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Abstract The Qinghai-Tibetan Plateau (QTP) is vital for stabilizing climate in East and South Asia and its glaciers and snow meltwater nourish key continental rivers. The region’s forests are not only ecologically essential but also fragile. The practices of local herdsmen, shaped by long-standing cultural traditions, significantly affect forest dynamics. This study examines the vital role that Tibetan Buddhist monasteries play in forest conservation on the QTP. We utilize data sources including extensive field surveys and geospatial data to assess the impact of monastery and village locations on forest conditions. Spatial analysis techniques, such as Moran’s I and Ripley’s K function, reveal significant clustering of monasteries and villages, which are closely linked with forest conditions. Our findings indicate that forests near Buddhist monasteries have higher aboveground biomass and better quality compared to other areas, suggesting that their presence supports better forest conservation practices. This positive influence is attributed to the cultural significance of these sites and the environmental consciousness promoted by Buddhist cultural teachings. Additionally, this study employs analytical methods including Random Forest and Partial Least Squares Structural Equation Modeling (PLS-SEM) to explore the drivers of forest quality. Initial aboveground biomass, climatic factors, and monastery density emerge as key influences on forest quality within monastery buffer zones, indicating that cultural factors are crucial in shaping forest landscapes.

Abstract With the far-reaching consequences of worldwide climate variationon ecosystems and human societies, understanding and predicting changes in cultural ecosystem services (CES) is essential for sustainable development policy and resource planning. Past studies have focused on changes and impacts on natural ecosystems, while relatively few studies have been conducted on predictions of CES. This study combines POI datasets with future climate variables under different scenarios into the Maxent model for forecasting the spatial and temporal distribution of CES, which provides strong support for future decision-making. The results indicate that: (1) Under the SSP126 and SSP585 scenarios, the CES values in the northern, western, central, and northeastern parts of the study area are relatively high, while those in the southwestern, southern, and southeastern parts are relatively low. (2) Under the SSP126 scenario, the total CES shows an increasing trend from 2021 to 2040, but slightly declines from 2061 to 2080. In contrast, under the SSP585 scenario, the total CES significantly decreases from 2021 to 2040, especially in the provinces of Guizhou, Hunan, Zhejiang, and Anhui. (3) Temperature has a significant impact on CES predictions, with the annual mean temperature (Bio1) positively correlated with total CES, contributing between 0.75 and 0.78 to the distribution of CES across different years and scenarios. Additionally, the maximum temperature of the hottest month (Bio5) and the mean temperature of the wettest quarter (Bio8) also significantly influence CES under different scenarios and years. These findings reveal the regional characteristics and variations in CES distribution under different climate scenarios, providing crucial scientific evidence for future policy-making, resource management, and climate adaptation strategies. They also offer important insights into the impact of global climate change on ecosystems and human society, serving as a valuable reference for future national decision-making.

Abstract Over 700 syndromes associated with hearing loss (HL) have been identified. Labyrinthine aplasia, microtia, and microdontia (LAMM syndrome, OMIM: 610706) is a rare HL syndrome characterized by congenital sensorineural HL, labyrinthine aplasia, type I microtia and microdontia, which is caused by biallelic variants in the FGF3 gene. Using Whole-exome sequencing (WES), we identified a novel missense FGF3 variant (c.137G > C, p. Arg46Pro (NM_005247.4) in three unrelated Uyghur ethnic families. This variant is classified as a variant of uncertain significance according to ACMG guidelines, with the applied criteria of PM3, PM2_Supporting, PP3 and PP4. Patients from the three families revealed variable clinical features. We found a novel phenotype, sparse hair, in one of the proband. Our findings expanded the variant and phenotype spectrum of LAMM syndrome and provided new insights to the diagnose and pathogenesis investigation of the disease.

Studying the coupled relationship between social-ecological systems (SES) provides a more comprehensive understanding of the impact of human activity on ecosystems, thereby facilitating the formulation of more rational environmental protection policies. A deeper understanding of this relationship can promote the sustainable utilization and management of the environment. With its abundant natural resources, Ganzhou City represents the most typical ecological location in the southern hilly region of China. In this study, we constructed a sustainable framework for composite factors to analyze their effects on the ecosystem services supply (ESS). This framework emphasizes the positive roles assessment indicators such as water, forests, lakes, grasslands, and governance have on ESS, which are directly linked to the objectives of Sustainable Development Goal (SDG) 15, which include forest conservation, biodiversity maintenance, and water resource management. The results indicate that: (1) From 2005 to 2020, the overall ESS index in the central and western regions of Ganzhou showed a trend of being lower in the north and higher in the south, with areas outside the central and western regions showing a yearly increase. (2) Water, forest, lake, grassland, and governance indicators have a positive impact on the total ESS, while indicators such as mountains, sand, society, and actors have a negative impact. (3) From 2005 to 2020, the coupling coordination relationship in the central-northern and southern regions of Ganzhou gradually strengthened, with most areas shifting from a dysfunctional state to a low coupling state. By 2030, the total ESS index in counties such as Ningdu, Yudu, Huichang, Chongyi, and Xunwu is expected to decline. Some northeastern and southern regions are projected to show an upward trend by 2035. This study will significantly contribute to the realization of SDG 15, as it integrates closely related composite factors into a sustainable SES framework, by further delving into the factors influencing ESS and providing practical guidance for achieving SDGs.

Abstract Magnetic frustration is a route for novel ground states, including spin liquids and spin ices. Such frustration can be introduced through either lattice geometry or incompatible exchange interactions. Here, we find that epitaxial strain is an effective tool for tuning antiferromagnetic exchange interactions in a square-lattice system. By studying the magnon excitations in La2NiO4 films using resonant inelastic x-ray scattering, we show that the magnon displays substantial dispersion along the antiferromagnetic zone boundary, at energies that depend on the lattice of the film’s substrate. Using first principles simulations and an effective spin model, we demonstrate that the antiferromagnetic next-nearest neighbour coupling is a consequence of the two-orbital nature of La2NiO4. Altogether, we illustrate that compressive epitaxial strain enhances this coupling and, as a result, increases the level of incompatibility between exchange interactions within a model square-lattice system.

Spatial characteristics and geographical scales are crucial factors influencing the functioning of ecosystem services (ESs), especially the spatial flows that transcend territorial boundaries. A comprehensive understanding of the spatiotemporal evolution of these spatial spillover effects enriches our knowledge on the distribution and temporal dynamics of ecosystem service functions. By examining the spatiotemporal evolution of ecosystem service flow effects of the Yangtze River Economic Belt (YREB), the study offers scientific evidence underpinning the sustainable development of ESs among different regions. To this end, this study undertook the computation of ecosystem service values (ESV) and utilized an improved gravitational model to establish the ESV spatial correlation network (SCN) in the YREB. Subsequently, social network analysis (SNA) was employed to examine the spatiotemporal evolution of the ESV-SCN. Finally, an analytical framework was constructed to scrutinize the factors that influence the ESV-SCN under the influences of both natural and socio-economic factors. The results suggest: 1) Between the years 1990 and 2020, ESV within the YREB exhibited substantial spatial correlations and overflow effects, with a notable 3.49% increase in network density; 2) The hierarchical structure of the ESV-SCN witnessed a 44.51% decline, while network efficiency witnessed an 8.47% increase. Moreover, network accessibility strengthened, and the discernible presence of multiple overlapping effects contributed to heightened stability; 3) Both within and between various sectors of the ESV-SCN, substantial linkages and cascading effects were noted. The spatiotemporal evolution of the SCN exhibited distinct hierarchical and clustering patterns, with the complexity of the network structure steadily augmenting; 4) Natural factors such as temperature, precipitation, the proportion of forest land, alongside socio-economic factors like the level of economic development and innovation, all played indispensable roles in shaping and nurturing the ESV spatial correlation networks.

Narcolepsy type 1 (NT1) is caused by a loss of hypocretin/orexin transmission. Risk factors include pandemic 2009 H1N1 influenza A infection and immunization with Pandemrix®. Here, we dissect disease mechanisms and interactions with environmental triggers in a multi-ethnic sample of 6,073 cases and 84,856 controls. We fine-mapped GWAS signals within HLA (DQ0602, DQB1*03:01 and DPB1*04:02) and discovered seven novel associations (CD207, NAB1, IKZF4-ERBB3, CTSC, DENND1B, SIRPG, PRF1). Significant signals at TRA and DQB1*06:02 loci were found in 245 vaccination-related cases, who also shared polygenic risk. T cell receptor associations in NT1 modulated TRAJ*24, TRAJ*28 and TRBV*4-2 chain-usage. Partitioned heritability and immune cell enrichment analyses found genetic signals to be driven by dendritic and helper T cells. Lastly comorbidity analysis using data from FinnGen, suggests shared effects between NT1 and other autoimmune diseases. NT1 genetic variants shape autoimmunity and response to environmental triggers, including influenza A infection and immunization with Pandemrix®.

Abstract Background Treacher Collins syndrome (TCS; OMIM 154500) is a craniofacial developmental disorder. Methods To investigate the genetic features of a four-generation Chinese family with TCS, clinical examinations, hearing tests, computed tomography, whole-exome sequencing (WES), Sanger sequencing, reverse transcription (RT)-PCR, and the Minigene assay were performed. Results The probands, an 11-year-old male and his cousin exhibited typical clinical manifestations of TCS including conductive hearing loss, downward slanting palpebral fissures, and mandibular hypoplasia. Computed tomography revealed bilateral fusion of the anterior and posterior stapedial crura and malformation of the long crura of the incus. WES of both patients revealed a novel heterozygous intronic variant, i.e., c.4342 + 5_4342 + 8delGTGA (NM_001371623.1) in TCOF1. Minigene expression analysis revealed that the c.4342 + 5_4342 + 8delGTGA variant in TCOF1 caused a partial deletion of exon 24 (c.4115_4342del: p.Gly1373_Arg1448del), which was predicted to yield a truncated protein. The deletion was further confirmed via RT-PCR and sequencing of DNA from proband blood cells. A heterozygous variant in the POLR1C gene (NM_203290; exon6; c.525delG) was found almost co-segregated with the TCOF1 pathogenic variant. Conclusions In conclusion, we identified a heterozygous TCOF1 splicing variant c.4342 + 5_4342 + 8delGTGA (splicing) in a Chinese TSC family with ossicular chain malformations and facial anomalies. Our findings broadened the spectrum of TCS variants and will facilitate diagnostics and prognostic predictions.

The balance between the supply and demand of ecosystem services (ESSD) is important for sustainable regional development. The hilly regions of south China have rich natural resources and are rapidly being urbanized, which significantly impacts sustainable development in the region. Therefore, how to analyze ESSD from the perspective of social ecology is imperative. Ganzhou region, which is a typical hilly region in southern China, as an example, this paper creatively integrated ESSD into the social-ecological system (SES) framework, analyzed the spatial and temporal changes of ESSD in 2005, 2010, 2015, and 2020, and constructed a system of indicators from the SES, and analyzed the driving factors of ESSD, so as to provide a reference for balancing ESSD. Based on the classical SES framework proposed by Ostrom, this study first analyses the social-ecological system profile of the Ganzhou region at the macro-scale and constructs an SES indicator system. The innovation of combining the SES framework and ecosystem services lies in integrating the social and ecosystem perspectives and merging these two concepts to understand better and manage the relationship between humans and the natural environment. It provides a comprehensive analytical approach and decision support tool for achieving sustainable development. Our results demonstrate that: (1) From 2005 to 2020, ecosystem services in the study area were highly valued, lower areas had homogeneous distribution characteristics, total demand displayed spatial differentiation, there were high-value areas in the midwest, and low-value areas were concentrated in the region’s boundary. (2) There are obvious supply and demand deficits in the southeast and east of the study area, while supply–demand is equal in the north and supply exceeds demand in other regions. From 2005 to 2020, the overall coupling coordination degree of ESSD in the study area was lower than 0.5, indicating a mild to moderate imbalance. (3) The supply of ecosystem services (ESS) was primarily driven by carbon storage, habitat quality, and water yield, while the demand was primarily driven by forest and grassland areas. Factors related to the ecosystem, resource unit, resource system, social system, governance system, and actor all have experienced two-factor interactions, which can explain up to 99% of ESSD. Based on the SES framework, this paper studies the relationship between ESSD in typical areas of the southern hills to provide a reference for research on ESSD in relevant areas and provide a scientific basis for the Chinese government to evaluate ecological protections and improve compensation policies, which is important for ensuring China's ecological security.

Abstract Persistent room temperature phosphorescent materials with unique mechanical properties and robust optical properties have great potential in flexible electronics and photonics. However, developing such materials remains a formidable challenge. Here, we present highly stretchable, lightweight, and multicolored persistent luminescence elastomers, produced by incorporating ionic room temperature phosphorescent polymers and polyvinyl alcohol into a polydimethylsiloxane matrix. These prepared elastomers exhibit high optical transparency in daylight and emit bright persistent luminescence after the removal of 365 nm excitation. The homogeneous distribution of polymers within the matrix has been confirmed by confocal fluorescence microscopy, scanning electron microscopy, and atomic force microscopy. Mechanical property investigations revealed that the prepared persistent luminescence elastomers possess satisfactory stretchability. Impressively, these elastomers maintain robust optical properties even under extensive and repeated mechanical deformations, a characteristic previously unprecedented. These fantastic features make these persistent luminescence elastomers ideal candidates for potential applications in wearable devices, flexible displays, and anti-counterfeiting.

Over the past two decades, due to the combined effects of natural and human factors, the ecological environment and resources of the Qinghai–Tibet Plateau (QTP) have faced serious threats, profoundly impacting its ecosystem and the lives of its residents. Therefore, the establishment of the ecological security pattern (ESP) is crucial to cope with climate change, maintain ecosystem function, and sustainable development. Based on the Pressure–State–Response (PSR) model, this study constructed an evaluation index system for the ecological security (ES) of the QTP, evaluated the ES of the QTP during 2000–2020, and predicted the ES of the QTP during 2025–2035 based on the deep learning model. Combined with the residents’ perception of ES, the ES of the QTP was evaluated comprehensively. The results showed that: (1) From 2000 to 2020, the ES value of the QTP continued to rise, the number of dangerous and sensitive counties decreased, and the number of other counties increased. The overall spatial distribution features higher values in the southeast and lower values in the northwest and central regions. (2) From 2000 to 2020, both hot spots and cold spots on the QTP decreased, with the hot spots mainly concentrated in the southeast of the QTP, represented by Yunnan Province, and the cold spots shifting from west to east, mainly concentrated in the central QTP, represented by Qinghai Province. (3) The Long Short-Term Memory (LSTM) model demonstrates high prediction accuracy. Based on the prediction of LSTM, the ES value of the QTP will continue to rise from 2025 to 2035, and the number of safe counties will reach the highest level in history. The spatial distribution is still higher in the southeast and lower in the northwest and central regions. (4) By analyzing residents’ perception of 25 potential factors that may affect the ES of the QTP, the results show that residents generally believe that these factors have an important impact on ES, and their evaluation is between “important” and “very important”. In addition, there is a significant correlation between these factors and the predicted values of ES. The results of the study will help to improve our understanding of the overall ecological environment of the QTP, provide accurate positioning and reasonable help for the government to formulate relevant protection strategies, and lay a methodological and practical foundation for the sustainable development of the QTP.

Sustainability has become an indispensable core consideration when nations formulate their development policies. This study delves into the dynamic correlations between ecosystem services (ESs) and localized Sustainable Development Goals (SDGs) in a typical hilly region in southern China. Various ESs were computed using the InVEST model, while spatial econometric models were employed to assess ES responses to SDG targets at the county, sectoral, and overall spatial levels. The findings revealed the following. (1) From 2005 to 2020, there were differences in the development rates of various SDG targets in Ganzhou. Except for SDG 15, which declined, the development of the other targets showed an overall increasing trend. The development of the various SDG targets was relatively balanced, but SDG 9 and SDG 7 had the highest growth rates, ranging from 9.4% to 10.7% and 9.4% to 10.3%, respectively. The comprehensive SDG assessment index exhibited significant spatiotemporal variation, with a general trend of higher values in the north and lower values in the south. (2) The local ES showed a delayed response to SDG 1 and SDG 2, but there was a significant positive response to SDG 3, SDG 4, SDG 6, SDG 8, and SDG 9. However, responses to SDG 7, SDG 11, SDG 13, and SDG 15 showed regional differences. This study not only provides valuable insights for sustainable development in Ganzhou and other regions of China but also offers Chinese perspectives and experiences that may inform global efforts towards SDG implementation. This study fills the gap in existing research by constructing a localized SDG indicator system and quantifying each SDG indicator, further exploring the response of the ES to each SDG in the region. Looking ahead, we anticipate further research to deepen the understanding of the relationship between ESs and SDG targets on a broader geographical scale and over longer timeframes, aiming to provide a more robust scientific foundation for building a harmonious coexistence between humans and nature in the future.

The scientific quantification of the ecological effects of carbon emissions, the reduction of ecological risk (ER), and the evaluation of the interaction effect between carbon emissions and ER are the pivotal measures for ensuring the longevity and continuity of sustainability. However, a dearth of comprehensive and macro-level evaluations exist pertaining to the ER and carbon emissions within the entirety of the Yangtze River Economic Belt (YREB). We have constructed four distinct simulated scenarios within the YREB, which include natural development (ND), cultivated land protection (CLP), ecological conservation (EC), and low carbon (LC) scenarios. Based on the consideration of future uncertainty, we predicted LUCEs and ERI under different scenarios, and analyzed the spatial interaction effects of LUCEs and ERI from the dual perspectives of the spatial spillover effect and spatial coupling effect. The results showed that under the four outlined scenarios, encompassing diverse parameters, conversion possibilities, and areas subject to restrictions, the land utilization patterns of the YREB in 2030 have unveiled pronounced disparities. The LUCEs in the YREB showed significant spatial heterogeneity under the four scenarios; the maximum value was 6.65 under the CLP scenario and the minimum value was 4.65 under the LC scenario. The ER has the highest value under the ND scenario and the lowest value under LC scenario. Construction land is the largest contributor to increased LUCEs, and forest land is the form of terrestrial utilization that reduces the impact of LUCEs. In different scenarios, LUCEs have a significant negative spillover effect on ER, while the bidirectional spatial coupling effect between LUCEs and ERI presents significant differences. Under the LC scenario, land with a strong carbon sequestration capacity increased significantly, the fragmentation of water bodies was alleviated, and the CCD was the highest. This study offers scientific counsel for the sustainable development of various regions within the YREB, thereby fostering the achievement of a harmonious coexistence between the ecological milieu and economic development.

Achieving “carbon neutrality” is an inevitable requirement for tackling global warming. As one of the national ecological barriers, the southern hilly and mountainous region (SHMR) shoulder the important mission of taking the lead in achieving “carbon peak” and “carbon neutrality”. Thus, it has important scientific significance to explore and analyze how to coordinate ecological development under the background of “double carbon action”, and it is a key step to ensure that the region achieves synergistic development of promoting economic development and improving ecosystem health. Therefore, in this study, we aimed to address these gaps by adopting a refined grid scale of 10 km × 10 km to explore the spatial-temporal distribution characteristics of carbon emissions and ecosystem health. Additionally, we established a coupling coordination model of carbon emissions intensity (CEI) and ecosystem health index (EHI) to assess the impact of natural and socio-economic factors on the coupling coordination degree (CCD) in different regions. Our findings are as follows: 1) In the SHMR region, the EHI exhibited a progressive development trend, with spatially increasing values from the south to the north. 2) The spatial discrepancy in CEI has been on the rise, which assumed an increase of 4.69 times, and with an increasingly pronounced pattern of spatial imbalance. Carbon emissions tend to concentrate more in the eastern and northern areas, while they are comparatively lower in the western and southern regions. 3) The R2 of geographical weighted regression model (GWR) is all above 0.8, and the CCD between CEI and EHI demonstrated a positive developmental state. However, most regions still displayed an imbalanced development, albeit with a slight increase in areas exhibiting a more balanced development state. 4) The driving forces of natural and socio-economic factors had a dual-factor and non-linear enhancement effect on the CCD. The influence of natural factors on CCD has gradually diminished, whereas the influence of socio-economic factors has progressively strengthened.

Abstract Studying the impact of large-scale ecological projects, such as the Grain-for-Green Program (GGP), on ecosystem services (ES) is currently a frontier and hot topic of ecological research. The GGP can directly change land use and land cover, thus affecting ES. By comparing the changes of ecosystem service value (ESV) and ecosystem service scarcity value (ESSV) in Qinghai before and after the implementation of the GGP, this paper clarified the impact of the GGP on Qinghai from the angles of ecology and economics. This paper quantified and evaluated the land use dynamics, ESV, and ESSV in Qinghai from 1995 to 2020. The results showed that in the past 25 years, the total annual Normalized Difference Vegetation Index (NDVI) of Qinghai showed a trend of sustained growth. From 1995 to 2020, the ESV increased by 6.80%. After considering supply and demand, the ESSV showed a continuous upward trend, increasing by 719.38%. After implementation of the GGP, the increase of NDVI inhibited the increase of the ESSV. These findings from evaluation of the effect of the GGP implementation provide a theoretical basis for future policy implementation and, in particular, a reference for the evaluation of the ESV and the ESSV in Qinghai.

Summary: Functional cochlear hair cells (HCs) innervated by spiral ganglion neurons (SGNs) are essential for hearing, whereas robust models that recapitulate the peripheral auditory circuity are still lacking. Here, we developed cochlear organoids with functional peripheral auditory circuity in a staging three-dimensional (3D) co-culture system by initially reprogramming cochlear progenitor cells (CPCs) with increased proliferative potency that could be long-term expanded, then stepwise inducing the differentiation of cochlear HCs, as well as the outgrowth of neurites from SGNs. The function of HCs and synapses within organoids was confirmed by a series of morphological and electrophysiological evaluations. Single-cell mRNA sequencing revealed the differentiation trajectories of CPCs toward the major cochlear cell types and the dynamic gene expression during organoid HC development, which resembled the pattern of native HCs. We established the cochlear organoids with functional synapses for the first time, which provides a platform for deciphering the mechanisms of sensorineural hearing loss.

Regional ecological risk assessment (ERA) is an important means towards sustainable regional development. Yet, existing ERA approaches often fall short of characterizing ecological processes as dynamic processes involving uncertainty. Using Inner Mongolia, a high ecological risk area in China, this study employed a framework for regional ERA that considers ecosystem service value (ESV) and four scenarios of future land use patterns to understand its regional ecological risk. The spatially explicit risk assessment method was implemented by a model combining multi-criteria evaluation (MCE), cellular automata (CA), and Markov chain to predict future land use/cover of 2030, an adjustment-based equivalence factor method to assess the ESV in each scenario, and Sharpe Index to assess regional ecological risk. The results show the following. First, the overall ESV increases from 2020 to 2030 under all but the socio-economic development (SED) scenarios considered in this study. Second, the overall regional ecological risk of Inner Mongolia is high in the central region and low in the east, north, and west regions. The low ecological risk areas account for the largest areal proportion in the ecosystem services protection (ESP) and natural development (ND) scenarios, while the medium ecological risk areas are the largest under SED. However, some low ecological risk areas are converted into medium ecological risk in ecological and socioeconomic balance scenario (ESB) scenario. Third, NDVI is the driving factor with the strongest explanatory power for the regional ecological risk in Inner Mongolia, followed by mean annual precipitation and temperature. The present study applied a framework for regional ERA that explicitly considers spatial heterogeneity and uncertainty, which can be useful for guiding decision-making for ecological risk control and the planning of the land use of ecosystems.

As one of the world’s critical ecological regions – often called the “Third Pole” – the Qinghai-Tibet Plateau (QTP) has outstanding ecosystem service values (ESV). However, its variable climate and fragile environmental conditions make it vulnerable to disturbance by human activities. Based on land use data from 2000, 2010, and 2020, we developed three simulated scenarios of land use distribution on the QTP: business as usual (BAU), farmland protection (FP), and ecological protection (EP). Finally, we employed the Sharpe Index to evaluate ecological risk across the QTP. The results showed that from 2000 to 2020, land use change was mainly in the form of conversion of grassland to unused land, totaling an area of 63,717 km2. The ecosystem service value showed an increasing trend, from US$752.6 billion to US$783.0 from 2010 to 2020. The simulation results from the three scenarios revealed significant differences. Farmland area shows a declining trend in both the EP and BAU scenarios, while exhibiting an increasing trend in the FP scenario. Forest area on the plateau experiences a decreasing trend in the EP and BAU scenarios, but shows an increase in the FP scenario, predominantly in the southeastern region, including Sichuan and Yunnan provinces. The ecological risk assessment results indicate that the eastern region has higher ecological risk compared to the western region, highlighting the western region as a priority area for risk control. The expansion of built-up land and unused land has had significant impacts on regional ecological risk. Integrating risk assessment outcomes with land use planning to identify focal areas can provide guidance for the sustainable development of this globally important ecoregion.

With the rapid progress in urbanization and economic development, the impact of land use change (LUC) on ecosystem services is becoming increasingly significant. However, the accuracy of ecological risk assessment faces challenges due to the presence of uncertainty factors. Using the PLUS model, this study aims to simulate and predict land use changes (LUCs), focusing on the southern hilly regions in southeastern China as a case study, conducting an in-depth assessment of ecological risk uncertainty. Firstly, a spatiotemporal simulation of LUCs in the southern hilly region from 1990 to 2030 was conducted under multiple scenarios. Subsequently, differences in the spatial and temporal distribution of ecosystem service value (ESV) across different years and forecast scenarios in the southern hilly region were revealed, followed by a detailed analysis of the impact of LUCs on ESV. Finally, by calculating the Ecological Risk Index (ERI), the study systematically analyzed the evolution trend of ecological risk in the southern hilly region of China from 1990 to 2030. The main research findings are as follows: (1) the conversion proportions of different land use types vary significantly under different scenarios. Compared to 2020, under the 2030 National Development Scenarios (NDSs), there has been a slight decrease of around 3% in the total conversion area of farmland, forest, and grassland. However, under the Ecological Protection Scenario (EPS) and Urban Development Scenario (UDS) scenarios, there has been an increase in the area of forest and grassland, with a rise of approximately 1.5% in converted built-up land. (2) Western cities (e.g., Yueyang and Yiyang), central cities (e.g., Jiujiang), and northeastern cities (e.g., Suzhou) of China exhibit a relatively high ESV distribution, while ESV significantly decreased overall from 2010 to 2020. However, under the EPS and UDS, ESV shows a significant increasing trend, suggesting that these two scenarios may play a crucial role in ecosystem restoration. (3) The conversion of forest and water bodies to farmland has the most significant inhibitory effect on ESV, especially during the period from 1990 to 2000, providing substantial data support for relevant policy formulation. (4) From 1990 to 2030, ecological risk gradually increased in western, central, and southwestern cities of the southern hilly region, with the highest ecological risk values under the EPS scenario in northern cities (e.g., Chizhou and Tongling). Under the UDS scenario, there has been a significant decrease in ecological risk, providing valuable insights for future ecological conservation and sustainable development. However, a limitation lies in the need for further enhancement of the scenario’s simulation authenticity. This study offers a new perspective for understanding the impact of LUCs on ecosystem services and the uncertainty of ecological risks, providing crucial reference points for land resource management and the formulation of ecological conservation policies.

BackgroundGlucagon-like peptide-1 (GLP-1) has been reported to have beneficial impacts on improving human's metabolism and ameliorating insulin resistance. While insulin is another important and conventional drug in diabetes treatment, but it has an adverse effect on weight gain.PurposeTo make sure whether GLP-1 and insulin play different roles in human adipose-derived stem cells (hADSCs).MethodsWe examined the in vitro roles and molecular mechanisms of liraglutide, a GLP-1 analogue, and human insulin on hADSCs isolated from subcutaneous adipose tissue. Different concentrations (0, 0.1, 1, 10, 100nM) of liraglutide and insulin were added to proliferation and differentiation medium of hADSCs, respectively.ResultsLiraglutide inhibits while insulin promotes the proliferation and differentiation at the concentration of 100nM. Moreover, the levels of GSK-3 increase during differentiation and liraglutide could down-regulate it when compared with insulin. We also find that the activation of phosphorylated GSK-3α and GSK-3β is involved in the differentiation roles. And classical and non-classical Wnt pathways all play roles in the differentiation, which are characterized with the up/down-regulation of the expression of adipogenesis genes such as PPAR-γ and CEBP-α.ConclusionLiraglutide and insulin have contrary effects on the proliferation and adipogenesis via Wnt pathway in primary cultured ADSCs. Those effects could partly explain the different roles of GLP-1 and insulin on weight gain and insulin resistance.

Abstract Mammalian sensory hair cells (HCs) have limited capacity for regeneration, which leads to permanent hearing loss after HC death. Here, we used in vitro RNA-sequencing to show that the Hippo signaling pathway is involved in HC damage and self-repair processes. Turning off Hippo signaling through Mst1/2 inhibition or Yap overexpression induces YAP nuclear accumulation, especially in supporting cells, which induces supernumerary HC production and HC regeneration after injury. Mechanistically, these effects of Hippo signaling work synergistically with the Notch pathway. Importantly, the supernumerary HCs not only express HC markers, but also have cilia structures that are able to form neural connections to auditory regions in vivo. Taken together, regulating Hippo suggests new strategies for promoting cochlear supporting cell proliferation, HC regeneration, and reconnection with neurons in mammals.

Aminoglycoside antibiotics (AGAs) are widely used in life-threatening infections, but they accumulate in cochlear hair cells (HCs) and result in hearing loss. Increases in adenosine triphosphate (ATP) concentrations and P2X7 receptor expression were observed after neomycin treatment. Here, we demonstrated that P2X7 receptor, which is a non-selective cation channel that is activated by high ATP concentrations, may participate in the process through which AGAs enter hair cells. Using transgenic knockout mice, we found that P2X7 receptor deficiency protects HCs against neomycin-induced injury in vitro and in vivo. Subsequently, we used fluorescent gentamicin–Fluor 594 to study the uptake of AGAs and found fluorescence labeling in wild-type mice but not in P2rx7−/− mice in vitro. In addition, knocking-out P2rx7 did not significantly alter the HC count and auditory signal transduction, but it did inhibit mitochondria-dependent oxidative stress and apoptosis in the cochlea after neomycin exposure. We thus conclude that the P2X7 receptor may be linked to the entry of AGAs into HCs and is likely to be a therapeutic target for auditory HC protection.

Abstract Hearing loss is the most common sensory neural disorder in humans, and according to a WHO estimation, 5.5% (466 million) of people worldwide have disabling hearing loss. In this study, a Chinese family with prelingual sensorineural hearing loss was investigated. The affected individuals showed moderately severe hearing loss at all frequencies. Using target genome enrichment and high-throughput sequencing, the homozygous variant c.2372del; p.(Ser791fs) was identified in PDZD7. This variant lies in exon 15 of PDZD7 and results in a frame shift followed by an early stop codon. It is classified as pathogenic according to the ACMG/AMP guidelines and ClinGen specifications. Our study expands the pathogenic variant spectrum of PDZD7 and strengthens the clinical importance of this gene in patients with moderately severe hearing loss.

Finite difference methods are commonly used in the pricing of discretely monitored exotic options in the Black–Scholes framework, but they tend to converge slowly due to discontinuities contained in terminal conditions. We present an effective analytical modification to existing finite difference methods that greatly enhances their performance on discretely monitored options with non-smooth terminal conditions. We apply this modification to the popular Crank–Nicolson method and obtain highly accurate option pricing results with significantly reduced CPU cost. We also introduce an adaptive mesh refinement technique that further improves the computational speed of the modified finite difference method. The proposed method is especially useful for options with high monitoring frequencies, which are difficult to price using other existing methods. [ABSTRACT FROM AUTHOR]

The dispersibility of carbon nanotubes (CNTs) in ultrahigh-performance concrete (UHPC) under different preparation processes was investigated. The dispersion stability, dispersion degree of CNT aggregates, and absorbance of CNT dispersions were measured by static observation, microscope observation, and ultraviolet spectrophotometer. The results showed that compared with adding polycarboxylic acid superplasticizer (SP) after ultrasound, adding SP at the same time as ultrasonic CNT solution obtained a better dispersion of the CNT solution. When the mass ratio of SP to CNT was 1∶1 , the dispersion of CNT suspension was the best under 400-W ultrasonic power for 30 min. The flow properties, setting time, and compressive strength of CNT-modified UHPC also were studied. The experimental results show that the flow performance of CNT-UHPC decreases with the increase of CNT content. The condensation time of CNT-UHPC decreases with the increase of CNT content. The addition of 0.1% by weight CNT can improve the compressive strength of UHPC and refine the microstructure of UHPC. In conclusion, the study results can help to improve the dispersion and stability of carbon nanotubes in UHPC and effectively enhance the strength of UHPC adding carbon nanotubes in terms of working performance, mechanical strength, and microscopic morphology. [ABSTRACT FROM AUTHOR]

Two new 2-arylbenzo[b]furans (1-2) and ten known compounds (3-12) were identified from the 95% EtOH extract of the branches and leaves of Itea indochinensis for the first time. Their structures were determined mainly based on extensive analyses of UV, IR, 1D/2D NMR and HRMS spectra. The results of MTT assays demonstrated the anti-tumor potential of compound 1 with good selectivity, which displayed moderate inhibitory effects on proliferation of SK-hep-1 cells with IC50 value of 22.3 μM, while weak inhibitory effect on proliferation of HepG2 cells with an inhibition rate of 25% at 20 μM, and no obviously inhibitory effect on proliferation of A549 cells at 20 μM. In addition, compound 1 exhibited its significant scavenging capacity on ABTS·+ free radical with an IC50 value of 0.11 mg/mL, while weak scavenging effects on DPPH and O2·- radicals with scavenging ratios of 32.93% and 21.49% at 1 mg/mL, respectively. [ABSTRACT FROM AUTHOR]