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14 results on '"Lunardi, Franciele Osmarini"'

3. Criopreserved ovarian tissue transplantation and restoration of bone metabolism in castrated rats

Author

Silva, Lígia Helena Ferreira Melo e, Silva, João Marcos de Meneses e, Salama, Mahmoud, Pinheiro, Luiz Gonzaga Porto, Lunardi, Franciele Osmarini, Silva, Paulo Goberlânio Barros da, Hirth, Carlos Gustavo, Lucena, Igor Freitas de, Gomes, Glauco Jorge da Costa, and Leite, José Alberto Dias

Subjects
Cryopreservation, Osteoporose Pós-Menopausa, Estrogênios, Tissue Transplantation, Insuficiência Ovariana Primária, Estrogens, Menopause, Primary Ovarian Insufficiency, Osteoporosis, Postmenopausal, Menopausa
Abstract

Objectives: to evaluate estradiol levels and autotransplantation heated ovarian tissue effects, after vitrification, on rats bone metabolism previously oophorectomized bilaterally. Methods: experimental study with 27 rats aged 11 to 12 weeks and weighing 200g to 300g, submitted to bilateral oophorectomy and ovarian tissue cryopreservation for subsequent reimplantation. Animals were divided into two groups, A and B, with 8 and 19 rats, respectively. Autotransplantation occurred in two periods according to castration time: after one week, in group A, and after one month in group B. Serum estradiol measurements and ovary and tibia histological analysis were performed before and after oophorectomy period (early or late) and one month after reimplantation. Results: in groups A and B, tibia median cortical thickness was 0.463±0.14mm (mean±SD) at the baseline, 0.360±0.14mm after oophorectomy and 0.445±0.17mm one month after reimplantation p

Published
2018

4. Criopreserved ovarian tissue transplantation and bone restoration metabolism in castrated rats

Author

SILVA, LÍGIA HELENA FERREIRA MELO E, primary, SILVA, JOÃO MARCOS DE MENESES E, additional, SALAMA, MAHMOUD, additional, PINHEIRO, LUIZ GONZAGA PORTO, additional, LUNARDI, FRANCIELE OSMARINI, additional, SILVA, PAULO GOBERLÂNIO BARROS DA, additional, HIRTH, CARLOS GUSTAVO, additional, LUCENA, IGOR FREITAS DE, additional, GOMES, GLAUCO JORGE DA COSTA, additional, and LEITE, JOSÉ ALBERTO DIAS, additional

Published
2018
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6. Sheep Isolated Secondary Follicles Are Able to Produce Metaphase II Oocytes After Vitrification and Long-TermIn VitroGrowth

Author

Lunardi, Franciele Osmarini, primary, de Aguiar, Francisco Leo Nascimento, additional, Apolloni, Livia Brunetti, additional, Duarte, Ana Beatriz Graça, additional, de Sá, Naiza Arcângela Ribeiro, additional, Leal, Érica Suzanne Soares, additional, Sales, Antonia Debora, additional, Lobo, Carlos Henrique, additional, Campello, Cláudio Cabral, additional, Smitz, Johan, additional, Apgar, Gary Allen, additional, de Figueiredo, José Ricardo, additional, and Rodrigues, Ana Paula Ribeiro, additional

Published
2017
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7. Connexin 37 and 43 gene and protein expression and developmental competence of isolated ovine secondary follicles cultured in vitro after vitrification of ovarian tissue

Author

Sampaio da Silva, Andréa Moreira, primary, Bruno, Jamily Bezerra, additional, de Lima, Laritza Ferreira, additional, Ribeiro de Sá, Naíza Arcângela, additional, Lunardi, Franciele Osmarini, additional, Ferreira, Anna Clara Accioly, additional, Vieira Correia, Hudson Henrique, additional, de Aguiar, Francisco Léo Nascimento, additional, Araújo, Valdevane Rocha, additional, Lobo, Carlos Henrique, additional, de Alencar Araripe Moura, Arlindo, additional, Campello, Cláudio Cabral, additional, Smitz, Johan, additional, de Figueiredo, José Ricardo, additional, and Ribeiro Rodrigues, Ana Paula, additional

Published
2016
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8. Ovine secondary follicles vitrified out the ovarian tissue grow and develop in vitro better than those vitrified into the ovarian fragments

Author

Lunardi, Franciele Osmarini, primary, de Aguiar, Francisco Leo Nascimento, additional, Duarte, Ana Beatriz Graça, additional, Araújo, Valdevane Rocha, additional, de Lima, Laritza Ferreira, additional, Ribeiro de Sá, Naiza Arcângela, additional, Vieira Correia, Hudson Henrique, additional, Domingues, Sheyla Farhayldes Souza, additional, Campello, Cláudio Cabral, additional, Smitz, Johan, additional, de Figueiredo, José Ricardo, additional, and Ribeiro Rodrigues, Ana Paula, additional

Published
2016
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10. Sheep Isolated Secondary Follicles Are Able to Produce Metaphase II Oocytes After Vitrification and Long-Term In Vitro Growth.

Author

Lunardi, Franciele Osmarini, de Aguiar, Francisco Leo Nascimento, Apolloni, Livia Brunetti, Duarte, Ana Beatriz Graça, de Sá, Naiza Arcângela Ribeiro, Leal, Érica Suzanne Soares, Sales, Antonia Debora, Lobo, Carlos Henrique, Campello, Cláudio Cabral, Smitz, Johan, Apgar, Gary Allen, de Figueiredo, José Ricardo, and Rodrigues, Ana Paula Ribeiro

Abstract

The vitrification of preantral follicles followed by in vitro growth (IVG) could be valuable to produce fertilizable oocytes. However, the meiotic resumption rates of oocytes cultured from vitrified secondary follicles (SF) have been reported as suboptimal. This study aimed to verify two base media (alpha modification of minimum essential medium, α-MEM, and tissue culture medium 199, TCM199) on vitrified SF regarding different requirements during IVG. Sheep ovarian fragments were divided in six groups: (1) Fresh groups (Control α-MEM and TCM199): SF without vitrification; (2) Follicle-Vitrified (Follicle-Vit α-MEM and TCM199): SF vitrified after isolation; and (3) Tissue-Vitrified (Tissue-Vit α-MEM and TCM199): SF vitrified enclosed in ovarian fragments and, subsequently, isolated. The isolated SF were submitted to IVG for 18 days. Thereafter, the recovered cumulus-oocyte complexes (COCs) underwent in vitro maturation (IVM) and evaluation of chromatin configuration. Follicular granulosa cells were analyzed for their gene expression of Bax, Bcl2, and Connexins ( CX) 37 and 43. COCs from in vivo antral follicles were used as in vivo control. Data were analyzed by analysis of variance, Tukey, and chi-square tests. Differences were considered significant if p-value is <0.05. Follicle-Vit groups had higher ( p < 0.05) percentage of antrum formation compared with Tissue-Vit groups. Vitrification did not affect ( p > 0.05) oocyte diameter postmaturation. Oocytes from Follicle-Vit in α-MEM reached metaphase II stage after IVM. Gene expression for CX37, CX43, and Bax was lower in Tissue-Vit groups. For Bcl2, the gene expression was the opposite. In conclusion, during IVG for 18 days, maximal oocyte meiotic resumption was not negatively impacted by vitrification and was greatest for isolated SF using α-MEM as a medium. [ABSTRACT FROM AUTHOR]

Published
2017
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13. Morphologic, viability and ultrastructural analysis of vitrified sheep preantral follicles enclosed in ovarian tissue

Author

Lunardi, Franciele Osmarini, primary, Araújo, Valdevane Rocha, additional, Faustino, Luciana Rocha, additional, Carvalho, Adeline de Andrade, additional, Gonçalves, Raphael Fernando Braga, additional, Bass, Casie Shantel, additional, Báo, Sônia Nair, additional, Name, Khesller Patrícia Olázia, additional, Campello, Cláudio Cabral, additional, de Figueiredo, José Ricardo, additional, and Rodrigues, Ana Paula Ribeiro, additional

Published
2012
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14. Restoring fertility after ovarian tissue cryopreservation: a half century of research.

Author

Lunardi, Franciele Osmarini, Araújo, Valdevane Rocha, Bernuci, Marcelo Picinin, Lunardi, Luciane Osmarini, Gonçalves, Raphael Fernando Braga, Carvalho, Adeline de Andrade, de Figueiredo, José Ricardo, and Rodrigues, Ana Paula Ribeiro

Abstract

Tissue transplantation and in vitro ovarian follicle culture have been investigated as alternative techniques to restore fertility in young women who are facing fertility-threatening diseases or treatments following ovarian tissue cryopreservation. Although transplants of fresh or frozen ovarian tissue have successfully yielded healthy live births in different species including humans, the risks of reintroducing cancer cells back into the patient, post treatment, have limited its clinical purpose. The in vitro ovarian follicle culture minimizes these risks and provides a way to harvest more mature oocytes, however its clinical translation has yet to be determined. Not only is it possible for tissue cryopreservation to safeguard fertility in cancer patients, this technique also allows the maintenance of germplasm banks for animals of high commercial value or for those animals that are at risk of extinction. Given the importance of managing female genetic material, this paper reviews the progress of the methods used to preserve and restore female fertility in different species to demonstrate the results obtained in the past 50 years of research, the current achievements and the future directions on this field. [ABSTRACT FROM PUBLISHER]

Published
2013
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