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During mining activities, deep-sea mining vehicles (DSMVs) are highly susceptible to causing massive disturbance to the seafloor sediment, resulting in the formation of plumes due to underlying turbulence and currents. To gain a better understanding of the dispersion mechanism of sediment plumes, both experimental and numerical methods were employed. The numerical model was primarily used to characterize the solidity and liquidity of the sediment plume through volume of fluid and discrete phase model methods, respectively. The experimental data were validated against the numerical results. The plume distribution was studied in physical experiments for three different DSMV parameters. The study findings indicate that the discharge of the plume in the near field occurs in three stages due to a combination of plume release inertial forces, negative buoyancy in the water column, and wall restoring forces. Additionally, the increase in the travel velocity of the DSMV reduces the propagation of the plume in the direction of discharge and instead increases its lateral spread across the bottom surface. As the size of the DSMV decreases in three dimensions, changes in the vertical vortex structure become dominant in the plume distribution. This leads to a reduction in the length of the plume head and a faster sinking of the plume. When the wake Froude number Frw is between 0.7 and 6.8, representing the wake turbulence effect of DSMV on plume discharge, the diffusion width of the plume on the bottom surface is linearly related to Frw. [ABSTRACT FROM AUTHOR]

Aurovertins are the structurally diverse polyketides that distribute widely in different fungal species. They feature a 2,6-dioxabicyclo[3.2.1]-octane ring in structure and exhibit the potential antitumor activity against breast cancer as F1-ATPase β subunit inhibitor. In this study, we constructed the biosynthetic pathway of aurovertin in an Aspergillus nidulans host and obtained seven aurovertin-type compounds. Surprisingly, three new aurovertin geometric isomers were characterized. By introducing an inducible promoter xylP(p) in the pathway gene acyltransferase aurG, we can control the product ratios among different aurovertin compounds by adding glucose and/or inducer xylose. The yields of aurovertins could be increased up to about 20 times by adding a constitutive promoter gpdA(p) to transcription factor AurF, which indicates AurF's positive role in the biosynthesis of aurovertin. Taken together, our results provided not only an efficient way to generate bioactive fungal natural products but also realized the rational controlling their yields with designed promoters. [ABSTRACT FROM AUTHOR]