1. ANALYSIS OF THE BIOLOGICAL ACTIVITY OF ANTILYMPHOCYTE SERUM
- Author
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Perper, R J, Lyster, S C, Monovich, R E, and Bowersox, B E
- Abstract
Sequential serum samples were obtained from horses immunized two different methods of immunization using purified populations rat thymocytes or splenic lymphocytes. In vitro antibody titers were determined, and the ability of each sample to prolong skin graft survival, inhibit acute inflammation, or induce acute toxicity was studied. Although the number of horses immunized was small, the following preliminary conclusions were indicated. (1) The lymphocytotoxicity titers of the sera more closely paralleled the antiinflammatory activity than the immunosuppressive potency. (2) The acute toxic effect of unabsorbed serum correlated with the hemagglutination titer; however, after absorption with erythrocytes, the acute toxicity was independent of the level of hemagglutination, lymphocytotoxicity, and antiinflammatory or immunosuppressive potency. The histological lesions indicated a separate antibody population responsible for this activity, which was induced only by the serum obtained late in immunization from the animal immunized with spleen lymphocytes and not by the serum obtained from the three animals immunized with thymocytes with or without adjuvant. (3) Sera obtained early after both methods of immunization with either antigen were immunosuppressive. The immunosuppressive potency of serum induced with Freund's adjuvant did not decrease with booster injections; however, that induced without adjuvant eventually was lost, as were all other biological activities.
- Published
- 2023