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Abstract The World Health Organisation (WHO) presents an upper limit for lead in drinking water of 10 parts per billion ppb. Typically, to reach this level of sensitivity, expensive metrology is required. To increase the sensitivity range of low-cost devices, this paper explores the prospects of using a volume reduction technique of a boiled water sample doped with Lead-210 ( $${}^{210}$$ 210 Pb), as a means to increase the solute’s concentration. $${}^{210}$$ 210 Pb is a radioactive lead isotope and its concentration in a water sample can be measured with e.g. High Purity Germanium (HPGe) detectors at the Boulby Underground Germanium Suite. Concentrations close to the WHO limit have not been examined. This paper presents a measurement of the volume reduction technique retaining $$99\pm (9)$$ 99 ± ( 9 ) % of $${}^{210}$$ 210 Pb starting from a concentration of $$1.9 \times 10^{-6}$$ 1.9 × 10 - 6 ppb before reduction and resulting in $$2.63 \times 10^{-4}$$ 2.63 × 10 - 4 ppb after reduction. This work also applies the volume reduction technique to London tap water and reports the radioassay results from gamma counting in HPGe detectors. Among other radio-isotopes, $${}^{40}$$ 40 K, $${}^{210}$$ 210 Pb, $${}^{131}$$ 131 I and $${}^{177}$$ 177 Lu were identified at measured concentrations of $$2.83 \times 10^{3}$$ 2.83 × 10 3 ppb, $$2.55 \times 10^{-7}$$ 2.55 × 10 - 7 ppb, $$5.06 \times 10^{-10}$$ 5.06 × 10 - 10 ppb and $$5.84 \times 10^{-10}$$ 5.84 × 10 - 10 ppb in the London tap water sample. This technique retained $${90 \pm 50}{\%}$$ 90 ± 50 % of $${}^{40}$$ 40 K. Stable lead was inferred from the same water sample at a measured concentration of 0.012 ppb, prior to reduction.

This paper aims at encouraging the use of laser treatment as an environmentally friendly technique to improve the mechanical properties of metallic materials over conventional quenching and tempering techniques through the study of the tribological behavior of AISI 1538 MV steel subjected to surface laser quenching treatment. Sliding wear tests were carried out by the pin-on-disk method. In order to identify the wear mechanisms, the worn surfaces on the disks were evaluated by scanning electron microscopy and the wear scars on the ball were observed by optical microscopy. Results reveal that laser treatment reduces the average friction coefficient by 25% and the wear rate by 60% compared with those achieved by the conventional methods, while the depths of the wear track and hardness of the cross section and surface are maintained.

Introduction: A current purpose of the medical education is to achieve the prominence of the biomedical subjects in the preparation of the doctors. The suitable motivation is the most adapted way to reach it. In Cuba, the medical teaching has assumed changes with marked incidence in the biomedical subjects. The morphophysiology emerged to integrate with didactic ends the morphological and physiological contents. What created disjunctives about the methods and means for the teaching morphological contents, in front of the tendency to substitute the traditional forms by computer images. It was a problem to resolve by means of the educational search work and methodological scientist. Objective: This work has the aim to value the impact of pedagogical didactic – actions implemented in medicine students of first year to develop cognitive ability in profit of the process of education learning. Methods: The universe was the learning results of the medicine students and the courses among 2008 and 2013 constituted the sample. By random way were taken in each course experimental and control groups. In the first was stimulated the motivation with the method basic on problem, the employment of the optical microscope, histological slides and anatomical pieces. The averages of the experimental groups and controls was compared, in the same manner was compared with total and the accumulated average. Results: The averages of the experimental groups resulted upper to the of the groups control and to the academic average accumulated. Of this way verified the hypothesis posed that the actions implemented in the methodological didactic conception based in the method basic on problem resulted favourable for the development of the cognitive ability of the students. Conclusions: The method basic on problem link with means of traditional education in the morphological sciences, combining them properly with the new means of digitalized images stimulates the motivation, favours the development of cognitive ability and increases the academic results.

Introduction: The methodological work of the department of morfofisiología loans special attention to the quality of the educational process. One of the purposes is the suitable employment of the valued criterion of the professor like a focus of the efficiency of the process, that it is useful to his time also for the prediction of the educational results, in the individual and collective. Objective:The present work studies the correlation of the valued criterion of the professor with the achievement of the aims of the subjects, expressed this last by the educational results obtained in the ordinary examination. It was studied its chronological behavior in the period of five years Material and Methods: A matrix of correlation was created. We determined the coefficient of Spearman. Some factors that could influence in its objectivity predict were also identified by the study. Results:The review of the registers of methodological meetings, open classes and newspapers of classes allowed us to identify some factors that could act on the valued criterion of the professor. They can improve the objectivity of the correlation between this criterion and the academic achieving at the final examination in the subject of molecular biology in the students of first year on medicine career. Conclusions: To attention and systematic monitory of the individual educational evolution and collective in the methodological work of the subject and in the department, attains to increase the efficiency of the valued criterion of the professor and its correlation with the ends academic results. The results constitute an educational innovation for the methodological work in the subjects that integrate the Biological Bases of the Medicine.

ABSTRACT: Improving production efficiency and minimizing the environmental impact of dairy farming are 2 important goals of the dairy industry. Achieving these objectives requires improving the feed-to-milk conversion efficiency. One way to achieve this goal is through genetic selection. However, measuring feed efficiency in commercial herds is currently not feasible. As such, we conducted a study to evaluate the genetic accuracy of various selection indexes derived from Fourier-transform mid-infrared (FTIR) spectra or milk composition. We used 7,793 weekly records on 537 genotyped cows (78,964 SNPs), with information on residual feed intake (RFI) and FTIR spectra. We fitted various types of selection indexes using the complete FTIR spectra of milk samples. The estimated heritability of RFI (± SD) was 0.12 ± 0.02. The accuracy of indirect selection using the FTIR spectra was maximized by using a principal components selection index (0.16 ± 0.07), followed by a least absolute shrinkage and selection operator-type penalized selection index (0.14 ± 0.06). We determined that an index based on milk spectral data recorded on ∼25 daughters produced a progeny average with an accuracy comparable to direct phenotypic selection for RFI. We conclude that indirect selection for RFI using FTIR spectra data can be effective for sires with progeny; however, future studies with a larger sample size are needed to validate these results.

Microplastic, resulting from the breakdown of larger plastic objects, poses substantial threats to ecosystems and human health. This research addresses the escalating environmental concern of airborne microplastics by developing a low-cost detection machine employing infrared sensors. The near-infrared light is attached to the machine for detection using the sensors. Different types of microplastic, specifically less than 5mm, are used. There are 3 types of plastics used for detection, 30 pieces each of polyethylene, polystyrene, and polyester microplastics. Each type of microplastic is tested for detection in different time frames, 1, 2, 3, 4, and 5 minutes each, and the three types of plastics are combined randomly for the last detection with the same number of minutes. Because of the final results, it can determine the effectiveness of the sensor in detecting airborne microplastics in terms of the number of correctly detected microplastics. In conclusion, this research advances our capacity to detect airborne microplastics, contributing to environmental conservation and public health protection. The detection machine's development and the insights gained from the study have broader implications for standardizing methodologies, advancing technology, and fostering collaborative efforts in addressing the challenges posed by airborne microplastics.

This research focused on developing a methodology that facilitates the learning of control engineering students, specifically developing skills to design a complete control loop using fuzzy logic. The plant for this control loop is a direct current motor, one of the most common actuators used by educational and professional engineers. The research was carried out on a platform developed by a group of students. Although the learning techniques for the design and implementation of controllers are extensive, there has been a delay in teaching techniques that are relatively new compared to conventional control techniques. Then, the hands-on laboratory offers a tool for students to acquire the necessary skills in driver tuning. In addition to the study of complete systems, the ability to work in a team is developed, a fundamental skill in the professional industrial area. A qualitative and quantitative analysis of student learning was carried out, integrating a multidisciplinary project based on modern tools.

Abstract Passion fruits species presents a larger economic importance for in natura consumption and industry. The main way to propagate these species is by seeds; however, the aril presence and environmental conditions may inhibit the seeds germination. In this sense, the objective of this study was to evaluate the effect of aril removal methods and light conditions on P. edulis var. flavicarpa seeds quality and germination. There were used five methods to remove the seeds aril: (1) friction on steel mesh sieve for 5 minutes; (2) friction with coarse sand on steel mesh sieve for 5 minutes; (3) immersion in lime solution at 10% (w/v), for 30 minutes; (4) fermentation in sugar solution at 10% (w/v), during 48 hours; and (5) natural pulp fermentation, during five days. The seeds were submitted to germination test at darkness and lightness, as well as, to length seedlings and emergence test. Regardless the aril removal method, the germination percentage was lower at lightness than darkness highlighting the natural pulp fermentation treatment, which presented 86% of seeds germinated. On the other hand, the aril removal methods did not affect the seedlings emergence, with emergence percentages ranged from 88 to 94%.

Abstract In this work, we investigate the violation of Lorentz symmetry through the Casimir effect, one of the most intriguing phenomena in modern physics. The Casimir effect, which represents a macroscopic quantum force between two neutral conducting surfaces, is widely regarded as a triumph of Quantum Field Theory. In this study, we present new results for the Casimir effect, focusing on the contribution of mass associated with fermionic quantum fields confined between two large parallel plates, in the context of Lorentz symmetry violation within the Horava–Lifshitz formalism. To calculate the Casimir energy and pressure, we impose a MIT bag boundary condition on the two plates, compatible with the higher-order derivative term in the modified Dirac equation. Our results reveal a strong influence of Lorentz violation on the Casimir effect. We observe that the Casimir energy is significantly affected, both in intensity and sign, potentially resulting in a repulsive or attractive force between the plates, depending on the critical exponent associated with the Horava–Lifshitz formalism.

Multiple endocrine neoplasia type 2 (MEN2) is a hereditary cancer syndrome caused by germline-activating pathogenic variants in the RET proto-oncogene. MEN2A is the most common subtype, with a risk for medullary thyroid cancer (MTC), pheochromocytoma (PHEO), and primary hyperparathyroidism (PHPT), whereas MEN2B is less common and associated with MTC and PHEO along with mucosal neuromas. Little is known about the specific RET germline heterozygous variant K666N. This variant has been described in very few families, and in most cases, patients were diagnosed with a very indolent MTC as the only feature. There is one case of MTC and bilateral PHEO. The RET K666N variant is not stratified yet by the American Thyroid Association, and data are limited on pathogenicity; therefore, appropriate screening and treatment of asymptomatic RET K666N carriers are unclear. Here, we report a family with a heterozygous germline RET K666N variant. The proband was identified when she experienced cardiogenic shock and multi-organ failure after an elective hysterectomy and subsequently was found to have PHEO, with genetic testing revealing the RET K666N germline variant. Patient consent was obtained through IRB protocol COMIRB #15-0516.

The work shows a methodological procedure that allows to imply the professionals in process of formation of technical specialties and university careers in the evaluation of the process of formation of its professional abilities assuming for it a focus of competitions. For he/she intends a system of indicators with their corresponding scales of valuation that allow to end up dominating the level that the students reach in the formation of the same ones; as well as, the regulation of the one referred process with effectiveness, without ignoring the affective of the personality and the educational of the process.

In the last decades, robotic cultivation facilities combined with automated execution of workflows have drastically increased the speed of research in biotechnology. In this work, we present the design and deployment of a digital infrastructure for robotic cultivation platforms. We implement a Workflow Management System, using Directed Acyclic Graphs, based on the open-source platform Apache Airflow to increase traceability and the automated execution of experiments. We demonstrate the integration and automation of experimental workflows in a laboratory environment with a heterogeneous device landscape including liquid handling stations, parallel cultivation systems, and mobile robots. The feasibility of our approach is assessed in parallel E. coli fed-batch cultivations with glucose oscillations in which different elastin-like proteins are produced. We show that the use of workflow management systems in robotic cultivation platforms increases automation, robustness and traceability of experimental data.

In the present study, manganese (Mn2+), a neuromuscular blocker with pre and postsynaptic actions, was used to verify the neurotoxicity and myotoxicity induced by Crotalus durissus terrificus (Cdt) and Bothrops jararacussu (Bjssu) venoms in biventer cervicis preparations (BCp). Preparations pretreated with 0.66 and 1.6mM Mn2+ did not affect Cdt venom-induced blockage nor change KCl-induced contracture but partially reduced ACh-induced contracture. However, both Mn2+ concentrations partially hindered Bjssu venom-induced blockage after washing the preparations with Krebs solution, and only 1.6mM Mn2+ preparations significantly recovered ACh-induced contracture. The effect of Cdt venom myotoxicity on contractile responses was different from that of Bjssu venom myotoxicity. Pretreatment with 1.6mM Mn2+ partially reduced muscle damage percentage and creatine kinase (CK) activity (U/l) induced by both venoms. In conclusion, Mn2+ interfered in ACh-induced contracture of the nicotinic receptor; did not prevent Cdt venom neurotoxicity but partially reduced its myotoxicity in vitro due to the stabilizing action of this venom on the sarcolemmal membrane; and partially attenuated myotoxicity and neuromuscular blockage induced by Bjssu venom. The Mn2+ dual action (pre and postsynaptic) is useful to study snake venoms since most of them present one or both of these actions; besides, Mn2+ allowed recovering coherent interpretation of experimental versus clinical results.

Members of the genus Scaphanocephalus mature in accipitrids, particularly osprey, Pandion haliaetus, with metacercaria causing Black Spot Syndrome in reef fishes. In most of the world, only the type species, Scaphanocephalus expansus (Creplin, 1842) has been reported. Recent molecular studies in the Western Atlantic, Mediterranean and Persian Gulf reveal multiple species of Scaphanocephalus, but have relied on 28S rDNA, mainly from metacercariae, which limits both morphological identification and resolution of closely related species. Here we combine nuclear rDNA with mitochondrial sequences from adult worms collected in osprey across North America and the Caribbean to describe species and elucidate life cycles in Scaphanocephalus. A new species described herein can be distinguished from S. expansus based on overall body shape and size. Phylogenetic analysis of the whole mitochondrial genome of Scaphanocephalus indicates a close relationship with Cryptocotyle. We conclude that at least 3 species of Scaphanocephalus are present in the Americas and 2 others are in the Old World. Specimens in the Americas have similar or identical 28S to those in the Mediterranean and Persian Gulf, but amphi-Atlantic species are unlikely in light of divergence in cytochrome c oxidase I and the lack of amphi-Atlantic avian and fish hosts. Our results provide insight into the geographic distribution and taxonomy of a little-studied trematode recently linked to an emerging pathology in ecologically important reef fishes.

Summary: Background & aims: A 2-hour postprandial glucose (2-h PG) concentration greater than 140 mg/dL is associated with a greater risk of type-2 diabetes (TD2) and cardiovascular disease. An oral glucose tolerance test (OGTT) is the method most used for detecting either prediabetes or T2D; however, its use in clinical practice is limited due to the 2-h testing period. Therefore, the aim of the study was to assess dysglycaemia, through a prognostic assessment of body composition, biochemical and clinical parameters as predictors of an abnormal 2-h PG. Methods: Participants underwent a physical examination in which their anthropometric measurements were obtained, and a blood sample was taken for biochemical and hormonal analysis. All patients underwent a 75-g OGTT after 8 h of fasting, and the levels of glucose and insulin were measured. The results were compared statistically by dividing the participants' 2-h postprandial glucose (2-h PG) into the following groups: normoglycaemia (NGT),

The integrity of myelin sheaths is maintained by oligodendrocytes and Schwann cells respectively in the central nervous system (CNS) and in the peripheral nervous system. The process of demyelination consisting of the withdrawal of myelin sheaths from their axons is a characteristic feature of multiple sclerosis, the most common human demyelinating disease. Many experimental models have been designed to study the biology of demyelination and remyelination (repair of the lost myelin) in the CNS, due to the difficulties in studying human material. In the ethidium bromide (an intercalating gliotoxic drug) model of demyelination, CNS remyelination may be carried out by surviving oligodendrocytes and/or by cells differentiated from the primitive cell lines or either by Schwann cells that invade the CNS. However, some factors such as the age of the experimental animals, intensity and time of exposure to the intercalating chemical and the topography of the lesions have marked influence on the repair of the tissue.A integridade da bainha de mielina é fornecida pelos oligodendrócitos e pelas células de Schwann, no sistema nervoso central (SNC) e no sistema nervoso periférico, respectivamente. O fenômeno de desmielinização refere-se à remoção das bainhas de mielina de axônios e este fato é característico na esclerose múltipla, a doença desmielinizante do SNC mais comum no homem. Muitos modelos experimentais têm sido utilizados para o estudo da biologia da desmielinização e remielinização no SNC, face à dificuldade de estudo de material humano. No modelo experimental da droga intercalate, gliotóxica, brometo de etídio, a remielinização do SNC pode ser efetuada por oligodendrócitos sobreviventes à lesão e/ou oriundos de diferenciação de linhagens celulares mais primitivas e por células de Schwann que invadem o SNC. No entanto, fatores como a idade dos animais, a intensidade, e o tempo de exposição ao agente intercalante e a topografia da lesão influenciam significativamente a reparação da lesão.

The molecular chaperone heat shock proteins 70 (Hsp70) play a pivotal role in preserving cellular integrity and managing stress. This study extensively examined two Hsp70 proteins, On-Hsp70cBi, inducible, and On-Hsp70cBc, constitutively expressed, in Nile tilapia (Oreochromis niloticus) utilizing in silico analysis, homology modeling, and functional annotation. Employing the SWISS-MODEL program for homology modeling, the proposed models underwent thorough reliability assessment via ProSA, Verify 3D, PROVE, ERRAT, and Ramachandran plot analyses. Key features of On-Hsp70cBi and On-Hsp70cBc included amino acid lengths (640 and 645) and molecular weights (70,233.48 and 70,773.17 Da). Moreover, theoretical isoelectric points (pI = 5.63 and 5.28), indicated their acidic nature. Counts of negatively and positively charged residues (95 and 86; 95 and 81) revealed neutrality, while instability index (II) values of 35.27 (On-Hsp70cBi) and 38.85 (On-Hsp70cBc) suggested stability. Aliphatic index (AI) values were notably high for both proteins (84.58 and 82.85), indicating stability across a broad temperature range. Domain architecture analysis showed both proteins to contain an MreB/Mbl domain. Protein-protein interaction analysis identified the co-chaperone Stip1 as a primary functional partner. Comparative modeling yielded highly reliable 3D models, showcasing structural similarity to known proteins and predicted binding sites. Additionally, both proteins are primarily localized in the cytoplasm. Functional analysis predicted an AMP-PNP binding site for On-Hsp70cBi and an ATP binding site for On-Hsp70cBc. These findings deepened our understanding of Hsp70cBc and Hsp70cBi in Nile tilapia, underscoring their significance in fish physiology and warranting further investigation, thus advancing our knowledge of these proteins' roles in cellular processes and stress responses, potentially impacting fish health and resilience.

Biofilm formation by the pathobiont Haemophilus influenzae is associated with human nasopharynx colonization, otitis media in children, and chronic respiratory infections in adults suffering from chronic respiratory diseases such as chronic obstructive pulmonary disease (COPD). β-lactam and quinolone antibiotics are commonly used to treat these infections. However, considering the resistance of biofilm-resident bacteria to antibiotic-mediated killing, the use of antibiotics may be insufficient and require being replaced or complemented with novel strategies. Moreover, unlike the standard minimal inhibitory concentration assay used to assess antibacterial activity against planktonic cells, standardization of methods to evaluate anti-biofilm drug activity is limited. In this work, we detail a panel of protocols for systematic analysis of drug antimicrobial effect on bacterial biofilms, customized to evaluate drug effects against H. influenzae biofilms. Testing of two cinnamaldehyde analogs, (E)-trans-2-nonenal and (E)-3-decen-2-one, demonstrated their effectiveness in both H. influenzae inhibition of biofilm formation and eradication or preformed biofilms. Assay complementarity allowed quantifying the dynamics and extent of the inhibitory effects, also observed for ampicillin resistant clinical strains forming biofilms refractory to this antibiotic. Moreover, cinnamaldehyde analog encapsulation into poly(lactic-co-glycolic acid) (PLGA) polymeric nanoparticles allowed drug vehiculization while maintaining efficacy. Overall, we demonstrate the usefulness of cinnamaldehyde analogs against H. influenzae biofilms, present a test panel that can be easily adapted to a wide range of pathogens and drugs, and highlight the benefits of drug nanoencapsulation towards safe controlled release.

Two new copper dimers, namely, bis(dimethyl sulfoxide)tetrakis(μ-pyrene-1-carboxylato)dicopper(Cu—Cu), [Cu2(C17H9O2)4(C2H6OS)2] or [Cu2(pyr-COO−)4(DMSO)2] (1), and bis(dimethylformamide)tetrakis(μ-pyrene-1-carboxylato)dicopper(Cu—Cu), [Cu2(C17H9O2)4(C3H7NO)2] or [Cu2(pyr-COO−)4(DMF)2] (2) (pyr = pyrene), were synthesized from the reaction of pyrene-1-carboxylic acid, copper(II) nitrate and triethylamine from solvents DMSO and DMF, respectively. While 1 crystallized in the space group P\overline{1}, the crystal structure of 2 is in space group P21/n. The Cu atoms have octahedral geometries, with four oxygen atoms from carboxylate pyrene ligands occupying the equatorial positions, a solvent molecule coordinating at one of the axial positions, and a Cu...Cu contact in the opposite position. The packing in the crystal structures exhibits π–π stacking interactions and short contacts through the solvent molecules. The Hirshfeld surfaces and two-dimensional fingerprint plots were generated for both compounds to better understand the intermolecular interactions and the contribution of heteroatoms from the solvent ligands to the crystal packing. In addition, a Cu2+/Cu1+ quasi-reversible redox process was identified for compound 2 using cyclic voltammetry that accounts for a diffusion-controlled electron-donation process to the Cu dimer.

Abstract The research proposed in this paper focuses on the analysis of perception for the selection of postgraduate programs in face-to-face and virtual modality in the Directorate of Postgraduate and Continuing Education of the ESPAM MFL. Although it is true, face-to-face education is of vital importance for the formation of a student, since it fixes their attention in place, time, and space, promoting social interaction that becomes a key to success in learning. However, in the last three years, in the face of adverse and unexpected situations, virtual university education has prepared to revolutionize the concept of learning beyond face-to-face learning. The objective of this research is inclined toward the description of the appreciation of students who have received master's classes in both virtual and face-to-face modalities. In order to obtain the data that allowed defining the results and conclusions, a survey was applied through a Google Forms questionnaire with a total of 13 questions that determined the perception that 87 students have when selecting a postgraduate program, and it raised a descriptive investigation to represent the data found. Finally, with the information obtained, it was possible to show that the applicants reflect a favorable attitude toward learning in virtual environments, because it allows them to continue with their other activities and foster skills with the use of technological tools that contribute to creativity, innovation, and arouse interest in learning.

Delineating spatial boundaries that accurately encompass complex, often cryptic, life histories of highly migratory marine megafauna can be a significant conservation challenge. For example, marine turtles range across vast ocean basins and coastal areas, thus complicating the evaluation of relative impacts of multiple overlapping threats and the creation of coherent conservation strategies. To address these challenges, spatially explicit ‘regional management units’ (RMUs) were developed in 2010 for all marine turtle species, globally. RMUs were intended to provide a consistent framework that organizes conspecific assemblages into units above the level of nesting rookeries and genetic stocks, but below the species level, within regional entities that may share demographic trajectories because they experience similar environmental conditions and other factors. From their initial conception, RMUs were intended to be periodically revised using new information about marine turtle distributions, life history, habitat use patterns, and population structure. Here, we describe the process used to update the 2010 RMU framework by incorporating newly published information and inputs from global marine turtle experts who are members of the IUCN Marine Turtle Specialist Group. A total of 48 RMUs for 6 of 7 marine turtle species and 166 distinct genetic stocks for all 7 species are presented herein. The updated RMU framework reflects a significant advance in knowledge of marine turtle biology and biogeography, and it provides improved clarity about the RMU concept and its potential applications. All RMU products have been made open access to support research and conservation initiatives worldwide.

Abstract Background Opioid use disorder (OUD) is a deadly illness that remains undertreated, despite effective pharmacological treatments. Barriers, such as stigma, treatment affordability, and a lack of training and prescribing within medical practices result in low access to treatment. Software-delivered measurement-based care (MBC) is one way to increase treatment access. MBC uses systematic patient symptom assessments to inform an algorithm to support clinicians at critical decision points. Method Focus groups of faculty clinicians (N = 33) from 3 clinics were conducted to understand perceptions of OUD diagnosis and treatment and whether a computerized MBC model might assist with diagnosis and treatment. Themes from the transcribed focus groups were identified in two phases: (1) content analysis focused on uncovering general themes; and (2) systematic coding and interpretation of the data. Results Analysis revealed six major themes utilized to develop the coding terms: “distinguishing between chronic pain and OUD,” “current practices with patients using prescribed or illicit opioids or other drugs,” “attitudes and mindsets about providing screening or treatment for OUD in your practice,” “perceived resources needed for treating OUD,” “primary care physician role in patient care not specific to OUD,” and “reactions to implementation of proposed clinical decision support tool.” Conclusion Results revealed that systemic and attitudinal barriers to screening, diagnosing, and treating OUD continue to persist. Providers tended to view the software-based MBC program favorably, indicating that it may be a solution to increasing accessibility to OUD treatment; however, further interventions to combat stigma would likely be needed prior to implementation of these programs. Trial Registration ClinicalTrials.gov; NCT04059016; 16 August 2019; retrospectively registered; https://clinicaltrials.gov/ct2/show/NCT04059016 .

Aerosol particles in Southeast Asia are challenging to characterize due to their complex life cycle within the diverse topography and weather of the region. An emerging aerosol climatology was established based on AErosol RObotic NETwork (AERONET) data (December 2009 to October 2018) for clear-sky days in Metro Manila, the Philippines. Aerosol optical depth (AOD) values were highest from August to October, partly from fine urban aerosol particles, including soot, coinciding with the burning season in insular Southeast Asia when smoke is often transported to Metro Manila during the southwest monsoon. Clustering of AERONET volume size distributions (VSDs) resulted in five aerosol particle sources based on the position and magnitude of their peaks in the VSD and the contributions of specific particle species to AOD per cluster based on MERRA-2. The clustering showed that the majority of aerosol particles above Metro Manila were from a clean marine source (58 %), which could be related to AOD values there being relatively low compared to other cities in the region. The following are the other particle sources over Metro Manila: fine polluted sources (20 %), mixed-dust sources (12 %), urban and industrial sources (5 %), and cloud processing sources (5 %). Furthermore, MERRA-2 AOD data over Southeast Asia were analyzed using empirical orthogonal functions. Along with AOD fractional compositional contributions and wind regimes, four dominant aerosol particle air masses emerged: two sulfate air masses from East Asia, an organic carbon source from Indonesia, and a sulfate source from the Philippines. Knowing the local and regional aerosol particle air masses that impact Metro Manila is useful in identifying the sources while gaining insight into how aerosol particles are affected by long-range transport and their impact on regional weather.

Abstract The indigenous population of the Canary Islands, which colonized the archipelago around the 3rd century CE, provides both a window into the past of North Africa and a unique model to explore the effects of insularity. We generate genome-wide data from 40 individuals from the seven islands, dated between the 3rd–16rd centuries CE. Along with components already present in Moroccan Neolithic populations, the Canarian natives show signatures related to Bronze Age expansions in Eurasia and trans-Saharan migrations. The lack of gene flow between islands and constant or decreasing effective population sizes suggest that populations were isolated. While some island populations maintained relatively high genetic diversity, with the only detected bottleneck coinciding with the colonization time, other islands with fewer natural resources show the effects of insularity and isolation. Finally, consistent genetic differentiation between eastern and western islands points to a more complex colonization process than previously thought.

The controlled-swap and controlled-controlled-not gates are at the heart of the original proposal of reversible classical computation by Fredkin and Toffoli. Their widespread use in quantum computation, both in the implementation of classical logic subroutines of quantum algorithms and in quantum schemes with no direct classical counterparts, has made it imperative early on to pursue their efficient decomposition in terms of the lower-level gate sets native to different physical platforms. Here, we add to this body of literature by providing several logically equivalent circuits for the Toffoli and Fredkin gates under all-to-all and linear qubit connectivity, the latter with two different routings for control and target qubits. Besides achieving the lowest cnot counts in the literature for all these configurations, we also demonstrate the remarkable effectiveness of the obtained decompositions at mitigating coherent errors on near-term quantum computers via equivalent circuit averaging. We first quantify the performance of the method in silico with a coherent-noise model before validating it experimentally on a superconducting quantum processor. In addition, we consider the case where the three qubits on which the Toffoli or Fredkin gates act nontrivially are not adjacent, proposing a novel scheme to reorder them that saves one cnot for every swap. This scheme also finds use in the shallow implementation of long-range cnots. Our results highlight the importance of considering different entangling gate structures and connectivity constraints when designing efficient quantum circuits.

Introduction: Mesenchymal stromal cells (MSC) are envisioned as a potential cellular vehicle for targeted cancer therapies due to their tumor tropism and immune permissiveness. An obstacle in their use is the duality in their interactions within tumors, rendering them pro-tumorigenic or anti-tumorigenic, in a context dependent manner. MSC preconditioning, or priming, has been proposed as a strategy for directing the effector properties of MSC at tumor sites.Methods: We primed human MSC derived from adipose tissues (ASC), a clinically advantageous MSC source, utilizing toll-like receptor agonists. Subsequently, we explored the consequences in tumor progression and transcriptome upon the interaction of tumor cells with primed or unprimed ASC in an in vivo model of prostate cancer, the second most common cancer and second leading cause of cancer related death in men in the USA.Results and discussion: In the studied model, poly I:C-primed ASC were found to significantly accelerate tumor growth progression. And while unprimed and LPS-primed ASC did not exert a significant effect on tumor growth at the macroscopic level, gene expression analyses suggested that all treatments promoted distinct modulatory effects in the tumor microenvironment, including altered modulation of angiogenesis, and immune response processes. However, the effects resulting from the collective interaction across these processes must be sufficiently skewed in a pro-tumorigenic or anti-tumorigenic direction for evidence of tumor progression modulation to be detectable at the macroscopic level. Our study highlights potential MSC-tumor microenvironment interactions that may be leveraged and should be considered in the development of cancer therapeutics utilizing MSC.

Introduction Metabolic syndrome (MS) is associated with abnormalities in atrial mechanics, atrial remodeling, and an increased risk of heart rhythm disorders. One of the most commonly used approaches to the prevention of cardiac remodeling in arterial hypertension is the administration of renin–angiotensin system (RAS) inhibitors. Therefore, this study aimed to investigate the effects of RAS inhibitors on atrial mechanics parameters in patients with MS. Methods and materials This longitudinal observational study included 55 patients with hypertension and MS, as defined by the ATP III criteria. The patients were evaluated at the start of antihypertensive treatment with an RAS inhibitor. The patients’ clinical characteristics, chosen pharmacological treatment, and transthoracic echocardiography findings were recorded at baseline and 6 months thereafter. A student's dependent sample t -test was used for comparisons between groups. Pearson correlation was used to evaluate the relationships between variables. Results Patients with MS had higher peak atrial longitudinal strain (PALS) values at 6 months than at baseline. Meanwhile, systolic strain and peak late strain rates were lower at follow-up than at baseline. The different antihypertensive treatments had comparable effects on the PALS changes during the follow-up period. Higher high-density lipoprotein levels at baseline were correlated with changes in PALS. Conclusion The administration of RAS inhibitors improved atrial mechanics parameters in the early stages of antihypertensive management in MS.

BackgroundDemographic changes in the world population have resulted in an increasingly aging society, with a progressive increase in the number of people in situations of dependence, who require assistance from family members to meet their basic needs. Caring for older adults involves performing diverse activities, resulting in reduced free time and tiredness, and fulfilling the demands and expectations related to personal, family, physical, and social life, consequently compromising the quality of life of the caregiver. In this context, the informal caregiver of hospitalized older adults emerges as the focus of attention. ObjectiveThe aim of this study was to describe the sociodemographic profile, health conditions, and burden of informal caregivers of older adults admitted to a university hospital in Brazil during the COVID-19 pandemic period. MethodsThis is a cross-sectional, descriptive, and analytical study that was conducted with 25 informal caregivers of hospitalized older adults in a university hospital in Brazil between August and September 2022. Three instruments were applied: Caregiver Burden Inventory, sociodemographic questionnaire, and health conditions questionnaire. The data were analyzed using SPSS version 28.0. Descriptive (frequency and percentage) and inferential analyses were performed using 2-sided Student t test with 95% CIs. ResultsOf the 25 interviewees, 18 (72%) were females, 17 (46%) were married or in a stable union, 14 (56%) completed secondary education, and 11 (44%) lived with the older adults who needed care. The average age of the participants was 44 (SD 12.8) years. Regarding their health conditions, most caregivers self-reported it as good (12/25, 48%). They provided care to their father or mother older than 70 years (14/25, 56%). The Caregiver Burden Inventory analysis showed that the caregivers were the most negatively impacted in the domains of personal life overload (mean 10.8, SD 3.46; P=.047) and physical overload (mean 10.6, SD 2.32; P=.02). ConclusionsIn recent years, there has been an increase in the burden on informal caregivers of hospitalized older adults in Brazil, thereby impacting their personal and physical lives. The findings of our study show that health care professionals should be trained to promote health guidelines and actions to improve the personal and physical lives of the caregiver population in Brazil.

Abstract Wind turbines are increasingly expanding worldwide and Doubly‐Fed Induction Generator (DFIG) is a key component of most of them. Stator winding fault is a major fault in this equipment and its incipient detection is of vital importance. However, there is a paucity of research in this field. In this study, a novel machine learning‐based method is proposed for incipient detection of inter‐turn short‐circuit fault (ITF) in the DFIG stator based on the current signals of the stator. The proposed method makes use of state‐of‐the‐art deep learning methods along with conventional signal processing tools and general machine learning techniques. More specifically, the incipient fault detection problem is regarded as a multi‐class classification problem and a Long Short‐Term Memory network, which is more appropriate for time‐series data is utilised for inference. Furthermore, a variant of the celebrated Empirical mode Decomposition analysis tool is used to extract some well‐known statistical features among which the most informative ones are selected using a new feature selection method. Our tests using experimental data in steady‐state conditions show that the proposed method can accurately detect ITF fault at its initial stage when only one turn is shorted. Moreover, its performance is considerably higher than that of a variety of machine learning‐based methods.