surgical Staplers ,Colon ,Anastomosis, Surgical ,Rectum ,Humans ,Colorectal Neoplasms ,Aged - Abstract
Following a discussion of the various methods of colorectal anastomosis in cancer surgery, the Authors describe a technical variant using a mechanical stapler in a high rectal location. The rapidity of anastomosis as well as the decreased risk of dehiscence, fistula and stenosis are underlined.
- Published
- 1992
36. [The role of angiography in cryptogenic submesocolic digestive tract bleeding]
- Author
-
G, D'Amico, G, Colecchia, A, Perrone, E, Casciani, M, Basti, and A, Merlini
- Subjects
Male ,Colonic Diseases ,Adolescent ,Colon ,Recurrence ,Angiography ,Humans ,Female ,Middle Aged ,Gastrointestinal Hemorrhage ,Aged ,Mesocolon - Abstract
Three cases of chronic gastrointestinal bleeding are described; the Authors emphasize the important role of mesenteric arteriography in establishing site and nature of bleeding. The review of international literature shows a success rate between 41 and 73% of cases studied with this method after negativity of other diagnostic procedures.
- Published
- 1991
37. Non-negative data-driven mapping of structural connections with application to the neonatal brain
- Author
-
E. Thompson, A.R. Mohammadi-Nejad, E.C. Robinson, J.L.R. Andersson, S. Jbabdi, M.F. Glasser, M. Bastiani, and S.N. Sotiropoulos
- Subjects
Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Mapping connections in the neonatal brain can provide insight into the crucial early stages of neurodevelopment that shape brain organisation and lay the foundations for cognition and behaviour. Diffusion MRI and tractography provide unique opportunities for such explorations, through estimation of white matter bundles and brain connectivity. Atlas-based tractography protocols, i.e. a priori defined sets of masks and logical operations in a template space, have been commonly used in the adult brain to drive such explorations. However, rapid growth and maturation of the brain during early development make it challenging to ensure correspondence and validity of such atlas-based tractography approaches in the developing brain. An alternative can be provided by data-driven methods, which do not depend on predefined regions of interest. Here, we develop a novel data-driven framework to extract white matter bundles and their associated grey matter networks from neonatal tractography data, based on non-negative matrix factorisation that is inherently suited to the non-negative nature of structural connectivity data. We also develop a non-negative dual regression framework to map group-level components to individual subjects. Using in-silico simulations, we evaluate the accuracy of our approach in extracting connectivity components and compare with an alternative data-driven method, independent component analysis. We apply non-negative matrix factorisation to whole-brain connectivity obtained from publicly available datasets from the Developing Human Connectome Project, yielding grey matter components and their corresponding white matter bundles. We assess the validity and interpretability of these components against traditional tractography results and grey matter networks obtained from resting-state fMRI in the same subjects. We subsequently use them to generate a parcellation of the neonatal cortex using data from 323 new-born babies and we assess the robustness and reproducibility of this connectivity-driven parcellation.
- Published
- 2020
- Full Text
- View/download PDF
38. Single-sided NMR for the measurement of the degree of cross-linking and curing
- Author
-
N. Halmen, C. Kugler, E. Kraus, B. Baudrit, T. Hochrein, and M. Bastian
- Subjects
Technology - Abstract
The degree of cross-linking and curing is one of the most important values concerning the quality of cross-linked polyethylene (PE-X) and the functionality of adhesives and resin-based components. Up to now, the measurement of this property has mostly been time-consuming and usually destructive. Within the shown work the feasibility of single-sided nuclear magnetic resonance (NMR) for the non-destructive determination of the degree of cross-linking and curing as process monitoring was investigated. First results indicate the possibility of distinguishing between PE-X samples with different degrees of cross-linking. The homogeneity of the samples and the curing kinetics of adhesives can also be monitored. The measurements show good agreement with reference tests (wet chemical analysis, differential scanning calorimetry, dielectric analysis). Furthermore, the influence of sample temperature on the characteristic relaxation times can be observed.
- Published
- 2018
- Full Text
- View/download PDF
39. Vulnerability of mixotrophic algae to nutrient pulses and UVR in an oligotrophic Southern and Northern Hemisphere lake
- Author
-
P. Carrillo, J. M. Medina-Sánchez, M. Villar-Argaiz, F. J. Bullejos, C. Durán, M. Bastidas-Navarro, M. S. Souza, E. G. Balseiro, and B. E. Modenutti
- Subjects
Medicine ,Science - Abstract
Abstract Nutrient inputs and ultraviolet radiation (UVR) are global factors affecting the structure and functioning of aquatic ecosystems, particularly clear-water ecosystems. We performed experiments in two model lakes highly exposed to UVR fluxes in order to test the effect that future increases in mineral nutrients transported by dust aerosol might exert on primary producers depending on the likelihood of atmospheric inputs. Lake La Caldera (Northern Hemisphere) has been receiving recurrent dust inputs from the Sahara Desert while lake Los Cántaros (Southern Hemisphere) has been less affected by dust aerosol. UVR × Nutrient synergistically stimulated primary production (PP), chlorophyll a (Chl a), with a smaller increase in phytoplanktonic biomass in La Caldera, but not in Los Cántaros, where nutrient addition unmasked the UVR inhibitory effect on phytoplankton. The proportional decrease of mixotrophic nanoflagellates (MNFs) after the nutrient pulse (in Los Cántaros) and the long-term decline of MNFs in La Caldera associated with the increase in aerosol-dust intrusions from the Sahara during the last 40 years suggest that a future scenario of intensified aerosol events from desert and desertified areas would not only reduce functional diversity with the decline of MNFs, but would ultimately alter the C flux towards the grazing chain in oligotrophic ecosystems.
- Published
- 2017
- Full Text
- View/download PDF
40. Identifying drivers of fox and cat faecal deposits in kitchen gardens in order to evaluate measures for reducing contamination of fresh fruit and vegetables
- Author
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M. Bastien, A. Vaniscotte, B. Combes, G. Umhang, V. Raton, E. Germain, I. Villena, D. Aubert, F. Boué, and M.-L. Poulle
- Subjects
Infectious and parasitic diseases ,RC109-216 - Abstract
Preventing foodborne pathogen contamination of raw fruit and vegetables in the field is critically important for public health. Specifically, it involves preventing faecal deposit by wildlife or domestic animals in fields of crops and kitchen gardens. The present study aims to identify the drivers of fox, dog and cat faecal deposits in kitchen gardens in order to mitigate the risk of contamination of raw produce with parasites shed in carnivore faeces. The focus was on Echinococcus multilocularis, ranked highest in the importance of foodborne parasites in Europe, but attention was also paid to other parasites of major concern - Toxoplasma gondii and Toxocara spp. During the winters of 2014 to 2016, faecal samples were collected from 192 kitchen gardens located in north-eastern France. From these samples, 77% contained scat of carnivores. Molecular analyses revealed that 59% of the 1016 faeces collected were from cats, 31% from foxes, and 10% from dogs. The ease of accessibility to kitchen gardens, the presence of food in the vicinity, and the composition of the surrounding vegetation were used to explain the distribution of fox and cat faeces. Generalized Linear Mixed Effects modelling showed that: i) fencing was not efficient in reducing cat faecal deposits, but drastically decreases those of foxes; ii) the abundance of Microtus sp. indicates a reason for the presence of both fox and cat faecal deposits, iii) the abundance of Arvicola terrestris, the proximity of fruit trees or farms and the predominance of forest and grassland around the village are all drivers of fox faecal deposits. These results point to the importance of fencing around kitchen gardens located in E. multilocularis endemic areas, particularly those surrounded by forest and grassland or close to fruit trees or farms. Keywords: Environmental contamination, Foodborne parasites, Echinococcus multilocularis, Toxoplasma gondii, Toxocara sp.
- Published
- 2019
- Full Text
- View/download PDF
41. Cáncer de próstata: concordancia entre PET 18F-colina y TC en recaída bioquímica
- Author
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J. Hernández Pinzón, C. Ferrarotti, L. Ferrari, D. Mena, N. Larrañaga, J.C. Gallo, and M. Bastianello
- Subjects
Cáncer de próstata ,Colina ,Estadificación TNM ,Tomografía computada ,Tomografía por emisión de positrones ,Medical physics. Medical radiology. Nuclear medicine ,R895-920 - Abstract
Objetivo: Establecer la concordancia de la tomografía por emisión de positrones (PET) con 18-flúor colina y la tomografía computada (TC) para la reestadificación ganglionar (N) y metastásica (M) del TNM en la recaída bioquímica del cáncer de próstata. Materiales y métodos: Se revisaron retrospectivamente historias clínicas de pacientes atendidos en Imágenes moleculares. En cada método establecimos la clasificación TNM ganglionar y metastásica. Se utilizó el índice de concordancia Kappa, categorizando los resultados según lo propuesto por Landis y Koch. Resultados: De los 32 pacientes con PET-colina y TC, en la clasificación ganglionar con PET-colina, 19 (59,4%) fueron N0 y 13 (40,6%), N1; mientras que en la TC, 28 (87,5%) fueron N0 y 4 (12,5%), N1. En la clasificación metastásica, el método PET-colina identificó M0 en 17 (53,1%) pacientes, M1a en 1 (3,1%), M1b en 5 (15,6%), M1c en 1 (3,1%), M1a + M1b en 7 (21,9%), M1b + M1c en 1 (3,1%); mientras que la TC reportó M0 en 23 (71,9%), M1a en 2 (6,25%), M1a + M1b en 2 y M1b + M1c en 5 (15,6%). La concordancia en la clasificación TNM ganglionar y metastásica entre PET-colina y TC fue de 71,88% con 0,3455 de Kappa (error estándar: 0,1336; p= 0,0049) y de 62,5% con 0,3725 de Kappa (error estándar: 0,0847; p = 0,0001), respectivamente. Discusión: El método PET-colina demostró alta exactitud diagnóstica en la extensión de la enfermedad, con respecto a los métodos convencionales. Conclusión: La concordancia entre el método PET-colina y la TC para la clasificación TNM ganglionar y metastásica no es buena.
- Published
- 2016
- Full Text
- View/download PDF
42. Flux measurements by the NRC Twin Otter atmospheric research aircraft: 1987–2011
- Author
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R. L. Desjardins, D. E. Worth, J. I. MacPherson, M. Bastian, and R. Srinivasan
- Subjects
Science ,Physics ,QC1-999 ,Meteorology. Climatology ,QC851-999 - Abstract
Over the past 30 years, the Canadian Twin Otter research group has operated an aircraft platform for the study of atmospheric greenhouse gas fluxes (carbon dioxide, ozone, nitrous oxide and methane) and energy exchange (latent and sensible heat) over a wide range of terrestrial ecosystems in North America. Some of the acquired data from these projects have now been archived at the Flight Research Laboratory and Agriculture and Agri-Food Canada. The dataset, which contains the measurements obtained in eight projects from 1987 to 2011 are now publicly available. All these projects were carried out in order to improve our understanding of the biophysical controls acting on land-surface atmosphere fluxes. Some of the projects also attempted to quantify the impacts of agroecosystems on the environment. To provide information on the data available, we briefly describe each project and some of the key findings by referring to previously published relevant work. As new flux analysis techniques are being developed, we are confident that much additional information can be extracted from this unique data set.
- Published
- 2016
- Full Text
- View/download PDF
43. Molecular conformations of dibucaine in solution as determined by NMR lanthanide—induced shifts and conformational energy calculations
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Mufeed M. Basti, Laurine A. LaPlanche, and Garret Vanderkooi
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Steric effects ,Tertiary amine ,Stereochemistry ,Quinoline ,Dibucaine ,General Chemistry ,Nuclear magnetic resonance spectroscopy ,Ring (chemistry) ,chemistry.chemical_compound ,Crystallography ,chemistry ,Amide ,medicine ,Molecule ,General Materials Science ,medicine.drug - Abstract
Proton lanthanide-induced shifts (LIS) were measured for Yb(fod)3-dibucaine complexes in CDCl3 solution. The data were used in conjunction with empirical energy calculations to determine the preferred conformations of dibucaine. The energy calculations and the LIS analysis were in agreement in showing that the plane of the amide group makes an angle of approximately 60° with the plane of the quinoline ring, and also that the two carbon-carbon bonds between the amide group and the tertiary amine nitrogen are both in gauche configurations. Energy calculations showed that the diethyl tertiary zmine group has very restricted conformational freedom owing to internal steric constraints. The butyl ether group has considerable flexibility but is forced out of the plane of the quinoline ring by steric effects.
- Published
- 1987
44. Estimation of Wear Behavior of Polyphenylene Sulphide Composites Reinforced with Glass/Carbon Fibers, Graphite and Polytetrafluoroethylene, by Pin-on-disc Test
- Author
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M.A.C. Besnea, D.C. Trufasu, G. Andrei, M. Bastiurea, and M.S. Rodeanu
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Polyphenylene sulphide ,Glass/carbon fiber Polytetrafluorethylene ,Wear ,Mechanical engineering and machinery ,TJ1-1570 - Abstract
Wear behavior of polyphenylene sulphide composites was investigated according to load and test speed. Two types of materials were studied: first, with 40 wt% glass fiber, and second, with 10 wt% carbon fiber, 10 wt% graphite and 10 wt%. Tribological tests were performed on the universal tribometer UMT-2, using a pin-on-disc device. The friction coefficient and wear rate for the composites were analyzed. As a result of experimental tests, it was established that polymer composite with polyphenylene sulphide matrix, carbon fibers, graphite and polytetrafluorethylene exhibit good wear behavior under operating conditions.
- Published
- 2015
45. Determination of Specific Heat of Polyester Composite with Graphene and Graphite by Differential Scanning Calorimetry
- Author
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M. Bastiurea, M.S. Rodeanu Bastiurea, G. Andrei, D. Dima, M. Murarescu, M. Ripa, and A. Circiumaru
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Polyester ,Composite ,Graphene ,Graphite ,Specific heat ,Mechanical engineering and machinery ,TJ1-1570 - Abstract
Some polymers show low thermal properties which can be improved by adding various types of materials. Using nanoparticles, an enhancement of thermal properties can be obtained, even for small contents of additives. The evaluation of thermal properties of polymer composites with graphene can be achieved relying on Differential Scanning Calorimetry tests (DSC). This work presents a few conclusions resulting from DSC tests of the polyester composites with graphene and graphite.
- Published
- 2014
46. Brief communication 'An extreme meteorological event at the ISMAR oceanographic tower'
- Author
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M. Bastianini, L. Cavaleri, and T. La Rocca
- Subjects
Environmental technology. Sanitary engineering ,TD1-1066 ,Geography. Anthropology. Recreation ,Environmental sciences ,GE1-350 ,Geology ,QE1-996.5 - Abstract
We report the evidence of a remarkable meteorological event in the Northern Adriatic Sea. Following the irruption of cold northerly air into the previously hot and humid eastern part of the Po valley, a strong instability developed with violent thunderstorms. At the ISMAR oceanographic tower, 15 km off the coast of the Venice lagoon, although no one was on board, the records of 6 July 2008 from the various instruments coherently show the presence of an extreme and short-lived event that we associate either to a water spout or, more likely, to the strong downdraft of a mesoscale convective system
- Published
- 2012
- Full Text
- View/download PDF
47. Determination of Glass Transition Temperature for Polyester / Graphene Oxide and Polyester / Graphite Composite by TMA and DSC
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M. BAȘTIUREA, M. S. BAȘTIUREA, Gabriel ANDREI, M. MURARESCU, and D. DUMITRU
- Subjects
polyester ,graphene oxide ,graphite ,Tg ,TMA ,DSC ,Mining engineering. Metallurgy ,TN1-997 - Abstract
The influences of oxide grapheme and grapheme in thermosetting polymer composites are complex and they very much depend on the chemical bonds formed between the additives and the polymer matrix. This study has used polyester as polymeric matrix and oxide grapheme and graphite as additives. Determination of glass transition temperature (Tg) is important for practical uses of polyester composites due to the changes of characteristics triggered by transition, thus the polymer passes from elastic to plastic state. In order to determine the Tg we used TMA, DSC, DMA tests. The differences in determined Tg values for the same composite are due to different measurements as resulted from each test.
- Published
- 2015
48. The 'Sima del Elefante' cave site at Atapuerca (Spain)
- Author
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A. Rosas, R. Huguet, A. Pérez-González, E. Carbonell, J. M. Bermúdez de Castro, J. Vallverdú, J. van der Made, E. Allué, N. García, R. Martínez-Pérez, J. Rodríguez, R. Sala, P. Saladie, A. Benito, C. Martínez-Maza, M. Bastir, A. Sánchez, and J. M. Parés
- Subjects
early pleistocene ,middle pleistocene ,human occupation ,mediterranean ecosystem ,paleoecology ,site formation ,Geology ,QE1-996.5 - Abstract
El yacimiento de la Sima del Elefante (TE) (Atapuerca, España) se localiza en el extremo sur de la trinchera del ferrocarril de la Sierra de Atapuerca. TE constituye una sección transversal de una antigua galería kárstica totalmente colmatada de sedimentos pleistocenos. La secuencia estratigráfica completa alcanza los 25 m de potencia y ha sido dividida en 21 unidades lito-estratigráficas delimitadas por discontinuidades mayores. La historia de la cavidad puede ordenarse en al menos tres fases. La fase inferior (niveles TE8 a TE14) data del tercio final del Pleistoceno Inferior, con una cronología entre 1.1 y 1.4 millones de años. Es rica en registro arqueo-paleontológico y presenta claras evidencias de actividad antrópica. Dada su cronología, los niveles inferiores de TE representan un importante referente para la comprensión de la primera colonización humana de Europa. La fase intermedia la constituyen las unidades TE15 a TE19. El tramo basal de esta segunda fase de relleno (TE15 a TE17) hasta la fecha no ha deparado contenido fósil. El tramo superior (TE18 y TE19), cuya cronología parece corresponder a la parte final del Pleistoceno Medio, es rica en restos de grandes mamíferos e industria lítica sobre sílex y cuarcita. La unidad TE19 se caracteriza por una sucesión de coladas detríticas (“debris-flow”) (TE19A a TE19G), con matriz muy dura y carbonatada, rica en huesos de grandes mamíferos y ausencia de micromamíferos. El nivel TE19G contiene abundantes restos de carbón pendiente de verificar su posible carácter antrópico. Finalmente, la tercera y última fase de relleno (TE20 y TE21) corresponde al Pleistoceno superior y se define por la colmatación final de la cavidad y formación de un horizonte edáfico. Los intervalos cronológicos representados en los niveles fértiles de la Sima del Elefante (TE) complementan la secuencia cronológica y bioestratigráfica local del complejo Atapuerca. La fase inferior de TE tuvo lugar en un tiempo anterior a los niveles inferiores de Gran Dolina (TD4), mientras que el relleno de las unidades superiores de TE corresponde a un momento posterior a la sedimentación de GIII/GIV de Galería y TD10/TD11 de Gran Dolina.
- Published
- 2006
- Full Text
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49. Gato de Cheshire
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M. Bastianello, D. Mena, and J.C. Gallo
- Subjects
Medical physics. Medical radiology. Nuclear medicine ,R895-920 - Published
- 2017
- Full Text
- View/download PDF
50. Optimal Short-Time Acquisition Schemes in High Angular Resolution Diffusion-Weighted Imaging
- Author
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V. Prčkovska, H. C. Achterberg, M. Bastiani, P. Pullens, E. Balmashnova, B. M. ter Haar Romeny, A. Vilanova, and A. Roebroeck
- Subjects
Medical physics. Medical radiology. Nuclear medicine ,R895-920 ,Medical technology ,R855-855.5 - Abstract
This work investigates the possibilities of applying high-angular-resolution-diffusion-imaging- (HARDI-) based methods in a clinical setting by investigating the performance of non-Gaussian diffusion probability density function (PDF) estimation for a range of b-values and diffusion gradient direction tables. It does so at realistic SNR levels achievable in limited time on a high-performance 3T system for the whole human brain in vivo. We use both computational simulations and in vivo brain scans to quantify the angular resolution of two selected reconstruction methods: Q-ball imaging and the diffusion orientation transform. We propose a new analytical solution to the ODF derived from the DOT. Both techniques are analytical decomposition approaches that require identical acquisition and modest postprocessing times and, given the proposed modifications of the DOT, can be analyzed in a similar fashion. We find that an optimal HARDI protocol given a stringent time constraint (48). Our findings generalize to other methods and additional improvements in MR acquisition techniques.
- Published
- 2013
- Full Text
- View/download PDF
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