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The purpose of the study is to reveal the role and place of traditions in Japanese animation.

Fusarium chuoi R. Hill, Gaya, D.T. Vu, Sand.-Den. & Crous, sp. nov. Etymology. From chu���i, Vietnamese vernacular name for Musa spp., from which the ex-type strain was isolated. Classification ��� Nectriaceae, Hypocreales, Sordariomycetes. On SNA and CLA, sporulation abundant from aerial conidiophores and sporodochia. Aerial conidiophores erect or prostrate, copiously branching laterally and sympodially, giving rise to macro-, and rarely, microconidia; aerial conidiogenous cells mono- and polyphialidic, subulate to subcylindrical, smooth- and thin-walled, proliferating sympodially, 6.5���40.5 �� 2.5���4 ��m, with apical flared collarette and periclinal thickening; aerial conidia of two types: microconidia often produced on prostrate conidiophores, rarely on aerial mycelium, aggregating in false heads, ellipsoidal, subcylindrical to slightly falcate, 0 ���1-septate, 8���15 �� 2���29.5 ��m; macroconidia fusiform to falcate, straight to apically dorsiventrally curved, apex curved to pointed, base obtuse to papillate, 1���3-septate, smooth- and thin-walled; 1-septate conidia: (14���)18���27.5(���29.5) �� (2.5���)3���4 ��m (av. 22.8 �� 3.2 ��m); 2-septate conidia: 26���28.5 �� 3���4 ��m (av. 27.4 �� 3.6 ��m); 3-septate conidia: (28���)31.5���43(���50.5) �� 3���4 ��m (av. 37.3 �� 3.5 ��m). Sporodochia saffron, luteous to ochreous coloured (Rayner 1970), formed abundantly on the agar surface and carnation leaves under nuv. Conidiophores in sporodochia, densely and irregularly branched, bearing apical whorls of 2 ��� 4 monophialides; sporodochial monophialides subcylindrical, 10���26 �� 2.5���4.5 ��m, smooth- and thin-walled, with a distinct apical collarette. Sporodochial conidia (macroconidia) falcate, almost straight to gently curved, tapering at both ends, apex curved to blunt, base poorly- to well-developed foot-shaped, 1���6-septate, hyaline, smooth- and thin-walled; 1-septate conidia: (14.5���)15���20.5(���24) �� 3���4.5 ��m (av. 17.9 �� 3.9 ��m); 2-septate conidia: 21.5���32 �� 3���4.5 ��m (av. 26.4 �� 3.5 ��m); 3-septate conidia: (33���)43���61(���71.5) �� (3���)4���5 ��m (av. 51.8 �� 4.2 ��m); 4-septate conidia: (50.5���)55���69(���74.5) �� 3.5���5 ��m (av. 62.3 �� 4.2 ��m); 5-septate conidia: 54 �� 4.5 ��m (rare); 6-septate conidia: (49.5���)56.5���71(���73) �� (3.5���)4���4.5(���5) ��m (av. 63.8 �� 4.3 ��m). Chlamydospores not observed. Culture characteristics ��� Colonies on potato dextrose agar (PDA) and oatmeal agar (OA) growing in the dark at 24 �� C covering and entire 9 cm Petri dish in 7 d. Colony surface peach to vinaceous, flat, velvety to felty with abundant floccose aerial mycelium forming concentric rings; colony margins undulate. Reverse flesh to salmon with diffuse coral to brick pigment throughout the medium. Typus. VIETNAM, H�� Tĩnh Province, H����ng S��n District,S��n Kim commune, N18��25'37.38" E105��12'53.95", inside seed of Musa itinerans (Musaceae), 9 Nov. 2014, D.M. Thu, L.T. Phong & T.T. Duong, isol. R. Hill (holotype CBS H-24901,culture ex-type CBS 148464; ITS, LSU, cmdA, rpb1, rpb2, tef1 and tub2 sequences GenBank OK586454, OK586452, OK626304, OK626306, OK626302, OK626308 and OK626310, MycoBank MB 841865). Colour illustrations. Flowers, fruits, leaves and seeds of Musa itinerans (background photo by D. T. Vu); from top to bottom and left to right: colony on PDA after 14 d at 24 �� C in darkness (left = obverse,right = reverse), sporodochia formed on CLA,aerial conidiophore,aerial conidiogenous cells,aerial conidia, sporodochial conidia. Scale bars: black = 20 ��m, white = 10 ��m. Additional material examined. VIETNAM, Ngh��� An Province, Con Cu��ng District, Ch��u Kh�� commune, N19��1'48.73" E104��43'31.97", inside seed of M. itinerans, 18 Nov. 2014, L.T. Phong, V.V. Tung & T.T. Duong, isol. R. Hill (culture CBS 148465; ITS, LSU, cmdA, rpb1, rpb2, tef1 and tub2 sequences GenBank OK586455, OK586453, OK626305, OK626307, OK626303, OK626309 and OK626311). Notes ��� Fusarium chuoi resides in the Asian clade of the Fusarium fujikuroi species complex (FFSC: O���Donnell et al. 1998, Yilmaz et al. 2021, Crous et al. 2021b). Based on nucleotide searches using the Fusarium Pairwise ID engine on the Fusarioid-ID database (www.fusarium.org, Crous et al. 2021) the closest hit using the ITS sequence was Fusarium siculi (strain CBS 142422; identities = 449/450 (99 %), no gaps). The closest hit using the LSU sequence was F. siculi (strain CBS 142422; identities = 804/805 (99 %), no gaps). Closest hit using the cmdA sequence was Fusarium fractiflexum (strain NRRL 28852; identities = 426/434 (98 %), no gaps). Closest hit using the rpb1 sequence was F. fujikuroi (strain NRRL 13566; identities = 687/702 (98 %), no gaps). Closest hit using the rpb2 sequence was Fusarium globosum (strain CBS 428.97; identities = 856/867 (98 %), no gaps). Closest hit using the tef1 sequence was F. fractiflexum (strain NRRL 28852; identities = 619/643 (96 %), 2 gaps (0.3 %)). The phylogenetic results, however, showed that F. chuoi is not directly related to any of the previously described species of FFSC (see Suppl. material FP1353), clustering as the second basal-most species of that clade after F. sacchari. Asian Fusarium spp. in the FFSC are characterised by mono-and polyphialides producing oval to ellipsoid, rarely pyriform to globose (i.e., F. annulatum, F. fujikuroi and F. globosum) microconidia organized in chains or false heads; 3���5-septate sporodochial conidia and lacking chlamydospores. The elaborate, profusely branched aerial conidiophores of F. chuoi are comparable to those of F. concentricum, F. lumajangense, F. mangiferae and F. sacchari, all the latter species producing oval, ellipsoidal to allantoid microconidia on false heads.Aerial conidiophores of F. chuoi, however, mostly produce macroconidia, while microconidia grouped on false heads are restricted to short, mostly unbranched and prostrate conidiophores formed on the surface on the culture media. Several Asian species of the FFSC have been reported from Musa spp. i.e., F. annulatum, F. concentricum, F. fujikuroi, F. lumajangense and F. sacchari (Leslie & Summerell 2006, Maryani et al. 2019, Farr & Rossman 2021). The two strains representing F. chuoi were isolated as endophytes from asymptomatic seeds of wild banana (Musa itinerans), which had been collected predispersal and stored in the Millennium Seed Bank for ~2.5 years at -20 �� C prior to isolation. Supplementary material FP1353 Phylogenetic tree., Published as part of Crous, P. W., Osieck, E. R., Jurjevi, ��, Boers, J., Van Iperen, A. L., Starink-Willemse, M., Dima, B., Balashov, S., Bulgakov, T. S., Johnston, P. R., Morozova, O. V., Pinruan, U., Sommai, S., Alvarado, P., Decock, C. A., Lebel, T., McMullan-Fisher, S., Moreno, G., Shivas, R. G., Zhao, L., Abdollahzadeh, J., Abrinbana, M., Ageev, D. V., Akhmetova, G., Alexandrova, A. V., Alt��s, A., Amaral, A. G. G., Angelini, C., Anton��n, V., Arenas, F., Asselman, P., Badali, F., Baghela, A., Ba��ares, A., Barreto, R. W., Baseia, I. G., Bellanger, J. - M., Berraf-Tebbal, A., Biketova, A. Yu., Bukharova, N. V., Burgess, T. I., Cabero, J., C��mara, M. P. S., Cano-Lira, J. F., Ceryngier, P., Ch��vez, R., Cowan, D. A., de Lima, A. F., Oliveira, R. L., Denman, S., Dang, Q. N., Dovana, F., Duarte, I. G., Eichmeier, A., Erhard, A., Esteve-Ravent��s, F., Fellin, A., Ferisin, G., Ferreira, R. J., Ferrer, A., Finy, P., Gaya, E., Geering, A. D. W., Gil-Dur��n, C., Gl��ssnerov��, K., Glushakova, A. M., Gramaje, D., Guard, F. E., Guarnizo, A. L., Haelewaters, D., Halling, R. E., Hill, R., Hirooka, Y., Hubka, V., Iliushin, V. A., Ivanova, D. D., Ivanushkina, N. E., Jangsantear, P., Justo, A., Kachalkin, A. V., Kato, S., Khamsuntorn, P., Kirtsideli, I. Y., Knapp, D. G., Kochkina, G. A., Koukol, O., Kov��cs, G. M., Kruse, J., Kumar, T. K. A., Ku��an, I., L��ss��e, T., Larsson, E., Lebeuf, R., Levic��n, G., Loizides, M., Marinho, P., Luangsa-ard, J. J., Lukina, E. G., Maga��a-Due��as, V., Maggs-K��lling, G., Malysheva, E. F., Malysheva, V. F., Mart��n, B., Mart��n, M. P., Mato��ec, N., McTaggart, A. R., Mehrabi-Koushki, M., Me��i��, A., Miller, A. N., Mironova, P., Moreau, P. - A., Morte, A., M��ller, K., Nagy, L. G., Nanu, S., Navarro-R��denas, A., Nel, W. J., Nguyen, T. H., N��brega, T. F., Noordeloos, M. E., Olariaga, I., Overton, B. E., Ozerskaya, S. M., Palani, P., Pancorbo, F., Papp, V., Paw��owska, J., Pham, T. Q., Phosri, C., Popov, E. S., Portugal, A., Po��ta, A., Reschke, K., Reul, M., Ricci, G. M., Rodr��guez, A., Romanowski, J., Ruchikachorn, N., Saar, I., Safi, A., Sakolrak, B., Salzmann, F., Sandoval-Denis, M., Sangwichein, E., Sanhueza, L., Sato, T., Sastoque, A., Senn-Irlet, B., Shibata, A., Siepe, K., Somrithipol, S., Spetik, M., Sridhar, P., Stchigel, A. M., Stuskova, K., Suwannasai, N., Tan, Y. P., Thangavel, R., Tiago, I., Tiwari, S., Tkal��ec, Z., Tomashevskaya, M. A., Tonegawa, C., Tran, H. X., Tran, N. T., Trov��o, J., Trubitsyn, V. E., Van Wyk, J., Vieira, W. A. S., Vila, J., Visagie, C. M., Vizzini, A., Volobuev, S. V., Vu, D. T., Wangsawat, N., Yaguchi, T., Ercole, E., Ferreira, B. W., de Souza, A. P., Vieira, B. S. & Groenewald, J. Z., 2021, Fusarium chuoi R. Hill, Gaya, D. T. Vu, Sand. - Den. & Crous, R. Hill, Gaya, D. T. Vu, Sand. - Den. & Crous sp. nov., pp. 310-311 in Fungal Planet 47 (1) on page 311, DOI: 10.5281/zenodo.5856199, {"references":["Rayner RW. 1970. A Mycological Colour Chart. Commonwealth Mycological Institute, Kew and British Mycological Society.","O'Donnell K, Cigelnik E, Nirenberg H. 1998. Molecular systematics and phylogeography of the Gibberella fujikuroi species complex. Mycologia 90: 465 - 493.","Yilmaz N, Sandoval-Denis M, Lombard L, et al. 2021. Redefining species limits in the Fusarium fujikuroi species complex. Persoonia 46: 129 - 162.","Crous PW, Lombard L, Sandoval-Denis M, et al. 2021 b. Fusarium: more than a node or a foot-shaped basal cell. Studies in Mycology 98: 100116.","Crous PW, Cowan DA, Maggs-Kolling G, et al. 2021 a. Fungal Planet descrip- tion sheets: 1182 - 1283. Persoonia 46: 313 - 528.","Leslie JF, Summerell BA. 2006. The Fusarium laboratory manual. Blackwell Publishing, Ames.","Maryani N, Sandoval-Denis M, Lombard L, et al. 2019. New endemic Fusarium species hitch-hiking with pathogenic Fusarium strains causing Panama disease in small-holder banana plots in Indonesia. Persoonia 43: 48 - 69."]}

At the Novosibirsk Chemical Concentrates Plant, the determination of alkali metals and calcium in the range of 0.1–100 mg/l is carried out using atomic emission spectra excited in an air-acetylene flame and recorded in the range of 390–860 nm. When these elements are analyzed simultaneously in a wide range of concentrations, the accumulation time of the analytical signal (exposure) is determined by the minimum concentration of calcium. However, at this exposure, the highly sensitive lines of sodium and potassium do not provide the analysis in the entire concentration range, since at large values, the analytical signal goes into saturation. Other analytical lines of potassium and sodium in the above-mentioned operating spectral range have three to four orders of magnitude lower sensitivity. As a result, there may be a gap in the range of detectable concentrations. This paper provides a way to eliminate this gap. To evaluate the degree of correction of the recorded signal, the relative intensities of the spectral lines of potassium and sodium excited in an air-acetylene flame are calculated. Then, based on the calculation results, an optimal echelette diffraction grating with a blaze angle of 300 nm is selected, and a combination of filters is installed to equalize the recorded radiation. As a result, for sodium and potassium, for which the determination is carried out along two analytical lines, the calibration dependences have an overlap that includes at least two concentration values.Keywords: Alkali metals, calcium, flame photometry, diffraction grating, light filters, dynamic range of calibration curves DOI: http://dx.doi.org/10.15826/analitika.2021.25.4.003 I.A. Zarubin1, 2, 3, A.N. Putmakov1, 3, E.A. Lukina4, D.O. Selunin1, 3, аnd I.D. Burumov1, 3 1Institute of Automation and Electrometry, Siberian Branch, Russian Academy of Sciences, pr. AkademikaKoptyuga 1, Novosibirsk, 630090, Russian Federation2Novosibirsk State Technical University, pr. K. Marksa 20, Novosibirsk, 630073, Russian Federation3VMK-Optoelektronika, pr. Koptyuga, 1, Novosibirsk, 630090, Russian Federation4Novosibirsk Chemical Concentrates Plant, ul. B. Khmelnitskogo, 94, Novosibirsk, 630110, Russian Federation, По атомно-эмиссионным спектрам, возбуждаемым в воздушно-ацетиленовом пламени и регистрируемым в диапазоне 390-860 нм, на Новосибирском заводе химконцентратов проводят определение 0.1-100 мг/л щелочных металлов и кальция. При их одновременном измерении в широком интервале концентраций время накопления аналитического сигнала определяется минимальной концентрацией кальция в пробе. Однако, при увеличении длительности регистрации сигнала, интенсивность высокочувствительных линий натрия и калия уходит в насыщение, что не позволяет обеспечить возможность их определения во всем необходимом диапазоне концентраций. Другие аналитические линии калия и натрия в указанной выше рабочей спектральной области обладают на 3-4 порядка меньшей чувствительностью. В результате этого возникает разрыв в диапазоне определяемых концентраций. В работе приводится способ устранения данного разрыва. Для оценки необходимой степени коррекции регистрируемого сигнала проведен расчет относительных интенсивностей спектральных линий калия и натрия, возбуждаемых в воздушно-ацетиленовом пламени. Далее, с учетом результатов расчета, была выбрана оптимальная дифракционная решетка-эшелетт, имеющая угол блеска для 300 нм, а также подобрана комбинация светофильтров для сближения интенсивностей регистрируемого излучения спектральных линий с резко различной чувствительностью. В результате для натрия и калия, у которых определение проводится по двум аналитическим линиям различной чувствительности, градуировочные зависимости имеют область перекрытия, включающую не менее двух значений концентрации.Ключевые слова. Щелочные металлы, кальций, пламенная фотометрия, дифракционная решетка, светофильтры, динамический диапазон градуировочных графиковDOI: http://dx.doi.org/10.15826/analitika.2021.25.4.003