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318 results on '"Luna, Gabriel"'

1. Amelioration of Photoreceptor Degeneration by Intravitreal Transplantation of Retinal Progenitor Cells in Rats.

Author

Yang, Jing, Lewis, Geoffrey, Hsiang, Chin-Hui, Menges, Steven, Luna, Gabriel, Cho, William, Turovets, Nikolay, Fisher, Steven, and Klassen, Henry

Subjects
electroretinogram, intraocular injection, neuroprotection, retinal dystrophy, stem cells, Animals, Rats, Retinal Degeneration, Stem Cell Transplantation, Humans, Retina, Stem Cells, Photoreceptor Cells, Vertebrate, Disease Models, Animal
Abstract

Photoreceptor degeneration is a major cause of untreatable blindness worldwide and has recently been targeted by emerging technologies, including cell- and gene-based therapies. Cell types of neural lineage have shown promise for replacing either photoreceptors or retinal pigment epithelial cells following delivery to the subretinal space, while cells of bone marrow lineage have been tested for retinal trophic effects following delivery to the vitreous cavity. Here we explore an alternate approach in which cells from the immature neural retinal are delivered to the vitreous cavity with the goal of providing trophic support for degenerating photoreceptors. Rat and human retinal progenitor cells were transplanted to the vitreous of rats with a well-studied photoreceptor dystrophy, resulting in substantial anatomical preservation and functional rescue of vision. This work provides scientific proof-of-principle for a novel therapeutic approach to photoreceptor degeneration that is currently being evaluated in clinical trials.

Published
2024

3. Three-Dimensional Ultrastructure of the Normal Rod Photoreceptor Synapse and Degenerative Changes Induced by Retinal Detachment.

Author

Torten, Gil, Fisher, Steven, Linberg, Kenneth, Luna, Gabriel, Ellisman, Mark, Williams, David, and Perkins, Guy

Subjects
electron microscope tomography, photoreceptor, retinal detachment, rod degeneration, rod spherule, synapse, Female, Animals, Cats, Retinal Detachment, Microscopy, Electron, Synapses, Retina, Retinal Bipolar Cells, Retinal Rod Photoreceptor Cells, Mammals
Abstract

The rod photoreceptor synapse is the first synapse of dim-light vision and one of the most complex in the mammalian CNS. The components of its unique structure, a presynaptic ribbon and a single synaptic invagination enclosing several postsynaptic processes, have been identified, but disagreements about their organization remain. Here, we have used EM tomography to generate high-resolution images of 3-D volumes of the rod synapse from the female domestic cat. We have resolved the synaptic ribbon as a single structure, with a single arciform density, indicating the presence of one long site of transmitter release. The organization of the postsynaptic processes, which has been difficult to resolve with past methods, appears as a tetrad arrangement of two horizontal cell and two rod bipolar cell processes. Retinal detachment severely disrupts this organization. After 7 d, EM tomography reveals withdrawal of rod bipolar dendrites from most spherules; fragmentation of synaptic ribbons, which lose their tight association with the presynaptic membrane; and loss of the highly branched telodendria of the horizontal cell axon terminals. After detachment, the hilus, the opening through which postsynaptic processes enter the invagination, enlarges, exposing the normally sequestered environment within the invagination to the extracellular space of the outer plexiform layer. Our use of EM tomography provides the most accurate description to date of the complex rod synapse and details changes it undergoes during outer segment degeneration. These changes would be expected to disrupt the flow of information in the rod pathway.SIGNIFICANCE STATEMENT Ribbon-type synapses transmit the first electrical signals of vision and hearing. Despite their crucial role in sensory physiology, the three-dimensional ultrastructure of these synapses, especially the complex organization of the rod photoreceptor synapse, is not well understood. We used EM tomography to obtain 3-D imaging at nanoscale resolution to help resolve the organization of rod synapses in normal and detached retinas. This approach has enabled us to show that in the normal retina a single ribbon and arciform density oppose a tetrad of postsynaptic processes. In addition, it enabled us to provide a 3-D perspective of the ultrastructural changes that occur in response to retinal detachment.

Published
2023

4. Human tau mutations in cerebral organoids induce a progressive dyshomeostasis of cholesterol

Author

Glasauer, Stella MK, Goderie, Susan K, Rauch, Jennifer N, Guzman, Elmer, Audouard, Morgane, Bertucci, Taylor, Joy, Shona, Rommelfanger, Emma, Luna, Gabriel, Keane-Rivera, Erica, Lotz, Steven, Borden, Susan, Armando, Aaron M, Quehenberger, Oswald, Temple, Sally, and Kosik, Kenneth S

Subjects
Biochemistry and Cell Biology, Bioinformatics and Computational Biology, Genetics, Biological Sciences, Acquired Cognitive Impairment, Dementia, Stem Cell Research, Aging, Neurosciences, Stem Cell Research - Induced Pluripotent Stem Cell, Brain Disorders, Alzheimer's Disease Related Dementias (ADRD), Rare Diseases, Human Genome, Neurodegenerative, Alzheimer's Disease including Alzheimer's Disease Related Dementias (AD/ADRD), Alzheimer's Disease, Frontotemporal Dementia (FTD), Stem Cell Research - Induced Pluripotent Stem Cell - Human, Aetiology, 2.1 Biological and endogenous factors, Neurological, Cholesterol, Frontotemporal Dementia, Humans, Mutation, Organoids, tau Proteins, MAPT mutation, astrocyte, brain organoid, cholesterol, disease model, neurodegeneration, single-cell RNA sequencing, tau, tauopathy, Clinical Sciences, Biochemistry and cell biology
Abstract

Mutations in the MAPT gene that encodes tau lead to frontotemporal dementia (FTD) with pathology evident in both cerebral neurons and glia. Human cerebral organoids (hCOs) from individuals harboring pathogenic tau mutations can reveal the earliest downstream effects on molecular pathways within a developmental context, generating interacting neurons and glia. We found that in hCOs carrying the V337M and R406W tau mutations, the cholesterol biosynthesis pathway in astrocytes was the top upregulated gene set compared with isogenic controls by single-cell RNA sequencing (scRNA-seq). The 15 upregulated genes included HMGCR, ACAT2, STARD4, LDLR, and SREBF2. This result was confirmed in a homozygous R406W mutant cell line by immunostaining and sterol measurements. Cholesterol abundance in the brain is tightly regulated by efflux and cholesterol biosynthetic enzyme levels in astrocytes, and dysregulation can cause aberrant phosphorylation of tau. Our findings suggest that cholesterol dyshomeostasis is an early event in the etiology of neurodegeneration caused by tau mutations.

Published
2022

6. Long-term transverse imaging of the hippocampus with glass microperiscopes

Author

Redman, William T, Wolcott, Nora S, Montelisciani, Luca, Luna, Gabriel, Marks, Tyler D, Sit, Kevin K, Yu, Che-Hang, Smith, Spencer, and Goard, Michael J

Subjects
Biomedical and Clinical Sciences, Neurosciences, Mental Health, Biomedical Imaging, Neurological, Animals, Dendrites, Hippocampus, Mice, Neurons, Pyramidal Cells, hippocampus, two-photon imaging, place cell, Mouse, mouse, neuroscience, Biochemistry and Cell Biology, Biological sciences, Biomedical and clinical sciences, Health sciences
Abstract

The hippocampus consists of a stereotyped neuronal circuit repeated along the septal-temporal axis. This transverse circuit contains distinct subfields with stereotyped connectivity that support crucial cognitive processes, including episodic and spatial memory. However, comprehensive measurements across the transverse hippocampal circuit in vivo are intractable with existing techniques. Here, we developed an approach for two-photon imaging of the transverse hippocampal plane in awake mice via implanted glass microperiscopes, allowing optical access to the major hippocampal subfields and to the dendritic arbor of pyramidal neurons. Using this approach, we tracked dendritic morphological dynamics on CA1 apical dendrites and characterized spine turnover. We then used calcium imaging to quantify the prevalence of place and speed cells across subfields. Finally, we measured the anatomical distribution of spatial information, finding a non-uniform distribution of spatial selectivity along the DG-to-CA1 axis. This approach extends the existing toolbox for structural and functional measurements of hippocampal circuitry.

Published
2022

7. Functional neuronal circuitry and oscillatory dynamics in human brain organoids

Author

Sharf, Tal, van der Molen, Tjitse, Glasauer, Stella MK, Guzman, Elmer, Buccino, Alessio P, Luna, Gabriel, Cheng, Zhuowei, Audouard, Morgane, Ranasinghe, Kamalini G, Kudo, Kiwamu, Nagarajan, Srikantan S, Tovar, Kenneth R, Petzold, Linda R, Hierlemann, Andreas, Hansma, Paul K, and Kosik, Kenneth S

Subjects
Biomedical and Clinical Sciences, Engineering, Biomedical Engineering, Neurosciences, Physical Sciences, Stem Cell Research, Brain Disorders, Mental Health, Stem Cell Research - Induced Pluripotent Stem Cell, Stem Cell Research - Induced Pluripotent Stem Cell - Human, 1.1 Normal biological development and functioning, Underpinning research, Neurological, Brain, Humans, Induced Pluripotent Stem Cells, Microelectrodes, Neurons, Organoids
Abstract

Human brain organoids replicate much of the cellular diversity and developmental anatomy of the human brain. However, the physiology of neuronal circuits within organoids remains under-explored. With high-density CMOS microelectrode arrays and shank electrodes, we captured spontaneous extracellular activity from brain organoids derived from human induced pluripotent stem cells. We inferred functional connectivity from spike timing, revealing a large number of weak connections within a skeleton of significantly fewer strong connections. A benzodiazepine increased the uniformity of firing patterns and decreased the relative fraction of weakly connected edges. Our analysis of the local field potential demonstrate that brain organoids contain neuronal assemblies of sufficient size and functional connectivity to co-activate and generate field potentials from their collective transmembrane currents that phase-lock to spiking activity. These results point to the potential of brain organoids for the study of neuropsychiatric diseases, drug action, and the effects of external stimuli upon neuronal networks.

Published
2022

8. Organic synthesis of 1,2-dipalmitoyl-rac-glycero-3-phosphatidylethanolamine and its effect on the induction of apoptosis in normal human lung fibroblasts

Author

Tlatelpa-Romero, Beatriz, Contreras-Cruz, David Atahualpa, Guerrero-Luna, Gabriel, Hernández-Linares, María Guadalupe, Ruiz-Salgado, Sinuhé, Mendoza-Milla, Criselda, Romero, Yair, de-la-Rosa Paredes, René, Oyarzábal, Luis F., Mendoza-Sámano, Diego Alejandro, Galván-León, Jiovani Alfredo, and Vázquez-de-Lara, Luis G.

Published
2023
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9. LRP1 is a master regulator of tau uptake and spread

Author

Rauch, Jennifer N, Luna, Gabriel, Guzman, Elmer, Audouard, Morgane, Challis, Collin, Sibih, Youssef E, Leshuk, Carolina, Hernandez, Israel, Wegmann, Susanne, Hyman, Bradley T, Gradinaru, Viviana, Kampmann, Martin, and Kosik, Kenneth S

Subjects
Biochemistry and Cell Biology, Biological Sciences, Brain Disorders, Emerging Infectious Diseases, Neurosciences, Alzheimer's Disease, Dementia, Alzheimer's Disease including Alzheimer's Disease Related Dementias (AD/ADRD), Frontotemporal Dementia (FTD), Acquired Cognitive Impairment, Neurodegenerative, Aging, Alzheimer's Disease Related Dementias (ADRD), 2.1 Biological and endogenous factors, Aetiology, Neurological, Animals, Cell Line, Endocytosis, Female, Humans, Ligands, Low Density Lipoprotein Receptor-Related Protein-1, Male, Mice, Neurons, tau Proteins, General Science & Technology
Abstract

The spread of protein aggregates during disease progression is a common theme underlying many neurodegenerative diseases. The microtubule-associated protein tau has a central role in the pathogenesis of several forms of dementia known as tauopathies-including Alzheimer's disease, frontotemporal dementia and chronic traumatic encephalopathy1. Progression of these diseases is characterized by the sequential spread and deposition of protein aggregates in a predictable pattern that correlates with clinical severity2. This observation and complementary experimental studies3,4 have suggested that tau can spread in a prion-like manner, by passing to naive cells in which it templates misfolding and aggregation. However, although the propagation of tau has been extensively studied, the underlying cellular mechanisms remain poorly understood. Here we show that the low-density lipoprotein receptor-related protein 1 (LRP1) controls the endocytosis of tau and its subsequent spread. Knockdown of LRP1 significantly reduced tau uptake in H4 neuroglioma cells and in induced pluripotent stem cell-derived neurons. The interaction between tau and LRP1 is mediated by lysine residues in the microtubule-binding repeat region of tau. Furthermore, downregulation of LRP1 in an in vivo mouse model of tau spread was found to effectively reduce the propagation of tau between neurons. Our results identify LRP1 as a key regulator of tau spread in the brain, and therefore a potential target for the treatment of diseases that involve tau spread and aggregation.

Published
2020

11. A farnesyltransferase inhibitor activates lysosomes and reduces tau pathology in mice with tauopathy

Author

Hernandez, Israel, Luna, Gabriel, Rauch, Jennifer N, Reis, Surya A, Giroux, Michel, Karch, Celeste M, Boctor, Daniel, Sibih, Youssef E, Storm, Nadia J, Diaz, Antonio, Kaushik, Susmita, Zekanowski, Cezary, Kang, Alexander A, Hinman, Cassidy R, Cerovac, Vesna, Guzman, Elmer, Zhou, Honjun, Haggarty, Stephen J, Goate, Alison M, Fisher, Steven K, Cuervo, Ana M, and Kosik, Kenneth S

Subjects
Neurosciences, Neurodegenerative, Rare Diseases, Frontotemporal Dementia (FTD), Alzheimer's Disease, Brain Disorders, Alzheimer's Disease including Alzheimer's Disease Related Dementias (AD/ADRD), Aging, Alzheimer's Disease Related Dementias (ADRD), Acquired Cognitive Impairment, Dementia, Development of treatments and therapeutic interventions, Aetiology, 5.1 Pharmaceuticals, 2.1 Biological and endogenous factors, Neurological, Animals, Brain, Disease Models, Animal, Enzyme Inhibitors, Farnesyltranstransferase, Female, GTP-Binding Proteins, Humans, Induced Pluripotent Stem Cells, Lysosomes, Male, Mice, Mice, Transgenic, Mutation, Neurons, Piperidines, Proteolysis, Pyridines, RNA, Small Interfering, Tauopathies, Translational Research, Biomedical, tau Proteins, Biological Sciences, Medical and Health Sciences
Abstract

Tau inclusions are a shared feature of many neurodegenerative diseases, among them frontotemporal dementia caused by tau mutations. Treatment approaches for these conditions include targeting posttranslational modifications of tau proteins, maintaining a steady-state amount of tau, and preventing its tendency to aggregate. We discovered a new regulatory pathway for tau degradation that operates through the farnesylated protein, Rhes, a GTPase in the Ras family. Here, we show that treatment with the farnesyltransferase inhibitor lonafarnib reduced Rhes and decreased brain atrophy, tau inclusions, tau sumoylation, and tau ubiquitination in the rTg4510 mouse model of tauopathy. In addition, lonafarnib treatment attenuated behavioral abnormalities in rTg4510 mice and reduced microgliosis in mouse brain. Direct reduction of Rhes in the rTg4510 mouse by siRNA reproduced the results observed with lonafarnib treatment. The mechanism of lonafarnib action mediated by Rhes to reduce tau pathology was shown to operate through activation of lysosomes. We finally showed in mouse brain and in human induced pluripotent stem cell-derived neurons a normal developmental increase in Rhes that was initially suppressed by tau mutations. The known safety of lonafarnib revealed in human clinical trials for cancer suggests that this drug could be repurposed for treating tauopathies.

Published
2019

12. 2021 Taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales

Author

Kuhn, Jens H., Adkins, Scott, Agwanda, Bernard R., Al Kubrusli, Rim, Alkhovsky, Sergey V., Amarasinghe, Gaya K., Avšič-Županc, Tatjana, Ayllón, María A., Bahl, Justin, Balkema-Buschmann, Anne, Ballinger, Matthew J., Basler, Christopher F., Bavari, Sina, Beer, Martin, Bejerman, Nicolas, Bennett, Andrew J., Bente, Dennis A., Bergeron, Éric, Bird, Brian H., Blair, Carol D., Blasdell, Kim R., Blystad, Dag-Ragnar, Bojko, Jamie, Borth, Wayne B., Bradfute, Steven, Breyta, Rachel, Briese, Thomas, Brown, Paul A., Brown, Judith K., Buchholz, Ursula J., Buchmeier, Michael J., Bukreyev, Alexander, Burt, Felicity, Büttner, Carmen, Calisher, Charles H., Cao, Mengji, Casas, Inmaculada, Chandran, Kartik, Charrel, Rémi N., Cheng, Qi, Chiaki, Yuya, Chiapello, Marco, Choi, Il-Ryong, Ciuffo, Marina, Clegg, J. Christopher S., Crozier, Ian, Dal Bó, Elena, de la Torre, Juan Carlos, de Lamballerie, Xavier, de Swart, Rik L., Debat, Humberto, Dheilly, Nolwenn M., Di Cicco, Emiliano, Di Paola, Nicholas, Di Serio, Francesco, Dietzgen, Ralf G., Digiaro, Michele, Dolnik, Olga, Drebot, Michael A., Drexler, J. Felix, Dundon, William G., Duprex, W. Paul, Dürrwald, Ralf, Dye, John M., Easton, Andrew J., Ebihara, Hideki, Elbeaino, Toufic, Ergünay, Koray, Ferguson, Hugh W., Fooks, Anthony R., Forgia, Marco, Formenty, Pierre B. H., Fránová, Jana, Freitas-Astúa, Juliana, Fu, Jingjing, Fürl, Stephanie, Gago-Zachert, Selma, Gāo, George Fú, García, María Laura, García-Sastre, Adolfo, Garrison, Aura R., Gaskin, Thomas, Gonzalez, Jean-Paul J., Griffiths, Anthony, Goldberg, Tony L., Groschup, Martin H., Günther, Stephan, Hall, Roy A., Hammond, John, Han, Tong, Hepojoki, Jussi, Hewson, Roger, Hong, Jiang, Hong, Ni, Hongo, Seiji, Horie, Masayuki, Hu, John S., Hu, Tao, Hughes, Holly R., Hüttner, Florian, Hyndman, Timothy H., Ilyas, M., Jalkanen, Risto, Jiāng, Dàohóng, Jonson, Gilda B., Junglen, Sandra, Kadono, Fujio, Kaukinen, Karia H., Kawate, Michael, Klempa, Boris, Klingström, Jonas, Kobinger, Gary, Koloniuk, Igor, Kondō, Hideki, Koonin, Eugene V., Krupovic, Mart, Kubota, Kenji, Kurath, Gael, Laenen, Lies, Lambert, Amy J., Langevin, Stanley L., Lee, Benhur, Lefkowitz, Elliot J., Leroy, Eric M., Li, Shaorong, Li, Longhui, Lǐ, Jiànróng, Liu, Huazhen, Lukashevich, Igor S., Maes, Piet, de Souza, William Marciel, Marklewitz, Marco, Marshall, Sergio H., Marzano, Shin-Yi L., Massart, Sebastien, McCauley, John W., Melzer, Michael, Mielke-Ehret, Nicole, Miller, Kristina M., Ming, Tobi J., Mirazimi, Ali, Mordecai, Gideon J., Mühlbach, Hans-Peter, Mühlberger, Elke, Naidu, Rayapati, Natsuaki, Tomohide, Navarro, José A., Netesov, Sergey V., Neumann, Gabriele, Nowotny, Norbert, Nunes, Márcio R. T., Olmedo-Velarde, Alejandro, Palacios, Gustavo, Pallás, Vicente, Pályi, Bernadett, Papa, Anna, Paraskevopoulou, Sofia, Park, Adam C., Parrish, Colin R., Patterson, David A., Pauvolid-Corrêa, Alex, Pawęska, Janusz T., Payne, Susan, Peracchio, Carlotta, Pérez, Daniel R., Postler, Thomas S., Qi, Liying, Radoshitzky, Sheli R., Resende, Renato O., Reyes, Carina A., Rima, Bertus K., Luna, Gabriel Robles, Romanowski, Víctor, Rota, Paul, Rubbenstroth, Dennis, Rubino, Luisa, Runstadler, Jonathan A., Sabanadzovic, Sead, Sall, Amadou Alpha, Salvato, Maria S., Sang, Rosemary, Sasaya, Takahide, Schulze, Angela D., Schwemmle, Martin, Shi, Mang, Shí, Xiǎohóng, Shí, Zhènglì, Shimomoto, Yoshifumi, Shirako, Yukio, Siddell, Stuart G., Simmonds, Peter, Sironi, Manuela, Smagghe, Guy, Smither, Sophie, Song, Jin-Won, Spann, Kirsten, Spengler, Jessica R., Stenglein, Mark D., Stone, David M., Sugano, Jari, Suttle, Curtis A., Tabata, Amy, Takada, Ayato, Takeuchi, Shigeharu, Tchouassi, David P., Teffer, Amy, Tesh, Robert B., Thornburg, Natalie J., Tomitaka, Yasuhiro, Tomonaga, Keizō, Tordo, Noël, Torto, Baldwyn, Towner, Jonathan S., Tsuda, Shinya, Tu, Changchun, Turina, Massimo, Tzanetakis, Ioannis E., Uchida, Janice, Usugi, Tomio, Vaira, Anna Maria, Vallino, Marta, van den Hoogen, Bernadette, Varsani, Arvind, Vasilakis, Nikos, Verbeek, Martin, von Bargen, Susanne, Wada, Jiro, Wahl, Victoria, Walker, Peter J., Wang, Lin-Fa, Wang, Guoping, Wang, Yanxiang, Wang, Yaqin, Waqas, Muhammad, Wèi, Tàiyún, Wen, Shaohua, Whitfield, Anna E., Williams, John V., Wolf, Yuri I., Wu, Jiangxiang, Xu, Lei, Yanagisawa, Hironobu, Yang, Caixia, Yang, Zuokun, Zerbini, F. Murilo, Zhai, Lifeng, Zhang, Yong-Zhen, Zhang, Song, Zhang, Jinguo, Zhang, Zhe, and Zhou, Xueping

Published
2021
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13. Correction to: 2021 Taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales

Author

Kuhn, Jens H., Adkins, Scott, Agwanda, Bernard R., Al Kubrusli, Rim, Alkhovsky, Sergey V., Amarasinghe, Gaya K., Avšič-Županc, Tatjana, Ayllón, María A., Bahl, Justin, Balkema-Buschmann, Anne, Ballinger, Matthew J., Basler, Christopher F., Bavari, Sina, Beer, Martin, Bejerman, Nicolas, Bennett, Andrew J., Bente, Dennis A., Bergeron, Éric, Bird, Brian H., Blair, Carol D., Blasdell, Kim R., Blystad, Dag-Ragnar, Bojko, Jamie, Borth, Wayne B., Bradfute, Steven, Breyta, Rachel, Briese, Thomas, Brown, Paul A., Brown, Judith K., Buchholz, Ursula J., Buchmeier, Michael J., Bukreyev, Alexander, Burt, Felicity, Büttner, Carmen, Calisher, Charles H., Cao, Mengji, Casas, Inmaculada, Chandran, Kartik, Charrel, Rémi N., Cheng, Qi, Chiaki, Yuya, Chiapello, Marco, Choi, Il-Ryong, Ciuffo, Marina, Clegg, J. Christopher S., Crozier, Ian, Dal Bó, Elena, de la Torre, Juan Carlos, de Lamballerie, Xavier, de Swart, Rik L., Debat, Humberto, Dheilly, Nolwenn M., Di Cicco, Emiliano, Di Paola, Nicholas, Di Serio, Francesco, Dietzgen, Ralf G., Digiaro, Michele, Dolnik, Olga, Drebot, Michael A., Drexler, J. Felix, Dundon, William G., Duprex, W. Paul, Dürrwald, Ralf, Dye, John M., Easton, Andrew J., Ebihara, Hideki, Elbeaino, Toufic, Ergünay, Koray, Ferguson, Hugh W., Fooks, Anthony R., Forgia, Marco, Formenty, Pierre B. H., Fránová, Jana, Freitas-Astúa, Juliana, Fu, Jingjing, Fürl, Stephanie, Gago-Zachert, Selma, Gāo, George Fú, García, María Laura, García-Sastre, Adolfo, Garrison, Aura R., Gaskin, Thomas, Gonzalez, Jean-Paul J., Griffiths, Anthony, Goldberg, Tony L., Groschup, Martin H., Günther, Stephan, Hall, Roy A., Hammond, John, Han, Tong, Hepojoki, Jussi, Hewson, Roger, Hong, Jiang, Hong, Ni, Hongo, Seiji, Horie, Masayuki, Hu, John S., Hu, Tao, Hughes, Holly R., Hüttner, Florian, Hyndman, Timothy H., Ilyas, M., Jalkanen, Risto, Jiāng, Dàohóng, Jonson, Gilda B., Junglen, Sandra, Kadono, Fujio, Kaukinen, Karia H., Kawate, Michael, Klempa, Boris, Klingström, Jonas, Kobinger, Gary, Koloniuk, Igor, Kondō, Hideki, Koonin, Eugene V., Krupovic, Mart, Kubota, Kenji, Kurath, Gael, Laenen, Lies, Lambert, Amy J., Langevin, Stanley L., Lee, Benhur, Lefkowitz, Elliot J., Leroy, Eric M., Li, Shaorong, Li, Longhui, Lǐ, Jiànróng, Liu, Huazhen, Lukashevich, Igor S., Maes, Piet, de Souza, William Marciel, Marklewitz, Marco, Marshall, Sergio H., Marzano, Shin-Yi L., Massart, Sebastien, McCauley, John W., Melzer, Michael, Mielke-Ehret, Nicole, Miller, Kristina M., Ming, Tobi J., Mirazimi, Ali, Mordecai, Gideon J., Mühlbach, Hans-Peter, Mühlberger, Elke, Naidu, Rayapati, Natsuaki, Tomohide, Navarro, José A., Netesov, Sergey V., Neumann, Gabriele, Nowotny, Norbert, Nunes, Márcio R. T., Olmedo-Velarde, Alejandro, Palacios, Gustavo, Pallás, Vicente, Pályi, Bernadett, Papa, Anna, Paraskevopoulou, Sofia, Park, Adam C., Parrish, Colin R., Patterson, David A., Pauvolid-Corrêa, Alex, Pawęska, Janusz T., Payne, Susan, Peracchio, Carlotta, Pérez, Daniel R., Postler, Thomas S., Qi, Liying, Radoshitzky, Sheli R., Resende, Renato O., Reyes, Carina A., Rima, Bertus K., Luna, Gabriel Robles, Romanowski, Víctor, Rota, Paul, Rubbenstroth, Dennis, Rubino, Luisa, Runstadler, Jonathan A., Sabanadzovic, Sead, Sall, Amadou Alpha, Salvato, Maria S., Sang, Rosemary, Sasaya, Takahide, Schulze, Angela D., Schwemmle, Martin, Shi, Mang, Shí, Xiǎohóng, Shí, Zhènglì, Shimomoto, Yoshifumi, Shirako, Yukio, Siddell, Stuart G., Simmonds, Peter, Sironi, Manuela, Smagghe, Guy, Smither, Sophie, Song, Jin-Won, Spann, Kirsten, Spengler, Jessica R., Stenglein, Mark D., Stone, David M., Sugano, Jari, Suttle, Curtis A., Tabata, Amy, Takada, Ayato, Takeuchi, Shigeharu, Tchouassi, David P., Teffer, Amy, Tesh, Robert B., Thornburg, Natalie J., Tomitaka, Yasuhiro, Tomonaga, Keizō, Tordo, Noël, Torto, Baldwyn, Towner, Jonathan S., Tsuda, Shinya, Tu, Changchun, Turina, Massimo, Tzanetakis, Ioannis E., Uchida, Janice, Usugi, Tomio, Vaira, Anna Maria, Vallino, Marta, van den Hoogen, Bernadette, Varsani, Arvind, Vasilakis, Nikos, Verbeek, Martin, von Bargen, Susanne, Wada, Jiro, Wahl, Victoria, Walker, Peter J., Wang, Lin-Fa, Wang, Guoping, Wang, Yanxiang, Wang, Yaqin, Waqas, Muhammad, Wèi, Tàiyún, Wen, Shaohua, Whitfield, Anna E., Williams, John V., Wolf, Yuri I., Wu, Jiangxiang, Xu, Lei, Yanagisawa, Hironobu, Yang, Caixia, Yang, Zuokun, Zerbini, F. Murilo, Zhai, Lifeng, Zhang, Yong-Zhen, Zhang, Song, Zhang, Jinguo, Zhang, Zhe, and Zhou, Xueping

Published
2021
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14. Assessment of Outer Retinal Remodeling in the Hibernating 13-Lined Ground Squirrel

Author

Sajdak, Benjamin S, Bell, Brent A, Lewis, Tylor R, Luna, Gabriel, Cornwell, Grayson S, Fisher, Steven K, Merriman, Dana K, and Carroll, Joseph

Subjects
Biomedical and Clinical Sciences, Ophthalmology and Optometry, Eye Disease and Disorders of Vision, Neurosciences, Biomedical Imaging, Eye, Animals, Basal Metabolism, Cornea, Female, Fluorescent Antibody Technique, Indirect, Hibernation, Male, Microscopy, Electron, Transmission, Retina, Retinal Cone Photoreceptor Cells, Sciuridae, Tomography, Optical Coherence, Torpor, ground squirrel, optical coherence tomography, hibernation, cone photoreceptors, choroid, cornea, Biological Sciences, Medical and Health Sciences, Ophthalmology & Optometry, Ophthalmology and optometry
Abstract

Purpose:We examined outer retinal remodeling of the euthermic and torpid cone-dominant 13-lined ground squirrel (13-LGS) retina using optical coherence tomography (OCT) imaging and histology. Methods:Retinas and corneas of living 13-LGSs were imaged during euthermic and torpid physiological states using OCT. Retinal layer thickness was measured at the visual streak from registered and averaged vertical B-scans. Following OCT, some retinas were collected immediately for postmortem histologic comparison using light microscopy, immunofluorescence, or transmission electron microscopy. Results:Compared to OCT images from euthermic retinae, OCT images of torpid retinae revealed significantly thicker inner and outer nuclear layers, as well as increases in the distances between outer retinal reflectivity bands 1 and 2, and bands 3 and 4. A significant decrease in the distance between bands 2 and 3 also was seen, alongside significant thinning of the choriocapillaris and choroid. OCT image quality was reduced in torpid eyes, partly due to significant thickening of the corneal stroma during this state. Conclusions:The torpid retina of the hibernating 13-LGS undergoes structural changes that can be detected by OCT imaging. Comparisons between in vivo OCT and ex vivo histomorphometry may offer insight to the origin of hyperreflective OCT bands within the outer retina of the cone-dominant 13-LGS.

Published
2018

15. Sox2 regulates astrocytic and vascular development in the retina.

Author

Kautzman, Amanda G, Keeley, Patrick W, Nahmou, Michael M, Luna, Gabriel, Fisher, Steven K, and Reese, Benjamin E

Subjects
Retinal Vessels, Astrocytes, Retina, Animals, Mice, Transgenic, Glial Fibrillary Acidic Protein, SOXB1 Transcription Factors, GFAP, angiogenesis, astrocyte, blood vessels, collagen, vasculature, Eye Disease and Disorders of Vision, Neurodegenerative, Neurosciences, Eye, Neurology & Neurosurgery
Abstract

Sox2 is a transcriptional regulator that is highly expressed in retinal astrocytes, yet its function in these cells has not previously been examined. To understand its role, we conditionally deleted Sox2 from the population of astrocytes and examined the consequences on retinal development. We found that Sox2 deletion does not alter the migration of astrocytes, but it impairs their maturation, evidenced by the delayed upregulation of glial fibrillary acidic protein (GFAP) across the retina. The centro-peripheral gradient of angiogenesis is also delayed in Sox2-CKO retinas. In the mature retina, we observed lasting abnormalities in the astrocytic population evidenced by the sporadic loss of GFAP immunoreactivity in the peripheral retina as well as by the aberrant extension of processes into the inner retina. Blood vessels in the adult retina are also under-developed and show a decrease in the frequency of branch points and in total vessel length. The developmental relationship between maturing astrocytes and angiogenesis suggests a causal relationship between the astrocytic loss of Sox2 and the vascular architecture in maturity. We suggest that the delay in astrocytic maturation and vascular invasion may render the retina hypoxic, thereby causing the abnormalities we observe in adulthood. These studies uncover a novel role for Sox2 in the development of retinal astrocytes and indicate that its removal can lead to lasting changes to retinal homeostasis.

Published
2018

17. Anatomical and Gene Expression Changes in the Retinal Pigmented Epithelium Atrophy 1 (rpea1) Mouse: A Potential Model of Serous Retinal DetachmentCharacterization of the rpea1 Mouse

Author

Luna, Gabriel, Lewis, Geoffrey P, Linberg, Kenneth A, Chang, Bo, Hu, Quiri, Munson, Peter J, Maminishkis, Arvydas, Miller, Sheldon S, and Fisher, Steven K

Subjects
Eye Disease and Disorders of Vision, Neurosciences, 1.1 Normal biological development and functioning, Underpinning research, Eye, Animals, Atrophy, Blotting, Western, DNA, Eye Proteins, Fluorescein Angiography, Fundus Oculi, Gene Expression, Immunohistochemistry, Mice, Mice, Inbred C57BL, Microscopy, Electron, Photoreceptor Cells, Vertebrate, Real-Time Polymerase Chain Reaction, Retinal Detachment, Retinal Pigment Epithelium, Tomography, Optical Coherence, retinal pigment epithelium, cell adhesion, retinal detachment, photoreceptors, extracellular matrix, Biological Sciences, Medical and Health Sciences, Ophthalmology & Optometry
Abstract

PurposeThe purpose of this study was to examine the rpea1 mouse whose retina spontaneously detaches from the underlying RPE as a potential model for studying the cellular effects of serous retinal detachment (SRD).MethodsOptical coherence tomography (OCT) was performed immediately prior to euthanasia; retinal tissue was subsequently prepared for Western blotting, microarray analysis, immunocytochemistry, and light and electron microscopy (LM, EM).ResultsBy postnatal day (P) 30, OCT, LM, and EM revealed the presence of small shallow detachments that increased in number and size over time. By P60 in regions of detachment, there was a dramatic loss of PNA binding around cones in the interphotoreceptor matrix and a concomitant increase in labeling of the outer nuclear layer and rod synaptic terminals. Retinal pigment epithelium wholemounts revealed a patchy loss in immunolabeling for both ezrin and aquaporin 1. Anti-ezrin labeling was lost from small regions of the RPE apical surface underlying detachments at P30. Labeling for tight-junction proteins provided a regular array of profiles outlining the periphery of RPE cells in wild-type tissue, however, this pattern was disrupted in the mutant as early as P30. Microarray analysis revealed a broad range of changes in genes involved in metabolism, signaling, cell polarity, and tight-junction organization.ConclusionsThese data indicate changes in this mutant mouse that may provide clues to the underlying mechanisms of SRD in humans. Importantly, these changes include the production of multiple spontaneous detachments without the presence of a retinal tear or significant degeneration of outer segments, changes in the expression of proteins involved in adhesion and fluid transport, and a disrupted organization of RPE tight junctions that may contribute to the formation of focal detachments.

Published
2016

18. Strategies for bioengineered scaffolds that support adipose stem cells in regenerative therapies

Author

Clevenger, Tracy N, Luna, Gabriel, Fisher, Steven K, and Clegg, Dennis O

Subjects
Medical Biotechnology, Biomedical and Clinical Sciences, Engineering, Biomedical Engineering, Stem Cell Research - Nonembryonic - Non-Human, Stem Cell Research - Induced Pluripotent Stem Cell, Stem Cell Research - Induced Pluripotent Stem Cell - Human, Stem Cell Research, Stem Cell Research - Nonembryonic - Human, Regenerative Medicine, Transplantation, Bioengineering, Development of treatments and therapeutic interventions, 5.2 Cellular and gene therapies, Generic health relevance, Adipose Tissue, Animals, Cell- and Tissue-Based Therapy, Humans, Regeneration, Stem Cell Transplantation, Stem Cells, Tissue Engineering, Tissue Scaffolds, 3D scaffolds, adipose-derived stem cells, ASC, cartilaginous regeneration, osteogenic regeneration, regenerative medicine, soft-tissue regeneration, synthetic scaffold, Developmental Biology, Medical biotechnology, Biomedical engineering
Abstract

Regenerative medicine possesses the potential to ameliorate damage to tissue that results from a vast range of conditions, including traumatic injury, tumor resection and inherited tissue defects. Adult stem cells, while more limited in their potential than pluripotent stem cells, are still capable of differentiating into numerous lineages and provide feasible allogeneic and autologous treatment options for many conditions. Adipose stem cells are one of the most abundant types of stem cell in the adult human. Here, we review recent advances in the development of synthetic scaffolding systems used in concert with adipose stem cells and assess their potential use for clinical applications.

Published
2016

19. Astrocyte structural reactivity and plasticity in models of retinal detachment

Author

Luna, Gabriel, Keeley, Patrick W, Reese, Benjamin E, Linberg, Kenneth A, Lewis, Geoffrey P, and Fisher, Steven K

Subjects
Biomedical and Clinical Sciences, Ophthalmology and Optometry, Neurodegenerative, Eye Disease and Disorders of Vision, Neurosciences, Aging, Aetiology, 2.1 Biological and endogenous factors, Eye, Neurological, Animals, Astrocytes, Cats, Cell Plasticity, Disease Models, Animal, Ependymoglial Cells, Humans, Mice, Mice, Mutant Strains, Rats, Rats, Long-Evans, Retinal Detachment, Retinal Ganglion Cells, Sciuridae, Glia, Astrocyte, Retinal astrocytes, Astrocyte reactivity, Astrocyte hypertrophy astrocyte remodeling, Astrocyte structural plasticity, Retinal detachment, Medical Biochemistry and Metabolomics, Opthalmology and Optometry, Ophthalmology & Optometry, Ophthalmology and optometry
Abstract

Although retinal neurodegenerative conditions such as age-related macular degeneration, glaucoma, diabetic retinopathy, retinitis pigmentosa, and retinal detachment have different etiologies and pathological characteristics, they also have many responses in common at the cellular level, including neural and glial remodeling. Structural changes in Müller cells, the large radial glia of the retina in retinal disease and injury have been well described, that of the retinal astrocytes remains less so. Using modern imaging technology to describe the structural remodeling of retinal astrocytes after retinal detachment is the focus of this paper. We present both a review of critical literature as well as novel work focusing on the responses of astrocytes following rhegmatogenous and serous retinal detachment. The mouse presents a convenient model system in which to study astrocyte reactivity since the Mϋller cell response is muted in comparison to other species thereby allowing better visualization of the astrocytes. We also show data from rat, cat, squirrel, and human retina demonstrating similarities and differences across species. Our data from immunolabeling and dye-filling experiments demonstrate previously undescribed morphological characteristics of normal astrocytes and changes induced by detachment. Astrocytes not only upregulate GFAP, but structurally remodel, becoming increasingly irregular in appearance, and often penetrating deep into neural retina. Understanding these responses, their consequences, and what drives them may prove to be an important component in improving visual outcome in a variety of therapeutic situations. Our data further supports the concept that astrocytes are important players in the retina's overall response to injury and disease.

Published
2016

20. Noninvasive imaging of the thirteen-lined ground squirrel photoreceptor mosaic

Author

SAJDAK, BENJAMIN, SULAI, YUSUFU N, LANGLO, CHRISTOPHER S, LUNA, GABRIEL, FISHER, STEVEN K, MERRIMAN, DANA K, and DUBRA, ALFREDO

Subjects
Biomedical and Clinical Sciences, Ophthalmology and Optometry, Neurosciences, Eye Disease and Disorders of Vision, Animals, Female, Male, Ophthalmoscopy, Retinal Cone Photoreceptor Cells, Retinal Rod Photoreceptor Cells, Sciuridae, Photoreceptors, Adaptive optics, Visual streak, Rodent, Retinal imaging, Medical and Health Sciences, Psychology and Cognitive Sciences, Neurology & Neurosurgery, Ophthalmology and optometry
Abstract

Ground squirrels are an increasingly important model for studying visual processing, retinal circuitry, and cone photoreceptor function. Here, we demonstrate that the photoreceptor mosaic can be longitudinally imaged noninvasively in the 13-lined ground squirrel (Ictidomys tridecemlineatus) using confocal and nonconfocal split-detection adaptive optics scanning ophthalmoscopy using 790 nm light. Photoreceptor density, spacing, and Voronoi analysis are consistent with that of the human cone mosaic. The high imaging success rate and consistent image quality in this study reinforce the ground squirrel as a practical model to aid drug discovery and testing through longitudinal imaging on the cellular scale.

Published
2016

21. Cell-mediated remodeling of biomimetic encapsulating hydrogels triggered by adipogenic differentiation of adipose stem cells.

Author

Clevenger, Tracy N, Luna, Gabriel, Boctor, Daniel, Fisher, Steven K, and Clegg, Dennis O

Subjects
Adipose stem cells, Arg–Gly–Asp, MMP3, biodegradable, biomimetic, poly(ethylene)-glycol hydrogel, regenerative medicine, Arg-Gly-Asp, Transplantation, Bioengineering, Stem Cell Research - Nonembryonic - Non-Human, Stem Cell Research, Biotechnology, Regenerative Medicine, 5.2 Cellular and gene therapies, Biochemistry and Cell Biology, Medical Biotechnology
Abstract

One of the most common regenerative therapies is autologous fat grafting, which frequently suffers from unexpected volume loss. One approach is to deliver adipose stem cells encapsulated in the engineered hydrogels supportive of cell survival, differentiation, and integration after transplant. We describe an encapsulating, biomimetic poly(ethylene)-glycol hydrogel, with embedded peptides for attachment and biodegradation. Poly(ethylene)-glycol hydrogels containing an Arg-Gly-Asp attachment sequence and a matrix metalloprotease 3/10 cleavage site supported adipose stem cell survival and showed remodeling initiated by adipogenic differentiation. Arg-Gly-Asp-matrix metalloprotease 3/10 cleavage site hydrogels showed an increased number and area of lacunae or holes after adipose stem cell differentiation. Image analysis of adipose stem cells in Arg-Gly-Asp-matrix metalloprotease 3/10 cleavage site hydrogels showed larger Voronoi domains, while cell density remained unchanged. The differentiated adipocytes residing within these newly remodeled spaces express proteins and messenger RNAs indicative of adipocytic differentiation. These engineered scaffolds may provide niches for stem cell differentiation and could prove useful in soft tissue regeneration.

Published
2016

23. 2020 taxonomic update for phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales

Author

Kuhn, Jens H., Adkins, Scott, Alioto, Daniela, Alkhovsky, Sergey V., Amarasinghe, Gaya K., Anthony, Simon J., Avšič-Županc, Tatjana, Ayllón, María A., Bahl, Justin, Balkema-Buschmann, Anne, Ballinger, Matthew J., Bartonička, Tomáš, Basler, Christopher, Bavari, Sina, Beer, Martin, Bente, Dennis A., Bergeron, Éric, Bird, Brian H., Blair, Carol, Blasdell, Kim R., Bradfute, Steven B., Breyta, Rachel, Briese, Thomas, Brown, Paul A., Buchholz, Ursula J., Buchmeier, Michael J., Bukreyev, Alexander, Burt, Felicity, Buzkan, Nihal, Calisher, Charles H., Cao, Mengji, Casas, Inmaculada, Chamberlain, John, Chandran, Kartik, Charrel, Rémi N., Chen, Biao, Chiumenti, Michela, Choi, Il-Ryong, Clegg, J. Christopher S., Crozier, Ian, da Graça, John V., Dal Bó, Elena, Dávila, Alberto M. R., de la Torre, Juan Carlos, de Lamballerie, Xavier, de Swart, Rik L., Di Bello, Patrick L., Di Paola, Nicholas, Di Serio, Francesco, Dietzgen, Ralf G., Digiaro, Michele, Dolja, Valerian V., Dolnik, Olga, Drebot, Michael A., Drexler, Jan Felix, Dürrwald, Ralf, Dufkova, Lucie, Dundon, William G., Duprex, W. Paul, Dye, John M., Easton, Andrew J., Ebihara, Hideki, Elbeaino, Toufic, Ergünay, Koray, Fernandes, Jorlan, Fooks, Anthony R., Formenty, Pierre B. H., Forth, Leonie F., Fouchier, Ron A. M., Freitas-Astúa, Juliana, Gago-Zachert, Selma, Gāo, George Fú, García, María Laura, García-Sastre, Adolfo, Garrison, Aura R., Gbakima, Aiah, Goldstein, Tracey, Gonzalez, Jean-Paul J., Griffiths, Anthony, Groschup, Martin H., Günther, Stephan, Guterres, Alexandro, Hall, Roy A., Hammond, John, Hassan, Mohamed, Hepojoki, Jussi, Hepojoki, Satu, Hetzel, Udo, Hewson, Roger, Hoffmann, Bernd, Hongo, Seiji, Höper, Dirk, Horie, Masayuki, Hughes, Holly R., Hyndman, Timothy H., Jambai, Amara, Jardim, Rodrigo, Jiāng, Dàohóng, Jin, Qi, Jonson, Gilda B., Junglen, Sandra, Karadağ, Serpil, Keller, Karen E., Klempa, Boris, Klingström, Jonas, Kobinger, Gary, Kondō, Hideki, Koonin, Eugene V., Krupovic, Mart, Kurath, Gael, Kuzmin, Ivan V., Laenen, Lies, Lamb, Robert A., Lambert, Amy J., Langevin, Stanley L., Lee, Benhur, Lemos, Elba R. S., Leroy, Eric M., Li, Dexin, Lǐ, Jiànróng, Liang, Mifang, Liú, Wénwén, Liú, Yàn, Lukashevich, Igor S., Maes, Piet, Marciel de Souza, William, Marklewitz, Marco, Marshall, Sergio H., Martelli, Giovanni P., Martin, Robert R., Marzano, Shin-Yi L., Massart, Sébastien, McCauley, John W., Mielke-Ehret, Nicole, Minafra, Angelantonio, Minutolo, Maria, Mirazimi, Ali, Mühlbach, Hans-Peter, Mühlberger, Elke, Naidu, Rayapati, Natsuaki, Tomohide, Navarro, Beatriz, Navarro, José A., Netesov, Sergey V., Neumann, Gabriele, Nowotny, Norbert, Nunes, Márcio R. T., Nylund, Are, Økland, Arnfinn L., Oliveira, Renata C., Palacios, Gustavo, Pallas, Vicente, Pályi, Bernadett, Papa, Anna, Parrish, Colin R., Pauvolid-Corrêa, Alex, Pawęska, Janusz T., Payne, Susan, Pérez, Daniel R., Pfaff, Florian, Radoshitzky, Sheli R., Rahman, Aziz-ul, Ramos-González, Pedro L., Resende, Renato O., Reyes, Carina A., Rima, Bertus K., Romanowski, Víctor, Robles Luna, Gabriel, Rota, Paul, Rubbenstroth, Dennis, Runstadler, Jonathan A., Ruzek, Daniel, Sabanadzovic, Sead, Salát, Jiří, Sall, Amadou Alpha, Salvato, Maria S., Sarpkaya, Kamil, Sasaya, Takahide, Schwemmle, Martin, Shabbir, Muhammad Z., Shí, Xiǎohóng, Shí, Zhènglì, Shirako, Yukio, Simmonds, Peter, Širmarová, Jana, Sironi, Manuela, Smither, Sophie, Smura, Teemu, Song, Jin-Won, Spann, Kirsten M., Spengler, Jessica R., Stenglein, Mark D., Stone, David M., Straková, Petra, Takada, Ayato, Tesh, Robert B., Thornburg, Natalie J., Tomonaga, Keizō, Tordo, Noël, Towner, Jonathan S., Turina, Massimo, Tzanetakis, Ioannis, Ulrich, Rainer G., Vaira, Anna Maria, van den Hoogen, Bernadette, Varsani, Arvind, Vasilakis, Nikos, Verbeek, Martin, Wahl, Victoria, Walker, Peter J., Wang, Hui, Wang, Jianwei, Wang, Xifeng, Wang, Lin-Fa, Wèi, Tàiyún, Wells, Heather, Whitfield, Anna E., Williams, John V., Wolf, Yuri I., Wú, Zhìqiáng, Yang, Xin, Yáng, Xīnglóu, Yu, Xuejie, Yutin, Natalya, Zerbini, F. Murilo, Zhang, Tong, Zhang, Yong-Zhen, Zhou, Guohui, and Zhou, Xueping

Published
2020
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24. Three-dimensional organization of nascent rod outer segment disk membranes

Author

Volland, Stefanie, Hughes, Louise C, Kong, Christina, Burgess, Barry L, Linberg, Kenneth A, Luna, Gabriel, Zhou, Z Hong, Fisher, Steven K, and Williams, David S

Subjects
Earth Sciences, Biological Sciences, Geology, Eye Disease and Disorders of Vision, Underpinning research, 1.1 Normal biological development and functioning, Animals, Cats, Cilia, Intracellular Membranes, Macaca mulatta, Mice, Retinal Rod Photoreceptor Cells, photoreceptor, cilium, EM tomography, disk morphogenesis
Abstract

The vertebrate photoreceptor cell contains an elaborate cilium that includes a stack of phototransductive membrane disks. The disk membranes are continually renewed, but how new disks are formed remains poorly understood. Here we used electron microscope tomography to obtain 3D visualization of the nascent disks of rod photoreceptors in three mammalian species, to gain insight into the process of disk morphogenesis. We observed that nascent disks are invariably continuous with the ciliary plasma membrane, although, owing to partial enclosure, they can appear to be internal in 2D profiles. Tomographic analyses of the basal-most region of the outer segment show changes in shape of the ciliary plasma membrane indicating an invagination, which is likely a first step in disk formation. The invagination flattens to create the proximal surface of an evaginating lamella, as well as membrane protrusions that extend between adjacent lamellae, thereby initiating a disk rim. Immediately distal to this initiation site, lamellae of increasing diameter are evident, indicating growth outward from the cilium. In agreement with a previous model, our data indicate that mature disks are formed once lamellae reach full diameter, and the growth of a rim encloses the space between adjacent surfaces of two lamellae. This study provides 3D data of nascent and mature rod photoreceptor disk membranes at unprecedented z-axis depth and resolution, and provides a basis for addressing fundamental questions, ranging from protein sorting in the photoreceptor cilium to photoreceptor electrophysiology.

Published
2015

26. Characterizing spatial distributions of astrocytes in the mammalian retina

Author

Jammalamadaka, Aruna, Suwannatat, Panuakdet, Fisher, Steven K, Manjunath, BS, Höllerer, Tobias, and Luna, Gabriel

Subjects
Neurosciences, Eye Disease and Disorders of Vision, Animals, Astrocytes, Cells, Cultured, Image Processing, Computer-Assisted, Mice, Neurons, Retina, Mathematical Sciences, Biological Sciences, Information and Computing Sciences, Bioinformatics
Abstract

MotivationIn addition to being involved in retinal vascular growth, astrocytes play an important role in diseases and injuries, such as glaucomatous neuro-degeneration and retinal detachment. Studying astrocytes, their morphological cell characteristics and their spatial relationships to the surrounding vasculature in the retina may elucidate their role in these conditions.ResultsOur results show that in normal healthy retinas, the distribution of observed astrocyte cells does not follow a uniform distribution. The cells are significantly more densely packed around the blood vessels than a uniform distribution would predict. We also show that compared with the distribution of all cells, large cells are more dense in the vicinity of veins and toward the optic nerve head whereas smaller cells are often more dense in the vicinity of arteries. We hypothesize that since veinal astrocytes are known to transport toxic metabolic waste away from neurons they may be more critical than arterial astrocytes and therefore require larger cell bodies to process waste more efficiently.Availability and implementationA 1/8th size down-sampled version of the seven retinal image mosaics described in this article can be found on BISQUE (Kvilekval et al., 2010) at http://bisque.ece.ucsb.edu/client_service/view?resource=http://bisque.ece.ucsb.edu/data_service/dataset/6566968.

Published
2015

27. Viral-mediated RdCVF and RdCVFL expression protects cone and rod photoreceptors in retinal degeneration

Author

Byrne, Leah C, Dalkara, Deniz, Luna, Gabriel, Fisher, Steven K, Clérin, Emmanuelle, Sahel, Jose-Alain, Léveillard, Thierry, and Flannery, John G

Subjects
Gene Therapy, Genetics, Neurosciences, Eye Disease and Disorders of Vision, 5.2 Cellular and gene therapies, Development of treatments and therapeutic interventions, Eye, Animals, Cell Survival, Dependovirus, Evoked Potentials, Visual, Eye Proteins, Gene Expression, Gene Transfer Techniques, Genetic Therapy, Mice, Inbred C57BL, Photic Stimulation, Retinal Cone Photoreceptor Cells, Retinal Degeneration, Retinal Rod Photoreceptor Cells, Rhodopsin, Thioredoxins, Transduction, Genetic, Medical and Health Sciences, Immunology
Abstract

Alternative splicing of nucleoredoxin-like 1 (Nxnl1) results in 2 isoforms of the rod-derived cone viability factor. The truncated form (RdCVF) is a thioredoxin-like protein secreted by rods that promotes cone survival, while the full-length isoform (RdCVFL), which contains a thioredoxin fold, is involved in oxidative signaling and protection against hyperoxia. Here, we evaluated the effects of these different isoforms in 2 murine models of rod-cone dystrophy. We used adeno-associated virus (AAV) to express these isoforms in mice and found that both systemic and intravitreal injection of engineered AAV vectors resulted in RdCVF and RdCVFL expression in the eye. Systemic delivery of AAV92YF vectors in neonates resulted in earlier onset of RdCVF and RdCVFL expression compared with that observed with intraocular injection using the same vectors at P14. We also evaluated the efficacy of intravitreal injection using a recently developed photoreceptor-transducing AAV variant (7m8) at P14. Systemic administration of AAV92YF-RdCVF improved cone function and delayed cone loss, while AAV92YF-RdCVFL increased rhodopsin mRNA and reduced oxidative stress by-products. Intravitreal 7m8-RdCVF slowed the rate of cone cell death and increased the amplitude of the photopic electroretinogram. Together, these results indicate different functions for Nxnl1 isoforms in the retina and suggest that RdCVF gene therapy has potential for treating retinal degenerative disease.

Published
2015

28. Development and Plasticity of Outer Retinal Circuitry Following Genetic Removal of Horizontal Cells

Author

Keeley, Patrick W, Luna, Gabriel, Fariss, Robert N, Skyles, Kimberly A, Madsen, Nils R, Raven, Mary A, Poché, Ross A, Swindell, Eric C, Jamrich, Milan, Oh, Edwin C, Swaroop, Anand, Fisher, Steven K, and Reese, Benjamin E

Subjects
Neurosciences, Pediatric, Eye Disease and Disorders of Vision, Animals, Female, Mice, Mice, Transgenic, Neuronal Plasticity, Retinal Horizontal Cells, Retinal Neurons, Synapses, Visual Pathways, Medical and Health Sciences, Psychology and Cognitive Sciences, Neurology & Neurosurgery
Abstract

The present study examined the consequences of eliminating horizontal cells from the outer retina during embryogenesis upon the organization and assembly of the outer plexiform layer (OPL). Retinal horizontal cells exhibit a migration defect in Lim1-conditional knock-out (Lim1-CKO) mice and become mispositioned in the inner retina before birth, redirecting their dendrites into the inner plexiform layer. The resultant (mature) OPL, developing in the absence of horizontal cells, shows a retraction of rod spherules into the outer nuclear layer and a sprouting of rod bipolar cell dendrites to reach ectopic ribbon-protein puncta. Cone pedicles and the dendrites of type 7 cone bipolar cells retain their characteristic stratification and colocalization within the collapsed OPL, although both are atrophic and the spatial distribution of the pedicles is disrupted. Developmental analysis of Lim1-CKO retina reveals that components of the rod and cone pathways initially co-assemble within their normal strata in the OPL, indicating that horizontal cells are not required for the correct targeting of photoreceptor terminals or bipolar cell dendrites. As the rod spherules begin to retract during the second postnatal week, rod bipolar cells initially show no signs of ectopic growth, sprouting only subsequently and continuing to do so well after the eighth postnatal week. These results demonstrate the critical yet distinctive roles for horizontal cells on the rod and cone pathways and highlight a unique and as-yet-unrecognized maintenance function of an inhibitory interneuron that is not required for the initial targeting and co-stratification of other components in the circuit.

Published
2013

29. Optomotor and immunohistochemical changes in the juvenile S334ter rat

Author

McGill, Trevor J, Prusky, Glen T, Luna, Gabriel, LaVail, Matthew M, Fisher, Steven K, and Lewis, Geoffrey P

Subjects
Biomedical and Clinical Sciences, Ophthalmology and Optometry, Eye Disease and Disorders of Vision, Neurosciences, Eye, Animals, Biomarkers, Cell Survival, Electroretinography, Fluorescent Antibody Technique, Indirect, Microscopy, Confocal, Mutation, Neuroglia, Nystagmus, Optokinetic, Photoreceptor Cells, Vertebrate, Rats, Rats, Long-Evans, Rats, Transgenic, Retinal Degeneration, Retinal Ganglion Cells, Rhodopsin, Sensory Thresholds, Visual Perception, rhodopsin, optokinetic tracking, transgenic, retinal degeneration, retinal remodeling, Medical Biochemistry and Metabolomics, Opthalmology and Optometry, Ophthalmology & Optometry, Ophthalmology and optometry
Abstract

The aim of this study was to examine the temporal relationship between behaviorally measured visual thresholds, photoreceptor degeneration and dysfunction, synaptic and neuronal morphology changes in the retina in the S334ter line 4 rat. Specifically, we examined the optokinetic tracking (OKT) behavior in S334ter rats daily and found that OKT thresholds reflected normal values at eye opening but quickly reduced to a non-response level by postnatal day (P) 22. By contrast, the scotopic electroretinogram (ERG) showed a much slower degeneration, with substantial scotopic function remaining after P90 as previously demonstrated for this line of rats. Photopic b-wave amplitudes revealed functional levels between 70 and 100% of normal between P30 and P90. Histological evidence demonstrated that photoreceptor degeneration occurred over many months, with an outer nuclear layer (ONL) roughly half the thickness of a normal age-matched control at P90. Immunohistochemical analysis revealed a number of changes in retinal morphology in the Tg S334ter line 4 rat that occur at or before P40 including: elevated levels of rod opsin expression in the ONL, cone photoreceptor morphology changes, glial cell activation, inner retinal neuron sprouting, and microglial cell activation. Many of these changes were evident at P30 and in some cases as early as eye opening (P15). Thus, the morphological changes occurred in concert with or before the very rapid loss of the behavioral (OKT) responses, and significantly before the loss of photoreceptors and photoreceptor function.

Published
2012

35. Literacy program to improve the education of the Agta

Author

Luna, Gabriel and Luna, Gabriel

Abstract

The study sought to identify the cultural profile, beliefs and practices, attitude toward literacy education, and Agta problems and aspirations as an input for the Agta literacy program. Descriptive The profile variables were determined through research as an input to the literacy program to improve Agta's education. According to the findings, the Agta got their name from prominent people, their dialect is Upos, and they live in a simple and temporary shelter. Furthermore, the male Agta served as the family's head and provider, while the female Agta assisted with child rearing and housekeeping. They prefer to eat foods found in their environment and spend their leisure time bonding with their fellow Agta and chewing betel nuts, momma, or nganga. The Agta, on the other hand, have a rich cultural heritage regarding the stages of life such as conception, childbirth, child rearing, courtship, marriage, and death and burial. The majority of Agta are illiterate. They demonstrated a positive and extremely favorable attitude toward literacy education. Agta has concerns and desires, particularly regarding their children's education. It was also discovered that stakeholders and the Local Government Unit support Agta education, so implementing a literacy program for Agta to improve their education is highly recommended.

Published
2023

36. Carta de Psicología No. 57

Author

López-Cantero, Ever José, Perdomo-Escobar, Sandra Jimena, Ayala-Góngora, Angie Paola, Torres-Marques, Samar Elizabeth, Córdoba-Martínez, María Camila, Romero-Cortés, Íngrid, Pulido-Escobar, Paola Andrea, Bolívar-Rodríguez, Laura Daniela, Álvarez-Herrera, Ingrid Valentina, Torres-Campos, María Camila, Prieto-Arias Nicole Daniela, Camargo-Escobar, Ítala Marina, Velásquez Sarasty, Lisandro, Ramírez-Velandia, Andrés Fernando, Aranguren-Fonseca, Laura Daniela, Gómez-Moreno, Yulieth Fernanda, Tello-Jiménez, Ana María, Sanmartín-Galvis, María Camila, Zuleta-Munive, María José, Pinto-Parra, Valentina, Silva-Bohórquez, Isabela, Avendaño-Montañez, Andrés Felipe, Camargo-Rojas, Karol Andrea, Cruz-Gutiérrez, Maribel, Díaz-Acevedo, Valentina, Rueda-González, María Camila, Zuluaga-Canay, Jessica Katherine, Bernal-Castro, Karen Daniela, González, María Fernanda, Pardo-Calderón, Amaranta, Ayala-Rodríguez, Nelly, Blanco-Rivero, Fabián, Garzón-Quiroga, Camila, Puro-Landivar, Rafael, Casas-Soriano, David Santiago, Saavedra-Chávez, Juan Felipe, Cortés-de Narváez, Laura Geraldine, Pinzón-Serrano, Manuela María del Rocío, Almeida-Luna, Gabriel Eduardo, Alejo-Castañeda, Ivonne Edith, Cortés-Ospina, Daiani Neriad, Díaz-Rico, Sara, Santacoloma-Suárez, Andrés Mauricio, D’Achiardi-Gómez, Luisa Fernanda, Arévalo-Paiva, Carol Dahana, Lugo-Wilches, Karla Sofía, Wilches-Escobar, María Paula, González-Alarcón, Xiomara, Gómez-Blanco, Johanna, Moreno-López, Estefani, Larreal-Morales, Santiago, Pulido, Paola, López-Cantero, Ever José, Perdomo-Escobar, Sandra Jimena, Ayala-Góngora, Angie Paola, Torres-Marques, Samar Elizabeth, Córdoba-Martínez, María Camila, Romero-Cortés, Íngrid, Pulido-Escobar, Paola Andrea, Bolívar-Rodríguez, Laura Daniela, Álvarez-Herrera, Ingrid Valentina, Torres-Campos, María Camila, Prieto-Arias Nicole Daniela, Camargo-Escobar, Ítala Marina, Velásquez Sarasty, Lisandro, Ramírez-Velandia, Andrés Fernando, Aranguren-Fonseca, Laura Daniela, Gómez-Moreno, Yulieth Fernanda, Tello-Jiménez, Ana María, Sanmartín-Galvis, María Camila, Zuleta-Munive, María José, Pinto-Parra, Valentina, Silva-Bohórquez, Isabela, Avendaño-Montañez, Andrés Felipe, Camargo-Rojas, Karol Andrea, Cruz-Gutiérrez, Maribel, Díaz-Acevedo, Valentina, Rueda-González, María Camila, Zuluaga-Canay, Jessica Katherine, Bernal-Castro, Karen Daniela, González, María Fernanda, Pardo-Calderón, Amaranta, Ayala-Rodríguez, Nelly, Blanco-Rivero, Fabián, Garzón-Quiroga, Camila, Puro-Landivar, Rafael, Casas-Soriano, David Santiago, Saavedra-Chávez, Juan Felipe, Cortés-de Narváez, Laura Geraldine, Pinzón-Serrano, Manuela María del Rocío, Almeida-Luna, Gabriel Eduardo, Alejo-Castañeda, Ivonne Edith, Cortés-Ospina, Daiani Neriad, Díaz-Rico, Sara, Santacoloma-Suárez, Andrés Mauricio, D’Achiardi-Gómez, Luisa Fernanda, Arévalo-Paiva, Carol Dahana, Lugo-Wilches, Karla Sofía, Wilches-Escobar, María Paula, González-Alarcón, Xiomara, Gómez-Blanco, Johanna, Moreno-López, Estefani, Larreal-Morales, Santiago, and Pulido, Paola

Published
2023

37. Literacy Program to Improve the Education of the Agta

Author

Luna, Gabriel L.

Subjects
Attitude Towards Literacy Education, Beliefs and Practices, Cultural Profile, Agta, Problems and Aspirations
Abstract

The study sought to identify the cultural profile, beliefs and practices, attitude toward literacy education, and Agta problems and aspirations as an input for the Agta literacy program. Descriptive The profile variables were determined through research as an input to the literacy program to improve Agta's education. According to the findings, the Agta got their name from prominent people, their dialect is Upos, and they live in a simple and temporary shelter. Furthermore, the male Agta served as the family's head and provider, while the female Agta assisted with child rearing and housekeeping. They prefer to eat foods found in their environment and spend their leisure time bonding with their fellow Agta and chewing betel nuts, momma, or nganga. The Agta, on the other hand, have a rich cultural heritage regarding the stages of life such as conception, childbirth, child rearing, courtship, marriage, and death and burial. The majority of Agta are illiterate. They demonstrated a positive and extremely favorable attitude toward literacy education. Agta has concerns and desires, particularly regarding their children's education. It was also discovered that stakeholders and the Local Government Unit support Agta education, so implementing a literacy program for Agta to improve their education is highly recommended.

Published
2023

38. XIII Jornada de Investigación 2022

Author

Martín-Fiorino, Víctor Rafael, Guadarrama-González, Pablo Manuel, Cortés-Ospina, Daiani Neriad, Díaz-Rico, Sara, Santacoloma-Suárez, Andrés Mauricio, Jiménez-Valderrama, Fernando Augusto, Quintero-Mora, Jasbleydy Victoria, Palacios-Ramos, Magda Alejandra, López-Ruiz, Paola Andrea, Rubiano-Cortés, Melissa María, Gutiérrez-Melo, Michel Dayana, González-Naranjo, Juan David, Aguilar-Pardo, David Ricardo, Muñoz-Obregón, María Alejandra, Romero-Aguilera, David Esteban, Nonsoque-Ruiz, Santiago José, González-Rojas, Camilo, Suárez-Sánchez, Nicole Nahom, Bonilla-Ávila, Dayanna Carolina, Rico-Melo, Gishet Mariana, Zúñiga-Barros, Iván Antonio, Tole-Acosta, Henry David, Acosta-Cipagauta, Christian Johnatan, Vallejo-Cagueñas, Jharol Orlando, Martínez-Pardo, Daniel Stiven, García-Paiba, Deyvi Felipe, Pérez-Riveros, José Miguel, Caicedo-Medina, Andrés Felipe, Solórzano, Indira Daniela, Corredor-Patiño, Laura Valentina, Pinilla-Triana, Luna Estefany, Gómez-Duarte, Javier Felipe, Arévalo-Muñetón, María Paula, Bastidas-Suárez, Douglas Mauricio, Ramírez-León, Lina Marcela, Barrios-Alfonso, Miguel Ángel, Romero-Babativa, Laura Valentina, Martínez-Acosta, Karen Sofía, Salgado-Cadavis, Gonzalo, Adoryan-Machado, María Luiza, Orjuela-Hernández, Andrea, Sánchez, Marco, Medina-Carrión, Ángela María, Chaves-Wilches, Laura Daniela, Jiménez-Barrera, Lina Vanessa, Sierra-Zamora, Paola Alexandra, Gil-Millán, Diego Alejandro, Cortés-López, Yerson Fahir, Carmona-Donoso, Erika Johanna, Guerrero-Gutiérrez, Paola Andrea, Coba-León, Camilo Andrés, Álvarez-Ortiz, Héctor Fernando, Mateus-Poveda, Ana María, Nicolaysen-Sánchez, Hans, Cely-Carvajal, Valentina, Cubides-Cárdenas, Jaime, Tequia-Fonseca, Andrea Marcela, Malagón-Riaño, Angie Paola, Muñoz-Molina, Tatiana, Vergara-Baquero, May Steven, Santana-Sua, Andrea Lizeth, Garavito-García, Luisa Fernanda, Almeida-Luna, Gabriel Eduardo, Alejo-Castañeda, Ivonne Edith, Manrique-Zuluaga, Tatiana, Rodríguez-Bautista, Ana María, Ruiz, Laura Valentina, Bernal, María Paula, Arévalo, Daniela, Moreno-Garzón, Diana Catalina, Moreno-Ardila, Karen Vanessa, Londoño-Fernández, María Camila, Vivas-Becerra, Camilo Jesús Alejandro, Mejía-Parrado, Paula Stefani, Jiménez-Carranza, Claudia Constanza, Aguilar-Bustamante, María Constanza, García-Bernal de Moncada, Doris, Gómez-Pedraza, Mauricio Alexander, Meneses-Báez, Alba Lucía, Sierra-Martínez, Marge Alejandra, Ortiz-Garzón, Eliana Yvette, Da Silva-Carvalho, Marcelo, Basto-Urbina, Estefanny Pamela, Alarcón-Rodríguez, Oscar Mauricio, Ramírez-Cuastuza, Anna Gabriela, Casas-Matiz, Elvia Isabel, Reyes-González, Alberto, García-Delgado, Nicolás, Acosta-Peña, María Camila, Martínez-Parra, Miguel Ángel, Pulido-Escobar, Paola Andrea, Rey-Gómez, Nathalia Patricia, Rodríguez-Prieto, Astrid Carolina, Santamaría-Uribe, Laura Sofía, Carmona-Rodríguez, Nicolás Fernando, Redondo, Johan Manuel, Yunis-Gómez, María Alejandra, Abello-Ávila, Nataly, Daza-Fonseca, Mateo, Martínez-Ochoa, Andrés David, Rodríguez-Romero, Pablo Enrique, Pardo-Galvis, Diana Paola, Algarra-Martínez, Erika Natalia, Ramírez-Velandia, Andrés Fernando, Sanmartín-Galvis, María Camila, Cruz-Gutiérrez, Maribel, Gutiérrez-Gaona, Yuliana, Díaz-Acevedo, Valentina, Camargo-Rojas, Karol Andrea, Villalba-Novoa, María Paula, Zuleta-Munive, María José, Bernal-Castro, Karen Daniela, Zuluaga-Canay, Jessica Katherine, González-Ceballos, María Fernanda, Varón-Melo, Karen Gisell, López-Coy, Juan Pablo, Ospina-Ortiz, Mariana, Albarracín-Mariño, María Camila, Barrios-Rodríguez, Cristian Bernardo, Meneses-Herrera, Camilo Andrés, Franco-Burgos, Jorge Ricardo, Ramírez-Salazar, Yesica Liliana, Valero-Melo, Lizeth Natalia, Cagua-Marroquín, Juan Sebastián, Baquero-Maldonado, Viviana Alejandra, Herrera-Cano, Camilo Ernesto, Riveros-Franco, Juan Camilo, Pulido-Bermeo, María Camila, Gómez-Rivera, David Felipe, Slebi-Acevedo, Carlos José, Rojas-Bedoya, Daniela, Yépez-Arrieta, Álvaro Javier, Bolívar-Barón, Holman Diego, Mejía-Morales, Juan David, Carrillo-Barón, Joan Sebastián, Contreras-Cifuentes, Angie Tatiana, Ríos-Cruz, Sonia Gisela, Caicedo-Támara, Natalia, Domínguez-Rozo, Andrés David, Barrero-Calixto, Juan Carlos, Beltrán-Rojas, Maikol Javie, Montero-Hernández, César Augusto, Castañeda-Tarquino, Daniel Francisco, Chacón-Ruiz, Jhon Erick, Tabares-Chaparro, Bryan Arley, Jara-Pardo, Diana Paola, Linares-Contento, Angie Katherine, Fajardo-Prada, David Mauricio, Pardo-Angulo, Fredy Ernesto, Argüello-Pintor, María Daniela, Nieto-Barbosa, Valentina, Tibério-Cardoso, Grace, Martínez-Rodríguez, Madeline Sofía, Carvajal-Builes, Juan Camilo, García-Ramírez, Laura Daniela, and Barrios-Rodríguez, Cristian Bernardo

Subjects
José Martí, MEDICAMENTOS PATENTADOS, NIÑOS Y GUERRA, DELITOS TRANSNACIONALES, Comunidad, MATERNIDAD SUSTITUTA, VIOLENCIA POLÍTICA-COLOMBIA, PSICÓLOGOS-FORMACIÓN PROFESIONAL, EUTANASIA – LEGISLACIÓN-COLOMBIA, PUEBLOS INDÍGENAS-REUBICACIÓN, ASILOS DE ANCIANOS-DISEÑO SIN BARRERAS, LIBERTAD - ASPECTOS POLÍTICOS, SEGURIDAD SOCIAL – LEGISLACIÓN-COLOMBIA, HOSPITALIDAD, TESTIMONIO (TEORÍA DEL CONOCIMIENTO), ASISTENCIA A LA VEJEZ, IMAGEN CORPORAL, NEUROPSICOLOGÍA PEDIÁTRICA, ALTRUISMO, VIDEOJUEGOS EN LA EDUCACIÓN, TURISMO SEXUAL-COLOMBIA, DELITOS CONTRA DEFENSORES DE DERECHOS HUMANOS
Abstract

Los desafíos en las dinámicas económicas, sociales, políticas y psicológicas han puesto de relieve la importancia de involucrar en la enseñanza universitaria actividades que conecten a los estudiantes con las realidades del contexto en el que se desarrollan; simultáneamente, hoy más que nunca se hace evidente que la ciencia, la tecnología y la innovación (CTI) son cruciales para atender los retos sociales, ambientales y económicos de las sociedades actuales. En este contexto, la Jornada de Investigación de la Universidad Católica de Colombia es quizás uno de los espacios institucionales más emblemáticos en el que se visibilizan las actividades en CTI de estudiantes, jóvenes investigadores y profesores, que buscan contribuir a la solución de problemáticas relevantes del entorno. En esta oportunidad, aproximadamente 177 autores y más de 250 espectadores se dieron cita en un escenario virtual que permitió el intercambio de saberes y conocimientos en torno a muchos temas con un lenguaje común: el bienestar de la humanidad y la respuesta efectiva a los retos que tenemos como comunidad. Esta cuarta versión de las Memorias compila las ponencias presentadas en la XIII Jornada de Investigación de 2022, enmarcadas en los tres ejes temáticos de investigación que tiene la Universidad Católica de Colombia: i) Derecho, Cultura y Sociedad, ii) Desarrollo Humano y Sostenible, y iii) Gestión de la Tecnología al Servicio de la Sociedad. Desde la Dirección Central de Investigaciones nos encontramos profundamente agradecidos con todos y cada uno de los participantes, y nos sentimos aún más orgullosos por la calidad de los trabajos presentados. Sea esta la oportunidad para hacer extensiva una felicitación a los autores y a las diferentes Unidades Académicas que, con su compromiso e invaluable labor, permitieron que este evento se desarrollara con éxito. Tercera edición Persona, hospitalidad y construcción de comunidad desde la fraternidad. José Martí: acerca de la libertad en la condición humana. Moda, imagen y alimentación: una tríada para el bien y para el mal. Lecciones de la pandemia de covid-19: conflictos entre la protección jurídica de las patentes farmacéuticas y el interés general de la salud pública. Psicología y sexualidad: propuesta para la formación de psicólogos colombianos. Estrategias para el desarrollo de herramientas que fomentan el aprendizaje para el reconocimiento y la apropiación del patrimonio cultural. Análisis descriptivo de relatos honestos y deshonestos por medio del sistema de evaluación global. El uso de la herramienta LIWC para el estudio de relatos altruistas y prosociales. ¿De qué manera influyen las redes sociales como medio de información en campañas políticas?. Sistema de Seguridad Social en Colombia: una crisis deficitaria que se refuerza con el tiempo. Turismo sexual en menores de edad: problemática endémica en el territorio colombiano. Transgresión de los derechos humanos en relación con los asesinatos de líderes sociales en el Estado colombiano. El nuevo escenario para la procedencia de la eutanasia en Colombia: una mirada desde los derechos humanos. Protección de los derechos de la infancia frente a los grupos al margen de la ley. El derecho a un nivel de vida adecuado: un enfoque hacia el desplazamiento forzado de los pueblos indígenas. Tráfico de órganos humanos: delito transnacional que vulnera los derechos humanos y su regulación en el marco jurídico colombiano. Transgresión de los derechos a la vida y la libertad en el sistema penitenciario como consecuencia de la violencia social. Acceso a la justicia colombiana en tiempos de SARS-CoV-2. Prioridades para la administración de justicia penal en Colombia: ¿balanceando espectáculo e indicadores de eficacia? Desarrollo de competencias para la investigación en neuropsicología: experiencia del semillero experimental. La dificultad al ingreso de los centros geriátricos. Principio de realidad sobre la formalidad constitucional en contrato verbal laboral en un satélite del Consultorio Jurídico de la Universidad Católica de Colombia. La pena de muerte en colisión con los derechos fundamentales. Maternidad subrogada: objetificación y vulneración de los derechos de la mujer. Una dieta inconsciente hacia el vegetarianismo. Sistema de evaluación del bienestar gerontológico para un diseño arquitectónico sostenible. Caso de estudio: hogar de paso San Francisco de Asís, Villavicencio, Meta. Revisión sistemática interacción líder-colaborador: futuras investigaciones. Análisis conceptual del talento académico desde los modelos teóricos que lo sustentan. Identificación de potenciales factores de riesgo suicida: una mirada contextual. La regulación emocional en tiempos de coronavirus. GEES: Guía de Evaluación de Edificaciones Sostenibles, vivienda de interés social, clima cálido húmedo. Estructura proyectual y sostenible para el diseño y desarrollo de un modelo de vivienda de madera en San Andrés y Providencia, Colombia. Innovación social para la gestión territorial. Construcción de material didáctico para el entrenamiento en habilidades de regulación emocional e interpersonales dirigidas a poblaciones expuestas a situaciones de violencia política. Alternativas sostenibles de modelos de desarrollo industrial. La ruralidad dentro de los procesos del desarrollo local en Usme. Se ha dejado de dibujar arquitectura con las manos. Calidad de vida, bienestar y felicidad en el trabajo: una revisión sistemática de la literatura científica, 2011-2021. Autonomía, autorregulación y educación moral: reflexiones desde la psicología del desarrollo moral. Características de los niños, niñas y adolescentes expuestos a contextos de conflicto armado en Colombia. Revisión bibliométrica de artículos sobre la crianza en niños, niñas y adolescentes colombianos. Narrativas sociales en el proceso de cualificación de lo público La habitación exterior como extensión de la vivienda. Utilización de nanopartículas magnéticas Fe3O4 y ozono para la degradación/eliminación de azul de metileno en agua residual textil. Estudio paramétrico de un modelo numérico Fem de un ensayo CBR. ¿Cómo construir identidad de manera incluyente a partir del reconocimiento de patrimonio cultural construido?. Veracidad de los resultados del ensayo de penetración dinámica de CONO(PDC). Análisis de texto a partir del procesamiento de lenguaje natural para identificar sintomatología depresiva en redes sociales. Análisis de texto para la detección de depresión en comentarios de usuarios de la red social Instagram. Diseño y desarrollo de un videojuego para evaluar la interacción de las redes atencionales en la sintomatología depresiva. Herramienta tecnológica para el apoyo en la detección de sintomatología ansiosa en jóvenes. Optimización del despliegue de aplicaciones web a partir de computación en nube sin servidor. Prototipo alfa de un videojuego serio para el apoyo en la detección de sintomatología depresiva en adultos jóvenes. La importancia de la visualización de datos en la era del Big Data y sus herramientas. Prototipo de sensor para el registro electroencefalográfico. Prototipo de un algoritmo basado en inteligencia artificial para el apoyo a especialistas en el diagnóstico del Alzheimer. Evaluación posocupacional del confort térmico en la vivienda social: análisis de una revisión sistemática prisma desde el diseño resiliente. Inteligencia artificial, problema u oportunidad para el Derecho. Impacto en el empleo en relación con las TIC y la inteligencia artificial. Neuroprivacidad. El test de asociación implícita, un paradigma que permite abordar nuestras actitudes inconscientes. ¿Cuál es el límite del uso de las tecnologías frente al derecho de información y de la libre expresión?. Tendencias de fijación de precios basados en el valor: un análisis desde la minería de datos. Recorrido virtual de la Universidad Católica de Colombia Sede Claustro para la inducción de estudiantes y docentes. Introducción a la bioarquitectura del paisaje, cartilla Paisaje, ambiente y tecnología. Descripción plan piloto (Choachí). Caracterización de las habilidades específicas para el reconocimiento del patrimonio cultural – Borde urbano sur oriental de Bogotá. Videojuego para estimular la memoria episódica en pacientes con deterioro cognitivo leve: validación de contenido. CONCLUSIONES

Published
2022

40. Targeting of plasmodesmal proteins requires unconventional signals.

Author

Luna, Gabriel Robles, Li, Jiefu, Wang, Xu, Liao, Li, and Lee, Jung-Youn

Subjects
*MEMBRANE proteins, *CELL communication, *ROOT development, *PROTEINS, *DISEASE resistance of plants, *CELL membranes, *NICOTIANA benthamiana
Abstract

For their delivery to plasmodesmata, plasmodesmal membrane proteins rely on a common targeting mechanism that requires unconventional signals located proximal to the membrane. Effective cellular signaling relies on precise spatial localization and dynamic interactions among proteins in specific subcellular compartments or niches, such as cell-to-cell contact sites and junctions. In plants, endogenous and pathogenic proteins gained the ability to target plasmodesmata, membrane-lined cytoplasmic connections, through evolution to regulate or exploit cellular signaling across cell wall boundaries. For example, the receptor-like membrane protein PLASMODESMATA-LOCATED PROTEIN 5 (PDLP5), a potent regulator of plasmodesmal permeability, generates feed-forward or feed-back signals important for plant immunity and root development. However, the molecular features that determine the plasmodesmal association of PDLP5 or other proteins remain largely unknown, and no protein motifs have been identified as plasmodesmal targeting signals. Here, we developed an approach combining custom-built machine-learning algorithms and targeted mutagenesis to examine PDLP5 in Arabidopsis thaliana and Nicotiana benthamiana. We report that PDLP5 and its closely related proteins carry unconventional targeting signals consisting of short stretches of amino acids. PDLP5 contains 2 divergent, tandemly arranged signals, either of which is sufficient for localization and biological function in regulating viral movement through plasmodesmata. Notably, plasmodesmal targeting signals exhibit little sequence conservation but are located similarly proximal to the membrane. These features appear to be a common theme in plasmodesmal targeting. [ABSTRACT FROM AUTHOR]

Published
2023
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42. 2020 taxonomic update for phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales

Author

National Institute of Allergy and Infectious Diseases (US), National Cancer Institute (US), National Institutes of Health (US), Frederick National Laboratory for Cancer Research (US), US Department of Homeland Security, National Institute of Food and Agriculture (US), Kuhn, Jens H. [0000-0002-7800-6045], Adkins, Scott [0000-0003-0095-8414], Alioto, Daniela [0000-0002-4954-8363], Alkhovsky, Sergey V. [0000-0001-6913-5841], Amarasinghe, Gaya K. [0000-0002-0418-9707], Avsic-Zupanc, Tatjana [0000-0001-6243-0688], Ayllón, María A. [0000-0001-7893-6106], Bahl, Justin [0000-0001-7572-4300], Balkema-Buschmann, A. [0000-0001-7613-9592], Ballinger, Matthew J. [0000-0001-7453-8725], Bartonička, Tomáš [0000-0001-7335-2435], Basler, Christopher F. [0000-0003-4195-425X], Bavari, Sina [0000-0001-8424-5635], Beer, Martin [0000-0002-0598-5254], Bente, Dennis A. [0000-0002-5150-7368], Bergeron, Éric [0000-0003-3398-8628], Bird, Brian H. [0000-0001-8170-8710], Blair, Carol D. [0000-0002-2803-0354], Blasdell, Kim R. [0000-0003-2121-0376], Bradfute, Steven B. [0000-0002-1985-751X], Breyta, Rachel [0000-0002-9106-1014], Briese, Thomas [0000-0002-4819-8963], Brown, Paul A. [0000-0002-6697-7688], Buchholz, Ursula J. [0000-0002-2329-3198], Buchmeier, Michael J. [0000-0002-2387-9287], Bukreyev, Alexander [0000-0002-0342-4824], Burt, Felicity [0000-0002-7238-7799], Buzkan, Nihal [0000-0002-0428-0447], Calisher, Charles H. [0000-0003-0213-294X], Cao, Mengji [0000-0003-0396-262X], Casas, Inmaculada [0000-0003-1840-1198], Chamberlain, John [0000-0002-7387-6420], Chandran, Kartik [0000-0003-0232-7077], Charrel, Rémi N. [0000-0002-7675-8251], Chen, Biao [0000-0002-2450-9296], Chiumenti, Michela [0000-0002-8412-3037], Choi, Il-Ryong [0000-0002-8035-7573], Clegg, J Christopher S. [0000-0003-3765-1862], Crozier, Ian [0000-0003-2485-4011], da Graça, John V. [0000-0003-0743-1519], Dávila, Alberto M.R. [0000-0002-6918-7673], de la Torre, J. C. [0000-0002-8171-8115], de Lamballerie, Xavier [0000-0001-7895-2720], De Swart, Rik L. [0000-0003-3599-8969], Di Bello, Patrick L. [0000-0002-4773-8040], Di Paola, Nicholas [0000-0002-9694-3913], Di Serio, Francesco [0000-0003-2822-704X], Dietzgen, Ralf G. [0000-0002-7772-2250], Digiaro, Michele [0009-0006-3089-3606], Dolja, Valerian V. [0000-0002-8148-4670], Dolnik, Olga [0000-0001-7739-4798], Drexler, J. Felix [0000-0002-3509-0232], Dürrwald, Ralf [0000-0002-3432-0438], Dufkova, Lucie [0000-0001-7302-2057], Dundon, William G. [0000-0002-2776-169X], Duprex, W. Paul [0000-0003-1716-6376], Dye, John M. [0000-0002-0883-5016], Easton, Andrew J. [0000-0002-2288-3882], Ebihara, Hideki [0000-0002-2576-9735], Elbeaino, Toufic [0000-0003-2211-7907], Ergünay, Koray [0000-0001-5422-1982], Fernandes, Jorlan [0000-0002-5039-0604], Fooks, Anthony R. [0000-0002-3243-6154], Formenty, Pierre B.H. [0000-0002-9482-5411], Forth, Leonie F [0000-0002-2708-2515], Fouchier, Ron A.M. [0000-0001-8095-2869], Freitas-Astúa, Juliana [0000-0002-0506-6880], Gago-Zachert, Selma [0000-0002-1084-2119], Gāo, George Fú [0000-0002-3869-615X], García, María Laura [0000-0001-6468-6943], García-Sastre, Adolfo [0000-0002-6551-1827], Garrison, Aura R. [0000-0003-3034-6400], Gbakima, Aiah [0000-0002-7642-0752], Goldstein, Tracey [0000-0002-1672-7410], Gonzalez, Jean-Paul J. [0000-0003-3063-1770], Griffiths, Anthony [0000-0001-5435-8364], Groschup, M. H. [0000-0003-0215-185X], Günther, Stephan [0000-0002-6562-0230], Guterres, Alexandro [0000-0001-8323-1477], Hall, Roy A. [0000-0001-5778-1387], Hammond, John [0000-0001-7593-747X], Hassan, Mohamed [0000-0002-1289-0878], Hepojoki, Jussi [0000-0001-5699-214X], Hepojoki, Satu [0000-0002-0696-1879], Hetzel, Udo [0000-0001-9142-560X], Hewson, Roger [0000-0003-2273-3152], Höper, Dirk [0000-0001-8408-2274], Horie, Masayuki [0000-0003-4682-7698], Hyndman, Timothy [0000-0002-7047-5023], Hughes, Holly R. 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Murilo [0000-0001-8617-0200], Kuhn, Jens H., Adkins, Scott, Alioto, Daniela, Alkhovsky, Sergey V., Amarasinghe, Gaya K., Anthony, Simon J., Avsic-Zupanc, Tatjana, Ayllón, María A., Bahl, Justin, Balkema-Buschmann, A., Ballinger, Matthew J., Bartonička, Tomáš, Basler, Christopher F., Bavari, Sina, Beer, Martin, Bente, Dennis A., Bergeron, Éric, Bird, Brian H., Blair, Carol D., Blasdell, Kim R., Bradfute, Steven B., Breyta, Rachel, Briese, Thomas, Brown, Paul A., Buchholz, Ursula J., Buchmeier, Michael J., Bukreyev, Alexander, Burt, Felicity, Buzkan, Nihal, Calisher, Charles H., Cao, Mengji, Casas, Inmaculada, Chamberlain, John, Chandran, Kartik, Charrel, Rémi N., Chen, Biao, Chiumenti, Michela, Choi, Il-Ryong, Clegg, J Christopher S., Crozier, Ian, da Graça, John V., Dal Bó, Elena, Dávila, Alberto M.R., de la Torre, J. C., de Lamballerie, Xavier, De Swart, Rik L., Di Bello, Patrick L., Di Paola, Nicholas, Di Serio, Francesco, Dietzgen, Ralf G., Digiaro, Michele, Dolja, Valerian V., Dolnik, Olga, Drebot, Michael A., Drexler, J. Felix, Dürrwald, Ralf, Dufkova, Lucie, Dundon, William G., Duprex, W. Paul, Dye, John M., Easton, Andrew J., Ebihara, Hideki, Elbeaino, Toufic, Ergünay, Koray, Fernandes, Jorlan, Fooks, Anthony R., Formenty, Pierre B.H., Forth, Leonie F., Fouchier, Ron A.M., Freitas-Astúa, Juliana, Gago-Zachert, Selma, Gāo, George Fú, García, María Laura, García-Sastre, Adolfo, Garrison, Aura R., Gbakima, Aiah, Goldstein, Tracey, Gonzalez, Jean-Paul J., Griffiths, Anthony, Groschup, M. H., Günther, Stephan, Guterres, Alexandro, Hall, Roy A., Hammond, John, Hassan, Mohamed, Hepojoki, Jussi, Hepojoki, Satu, Hetzel, Udo, Hewson, Roger, Hoffmann, Bernd, Hongo, Seiji, Höper, Dirk, Horie, Masayuki, Hughes, Holly R., Hyndman, Timothy, Jambai, Amara, Jardim, Rodrigo, Jiāng, Dàohóng, Jin, Qi, Jonson, Gilda B., Junglen, Sandra, Karadağ, Serpil, Keller, Karen E., Klempa, B., Klingström, Jonas, Kobinger, Gary, Kondō, Hideki, Koonin, Eugene V., Krupovic, Mart, Kurath, Gael, Kuzmin, Ivan V., Laenen, Lies, Lamb, Robert A., Lambert, Amy J., Langevin, Stanley L., Lee, Benhur, Lemos, Elba R.S., Leroy, Eric M., Li, Dexin, Li, Jianrong, Liang, Mifang, Liú, Wénwén, Liu, Yang, Lukashevich, Igor S., Maes, Piet, Marciel de Souza, William, Marklewitz, Marco, Marshall, Sergio H., Martelli, Giovanni P, Martin, Robert R., Marzano, Shin-Yi L., Massart, Sebastien, McCauley, John W., Mielke-Ehret, Nicole, Minafra, Angelantonio, Minutolo, Maria, Mirazimi, Ali, Mühlbach, Hans-Peter, Mühlberger, Elke, Naidu, Rayapati, Natsuaki, Tomohide, Navarro, Beatriz, Navarro, José A., Netesov, Sergey V., Neumann, Gabriele, Nowotny, N., Nunes, Márcio R.T., Nylund, Are, Okland, Arnfinn Lodden, Oliveira, Renata C., Palacios, Gustavo, Pallás, Vicente, Pályi, Bernadett, Papa, Anna, Parrish, Colin R., Pauvolid-Corrêa, Alex, Pawęska, Janusz T., Payne, Susan, Pérez, Daniel R., Pfaff, Florian, Radoshitzky, Sheli R., Rahman, Aziz-Ul, Ramos-González, Pedro L., Resende, Renato O., Reyes, Carina A., Rima, Bertus K., Romanowski, Víctor, Robles Luna, Gabriel, Rota, Paul, Rubbenstroth, Dennis, Runstadler, Jonathan A., Ruzek, Daniel, Sabanadzovic, Sead, Salát, Jiří, Sall, Amadou Alpha, Salvato, Maria S., Sarpkaya, Kamil, Sasaya, Takahide, Schwemmle, Martin, Shabbir, Muhammad Z., Shí, Xiǎohóng, Shí, Zhènglì, Shirako, Yukio, Simmonds, Peter, Širmarová, Jana, Sironi, Manuela, Smither, Sophie, Smura, Teemu, Song, Jin-Won, Spann, Kirsten, Spengler, Jessica R., Stenglein, Mark D., Stone, David M., Straková, Petra, Takada, Ayato, Tesh, Robert B., Thornburg, Natalie J., Tomonaga, Keizō, Tordo, Noël, Towner, Jonathan S., Turina, Massimo, Tzanetakis, Ioannis E., Ulrich, R. G., Vaira, Anna Maria, Van den Hoogen, Bernadette, Varsani, Arvind, Vasilakis, Nikos, Verbeek, Martin, Wahl, Victoria, Walker, Peter J., Wang, Hui, Wang, Jianwei, Wang, Xifeng, Wang, Lin-Fa, Wèi, Tàiyún, Wells, Heather, Whitfield, Anna E., Williams, John V., Wolf, Yuri I., Wú, Zhìqiáng, Yang, Xin, Yáng, Xīnglóu, Yu, Xuejie, Yutin, Natalya, Zerbini, F. Murilo, Zhang, Tong, Zhang, Yong-Zhen, Zhou, Guohui, and Zhou, Xueping

Abstract

In March 2020, following the annual International Committee on Taxonomy of Viruses (ICTV) ratification vote on newly proposed taxa, the phylum Negarnaviricota was amended and emended. At the genus rank, 20 new genera were added, two were deleted, one was moved, and three were renamed. At the species rank, 160 species were added, four were deleted, ten were moved and renamed, and 30 species were renamed. This article presents the updated taxonomy of Negarnaviricota as now accepted by the ICTV.

Published
2020

43. Carta de Psicología No. 55

Author

Romero-Rojas, David Camilo, Cobo-Charry, María Fernanda, Melo-López, Angie Lorena, Rueda-Rojas, Zaira Milena, Ardila-Ardila, Leidy Julieth, Cristancho, Yeimy Juliana, Alejo-Castañeda, Ivonne Edith, Manrique-Zuluaga, Tatiana, Almeida-Luna, Gabriel Eduardo, Vargas-Riaño, Nathaly Yurany, Sabogal-Carreño, Jenniffer Andrea, Casas-Soriano, David Santiago, Beltrán-Sotelo, Daniela, Martínez, Miguel Ángel, Romero, Olga, Ramírez, Andrés Fernando, Saavedra, María, Barbosa, Daniela, Algarra, Natalia, Castañeda, Daniel, Gómez-Montoya, Laura Liliana, Ayala-Rodríguez, Nelly, Martínez-Patiño, Karent Valentina, Fajardo-Castro, Leady Viky, Javela-Ávila, Leidy Tatiana, Maldonado-Bejarano, Angie Johana, Jiménez-Vanegas, Daniel Felipe, Rodríguez-Albañil, Duván Jair, Burgos-Agudelo, Camila Andrea, Gómez-Solano, Cristian Francisco, Córdoba-Pacheco, Nohora Gabriela, Pedraza-Quiñones, Natalia, García-Doroteo, Juan, Medina-Arboleda, Iván Felipe, Beltrán-Landázury, Jésica María, Grisales-Romero, Paula Ivonne, Nieto-Ramírez, María Fernanda, Torres-Pulido, Laura Andrea, Quitora-Vásquez, José Alejandro, León-Rincón, Darío, Martínez-Sarmiento, Daniela Alejandra, Triana-Jiménez, María Emily, Núñez-Flórez, Lisseht Lorena, González-González, Fernando Germán, Romero-Rojas, David Camilo, Cobo-Charry, María Fernanda, Melo-López, Angie Lorena, Rueda-Rojas, Zaira Milena, Ardila-Ardila, Leidy Julieth, Cristancho, Yeimy Juliana, Alejo-Castañeda, Ivonne Edith, Manrique-Zuluaga, Tatiana, Almeida-Luna, Gabriel Eduardo, Vargas-Riaño, Nathaly Yurany, Sabogal-Carreño, Jenniffer Andrea, Casas-Soriano, David Santiago, Beltrán-Sotelo, Daniela, Martínez, Miguel Ángel, Romero, Olga, Ramírez, Andrés Fernando, Saavedra, María, Barbosa, Daniela, Algarra, Natalia, Castañeda, Daniel, Gómez-Montoya, Laura Liliana, Ayala-Rodríguez, Nelly, Martínez-Patiño, Karent Valentina, Fajardo-Castro, Leady Viky, Javela-Ávila, Leidy Tatiana, Maldonado-Bejarano, Angie Johana, Jiménez-Vanegas, Daniel Felipe, Rodríguez-Albañil, Duván Jair, Burgos-Agudelo, Camila Andrea, Gómez-Solano, Cristian Francisco, Córdoba-Pacheco, Nohora Gabriela, Pedraza-Quiñones, Natalia, García-Doroteo, Juan, Medina-Arboleda, Iván Felipe, Beltrán-Landázury, Jésica María, Grisales-Romero, Paula Ivonne, Nieto-Ramírez, María Fernanda, Torres-Pulido, Laura Andrea, Quitora-Vásquez, José Alejandro, León-Rincón, Darío, Martínez-Sarmiento, Daniela Alejandra, Triana-Jiménez, María Emily, Núñez-Flórez, Lisseht Lorena, and González-González, Fernando Germán

Published
2022

44. XIII Jornada de Investigación 2022

Author

Barrios-Rodríguez, Cristian Bernardo, Martín-Fiorino, Víctor Rafael, Guadarrama-González, Pablo Manuel, Cortés-Ospina, Daiani Neriad, Díaz-Rico, Sara, Santacoloma-Suárez, Andrés Mauricio, Jiménez-Valderrama, Fernando Augusto, Quintero-Mora, Jasbleydy Victoria, Palacios-Ramos, Magda Alejandra, López-Ruiz, Paola Andrea, Rubiano-Cortés, Melissa María, Gutiérrez-Melo, Michel Dayana, González-Naranjo, Juan David, Aguilar-Pardo, David Ricardo, Muñoz-Obregón, María Alejandra, Romero-Aguilera, David Esteban, Nonsoque-Ruiz, Santiago José, González-Rojas, Camilo, Suárez-Sánchez, Nicole Nahom, Bonilla-Ávila, Dayanna Carolina, Rico-Melo, Gishet Mariana, Zúñiga-Barros, Iván Antonio, Tole-Acosta, Henry David, Acosta-Cipagauta, Christian Johnatan, Vallejo-Cagueñas, Jharol Orlando, Martínez-Pardo, Daniel Stiven, García-Paiba, Deyvi Felipe, Pérez-Riveros, José Miguel, Caicedo-Medina, Andrés Felipe, Solórzano, Indira Daniela, Corredor-Patiño, Laura Valentina, Pinilla-Triana, Luna Estefany, Gómez-Duarte, Javier Felipe, Arévalo-Muñetón, María Paula, Bastidas-Suárez, Douglas Mauricio, Ramírez-León, Lina Marcela, Barrios-Alfonso, Miguel Ángel, Romero-Babativa, Laura Valentina, Martínez-Acosta, Karen Sofía, Salgado-Cadavis, Gonzalo, Adoryan-Machado, María Luiza, Orjuela-Hernández, Andrea, Sánchez, Marco, Medina-Carrión, Ángela María, Chaves-Wilches, Laura Daniela, Jiménez-Barrera, Lina Vanessa, Sierra-Zamora, Paola Alexandra, Gil-Millán, Diego Alejandro, Cortés-López, Yerson Fahir, Carmona-Donoso, Erika Johanna, Guerrero-Gutiérrez, Paola Andrea, Coba-León, Camilo Andrés, Álvarez-Ortiz, Héctor Fernando, Mateus-Poveda, Ana María, Nicolaysen-Sánchez, Hans, Cely-Carvajal, Valentina, Cubides-Cárdenas, Jaime, Tequia-Fonseca, Andrea Marcela, Malagón-Riaño, Angie Paola, Muñoz-Molina, Tatiana, Vergara-Baquero, May Steven, Santana-Sua, Andrea Lizeth, Garavito-García, Luisa Fernanda, Almeida-Luna, Gabriel Eduardo, Alejo-Castañeda, Ivonne Edith, Manrique-Zuluaga, Tatiana, Rodríguez-Bautista, Ana María, Ruiz, Laura Valentina, Bernal, María Paula, Arévalo, Daniela, Moreno-Garzón, Diana Catalina, Moreno-Ardila, Karen Vanessa, Londoño-Fernández, María Camila, Vivas-Becerra, Camilo Jesús Alejandro, Mejía-Parrado, Paula Stefani, Jiménez-Carranza, Claudia Constanza, Aguilar-Bustamante, María Constanza, García-Bernal de Moncada, Doris, Gómez-Pedraza, Mauricio Alexander, Meneses-Báez, Alba Lucía, Sierra-Martínez, Marge Alejandra, Ortiz-Garzón, Eliana Yvette, Da Silva-Carvalho, Marcelo, Basto-Urbina, Estefanny Pamela, Alarcón-Rodríguez, Oscar Mauricio, Ramírez-Cuastuza, Anna Gabriela, Casas-Matiz, Elvia Isabel, Reyes-González, Alberto, García-Delgado, Nicolás, Acosta-Peña, María Camila, Martínez-Parra, Miguel Ángel, Pulido-Escobar, Paola Andrea, Rey-Gómez, Nathalia Patricia, Rodríguez-Prieto, Astrid Carolina, Santamaría-Uribe, Laura Sofía, Carmona-Rodríguez, Nicolás Fernando, Redondo, Johan Manuel, Yunis-Gómez, María Alejandra, Abello-Ávila, Nataly, Daza-Fonseca, Mateo, Martínez-Ochoa, Andrés David, Rodríguez-Romero, Pablo Enrique, Pardo-Galvis, Diana Paola, Algarra-Martínez, Erika Natalia, Ramírez-Velandia, Andrés Fernando, Sanmartín-Galvis, María Camila, Cruz-Gutiérrez, Maribel, Gutiérrez-Gaona, Yuliana, Díaz-Acevedo, Valentina, Camargo-Rojas, Karol Andrea, Villalba-Novoa, María Paula, Zuleta-Munive, María José, Bernal-Castro, Karen Daniela, Zuluaga-Canay, Jessica Katherine, González-Ceballos, María Fernanda, Varón-Melo, Karen Gisell, López-Coy, Juan Pablo, Ospina-Ortiz, Mariana, Albarracín-Mariño, María Camila, Meneses-Herrera, Camilo Andrés, Franco-Burgos, Jorge Ricardo, Ramírez-Salazar, Yesica Liliana, Valero-Melo, Lizeth Natalia, Cagua-Marroquín, Juan Sebastián, Baquero-Maldonado, Viviana Alejandra, Herrera-Cano, Camilo Ernesto, Riveros-Franco, Juan Camilo, Pulido-Bermeo, María Camila, Gómez-Rivera, David Felipe, Slebi-Acevedo, Carlos José, Rojas-Bedoya, Daniela, Yépez-Arrieta, Álvaro Javier, Bolívar-Barón, Holman Diego, Mejía-Morales, Juan David, Carrillo-Barón, Joan Sebastián, Contreras-Cifuentes, Angie Tatiana, Ríos-Cruz, Sonia Gisela, Caicedo-Támara, Natalia, Domínguez-Rozo, Andrés David, Barrero-Calixto, Juan Carlos, Beltrán-Rojas, Maikol Javie, Montero-Hernández, César Augusto, Castañeda-Tarquino, Daniel Francisco, Chacón-Ruiz, Jhon Erick, Tabares-Chaparro, Bryan Arley, Jara-Pardo, Diana Paola, Linares-Contento, Angie Katherine, Fajardo-Prada, David Mauricio, Pardo-Angulo, Fredy Ernesto, Argüello-Pintor, María Daniela, Nieto-Barbosa, Valentina, Tibério-Cardoso, Grace, Martínez-Rodríguez, Madeline Sofía, Carvajal-Builes, Juan Camilo, García-Ramírez, Laura Daniela, Avendaño-Prieto, Bertha Lucía, Barrios-Rodríguez, Cristian Bernardo, Martín-Fiorino, Víctor Rafael, Guadarrama-González, Pablo Manuel, Cortés-Ospina, Daiani Neriad, Díaz-Rico, Sara, Santacoloma-Suárez, Andrés Mauricio, Jiménez-Valderrama, Fernando Augusto, Quintero-Mora, Jasbleydy Victoria, Palacios-Ramos, Magda Alejandra, López-Ruiz, Paola Andrea, Rubiano-Cortés, Melissa María, Gutiérrez-Melo, Michel Dayana, González-Naranjo, Juan David, Aguilar-Pardo, David Ricardo, Muñoz-Obregón, María Alejandra, Romero-Aguilera, David Esteban, Nonsoque-Ruiz, Santiago José, González-Rojas, Camilo, Suárez-Sánchez, Nicole Nahom, Bonilla-Ávila, Dayanna Carolina, Rico-Melo, Gishet Mariana, Zúñiga-Barros, Iván Antonio, Tole-Acosta, Henry David, Acosta-Cipagauta, Christian Johnatan, Vallejo-Cagueñas, Jharol Orlando, Martínez-Pardo, Daniel Stiven, García-Paiba, Deyvi Felipe, Pérez-Riveros, José Miguel, Caicedo-Medina, Andrés Felipe, Solórzano, Indira Daniela, Corredor-Patiño, Laura Valentina, Pinilla-Triana, Luna Estefany, Gómez-Duarte, Javier Felipe, Arévalo-Muñetón, María Paula, Bastidas-Suárez, Douglas Mauricio, Ramírez-León, Lina Marcela, Barrios-Alfonso, Miguel Ángel, Romero-Babativa, Laura Valentina, Martínez-Acosta, Karen Sofía, Salgado-Cadavis, Gonzalo, Adoryan-Machado, María Luiza, Orjuela-Hernández, Andrea, Sánchez, Marco, Medina-Carrión, Ángela María, Chaves-Wilches, Laura Daniela, Jiménez-Barrera, Lina Vanessa, Sierra-Zamora, Paola Alexandra, Gil-Millán, Diego Alejandro, Cortés-López, Yerson Fahir, Carmona-Donoso, Erika Johanna, Guerrero-Gutiérrez, Paola Andrea, Coba-León, Camilo Andrés, Álvarez-Ortiz, Héctor Fernando, Mateus-Poveda, Ana María, Nicolaysen-Sánchez, Hans, Cely-Carvajal, Valentina, Cubides-Cárdenas, Jaime, Tequia-Fonseca, Andrea Marcela, Malagón-Riaño, Angie Paola, Muñoz-Molina, Tatiana, Vergara-Baquero, May Steven, Santana-Sua, Andrea Lizeth, Garavito-García, Luisa Fernanda, Almeida-Luna, Gabriel Eduardo, Alejo-Castañeda, Ivonne Edith, Manrique-Zuluaga, Tatiana, Rodríguez-Bautista, Ana María, Ruiz, Laura Valentina, Bernal, María Paula, Arévalo, Daniela, Moreno-Garzón, Diana Catalina, Moreno-Ardila, Karen Vanessa, Londoño-Fernández, María Camila, Vivas-Becerra, Camilo Jesús Alejandro, Mejía-Parrado, Paula Stefani, Jiménez-Carranza, Claudia Constanza, Aguilar-Bustamante, María Constanza, García-Bernal de Moncada, Doris, Gómez-Pedraza, Mauricio Alexander, Meneses-Báez, Alba Lucía, Sierra-Martínez, Marge Alejandra, Ortiz-Garzón, Eliana Yvette, Da Silva-Carvalho, Marcelo, Basto-Urbina, Estefanny Pamela, Alarcón-Rodríguez, Oscar Mauricio, Ramírez-Cuastuza, Anna Gabriela, Casas-Matiz, Elvia Isabel, Reyes-González, Alberto, García-Delgado, Nicolás, Acosta-Peña, María Camila, Martínez-Parra, Miguel Ángel, Pulido-Escobar, Paola Andrea, Rey-Gómez, Nathalia Patricia, Rodríguez-Prieto, Astrid Carolina, Santamaría-Uribe, Laura Sofía, Carmona-Rodríguez, Nicolás Fernando, Redondo, Johan Manuel, Yunis-Gómez, María Alejandra, Abello-Ávila, Nataly, Daza-Fonseca, Mateo, Martínez-Ochoa, Andrés David, Rodríguez-Romero, Pablo Enrique, Pardo-Galvis, Diana Paola, Algarra-Martínez, Erika Natalia, Ramírez-Velandia, Andrés Fernando, Sanmartín-Galvis, María Camila, Cruz-Gutiérrez, Maribel, Gutiérrez-Gaona, Yuliana, Díaz-Acevedo, Valentina, Camargo-Rojas, Karol Andrea, Villalba-Novoa, María Paula, Zuleta-Munive, María José, Bernal-Castro, Karen Daniela, Zuluaga-Canay, Jessica Katherine, González-Ceballos, María Fernanda, Varón-Melo, Karen Gisell, López-Coy, Juan Pablo, Ospina-Ortiz, Mariana, Albarracín-Mariño, María Camila, Meneses-Herrera, Camilo Andrés, Franco-Burgos, Jorge Ricardo, Ramírez-Salazar, Yesica Liliana, Valero-Melo, Lizeth Natalia, Cagua-Marroquín, Juan Sebastián, Baquero-Maldonado, Viviana Alejandra, Herrera-Cano, Camilo Ernesto, Riveros-Franco, Juan Camilo, Pulido-Bermeo, María Camila, Gómez-Rivera, David Felipe, Slebi-Acevedo, Carlos José, Rojas-Bedoya, Daniela, Yépez-Arrieta, Álvaro Javier, Bolívar-Barón, Holman Diego, Mejía-Morales, Juan David, Carrillo-Barón, Joan Sebastián, Contreras-Cifuentes, Angie Tatiana, Ríos-Cruz, Sonia Gisela, Caicedo-Támara, Natalia, Domínguez-Rozo, Andrés David, Barrero-Calixto, Juan Carlos, Beltrán-Rojas, Maikol Javie, Montero-Hernández, César Augusto, Castañeda-Tarquino, Daniel Francisco, Chacón-Ruiz, Jhon Erick, Tabares-Chaparro, Bryan Arley, Jara-Pardo, Diana Paola, Linares-Contento, Angie Katherine, Fajardo-Prada, David Mauricio, Pardo-Angulo, Fredy Ernesto, Argüello-Pintor, María Daniela, Nieto-Barbosa, Valentina, Tibério-Cardoso, Grace, Martínez-Rodríguez, Madeline Sofía, Carvajal-Builes, Juan Camilo, García-Ramírez, Laura Daniela, and Avendaño-Prieto, Bertha Lucía

Abstract

Los desafíos en las dinámicas económicas, sociales, políticas y psicológicas han puesto de relieve la importancia de involucrar en la enseñanza universitaria actividades que conecten a los estudiantes con las realidades del contexto en el que se desarrollan; simultáneamente, hoy más que nunca se hace evidente que la ciencia, la tecnología y la innovación (CTI) son cruciales para atender los retos sociales, ambientales y económicos de las sociedades actuales. En este contexto, la Jornada de Investigación de la Universidad Católica de Colombia es quizás uno de los espacios institucionales más emblemáticos en el que se visibilizan las actividades en CTI de estudiantes, jóvenes investigadores y profesores, que buscan contribuir a la solución de problemáticas relevantes del entorno. En esta oportunidad, aproximadamente 177 autores y más de 250 espectadores se dieron cita en un escenario virtual que permitió el intercambio de saberes y conocimientos en torno a muchos temas con un lenguaje común: el bienestar de la humanidad y la respuesta efectiva a los retos que tenemos como comunidad. Esta cuarta versión de las Memorias compila las ponencias presentadas en la XIII Jornada de Investigación de 2022, enmarcadas en los tres ejes temáticos de investigación que tiene la Universidad Católica de Colombia: i) Derecho, Cultura y Sociedad, ii) Desarrollo Humano y Sostenible, y iii) Gestión de la Tecnología al Servicio de la Sociedad. Desde la Dirección Central de Investigaciones nos encontramos profundamente agradecidos con todos y cada uno de los participantes, y nos sentimos aún más orgullosos por la calidad de los trabajos presentados. Sea esta la oportunidad para hacer extensiva una felicitación a los autores y a las diferentes Unidades Académicas que, con su compromiso e invaluable labor, permitieron que este evento se desarrollara con éxito.

Published
2022

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