1. LPS-induced systemic inflammation reveals an immunomodulatory role for the prion protein at the blood-brain interface
- Author
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Ø. Salvesen, M. R. Reiten, A. Espenes, M. K. Bakkebø, M. A. Tranulis, and C. Ersdal
- Subjects
Cellular prion protein ,Systemic inflammation ,Lipopolysaccharide (LPS) ,Innate immunity ,Choroid plexus ,Hippocampus ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Abstract Background The cellular prion protein (PrPC) is an evolutionary conserved protein abundantly expressed not only in the central nervous system but also peripherally including the immune system. A line of Norwegian dairy goats naturally devoid of PrPC (PRNP Ter/Ter) provides a novel model for studying PrPC physiology. Methods In order to explore putative roles for PrPC in acute inflammatory responses, we performed a lipopolysaccharide (LPS, Escherichia coli O26:B6) challenge of 16 goats (8 PRNP +/+ and 8 PRNP Ter/Ter) and included 10 saline-treated controls (5 of each PRNP genotype). Clinical examinations were performed continuously, and blood samples were collected throughout the trial. Genome-wide transcription profiles of the choroid plexus, which is at the blood-brain interface, and the hippocampus were analyzed by RNA sequencing, and the same tissues were histologically evaluated. Results All LPS-treated goats displayed clinical signs of sickness behavior, which were of significantly (p
- Published
- 2017
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