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49 results on '"Luo, Dengjie"'

20. HcLEA113, a late embryogenesis abundant protein gene, positively regulates drought‐stress responses in kenaf.

Author

Luo, Dengjie, Wang, Caijin, Mubeen, Samavia, Rehman, Muzammal, Cao, Shan, Yue, Jiao, Pan, Jiao, Jin, Gang, Li, Ru, Chen, Tao, and Chen, Peng

Subjects
*GENE silencing, *REACTIVE oxygen species, *GENETIC engineering, *PLANT proteins, *HYDROGEN peroxide, *DROUGHT tolerance
Abstract

Late embryogenesis abundant (LEA) proteins have been widely recognized for their role in various abiotic stress responses in higher plants. Nevertheless, the specific mechanism responsible for the function of LEA proteins in plants has not yet been explored. This research involved the isolation and characterization of HcLEA113 from kenaf, revealing a significant increase in its expression in response to drought stress. When HcLEA113 was introduced into yeast, it resulted in an improved survival rate under drought conditions. Furthermore, the overexpression of HcLEA113 in tobacco plants led to enhanced tolerance to drought stress. Specifically, HcLEA113‐OE plants exhibited higher germination rates, longer root lengths, greater chlorophyll content, and higher relative water content under drought stress compared to wild‐type (WT) plants, while their relative conductivity was significantly lower than that of WT plants. Further physiological measurements revealed that the proline content, soluble sugars, and antioxidant activities of WT and HcLEA113‐OE tobacco leaves increased significantly under drought stress, with greater changes in HcLEA113‐OE plants than WT. The increase in hydrogen peroxide (H2O2), superoxide anions (O2−), and malondialdehyde (MDA) content was significantly lower in HcLEA113‐OE lines than in WT plants. Additionally, HcLEA113‐OE plants can activate reactive oxygen species (ROS)‐ and osmotic‐related genes in response to drought stress. On the other hand, silencing the HcLEA113 gene through virus‐induced gene silencing (VIGS) in kenaf plants led to notable growth suppression when exposed to drought conditions, manifesting as decreased plant height and dry weight. Meanwhile, antioxidant enzymes' activity significantly decreased and the ROS content increased. This study offers valuable insights for future research on the genetic engineering of drought resistance in plants. [ABSTRACT FROM AUTHOR]

Published
2024
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26. Physiological and DNA methylation analysis provides epigenetic insights into kenaf cadmium tolerance heterosis

Author

Luo, Dengjie, primary, Lu, Hai, additional, Wang, Caijin, additional, Mubeen, Samavia, additional, Cao, Shan, additional, Yue, Jiao, additional, Pan, Jiao, additional, Wu, Xia, additional, Wu, Qijing, additional, Zhang, Hui, additional, Chen, Canni, additional, Rehman, Muzammal, additional, Li, Ru, additional, and Chen, Peng, additional

Published
2023
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29. Functional characterization of a soybean GmSUT4 gene reveals its involvement in plant growth and development regulation through sugar metabolism

Author

Chen, Peng, primary, Wu, Xia, additional, Yue, Jiao, additional, Nie, Jingzhi, additional, Mubeen, Samavia, additional, Luo, Dengjie, additional, Cao, Shan, additional, Wang, Caijin, additional, Wu, Qijing, additional, Zhang, Hui, additional, Pan, Jiao, additional, Chen, Channi, additional, and Wang, Meng, additional

Published
2022
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30. Comparative Physiological and Transcriptomic Analysis Provide New Insights of Crucial Pathways and Genes Regulating Kenaf Salt Tolerance

Author

Hu, Yali, primary, Li, Zengqiang, additional, Tang, Meiqiong, additional, Luo, Dengjie, additional, Kashif, Muhammad Haneef, additional, Cao, Shan, additional, Wang, Caijin, additional, Yue, Jiao, additional, Huang, Zhen, additional, Pan, Jiao, additional, Wu, Xia, additional, Wu, Qijing, additional, Zhang, Hui, additional, Li, Ru, additional, and Chen, Peng, additional

Published
2022
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31. Methyl-Sensitive Amplification Polymorphism (MSAP) Analysis Provides Insights into the DNA Methylation Underlying Heterosis in Kenaf (Hibiscus Cannabinus L.) Drought Tolerance

Author

Luo, Dengjie, primary, Cao, Shan, additional, Li, Zengqiang, additional, Tang, Meiqiong, additional, Wang, Caijin, additional, Mackon, Enerand, additional, Huang, Zhen, additional, Pan, Jiao, additional, Wu, Xia, additional, Wu, Qijing, additional, Zhang, Hui, additional, Li, Renxue, additional, Li, Xin, additional, Li, Ru, additional, and Chen, Peng, additional

Published
2022
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32. Integrated Methylome and Transcriptome Analysis Provides Insights into the DNA Methylation Underlying the Mechanism of Cytoplasmic Male Sterility in Kenaf (Hibiscus cannabinus L.)

Author

Li, Zengqiang, primary, Luo, Dengjie, additional, Tang, Meiqiong, additional, Cao, Shan, additional, Pan, Jiao, additional, Zhang, Wenxian, additional, Hu, Yali, additional, Yue, Jiao, additional, Huang, Zhen, additional, Li, Ru, additional, and Chen, Peng, additional

Published
2022
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34. Physiological and Transcriptome Analysis Reveals Key Genes and Molecular Basis into Heterosis of Kenaf (Hibiscus Cannabinus L.) Under Drought Stress

Author

Luo, Dengjie, primary, Wang, Caijin, additional, Cao, Shan, additional, Mubeen, Samavia, additional, Mackon, Enerand, additional, Yue, Jiao, additional, Rehman, Muzammal, additional, Pan, Jiao, additional, Wu, Xia, additional, Wu, Qijing, additional, Zhang, Hui, additional, Chen, Tao, additional, Li, Ru, additional, and Chen, Peng, additional

Published
2022
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36. Physiological and DNA Methylation Analysis Provides Epigenetic Insights into Kenaf Cadmium Tolerance Heterosis

Author

Luo, Dengjie, primary, Lu, Hai, additional, Wang, Caijin, additional, Mubeen, Samavia, additional, Cao, Shan, additional, Yue, Jiao, additional, Pan, Jiao, additional, Wu, Xia, additional, Wu, Qijing, additional, Zhang, Hui, additional, Chen, Canni, additional, Rehman, Muzammal, additional, Li, Ru, additional, and Chen, Peng, additional

Published
2022
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40. 5-azacytidine pre-treatment alters DNA methylation levels and induces genes responsive to salt stress in kenaf (Hibiscus cannabinus L.)

Author

Li, Zengqiang, primary, Hu, Yali, additional, Chang, Mengmeng, additional, Kashif, Muhammad Haneef, additional, Tang, Meiqiong, additional, Luo, Dengjie, additional, Cao, Shan, additional, Lu, Hai, additional, Zhang, Wenxian, additional, Huang, Zhen, additional, Yue, Jiao, additional, and Chen, Peng, additional

Published
2021
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48. Screening of Broad-spectrum Resistance Resources against Rice Bacterial Leaf Streak.

Author

Hong Dengwei, Zhao Yan, Luo Dengjie, Shi Lijun, Li Rongbai, and Liu Fang

Subjects
RICE disease & pest resistance, RICE breeding, RICE varieties, HARVESTING, RICE, PLANT growth
Abstract

[Objectives] Rice materials with broad-spectrum resistance against several pathogenic strains of Xaruhomans oryzae pv. Oryzicola (Xooc) at multiple growth stages were screened, in order to provide reliable resistance sources for variety breeding against rice bacterial leaf streak. [Methods] A total of 1100 rice lines with rich genetic background were offered as selective objects, and highly susceptible cultivar Jingang 30 was used as the control. Inoculation was carried out with five strong pathogenic strains of Xooc through acupuncture method at various growth stages for resistance identification. [Results] Fourteen disease-resistant materials were obtained through preliminary screening, accounting for 1.27% of the total materials. Nine materials moderately resistant to bacterial leaf streak were obtained via secondary screening, accounting for 0.82% of the total materials. Besides, three of them (RL6, RL9 and RL14) were resistant to a number of pathogenic strains of Xooc at three growth stages. In particular, RL16 presented broad-spectrum resistance to multi-strains with high resistance levels. Six of them (RL2, RL4, RL5, RL8, RL11 and RL12) were resistant to single strain at single growth stage. [Conclusions] The obtained three materials have broad-spectrum resistance to bacterial leaf streak at multiple growth stages, which can be served as an important source to cultivate disease-resistant rice varieties. RL6 maintains high broad-spectrum resistance at all growth stages, which can be used as a prior resistance source for rice variety breeding. Six materials obtained with resistance against bacterial leaf streak at particular growth stages can be used as candidate resistance sources. [ABSTRACT FROM AUTHOR]

Published
2017

49. Integrated physiological and metabolomic responses reveal mechanisms of Cd tolerance and detoxification in kenaf ( Hibiscus cannabinus L.) under Cd stress.

Author

Saeed W, Mubeen S, Pan J, Rehman M, Fang W, Luo D, Liu P, Li Y, and Chen P

Abstract

Introduction: Cadmium (Cd) is a highly toxic trace element that occurs in large quantities in agricultural soils. The cultivation of industrial crops with high phytoremediation potential, such as kenaf, could effectively reduce soil Cd contamination, but the mechanisms of toxicity, tolerance, and detoxification remain unclear., Methods: In this study, the effects of different Cd concentrations (0, 100, 250, and 400 µM) on growth, biomass, Cd uptake, physiological parameters, metabolites and gene expression response of kenaf were investigated in a hydroponic experiment., Results and Discussion: The results showed that Cd stress significantly altered the ability of kenaf to accumulate and transport Cd; increased the activity of hydrogen peroxide (H 2 O 2 ), superoxide anion (O 2 - ), and malondialdehyde (MDA); reduced the activities of superoxide dismutase ( SOD) and catalase (CAT); and decreased the content of photosynthetic pigments, resulting in significant changes in growth and biomass production. Exposure to Cd was found to have a detrimental effect on the ascorbate-glutathione (AsA-GSH) cycle in the roots, whereas it resulted in an elevation in AsA levels and a reduction in GSH levels in the leaves. The increased content of cell wall polysaccharides under Cd stress could contribute to Cd retention in roots and limited Cd transport to above-ground plant tissues. Metabolomic analyses revealed that alanine, aspartate, and glutamate metabolism, oxidative phosphorylation, ABC transporter, and carbon metabolism were the major metabolic pathways associated with Cd stress tolerance. Cd stress increased gene expression of IRT1 and MTP1 in roots, which resulted in kenaf roots accumulating high Cd concentrations. This study extends our knowledge of the factors regulating the response of kenaf to Cd stress. This work provided a physiological and metabolomic perspective on the mechanism controlling the response of kenaf to Cd stress., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Saeed, Mubeen, Pan, Rehman, Fang, Luo, Liu, Li and Chen.)

Published
2024
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