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254 results on '"Lv, Zhiyue"'

14. Apoptosis and necroptosis of mouse hippocampal and parenchymal astrocytes, microglia and neurons caused by Angiostrongylus cantonensis infection

Author

Zhang Mengying, Xu Yiyue, Pan Tong, Hu Yue, Yanin Limpanont, Huang Ping, Kamolnetr Okanurak, Wu Yanqi, Paron Dekumyoy, Zhou Hongli, Dorn Watthanakulpanich, Wu Zhongdao, Wang Zhi, and Lv Zhiyue

Subjects
Angiostrongylus cantonensis, Pathogenesis, Apoptosis, Necroptosis, Infectious and parasitic diseases, RC109-216
Abstract

Abstract Background Angiostrongylus cantonensis has been the only parasite among Angiostrongylidae to cause human central nervous system infection characterized by eosinophilic meningitis or meningoencephalitis. The mechanism of the extensive neurological impairments of hosts caused by A. cantonensis larvae remains unclear. The aim of the present study was to investigate apoptosis, necroptosis and autophagy in the brains of mice infected with A. cantonensis, which will be valuable for better understanding the pathogenesis of angiostrongyliasis cantonensis. Methods Functional and histological neurological impairments of brain tissues from mice infected with A. cantonensis were measured by the Morris water maze test and haematoxylin and eosin (H&E) staining, respectively. The transcriptional and translational levels of apoptosis-, necroptosis- and autophagy-related genes were quantified by quantitative real-time polymerase chain reaction (RT-PCR), and assessed by western blot and immunohistochemistry (IHC) analysis. Apoptotic and necroptotic cells and their distributions in infected brain tissues were analysed by flow cytometry and transmission electron microscopy (TEM). Results Inflammatory response in the central nervous system deteriorated as A. cantonensis infection evolved, as characterized by abundant inflammatory cell infiltration underneath the meninges, which peaked at 21 days post-infection (dpi). The learning and memory capacities of the mice were significantly decreased at 14 dpi, indicating prominent impairment of their cognitive functions. Compared with those of the control group, the mRNA levels of caspase-3, -4, -6, and RIP3 and the protein levels of caspase-4, cleaved caspase-3, cleaved caspase-6, RIP3, and pRIP3 were obviously elevated. However, no changes in the mRNA or protein levels of FADD, Beclin-1 or LC3B were evident, indicating that apoptosis and necroptosis, but not autophagy, occurred in the brain tissues of mice infected with A. cantonensis. The quantitative RT-PCR, western blot, IHC, flow cytometry and TEM results further revealed the apoptotic and necroptotic microglia, astrocytes and neurons in the parenchymal and hippocampal regions of infected mice. Conclusions To our knowledge, we showed for the first time that A. cantonensis infection causes the apoptosis and necroptosis of microglia and astrocytes in the parenchymal and hippocampal regions of host brain tissues, further demonstrating the pathogenesis of A. cantonensis infection and providing potential therapeutic targets for the management of angiostrongyliasis.

Published
2017
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29. Evaluation of indirect-ELISA using eluted antigens from Trichinella spiralis muscle larvae for diagnosis of swine trichinellosis

Author

Utsanee Supcharoengoon, Onrapak Reamtong, Paron Dekumyoy, Dorn Watthanakulpanich, Yanin Limpanont, Lv Zhiyue, Salisa Chaimon, Pongsakorn Martviset, and Poom Adisakwattana

Subjects
Swine Diseases, Swine, Veterinary (miscellaneous), Muscles, Trichinella, Antibodies, Helminth, Enzyme-Linked Immunosorbent Assay, Trichinellosis, Infectious Diseases, Insect Science, Antigens, Helminth, Immunoglobulin G, Larva, Animals, Humans, Parasitology, Trichinella spiralis
Abstract

Trichinellosis is caused by Trichinella spiralis muscle larvae (TsML), which is transmitted to human when they eat infected raw or undercooked meat. T. spiralis infection is detected by an enzyme-linked immunosorbent assay (ELISA) using excretory-secretory antigens (ESAg); however, the preparation of ESAg is challenging, and yields are low, which hampers screening efforts. In this study, crude somatic antigens (CSAg) of TsML with molecular weights (MWs) of 43, 79 and 101 kDa have been identified in swine trichinellosis sera with less cross-reaction with uninfected sera and other parasitic infected sera. After that, the CSAg at MWs of 43, 79 and 101 kDa (TsCSAg-43, TsCSAg-79, and TsCSAg-101, respectively) were isolated from sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The eluted antigens were analyzed by IgG-ELISA for sensitivity and specificity, and specific antigens from the three regions were identified by two-dimensional polyacrylamide gel electrophoresis (2-DE) and liquid chromatography-tandem mass spectrometry (LC-MS-MS). The sensitivity of IgG-ELISA using the three eluted antigens was 100% with specificities of 97.77%, 95.54%, 90.63% and for TsCSAg-43, TsCSAg-79, and TsCSAg-101, respectively. The LC-MS-MS results of immunomics showed that 18/20 spots of the antigens with MWs of 43, 79, and 101 kDa represent 11 different proteins identified. TsCSAg-43 showed the highest specificity, indicating that the specific proteins identified, including 45 kDa antigen-trichina [fragment], DNA topoisomerase 2-alpha antigen targeted by protective antibodies, and a conserved hypothetical protein (gi339234223), should be developed and produced in large volumes for further immunodiagnostic studies.

Published
2022

30. Assessing the efficacy of soapberry (Sapindus rarak) crude extract for controlling giant African land snail (Lissachatina fulica)

Author

Koysap, Lueangkaew, primary, Ruangsittichai, Jiraporn, additional, Ampawong, Sumate, additional, Kongkiatpaiboon, Sumet, additional, Worakhunpiset, Suwalee, additional, Thaenkham, Urusa, additional, Chusongsang, Yupa, additional, Lv, Zhiyue, additional, Mongkolthanawat, Somsak, additional, and Limpanont, Yanin, additional

Published
2022
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47. Apoptosis and necroptosis of mouse hippocampal and parenchymal astrocytes, microglia and neurons caused by Angiostrongylus cantonensis infection

Author

Huang Ping, Xu Yiyue, Kamolnetr Okanurak, Dorn Watthanakulpanich, Pan Tong, Lv Zhiyue, Paron Dekumyoy, Wu Yanqi, Hu Yue, Wu Zhongdao, Yanin Limpanont, Zhang Mengying, Zhou Hongli, and Wang Zhi

Subjects
0301 basic medicine, Pathology, Apoptosis, Pathogenesis, Hippocampus, Mice, 0302 clinical medicine, FADD, Neurons, Behavior, Animal, medicine.diagnostic_test, biology, Microglia, Histocytochemistry, Meningoencephalitis, Flow Cytometry, Immunohistochemistry, Angiostrongylus cantonensis, Infectious Diseases, medicine.anatomical_structure, Necroptosis, Locomotion, medicine.medical_specialty, Blotting, Western, Central nervous system, Real-Time Polymerase Chain Reaction, lcsh:Infectious and parasitic diseases, Necrosis, 03 medical and health sciences, Microscopy, Electron, Transmission, Western blot, Autophagy, medicine, Animals, lcsh:RC109-216, Strongylida Infections, Research, Gene Expression Profiling, biology.organism_classification, medicine.disease, Disease Models, Animal, 030104 developmental biology, Astrocytes, biology.protein, Angiostrongyliasis, Parasitology, 030217 neurology & neurosurgery
Abstract

Background Angiostrongylus cantonensis has been the only parasite among Angiostrongylidae to cause human central nervous system infection characterized by eosinophilic meningitis or meningoencephalitis. The mechanism of the extensive neurological impairments of hosts caused by A. cantonensis larvae remains unclear. The aim of the present study was to investigate apoptosis, necroptosis and autophagy in the brains of mice infected with A. cantonensis, which will be valuable for better understanding the pathogenesis of angiostrongyliasis cantonensis. Methods Functional and histological neurological impairments of brain tissues from mice infected with A. cantonensis were measured by the Morris water maze test and haematoxylin and eosin (H&E) staining, respectively. The transcriptional and translational levels of apoptosis-, necroptosis- and autophagy-related genes were quantified by quantitative real-time polymerase chain reaction (RT-PCR), and assessed by western blot and immunohistochemistry (IHC) analysis. Apoptotic and necroptotic cells and their distributions in infected brain tissues were analysed by flow cytometry and transmission electron microscopy (TEM). Results Inflammatory response in the central nervous system deteriorated as A. cantonensis infection evolved, as characterized by abundant inflammatory cell infiltration underneath the meninges, which peaked at 21 days post-infection (dpi). The learning and memory capacities of the mice were significantly decreased at 14 dpi, indicating prominent impairment of their cognitive functions. Compared with those of the control group, the mRNA levels of caspase-3, -4, -6, and RIP3 and the protein levels of caspase-4, cleaved caspase-3, cleaved caspase-6, RIP3, and pRIP3 were obviously elevated. However, no changes in the mRNA or protein levels of FADD, Beclin-1 or LC3B were evident, indicating that apoptosis and necroptosis, but not autophagy, occurred in the brain tissues of mice infected with A. cantonensis. The quantitative RT-PCR, western blot, IHC, flow cytometry and TEM results further revealed the apoptotic and necroptotic microglia, astrocytes and neurons in the parenchymal and hippocampal regions of infected mice. Conclusions To our knowledge, we showed for the first time that A. cantonensis infection causes the apoptosis and necroptosis of microglia and astrocytes in the parenchymal and hippocampal regions of host brain tissues, further demonstrating the pathogenesis of A. cantonensis infection and providing potential therapeutic targets for the management of angiostrongyliasis.

Published
2017

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