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4 results on '"Lung Fibrocytes"'

1. Fibrocytes are increased in lung and peripheral blood of patients with idiopathic pulmonary fibrosis

Author

P. Heukels, J. A. C. van Hulst, M. van Nimwegen, C. E. Boorsma, B. N. Melgert, L. M. van den Toorn, K. A. T. Boomars, M. S. Wijsenbeek, H. Hoogsteden, J. H. von der Thüsen, R. W. Hendriks, M. Kool, and B. van den Blink

Subjects
Fibrocytes, Idiopathic pulmonary fibrosis, Lung Fibrocytes, Pulmonary hypertension, Flow cytometry, Diseases of the respiratory system, RC705-779
Abstract

Abstract Background Fibrocytes are implicated in Idiopathic Pulmonary Fibrosis (IPF) pathogenesis and increased proportions in the circulation are associated with poor prognosis. Upon tissue injury, fibrocytes migrate to the affected organ. In IPF patients, circulating fibrocytes are increased especially during exacerbations, however fibrocytes in the lungs have not been examined. Therefore, we sought to evaluate if fibrocytes can be detected in IPF lungs and we compare percentages and phenotypic characteristics of lung fibrocytes with circulating fibrocytes in IPF. Methods First we optimized flow cytometric detection circulating fibrocytes using a unique combination of intra- and extra-cellular markers to establish a solid gating strategy. Next we analyzed lung fibrocytes in single cell suspensions of explanted IPF and control lungs and compared characteristics and numbers with circulating fibrocytes of IPF. Results Using a gating strategy for both circulating and lung fibrocytes, which excludes potentially contaminating cell populations (e.g. neutrophils and different leukocyte subsets), we show that patients with IPF have increased proportions of fibrocytes, not only in the circulation, but also in explanted end-stage IPF lungs. These lung fibrocytes have increased surface expression of HLA-DR, increased intracellular collagen-1 expression, and also altered forward and side scatter characteristics compared with their circulating counterparts. Conclusions These findings demonstrate that lung fibrocytes in IPF patients can be quantified and characterized by flow cytometry. Lung fibrocytes have different characteristics than circulating fibrocytes and represent an intermediate cell population between circulating fibrocytes and lung fibroblast. Therefore, more insight in their phenotype might lead to specific therapeutic targeting in fibrotic lung diseases.

Published
2018
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3. Fibrocytes are increased in lung and peripheral blood of patients with idiopathic pulmonary fibrosis

Author

Heukels, P. (P.), van Hulst, J.A.C. (J. A.C.), Nimwegen, M. (Menno) van, Boorsma, C.E. (C. E.), Melgert, B.N. (B. N.), Toorn, L.M. (Leon) van den, Boomars, K.A.T. (Karin), Wijsenbeek-Lourens, M.S. (Marlies), Hoogsteden, H.C. (Henk), Thusen, J.H. (Jan) von der, Hendriks, R.W. (Rudi), Kool, M. (Mirjam), Blink, B. (Bernt) van den, Heukels, P. (P.), van Hulst, J.A.C. (J. A.C.), Nimwegen, M. (Menno) van, Boorsma, C.E. (C. E.), Melgert, B.N. (B. N.), Toorn, L.M. (Leon) van den, Boomars, K.A.T. (Karin), Wijsenbeek-Lourens, M.S. (Marlies), Hoogsteden, H.C. (Henk), Thusen, J.H. (Jan) von der, Hendriks, R.W. (Rudi), Kool, M. (Mirjam), and Blink, B. (Bernt) van den

Abstract

Background: Fibrocytes are implicated in Idiopathic Pulmonary Fibrosis (IPF) pathogenesis and increased proportions in the circulation are associated with poor prognosis. Upon tissue injury, fibrocytes migrate to the affected organ. In IPF patients, circulating fibrocytes are increased especially during exacerbations, however fibrocytes in the lungs have not been examined. Therefore, we sought to evaluate if fibrocytes can be detected in IPF lungs and we compare percentages and phenotypic characteristics of lung fibrocytes with circulating fibrocytes in IPF. Methods: First we optimized flow cytometric detection circulating fibrocytes using a unique combination of intra- and extra-cellular markers to establish a solid gating strategy. Next we analyzed lung fibrocytes in single cell suspensions of explanted IPF and control lungs and compared characteristics and numbers with circulating fibrocytes of IPF. Results: Using a gating strategy for both circulating and lung fibrocytes, which excludes potentially contaminating cell populations (e.g. neutrophils and different leukocyte subsets), we show that patients with IPF have increased proportions of fibrocytes, not only in the circulation, but also in explanted end-stage IPF lungs. These lung fibrocytes have increased surface expression of HLA-DR, increased intracellular collagen-1 expression, and also altered forward and side scatter characteristics compared with their circulating counterparts. Conclusions: These findings demonstrate that lung fibrocytes in IPF patients can be quantified and characterized by flow cytometry. Lung fibrocytes have different characteristics than circulating fibrocytes and represent an intermediate cell population between circulating fibrocytes and lung fibroblast. Therefore, more insight in their phenotype might lead to specific therapeutic targeting in fibrotic lung diseases.

Published
2018
Full Text
View/download PDF

4. Fibrocytes are increased in lung and peripheral blood of patients with idiopathic pulmonary fibrosis

Author

J. Von Der Thüsen, Peter Heukels, Mirjam Kool, Carian E. Boorsma, M. van Nimwegen, J. A. C. van Hulst, Marlies S. Wijsenbeek, K. A. Boomars, L M van den Toorn, B. van den Blink, Henk C. Hoogsteden, Barbro N. Melgert, Rudolf W. Hendriks, Nanomedicine & Drug Targeting, Groningen Research Institute for Asthma and COPD (GRIAC), Biopharmaceuticals, Discovery, Design and Delivery (BDDD), Pulmonary Medicine, and Pathology

Subjects
0301 basic medicine, Male, Pathology, medicine.medical_specialty, MIGRATION, Population, Idiopathic pulmonary fibrosis, Pulmonary hypertension, Pathogenesis, 03 medical and health sciences, 0302 clinical medicine, INFLAMMATION, Fibrocyte, medicine, TISSUE-REPAIR, Humans, Flow cytometry, Fibroblast, education, Lung, Cells, Cultured, NEUTROPHILS, lcsh:RC705-779, education.field_of_study, CIRCULATING FIBROCYTES, IDENTIFICATION, business.industry, TRANSPLANTATION, Research, Lung Fibrocytes, Fibrocytes, PNEUMONITIS, lcsh:Diseases of the respiratory system, Fibroblasts, respiratory system, medicine.disease, respiratory tract diseases, Transplantation, 030104 developmental biology, medicine.anatomical_structure, DIFFERENTIATION, 030228 respiratory system, CELLS, Leukocytes, Mononuclear, Female, business
Abstract

Background Fibrocytes are implicated in Idiopathic Pulmonary Fibrosis (IPF) pathogenesis and increased proportions in the circulation are associated with poor prognosis. Upon tissue injury, fibrocytes migrate to the affected organ. In IPF patients, circulating fibrocytes are increased especially during exacerbations, however fibrocytes in the lungs have not been examined. Therefore, we sought to evaluate if fibrocytes can be detected in IPF lungs and we compare percentages and phenotypic characteristics of lung fibrocytes with circulating fibrocytes in IPF. Methods First we optimized flow cytometric detection circulating fibrocytes using a unique combination of intra- and extra-cellular markers to establish a solid gating strategy. Next we analyzed lung fibrocytes in single cell suspensions of explanted IPF and control lungs and compared characteristics and numbers with circulating fibrocytes of IPF. Results Using a gating strategy for both circulating and lung fibrocytes, which excludes potentially contaminating cell populations (e.g. neutrophils and different leukocyte subsets), we show that patients with IPF have increased proportions of fibrocytes, not only in the circulation, but also in explanted end-stage IPF lungs. These lung fibrocytes have increased surface expression of HLA-DR, increased intracellular collagen-1 expression, and also altered forward and side scatter characteristics compared with their circulating counterparts. Conclusions These findings demonstrate that lung fibrocytes in IPF patients can be quantified and characterized by flow cytometry. Lung fibrocytes have different characteristics than circulating fibrocytes and represent an intermediate cell population between circulating fibrocytes and lung fibroblast. Therefore, more insight in their phenotype might lead to specific therapeutic targeting in fibrotic lung diseases. Electronic supplementary material The online version of this article (10.1186/s12931-018-0798-8) contains supplementary material, which is available to authorized users.

Published
2018

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