1. Performance of a multiplex polymerase chain reaction panel for identifying bacterial pathogens causing pneumonia in critically ill patients with COVID-19
- Author
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Thibaut Poncin, Béatrice Berçot, Mourad Benyamina, Alexandre Mebazaa, François Caméléna, Jérôme Le Goff, Benoit Plaud, Emmanuel Dudoignon, Anne Clotilde Moy, Lucie Guillemet, Matthieu Lafaurie, Maxime Coutrot, Maïté Chaussard, Mélissa Budoo, François Dépret, Benjamin Deniau, Infection, Anti-microbiens, Modélisation, Evolution (IAME (UMR_S_1137 / U1137)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Université Sorbonne Paris Nord, Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Marqueurs cardiovasculaires en situation de stress (MASCOT (UMR_S_942 / U942)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Université Sorbonne Paris Nord, French-Clinical Research Infrastructure Network - F-CRIN [Paris] (Cardiovascular & Renal Clinical Trialists - CRCT ), CCSD, Accord Elsevier, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP)-Université Sorbonne Paris Nord, and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Université Sorbonne Paris Nord
- Subjects
Male ,0301 basic medicine ,Microbiology (medical) ,Time Factors ,Microbiological culture ,Critical Illness ,[SDV]Life Sciences [q-bio] ,030106 microbiology ,medicine.disease_cause ,Sensitivity and Specificity ,Article ,Microbiology ,03 medical and health sciences ,0302 clinical medicine ,Multiplex polymerase chain reaction ,Pneumonia, Bacterial ,medicine ,Humans ,030212 general & internal medicine ,Pathogen ,Aged ,Bacteriological Techniques ,Bacteria ,medicine.diagnostic_test ,Pseudomonas aeruginosa ,business.industry ,SARS-CoV-2 ,Bacterial pneumonia ,COVID-19 ,General Medicine ,Rapid diagnostics ,Middle Aged ,medicine.disease ,3. Good health ,[SDV] Life Sciences [q-bio] ,Pneumonia ,Infectious Diseases ,Bronchoalveolar lavage ,Staphylococcus aureus ,Female ,business ,Bronchoalveolar Lavage Fluid ,Multiplex Polymerase Chain Reaction ,FilmArray Pneumonia Plus - Abstract
International audience; The FilmArray® Pneumonia Plus (FA-PP) panel can provide rapid identifications and semiquantitative results for many pathogens. We performed a prospective single-center study in 43 critically ill patients with coronavirus disease 2019 (COVID-19) in which we performed 96 FA-PP tests and cultures of blind bronchoalveolar lavage (BBAL). FA-PP detected 1 or more pathogens in 32% (31/96 of samples), whereas culture methods detected at least 1 pathogen in 35% (34/96 of samples). The most prevalent bacteria detected were Pseudomonas aeruginosa (n = 14) and Staphylococcus aureus (n = 11) on both FA-PP and culture. The FA-PP results from BBAL in critically ill patients with COVID-19 were consistent with bacterial culture findings for bacteria present in the FA-PP panel, showing sensitivity, specificity, and positive and negative predictive value of 95%, 99%, 82%, and 100%, respectively. Median turnaround time for FA-PP was 5.5 h, which was significantly shorter than for standard culture (26 h) and antimicrobial susceptibility testing results (57 h).
- Published
- 2021