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Enceladus, an icy moon of Saturn, possesses an internal water ocean and jets expelling ocean material into space. Cassini investigations indicated that the subsurface ocean could be a habitable environment having a complex interaction with the rocky core. Further investigation of the composition of the plume formed by the jets is necessary to fully understand the ocean, its potential habitability, and what it tells us about Enceladus' origin. Moonraker has been proposed as an ESA M-class mission designed to orbit Saturn and perform multiple flybys of Enceladus, focusing on traversals of the plume. The proposed Moonraker mission consists of an ESA-provided platform, with strong heritage from JUICE and Mars Sample Return, and carrying a suite of instruments dedicated to plume and surface analysis. The nominal Moonraker mission has a duration of 13.5 years. It includes a 23-flyby segment with 189 days allocated for the science phase, and can be expanded with additional segments if resources allow. The mission concept consists in investigating: i) the habitability conditions of present-day Enceladus and its internal ocean, ii) the mechanisms at play for the communication between the internal ocean and the surface of the South Polar Terrain, and iii) the formation conditions of the moon. Moonraker, thanks to state-of-the-art instruments representing a significant improvement over Cassini's payload, would quantify the abundance of key species in the plume, isotopic ratios, and physical parameters of the plume and the surface. Such a mission would pave the way for a possible future landed mission., Accepted for publication in The Planetary Science Journal

The general-purpose PreAmplifier–DIscriminator application-specific integrated circuit (ASIC) chip, PADI, was originally designed to be used as front-end electronics (FEE) for reading out the timing resistive plate chambers in the time-of-flight (TOF) wall of the compressed baryonic matter (CBM) experiment of the future Facility for Antiproton and Ion Research (FAIR) facility in Darmstadt, Germany. Here, we present the last models of this 0.18- $\mu \text{m}$ CMOS technology—PADI-X, PADI-XI, and PADI-XII—as well as their key features and test results. While the PADI series was originally developed for high-energy physics experiments carried out at ground facilities, it turned out that PADI is also suitable for space experiments and PADI-X was selected for one sensor of the European Space Agency, JUpiter ICy moons Explore (JUICE) mission. Currently, the most recent model of the series, PADI-XII, has been tuned for space applications and the prototype batch is currently under production.

Energetic neutral atoms (ENAs) are produced by the neutralization of energetic ions formed by shock-accelerated gradual solar energetic particle events (SEP). These high-energy ENAs (HENAs) can reach the Earth earlier than the associated SEPs and provide information about the SEPs at the lower corona. The HENA properties observed at Earth depend on the properties of the coronal mass ejection (CME)-driven shocks that accelerate the SEPs. With a model of HENA production in a shock-accelerated SEP event, we semi-quantitatively investigate the energy-time spectrum of HENAs depending on the width, propagation speed, and direction of the shock, as well as the density and ion abundances of the lower corona. Compared to the baseline model parameters, the cases with a wider shock width angle or a higher coronal density would increase the HENA flux observed at the Earth, while the case with an Earth-propagating shock shows a softened HENA spectrum. The comparison of expected HENA fluxes in different cases with a flight-proven ENA instrument suggests that solar HENAs can feasibly be monitored with current technologies, which could provide a lead time of 2-3 hours for SEPs at a few MeV. We propose that monitoring of solar HENAs could provide a new method to forecast shock-driven SEP events that could have significant space weather impacts on the near-Earth environment.

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The ESA-JAXA BepiColombo mission to Mercury will provide simultaneous measurements from two spacecraft, offering an unprecedented opportunity to investigate magnetospheric and exospheric particle dynamics at Mercury as well as their interactions with solar wind, solar radiation, and interplanetary dust. The particle instrument suite SERENA (Search for Exospheric Refilling and Emitted Natural Abundances) is flying in space on-board the BepiColombo Mercury Planetary Orbiter (MPO) and is the only instrument for ion and neutral particle detection aboard the MPO. It comprises four independent sensors: ELENA for neutral particle flow detection, Strofio for neutral gas detection, PICAM for planetary ions observations, and MIPA, mostly for solar wind ion measurements. SERENA is managed by a System Control Unit located inside the ELENA box. In the present paper the scientific goals of this suite are described, and then the four units are detailed, as well as their major features and calibration results. Finally, the SERENA operational activities are shown during the orbital path around Mercury, with also some reference to the activities planned during the long cruise phase.

Lake Mead National Recreational Area (LMNRA) serves as critical habitat for several federally listed species and supplies water for municipal, domestic, and agricultural use in the Southwestern U.S. Contaminant sources and concentrations vary among the sub-basins within LMNRA. To investigate whether exposure to environmental contaminants is associated with alterations in male common carp (Cyprinus carpio) gamete quality and endocrine- and reproductive parameters, data were collected among sub-basins over 7 years (1999–2006). Endpoints included sperm quality parameters of motility, viability, mitochondrial membrane potential, count, morphology, and DNA fragmentation; plasma components were vitellogenin (VTG), 17s-estradiol, 11-keto-testosterone, triiodothyronine, and thyroxine. Fish condition factor, gonadosomatic index, and gonadal histology parameters were also measured. Diminished biomarker effects were noted in 2006, and sub-basin differences were indicated by the irregular occurrences of contaminants and by several associations between chemicals (e.g., polychlorinated biphenyls, hexachlorobenzene, galaxolide, and methyl triclosan) and biomarkers (e.g., plasma thyroxine, sperm motility and DNA fragmentation). By 2006, sex steroid hormone and VTG levels decreased with subsequent reduced endocrine disrupting effects. The sperm quality bioassays developed and applied with carp complemented endocrine and reproductive data, and can be adapted for use with other species.

The Advanced Small Analyzer for Neutrals (ASAN) on board the Chang’E-4 Yutu-2 rover first detected energetic neutral atoms (ENAs) originating from the lunar surface at various lunar local times on the lunar farside. In this work, we examine the ENA energy spectra, obtained in the first 23 lunar days from 2019 January 11 to 2020 October 12, and find a higher ENA differential flux on the lunar dawnside than on the duskside. Combined with Acceleration, Reconnection, Turbulence and Electrodynamics of the Moon’s Interaction with the Sun (ARTEMIS) data, we analyze the correlation between the ENA differential flux and solar wind parameters, such as flux, density, dynamic pressure, and velocity, for each ASAN energy channel on the dawnside and duskside. The results show that ENA differential flux is positively correlated with solar wind flux, density, and dynamic pressure and relatively lower on the duskside than on the dawnside. To determine the relationship between solar wind energy and ENA energy, we analyze the correlation between solar wind energy and ENA cutoff energy and temperature on the dawnside and duskside. The results show that the ENA cutoff energy and temperature are lower on the duskside than on the dawnside at the same solar wind energy. The difference between the ENA–solar wind observation on the dawnside and duskside is possibly caused by solar wind deflection and deceleration on the duskside, which can be attributed to the interaction between solar wind and the lunar magnetic anomalies located nearby in the northwestern direction of the Chang’E-4 landing site.

Objective Organosiloxanes are prevalent in personal care products (PCPs) due to the desired properties they impart in the usage and application of such products. However, the European Chemical Agency (ECHA) has recently published restriction proposals on the amount of two cyclic siloxanes, octamethylcyclotetrasiloxane (D4) and decamethylcyclotetrasiloxane (D5) allowed in wash off products such as shampoos and conditioners which are discharged down the drain during consumer use. This legislation will require that reliable analytical methods are available for manufacturers and government agencies to use in documenting compliance with the restrictions. This paper proposes a simple analytical method to enable accurate measurement of these compounds down to the circa 0.1 weight percent level in PCPs. Methods While gas chromatography methods are reported in the literature for quantitation of D4 and D5 in several matrices including PCPs, the potential for generation of false positives due to contamination, co-elution, and in-situ generation of cyclic volatile methylsiloxanes (cVMS) is always present and needs to be controlled. This report demonstrates the applicability of using a combination of emulsion break, liquid-liquid extraction and silylation sample preparation followed by GC-FID analysis as a suitable means of analyzing PCPs for specific cVMS. Results The reliability and limitations of such methodology was demonstrated through several round robin studies conducted in the laboratories of a consortium of silicone manufacturers. In addition, this report presents examples of false positives encountered during development of the method and presents a comparative analysis between this method and a published QuEChERS sample preparation procedure to illustrate the potential for generation of false positives when an inappropriate approach is applied to determination of cVMS in personal care products. Conclusion This report demonstrates that an approach to determine cVMS levels in personal care products is to perform an emulsion break on the sample, isolate the nonpolar phase from the emulsion break and treat with a silylation reagent to abate potential in situ formation of cyclics during the course of GC-FID analysis. Round robin studies conducted in laboratories representing multiple siloxane manufacturers demonstrated the reliability of the GC-FID method when measuring cVMS in PCPs down to circa 0.1%. This article is protected by copyright. All rights reserved.

Centrioles are critical for the formation of centrosomes, cilia and flagella in eukaryotes. They are thought to assemble around a nine-fold symmetric cartwheel structure established by SAS-6 proteins. Here, we have engineered Chlamydomonas reinhardtii SAS-6-based oligomers with symmetries ranging from five- to ten-fold. Expression of a SAS-6 mutant that forms six-fold symmetric cartwheel structures in vitro resulted in cartwheels and centrioles with eight- or nine-fold symmetries in vivo. In combination with Bld10 mutants that weaken cartwheel-microtubule interactions, this SAS-6 mutant produced six- to eight-fold symmetric cartwheels. Concurrently, the microtubule wall maintained eight- and nine-fold symmetries. Expressing SAS-6 with analogous mutations in human cells resulted in nine-fold symmetric centrioles that exhibited impaired length and organization. Together, our data suggest that the self-assembly properties of SAS-6 instruct cartwheel symmetry, and lead us to propose a model in which the cartwheel and the microtubule wall assemble in an interdependent manner to establish the native architecture of centrioles.

Botulinum neurotoxin A causes botulism but is also used for medical and cosmetic applications. A detailed molecular understanding of BoNT/A--host receptor interactions is therefore fundamental for improving current clinical applications and for developing new medical strategies targeting human disorders. Towards this end, we recently solved an X-ray crystal structure of BoNT/A1 in complex with its neuronal protein receptor SV2C. Based on our findings, we discuss the potential implications for BoNT/A function.

PLATO (PLAnetary Transits and Oscillation of stars) is the ESA Medium size dedicated to exo-planets discovery, adopted in the framework of the Cosmic Vision program. The PLATO launch is planned in 2026 and the mission will last at least 4 years in the Lagrangian point L2. The primary scientific goal of PLATO is to discover and characterize a large amount of exo-planets hosted by bright nearby stars, constraining with unprecedented precision their radii by mean of transits technique and the age of the stars through by asteroseismology. By coupling the radius information with the mass knowledge, provided by a dedicated ground-based spectroscopy radial velocity measurements campaign, it would be possible to determine the planet density. Ultimately, PLATO will deliver the largest samples ever of well characterized exo-planets, discriminating among their ‘zoology’. The large amount of required b right stars can be achieved by a relatively small aperture telescope (about 1 meter class) with a wide Field of View (about 1000 square degrees). The PLATO strategy is to split the collecting area into 24 identical 120 mm aperture diameter fully refractive cameras with partially overlapped Field of View delivering an overall instantaneous sky covered area of about 2232square degrees. The opto-mechanical sub-system of each camera, namely Telescope Optical Unit, is basically composed by a 6 lenses fully refractive optical system, presenting one aspheric surface on the front lens, and by a mechanical structure made in AlBeMet.

Guidelines have been published for improving management of chronic heart failure (CHF). We examined the association between improved guideline adherence and risk for all-cause death in patients with stable systolic HF.Data on ambulatory patients (2006-2010) with CHF and reduced ejection fraction (HF-REF) from the Austrian Heart Failure Registry (HIR Austria) were analysed. One-year clinical data and long-term follow-up data until all-cause death or data censoring were available for 1014 patients (age 65 [55-73], male 75%, NYHA class I 14%, NYHA II 56%, NYHA III/IV 30%). A guideline adherence indicator (GAI [0-100%]) was calculated for each patient at baseline and after 12 ± 3 months that considered indications and contraindications for ACE-I/ARB, beta blockers, and MRA. Patients were considered ΔGAI-positive if GAI improved to or remained at high levels (≥ 80%). ΔGAI50+ positivity was ascribed to patients achieving a dose of ≥ 50% of suggested target dose.Improvements in GAI and GAI50+ were associated with significant improvements in NYHA class and NT-proBNP (1728 [740-3636] to 970 [405-2348]) (p0.001). Improvements in GAI50+, but not GAI, were independently predictive of lower mortality risk (HR 0.55 [95% CI 0.34-0.87; p=0.01]) after adjustment for a large variety of baseline parameters and hospitalisation for heart failure during follow-up.Improvement in guideline adherence with particular emphasis on dose escalation is associated with a decrease in long-term mortality in ambulatory HF-REF subjects surviving one year after registration.

Kinesin spindle protein (KSP), an ATP-dependent motor protein, plays an essential role in bipolar spindle formation during the mitotic phase (M phase) of the normal cell cycle. KSP has emerged as a novel target for antimitotic anticancer drug development. In this work, we synthesized a range of new biphenyl compounds and investigated their properties in vitro as potential antimitotic agents targeting KSP expression. Antiproliferation (MTT (=3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide)) assays, combined with fluorescence-assisted cell sorting (FACS) and Western blot studies analyzing cell-cycle arrest confirmed the mechanism and potency of these biphenyl compounds in a range of human cancer cell lines. Structural variants revealed that functionalization of biphenyl compounds with bulky aliphatic or aromatic groups led to a loss of activity. However, replacement of the urea group with a thiourea led to an increase in antiproliferative activity in selected cell lines. Further studies using confocal fluorescence microscopy confirmed that the most potent biphenyl derivative identified thus far, compound 7, exerts its pharmacologic effect specifically in the M phase and induces monoaster formation. These studies confirm that chemical scope remains for improving the potency and treatment efficacy of antimitotic KSP inhibition in this class of biphenyl compounds.

Structural analysis of a complex of tubulin and tubulin tyrosine ligase (TTL) reveals insights into TTL’s enzymatic mechanism, how it discriminates between α- and β-tubulin, and its possible evolutionary origin., Tubulin tyrosine ligase (TTL) catalyzes the post-translational retyrosination of detyrosinated α-tubulin. Despite the indispensable role of TTL in cell and organism development, its molecular mechanism of action is poorly understood. By solving crystal structures of TTL in complex with tubulin, we here demonstrate that TTL binds to the α and β subunits of tubulin and recognizes the curved conformation of the dimer. Biochemical and cellular assays revealed that specific tubulin dimer recognition controls the activity of the enzyme, and as a consequence, neuronal development. The TTL–tubulin structure further illustrates how the enzyme binds the functionally crucial C-terminal tail sequence of α-tubulin and how this interaction catalyzes the tyrosination reaction. It also reveals how TTL discriminates between α- and β-tubulin, and between different post-translationally modified forms of α-tubulin. Together, our data suggest that TTL has specifically evolved to recognize and modify tubulin, thus highlighting a fundamental role of the evolutionary conserved tubulin tyrosination cycle in regulating the microtubule cytoskeleton.

Background An antimicrobial stewardship program (ASP), aiming to optimize antimicrobial usage, was implemented at a Veterans Affairs Health Care System (VA HCS). Objective The main objective of this study was to compare antimicrobial usage before and after implementation of an ASP and to assess ASP interventions. Method This retrospective study was conducted at the Fargo VA HCS. A total of 1,017 inpatient charts were reviewed for 2 distinct time periods, February through September 2008 (pre ASP) and February through September 2010 (post ASP). The data that were collected and analyzed included the number of hospitalized patients prescribed antimicrobials, antimicrobial therapy duration, duration of hospital stay, and inpatient antimicrobial costs. Subgroup analyses were performed on the top 5 antimicrobials and the top 6 indications. The number, types of, and overall acceptance rate of ASP interventions were also assessed. Results When the pre- to post-ASP periods were compared, the percentage of patients on antimicrobial therapy decreased from 36.8% to 25% ( P < .001), the median duration of antimicrobial therapy significantly decreased ( P = .02), and the defined daily dose (DDD) per 1,000 patient bed days was reduced for piperacillin/tazobactam, vancomycin, and ciprofloxacin. In addition, the total inpatient antimicrobial costs decreased by $48,044 (25%). The overall ASP intervention acceptance rate was 81.6% (315 out of 386 total interventions). Conclusion The results of this study show that ASP implementation has been highly accepted by providers and has been associated with a reduction in the number of patients prescribed antimicrobials, median duration of antimicrobial therapy, and antimicrobial inpatient costs.

A detailed molecular understanding of botulinum neurotoxin (BoNT)/host-cell-receptor interactions is fundamental both for developing strategies against botulism and for generating improved BoNT variants for medical applications. The X-ray crystal structure of the receptor-binding domain (HC) of BoNT/A1 in complex with the luminal domain (LD) of its neuronal receptor SV2C revealed only few specific side-chain – side-chain interactions that are important for binding. Notably, two BoNT/A1 residues, Arg 1156 and Arg 1294, that are crucial for the interaction with SV2, are not conserved among subtypes. Because it has been suggested that differential receptor binding of subtypes might explain their differences in biological activity, we determined the crystal structure of BoNT/A2-HC in complex with SV2C-LD. Although only few side-chain interactions are conserved between the two BoNT/A subtypes, the overall binding mode of subtypes A1 and A2 is virtually identical. In the BoNT/A2-HC – SV2C complex structure, a missing cation-π stacking is compensated for by an additional salt bridge and an anion-π stacking interaction, which explains why the binding of BoNT/A subtypes to SV2C tolerates variable side chains. These findings suggest that motif extensions and a shallow binding cleft in BoNT/A-HC contribute to binding specificity.

Thermal effects in PLATO are analyzed in terms of uniform temperature variations, longitudinal and lateral temperature gradients. We characterize these effects by evaluating the PSF centroid shifts and the Enclosed Energy variations across the whole FoV. These patterns can then be used to gauge the thermal behavior of each individual telescope in order to improve the local photometric calibration across the PLATO field of view.

PLATO stands for PLAnetary Transits and Oscillation of stars and is a Medium sized mission selected as M3 by the European Space Agency as part of the Cosmic Vision program. The strategy behind is to scrutinize a large fraction of the sky collecting lightcurves of a large number of stars and detecting transits of exo-planets whose apparent orbit allow for the transit to be visible from the Earth. Furthermore, as the transit is basically able to provide the ratio of the size of the transiting planet to the host star, the latter is being characterized by asteroseismology, allowing to provide accurate masses, radii and hence density of a large sample of extra solar bodies. In order to be able to then follow up from the ground via spectroscopy radial velocity measurements these candidates the search must be confined to rather bright stars. To comply with the statistical rate of the occurrence of such transits around these kind of stars one needs a telescope with a moderate aperture of the order of one meter but with a Field of View that is of the order of 50 degrees in diameter. This is achieved by splitting the optical aperture into a few dozens identical telescopes with partially overlapping Field of View to build up a mixed ensemble of differently covered area of the sky to comply with various classes of magnitude stars. The single telescopes are refractive optical systems with an internally located pupil defined by a CaF2 lens, and comprising an aspheric front lens and a strong field flattener optical element close to the detectors mosaic. In order to continuously monitor for a few years with the aim to detect planetary transits similar to an hypothetical twin of the Earth, with the same revolution period, the spacecraft is going to be operated while orbiting around the L2 Lagrangian point of the Earth-Sun system so that the Earth disk is no longer a constraints potentially interfering with such a wide field continuous uninterrupted survey.

The project PLAnetary Transits and Oscillations of stars (PLATO) is one of the selected medium class (M class) missions in the framework of the ESA Cosmic Vision 2015-2025 program. The mean scientific goal of PLATO is the discovery and study of extrasolar planetary systems by means of planetary transits detection. The opto mechanical subsystem of the payload is made of 32 normal telescope optical units (N-TOUs) and 2 fast telescope optical units (FTOUs). The optical configuration of each TOU is an all refractive design based on six properly optimized lenses. In the current baseline, in front of each TOU a Suprasil window is foreseen. The main purposes of the entrance window are to shield the following lenses from possible damaging high energy radiation and to mitigate the thermal gradient that the first optical element will experience during the launch from ground to space environment. In contrast, the presence of the window increases the overall mass by a non-negligible quantity. We describe here the radiation and thermal analysis and their impact on the quality and risks assessment, summarizing the trade-off process with pro and cons on having or dropping the entrance window in the optical train.

The project PLAnetary Transits and Oscillations of stars (PLATO) is one of the selected medium class (M class) missions in the framework of the ESA Cosmic Vision 2015-2025 program. The main scientific goal of PLATO is the discovery and study of extrasolar planetary systems by means of planetary transits detection. According to the current baseline, the scientific payload consists of 34 all refractive telescopes having small aperture (120mm) and wide field of view (diameter greater than 37 degrees) observing over 0.5-1 micron wavelength band. The telescopes are mounted on a common optical bench and are divided in four families of eight telescopes with an overlapping line-of-sight in order to maximize the science return. Remaining two telescopes will be dedicated to support on-board star-tracking system and will be specialized on two different photometric bands for science purposes. The performance requirement, adopted as merit function during the analysis, is specified as 90% enclosed energy contained in a square having size 2 pixels over the whole field of view with a depth of focus of +/-20 micron. Given the complexity of the system, we have followed a Montecarlo analysis approach for manufacturing and alignment tolerances. We will describe here the tolerance method and the preliminary results, speculating on the assumed risks and expected performances.

Die Arthroskopie des Ellenbogengelenkes gewinnt zunehmend an Bedeutung. Eine umfassende Untersuchung und praoperative Diagnostik sind fur eine korrekte Indikationsstellung entscheidend. Neben den haufigen Eingriffen an Gelenkhaut und Knorpel konnen bei zunehmender Expertise auch Instabilitaten sowie Bewegungseinschrankungen arthroskopisch therapiert werden. Eine Ausweitung der Indikationsstellung auf die Frakturversorgung des Ellenbogengelenkes stellt aktuell noch eine Ausnahme dar.

In den letzten Jahren gewannen die diagnostische und die operative Arthroskopie der Schulter zunehmend an Bedeutung. Die Arthroskopie ergibt im Vergleich zu anderen Diagnostikverfahren die zuverlassigsten und meisten Informationen bei Schultergelenklasionen. Vor allem die arthroskopische Versorgung von Weichteilverletzungen drangte die offenen Verfahren immer mehr in den Hintergrund. Labrumlasionen nach traumatischen Schulterluxationen, Lasionen der langen Bizepssehne, Rotatorenmanschettenlasionen und das subakromiale Impingementsyndrom sind Indikationen fur arthroskopische Operationsverfahren. Durch die Weiterentwicklung von Instrumenten und speziellen Ankersystemen werden die Operationstechniken immer effektiver und schneller und somit letztlich auch sicherer fur den Patienten.

Shooting range soils frequently contain anomalous concentrations of metals (e.g. Pb, Zn, Mn) and Sb coming from bullets which may be released into the environment. In a pot experiment, we investigated metal and Sb uptake by three plant species (Plantago lanceolata, Lolium perenne and Triticum aestivum) growing on a calcareous shooting range soil (pH 7.8; 500 mg kg−1 Pb, 21 mg kg−1 Sb) and the uptake changes when an acidic fertilizer solution was applied to the soil. Metal and Sb solubility in the soil was determined by extraction with 0.1 M NaNO3. In addition, we measured pH, electrical conductivity and dissolved organic carbon in drainage samples. The results showed significant increase over time of pH (from 7.8 to 8.3) and decrease of electrical conductivity and dissolved organic carbon (from 230 to ∼130 mg L−1). Fertilizer application increased NaNO3-extractable Pb and Sb and root:shoot biomass ratio but not plant metal uptake. In T. aestivum spikes accumulated more Zn, Ni and Cu than shoots and grains. Mn and Zb uptake was correlated in L. perenne shoots. P. lanceolata, a Sb-bioindicator, did not accumulate high amounts of Sb (

Contamination of shooting range soils with toxic trace elements, in particular Pb and Sb, is of increasing environmental concern worldwide. We studied the extractability of Sb, and other metals in two shooting range soils: a calcareous soil (pH 8) with low organic carbon (0.5%) and a non-calcareous soil (pH 6.3) with elevated organic carbon content (5%). Both soils contained total concentrations of around 500 mg kg(-1) Pb, 65 mg kg(-1) Cu, 100 mg kg(-1) Zn and 20 mg kg(-1) Sb. We tested the effects of Ca(OH)(2), phosphate and sodium humate amendments on metals and Sb extractability. Extracts with H(2)O and NaNO(3) contained 0.02-0.05% of the total Zn and Pb; 0.1-0.5% of total Ni and Cu and approximately 1% of total Sb. Sequential extraction procedure of Zeien and Brümmer resulted in similar percentages for the sum of the two most labile fractions (F1+F2) in two soils: 10% Pb, and 15-20% Sb. Water and NaNO(3)-extractable Sb concentrations increased after phosphate addition, but were not affected by the addition of sodium humate. The results show that leaching of Sb from shooting ranges into ground and surface waters may generate a serious environmental risk under widely different soils conditions.

Largemouth bass (Micropterus salmoides) and common carp (Cyprinus carpio) were collected from 13 sites located in the Mobile (MRB), Apalachicola–Flint–Chattahoochee (ARB), Savannah (SRB), and Pee Dee (PRB) River Basins to document spatial trends in accumulative chemical contaminants, health indicators, and reproductive biomarkers. Organochlorine residues, 2,3,7,8-tetrachlorodibenzo-p-dioxin-like activity (TCDD-EQ), and elemental contaminants were measured in composite samples of whole fish, grouped by species and gender, from each site. Mercury (Hg) and polychlorinated biphenyls (PCBs) were the primary contaminants of concern. Concentrations of Hg in bass samples from all basins exceeded toxicity thresholds for piscivorous mammals (> 0.1 μg/g ww), juvenile and adult fish (> 0.2 μg/g ww), and piscivorous birds (> 0.3 μg/g ww). Total PCB concentrations in samples from the MRB, ARB, and PRB were > 480 ng/g ww and may be a risk to piscivorous wildlife. Selenium concentrations also exceeded toxicity thresholds (> 0.75 μg/g ww) in MRB and ARB fish. Concentrations of other formerly used (total chlordanes, dieldrin, endrin, aldrin, mirex, and hexachlorobenzene) and currently used (pentachlorobenzene, pentachloroanisole, dacthal, endosulfan, γ-hexachlorocyclohexane, and methoxychlor) organochlorine residues were generally low or did not exceed toxicity thresholds for fish and piscivorous wildlife. TCDD-EQs exceeded wildlife dietary guidelines (> 5 pg/g ww) in MRB and PRB fish. Hepatic ethoxyresorufin O-deethylase (EROD) activity was generally greatest in MRB bass and carp. Altered fish health indicators and reproductive biomarker were noted in individual fish, but mean responses were similar among basins. The field necropsy and histopathological examination determined that MRB fish were generally in poorer health than those from the other basins, primarily due to parasitic infestations. Tumors were found in few fish (n = 5; 0.01%); ovarian tumors of smooth muscle origin were found in two ARB carp from the same site. Intersex gonads were identified in 47 male bass (42%) representing 12 sites and may indicate exposure to potential endocrine disrupting compounds. Comparatively high vitellogenin concentrations (> 0.35 mg/mL) in male fish from the MRB, SRB, and PRB indicate exposure to estrogenic or anti-androgenic chemicals.

The objective of this study was to evaluate the effects of bleached/unbleached kraft mill effluents (B/UKME) on the reproductive parameters of free-ranging Florida largemouth bass (Micropterus salmoides floridanus). The reproductive parameters measured included gonadosomatic index (GSI), histological evaluation of gonads, and plasma concentrations of vitellogenin (VTG), 17beta-estradiol, and 11-ketotestosterone (11-KT). Hepatic ethoxyresorufin-O-deethylase (EROD) activity was measured as a marker of exposure to cytochrome P450-inducing agents in these effluents. Endpoints were compared among adult bass sampled from tributary and mainstream effluent-contaminated and reference sites. Females sampled from the site closest to the mill outfall had a significant five-fold increase in EROD activity compared to bass sampled from reference streams. Although sex hormones were significantly reduced in bass from exposed sites, there were no differences in VTG and GSI across sites. The absence of organism-level responses was probably not related to a lack of sensitivity, as previous studies in our laboratory have shown that bass exposed to these effluents exhibit changes in GSI and in other measures associated with reproductive success. In females, inverse relationships were observed between VTG and GSI and EROD activity. These relationship, however, were not consistent within all of the sites studied. Collectively, our findings indicate that hepatic EROD induction is an effective marker of B/UKME exposure in largemouth bass and that it might be associated with antiestrogenic effects in this species.

Since Europe is considering to establish a “market-driven” European Global Navigation Satellite System, the German Center of Aerospace initiated a market research to justify a German investment in such a European project. The market research performed included the following market segments: aviation, railway, road traffic, shipping, surveying, farming, military, space applications, leisure, and sport. In these market segments, the forementioned inputs were determined for satellite navigation hardware (receivers) as well as satellite navigation services. The forecast period was from year 2007 to 2017. For the considered period, the market amounts to a total of DM 83.0 billion (approx. US $50 billion), whereas the satellite navigation equipment market makes up DM 39.8 billion, and charges for value-added-services amount to DM 43.2 billion. On closer examination road traffic can be identified as the dominant market share, both in the receiver-market and service-market. With a share of 96% for receivers and 73% for services the significance of the road traffic segment becomes obvious. The second part of this paper investigates the effects the market potential has on the Value-Adding-Chain. Therefore, all participants in the Value-Adding-Chain are identified, using industrial cost structure models the employment effect is analyzed, and possible tax revenues for the state are examined.