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Your search keyword '"Lv, Enguang"' showing total 17 results
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17 results on '"Lv, Enguang"'

7. Near‐Infrared‐Responded High Sensitivity Nanoprobe for Steady and Visualized Detection of Albumin in Hepatic Organoids and Mouse Liver

Author

Liu, Guofeng, primary, Wei, Jinsong, additional, Li, Xiaoyu, additional, Tian, Meng, additional, Wang, Zhenxing, additional, Shen, Congcong, additional, Sun, Wan, additional, Li, Chonghui, additional, Li, Xuewen, additional, Lv, Enguang, additional, Tian, Shizheng, additional, Wang, Jihua, additional, Xu, Shicai, additional, and Zhao, Bing, additional

Published
2022
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10. Incorporation of Silver-Embedded Carbon Nanotubes Coated with Tannic Acid into Polyamide Reverse Osmosis Membranes toward High Permeability, Antifouling, and Antibacterial Properties

Author

Zhao, Afang, primary, Zhang, Na, additional, Li, Qiang, additional, Zhou, Lianwen, additional, Deng, Huining, additional, Li, Zhenghua, additional, Wang, Yi, additional, Lv, Enguang, additional, Li, Zhen, additional, Qiao, Mei, additional, and Wang, Jihua, additional

Published
2021
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13. Magnetic‐Field‐Driven Extraction of Bioreceptors into Polymeric Membranes for Label‐Free Potentiometric Biosensing.

Author

Lv, Enguang, Li, Yanhong, Ding, Jiawang, and Qin, Wei

Subjects
*LABELS, *PATTERN recognition systems, *MAGNETIC fields, *STAPHYLOCOCCUS aureus, *BIOLOGICAL assay, *POLYMERIC membranes
Abstract

We report here the concept of a magnetically controlled extraction of hydrophilic bioreceptors into polymeric membranes for bioassays. The potentiometric assay relies on the intrinsic charges of an antimicrobial peptide and its unique recognition abilities, which can eliminate the probe labeling and indicator addition. The target binding event could effectively prevent the extraction of the peptide into the polymeric membrane doped with an ion exchanger, thus resulting in a potential change. The potentiometric response properties of the peptide assembled on magnetic beads can be dynamically controlled and modulated by applying a magnetic field. Staphylococcus aureus, as a model of food‐borne pathogens, was measured at levels down to 10 CFU mL−1. Based on this sensing strategy, a potentiometric array was developed for the pattern recognition of bacteria. The proposed general platform can be used for potentiometric biosensing using other hydrophilic bioreceptors. [ABSTRACT FROM AUTHOR]

Published
2021
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17. Potentiometric Detection of Listeria monocytogenesvia a Short Antimicrobial Peptide Pair-Based Sandwich Assay

Author

Lv, Enguang, Ding, Jiawang, and Qin, Wei

Abstract

Peptide-based sandwich assays are promising tools in molecular detection, but may be restricted by the availability of “pairs” of affinity peptides. Herein, a new potentiometric sandwich assay for bacteria based on peptide pairs derived from an antimicrobial peptide (AMP) ligand is demonstrated. As a model, the original AMP with a well-defined structure for Listeria monocytogenes(LM) can be split into two fragments to serve as the peptide pairs for the sandwich assay. The recognition and binding of the short peptide pairs to the target can be verified by circular dichroism, flow cytometry, fluorometry, and optical microscopy. The potentiometric magnetic bead-based sandwich assay is designed by using horseradish peroxidase as a label. The enzyme can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine with H2O2to induce a potential change on a polymeric membrane ion-selective electrode. Under optimal conditions, the concentration of LM can be determined potentiometrically in a linear range of 1.0 × 102to 1.0 × 106CFU mL–1with a detection limit of 10 CFU mL–1(3σ). The proposed sensing strategy expands the applications of peptides in the field of bioassays.

Published
2018
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