1. Cdk5 is required for the neuroprotective effect of transforming growth factor-β1 against cerebral ischemia-reperfusion.
- Author
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Zhao, Wenhui, Yan, Jing, Gao, Liyun, Zhao, Jinfeng, Zhao, Conglin, Gao, Chao, Luo, Xiaoqiu, and Zhu, Xinshu
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NEUROPROTECTIVE agents , *CEREBRAL ischemia , *TRANSFORMING growth factors , *NEUROTROPHINS , *CENTRAL nervous system , *REPERFUSION injury - Abstract
Transforming Growth Factor β1 (TGF-β1), a well-known neuroprotective and neurotrophic factor in the central nervous system, is also involved in the repair process responses after ischemia-reperfusion injury. Herein, we found that TGF-β1 enhanced Cdk5 expression while decreased Tunel-positive cells compared with the ischemia group, and roscovitine(Cdk5 inhibitor) treatment could blunt these effects. In vitro study, TGF-β1 facilitated Cdk5/p35 complex, the proliferation, neurite growth and differentiation of PC12 cells, effects of which could be blunted by roscovitine and Cdk5 silencing. Moreover, ERK1/2 inhibitor SCH772984 abrogated the effects of TGF- β1 on Cdk5 and Bax levels. Taken together, we conclude that Cdk5 contributes to the neuroprotective function of TGF- β1 via ERK1/2 signaling. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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