Search

Tomato (Solanum Lycopersicum L. (or) Lycopersicon esculentum Mill.) is being a very nutritious and health protective food, are highly perishable nature. Its sensitivity to postharvest loss due to poor handling, diseases and physical injury limits its successful marketing. Therefore, simple technology is required to reduce the postharvest loss of this commodity. The use of edible coatings with bio-extracts appears to be a good alternative preservation technique to extend the mature tomato fruits. This study was, therefore, initiated to investigate the effect of using bio- extracts garlic bulba and capsicum incorporation with coating materials (maize starch and beeswax on physicochemical quality of tomato fruit stored at ambient conditions (temperature 15.5 to 20.2oC and relative humidity of 55.5 to 67.3%). The experiment was conducted using complete randomized design of two varieties (Fetane and Melkashola) and six treatments. The tomato fruits were coated by dipping into solution for 3 minutes. The treatments prepared were on coating solution of MGE( 9.5% maize starch with 0.5% garlic extract), MCE (9.5% maize starch with 0.5% capsicum extract), BCE (9.5% beewax mixed with 0.5% capsicum extract), BGE (9.5% beewax mixed with 0.5% garlic extract), 10% maize starch without bio-extract, 10% beewax without bio- extract and control. The treatment means were tested at significance level of P ≤ 0.05. The effectiveness of bio-extracts with coating materials on physicochemical quality of tomato fruits were evaluated at three days intervals for 30 days. There was a significance difference (P

Field experiments were carried out during the 2015/16 and 2016/17 dry cropping seasons at the Experiment Farm of the Faculty of Agriculture, University of Benin, Benin City. The study was a 2 x 4 factorial fitted into a randomized complete block design (RCBD) in three replications. Two tomato varieties (Roma savannah and UC82) and four times of fertilizer application (T1: all fertilizer applied at 2 weeks after transplanting (2WAT), T2: 1/2 of the fertilizer applied at 2WAT and 1/2 at flowering, T3: one third 1/3 applied at 2WAT, 1/3 at flowering, 1/3 at fruiting and T4: all fertilizer applied at flowering) were used. In both years, stem diameter, number of flowers, fruits and fruit weight/plant (kg/ha) and yield were significantly improved by Roma savannah. Fruit diameter (5.70 cm and 4.75 cm) increased with UC82 compared with Roma savannah which produced the lowest fruit diameter (3.95 cm and 3.11 cm). Roma savannah had a higher fruit yield of 8.59 t ha-1 and 7.24 t ha-1 compared with 5.99 t ha-1 and 4.57 t ha-1 produced by UC82. In 2016/17 cropping season T3 and T4 significantly improved stem diameter above other treatments and T2 and T4 produced highest fruit yields which were similar. However, in both years highest fruit yields (10.39 t ha-1 and 7.09 t ha-1) were consistent with T2. The use of Roman savannah variety and T2 treatment should be encouraged for tomato production, in terms of higher fruit yield in this locality.

The reaction of 636 Solanum (sections Lycopersicon and Juglandifolia) accessions were evaluated under greenhouse conditions after mechanical inoculation with a Jordanian isolate of the new tobamovirus tomato brown rugose fruit virus (ToBRFV). Local and systemic infections were assayed by symptoms evaluation and virus detection via biotests and RT-PCR. All cultivated tomatoes (Solanum lycopersicum) and the great majority of wild tomato accessions proved susceptible to ToBRFV. They showed a wide range of symptoms (mosaic, leaf deformations, mottling, shoestring, and stunting). Twenty-six accessions representing S. lycopersicum var. cerasiforme, S. pimpinellifolium, S. habrochaites, and S. chilense were tolerant. High levels of resistance have been demonstrated in three accessions of S. ochrantum, a close relative to wild tomatoes (member of the sect. Juglandifolia) not only to ToBRFV but also to the tobamoviruses, tobacco mosaic virus (TMV) and tomato mosaic virus (ToMV). After mechanical inoculation, the three tobamoviruses could be detected only in inoculated leaves in the accessions LA2160, LA2162, and LA 2166, which remained symptomless. However, two other S. ochrantum accessions PI 473,498 and PI 230,519 reacted unusually. They were demonstrated highly resistant to TMV and ToMV, but proved transiently susceptible to ToBRFV showing mild systemic mosaic followed by total recovery from symptoms and the virus.

Aims: Research of alternative solutions to the use of synthetic pesticides. Study Design : Randomized block. Place and Duration of Study: Jean Lorougnon Guédé University (Daloa, Côte d’Ivoire), January to February 2020. Methodology: Extracts of Alchornea cordifolia, Tithonia diversifolia and Mezoneuron benthamianum were evaluated in vitro and in the nursery at 5 g/l, 15 g/l and 25 g/l. In vitro, the blotting paper method was used on tomato seed germination. In the nursery, the powdery extracts were added to the nursery soil bags with four treatments (5 g/l, 15 g/l, 25 g/l and control) and six bags per treatment with five tomato seeds per bag. Germination rate, number of leaves, height and the health status of the plants as well as the incidence and severity of root galls were evaluated. The results showed that in vitro the control had the highest average germination rate (98.5%) followed by T. diversifolia (43.03 to 98.5%), A. cordifolia (35.33 to 68.67%) and M. benthamianum (37.66 to 67.55%). However, in the nursery, T. diversifolial recorded the highest average treated seed germination (38 to 78.33%) and seedling emergence (12.78 to 38.33%) rates compared to the other treatments. Concerning growth parameters, the number of leaves (5.38 to 13.21) and height (5.71 to 15.64 cm) of tomato plants were influenced by the extracts type and concentration. The incidence of root galls was low for treated plants (0-60%) compared to controls (100%). The severity of the galls was significantly reduced by the extracts, however the extracts of T. diversifolia were the most effective. Extracts were more effective at low concentrations in improving agromorphological parameters of tomato, in contrast to the incidence and severity of galls which were reduced at high concentrations. Results suggest that these locally available plants could be used as biofertilizers and control method against root-knot nematodes.

The seriousness and menace of the worldwide weight of ‘Alzheimer’s disease have been related to a few factors, which incorporate antioxidant system depletion, mutation of proteins, and high expression of cholinesterases due to aging, environmental influence, diet, infectious agents, and hormonal imbalance. Overexpression of cholinesterases has been emphatically connected to ‘Alzheimer’s disease because of the unreasonable hydrolysis of acetylcholine and butyrylcholine. Certain plant phytochemicals, for example, beta-carotenoids, lutein, neoxanthin, and viola-xanthine from Lycopersicon esculentum Mill. Var. esculentum (ESC) and Lycopersicon esculentum Mill. Var. cerasiforme (CER) has been utilized altogether as a therapeutic candidate for the treatment of ‘Alzheimer’s disease. Therefore, this research sought to investigate the drug-likeness of the individual carotenoids as detailed for cholinesterase inhibition in the treatment of ‘Alzheimer’s disease. Four potential cholinesterase inhibitors from ESC and CER were retrieved from the PubChem database. Investigation of their drug-likeness, toxicity prediction, molecular docking, and dynamic simulations were carried out using Molinspiration, PreADMET V.2.0, Patchdock server, and Schrodinger Maestro software respectively. Neoxanthin was ranked the safest with a greater tendency to inhibit the cholinesterases with high binding affinity. In addition, its stability after simulation in a mimicked biological environment suggests its relevance as a potential drug candidate for the treatment of ‘Alzheimer’s disease through the inhibition of cholinesterases.

Bacterial spot caused by Xanthomonas euvesicatoria pv. euvesicatoria (Xanteu) is one of the major causes of yield losses in tomato (Lycopersicon esculentum (L.) Karsten ex Farw.) crops. The disease is mainly controlled by the use of cupric salts. The increasing demand for a more sustainable agriculture is urging the authorities to pass laws reducing the amounts of copper allowed. Therefore, the need to find new active antimicrobial principles is a priority, since costs, environmental damage and risks of selection of resistant bacterial strains are to be contained. The aim of the work was to investigate the potential antimicrobial properties of coumarin against Xanteu in order to define new sustainable control strategies which could lead to a significant reduction of copper. Results have shown that the use of half of the current copper hydroxide field dose and an innovative solution containing half of the current copper hydroxide field dose and 1% w/v coumarin makes it possible to diminish the bacterial population of Xanteu over time, while reducing the incidence and severity of in-planta disease.

Increasing demand for fresh tomato across the globe has necessitated imperative mechanism such as packaging materials for storage to extend shelf-life. In this study, shelf-life extension of tomato was studied using three packaging materials under two temperatures. The results with much emphasis on statistical interpretation revealed that the non-perforated high-density polyethylene (N-PHD) was effective in reducing deterioration of physicochemical, phytochemical and Colour composition of tomato. The second polynomial model predicted accurately the decomposition of weight loss, moisture content and firmness of tomato using statistical parameters. Colour was credited to variations in b* and a* parameters as an outcome of antioxidants, phenolics and total soluble solid disintegrations resulting from enzymatic activities. The results revealed that N-PHD packaging material combined with lower temperature could be a promising mechanism of storing tomato.

The study was conducted to analyse the comparative economic analysis and profitability of tomato production in selected districts of Punjab state. A primary data was collected in the year 2019-20 by using multistage stratified random sampling technique. Two top ranking districts namely Amritsar and Patiala on the basis of highest area under tomato crop were purposively selected in the Punjab state. A sample of 200 tomato growers were selected, 100 each from both the districts from 21 producing villages selected from Patiala and Amritsar district of Punjab state. The results of the study indicated that the average area under tomato crop was highest in case of Amritsar district i.e. 10.90 acres as compared to 2.98 acres in Patiala district. Majority of the farmers in both the districts were using hybrid varities. 575 variety is basically demanded by the factories or tomato companies and mostly preferred by processing firms. The total variable cost was estimated highest in case of Patiala district (Rs.59262.63/acre) as compared to Amritsar district (Rs.47779.15/acre) due to the difference in their cultivation methods (Bamboo staking vs open field cultivation), varietal difference, nursery preparation methods, seasonal and geographical difference. The returns over variable cost was found higher in case of Patiala district i.e. Rs. 85142.42 than Amritsar district (Rs.61882.74). The study concluded that the tomato cultivation in Patiala district was found more profitable than Amritsar district. It was recommended that the primary agricultural credit cooperative societies and other funding agencies should be persuaded to provide adequate short term credit facilities to cover the higher operational cost. Government should ensure the supply of hybrid seeds to tomato growers at subsidized rates and ensure better minimum support price to tomato growers so that farmers received price at least cost equal to the cost of production.

A lack of nutrients in the seed can be supplemented by seed coating and has the potential to improve germination and seedling growth. In this research, we studied the physiological effect of coating tomato seeds with plant nutrient formulations (PNF) on the dehydrogenase activity during germination and nutrient content of the seedlings. Two tomato seed lots were used, differing in physiological quality: the low quality seed lot had 76% germination and the medium quality seed lot had 87% germination. Seeds were coated with a complete PNF containing N, P, K, Ca, Mg, Fe, B, Mn, Cu and Zn. The PNF was applied at three concentrations, i.e., 1-fold (PF1), 4-fold (PF4) and 16-fold (PF16), and applied with 3% PVP-K90 + 1% PEG 6000 as a polymer. Coating seeds with PNF improved seed germination for both low- and medium-quality seeds. Accelerated ageing tests revealed that PF1 increased germination by 12 and 27% in low- and medium-quality seeds, respectively. All concentrations of PNF increased TDH activity after 48 hours of seed imbibition. Moreover, PF16 decreased root length but increased shoot length. All PNF treatments resulted in higher nutrient content in the seedlings.

Aims: This study evaluated the role of sodium, potassium, proline and soluble sugars accumulation in the ameliorative effect of an exogenous application of calcium on the detrimental effect of salinity on tomato plants. Study Design: The experiment was implemented as a Completely Randomized Design (RCD) with four treatments and three replications. Place and Duration of Study: The experiment was realized in a green house of the Faculty of Agronomical Sciences, University of Abomey-Calavi, Benin Republic from June to July 2020. Methodology: Three weeks old plants of the tomato cultivar “Padma” was submitted in pots to four treatments comprising the control (without NaCl); salt stress (120 mM NaCl) and a combination of 120 mM NaCl and exogenous application of 40 mM CaSO4 or Ca (NO3)2 by irrigation every two days. Plants growth, sodium (Na) and potassium (K) as well as proline and soluble sugars contents of leaves and roots were determined after two weeks. Results: Salt effect reduced significantly plant growth at P =.05, root K content at P =.05 and leaf K/Na ratio at P =.01 whereas it increased significantly leaf Na at P =.05 and root soluble sugars content at P =.05. The application of exogenous calcium (Ca) induced a significant amelioration of plant growth at P =.01 more marked with CaSO4 than with Ca (NO3)2. This treatment induced a significant decrease at P =.01 in leaf and root Na content and a significant increase at P =.05 in root K content only for CaSO4, a significant increase at P =.01 in leaf K/Na ratio and a significant increase at P =.01 in leaf and root proline content only for Ca(NO3)2. Conclusion: The ameliorative effect of both forms of calcium was due mainly to sodium (Na) exclusion from leaves and potassium (K) accumulation associated to a maintain of high K/Na ratio mainly in leaves. The importance of proline accumulation as an indicator of this ameliorative effect was associated only to Ca(NO3)2.

Genetic diversity is getting have been increasingly narrowed by the dense bred cultivar usage for production, on the other hand, genetic variation created by the landraces is known as the raw material of plant breeding. A collection of 21-landrace and three-commercial tomato (Lycopersicon esculentum Mill.) genotypes were screened for allele profile, genetic definition and genetic similarity with the aim of identifying genetic variability in genetic level with 40 SSR loci. Number of alleles per locus ranged from 3 (SSR50, SSR80, SSR9, LEtat002) to 13 (LEat018). Expected and observed heterozygosity values were scored between He: 0.753 and Ho: 0.714 respectively and the mean value of polymorphism information content (PIC) value of these loci was found as 0.694. Considering distribution of alleles at loci, the highest allele frequency was observed from SSR75 with % 62.5 LEat014 with % 58.3 LEga004 with % 50.0 locus, while the lowest was in AI491065 with 16.6% LEta024 locus. UPGMA (Unweighted Pair-Group Method using Arithmetic Means) method was conducted for cluster analysis. The dendrogram was consisted 2 main groups; Group 2 was the largest and contained many sub-groups. The highest genetic similarity level of genotypes from Bafra-Merkez and Sivas-Yukarıkale Village was found as 0.776. The lowest similarity ratio was observed between Sivas-Yıldızeli-Emirler Village and Samsun-Carsamba genotypes with 0.136. The obtained results are indicated that genetic diversity information of landraces with effective SSR loci will help to manage tomato genetic resources for tomato breeding.

This study aimed to investigate levels of copper in three commonly edible fruits, namely, Lycopersicon esculenta (tomato), Citrullus lanatus (watermelon) and Cucumis sativus (cucumber) traded in Dar es Salaam city. Also, the study scrutinized risk associated with heavy metal intake in the fruits. The fruits were collected from Buguruni Market, one of the biggest markets in the city, and thereafter, were processed, and analyzed to determine copper levels. The average daily intake and human health risk indices were calculated based on the obtained copper levels. The findings revealed that the mean levels and average daily intake in L. esculenta (0.3267 mg kg-1, 0.0279 mg kg-1 person-1) and C. lanatus (0.2523 mg kg-1, 0.0216 mg kg-1 person-1) did not differ considerably (p = 0.05) from each other while the two fruits had significantly higher values than C. sativus (0.1610 mg kg-1, 0.0137 mg kg-1 person-1). The copper levels and average daily intake values were below WHO/FAO and Tanzania Bureau of Standards permissible limits. The human health index (HRI) was in the order: L. esculenta > C. lanatus > C. sativus and all the values were less than unit, suggesting that there is no health risk from consuming the fruits. Concerning copper levels, the study concludes that the fruits are safe for human consumption.

Traditional tomato varieties are of particular importance for vegetable cultivation, by capitalizing on the diversity of local populations and increasing the number of crops. The study of local populations of cultivated tomatoes determines the accumulation of additional knowledge on how the shape, size and taste of fruits evolve, helping to identify new varieties with a role in improving the productivity, quality and nutritional value of tomatoes. In this paper, 2 local varieties from the Cluj County area were studied, from the point of view of the dynamics of certain chemical components when, after harvest, the tomatoes are stored at different stages of maturity. The lowest water loss, of 7.5%, was presented by the pink variety, stored for 7 days at a temperature of 8 °C. Vitamin C was better preserved in the case of the red variety, presenting after 7 days of storage at 8 °C, values of 27.23% at green maturity and 46.58% at consumption maturity. The pectic substances, in the case of the studied varieties, do not show significant variations depending on the maturity phase or the storage method, these registering values between 0.11 and 0.27%.

The present investigation “Studies on effects of graded levels of zinc and Trichoderma viride, Pseudomonas strita on yield and quality attributing characters in tomato (Lycopersicon esculentum L.)”. The experiment was laid out in Factorial RBD with two factor i.e. factor first is zinc solubilizers and factor second is levels of zinc, it has twelve treatments replicated three times. Trichoderma viride and Pseudomonas strita are used as source zinc solubilizers along with different levels of zinc. The experiment framed was intended to study the effect of zinc solubilizers and levels of zinc on yield and quality of tomato.The results revealed that effect of zinc solubilizers on yield and quality parameters of tomato indicated that the zinc solubilizer B2 (Trichoderma viride) recorded maximum fruit yield per plant (1458.76 gm), fruit yield per plot (20.42 kg), fruit yield per hectare (567.15 qt.), maximum titrable acidity (0.38 %), TSS (5.22%), ascorbic acid (22.81 mg/100gm), reducing sugars (1.61 %), non reducing sugars (2.70 %), totalsugars (4.31 %). The effect of different levels of zinc on yield and quality parameters of tomato indicated that the levels of zinc Zn3 (30 kg ZnSO4/ha) recorded maximum fruit yield per plant (1305.09 gm), fruit yield per plot (18.26 kg), fruit yield per hectare (507.43 qt.), maximum titrable acidity (0.36 %), TSS (4.77%), ascorbic acid (22.04 mg/100gm), reducing sugars (1.48 %), non reducing sugars (2.61 %), total sugars (4.09 %).The interaction effect of different zinc solubilizers and levels of zinc on yield and quality parameters of tomato indicated that the B2Zn3 (Trichoderma viride + 30 kg ZnSO4/ha) recorded maximum fruit yield per plant (1698.33 gm), fruit yield per plot (23.77 kg), fruit yield per hectare (660.27 qt.), maximum titrable acidity (0.43 %), TSS (5.39%),ascorbic acid (23.35 mg/100gm), reducing sugars (1.71 %), non reducing sugars (2.80 %), total sugars (4.51 %).

Uno de los principales problemas fitosanitarios en la producción de tomate o jitomate (Solanum lycopersicon Mill.) donde se utiliza el suelo como medio de cultivo es Fusarium spp., el control químico es el método frecuentemente utilizado para su manejo; con impactos económicos y ambientales importantes. Los objetivos del presente estudio fueron: evaluar el repollo como cultivo de rotación y la incorporación de los residuos al suelo sobre la incidencia de la marchitez permanente en plantas de tomate e incidencia de Fusarium spp., en el suelo y evaluar la supresión de Fusarium spp., mediante la aplicación de subproductos de hojas y tallos de repollo. El trabajo se realizó en dos etapas: en la primera se documentó la evolución de Fusarium spp., en un cultivo comercial de tomate, establecido bajo condiciones de malla antiáfidos y repollo como cultivo de rotación, durante el periodo de 2012 a 2019. En la segunda etapa, se evaluó un extracto acuoso y un polvo deshidratado, obtenidos de hojas y tallos residuales de repollo. Los resultados muestran que el repollo utilizado como cultivo de rotación y la incorporación al suelo de las hojas y tallos residuales, redujeron la incidencia de marchitez permanente en el cultivo de tomate y suprimieron eficazmente Fusarium spp. Asimismo, el polvo deshidratado obtenido de estos residuos mostró un efecto similar sobre el control de Fusarium spp., al obtenido en los tratamientos con metilditiocarbamato de sodio.

La tomate est l’un des légumes les plus consommés au monde et au Bénin. Il est largement établi que la salinité agit négativement sur la germination et la croissance et modifie considérablement la concentration des composés bioactives des légumes. Au Bénin, la tomate est cultivée partiellement sur les terres cultivables des régions côtières où la salinité du sol et de l’eau d’irrigation est une réalité. Cette étude visait à évaluer l’effet de la salinité sur la floraison, la fructification et la composition nutritionnelle des fruits de tomate du cultivar local Akikon. Les plantes de trois semaines ont été arrosées jusqu’à la maturité des fruits avec diverses concentrations de NaCl de 0, 30, 60 et 120 mM. La date d’apparition des premières fleurs, la date de maturation des fruits ainsi que les teneurs en protéines des fruits n’ont pas été significativement affectées par la salinité alors que le nombre de fruits par plante, le poids des fruits, les teneurs en sucres solubles totaux et en sucres réducteurs subissent une réduction significative sous l’effet du NaCl. Par contre, les teneurs en lycopène, en vitamines B6, B12 et C ont connu une augmentation significative avec l’augmentation de la concentration de NaCl. La salinité a donc réduit la production de tomate et amélioré la qualité nutritionnelle des fruits en augmentant notamment ses teneurs en vitamines et en lycopène.Mots clés: Salinité, teneur en nutriments, sucres, lycopène, vitamines. English title:Effect of salt stress on flowering, fructification and fruit nutritional quality of Benin local tomato (Lycopersicon esculentum Mill.) cultivar AkikonTomato (Lycopersicon esculentum) is one of the most important consumable vegetable in the world and in Benin. It is widely established that salinity acts negatively on germination and growth and considerably modifies the concentration of bioactive compounds in vegetables. In Benin, tomato is partially cultivated on arable lands in coastal regions where soil and irrigation water salinity is a reality. This study investigated the impact of salinity on flowering, fructification, and fruit nutrient contents of a local cultivar of tomato Akikon. Three weeks old plants were irrigated with four NaCl concentrations: 0; 30; 60 and 120 mM NaCl till fruit ripening. The date of first flower appearance, the date of fruit ripening and fruit protein content were not affected by salinity; whereas fruit number, fruit fresh mass, fruit soluble sugars and reducing sugars contents were significantly decreased by increasing salinity. Moreover, lycopen and vitamins B6, B12 and C contents increased significantly when salinity concentration increased. Thus, salinity reduced tomato productivity but improved fruit nutritional quality by mainly increasing vitamins and lycopen contents.Keywords: Salinity, nutrients content, lycopen, sugars, vitamins.

Salinity adversely affects 20-30% of the irrigated area in the world. Tomato is sensitive to salinity. It is one of the most severe abiotic factors of many agricultural crops and it becoming the main problem in Ethiopia. This study was conducted to evaluate the effects of different salinity levels on the seed germination parameters of tomato varieties. It was laid out in a completely randomized design with three replicates. The treatment included four tomato varieties (Sirinka, Weyno, ARP D2, and Roma VF) and five salinity levels (1 dS m-1, 2 dS m-1, 3 dS m-1, 4 dS m-1, and control). Fifty seeds were placed in a Petri dish over a moistened germination paper for germination and seedlings and allowed to grow for 14 days. The germination rate, speed and energy of tomato seeds were significantly (p < 0.001) affected by the combined effect of variety and salinity. The shortest mean germination time, the highest mean germination rate, and the highest speed of germination were recorded in the ARP D2 variety in the control treatment. The lowest first and last days of germination, and the uncertainty of germination were recorded from ARP D2. However, an increase in the days of germination and in the uncertainty of germination, and a decrease in the germination index and total germination percentage trends were observed with increasing salinity levels. The highest level of salinity (4 dS m-1) affected the germination of tomato varieties. Among the four tested tomato varieties, ARP D2 and Roma VF were tolerant to salinity.

This study was carried out to determine the extensity, development of population and the state of damage of tomato leaf miner (Tuta absoluta (Meyrick, 1917) (Lepidoptera: Gelechiidae)) in total eight tomato fields located in the villages of Goktepe of Central Sanliurfa, Meteler of Birecik, Kepirce of Bozova, Yalcinkaya of Ceylanpinar, Ovacik of Hilvan, Kucuk Yucelen of Siverek, Aligor of Suruc and Ciftciler of Viransehir in 2011. To determine the extensity of tomato leaf miner each of the 5 districts in the village tomato field was checked by observation whether adult or larvae found in fields T. absoluta. The result showed that tomato leaf miner was common in all the districts. The highest density of tomato leaf miner adult population quantities were 630, 800, 400, 965, 211, 600, 215 and 96 pcs/week in Goktepe of Central Sanliurfa, Meteler of Birecik, Kepirce of Bozova, Yalcinkaya of Ceylanpinar, Ovacik of Hilvan, Kucuk Yucelen of Siverek, Aligor of Suruc and Ciftciler of Viransehir, respectively. Tomato leaf miner harmed the fruits, particularly total in 1st and 2nd harvest of tomato (the end of August- the beginning of September) , if precautions were not taken. Moreover, it was recorded that if the adult population was quite dense in the fields study conducted, there aren’t any undamaged leaves and fruits from T. absoluta.

This experiment was conducted at the Faculty of Agriculture, AL-Azhar University, Nasr City, Cairo, Egypt during the two winter seasons of 2018-2019 and 2019-2020 to study the effect of saline levels in the diluted agricultural drainage water of 1000, 2000, 3000, 4000 ppm and tap water as a control (300 ppm) and foliar seaweed extract concentrations of 400, 600 , 800 ppm and tap water as a control and their interaction on the growth, yield and fruits quality of tomato (Lycopersicon esculentum L.) Magy hybrid under plastic house conditions. The obtained results showed that plant height, leaf area, fruit fresh weight, size, length and diameter, leaf chlorophyll A, B and carotenoids contents, in addition to total yield as fruit number and weight significantly increased with using the saline level of 1000 ppm and the foliar seaweed extract concentration of 800 ppm. On contrast, fruit contents of T.S.S and dry matter significantly increased with increasing the saline water levels up to 4000 ppm and the seaweed extract concentrations at 800 ppm. Also, the data indicated that fruit content of ascorbic acid significantly increased from using the 2000 ppm level saline water and the concentration of 800 ppm seaweed extract. In this respect, it could be concluded that the application of the seaweed extract concentration at 800 ppm was the most effective to decrease the adverse effect of saline irrigation water on the growth and yield of tomato plants.

Comparative study about the salt-induced oxidative stress and lipid peroxidation has been realised in primary root tissues for Tomato (Lycopersicon esculantum L.) in order to evaluate their responses to salt stress. Salinity impacts in terms of root growth, H2O2 generation, lipid peroxidation and membrane destabilisation were more pronounced in roots. Salt treatment in form of NaCl was given to the roots of the tomato plants in hydroponics culture. Root length was measured by centimetre scale, H2O2 and lipid peroxidation was confirmed by spectrophotometer. Absorbance for H2O2 estimation was recorded at 480 nm whereas for Lipid peroxidation was done at 600nm. When the tomato plants were treated with different concentrations of NaCl, it was observed that as the concentration of NaCl was increasing, there was decreased root growth resulting in reduced root length and proportionate increase in the amount of H2O2 production level with increase in the concentrations of NaCl treatment upto 300mM Concentration and Significant increase in Lipid peroxidation was observed with the increase in NaCl concentrations upto 500mM Concentration. Comparative response may be helpful in developing a better understanding of tolerance mechanisms to salt stress in Tomato.

The Effect of Sodium Azize and Grafting on Vegetative Growth PreliminaYield Parameters on Tomato in Wet Season, was investigated with the aim of inducing variability that could be exploited in the improvement of some quality traits in Tomato. The seeds of three varieties of tomato: (Roma, UC-82B and Local varieties) were treated with three different concentrations of sodium azide (0.2mM, 0.4 mM, 0.6 mM and 0.0 mM as control). The result showed highly significant difference (P≤0.01) in the effects of different concentrations of sodium azide on almost all the selected traits except on the fruit diameter where no significant difference was found. More so, the result also indicated highly significant difference (P≤0.01) among the varieties on the selected traits except in germination percent (1 WAP); where significant difference (P≤0.05) was found, and in number of leaves, and fruits diameters, no significant difference exists among the varieties. Furthermore, highly significant difference (P≤0.01) was found on the effects of grafting and sodium azide singly and in combine dosage as treatments on germination percent (1 and 2 WAP), and on number of leaves/plant. Significant difference (P≤0.05) was found on the effects of these treatments on the survival rate, while, no significant difference was found on the effects of the treatments on the number of fruits, fruit diameter, and pH. More so, no significant differences was found in the interactions except in the interaction in the interactions of variety and treatments on the number of leaves where highly significant difference (P≤0.01) was found.

Among the main problems encountered with crop plants, the most important one is represented by genetic erosion. At world level this issue has been debated within the Convention on Biodiversity adopted in UN Conference of Rio de Janeiro and The International Convention regarding Genetic Resources of Plants for Alimentation and Agriculture. In Europe, the regulations in this field are made by the European Directive 98/95 EEC. Across time tomatoes have suffered a heightened process of genetic diversity, phenomenon caused by industrialized farming which is based on the use of very uniform varieties with high productivity. The extension of green agriculture, has led to the use of landraces, because they respond best to the traditional farming systems. In Western Europe organizations of peasants had been founded and, they are involved in different activities aiming at conservation of traditional forms of agriculture and the use of old varieties. In Romania the institutions with continuous preoccupations in the field of vegetal genetic conservation, especially landraces, are the Gene Bank from Suceava and UASMV Cluj-Napoca. By using conservation methods (in situ and ex situ), the genetic erosion surely phenomenon of vegetal genetic resources, implicitly tomatoes, is reduced, the future generations will benefit from valuable genetic resources.

Improvement of tomato (Lycopersicon esculentum L.) for growth in saline soils is a major goal of tomato breeders. The aim of this study was to identify the genetic combining ability and genetics of salinity tolerance in tomato. Plant materials were grown under normal (NG) and salinity stress (SSG) conditions. Results showed that the genetic controlling mechanism of salinity-related traits and fruit weight is complex and that all genetic components of additive, non-additive and maternal are involved. The nature of gene action for fruit weight and salinity-related traits was significantly affected by salinity stress. Dominance and additive gene action were predominant under NG and SSG, respectively. Under NG, the best general combiner parent for fruit weight was P3 (salt-tolerant with moderate fruit yield). Under SSG, P1 (highly salt-tolerant with low fruit yield) was the best general combiner parent for fruit weight and exhibited high genetic combining ability for K+/Na+, lipoxygenase activity, proline, relative water content, total carbohydrate and cell membrane stability. With the high frequency of genes effective in salt tolerance, the P1 parent appeared as the best specific mating partner with other parents under SSG. Simultaneous selection for fruit weight and surrogate traits (cell membrane stability, proline and relative water content) in a population derived from the P1 × P5 (susceptible with high fruit yield) cross could result in a salt-tolerant tomato genotype.

Several leaves and shoot spot symptoms were observed on tomato (Lycopersicon esculentum Mill) plants at Zubair and Safwan farms in Basrah, Iraq. The symptomatic plant parts were collected from different varieties of tomato. The fungal pathogens were first isolated on PDA medium followed by a diagnosis procedure of the relevant fungi conducting phenotypic characterization and microscopic featuring. A molecular identification was applied by polymerase chain reaction technology using internal transcribed spacer sequences. All along an investigation on Cladosporium species, three species including C. cladosporioides, C. oxysporum, and C. uredinicola were identified and recorded as new potential pathogen for the first time in Iraq causing leaf spot and lesion of tomato plant. Pathogenicity examination of each isolated pathogens was performed against of tomato plant. The importance of the current research is to investigate and identify the fungal pathogens of tomato plants to find the best protocols to restrict their growth and damage.

BACKGROUND: Tomatoes (Lycopersicon esculentum Mill.) have very huge health-promoting benefits due to high nutritional composition; however, these fruits are potential reservoir of enteric food-borne pathogens causing major public health concerns. AIM: Characterization and phylo-analysis of implicated enteric bacteria strains in retailed Tomato fruits in southwest Nigeria were studied. METHODS: Ready to be retailed fresh tomato fruits were purchased from common food markets in southwest, Nigeria, which lies between latitudes 6° 21′ to 8° 30′ N and longitudes 2° 30′ to 5° 30′ E. Observation of sample storage potentials at different conditions and bio-typing of associated bacterial strains were carried out for consecutive 14 days. Enteric bacteria strains were genotyped with 16S rRNA assay and further profiled for antibiotic susceptibility to common antibiotics. High population rate frequently consume tomatoes. RESULTS: Early spoilage characterized with yellow fluid, fungal growth and visible lesions were observed at 25°C storage compare to few patches of lesion at 4°C after 14 days. Higher bacterial count of 4.0–7.18 Log CFU/g was recorded at ambient storage compare to refrigerated samples with more than 10% occurrence rate of Citrobacter spp., Klebsiella spp. and Enterobacter spp. Identified Citrobacter spp. and Klebsiella spp. showed 100% resistant to beta-lactam antibiotics (ceftazidime, cefuroxime, cefixime, ciprofloxacin, and amoxicillin-clavulanic acid). Two-resistant enteric bacteria strains, Klebsiella aerogenes B18 and Citrobacter freundii B27 obtained from Nigerian tomato clustered with Citrobacter strains in food (China), water strains (India, Poland, Malaysia), milk (Germany), and human fecal (China). CONCLUSION: Implicated multidrug-resistant enteric bacilli in retailed tomatoes can cause severe food-borne diseases which public oriented awareness, strategic farm to market surveillance are needed to be intensified.

The article considers studies designed to justify the types of pathogens of tomatoes that occur during vegetation and storage, and measures to combat them. The work was carried out in 2019 on a land plot near the rural districts of Babaykorgan, Zhuynek, and Issa of the Turkestan region. As it became known, the growth period of vegetable seedlings depends on the temperature of the soil. In our experience, the seed material was planted in the open ground in early April. The soil was very hot and moist. Depending on the culture and varietal characteristics, the sprouts appeared on 6-15 days after sowing. The results of phytopathological control showed that various varieties of all types of tomatoes were affected by phytopathogenic microorganisms. During the growing season, the most dangerous disease in tomato fruit was apical rot of the fruit, which led to a significant decrease in the yield. The leaves are widely developed early blight and verticillus. Late blight at the end of the growing season was observed in full on both leaves and fruits (developed during fruit storage). When growing tomato crops at two sites was dominated by late blight, early blight, verticillus, viral diseases and apical rot. Based on the results of phytopathological control, it can be concluded that the number of fungal diseases prevailed in plot 1, which is associated with frequent irrigation on this site, its shading and a large number of fruits on plants. Viral diseases prevailed in plot 2, which is explained by weeds clogging the plot.

Zinc oxide nanoparticles (ZnO-NPs) has been demonstrated to positively regulate plant tolerance to multiple environmental stresses. However, till date little information has been gained regarding the role of ZnO-NPs in the salt stress regulation in plants. Hence, the objective of our study was to investigate the role of ZnO-NPs in the regulation of salt tolerance in tomato (Lycopersicon esculentum Mill.). In this regard, the tomato plants were subjected to salt stress by using NaCl (150 mM) at the time of transplantation [15 days after sowing (DAS)]. Foliar application of ZnO-NPs at different levels viz., 10, 50 and 100 mg/L in the presence/absence of NaCl (150 mM) was carried out at 25 DAS and sampling was done at 35 DAS. Results of our study revealed that foliar spray of ZnO-NPs significantly increased shoot length (SL) and root length (RL), biomass, leaf area, chlorophyll content and photosynthetic attributes of tomato plants in the presence/absence of salt stress. Besides, the application of ZnO-NPs mitigates the negative impacts of salt stress on tomato growth, and enhanced protein content and antioxidative enzyme activity such as peroxidase (POX), superoxide dismutase (SOD) and catalase (CAT) under salt stress. In conclusion, the ZnO-NPs plays an important role in the alleviation of NaCl toxicity in tomato plants. Hence, the ZnO-NPs can be used to boost the growth performance and mitigate the adverse effects caused by NaCl in tomato.

Tomato (Lycopersicon esculentum Mill.) is attacked by various disease causing micro-organisms. Amongthem Fusarium wilt is the major destructive diseases of tomato. This study was conducted to test theefficacy of different fungicides i.e. metalaxyl+mancozeb, copper oxychloride, benalaxyl+mancozeb,carbendazim and mancozeb at different concentrations (2, 2.5, 3, 3.5, 4 g/litre water) against the fusariumwilt of tomato caused by Fusarium oxysporum f. sp. lycopersici and also to observe the impacts offungicides on plant height and yield under tunnel condition. The result revealed that copper oxychloridewas significantly effective in all its doses to control the Fusarium wilt of tomato, the most effective dose was 3 g/l where the disease severity was recorded 6.2 percent only, followed by metalaxyl+mancozeb (4g/l) in which the disease severity was recorded 9.6 percent, other fungicides also showed good result but mancozeb alone was not effective, however it had synergistic effect and could be used as basis with the other product to control the fusarium wilt. Two of the fungicides proved to be the most appropriate chemicals having no severe impacts on plant height and yield. The highest plant height was recorded 10.96 and 9.38 feet whereas the highest yield per plant was recorded 3.97 and 3.67 kg in case of copper oxychloride and metalaxyl+mancozeb respectively.

Tomato (Lycopersicon esculentum, Mill) is a rich source of vitamins, minerals and lycopene, which has many health benefits. However, its production is hampered by bacterial wilt caused by Ralstonia solanacearum resulting in significant yield losses. Use of chemicals in the control of plant pathogens has detrimental effects on humans and the environment in terms of leaving residues in soil which later find their way into underground waters. Therefore, it is desirable to find an alternative to chemical control of this bacterial pathogen. This study investigates the potential of native Bacillus thuringiensis (Bt) for biological control of Ralstonia solanacearum (Rs) under laboratory conditions. B. thuringiensis was isolated from cultivated soil, non- cultivated soil, stagnant water, sawdust, horse dung, grain dust, dead leaves and poultry manure. R. solanacearum was isolated from stem exudates of bacterial wilt infected plants and its pathogenicity assay was carried out using 2-week-old seedlings of Beske tomato variety. The Bt and R. solanacearum isolates were then characterized phenotypically. Bt isolates were further identified using endospore and parasporal staining techniques. All the Bt isolates were tested for in-vitro antagonistic activity on R. solanacearum using agar well diffusion method. Isolates Bt2, Bt16, Bt17, Bt32 and Bt34 were confirmed as Bacillus thuringiensis while isolate Rs was confirmed as R. solanacearum. Beske showed wilting symptoms from the fourth day of inoculation and eventual death of seedlings. The zone of inhibition exhibited ranged from 0.0 mm to 20.0 mm. Keywords: Bacillus thuringiensis, In-vitro, Bacterial wilt, Ralstonia solanacearum, Tomato

Today, human health has been adversely exposed to many inauspicious threats due to human activities. Evidently, ecotoxicological studies documented that industrial and agricultural wastes tremendously contribute to the contemporary environmental pollution. Meanwhile, the application of tannery sludge as fertilizer has been recently reported in Dawakin Kudu LGA of Kano State, Nigeria and this sludge was suspected to have contained a large quantity of toxic metals. This study was aimed to investigate the presence of toxic metals including Cd, Cr and Pb in the fruits of Tomato (Lycopersicon esculentum) grown with tannery sludge in the selected farms in the study area in order to ascertain their suitability for human consumption. The toxic metals (Cr, Cd and Pb) were found to be incorporated into the tissue of tomato

Tomato bacterial canker, caused by Clavibacter michiganensis subsp. michiganensis, is a serious tomato disease causing plant death and significant economic losses to the farmers. Several measures h...

Cadmium(II) adsorption was investigated on different adsorbents, namely Lycopersicon esculentum peel (LEP), Lycopersicon esculentum peel activated carbon (LEP-AC), Brassica botrytis leaves (BBL) and Brassica botrytis leaves activated carbon (BBL-AC). Batch experiments were performed to study the effects of pH, temperature, initial metal ion concentration, contact time and adsorbent dosage. The study concluded that the incorporation of activated carbon to the parental adsorbents leads to accelerate the rate of adsorption. The process was exothermic and due to the exothermic nature, the percent adsorption was decreased by increasing temperature. The experimental data were evaluated by Lagergren and Ho’s kinetic models by taking into account the adsorption of Cd by different adsorbents.

The biofertilizers are commonly called as microbial inoculants which are capable of mobilizing important nutritional elements in the soil from non-usable to usable form by the crop plants through their biological processes. The present investigation was carried out to study the application of soil organic amendments Azospirillum, Rhizobium, Phosphobacteria and Pseudomonas fluorescens significantly increased morphological, photosynthetic and biochemical parameters on tomato (Lycopersicon esculentum Mill.) at all growth stages. It can be concluded that biofertilizer manure can be used for quick stabilization of soil fertility as well as biological activities, which is in turn help in nutrient availability during transition periods. Finally, it was observed that the plants treated with experimental biofertilizer Rhizobium showed excellent results in the morphological and biochemical parameters as compared to the other biofertilizers and also control.

Sufficient quantity and quality of water are essential for vegetable production. Evaluating the individual and combined effects of drought and salt stress on plant establishment may help inform management of plant production in terms of stabilizing yield. This study investigates the effects of drought and salt stress on plant growth, nutrition elements, and select physiological parameters of tomato (Lycopersicon esculentum L. cv. H2274) through a pot experiment under greenhouse conditions. Drought stress was applied to tomato seedlings at 3 different levels of irrigation: D0 (control), D1, and D2 at 100%, 75%, and 50% field capacity, respectively. Salinity stress was applied at 3 different doses S0, S1, and S2 at 0 mM, 100 mM, and 150 mM NaCl, respectively. The effects of salinity, drought and their co-occurring stresses on growth, physiological and photosynthetic properties of tomato seedlings were statistically significant. Photosynthetic rate (Pn), stomatal conductance (gs), chlorophyll reading value (CRV), and plant dry weight (DW) decreased with salt and drought treatments. However, electrolyte leakage (EL) of the leaves increased with increasing salt and drought treatments. Fresh weight (FW) of the tomato seedlings decreased 67% under the 150 mM NaCl treatment (S-2), and decreased 69% under severe drought (D2); however, the interactive effects of these two treatments together (S2D2) resulted in an 80% decrease in FW. Furthermore, dry weight (DW) decreased 80% in the S2D2 treatment compared to the control. In general, both drought and salinity altered the mineral nutrient composition by decreasing N, P, K, Fe, Ca and Z content of the tomato seedlings, but Na, B and Cl content increased. These results show that effects of salinity and drought on tomato seedlings were less detrimental for plant establishment than the combined effects of the two stresses together.

Tomato is a popular and extensively cultivated vegetable among the promising commodities in horticultural production in Kenya. It provides a wide variety of nutrients with many health-related benefits. Despite the importance, its yield and net economic benefits is limited by the cultural practices applied by farmers. There is limited knowledge on the effect of integrating pruning and earthing up on tomato yield and net economic benefit. This study investigated the effect of integration of pruning and earthing up on the growth and yield of tomatoes. A split-plot experimental design, arranged in a Randomized Complete Block Design, with three replications was used. The study investigated two factors i.e. pruning system in the main plot (single stem, double stem, and triple stem) and earthing up in sub-plots. (0 cm, 10 cm, 20 cm, and 30 cm). Fruit yield data was taken after each harvest. Data were analysed using SAS version 9.4 and significant means were separated using the least significant difference at α = .05. The findings of the study revealed that earthing up and pruning system had a significant (p ˂ .05) effect on tomato yield and net economic benefit. Triple stem pruning system, earthing up to 30 cm had the highest fruit yield with 21.82 tonnes/hectare in cultivation 1 and 21.84 tonnes/hectare in cultivation 2. The findings also revealed that triple stem pruning system, earthing up to 30 cm had the highest net economic benefit per hectare in both cultivation 1 and cultivation 2. To improve tomato yield and consequently improve net economic benefit, farmers are encouraged to consider triple stem pruning system and earthing up to level 30 cm.

The purpose of this study is to determine the effects of selenium (Se) on cherry tomato fruits under cadmium (Cd) stress. Methods: Se (2.5 μM) was applied to hydroponic solution with different concentrations of Cd (10 μM and 50 μM). The yield, quality, mineral elements, and Cd contents of cherry tomato was determined. Ten micromolars of Cd had no effect on the appearance and quality of cherry tomato, while 50 μM Cd significantly reduced the content of vitamin C, lycopene, soluble solids, and soluble sugars in the fruits. Cd stress significantly reduced cherry tomato fruit yield and also inhibited the accumulation of mineral elements such as calcium, magnesium, and iron in the fruits, and these inhibitory effects increased with the increase of Cd concentration. Besides, Se application significantly increased fruit yield by 8.38% and 8.56% under 10 μM and 50 μM Cd stress, respectively, when compared with plants grown without Se. Exogenous application of Se also significantly increased vitamin C, lycopene, and soluble sugar contents of fruit under 50 μM Cd stress and effectively promoted the accumulation of calcium, iron, copper, and zinc in the fruits. In addition, Se significantly reduced the Cd contents in roots, stems, and leaves of cherry tomato plants under 10 μM and 50 μM Cd stress, thereby leading to 12.85% and 12.48% respective decrease in the Cd content in fruits when compared with plants grown without Se. Therefore, applying Se can effectively improve the yield and quality of cherry tomato fruits under Cd stress and also reduce the Cd content in fruits.

Although salinity stress adversely affects plant function, manipulation of the rhizosphere may alleviate those negative impacts. We examined whether adjustment of rhizosphere pH, unadjusted control...

Tomato (Lycopersicon esculentum Mill; Solanaceae) is one of the major horticultural crops cultivated in India. In order to ensure year-round availability of tomatoes, farmers are shifting to protected cultivation practices. Although the name ‘protected cultivation’ suggests freedom from biotic as well as abiotic hindrances in crop cultivation, incidence of pests have been reported in crops grown under polyhouses. This causes net house cultivators to use more chemical insecticides. Thus, an environmentally safe and economical method, comprising sticky traps, was evaluated for the management of sucking pests of tomato grown under protected conditions. Sticky traps of different shapes (rectangle, circle and triangle) and colours (yellow, blue and silver) were evaluated against sucking pests of tomato in 2018. The sticky trap, which found most efficient during 2018, was installed at different rates (2, 4 and 6 traps/250 m2) in the year 2019. Among the various sticky traps evaluated, yellow colour and rectangular trap proved best among the other shapes and colours against the aphids and whiteflies infesting tomato under protected conditions. On standardization of yellow-rectangular trap in 2019, a rate of installation of 4, 6 and 2, 4, 6 traps per 250 m2 were effective in reducing the population of aphids and whiteflies, respectively. Hence it could be concluded that yellow-rectangular sticky trap installed at a rate of 4 per 250 m2 proved effective against sucking pests of tomato grown under protected conditions.

Background and Objectives: Plant Growth-promoting Bacteria (PGPB) can replace the dangerous chemical fertilizers and pesticides. The aim of this study was to isolate the PGPBs for Lycopersicon esculentum plant and to determine the appropriate volume for inoculation. Materials and Methods: Plants samples were collected from tomato fields. Nitrogen fixing-PGPBs were isolated from rhizoplane and rhizosphere. Five isolates were screened based on their growth abilities and examined for PGPB traits including phosphate solubilization, and IAA, ammonia and HCN production. After high cell density cultivation, the cells were separated by centrifugation and freeze dried after resuspension in cryoprotectant. The powders were inoculated into sterile soil with a dose of 106 , 107 and 108 CFUs/g. Tomato (Lycopersicon esculentum) seeds were sown in soil and after 42 days the shoot length was measured. Results: Most of the potent PGPBs with high growth capacity were isolated from rhizoplane. Maximum phosphate solubilization was 289.7 µg/ml by NFB12 which isolated from rhizoplane. This strain produced the maximum level of IAA. NFB12 produced ammonia without the ability of production of HCN. This strain enhanced shoot length in dosed dependent manner. Surprisingly, inoculation of soil with 108 CFUs/g dramatically decreased the shoot length by 21%. Based on molecular approach NFB12 was identified as Bacillus megaterium. Conclusion: Isolation of specific PGPBS is recommended for sustainable plant production. Our results showed that NBF12 improves tomato plant growth and its effect on tomato plant growth is does dependent. Maximum growth rate of tomato was observed with 107 CFUs/g soil inoculation of NFB12 while higher inoculation showed negative effect.

Tomato (Lycopersicon esculentum Mill.) are considered major and important globally vegetable crops and in Egypt in particular. Tissue culture techniques have encouraged the utilization of mutation methods in crop improvement. The mutation induction in vegetative crops through tissue culture may be the optimal method to improve these crops. Induced genetic variation in tomato plantlets by using gamma radiation and identified these changes through SCoT and ISSR markers. Egyptian tomato cultivar Idkawy explant was cultured onto MS medium supplemented with 0.2 mg-l BAP. The resulted plantlets were irradiated with γ radiation doses (50, 100, 150, 200 or 250 Gy). The survival, growth rate, and mean of shoot length were decreased with increasing gamma radiation dose. The irradiated plantlets survival percentages were ranged from 78.75% to (50 Gy) and 18.75% to (250 Gy), whereas, the shoot length decreased by a rate of 2.71 cm for the dose (50 Gy) and 1.2 cm for dose (250 Gy). Genetic diversity was evaluated by SCoT and ISSR markers using ten primers for each. It was noticed that the polymorphism percentage mean of SCoT marker (60.53%) is higher than the ISSR marker (39.6). The PIC values average for both markers SCoT and ISSR were 0.429 and 0.347, as well, MI values were 0.345 and 0.156, respectively. On the other hand, the effective no. of alleles (Ne), Nei’s genetic diversity (H) and Shannon’s information index (I) parameters, it was found that the dose 100 Gy caused the highest genetic variation compared with other doses using SCoT marker, however, in ISSR marker was dose of 150 Gy the highest dose for induced genetic variation. The obtained results demonstrate that SCoT marker was more accurate and efficient than ISSR marker for distinguishing and genetic variation analysis of irradiated tomato plantlets. The relationships within treatments were assessed through cluster analysis (UPGMA) based on SCoT and ISSR analysis.

Tomato is one of the most remunerable and widely grown vegetables in the world. The objective of this study was to evaluate tomato varieties for nematode and Ralstonia diseases resistance and productivity.