71 results on '"Luti, S."'
Search Results
2. XX Convegno Nazionale Società Italiana di Patologia Vegetale (S.I.Pa. V.): Environmentally loyal plant protection: from nano- to field-scale
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Luti, S., Baccelli, I., Lombardi, L., Martellini, F., Bernardi, R., Picciarelli, P., Scala, A., Pazzagli, L., Collinge, David B., Jensen, D.F., Dubey, M., Samils, N., Kosawang, C., Karlsson, M., Mannella, L., Turrà, D., Ghalid, M. El, Di Pietro, A., Boscia, D., Bianco, P.A., Vercesi, A., Piattella, M., Trematerra, P., Porceddu, E., Dusunceli, F., and Allara, M.
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- 2014
3. Honey extracts inhibit PTP1B, upregulate insulin receptor expression, and enhance glucose uptake in human HepG2 cells
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Lori, G., Cecchi, L., Mulinacci, N., Melani, F., Caselli, A., Cirri, P., Pazzagli, L., Luti, S., Mazzoli, L., and Paoli, P.
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- 2019
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4. Partitioning the structural features that underlie expansin-like and elicitor activities of cerato-platanin
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Luti, S., primary, Bemporad, F., additional, Vivoli Vega, M., additional, Leri, M., additional, Musiani, F., additional, Baccelli, I., additional, and Pazzagli, L., additional
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- 2020
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5. A rapid screening in oleuropein content and VOCs emission in fifteen olive cultivar leaves
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Colzi, I., Luti, S., Taiti, C., Marone, E., Masi, E., Pazzagli, L., Fiorino, P., and Mancuso, S.
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Olea europea ,Olea europea, cultivar, HPLC, PTR-ToF-MS ,HPLC ,oleuropein, VOCs, EVOO ,cultivar ,PTR-ToF-MS - Published
- 2018
6. Biological role in fungal life and molecular mechanism of action: two intriguing topics for the fungal PAMP cerato-platanin
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Baccelli, I., Luti, S., Martellini, F., Bernardi, Rodolfo, Pazzagli, L., and Scala, A.
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- 2013
7. The fungal MAMP cerato-platanin triggers resistance against Botrytis cinerea in Arabidopsis leaves
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Baccelli, I, Luti, S., Lombardi, Lara, Bernardi, Rodolfo, Picciarelli, Piero, Faoro, F., Pazzagli, L., and Scala, A.
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- 2013
8. Cerato-platanin and cerato-populin induce mapk activation in plane and Arabidopsis leaves
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Pazzagli, L., Luti, S., Lombardi, Lara, Baccelli, I., Bernardi, Rodolfo, Picciarelli, Piero, Martellini, F., and Scala, S.
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- 2012
9. Cerato-platanin and cerato-populin induce defence responses in plane leaves
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Martellini, F, Lombardi, Lara, Baccelli, I, Bernardi, Rodolfo, Picciarelli, Piero, Luti, S, Scala, A, and Pazzagli, L.
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- 2011
10. Can adaptation to metalliferous environments affect plant response to biotic stress? Insight from Silene paradoxa L. and phytoalexins
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Martellini, F., primary, Giorni, E., additional, Colzi, I., additional, Luti, S., additional, Meerts, P., additional, Pazzagli, L., additional, and Gonnelli, C., additional
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- 2014
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11. An interactive toolbox for atlas-based segmentation and coding of volumetric images
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Menegaz, G., primary, Luti, S., additional, Duay, V., additional, and Thiran, J.-Ph., additional
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- 2007
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12. An interactive toolbox for atlas-based segmentation and coding of volumetric images.
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Menegaz, G., Luti, S., Duay, V., and Thiran, J.-Ph.
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- 2007
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13. Philaenus italosignus a potential vector of Xylella fastidiosa: Occurrence of the spittlebug on olive trees in tuscany (Italy)
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Panzavolta, T., Bracalini, M., Croci, F., LUISA GHELARDINI, Luti, S., Campigli, S., Goti, E., Marchi, R., Tiberi, R., and Marchi, G.
14. Peptides obtained by targeted modifications of a natural Trichoderma peptaibol protect plants from grey mold disease and downy mildew
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LUCA SELLA, Marta De Zotti, ANGELA BOLZONELLO, Silvio Tundo, Luti, S., Bernardi, R., FRANCESCO FAVARON, and Baccelli, I.
15. Active contours and information theory for supervised segmentation on scalar images
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Duay, V., Luti, S., Gloria Menegaz, and Thiran, J. -P
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LTS5 - Abstract
Publication in the conference proceedings of EUSIPCO, Poznan, Poland, 2007
16. Model-based segmentation and coding of multidimensional medical images
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Luti, S., Duay, V., Menegaz, G., and Thiran, J.
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LTS5 ,LTS1
17. THE FUNGAL MAMP CERATO-PLATANIN TRIGGERS RESISTANCE AGAINST BOTRYTIS CINEREA IN ARABIDOPSIS LEAVES.
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Baccelli, I., Luti, S., Lombardi, L., Bernardi, R., Picciarelli, P., Faoro, F., Pazzagli, L., and Scala, A.
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DISEASE resistance of plants ,BOTRYTIS cinerea - Abstract
An abstract on the study relating to the benefits of fungal Mamp Cerato-Platanin in the Arabidopsis plant resistance against Botrytis Cinerea is presented.
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- 2013
18. CERATO-PLATANIN SHOWS EXPANSIN-LIKE ACTIVITY ON CELLULOSIC MATERIALS.
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Baccelli, I., Luti, S., Bernardi, R., Pazzagli, L., and Scala, A.
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CERATOCYSTIS diseases ,CANKER (Plant disease) ,PHYTOPATHOGENIC microorganisms ,FUNGAL diseases of plants ,GENE expression ,COTTON fibers ,CELLULASE - Abstract
Cerato-platanin (CP) is a protein elicitor produced by Ceratocystis platani, the causal agent of canker stain of plane tree. The eliciting activity of CP has been extensively studied, and in accordance with the "zig-zag model" described by Jones and Dangl (2006), CP may be considered a PAMP (pathogen-associated molecular pattern). However, the primary role of CP and of all the other proteins of the "cerato-platanin family" in fungi is unknown. CP is both a structural component of the cell wall of C. platani and a protein secreted in the medium where the fungus grows. No lytic activity has ever been reported. Recently, by studying the three-dimensional structure of the protein and the pattern of gene expression, a clue on its role has been found: CP could act in the remodelling and enlargement of the cell wall during hyphal growth and in the formation of chlamydospores, with a role like that of expansins. In the present work, the expansinlike activity of CP was investigated. Like expansins, CP was able to disrupt filter paper in a concentration-dependent manner and to cause fragmentation of the crystalline cellulose Avicel. Cotton fibers incubated for 24 h with CP, then sonicated, showed breakage and defibration, whereas fibers incubated in buffer alone remained undamaged. Optimum of pH and temperature for CP activity were determined. Results strongly suggest that CP may be a new fungal expansin. Its possible synergistic effect with cellulases is under investigation. [ABSTRACT FROM AUTHOR]
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- 2012
19. Follicular microenvironment: Oxidative stress and adiponectin correlated with steroids hormones in women undergoing in vitro fertilization
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Tania Fiaschi, Tania Gamberi, Giuseppe Morgante, Gabriella Pinto, Angela Amoresano, Pietro Amedeo Modesti, Bianca Semplici, Francesca Magherini, Alessandra Modesti, Anna Illiano, Paola Piomboni, Simone Luti, Luti, S., Fiaschi, T., Magherini, F., Modesti, P. A., Piomboni, P., Semplici, B., Morgante, G., Amoresano, A., Illiano, A., Pinto, G., Modesti, A., and Gamberi, T.
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human assisted reproduction ,Adult ,0301 basic medicine ,endocrine system ,medicine.medical_specialty ,women fertility ,Adipokine ,Inflammation ,Fertilization in Vitro ,Biology ,medicine.disease_cause ,03 medical and health sciences ,0302 clinical medicine ,Ovarian Follicle ,Internal medicine ,Follicular phase ,Genetics ,medicine ,Humans ,Steroid ,reactive oxygen specie ,Cumulus Cell ,Cumulus Cells ,030219 obstetrics & reproductive medicine ,Adiponectin ,Ovary ,Oxidative Stre ,Cell Biology ,Follicular fluid ,Follicular Fluid ,Oxidative Stress ,030104 developmental biology ,Endocrinology ,Cellular Microenvironment ,steroid hormone ,Theca ,Female ,Steroids ,medicine.symptom ,Oxidative stress ,Human ,Developmental Biology ,Hormone - Abstract
Research has been focused on determining the follicular microenviroment produced by the theca and granulosa cells since the molecular characterisation of this body fluid could lead to the understanding of several fertility problems. Oxidative stress may be one of the factors involved in female infertility since it plays a key role in the modulation of oocyte maturation and finally pregnancy. An increase in oxidative stress is correlated with inflammation and intense research was developed to understand the interaction between inflammation and adiponectin, based on the fact that many adipokines are inflammation related proteins linked to reactive oxygen species production. The aim of this study is to investigate the correlation between total adiponectin levels and oxidative stress amount in the serum and follicular fluid (FF) of women who undergone in vitro fertilization. Moreover we verified the expression of adiponectin in granulosa and cumulus cells. To clarify the predictive value of steroid hormones in human assisted reproduction, twelve steroid hormones in FF and serum, were quantified in a single run liquid chromatography/mass spectrometry, by using a multiple reaction monitoring mode and we related the serum and follicular fluids adiponectin levels with the concentration of the investigated steroid hormones.
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- 2020
20. Relationships between Sex and Adaptation to Physical Exercise in Young Athletes: A Pilot Study
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Gabriella Pinto, Rosamaria Militello, Angela Amoresano, Pietro Amedeo Modesti, Alessandra Modesti, Simone Luti, Pinto, G., Militello, R., Amoresano, A., Modesti, P. A., Modesti, A., and Luti, S.
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Health Information Management ,Leadership and Management ,sport metabolomics ,oxidative stress ,hormone signalling ,adiponectin ,basketball ,Health Policy ,Sport metabolomics ,Oxidative stre ,Health Informatics ,Adiponectin ,Basketball ,Hormone signalling ,Oxidative stress - Abstract
The purpose of this study was to compare the redox, hormonal, metabolic, and lipid profiles of female and male basketball players during the seasonal training period, compared to their relative sedentary controls. 20 basketball players (10 female and 10 male) and 20 sedentary controls (10 female and 10 male) were enrolled in the study. Oxidative stress, adiponectin level, and metabolic profile were determined. Male and female athletes showed an increased antioxidant capacity (27% for males; 21% for females) and lactate level (389% for males; 460% for females) and reduced salivary cortisol (25% for males; 51% for females) compared to the sedentary controls. Moreover, a peculiar metabolite (in particular, amino acids and urea), hormonal, and lipidic profile were highlighted in the two groups of athletes. Female and male adaptations to training have several common traits, such as antioxidant potential enhancement, lactate increase, and activation of detoxifying processes, such as the urea cycle and arachidonic pathways as a response to inflammation. Moreover, we found different lipid and amino acid utilization related to sex. Deeper investigation could help coaches in developing training programs based on the athletes’ sex in order to reduce the drop-out rate of sporting activity by girls and fight the gender stereotypes in sport that also have repercussions in social fields.
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- 2022
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21. Partitioning the structural features that underlie expansin-like and elicitor activities of cerato-platanin
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Ivan Baccelli, Manuela Leri, Luigia Pazzagli, S. Luti, M. Vivoli Vega, Francesco Musiani, Francesco Bemporad, Luti S., Bemporad F., Vivoli Vega M., Leri M., Musiani F., Baccelli I., and Pazzagli L.
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Models, Molecular ,Protein Folding ,PAMPs ,Structural similarity ,Protein Conformation ,Mutant ,Cerato-platanin Expansin-like activity PAMPs Cellulose-binding E. coli SHuffle ,02 engineering and technology ,Biochemistry ,E. coli SHuffle ,Fungal Proteins ,03 medical and health sciences ,Expansin ,chemistry.chemical_compound ,Structural Biology ,Cerato-plataninExpansin-like activityPAMPsCellulose-bindingE. coli SHuffle ,Cerato-platanin ,Cellulose ,Molecular Biology ,030304 developmental biology ,0303 health sciences ,Chemistry ,Protein Stability ,Fungi ,General Medicine ,021001 nanoscience & nanotechnology ,Cellulose binding ,Elicitor ,Cell biology ,Molecular Docking Simulation ,Structural Homology, Protein ,Expansin-like activity ,Mutation ,Mutagenesis, Site-Directed ,Cellulose-binding ,Conformational stability ,0210 nano-technology - Abstract
Cerato-platanin family (CPF) proteins are produced by fungi and elicit defences when applied to plants, behaving as PAMPs/MAMPs. CPF proteins share structural similarity to plant and bacterial expansins, and have been demonstrated, in some cases, to possess expansin-like loosening activity on cellulose. This is the case of cerato-platanin (CP), the founder of the CPF, which shows both eliciting and cellulose-loosening activities, raising the question as to whether the expansin-like activity may be responsible for defence activation. To pinpoint structural and thermodynamic features underlying eliciting and expansin-like activity of CP, we carried out site-directed mutagenesis targeting separately net charge (N84D mutation), conformational stability (V63A mutation), or conserved position previously shown to affect expansin-like activity in CP (D77A mutation), and characterized wild-type protein and its variants. Removing or adding negative charges on the protein surface led to reducing or increasing, respectively, the expansin-like activity. The activity was instead not affected by mutations affecting protein fold and stability. In contrast, all the mutants showed reduced capacity to elicit defences in plants. We conclude that the expansin-like activity of CP depends on net charge and ability to (weakly) bind cellulose, whereas the eliciting activity on plants does not depend on the cellulose-loosening activity.
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- 2020
22. Relationship between the metabolic and lipid profile in follicular fluid of women undergoing in vitro fertilization
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Tania Fiaschi, Gabriella Pinto, Angela Amoresano, Paola Piomboni, Alessandra Modesti, Laura Governini, Francesca Magherini, Simone Luti, Pietro Amedeo Modesti, Alice Luddi, Tania Gamberi, Anna Illiano, Luti, S., Fiaschi, T., Magherini, F., Modesti, P. A., Piomboni, P., Governini, L., Luddi, A., Amoresano, A., Illiano, A., Pinto, G., Modesti, A., and Gamberi, T.
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0301 basic medicine ,Oocyte ,medicine.disease_cause ,0302 clinical medicine ,Ovarian Follicle ,Follicular phase ,lipidic profile ,030219 obstetrics & reproductive medicine ,medicine.diagnostic_test ,Lipid ,Lipids ,metabolic profile ,Cellular Microenvironment ,Metabolome ,network pathways ,Female ,Infertility, Female ,Metabolic Networks and Pathways ,follicular microenvironment ,Human ,Infertility ,Adult ,medicine.medical_specialty ,Metabolomic ,Fertilization in Vitro ,Biology ,03 medical and health sciences ,Metabolomics ,network pathway ,Internal medicine ,Genetics ,medicine ,Humans ,Adiponectin ,Metabolic Networks and Pathway ,Oxidative Stre ,Cell Biology ,medicine.disease ,Lipid Metabolism ,Follicular fluid ,Follicular Fluid ,Oxidative Stress ,030104 developmental biology ,Endocrinology ,Oocytes ,Lipid profile ,Oxidative stress ,Developmental Biology ,Hormone - Abstract
Among the follicular fluid (FF) components promoting the development of the oocyte are included glycoproteins, several fatty acids, and steroid hormones synthesized by the dominant follicle. For this, the analysis of the metabolites present in FF can determine the quality of the oocyte. FF composition is in part determined by local follicular metabolic processes and in part a plasma transudate. Since the causes of impaired fertility may be due to a metabolic imbalance, metabolomics is useful to identify low molecular weight metabolites. Oxidative stress is involved in human infertility and the use of metabolomics can be crucial to identify which other metabolites besides reactive oxygen species are involved in oxidative stress correlated to infertility. To obtain new information on the study of signaling molecules in FF, the knowledge of the lipid content will be important to improve information on the understanding of follicular development. The objective of this study is to identify (a) a metabolic profile and a lipid profile of FF in women undergoing in vitro fertilization and (b) to correlate the previous information obtained regarding adiponectin and oxidative stress with the metabolic and lipid profile obtained in the present study. As result, we found an increase in oxidative stress due to both an increase of androgens and an accumulation of lipids in the follicular environment and we suggest that this might be one of the causes of reduced fertility.
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- 2020
23. Reprogramming of Amino Acid Transporters to Support Aspartate and Glutamate Dependency Sustains Endocrine Resistance in Breast Cancer
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Bruno M Simões, Andrea Morandi, Giuseppina Comito, Marina Bacci, Simone Romagnoli, Elisabetta Marangoni, Paola Chiarugi, Massimiliano Mazzone, Matteo Ramazzotti, Nicla Lorito, Manuela Ferracin, Simone Luti, Lesley-Ann Martin, Francesca Bianchini, Qiong Gao, Federico Virga, Federica Cappellesso, Matteo Parri, Elisa Giannoni, Luigi Ippolito, Bacci M., Lorito N., Ippolito L., Ramazzotti M., Luti S., Romagnoli S., Parri M., Bianchini F., Cappellesso F., Virga F., Gao Q., Simoes B.M., Marangoni E., Martin L.-A., Comito G., Ferracin M., Giannoni E., Mazzone M., Chiarugi P., and Morandi A.
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0301 basic medicine ,Amino Acid Transport Systems ,Neutral ,Drug Resistance ,Estrogen receptor ,SLCs ,THERAPY ,amino acid transporter ,Mice ,0302 clinical medicine ,metabolic reprogramming ,Neoplasm Metastasis ,amino acid transporters ,aspartate ,endocrine therapy ,estrogen receptor ,glutamate ,miRNA ,resistance ,lcsh:QH301-705.5 ,Inbred BALB C ,chemistry.chemical_classification ,Heterologous ,Mice, Inbred BALB C ,Tumor ,biology ,Manchester Cancer Research Centre ,Chemistry ,SIGNATURE ,Glutamate receptor ,SLC1A2 ,Prognosis ,3. Good health ,Amino acid ,Amino Acid Transport Systems, Neutral ,Animals ,Aspartic Acid ,Breast Neoplasms ,Cell Line, Tumor ,Cell Survival ,Drug Resistance, Neoplasm ,Estrogen Receptor alpha ,Excitatory Amino Acid Transporter 2 ,Female ,GATA2 Transcription Factor ,Gas Chromatography-Mass Spectrometry ,Gene Expression Regulation, Neoplastic ,Glutamic Acid ,Humans ,MicroRNAs ,Transcriptome ,Transplantation, Heterologous ,TARGET ,SURVIVAL ,AUTOPHAGY ,Life Sciences & Biomedicine ,EXPRESSION DATA ,General Biochemistry, Genetics and Molecular Biology ,Article ,Cell Line ,03 medical and health sciences ,Downregulation and upregulation ,Amino acid transporter ,TAMOXIFEN ,Neoplastic ,Transplantation ,Science & Technology ,RECEPTOR ,ResearchInstitutes_Networks_Beacons/mcrc ,Autophagy ,Transporter ,Cell Biology ,030104 developmental biology ,Gene Expression Regulation ,lcsh:Biology (General) ,CELLS ,METASTASIS ,biology.protein ,Cancer research ,Neoplasm ,030217 neurology & neurosurgery - Abstract
Summary Endocrine therapy (ET) is the standard of care for estrogen receptor-positive (ER+) breast cancers. Despite its efficacy, ∼40% of women relapse with ET-resistant (ETR) disease. A global transcription analysis in ETR cells reveals a downregulation of the neutral and basic amino acid transporter SLC6A14 governed by enhanced miR-23b-3p expression, resulting in impaired amino acid metabolism. This altered amino acid metabolism in ETR cells is supported by the activation of autophagy and the enhanced import of acidic amino acids (aspartate and glutamate) mediated by the SLC1A2 transporter. The clinical significance of these findings is validated by multiple orthogonal approaches in a large cohort of ET-treated patients, in patient-derived xenografts, and in in vivo experiments. Targeting these amino acid metabolic dependencies resensitizes ETR cells to therapy and impairs the aggressive features of ETR cells, offering predictive biomarkers and potential targetable pathways to be exploited to combat or delay ETR in ER+ breast cancers., Graphical Abstract, Highlights • ETR cells show high miR-23b-3p that reduces SLC6A14 and amino acids upload • ETR cells promote autophagy and aspartate and glutamate import via SLC1A2 • Aspartate and glutamate fuel anabolic and catabolic pathways in ETR breast cancers • Targeting amino acid metabolic reprogramming is effective in ETR cells, Bacci et al. find that endocrine-resistant ER+ breast cancers are characterized by enhanced miR-23b-3p, autophagy activation, and import of aspartate and glutamate that fuel catabolic and anabolic pathways, which are essential for their aggressive features. The molecular players involved in this metabolic scenario are of clinical significance and have prognostic and predictive value.
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- 2019
24. S-Homocysteinylation effects on transthyretin: worsening of cardiomyopathy onset
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Monica Bucciantini, Anna Caselli, Sofia Giorgetti, Paolo De Paoli, Manuela Leri, Massimo Stefani, Paola Rebuzzini, Loredana Marchese, Silvia Garagna, Simone Luti, Antonino Natalello, Leri, M, Rebuzzini, P, Caselli, A, Luti, S, Natalello, A, Giorgetti, S, Marchese, L, Garagna, S, Stefani, M, Paoli, P, and Bucciantini, M
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Amyloid disease ,0301 basic medicine ,endocrine system ,medicine.medical_specialty ,Homocysteine ,Protein Conformation ,Mutant ,Biophysics ,Cardiomyopathy ,FIS/07 - FISICA APPLICATA (A BENI CULTURALI, AMBIENTALI, BIOLOGIA E MEDICINA) ,medicine.disease_cause ,Biochemistry ,L55P-TTR ,Structure-Activity Relationship ,03 medical and health sciences ,chemistry.chemical_compound ,Methionine ,Internal medicine ,medicine ,Humans ,Prealbumin ,Myocytes, Cardiac ,FAC ,Molecular Biology ,Amyloid Neuropathies, Familial ,Mutation ,030102 biochemistry & molecular biology ,biology ,Protein Stability ,Chemistry ,Cardiomiopathy Amyloid disease FAP FAC L55P-TTR Homocysteine ,Cardiac muscle ,FAP ,nutritional and metabolic diseases ,medicine.disease ,Cardiomiopathy ,Stroke ,Transthyretin ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,Diabetes Mellitus, Type 2 ,Cardiovascular Diseases ,biology.protein ,Cardiomyopathies - Abstract
Background L-Homocysteine (Hcy) is a non-proteinogenic α-amino acid synthesized from dietary methionine. In healthy humans, high Hcy levels are a risk factor for cardiovascular diseases, stroke and type 2 diabetes. A recent study reports that Hcy reacts with Cys10 of transthyretin (TTR), generating a stable covalent adduct. However, to date the effect of S-homocysteinylation on TTR conformational stability remains unknown. Methods The effect of Hcy on the conformational properties of wt- and L55P-TTR were analysed using a set of biophysical techniques. The cytotoxicity of S-homocysteinylated L55P-TTR was also evaluated in the HL-1 cardiomyocyte cell line, while the effects of the assemblies on kinematic and dynamics properties of cardiac muscle cells were analysed in cardiomyocyte syncytia. Results We found that Hcy stabilizes tetrameric wt-TTR, while it destabilizes the tetrameric structure of the L55P mutant, promoting the accumulation of self-assembly-prone monomeric species. Conclusions Our study demonstrated that S-homocysteinylation of the L55P-TTR mutant impairs protein stability, favouring the appearance of toxic monomers. Interestingly, S-homocysteinylation affected only mutant, not wt-TTR. Moreover, we also show that assemblies of S-homocysteinylated L55P-TTR impair cardiomyocytes functional parameters. General significance Our study offers new insights on the negative impact of S-homocysteinylation on L55P-TTR stability, whose aggregation is considered the causative agent of a form of early-onset familial amyloid polyneuropathy and cardiomyopathy. Our results suggest that high homocysteine levels are a further risk factor for TTR cardiomyopathy in patients harbouring the L55P-TTR mutation.
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- 2020
25. Editorial: Metabolic responses and adaptations to exercise.
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Gamberi T, Vassalle C, Nikolaidis PT, Pellegrino A, and Luti S
- Abstract
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision.
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- 2024
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26. Physical Activity and Oxidative Stress in Aging.
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Militello R, Luti S, Gamberi T, Pellegrino A, Modesti A, and Modesti PA
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Biological aging, characterized by changes in metabolism and physicochemical properties of cells, has an impact on public health. Environment and lifestyle, including factors like diet and physical activity, seem to play a key role in healthy aging. Several studies have shown that regular physical activity can enhance antioxidant defense mechanisms, including the activity of enzymes such as superoxide dismutase (SOD), catalase, and glutathione peroxidase. However, intense or prolonged exercise can also lead to an increase in reactive oxygen species (ROS) production temporarily, resulting in oxidative stress. This phenomenon is referred to as "exercise-induced oxidative stress". The relationship between physical activity and oxidative stress in aging is complex and depends on various factors such as the type, intensity, duration, and frequency of exercise, as well as individual differences in antioxidant capacity and adaptation to exercise. In this review, we analyzed what is reported by several authors regarding the role of physical activity on oxidative stress in the aging process as well as the role of hormesis and physical exercise as tools for the prevention and treatment of sarcopenia, an aging-related disease. Finally, we reported what has recently been studied in relation to the effect of physical activity and sport on aging in women.
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- 2024
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27. Multiple lysine substitutions in the peptaibol trichogin GA IV enhance the antibiotic activity against plant pathogenic Pseudomonas syringae.
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Fodil S, De Zotti M, Tundo S, Gabbatore L, Vettorazzo I, Luti S, Musetti R, Sella L, Favaron F, and Baccelli I
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- Plant Diseases microbiology, Plant Diseases prevention & control, Peptaibols pharmacology, Peptaibols chemistry, Microbial Sensitivity Tests, Oligopeptides pharmacology, Oligopeptides chemistry, Solanum lycopersicum microbiology, Pseudomonas syringae drug effects, Lysine chemistry, Lysine pharmacology, Anti-Bacterial Agents pharmacology
- Abstract
Plant diseases caused by Pseudomonas syringae are essentially controlled in the field with the use of copper-based products and antibiotics, raising environmental and safety concerns. Antimicrobial peptides (AMPs) derived from fungi may represent a sustainable alternative to those chemicals. Trichogin GA IV, a non-ribosomal, 11-residue long AMP naturally produced by the fungus Trichoderma longibrachiatum has the ability to insert into phospholipidic membranes and form water-filled pores, thereby perturbing membrane integrity and permeability. In previous studies, peptide analogs modified at the level of specific residues were designed to be water-soluble and active against plant pathogens. Here, we studied the role of glycine-to-lysine substitutions and of the presence of a C-terminal leucine amide on bioactivity against Pseudomonas syringae bacteria. P. syringae diseases affect a wide range of crops worldwide, including tomato and kiwifruit. Our results show that trichogin GA IV analogs containing two or three Gly-to-Lys substitutions are highly effective in vitro against P. syringae pv. tomato (Pst), displaying minimal inhibitory and minimal bactericidal concentrations in the low micromolar range. The same analogs are also able to inhibit in vitro the kiwifruit pathogen P. syringae pv. actinidiae (Psa) biovar 3. When sprayed on tomato plants 24 h before Pst inoculation, only tri-lysine containing analogs were able to significantly reduce bacterial titers and symptom development in infected plants. Our results point to a positive correlation between the number of lysine substitutions and the antibacterial activity. This correlation was supported by microscopy analyses performed with mono-, di- and tri-Lys containing analogs that showed a different degree of interaction with Pst cells and ultrastructural changes that culminated in cell lysis., Competing Interests: Declaration of competing interest The authors declare the absence of any conflict of interest., (Copyright © 2023. Published by Elsevier Inc.)
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- 2024
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28. Chronic lactate exposure promotes cardiomyocyte cytoskeleton remodelling.
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Luti S, Militello R, Pinto G, Illiano A, Marzocchini R, Santi A, Becatti M, Amoresano A, Gamberi T, Pellegrino A, Modesti A, and Modesti PA
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We investigated the effect of growing on lactate instead of glucose in human cardiomyocyte assessing their viability, cell cycle activity, oxidative stress and metabolism by a proteomic and metabolomic approach. In previous studies performed on elite players, we found that adaptation to exercise is characterized by a chronic high plasma level of lactate. Lactate is considered not only an energy source but also a signalling molecule and is referred as "lactormone"; heart is one of the major recipients of exogenous lactate. With this in mind, we used a cardiac cell line AC16 to characterize the lactate metabolic profile and investigate the metabolic flexibility of the heart. Interestingly, our data indicated that cardiomyocytes grown on lactate (72 h) show change in several proteins and metabolites linked to cell hypertrophy and cytoskeleton remodelling. The obtained results could help to understand the effect of this metabolite on heart of high-performance athletes., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Authors.)
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- 2024
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29. Effect of Bovine Milk Peptides on Cell Inflammation, Proliferation and Differentiation: Milk Potential Benefits Are Preserved in an Unconventional Cow Feeding Strategy.
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Cicchi C, Paoli P, Modesti A, Mannelli F, Scicutella F, Buccioni A, Fontanarosa C, Luti S, and Pazzagli L
- Abstract
Animal feeding through the reuse of agro-industrial by-products in one of the ultimate goals of sustainable agriculture. Olive oil pomace (OOP) produced as a waste product during olive oil milling has been used as an ingredient in the diet for Holstein lactating cows. Recent findings have shown no decrease in animal performance, feed intake or detrimental effect on rumen microbiota. In contrast, an improvement in C18 polyunsaturated fatty acids has been observed. In this work, the milk protein content from cows fed a commercial diet (CON) or an experimental one supplemented with OOP was determined and compared, and the peptides derived from the simulated gastrointestinal digestion of raw milk were analyzed. After fractionation via RP-HPLC, peptides were characterized for their biological activity on different cell lines. The ability to reduce both the intracellular ROS content and the expression of inflammatory markers, such as Cyclooxygenase, isoenzyme 2 (COX-2) and inducible Nitric Oxide Synthase (iNOS), as well as the remarkable properties to induce cell differentiation and to slow down the proliferation of human intestinal cancer cells, enable us to define them as bioactive peptides. In spite of there being no observed significant difference between the healthy activity of CON and OOP peptides, the results allow us to broaden the knowledge about the biological activity of these bioactive peptides and to confirm that agro-industrial by-products may be successfully incorporated into the feeding strategy of dairy cows.
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- 2023
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30. Metabolic Responses in Leaves of 15 Italian Olive Cultivars in Correspondence to Variable Climatic Elements.
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Colzi I, Marone E, Luti S, Pazzagli L, Mancuso S, and Taiti C
- Abstract
This study aims to evaluate the metabolic changes that occurred in olive leaves as responses over time to variations in climatic elements. Rainfall, temperature, and solar radiation data were collected over 4 months (August-November) to assess the impact of different climatic trends on the metabolism of the leaves of 15 Italian olive cultivars, cultivated at the experimental farm of the University of Florence. The net photosynthetic rate (A
N ) and stomatal conductance (gs ), measured as main indicators of primary metabolism, were mainly influenced by the "cultivar" effect compared to the "climate" effect. The lowest AN value was showed by "Bianchera", while "Ascolana" recorded the highest (8.6 and 13.6 µmol CO2 m-2 s-1 , respectively). On the other hand, the secondary metabolism indicators, volatile organic compound (VOC) and oleuropein (OL) content, were much more influenced by climate trends, especially rainfall. A phase of high rainfall caused a significant increase in the VOCs emission from leaves, even with different behaviors among the genotypes. The highest differences were observed between "Maiatica di Ferrandina", with the highest average values (~85,000 npcs), and "Frantoio", which showed the lowest (~22,700 npcs). The OL content underwent considerable fluctuations in relation to the rainfall but also appeared to be controlled by the genotype. "Coratina" always showed the highest OL concentration (reaching the maximum ~98 mg g-1 ), indicating the great potential of this cultivar for the industrial recovery of OL.- Published
- 2023
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31. Designed multiple ligands for the treatment of type 2 diabetes mellitus and its complications: Discovery of (5-arylidene-4-oxo-2-thioxothiazolidin-3-yl)alkanoic acids active as novel dual-targeted PTP1B/AKR1B1 inhibitors.
- Author
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Maccari R, Wolber G, Genovese M, Sardelli G, Talagayev V, Balestri F, Luti S, Santi A, Moschini R, Del Corso A, Paoli P, and Ottanà R
- Subjects
- Humans, Phosphoric Monoester Hydrolases, Ligands, Enzyme Inhibitors pharmacology, Enzyme Inhibitors therapeutic use, Protein Tyrosine Phosphatase, Non-Receptor Type 1, Aldehyde Reductase, Diabetes Mellitus, Type 2 drug therapy
- Abstract
Type 2 diabetes mellitus (T2DM) is a serious chronic disease with an alarmingly growing worldwide prevalence. Current treatment of T2DM mainly relies on drug combinations in order to control blood glucose levels and consequently prevent the onset of hyperglycaemia-related complications. The development of multiple-targeted drugs recently emerged as an attractive alternative to drug combinations for the treatment of complex diseases with multifactorial pathogenesis, such as T2DM. Protein tyrosine phosphatase 1B (PTP1B) and aldose reductase (AKR1B1) are two enzymes crucially involved in the development of T2DM and its chronic complications and, therefore, dual inhibitors targeted to both these enzymes could provide novel agents for the treatment of this complex pathological condition. In continuing our search for dual-targeted PTP1B/AKR1B1 inhibitors, we designed new (5-arylidene-4-oxo-2-thioxothiazolidin-3-yl)alkanoic acids. Among them, 3-(4-phenylbutoxy)benzylidene derivatives 6f and 7f, endowed with interesting inhibitory activity against both targets, proved to control specific cellular pathways implicated in the development of T2DM and related complications., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Masson SAS. All rights reserved.)
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- 2023
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32. Plant Defense Elicitation by the Hydrophobin Cerato-Ulmin and Correlation with Its Structural Features.
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Gallo M, Luti S, Baroni F, Baccelli I, Cilli EM, Cicchi C, Leri M, Spisni A, Pertinhez TA, and Pazzagli L
- Subjects
- Fungal Proteins metabolism, Fungi metabolism, Wettability, Hydrophobic and Hydrophilic Interactions, Plumbaginaceae metabolism
- Abstract
Cerato-ulmin (CU) is a 75-amino-acid-long protein that belongs to the hydrophobin family. It self-assembles at hydrophobic-hydrophilic interfaces, forming films that reverse the wettability properties of the bound surface: a capability that may confer selective advantages to the fungus in colonizing and infecting elm trees. Here, we show for the first time that CU can elicit a defense reaction (induction of phytoalexin synthesis and ROS production) in non-host plants ( Arabidopsis ) and exerts its eliciting capacity more efficiently when in its soluble monomeric form. We identified two hydrophobic clusters on the protein's loops endowed with dynamical and physical properties compatible with the possibility of reversibly interconverting between a disordered conformation and a β-strand-rich conformation when interacting with hydrophilic or hydrophobic surfaces. We propose that the plasticity of those loops may be part of the molecular mechanism that governs the protein defense elicitation capability.
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- 2023
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33. Chronic Training Induces Metabolic and Proteomic Response in Male and Female Basketball Players: Salivary Modifications during In-Season Training Programs.
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Luti S, Militello R, Pinto G, Illiano A, Amoresano A, Chiappetta G, Marzocchini R, Modesti PA, Pratesi S, Pazzagli L, Modesti A, and Gamberi T
- Abstract
The aim of this study was to characterize the salivary proteome and metabolome of highly trained female and male young basketball players, highlighting common and different traits. A total of 20 male and female basketball players (10 female and 10 male) and 20 sedentary control subjects (10 female and 10 male) were included in the study. The athletes exercised at least five times per week for 2 h per day. Saliva samples were collected mid-season, between 9:00 and 11:00 a.m. and away from sport competition. The proteome and metabolome were analyzed by using 2DE and GC-MS techniques, respectively. A computerized 2DE gel image analysis revealed 43 spots that varied in intensity among groups. Between these spots, 10 (23.2%) were differentially expressed among male athletes and controls, 22 (51.2%) between female basketball players and controls, 11 spots (25.6%) between male and female athletes, and 13 spots (30.2%) between male and female controls. Among the proteins identified were Immunoglobulin, Alpha-Amylase, and Dermcidin, which are inflammation-related proteins. In addition, several amino acids, such as glutamic acid, lysine, ornithine, glycine, tyrosine, threonine, and valine, were increased in trained athletes. In this study, we highlight that saliva is a useful biofluid to assess athlete performance and confirm that the adaptation of men and women to exercise has some common features, but also some different sex-specific behaviors, including differential amino acid utilization and expression of inflammation-related proteins, which need to be further investigated. Moreover, in the future, it will be interesting to examine the influence of sport-type on these differences., Competing Interests: The authors declare no conflicts of interest.
- Published
- 2023
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34. Preliminary results indicate that regular training induces high protection against oxidative stress in basketball players compared to soccer.
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Luti S, Militello R, Fiaschi T, Magherini F, Gamberi T, Parri M, Marzocchini R, Pratesi S, Soldaini R, Modesti A, and Modesti PA
- Subjects
- Humans, Male, Adiponectin, Athletes, Oxidative Stress, Basketball, Soccer
- Abstract
In elite athlete several metabolic changes occur during regular training. These modifications are associated with changes in blood metabolic profile and can lead to adaptive mechanisms aimed at establish a new dynamic equilibrium, which guarantees better performance. The goal of this study was to characterize the plasma metabolic profile and redox homeostasis, in athletes practicing two different team sports such as soccer and basketball in order to identify potential metabolic pathways underlying the differences in training programs. A cohort of 30 male, 20 professional players (10 soccer and 10 basketballs) and 10 sedentary males as control were enrolled in the study. Plasma redox balance, metabolites and adiponectin were determined. The results show low levels of oxidative species (25.5%), with both high antioxidant capacity (17.6%) and adiponectin level (64.4%) in plasma from basketball players, in comparison to soccer players. Metabolic analysis indicates in basketball players a significant high plasma level of amino acids Valine and Ornithine both involved in redox homeostasis and anti-inflammatory metabolism., (© 2022. The Author(s).)
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- 2022
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35. Editorial: Women and men in physical activity.
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Modesti A, Luti S, Pinto G, Vassalle C, and Nikolaidis PT
- Abstract
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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- 2022
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36. Differential impact of cold and hot tea extracts on tyrosine phosphatases regulating insulin receptor activity: a focus on PTP1B and LMW-PTP.
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Genovese M, Luti S, Pardella E, Vivoli-Vega M, Pazzagli L, Parri M, Caselli A, Cirri P, and Paoli P
- Subjects
- Enzyme Inhibitors chemistry, Enzyme Inhibitors pharmacology, Humans, Insulin metabolism, Plant Extracts chemistry, Plant Extracts pharmacology, Protein Tyrosine Phosphatase, Non-Receptor Type 1 metabolism, Signal Transduction, Insulin Resistance, Protein Tyrosine Phosphatases metabolism, Receptor, Insulin metabolism, Tea chemistry
- Abstract
Purpose: The impact of tea constituents on the insulin-signaling pathway as well as their antidiabetic activity are still debated questions. Previous studies suggested that some tea components act as Protein Tyrosine Phosphatase 1B (PTP1B) inhibitors. However, their nature and mechanism of action remain to be clarified. This study aims to evaluate the effects of both tea extracts and some of their constituents on two main negative regulators of the insulin-signaling pathway, Low-Molecular-Weight Protein Tyrosine Phosphatase (LMW-PTP) and PTP1B., Methods: The effects of cold and hot tea extracts on the enzyme activity were evaluated through in vitro assays. Active components were identified using gas chromatography-mass spectrometry (GC-MS) analysis. Finally, the impact of both whole tea extracts and specific active tea components on the insulin-signaling pathway was evaluated in liver and muscle cells., Results: We found that both cold and hot tea extracts inhibit LMW-PTP and PTP1B, even if with a different mechanism of action. We identified galloyl moiety-bearing catechins as the tea components responsible for this inhibition. Specifically, kinetic and docking analyses revealed that epigallocatechin gallate (EGCG) is a mixed-type non-competitive inhibitor of PTP1B, showing an IC
50 value in the nanomolar range. Finally, in vitro assays confirmed that EGCG acts as an insulin-sensitizing agent and that the chronic treatment of liver cells with tea extracts results in an enhancement of the insulin receptor levels and insulin sensitivity., Conclusion: Altogether, our data suggest that tea components are able to regulate both protein levels and activation status of the insulin receptor by modulating the activity of PTP1B., (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany.)- Published
- 2022
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37. Water-Soluble Trichogin GA IV-Derived Peptaibols Protect Tomato Plants From Botrytis cinerea Infection With Limited Impact on Plant Defenses.
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Baccelli I, Luti S, Bernardi R, Favaron F, De Zotti M, and Sella L
- Abstract
Peptaibols are non-ribosomal linear peptides naturally produced by a wide variety of fungi and represent the largest group of peptaibiotic molecules produced by Trichoderma species. Trichogin GA IV is an 11-residue lipo peptaibol naturally produced by Trichoderma longibrachiatum . Peptaibols possess the ability to form pores in lipid membranes or perturb their surface, and have been studied as antibiotics or anticancer drugs in human medicine, or as antimicrobial molecules against plant pathogens. When applied to plants, peptaibols may also elicit defense responses. A major drawback to the exploitation and application of peptaibols in agriculture is their poor water solubility. In a previous study, we designed water-soluble Lys-containing Trichogin GA IV analogs, which were able to inhibit the growth of several fungal plant pathogens in vitro . In the present study, we shed light on the mechanism underpinning their efficacy on plants, focusing on six Trichogin GA IV analogs. Our results highlighted peptide hydrophilicity, rather than helix stability, as the major determinant of their activity against B. cinerea infection in tomato leaves. The peptides showed preventive but not curative efficacy against infection, and lack of translaminar activity, with results reproducible on two tomato cultivars, Marmande and Micro-Tom. Reactive oxygen species (ROS) detection analysis in tomato and Arabidopsis , and expression of defense genes in tomato, highlighted a transient and limited impact of the peptides on the plant defense system. The treatment did not result in significant modulation of defense genes or defense priming. The antimicrobial effect thus emerges as the only mechanism behind the plant protection ability exerted by water-soluble Trichogin GA IV analogs, and limited effects on the plant metabolism are expected to occur., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Baccelli, Luti, Bernardi, Favaron, De Zotti and Sella.)
- Published
- 2022
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38. Modulation of Plasma Proteomic Profile by Regular Training in Male and Female Basketball Players: A Preliminary Study.
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Militello R, Pinto G, Illiano A, Luti S, Magherini F, Amoresano A, Modesti PA, and Modesti A
- Abstract
Monitoring fatigue and recovery during training periods contributes to identifying the best training methods to achieve sports performance. To date, little is known about sex-related differences in sports adaptations. The aim of the present study is to identify sex-related sports adaptation proteins in female basketball players and male basketball players using proteomics approach on plasma samples withdrawn from athletes during in-season training period but far from a competition. A cohort of 20 professional basketball players, 10 female (BF) and 10 male (BM), and 20 sedentary male (10 CM) and female (10 CF) as control, of comparable age and BMI, were involved in this study. Protein profiles of plasma samples obtained from BM, BF, CM, and CF were analyzed by two-dimensional electrophoresis (2-DE). Differentially expressed proteins were identified by mass spectrometry. The computational 2-DE gel image analysis pointed out 33 differentially expressed protein spots (ANOVA p -value < 0.05) and differences between male and female basketball players are more evident among the players than controls. The expression profile of 54.5% of the total proteins is affected by sports activity. Furthermore, 14 proteins are differentially expressed in basket female players in comparison with their relative controls while seven are differentially expressed in basket male players in comparison with their controls. In conclusion, we identify in female athletes a reduction in proteins related to transcription regulation, most of these modulate chronic inflammation confirming the anti-inflammatory effect of regular training in female muscle metabolism. In male and female athletes, we found a decrease in Transthyretin involved in muscle homeostasis and regeneration and Dermcidin a stress-induced myokine linked to inflammatory and it will be interesting to fully understand the role of its different isoforms in male and female skeletal muscle contraction., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Militello, Pinto, Illiano, Luti, Magherini, Amoresano, Modesti and Modesti.)
- Published
- 2022
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39. Relationships between Sex and Adaptation to Physical Exercise in Young Athletes: A Pilot Study.
- Author
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Pinto G, Militello R, Amoresano A, Modesti PA, Modesti A, and Luti S
- Abstract
The purpose of this study was to compare the redox, hormonal, metabolic, and lipid profiles of female and male basketball players during the seasonal training period, compared to their relative sedentary controls. 20 basketball players (10 female and 10 male) and 20 sedentary controls (10 female and 10 male) were enrolled in the study. Oxidative stress, adiponectin level, and metabolic profile were determined. Male and female athletes showed an increased antioxidant capacity (27% for males; 21% for females) and lactate level (389% for males; 460% for females) and reduced salivary cortisol (25% for males; 51% for females) compared to the sedentary controls. Moreover, a peculiar metabolite (in particular, amino acids and urea), hormonal, and lipidic profile were highlighted in the two groups of athletes. Female and male adaptations to training have several common traits, such as antioxidant potential enhancement, lactate increase, and activation of detoxifying processes, such as the urea cycle and arachidonic pathways as a response to inflammation. Moreover, we found different lipid and amino acid utilization related to sex. Deeper investigation could help coaches in developing training programs based on the athletes' sex in order to reduce the drop-out rate of sporting activity by girls and fight the gender stereotypes in sport that also have repercussions in social fields.
- Published
- 2022
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40. Lscβ and lscγ, two novel levansucrases of Pseudomonas syringae pv. actinidiae biovar 3, the causal agent of bacterial canker of kiwifruit, show different enzymatic properties.
- Author
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Luti S, Campigli S, Ranaldi F, Paoli P, Pazzagli L, and Marchi G
- Subjects
- Kinetics, Actinidia microbiology, Fructans metabolism, Hexosyltransferases chemistry, Plant Diseases microbiology, Pseudomonas syringae enzymology, Pseudomonas syringae isolation & purification
- Abstract
Bacterial canker disease caused by Pseudomonas syringae pv. actinidiae (Psa) biovar 3 involved all global interest since 2008. We have found that in Psa3 genome, similarly to other P. syringae, there are three putative genes, lscα, lscβ and lscγ, coding for levansucrases. These enzymes, breaking the sucrose moiety and releasing glucose can synthetize the fructose polymer levan, a hexopolysaccharide that is well known to be part of the survival strategies of many different bacteria. Considering lscα non-coding because of a premature stop codon, in the present work we cloned and expressed the two putatively functional levansucrases of Psa3, lscβ and lscγ, in E. coli and characterized their biochemical properties such as optimum of pH, temperature and ionic strength. Interestingly, we found completely different behaviour for both sucrose splitting activity and levan synthesis between the two proteins; lscγ polymerizes levan quickly at pH 5.0 while lscβ has great sucrose hydrolysis activity at pH 7.0. Moreover, we demonstrated that at least in vitro conditions, they are differentially expressed suggesting two distinct roles in the physiology of the bacterium., (Copyright © 2021 Elsevier B.V. All rights reserved.)
- Published
- 2021
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41. A mechanistic model may explain the dissimilar biological efficiency of the fungal elicitors cerato-platanin and cerato-populin.
- Author
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Baroni F, Gallo M, Pazzagli L, Luti S, Baccelli I, Spisni A, and Pertinhez TA
- Subjects
- Ceratocystis chemistry, Fungal Proteins chemistry, Models, Molecular, Protein Conformation, Recombinant Proteins chemistry, Recombinant Proteins metabolism, Ceratocystis metabolism, Fungal Proteins metabolism, Oligosaccharides metabolism, Plant Diseases microbiology
- Abstract
Among their various functions, the members of the cerato-platanin family can stimulate plants' defense responses and induce resistance against microbial pathogens. Recent results suggest that conserved loops, also involved in chitin binding, might be a structural motif central for their eliciting activity. Here, we focus on cerato-platanin and its orthologous cerato-populin, searching for a rationale of their diverse efficiency to elicit plants' defense and to interact with oligosaccharides. A 3D model of cerato-populin has been generated by homology modeling using the NMR-derived cerato-platanin structure as template, and it has been validated by fitting with residual dipolar couplings. Loops β1-β2 and β2-β3 have been indicated as important for some CPPs members to express their biological function. When compared to cerato-platanin, in cerato-populin they present two mutations and an insertion that significantly modify their electrostatic surface. NMR relaxation experiments point to a reduced conformational plasticity of cerato-populin loops with respect to the ones of cerato-platanin. The different electrostatic surface of the loops combined with a distinct network of intra-molecular interactions are expected to be factors that, by leading to a diverse spatial organization and dissimilar collective motions, can regulate the eliciting efficacy of the two proteins and their affinity for oligosaccharides., (Copyright © 2021 Elsevier B.V. All rights reserved.)
- Published
- 2021
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42. Redox Homeostasis and Metabolic Profile in Young Female Basketball Players during in-Season Training.
- Author
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Militello R, Luti S, Parri M, Marzocchini R, Soldaini R, Modesti A, and Modesti PA
- Abstract
Background: Most studies on oxidative stress markers and antioxidant levels have been conducted in male athletes, although female participation in sport has increased rapidly in the past few decades. In particular, it could be important to assess oxidative stress markers in relation to the training load because the anaerobic path becomes predominant in high-intensity actions., Methods: Ten female professional basketball players, performing five 2 h-lasting training sessions per week, and 10 sedentary control women were investigated. Capillary blood and saliva samples were collected in the morning before the training session. The antioxidant capacity and the levels of reactive oxygen metabolites on plasma were determined measuring Reactive Oxygen Metabolite and Biological Antioxidant Potential (d-ROMs and the BAP Test). Salivary cortisol was detected by using commercial enzyme-linked immunosorbent assay kit., Results: The antioxidant capacity (BAP value) was significantly higher in elite basketball players (21.2%; p < 0.05). Conversely, cortisol (51%; p < 0.009) and the levels of oxidative species (d-ROM, 21.9%; p < 0.05) showed a significant decrease in elite athletes.
- Published
- 2021
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43. Follicular microenvironment: Oxidative stress and adiponectin correlated with steroids hormones in women undergoing in vitro fertilization.
- Author
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Luti S, Fiaschi T, Magherini F, Modesti PA, Piomboni P, Semplici B, Morgante G, Amoresano A, Illiano A, Pinto G, Modesti A, and Gamberi T
- Subjects
- Adiponectin blood, Adult, Cumulus Cells metabolism, Female, Follicular Fluid metabolism, Humans, Ovary metabolism, Oxidative Stress, Adiponectin metabolism, Cellular Microenvironment, Fertilization in Vitro, Ovarian Follicle cytology, Steroids metabolism
- Abstract
Research has been focused on determining the follicular microenviroment produced by the theca and granulosa cells since the molecular characterisation of this body fluid could lead to the understanding of several fertility problems. Oxidative stress may be one of the factors involved in female infertility since it plays a key role in the modulation of oocyte maturation and finally pregnancy. An increase in oxidative stress is correlated with inflammation and intense research was developed to understand the interaction between inflammation and adiponectin, based on the fact that many adipokines are inflammation related proteins linked to reactive oxygen species production. The aim of this study is to investigate the correlation between total adiponectin levels and oxidative stress amount in the serum and follicular fluid (FF) of women who undergone in vitro fertilization. Moreover we verified the expression of adiponectin in granulosa and cumulus cells. To clarify the predictive value of steroid hormones in human assisted reproduction, twelve steroid hormones in FF and serum, were quantified in a single run liquid chromatography/mass spectrometry, by using a multiple reaction monitoring mode and we related the serum and follicular fluids adiponectin levels with the concentration of the investigated steroid hormones., (© 2020 Wiley Periodicals LLC.)
- Published
- 2021
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44. Inflammation, Peripheral Signals and Redox Homeostasis in Athletes Who Practice Different Sports.
- Author
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Luti S, Modesti A, and Modesti PA
- Abstract
The importance of training in regulating body mass and performance is well known. Physical training induces metabolic changes in the organism, leading to the activation of adaptive mechanisms aimed at establishing a new dynamic equilibrium. However, exercise can have both positive and negative effects on inflammatory and redox statuses. In recent years, attention has focused on the regulation of energy homeostasis and most studies have reported the involvement of peripheral signals in influencing energy and even inflammatory homeostasis due to overtraining syndrome. Among these, leptin, adiponectin, ghrelin, interleukin-6 (IL6), interleukin-1β (IL1β) and tumour necrosis factor a (TNFa) were reported to influence energy and even inflammatory homeostasis. However, most studies were performed on sedentary individuals undergoing an aerobic training program. Therefore, the purpose of this review was to focus on high-performance exercise studies performed in athletes to correlate peripheral mediators and key inflammation markers with physiological and pathological conditions in different sports such as basketball, soccer, swimming and cycling.
- Published
- 2020
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45. Relationship between the metabolic and lipid profile in follicular fluid of women undergoing in vitro fertilization.
- Author
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Luti S, Fiaschi T, Magherini F, Modesti PA, Piomboni P, Governini L, Luddi A, Amoresano A, Illiano A, Pinto G, Modesti A, and Gamberi T
- Subjects
- Adult, Cellular Microenvironment physiology, Female, Follicular Fluid chemistry, Humans, Infertility, Female therapy, Lipids analysis, Metabolic Networks and Pathways physiology, Metabolomics, Oocytes chemistry, Oocytes metabolism, Ovarian Follicle metabolism, Oxidative Stress physiology, Fertilization in Vitro, Follicular Fluid metabolism, Infertility, Female metabolism, Lipid Metabolism, Metabolome
- Abstract
Among the follicular fluid (FF) components promoting the development of the oocyte are included glycoproteins, several fatty acids, and steroid hormones synthesized by the dominant follicle. For this, the analysis of the metabolites present in FF can determine the quality of the oocyte. FF composition is in part determined by local follicular metabolic processes and in part a plasma transudate. Since the causes of impaired fertility may be due to a metabolic imbalance, metabolomics is useful to identify low molecular weight metabolites. Oxidative stress is involved in human infertility and the use of metabolomics can be crucial to identify which other metabolites besides reactive oxygen species are involved in oxidative stress correlated to infertility. To obtain new information on the study of signaling molecules in FF, the knowledge of the lipid content will be important to improve information on the understanding of follicular development. The objective of this study is to identify (a) a metabolic profile and a lipid profile of FF in women undergoing in vitro fertilization and (b) to correlate the previous information obtained regarding adiponectin and oxidative stress with the metabolic and lipid profile obtained in the present study. As result, we found an increase in oxidative stress due to both an increase of androgens and an accumulation of lipids in the follicular environment and we suggest that this might be one of the causes of reduced fertility., (© 2020 Wiley Periodicals LLC.)
- Published
- 2020
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46. Antioxidant and anti-inflammatory properties of sourdoughs containing selected Lactobacilli strains are retained in breads.
- Author
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Luti S, Mazzoli L, Ramazzotti M, Galli V, Venturi M, Marino G, Lehmann M, Guerrini S, Granchi L, Paoli P, and Pazzagli L
- Subjects
- Anti-Inflammatory Agents metabolism, Antioxidants metabolism, Fermentation, Flour analysis, Food Microbiology, Humans, Peptides analysis, Peptides metabolism, Yeasts metabolism, Anti-Inflammatory Agents analysis, Antioxidants analysis, Bread analysis, Bread microbiology, Lactobacillus metabolism
- Abstract
Sourdough fermentation influences several properties of leavened baked goods also because Lactic acid bacteria (LAB) and yeasts produce bioactive peptides with a positive effect on human health. In an early study, three Lactobacilli strains (L. farciminis H3 and A11 and L. sanfranciscensis I4) possessing different proteolytic activities were used to produce sourdoughs containing peptides equipped with anti-inflammatory and/or antioxidant properties. This work was aimed to assess whether these properties could be retained after cooking. The selected LABs were used to produce breads from which low molecular weight (LMW-) peptides were extracted. The results provide solid proofs of keeping both antioxidant and anti-inflammatory activities of peptides from cooked products. Sequences of LMW-peptides either from doughs and breads were determined by mass spectrometry: differences have been noticed in amino acidic composition and in sequences, however, all the strains produce peptides equipped with antioxidant and anti-inflammatory activities., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Published
- 2020
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47. Antioxidant Properties of Sourdoughs Made with Whole Grain Flours of Hull-Less Barley or Conventional and Pigmented Wheat and by Selected Lactobacilli Strains.
- Author
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Galli V, Venturi M, Guerrini S, Blandino M, Luti S, Pazzagli L, and Granchi L
- Abstract
The use of sourdough fermentation and whole grain flours in baked goods manufacturing are known to enhance their functional and nutritional features. In this context, it is necessary to select the most suitable lactic acid bacteria strains and flour combination to achieve this goal. A characterization of 70 lactobacilli strains based on pro-technological and nutritional properties was carried out. The screening allowed the selection of 10 strains that were used to ferment sourdoughs made with two varieties of common wheat, the conventional red-grained cv Aubusson, a blue-grained variety rich in anthocyanins cv Skorpion, and a hull-less barley variety, cv Rondo. From each fermented sourdough, a water soluble extract was obtained and evaluated for its antioxidant activity performed on cultured cells (RAW 264.7 murine macrophage) by assaying Reactive Oxygen Species (ROS) content. Sourdoughs made with pigmented wheat and barley, had an antioxidant activity greater than that recovered in those made with conventional wheat flour, in spite they have been inoculated with the same LAB strains. Results highlighted the interdependence between flour and the inoculated lactic acid bacteria that has to be taken into account for the development of healthy breads exploiting high functional value cereals through biotechnological processes.
- Published
- 2020
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48. S-Homocysteinylation effects on transthyretin: worsening of cardiomyopathy onset.
- Author
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Leri M, Rebuzzini P, Caselli A, Luti S, Natalello A, Giorgetti S, Marchese L, Garagna S, Stefani M, Paoli P, and Bucciantini M
- Subjects
- Amyloid Neuropathies, Familial pathology, Cardiomyopathies pathology, Cardiovascular Diseases genetics, Cardiovascular Diseases pathology, Diabetes Mellitus, Type 2 genetics, Diabetes Mellitus, Type 2 pathology, Homocysteine chemistry, Humans, Methionine chemistry, Mutation genetics, Myocytes, Cardiac, Prealbumin genetics, Prealbumin ultrastructure, Protein Conformation, Protein Stability, Stroke genetics, Stroke pathology, Structure-Activity Relationship, Amyloid Neuropathies, Familial genetics, Cardiomyopathies genetics, Homocysteine genetics, Prealbumin chemistry
- Abstract
Background: L-Homocysteine (Hcy) is a non-proteinogenic α-amino acid synthesized from dietary methionine. In healthy humans, high Hcy levels are a risk factor for cardiovascular diseases, stroke and type 2 diabetes. A recent study reports that Hcy reacts with Cys
10 of transthyretin (TTR), generating a stable covalent adduct. However, to date the effect of S-homocysteinylation on TTR conformational stability remains unknown., Methods: The effect of Hcy on the conformational properties of wt- and L55P-TTR were analysed using a set of biophysical techniques. The cytotoxicity of S-homocysteinylated L55P-TTR was also evaluated in the HL-1 cardiomyocyte cell line, while the effects of the assemblies on kinematic and dynamics properties of cardiac muscle cells were analysed in cardiomyocyte syncytia., Results: We found that Hcy stabilizes tetrameric wt-TTR, while it destabilizes the tetrameric structure of the L55P mutant, promoting the accumulation of self-assembly-prone monomeric species., Conclusions: Our study demonstrated that S-homocysteinylation of the L55P-TTR mutant impairs protein stability, favouring the appearance of toxic monomers. Interestingly, S-homocysteinylation affected only mutant, not wt-TTR. Moreover, we also show that assemblies of S-homocysteinylated L55P-TTR impair cardiomyocytes functional parameters., General Significance: Our study offers new insights on the negative impact of S-homocysteinylation on L55P-TTR stability, whose aggregation is considered the causative agent of a form of early-onset familial amyloid polyneuropathy and cardiomyopathy. Our results suggest that high homocysteine levels are a further risk factor for TTR cardiomyopathy in patients harbouring the L55P-TTR mutation., (Copyright © 2019 Elsevier B.V. All rights reserved.)- Published
- 2020
- Full Text
- View/download PDF
49. Isolation and characterization of soluble human full-length TDP-43 associated with neurodegeneration.
- Author
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Vivoli Vega M, Nigro A, Luti S, Capitini C, Fani G, Gonnelli L, Boscaro F, and Chiti F
- Subjects
- Amino Acid Sequence, Amyotrophic Lateral Sclerosis genetics, Amyotrophic Lateral Sclerosis metabolism, Chromatography, Gel, Circular Dichroism, Cloning, Molecular, DNA-Binding Proteins chemistry, DNA-Binding Proteins genetics, Dynamic Light Scattering, Frontotemporal Lobar Degeneration genetics, Frontotemporal Lobar Degeneration metabolism, Humans, Mass Spectrometry, Neurodegenerative Diseases genetics, Protein Folding, Protein Stability, Protein Structure, Quaternary, Recombinant Proteins chemistry, Recombinant Proteins genetics, Recombinant Proteins isolation & purification, Solubility, DNA-Binding Proteins isolation & purification, Neurodegenerative Diseases metabolism
- Abstract
The involvement of transactivation response (TAR) DNA-binding protein 43 (TDP-43) in neurodegenerative diseases was revealed in 2006, when it was first reported to be the main component of the intracellular inclusions in patients with amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration. After 12 yr it is not yet possible to purify to a reasonable yield and in a reproducible manner a stable full-length protein, which has limited so far the characterization of its structure, function, molecular interactors, and pathobiology. Using a novel protocol we have achieved the purification of the full-length TDP-43, with both a short pectate lyase B tag and a glutathione S -transferase tag, which consisted in its expression in bacteria, solubilization from inclusion bodies, purification under denaturing conditions, refolding, and a final size exclusion chromatography (SEC) step. Differential scanning fluorimetry was used to find the best buffers and combination of additives to increase both its solubility and its stability. The protein is pure, as determined with electrophoresis, Western blotting, and mass spectrometry; properly refolded, as revealed by circular dichroism and fluorescence spectroscopies; functional, because it binds to DNA and protein partners; and stable to degradation and aggregation in a physiologic solution. Analyses with dynamic light scattering and SEC revealed that the protein is a dimer.-Vivoli Vega, M., Nigro, A., Luti, S., Capitini, C., Fani, G., Gonnelli, L., Boscaro, F., Chiti, F. Isolation and characterization of soluble human full-length TDP-43 associated with neurodegeneration.
- Published
- 2019
- Full Text
- View/download PDF
50. Alternative responses to fungal attack on a metalliferous soil: Phytohormone levels and structural changes in Silene paradoxa L. growing under copper stress.
- Author
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Papini A, Luti S, Colzi I, Mazzoli L, Giorni E, Pazzagli L, and Gonnelli C
- Subjects
- Adaptation, Physiological, Plant Diseases microbiology, Silene growth & development, Silene metabolism, Copper adverse effects, Fungi physiology, Plant Growth Regulators metabolism, Silene drug effects, Silene microbiology, Soil Pollutants adverse effects
- Abstract
In this work, a non-metallicolous and a metallicolous population of S. paradoxa were exposed to copper excess and fungal elicitation, and investigated for phytohormone production and cytological alterations. Under the stress applied separately and in combination, S. paradoxa plants varied phytohormone concentration in a population-specific way, suggesting a different signalling in response to biotic and abiotic stimuli according to the environment of origin. Generally, the stress responses consisted in increased levels of salicylic acid, auxin, and gibberellin in the non-metallicolous population, and of jasmonic and abscisic acid in the metallicolous one. Interestingly, the metallicolous population increased the level of such phytohormones following exposure to the fungal elicitor only in the presence of copper. This alternative hormonal signalling could derive from the incompatibility between the ordinary ROS-mediated response to pathogens and the acquired mechanisms that prevent oxidative stress in the population from the metal-rich soil. Furthermore, stress-induced autophagic phenomena were more evident in the non-metallicolous plants than in the metallicolous ones, suggesting that the adaptation to the metalliferous environment has also affected autophagy intensity and signalling in response to copper excess and fungal elicitation., (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Published
- 2019
- Full Text
- View/download PDF
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