Search

The objective of this digestibility assessment was to determine whether there are significant differences in the digestibility of Roundup Ready (glyphosate-tolerant) and conventional sugar beet, fodder beet, and beet pulp produced from sugar beet varieties when fed to sheep (seven wethers per treatment group). Three experiments were conducted in this assessment. Experiment 1 (35 wethers) compared one glyphosate-tolerant fodder beet variety with four conventional varieties, Exp. 2 (42 wethers) compared one glyphosate-tolerant sugar beet variety with five conventional varieties, and Exp. 3 (42 wethers) compared beet pulp derived from glyphosate-tolerant sugar beet with beet pulp from five European locations. The experimental phase consisted of a 2-wk preliminary period followed by a 1-wk collection period for Exp. 1 and 2, and a 1-wk preliminary period followed by a 1-wk digestibility collection period for Exp. 3. Diets were comprised of grass hay at 30, 30, and 20% of DM for Exp. 1, 2, and 3, respectively, with the balance being beet components. Urea and sodium sulfate were supplemented (8 and 2.9 g, respectively, for Exp. 1 and 2; and 6 g and 2.16 g, respectively, for Exp. 3) to supply sufficient dietary N and S. Each diet was fed to sheep (96 +/- 0.9 kg) in the three experiments to at or near maintenance energy levels. Treatment differences were considered significant at P < 0.05. Apparent digestibilities of DM, OM, CP, NDF, ADF, and DE for glyphosate-tolerant fodder and sugar beets did not differ from those for commercial fodder and sugar beets in Exp. 1 and 2. There were differences (P < 0.05) in DM, OM, CP, NDF, ADF, and DE digestibilities influenced by the different varieties of beet pulp in Exp. 3, but these were not unique to just the Roundup Ready sugar beet variety. Digestibilities and feeding values of Roundup Ready fodder beet, sugar beet, and beet pulp produced from Roundup Ready sugar beet varieties were not influenced by the introduction of the Roundup Ready trait compared with conventional varieties.

The objective of this study was to investigate the effect of NDF content in highly digestible grass/clover silage on particle size reduction, ruminal stratification, and selective retention in dairy heifers. The reduction in particle size from feed to feces was evaluated and related to feed intake, chewing activity, and apparent digestibility. Four grass/clover harvests (Mixtures of Lolium perenne, Trifolium pratense, and Trifolium repens) were performed from early May to late August at different maturities, at different regrowth stages, and with different clover proportions, resulting in silages with NDF contents of 312, 360, 371, and 446 g/kg DM, respectively, and decreasing NDF digestibility with greater NDF content. Four rumen-fistulated dairy heifers were fed silage at 90% of ad libitum level as the only feed source in a 4 × 4 Latin square design. Silage, ingested feed boluses, medial and ventral ruminal digesta, and feces samples were washed with neutral detergent in nylon bags of 10-μm pore size, freeze dried, and divided into small (0.212 mm), medium (0.212 to 1 mm), and large : LP;1 mm) particles by dry-sieving. Chewing activity, rumen pool size, and apparent digestibility were measured. Intake of NDF increased linearly from 2.3 to 2.8 kg/d with greater NDF content of forages (P = 0.01), but silages were exposed to similar eating time (P = 0.55) and rumination time per kg NDF (P = 0.35). No linear effect of NDF content was found on proportion of LP in ingested feed boluses (P = 0.31), medial rumen digesta (P = 0.95), ventral rumen digesta (P = 0.84), and feces (P = 0.09). Greater proportions of DM (P0.001) and particulate DM (P = 0.008) were found in medial ruminal digesta compared with ventral rumen, and differences in DM proportion increased with greater NDF content (P = 0.02). Particle size distributions were similar for digesta from the medial and ventral rumen regardless of NDF content of the silages (P0.13). The LP proportion was30% of particles in the ventral and medial rumen, whereas in the feces, the LP proportion was2%. Particle size stratification of the rumen was undetectable regardless of NDF content of the silages, stressing that the retention mechanism of large undigested particles lies elsewhere than with particle entrapment in the rumen mat. In this study, forage particle breakdown, ruminal stratification, and retention of particles in the rumen were not affected by NDF content of highly digestible grass/clover silages.

Expressing the protein value of a food involves measurements of several of its characteristics. Many in vivo studies have shown, that the protein degradability in the rumen varies substantially both between and within foods and therefore estimation of protein degradability in the rumen is an important task in protein evaluation. The most common method used has been the in situ (in sacco, nylon bag) method but many in vitro methods have been introduced and are based on use of either buffer solubility, chemical methods, rumen fluid or enzymes. None of these in vitro methods has proven to be of general use. In further development of in vitro methods as well as the in situ method a major problem is lack of a set of samples with a ‘true’ in vivo degradability which can be used for calibration of alternative methods. Microbial protein synthesis in the rumen has to be related to food characteristics which can be analysed easily. In vitro methods which can predict organic matter digestibility in foods are available and can be used to predict microbial protein synthesis in the rumen. Intestinal digestibility of undegraded dietary protein varies substantially both between and within foods and easy methods to estimate intestinal digestibility are therefore essential. The mobile bag method is easy to use and seems to give reliable results on most foods but requires access to duodenal cannulated animals which prevents this method from being routine. Alternative in vitro methods have been developed but further research is required for validation of these methods on a wide range of foods before they can be accepted for general use.

This study was conducted with the objective to validate a rumination monitoring system (RMS). The RMS was developed by SCR Engineers Ltd. (Netanya, Israel) to record individual rumination times in commercial dairy herds. Four heifers were fed 4 forage types at 3 feeding levels in 4 periods in a Latin square design. The forage types were spring growth of grass/clover harvested early (May 9) and late (May 25) conserved as silage and hay, creating 4 forage types: early harvest silage (ES), early harvest hay (EH), late harvest silage (LS), and late harvest hay (LH). Feeding levels were ad libitum, 90% of ad libitum, and 60% of ad libitum. Data were collected for 24 h at ad libitum feeding level, for 96 h at 90% of ad libitum, and for 24 h at 60% of ad libitum. Rumination time was recorded by the RMS in minutes per 2-h interval (RT(RMS2_i)) and per 24-h interval (RT(RMS24)). As a reference method, rumination time was recorded by chewing halters identifying rumination time by jaw movements (JM) and was accumulated into minutes per 2-h interval (RT(JM2_i)) and per 24-h interval (RT(JM24)) over the same time intervals as RT(RMS2_i) and RT(RMS24). Differences between RT(RMS2_i) and RT(JM2_i) were observed for EH, LS, and LH, with differences of 3.4 (P0.001), 1.1 (P = 0.03), and 3.3 (P0.001) min per 2-h interval. In addition, feeding restrictively at 90% and 60% of ad libitum resulted in greater rumination time recorded by the RMS compared to JM, with differences of 2.6 min per 2-h interval (P0.001) for 90% of ad libitum and 1.6 min per 2-h interval (P = 0.01) for 60% of ad libitum. Further, the differences between rumination times recorded by the RMS compared to JM were different among the 4 heifers. Despite these effects, rumination time recorded by the RMS correlated well with rumination time recorded by JM with correlation between RT(RMS2_i) and RT(JM2_i) of r = 0.91 (P0.001) and between RT(RMS24) and RT(JM24) of r = 0.79 (P0.001).

The objective of this digestibility assessment was to determine whether there are significant differences in the digestibility of Roundup Ready (glyphosate-tolerant) and conventional sugar beet, fodder beet, and beet pulp produced from sugar beet varieties when fed to sheep (seven wethers per treatment group). Three experiments were conducted in this assessment. Experiment 1 (35 wethers) compared one glyphosate-tolerant fodder beet variety with four conventional varieties, Exp. 2 (42 wethers) compared one glyphosate-tolerant sugar beet variety with five conventional varieties, and Exp. 3 (42 wethers) compared beet pulp derived from glyphosate-tolerant sugar beet with beet pulp from five European locations. The experimental phase consisted of a 2-wk preliminary period followed by a 1-wk collection period for Exp. 1 and 2, and a 1-wk preliminary period followed by a 1-wk digestibility collection period for Exp. 3. Diets were comprised of grass hay at 30, 30, and 20% of DM for Exp. 1, 2, and 3, respectively, with the balance being beet components. Urea and sodium sulfate were supplemented (8 and 2.9 g, respectively, for Exp. 1 and 2; and 6 g and 2.16 g, respectively, for Exp. 3) to supply sufficient dietary N and S. Each diet was fed to sheep (96 +/- 0.9 kg) in the three experiments to at or near maintenance energy levels. Treatment differences were considered significant at P0.05. Apparent digestibilities of DM, OM, CP, NDF, ADF, and DE for glyphosate-tolerant fodder and sugar beets did not differ from those for commercial fodder and sugar beets in Exp. 1 and 2. There were differences (P0.05) in DM, OM, CP, NDF, ADF, and DE digestibilities influenced by the different varieties of beet pulp in Exp. 3, but these were not unique to just the Roundup Ready sugar beet variety. Digestibilities and feeding values of Roundup Ready fodder beet, sugar beet, and beet pulp produced from Roundup Ready sugar beet varieties were not influenced by the introduction of the Roundup Ready trait compared with conventional varieties.