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6 results on '"Lyons, Blair M."'

1. Integrated intracellular organization and its variations in human iPS cells

Author

Viana, Matheus P., Chen, Jianxu, Knijnenburg, Theo A., Vasan, Ritvik, Yan, Calysta, Arakaki, Joy E., Bailey, Matte, Berry, Ben, Borensztejn, Antoine, Brown, Eva M., Carlson, Sara, Cass, Julie A., Chaudhuri, Basudev, Cordes Metzler, Kimberly R., Coston, Mackenzie E., Crabtree, Zach J., Davidson, Steve, DeLizo, Colette M., Dhaka, Shailja, Dinh, Stephanie Q., Do, Thao P., Domingus, Justin, Donovan-Maiye, Rory M., Ferrante, Alexandra J., Foster, Tyler J., Frick, Christopher L., Fujioka, Griffin, Fuqua, Margaret A., Gehring, Jamie L., Gerbin, Kaytlyn A., Grancharova, Tanya, Gregor, Benjamin W., Harrylock, Lisa J., Haupt, Amanda, Hendershott, Melissa C., Hookway, Caroline, Horwitz, Alan R., Hughes, H. Christopher, Isaac, Eric J., Johnson, Gregory R., Kim, Brian, Leonard, Andrew N., Leung, Winnie W., Lucas, Jordan J., Ludmann, Susan A., Lyons, Blair M., Malik, Haseeb, McGregor, Ryan, Medrash, Gabe E., Meharry, Sean L., Mitcham, Kevin, Mueller, Irina A., Murphy-Stevens, Timothy L., Nath, Aditya, Nelson, Angelique M., Oluoch, Sandra A., Paleologu, Luana, Popiel, T. Alexander, Riel-Mehan, Megan M., Roberts, Brock, Schaefbauer, Lisa M., Schwarzl, Magdalena, Sherman, Jamie, Slaton, Sylvain, Sluzewski, M. Filip, Smith, Jacqueline E., Sul, Youngmee, Swain-Bowden, Madison J., Tang, W. Joyce, Thirstrup, Derek J., Toloudis, Daniel M., Tucker, Andrew P., Valencia, Veronica, Wiegraebe, Winfried, Wijeratna, Thushara, Yang, Ruian, Zaunbrecher, Rebecca J., Labitigan, Ramon Lorenzo D., Sanborn, Adrian L., Johnson, Graham T., Gunawardane, Ruwanthi N., Gaudreault, Nathalie, Theriot, Julie A., and Rafelski, Susanne M.

Published
2023
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3. Targeted Development of Registries of Biological Parts

Author

Peccoud, Jean, Blauvelt, Megan F., Cai, Yizhi, Cooper, Kristal L., Crasta, Oswald, DeLalla, Emily C., Evans, Clive, Folkerts, Otto, Lyons, Blair M., Mane, Shrinivasrao P., Shelton, Rebecca, Sweede, Matthew A., Waldon, Sally A., Peccoud, Jean, Blauvelt, Megan F., Cai, Yizhi, Cooper, Kristal L., Crasta, Oswald, DeLalla, Emily C., Evans, Clive, Folkerts, Otto, Lyons, Blair M., Mane, Shrinivasrao P., Shelton, Rebecca, Sweede, Matthew A., and Waldon, Sally A.

Abstract

The design and construction of novel biological systems by combining basic building blocks represents a dominant paradigm in synthetic biology. Creating and maintaining a database of these building blocks is a way to streamline the fabrication of complex constructs. The Registry of Standard Biological Parts (Registry) is the most advanced implementation of this idea. Methods/Principal Findings: By analyzing inclusion relationships between the sequences of the Registry entries, we build a network that can be related to the Registry abstraction hierarchy. The distribution of entry reuse and complexity was extracted from this network. The collection of clones associated with the database entries was also analyzed. The plasmid inserts were amplified and sequenced. The sequences of 162 inserts could be confirmed experimentally but unexpected discrepancies have also been identified. Conclusions/Significance: Organizational guidelines are proposed to help design and manage this new type of scientific resources. In particular, it appears necessary to compare the cost of ensuring the integrity of database entries and associated biological samples with their value to the users. The initial strategy that permits including any combination of parts irrespective of its potential value leads to an exponential and economically unsustainable growth that may be detrimental to the quality and long-term value of the resource to its users.

Published
2008
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4. TheArabidopsis thaliana Myo-Inositol 1-Phosphate Synthase1 Gene Is Required forMyo-inositol Synthesis and Suppression of Cell Death

Author

Donahue, Janet L., primary, Alford, Shannon R., additional, Torabinejad, Javad, additional, Kerwin, Rachel E., additional, Nourbakhsh, Aida, additional, Ray, W. Keith, additional, Hernick, Marcy, additional, Huang, Xinyi, additional, Lyons, Blair M., additional, Hein, Pyae P., additional, and Gillaspy, Glenda E., additional

Published
2010
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5. Targeted Development of Registries of Biological Parts

Author

Peccoud, Jean, primary, Blauvelt, Megan F., additional, Cai, Yizhi, additional, Cooper, Kristal L., additional, Crasta, Oswald, additional, DeLalla, Emily C., additional, Evans, Clive, additional, Folkerts, Otto, additional, Lyons, Blair M., additional, Mane, Shrinivasrao P., additional, Shelton, Rebecca, additional, Sweede, Matthew A., additional, and Waldon, Sally A., additional

Published
2008
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6. Arabidopsis thaliana Myo-Inositol 1-Phosphate Synthase1 Gene Is Required for Myo-inositol Synthesis and Suppression of Cell Death.

Author

Donahue, Janet L., Alford, Shannon R., Torabinejad, Javad, Kerwin, Rachel E., Nourbakhsh, Aida, Ray, W. Keith, Hernick, Marcy, Huang, Xinyi, Lyons, Blair M., Hein, Pyae P., and Gillaspy, Glenda E.

Subjects
CELL death, ARABIDOPSIS thaliana, VITAMIN C, FLUORESCENT proteins, SEED development
Abstract

l - myo- inositol 1-phosphate synthase (MIPS; EC 5.5.1.4) catalyzes the rate-limiting step in the synthesis of myo- inositol, a critical compound in the cell. Plants contain multiple MIPS genes, which encode highly similar enzymes. We characterized the expression patterns of the three MIPS genes in Arabidopsis thaliana and found that MIPS1 is expressed in most cell types and developmental stages, while MIPS2 and MIPS3 are mainly restricted to vascular or related tissues. MIPS1 , but not MIPS2 or MIPS3 , is required for seed development, for physiological responses to salt and abscisic acid, and to suppress cell death. Specifically, a loss in MIPS1 resulted in smaller plants with curly leaves and spontaneous production of lesions. The mips1 mutants have lower myo- inositol, ascorbic acid, and phosphatidylinositol levels, while basal levels of inositol (1,4,5)P3 are not altered in mips1 mutants. Furthermore, mips1 mutants exhibited elevated levels of ceramides, sphingolipid precursors associated with cell death, and were complemented by a MIPS1 -green fluorescent protein (GFP) fusion construct. MIPS1- , MIPS2- , and MIPS3-GFP each localized to the cytoplasm. Thus, MIPS1 has a significant impact on myo -inositol levels that is critical for maintaining levels of ascorbic acid, phosphatidylinositol, and ceramides that regulate growth, development, and cell death. [ABSTRACT FROM AUTHOR]

Published
2010
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