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24 results on '"Lysson, Mariola"'

7. Mechanical strain and TLR4 synergistically induce cell-specific inflammatory gene expression in intestinal smooth muscle cells and peritoneal macrophages

Author

Wehner, Sven, Buchholz, Bettina M., Schuchtrup, Silke, Rocke, Anatol, Schaefer, Nico, Lysson, Mariola, Hirner, Andreas, and Kalff, Joerg C.

Subjects
Gene expression -- Physiological aspects, Smooth muscle -- Genetic aspects, Muscle cells -- Genetic aspects, Macrophages -- Genetic aspects, Biological sciences
Abstract

]?>Mechanical trauma of the gut is an unavoidable event in abdominal surgery. Former studies demonstrated that intestinal manipulation induces a strong inflammation within the tunica muscularis. We hypothesized that mechanical strain initiates or aggravates proinflammatory responses in intestinal smooth muscle cells (iSMC) or macrophages. First, an appropriate isolation and culture method for neonatal rat iSMC was established. Purified iSMC and primary peritoneal macrophages (pMacs) were subjected to static or cyclic strain, and gene expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), IL-6, and IL-1[beta] was analyzed by quantitative PCR. Supernatants from stretched iSMC were transferred to untreated pMacs or contrariwise, and medium transfer-triggered inflammatory gene expression was measured in unstretched cells. Finally, we investigated the synergistic effect of static strain on LPS-induced proinflammatory gene expression. Although cyclic strain failed, static strain significantly induced iNOS, COX-2, and IL-1[beta] mRNA in iSMC. pMacs showed an increase in all inflammatory genes investigated as well as macrophage inflammatory protein (MIP)-1[alpha] and MIP-2 mRNA after static strain. Both cell entities liberated unknown mediators in response to stretch that mutually stimulated iNOS gene expression. Finally, mechanostimulation amplified LPS-induced iNOS and IL-1[beta] gene expression in iSMC as well as COX-2 and IL-6 mRNA in pMacs. In conclusion, static strain initiates proinflammatory gene expression in iSMC and pMacs and triggers a bidirectional paracrine communication between both cultured cell entities via the liberation of unknown mediators. Furthermore, static strain synergistically operates with Toll-like receptor 4 ligation in a cell-specific manner. Hence, this study demonstrates that mechanical strain functions as an immunomodulatory stimulus in abdominal cells. postoperative inflammation; Toll-like receptor doi: 10.1152/ajpgi.00452.2009.

Published
2010

8. Inhibition of macrophage function prevents intestinal inflammation and postoperative ileus in rodents

Author

Wehner, Sven, Behrendt, Florian F., Lyutenski, Boris N., Lysson, Mariola, Bauer, Anthony J., Hirner, Andreas, and Kalff, Jorg C.

Subjects
Macrophages -- Research, Macrophages -- Health aspects, Rats -- Health aspects, Rats -- Research, Rattus -- Health aspects, Rattus -- Research, Colorectal diseases -- Research, Colorectal diseases -- Health aspects, Colorectal diseases -- Prevention, Gastrointestinal diseases -- Research, Gastrointestinal diseases -- Health aspects, Gastrointestinal diseases -- Prevention, Immunohistochemistry -- Evaluation, Health
Published
2007

11. A Population of Radio-Resistant Macrophages in the Deep Myenteric Plexus Contributes to Postoperative Ileus Via Toll-Like Receptor 3 Signaling

Author

Enderes, Jana, primary, Mallesh, Shilpashree, additional, Schneider, Reiner, additional, Hupa, Kristof J., additional, Lysson, Mariola, additional, Schneiker, Bianca, additional, Händler, Kristian, additional, Schlotmann, Balthasar, additional, Günther, Patrick, additional, Schultze, Joachim L., additional, Kalff, Jörg C., additional, and Wehner, Sven, additional

Published
2021
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12. A novel P2X2‐dependent purinergic mechanism of enteric gliosis in intestinal inflammation

Author

Schneider, Reiner, primary, Leven, Patrick, additional, Glowka, Tim, additional, Kuzmanov, Ivan, additional, Lysson, Mariola, additional, Schneiker, Bianca, additional, Miesen, Anna, additional, Baqi, Younis, additional, Spanier, Claudia, additional, Grants, Iveta, additional, Mazzotta, Elvio, additional, Villalobos‐Hernandez, Egina, additional, Kalff, Jörg C, additional, Müller, Christa E, additional, Christofi, Fedias L, additional, and Wehner, Sven, additional

Published
2020
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24. A role for 12/15‐lipoxygenase‐derived proresolving mediators in postoperative ileus: protectin DX‐regulated neutrophil extravasation

Author

Stein, Kathy, Stoffels, Melissa, Lysson, Mariola, Schneiker, Bianca, Dewald, Oliver, Krönke, Gerhard, Kalff, Jörg C., and Wehner, Sven

Abstract

Resolution of POI involves monocytotic 12/15‐LOX activity and generation of PDX, resulting in reduction of neutrophil infiltration into the postoperative bowel wall. Resolution of inflammation is an active counter‐regulatory mechanism involving polyunsaturated fatty acid‐derived proresolving lipid mediators. Postoperative intestinal motility disturbances, clinically known as postoperative ileus, occur frequently after abdominal surgery and are mediated by a complex inflammation of the intestinal muscularis externa. Herein, we tested the hypothesis that proresolving lipid mediators are involved in the resolution of postoperative ileus. In a standardized experimental model of postoperative ileus, we detected strong expression of 12/15‐lipoxygenase within the postoperative muscularis externa of C57BL/6 mice, predominately located within CX3CR1+/Ly6C+infiltrating monocytes rather than Ly6G+neutrophils. Mass spectrometry analyses demonstrated that a 12/15‐lipoxygenase increase was accompanied by production of docosahexaenoic acid‐derived lipid mediators, particularly protectin DX and resolvin D2, and their common precursor 17‐hydroxy docosahexaenoic acid. Perioperative administration of protectin DX, but not resolvin D2 diminished blood‐derived leukocyte infiltration into the surgically manipulated muscularis externa and improved the gastrointestinal motility. Flow cytometry analyses showed impaired Ly6G+/Ly6C+neutrophil extravasation after protectin DX treatment, whereas Ly6G‐/Ly6C+monocyte numbers were not affected. 12/15‐lipoxygenase‐deficient mice, lacking endogenous protectin DX synthesis, demonstrated increased postoperative leukocyte levels. Preoperative intravenous administration of a docosahexaenoic acid‐rich lipid emulsion reduced postoperative leukocyte infiltration in wild‐type mice but failed in 12/15‐lipoxygenase‐deficient mice mice. Protectin DX application reduced leukocyte influx and rescued 12/15‐lipoxygenase‐deficient mice mice from postoperative ileus. In conclusion, our results show that 12/15‐lipoxygenase mediates postoperative ileus resolution via production of proresolving docosahexaenoic acid‐derived protectin DX. Perioperative, parenteral protectin DX or docosahexaenoic acid supplementation, as well as modulation of the 12/15‐lipoxygenase pathway, may be instrumental in prevention of postoperative ileus.

Published
2016
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