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2. Pathophysiology of Burn Wounds

Author

Nischwitz, Sebastian P., Luze, Hanna, Kamolz, Lars-Peter, Nischwitz, Sebastian P., editor, Kamolz, Lars-Peter, editor, and Branski, Ludwik K., editor

Published
2024
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8. Tattoo Pigment Biokinetics in vivo in a 28-Day Porcine Model: Elements Undergo Fast Distribution to Lymph Nodes and Reach Steady State after 7 Days.

Author

Cambiaso-Daniel, Janos, Luze, Hanna, Meschnark, Silvia, Fink, Julia, Schreiver, Ines, Rappl, Thomas, Goessler, Walter, Kotzbeck, Petra, and Kamolz, Lars-Peter

Subjects
LYMPH nodes, MATRIX-assisted laser desorption-ionization, COPPER, TATTOOING, PIGMENTS, BODY piercing
Abstract

Introduction: Pigments of tattoo inks may over time migrate to other parts of the body. Inks kinetics are still poorly understood and little studied. The aim of this first study was to investigate the kinetics of tattoo inks pigment in tattooed porcine skin, which is closer to human skin than mouse skin studied in the past. Methods: Three animals were tattooed on the inner thigh and one animal served as untreated control. Skin biopsies were taken on days 7, 14, and 28 after tattooing. Animals were sacrificed on day 28 and homogenate samples of the liver, spleen, kidney, and brain, as well the local lymph nodes were prepared. All samples were analyzed for ink components using inductively coupled plasma-mass spectrometry. The ink itself was characterized by dynamic light scattering and matrix-assisted laser desorption-ionization mass analysis. Results: Titanium (212 g/kg), copper (6 mg/kg), aluminum (1 mg/kg), zirconium (1 mg/kg), and chromium (3 mg/kg) were found in the ink. Significant deposits of ink elements were detected in the tattooed skin when compared to non-tattooed skin from the same animal (mean ± standard deviation: titanium 240 ± 81 mg/kg, copper 95 ± 39 mg/kg, aluminum 115 ± 63 mg/kg, zirconium 23 ± 12 mg/kg, and chromium 1.0 ± 0.2 mg/kg; p < 0.05). Lymph node concentrations of titanium, copper, aluminum, zirconium, and chromium were 42 ± 2 mg/kg, 69 ± 25 mg/kg, 49 ± 18 mg/kg, 0.3 ± 0.2 mg/kg, 0.5 ± 0.2 mg/kg, respectively. Conclusion: Deposits in skin were unchanged from days 7–28 indicating no redistribution or elimination. No significant deposits of ink elements were found in the liver, spleen, kidney, and brain. In conclusion, our findings confirmed distribution of elements from tattoos to regional lymph nodes, but neither to excretory organs, e.g., liver and kidney, nor to spleen and brain. Thus systemic internal organ exposure was not found. [ABSTRACT FROM AUTHOR]

Published
2024
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11. Reconstructive and Aesthetic Surgeries on the Female Genitalia. Guideline of the DGGG, DGPRÄC, OEGGG and SGGG (S2k-Level, AWMF Registry No. 009/019, May 2022)

Author

Beckmann, Matthias W., additional, Borkenhagen, Ada, additional, Fahlbusch, Christine, additional, Gold, Daniela, additional, Hoffmann, Juergen, additional, Löhrs, Bettina, additional, Luze, Hanna, additional, Mirastschijski, Ursula, additional, OʼDey, Dan M., additional, Pöschke, Patrik, additional, Remmel, Eugenia, additional, Schaefer, Dirk, additional, Schulmeyer, Carla E., additional, Schuster, Tobias, additional, Sohn, Michael, additional, von Fritschen, Uwe, additional, and Beier, Justus P., additional

Published
2023
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12. Reconstructive and Aesthetic Surgeries on the Female Genitalia. Guideline of the DGGG, DGPRÄC, OEGGG and SGGG (S2k-Level, AWMF Registry No. 009/019, May 2022)

Author

Beckmann, Matthias W., Borkenhagen, Ada, Fahlbusch, Christine, Gold, Daniela, Hoffmann, Juergen, Löhrs, Bettina, Luze, Hanna, Mirastschijski, Ursula, OʼDey, Dan M., Pöschke, Patrik, Remmel, Eugenia, Schaefer, Dirk, Schulmeyer, Carla E., Schuster, Tobias, Sohn, Michael, von Fritschen, Uwe, and Beier, Justus P.

Abstract

Aim This official guideline was coordinated and published by the German Society for Gynaecology and Obstetrics (DGGG), the German Society for Plastic, Reconstructive and Aesthetic Surgery (DGPRÄC), the Austrian Society for Gynaecology and Obstetrics (OEGGG), and the Swiss Society for Gynaecology and Obstetrics (SGGG). The guideline aims to provide a consensus-based overview of reconstructive and aesthetic surgeries on female genitalia based on an evaluation of the relevant literature. Methods This S2k-guideline was developed by representative members from different medical professions on behalf of the guidelines commission of the DGGG, DGPRÄC, OEGGG and SGGG using a structured consensus process. Recommendations Statements and recommendations on the epidemiology, aetiology, classification, symptoms, diagnosis, and treatment of acquired changes of the external genitalia are presented and special situations are discussed.

Published
2023

13. Radiological Influencing Factors in the Diagnosis of Painful Habitual Instability of the Thumb Basal Joint as a Precursor of Carpometacarpal Arthritis of the Thumb—A Retrospective Study

Author

Winter, Raimund, primary, Hasiba-Pappas, Sophie, additional, Kamolz, Lars-P., additional, Tschauner, Sebastian, additional, Bamer, Oskar, additional, Tuca, Alexandru Cristian, additional, Luze, Hanna, additional, Nischwitz, Sebastian P., additional, Michelitsch, Birgit, additional, Friedl, Herwig, additional, Lumenta, David Benjamin, additional, and Girsch, Werner, additional

Published
2023
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14. Management of Severe Burn Wounds Colonized With Multi-resistant Pseudomonas aeruginosaand FusariumUsing Marine Omega3 Wound Matrix in a Female Victim of War

Author

Smolle, Christian, Holzer-Geissler, Judith C J, Auinger, Daniel, Mykoliuk, Iurii, Luze, Hanna, Nischwitz, Sebastian P, and Kamolz, Lars-Peter

Abstract

War-related burns are common injuries, also among the civilian population. Additional trauma such as fractures or shrapnel wounds may add significant morbidity. Burn injuries in war zones are furthermore frequently undertreated and hence prone to complications. We report a case of a young female victim of war, whose severely infected burn wounds could be successfully healed using a combination of targeted antimicrobial therapy, wound conditioning using decellularized fish skin, and subsequent skin grafting.

Published
2024
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16. Introducing the “SIMline”—A Simulation Course in the Management of Severe Burns as a Tool in Undergraduate Medical Education

Author

Sawetz, Isabel, primary, Hasiba-Pappas, Sophie, additional, Kamolz, Lars-Peter, additional, Holzer-Geissler, Judith C. J., additional, Tuca, Alexandru Cristian, additional, Lumenta, David Benjamin, additional, Wegscheider, Thomas, additional, Luze, Hanna, additional, Nischwitz, Sebastian P., additional, and Winter, Raimund, additional

Published
2023
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17. The Role of Local Inflammation and Hypoxia in the Formation of Hypertrophic Scars—A New Model in the Duroc Pig

Author

Nischwitz, Sebastian P., primary, Fink, Julia, additional, Schellnegger, Marlies, additional, Luze, Hanna, additional, Bubalo, Vladimir, additional, Tetyczka, Carolin, additional, Roblegg, Eva, additional, Holecek, Christian, additional, Zacharias, Martin, additional, Kamolz, Lars-Peter, additional, and Kotzbeck, Petra, additional

Published
2022
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19. Suction Drain Volume following Axillary Lymph Node Dissection for Melanoma—When to Remove Drains? A Retrospective Cohort Study

Author

Winter, Raimund, primary, Tuca, Alexandru, additional, Wurzer, Paul, additional, Schaunig, Caroline, additional, Sawetz, Isabelle, additional, Holzer-Geissler, Judith C. J., additional, Gmainer, Daniel Georg, additional, Luze, Hanna, additional, Friedl, Herwig, additional, Richtig, Erika, additional, Kamolz, Lars-Peter, additional, and Lumenta, David Benjamin, additional

Published
2022
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25. Personal protective equipment in the COVID-19 pandemic and the use of cooling-wear as alleviator of thermal stress

Author

Luze, Hanna, Nischwitz, Sebastian P., Kotzbeck, Petra, Fink, Julia, Holzer, Judith C. J., Popp, Daniel, and Kamolz, Lars-Peter

Subjects
Concentration, SARS-CoV-2, COVID-19, Thermal stress, Pilot Projects, General Medicine, Austria, Cooling wear, PPE, Humans, Original Article, Surgery, Plastic, Pandemics, Personal Protective Equipment
Abstract

Summary Background High temperatures at workplaces lead to health-related risks and premature exhaustion. The coronavirus disease 2019 (COVID-19) pandemic requires many health professionals to perform under unfavorable conditions. Personal protective equipment (PPE) causes thermal stress and negatively affects performance. Patients, materials and methods This pilot project investigated the effects of PPE and additional cooling wear on physiological parameters and concentration of six healthy staff members of the Plastic Surgery Department of the Medical University of Graz, Austria during simulated patient care. In this study two 1‑hour cycles with patient care-related tasks with PPE and PPE + cooling-wear, respectively, were conducted. A third cycle with scrubs exclusively served as baseline/negative control. The assessment occurred immediately pre-cycles and post-cycles. Results Pre-cycle assessments showed no significant differences between the cycles. After PPE cycle, increased physical stress levels and decrements in concentration capacity were observed. Physiological parameters were significantly less affected in the cooling cycle, while concentration capacity slightly increased. Conclusion COVID-19 PPE causes considerable thermal stress, ultimately affecting human performance. As opportunity to withstand thermal stress, and improve patients’ and professionals’ safety, cooling-wear can be considered relevant. Medical personnel performing in exceptional situations may particularly benefit from further development and investigation of cooling strategies.

Published
2020

28. Platelet-Rich Plasma in Plastic Surgery: A Systematic Review

Author

Hasiba-Pappas, Sophie K., primary, Tuca, Alexandru Cristian, additional, Luze, Hanna, additional, Nischwitz, Sebastian P., additional, Zrim, Robert, additional, Geißler, Judith C.J., additional, Lumenta, David Benjamin, additional, Kamolz, Lars-P., additional, and Winter, Raimund, additional

Published
2022
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29. The Role of Local Inflammation and Hypoxia in the Formation of Hypertrophic Scars—A New Model in the Duroc Pig.

Author

Nischwitz, Sebastian P., Fink, Julia, Schellnegger, Marlies, Luze, Hanna, Bubalo, Vladimir, Tetyczka, Carolin, Roblegg, Eva, Holecek, Christian, Zacharias, Martin, Kamolz, Lars-Peter, and Kotzbeck, Petra

Subjects
HYPERTROPHIC scars, WOUND healing, HYPOXEMIA, INFLAMMATION, THERAPEUTICS, GENE expression
Abstract

Hypertrophic scars continue to be a major burden, especially after burns. Persistent inflammation during wound healing appears to be the precipitating aspect in pathologic scarring. The lack of a standardized model hinders research from fully elucidating pathophysiology and therapy, as most therapeutic approaches have sparse evidence. The goal of this project was to investigate the mechanisms of scar formation after prolonged wound inflammation and to introduce a method for generating standardized hypertrophic scars by inducing prolonged inflammation. Four wound types were created in Duroc pigs: full-thickness wounds, burn wounds, and both of them with induced hyperinflammation by resiquimod. Clinical assessment (Vancouver Scar Scale), tissue oxygenation by hyperspectral imaging, histologic assessment, and gene expression analysis were performed at various time points during the following five months. Native burn wounds as well as resiquimod-induced full-thickness and burn wounds resulted in more hypertrophic scars than full-thickness wounds. The scar scale showed significantly higher scores in burn- and resiquimod-induced wounds compared with full-thickness wounds as of day 77. These three wound types also showed relative hypoxia compared with uninduced full-thickness wounds in hyperspectral imaging and increased expression of HIF1a levels. The highest number of inflammatory cells was detected in resiquimod-induced full-thickness wounds with histologic features of hypertrophic scars in burn and resiquimod-induced wounds. Gene expression analysis revealed increased inflammation with only moderately altered fibrosis markers. We successfully created hypertrophic scars in the Duroc pig by using different wound etiologies. Inflammation caused by burns or resiquimod induction led to scars similar to human hypertrophic scars. This model may allow for the further investigation of the exact mechanisms of pathological scars, the role of hypoxia and inflammation, and the testing of therapeutic approaches. [ABSTRACT FROM AUTHOR]

Published
2023
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39. The successful use of polylactide wound dressings for chronic lower leg wounds: A retrospective analysis.

Author

Nischwitz, Sebastian P., Popp, Daniel, Shubitidze, David, Luze, Hanna, Zrim, Robert, Klemm, Klaus, Rapp, Matthias, Haller, Herbert L., Feisst, Manuel, and Kamolz, Lars‐Peter

Subjects
TREATMENT of diabetic foot, WOUND healing, RESEARCH, CHRONIC wounds & injuries, RETROSPECTIVE studies, LEG, TREATMENT effectiveness, SURGICAL dressings, BANDAGES & bandaging, LONGITUDINAL method, ADULTS, OLD age
Abstract

Chronic wounds are a challenging medical entity for patients, medical professionals and healthcare systems. Frequently, patients present themselves to wound specialists after months or even years of unsuccessful treatment. Recent developments have resulted in a multitude of different advanced wound dressings created to treat complex, chronic wounds, one of which is the polylactide dressing Suprathel. This study aimed at investigating the healing potential of Suprathel in chronic wounds and differentiating between old and "young", diabetic and non‐diabetic chronic wounds. A prospective, multicentric, non‐controlled intervention study was conducted, treating patients with chronic lower leg ulcers (>3 months) with Suprathel and assessing them weekly. Afterwards, a retrospective analysis was performed analysing the wound size initially, after 4 and after 8 weeks of treatment. Furthermore, a differentiation between diabetic and non‐diabetic, and chronic wounds older and younger than 12 months, was assessed. A significant reduction in wound size was observed in the study population after 8 weeks of treatment. The effect size in the diabetic wound and the old chronic wound group even reached more than one, with the other groups still showing a large effect of the intervention. This study shows that Suprathel is a valuable tool in the armamentarium of a wound specialist. Not only could we show a positive effect on chronic wounds, we could even demonstrate a significant wound size reduction in chronic wounds of old and young, as well as diabetic wounds, with the treatment of older chronic and diabetic wounds yielding an even larger effect size. Further randomised, controlled studies are necessary to show the full potential of advanced wound dressing materials in large patient cohorts. [ABSTRACT FROM AUTHOR]

Published
2022
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41. Mit Mathematik zur Defektdeckung - 62 Jahre Micrografting

Author

Nischwitz, Sebastian P., Luze, Hanna, Holzer, Judith C. J., and Kamolz, Lars-Peter

Subjects
ddc: 610, 610 Medical sciences, Medicine
Abstract

Das Mesh-Grafting, als Goldstandard der Defektdeckung kleiner und mittelgroßer Areale, stößt trotz großer Effektivität bei großflächigen Verbrennungen an seine Grenzen. Die theoretisch postulierten Vergrößerungen konnten in der Praxis nachweislich nicht erreicht[zum vollständigen Text gelangen Sie über die oben angegebene URL], 38. Jahrestagung der Deutschsprachigen Arbeitsgemeinschaft für Verbrennungsbehandlung (DAV 2020)

Published
2020
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50. 615 Bacterial Nanocellulose as Cooling Agent

Author

Luze, Hanna, primary, Holzer, Judith, primary, Tiffner, Katrin, primary, Kainz, Sonja, primary, Reisenegger, Peter, primary, Nischwitz, Sebastian P, primary, Funk, Martin, primary, Birngruber, Thomas, primary, Mautner, Selma, primary, and Kamolz, Lars-Peter, primary

Published
2020
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