90 results on '"Luiz Carlos Pinheiro Machado"'
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2. Caracterización de la composición florística del pastizal en el núcleo bovino en una finca de Florianópolis - SC, Brasil
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Yuseika Olivera-Castro, Maiara Mendes de Azevedo, Laura Livia Arias-Avilés, Luiz Carlos Pinheiro Machado Filho, and Pedro Pablo del Pozo-Rodriguez
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aplicación de abonos ,biodiversidad ,composición botánica ,Agriculture (General) ,S1-972 ,Animal culture ,SF1-1100 - Abstract
Objetivo Caracterizar la composición florística de los pastizales, después de dos años y medio de aplicar diferentes dosis de fertilizantes calcáreo, fosfórico y potásico en el núcleo bovino en una finca de Florianópolis - SC, Brasil. Materiales y Métodos La investigación se realizó en el núcleo bovino perteneciente a la hacienda Ressacada, de la Universidad Federal de Santa Catarina. Los tratamientos fueron 12, en los que se emplearon diferentes dosis de fertilización calcárea, fósforo y potasio. Para ello, se utilizó el método del marco del metro cuadrado, que se replicó cuatro veces en cada tratamiento. Resultados Se identificó la presencia de 19 familias con una amplia diversidad y 64 especies, la mayoría herbáceas. De manera general, casi todas las familias estuvieron presentes en los cuartones. Las de mayor representatividad fueron Poaceae, Cyperaceae y Fabaceae. Esta última con un aumento significativo con respecto al inicio del estudio, aunque todavía por debajo de los valores óptimos para tener cierto impacto en el pastizal. Conclusiones Se produjo un incremento de las leguminosas, y se encontró amplia diversidad de especies de plantas que los animales utilizan para su alimentación, representadas fundamentalmente por las familias Poaceae y Fabaceae.
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- 2020
3. Beef Cattle on Pasture Have Better Performance When Supplied With Water Trough Than Pond
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Gabriela Schenato Bica, Luiz Carlos Pinheiro Machado Filho, and Dayane Lemos Teixeira
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water intake ,average daily gain ,beef cattle welfare ,extensive pasture ,behavior ,Veterinary medicine ,SF600-1100 - Abstract
The behavior and performance of steers on pasture regarding water availability in troughs or in ponds were compared. Eight paddocks were randomly allocated to one treatment: POND (~30 m of diameter) or TROUGH (water trough, 120 cm diameter and 60 cm high and 500 L capacity). Eight groups of six beef steers were randomly assigned to one of the paddocks. The first 10 days were considered for animal habituation. Animals were individually weighed (days 0, 30, 60, and 90). Beginning in the day after each weighing on days 30 (Month 1), 60 (Month 2), and 90 (Month 3), behavior and animal distribution in the paddock were recorded by direct visual observation in three periods of 4 consecutive days. Water temperature and fecal and herbage DM were also recorded in these periods. Water intake was measured during 16 random days in the troughs. Data were analyzed using Generalized Linear Mixed Models, with treatment and period as fixed effects. TROUGH steers gained more weight (0.44 vs. 0.34 kg/day/animal; P ≤ 0.007) during the experiment and were heavier than the others at the end of the study (P ≤ 0.05). POND steers spent more time drinking water, but TROUGH steers increased the number of drinking events throughout the study (P ≤ 0.05), suggesting an adaptation for the new type of water source. Both treatments increased grazing time throughout the study, but not ruminating time (P ≤ 0.05). Walking time differed between treatments in all periods of behavior observation (P ≤ 0.05). Events of animal licking and ingesting salt of POND steers reduced throughout the study (P ≤ 0.05). The number of drinking events of TROUGH steers increased throughout the study, and drinking events were longer for POND steers than TROUGH steers (P ≤ 0.05). TROUGH steers spent more time on pasture on Month 2 (P ≤ 0.05). Period collection did not affect the water intake of TROUGH treatment (P > 0.05). This study demonstrates that water available in troughs rather than ponds for steers on pasture has positive effects on their weight gain and affects cattle behavior.
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- 2021
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4. The Equipment Used in the SF6 Technique to Estimate Methane Emissions Has No Major Effect on Dairy Cow Behavior
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Fabiellen Cristina Pereira, Dayane Lemos Teixeira, Laura Ann Boyle, Luiz Carlos Pinheiro Machado Filho, Shaun Richard Owen Williams, and Daniel Enriquez-Hidalgo
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cattle ,enteric methane ,milk production ,lying duration ,ruminating ,habituation ,Veterinary medicine ,SF600-1100 - Abstract
The natural behavior of animals can be disrupted by the techniques and materials of research methodologies. This study aimed to evaluate the effect of the equipment used in the SF6 tracer technique to estimate enteric methane emissions on the behavior of lactating dairy cows. The cows (n = 24) were allocated to one of two diets: CONTROL and experimental diet (MIX). Behavior was assessed through video recordings between milking times during four phases: 3 days before fitting the cows with the SF6 equipment (PRE), first 2 days after the cows were fitted with the SF6 equipment (ADAP), 3 days during methane emission measurements (MEAS), and 2 days after the SF6 equipment removal (POST). The behaviors recorded included eating, ruminating or idling, resting, and others. Affiliative or agonistic and discomfort behaviors (scratching or pushing the equipment) were also recorded. Lying time was recorded over 14 days using dataloggers fitted to the cows' leg. Milk production and feed intake were recorded daily. MIX cows ruminated more than CONTROL cows (P = 0.05). The cows ruminated more at MEAS than in any other phase (P < 0.01). Time spent idling gradually decreased from PRE to MEAS for MIX cows (P < 0.01). The cows were lying down longer in MEAS than in ADAP and POST (P < 0.01). The time spent lying with the head down was shorter during PRE and ADAP than during POST (P < 0.05). No difference was observed in the occurrence of discomfort or agonistic behaviors (P > 0.05). Affiliative behaviors occurred more often in ADAP than in MEAS (P < 0.05). There was no difference between phases in daily lying time, number of lying bouts per day, or mean bout duration (P > 0.05). Milk production was not influenced by the SF6 equipment (P > 0.05). Dry matter intake was higher for CONTROL cows (P < 0.01), and it decreased from PRE to MEAS (P < 0.01). However, milk yield did not differ between cows wearing the SF6 equipment and those without it (P > 0.05). We conclude that the SF6 equipment had a minimal effect on dairy cow behavior.
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- 2021
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5. Incorporating a Fresh Mixed Annual Ryegrass and Berseem Clover Forage Into the Winter Diet of Dairy Cows Resulted in Reduced Milk Yield, but Reduced Nitrogen Excretion and Reduced Methane Yield
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Daniel Enriquez-Hidalgo, Dayane Lemos Teixeira, Luiz Carlos Pinheiro Machado Filho, Deirdre Hennessy, Paula Toro-Mujica, Shaun Richard Owen Williams, and Fabiellen Cristina Pereira
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mixed herbage ,Trifolium alexandrinum ,Lolium multiflorum ,total mixed ration ,dairy cattle ,enteric methane ,Veterinary medicine ,SF600-1100 - Abstract
The winter diet of dairy cows in Mediterranean climate regions is usually a total mixed ration with a base of conserved summer crops such as corn silage and alfalfa hay. However, there is increased labor and financial cost related to this kind of feeding, which could be reduced if fresh forages were used in place of some of the conserved forage in the cow diet. The objective of our study was to evaluate the effect of including fresh mixed annual ryegrass and berseem clover into the diet of dairy cows on milk, nitrogen utilization, and methane emission. Twenty-four lactating dairy cows were split into two groups and offered either a diet similar to that usually offered to the cows (CON) or one where a mixture of fresh annual ryegrass and berseem clover was used to partially substitute the corn silage and alfalfa hay in the diet (MIX). Milk yield was recorded automatically, and methane emissions were estimated using the SF6 tracer technique. The MIX diet had lower crude protein concentration (148 vs. 170 g/kg DM) but higher DM digestibility (81.6 vs. 78.6%) than the CON diet. Compared to the cows offered the CON diet, milk yield was reduced when cows were fed the MIX diet (36.4 vs. 31.9 kg/d), but methane emissions (381 vs. 332 g/d) and nitrogen excretion were also reduced (238 vs. 180 g/d). Nitrogen use efficiency was unaffected (30.8%). In addition, milk from cows fed the MIX diet had a fatty acid profile considered to be more beneficial to human health than that of the milk from cows fed the CON diet. Increasing the protein concentration in the MIX diet, either by direct supplementation or increasing the proportion of legume in the mixed herbage, could overcome the reduction on milk and positively affect methane emission and N use efficiency.
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- 2020
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6. Black oat grown with common vetch improves the chemical composition and degradability rate of forage
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Fabiellen Cristina Pereira, Luiz Carlos Pinheiro Machado Filho, Daniele Cristina da Silva Kazama, and Roberto Guimarães Júnior
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cattle behavior ,grazing ,sustainability. ,Animal culture ,SF1-1100 - Abstract
This study aimed to evaluate the effect of cultivating a combination of common vetch (Vicia sativa L.) with black oats (Avena strigosa Schreb.) on the chemical composition of forage and the grazing behavior of heifers. To accomplish this, two paddocks 2500m2 each from a Voisin Rational Grazing management system were divided into three blocks each and then into thirds (278m²) characterizing a randomized block design. Three different forage compositions were distributed into these thirds: oats grown alone, vetch grown alone, and oats grown with vetch. Forage samples were collected after 65 days through the square method. Right after collection, three groups of four heifers each grazed the plots for two hours in a 3x3 double Latin Square design for behavioral observation, grazing simulation through the hand-plucking method, and biting rate determination. Forage samples collected either by hand-plucking or the square method, were analyzed for chemical composition and “in vitro” degradability. Statistical analyses were performed using the R package lme4. Data were evaluated with linear mixed-effects models. The inclusion of common vetch significantly increased forage production and oat protein content, but decreased the fiber content, which promoted better “in vitro” degradability. Grazing frequency was higher in pasture where oats were grown with vetch, but the biting rate was similar in all the three forage compositions evaluated. Forage collected by the square method did not differ from forage consumed by the heifers, probably meaning low herbage selectivity by heifers. Furthermore, no interaction of investigated variables occurred between forage compositions and the method of collection. The inclusion of common vetch with black oats increased forage chemical composition, “in vitro” degradability, and forage production, thus having positive effects on the time cows spent grazing.
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- 2020
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7. Time of Grain Supplementation and Social Dominance Modify Feeding Behavior of Heifers in Rotational Grazing Systems
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Gabriela Schenato Bica, Luiz Carlos Pinheiro Machado Filho, Dayane Lemos Teixeira, Karolini Tenffen de Sousa, and Maria José Hötzel
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social hierarchy ,cattle ,resources ,agonistic interactions ,subordinate ,Veterinary medicine ,SF600-1100 - Abstract
Social hierarchy affects the access of animals to feed resources. On daily rotational pasture systems, supplementation time may influence feeding behavior. This trial was designed to test the effect of grain delivery time on the feeding behavior of heifers. Heifers divided into two groups according to breed (n = 15 Braford and n = 19 Jersey) were tested in a crossover design with two treatments: INITIAL—supplement at 8 am (entry time to a fresh paddock), and MIDDLE—supplement at 4 pm (middle time of paddock use). Animals entered a new paddock every morning, and grain supplement at 2 kg/animal/day was offered at the fence line (1 m/animal). Then, ingestive and other behaviors were registered by direct visual observation through scan sampling at 2-min intervals for 1 h after grain supply. Agonistic interactions were recorded continuously (instigator–victim) to build a social matrix whereby each heifer was defined as dominant, intermediate, or subordinate. Weekly pasture samples were collected according to the order that animals left the feeding area, using the hand-plucking technique, to determine crude protein and fiber content. Heifers spent more time grazing on the INITIAL treatment (p < 0.0001) but exhibited more behaviors on the MIDDLE treatment (p < 0.0001). Dominant heifers spent more time eating grain (p = 0.0008), whereas subordinate heifers spent more time grazing along the paddock (p = 0.0067), but not along the fence (p = 0.0008). The crude protein content of pasture samples was higher for the INITIAL treatment (p < 0.0001). Behavioral interaction occurred with respect to the order of leaving the feeding area, social rank, and crude protein consumed (p = 0.04). Subordinate heifers consistently grazed more and ate less grain supplement than dominant and intermediate heifers. However, when grain supplement was offered at the time animals entered the paddock, more grazing activity took place during supplement feeding, and subordinate heifers could select a high-protein diet. In the INITIAL treatment, this means that subordinate animals could benefit from the better pasture available, keeping a distance from dominant heifers, reducing agonistic interactions and likely improving their welfare.
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- 2020
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8. Characterization of silage production and the use by dairy farmers in the West of Santa Catarina state
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Adenor Vicente Wendling and Luiz Carlos Pinheiro Machado Filho
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Maize cultivation ,Milk ,Pesticide ,Silage density. ,Agriculture (General) ,S1-972 - Abstract
Silage has been widely used in dairy production. However, little is known about its use at production level. The goal of this study was to characterize production and usage of silage for the dairy farming in Western Santa Catarina state. Thirty farms were selected randomly by their geographical location, in 11 municipalities of the West of Santa Catarina state, and they were invited to participate in the research. Out of these 30 farms, 6 did not have any dairy activity, and 2 preferred not to participate in the research, thus were not included. Semi-structured interviews and on-site visits were then performed. All data were analyzed with the software R version 3.2.2. 95% of the farmers produced and fed silage to animals. Out of the 22 farmers participating in the study, 82% provided silage for dairy cows during the whole year; in 86% of these farms, the silage was fed right after milking, in an average amount of 6.06 ± 0.63 kg DM (dry matter) AU-1 (animal unit, 500 kg) day-1. The average area used for maize production was 8.46 ha, representing 59% of the area destined to dairy farming. The average silage production was 8.8 and 7.6 t ha-1 year-1 in the season and off-season crop respectively, without significant differences. In most silage production areas, the no-tillage system is adopted, with the use of pesticides, chemical fertilization and transgenic seeds, without soil covering or crop rotation. Farms that fertilized maize based on soil analysis had the greatest silage yield, compared to the ones that fertilized without relying on soil analysis. The use of different types of pesticides and fertilizer quantities was not correlated to the silage production volume. Overall the production systems and the quantity of silage produced and fed to the cows do not follow technical criteria; thus silage yield and use could be improved if following strategies to better allocate crop inputs.
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- 2018
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9. Dairy Heifer Motivation for Access to a Shaded Area
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Clarissa Silva Cardoso, Marina A. G. von Keyserlingk, Luiz Carlos Pinheiro Machado Filho, and Maria José Hötzel
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operant conditioning ,behaviour ,heat stress ,animal welfare ,social rank ,Veterinary medicine ,SF600-1100 ,Zoology ,QL1-991 - Abstract
We used an operant conditioning paradigm to test the motivation of non-pregnant dairy heifers to access shade during the summer and autumn months (January to June) in southern Brazil. Dairy heifers (n = 18) were trained to push a weighted gate to access either an experimental area containing both a shaded (simple tree shade and shade cloth) and unshaded area (WITH SHADE) or an experimental area with no shade (BARREN). The latency to push the weighted gate, and the maximum weight pushed by each heifer, were recorded in both the summer and the autumn. Temperature and humidity were recorded continuously for the duration of the study and were used to calculate the heat index. The maximum weight pushed to enter the WITH SHADE area was greater in summer than in autumn, and was inversely related to the latency to push the weighted gate. Heifers refused to work for access to the BARREN environment. As expected, both the maximum ambient temperatures and heat index were higher in summer than in autumn, and also higher in the non-shaded areas than under the shade in both seasons. Heifers of higher social rank displaced other heifers more often, and spent more time in the shaded areas, particularly in the area with trees plus a shade cloth, than the intermediate and subordinate heifers. We conclude that shade is an important and valued resource for heifers reared on pasture-based systems in sub-tropical environments, particularly during the hot summer months.
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- 2021
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10. Social Licking in Pregnant Dairy Heifers
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Grazyne Tresoldi, Daniel M. Weary, Luiz Carlos Pinheiro Machado Filho, and Marina A. G. von Keyserlingk
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allogrooming ,affiliative behavior ,cattle welfare ,grazing ,competition. ,Veterinary medicine ,SF600-1100 ,Zoology ,QL1-991 - Abstract
Housing affects social behaviors, such as competition, but little work has addressed affiliative behaviors. This study compared social licking (SL) in pregnant heifers housed indoors (in a free-stall barn) versus outdoors (on pasture), and relationships with competition, feeding and physical proximity to others. Six heifer groups were observed during two six-hour-periods in both treatments. The total number of social events (SL and agonistic interactions) was four times higher when heifers were housed indoors compared to pasture (546 ± 43 vs. 128 ± 7 events/group; P < 0.05). SL as a ratio of the total number of social events was similar in the two treatments (12% vs . 8% of interactions, free-stall and pasture, respectively; P > 0.05). Housing did not affect how the SL bout was initiated and terminated, the duration, the body part licked and behavior preceding licking ( P > 0.05). Animals in close proximity showed higher rates of SL ( P < 0.0001) but not agonistic interactions ( P > 0.05). A previous agonistic event did not predict occurrence or the role of heifers in the following licking event. The higher stocking density indoors likely resulted in increased social interactions.
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- 2015
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11. Farm Management in Organic and Conventional Dairy Production Systems Based on Pasture in Southern Brazil and Its Consequences on Production and Milk Quality
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Shirley Kuhnen, Rudinei Butka Stibuski, Luciana Aparecida Honorato, and Luiz Carlos Pinheiro Machado Filho
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organic milk ,pasture-based dairy production ,sustainable agriculture ,food security ,Veterinary medicine ,SF600-1100 ,Zoology ,QL1-991 - Abstract
Pasture-based dairy production is used widely on family dairy farms in Southern Brazil. This study investigates conventional high input (C-HI), conventional low input (C-LI), and organic low input (O-LI) pasture-based systems and their effects on quantity and quality of the milk produced. We conducted technical site visits and interviews monthly over one year on 24 family farms (n = 8 per type). C-HI farms had the greatest total area (28.9 ha), greatest percentage of area with annual pasture (38.7%), largest number of lactating animals (26.2) and greatest milk yield per cow (22.8 kg·day−1). O-LI farms had the largest perennial pasture area (52.3%), with the greatest botanical richness during all seasons. Area of perennial pasture was positively correlated with number of species consumed by the animals (R2 = 0.74). Milk from O-LI farms had higher levels of fat and total solids only during the winter. Hygienic and microbiological quality of the milk was poor for all farms and need to be improved. C-HI farms had high milk yield related to high input, C-LI had intermediate characteristics and O-LI utilized a year round perennial pasture as a strategy to diminish the use of supplements in animal diets, which is an important aspect in ensuring production sustainability.
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- 2015
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12. Productive and Economic Responses in Grazing Dairy Cows to Grain Supplementation on Family Farms in the South of Brazil
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Luiz Carlos Pinheiro Machado Filho, Leandro Martins Dvila, Daniele Cristina da Silva Kazama, Lauana Luiza Bento, and Shirley Kuhnen
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livestock sustainability ,grazing behavior ,milk composition ,Voisin’s Rational Grazing ,Veterinary medicine ,SF600-1100 ,Zoology ,QL1-991 - Abstract
Pasture-based dairy production has been a major source of income for most family farms in the south of Brazil. Increasing milk prices have spurred an increase in grain supplementation, which has been poorly implemented, resulting in low levels of efficiency. To evaluate the consequences of supplementation on milk production and composition, grazing behavior and economic return, the widely used grain management system (CC-commercial concentrate, containing 21% CP, offered at 1 kg per 3.7 L of milk) was compared with an energy supplement (GC-ground corn, with 9.5% CP, offered at 0.4% of live weight). Ten Holstein cows were paired into two groups, and subjected to the two treatments in a crossover design. The cows remained in the same grazing group, and the grain supplement was offered individually at milking time and consumed completely. Each experimental period lasted 14 days, with 10 days for diet adaptation and four days for data collection; individual milk production and samples were collected to determine levels of fat, protein, lactose, carotenoids, vitamin A and N-urea. Grazing behavior was observed (scans every 5 min) in the first 4 h after the morning milking, and chemical composition of hand plucked samples of forage were measured. The cost of the supplement and profitability per treatment were calculated. Cows supplemented with GC consumed herbage with higher crude protein (CP: 16.23 vs. 14.62%; p < 0.05), had higher biting rate (44.21 vs. 39.54 bites/min; p < 0.03) and grazing time (22.20 vs. 20.55 scans; p < 0.05) than when receiving CC. There were no differences in milk composition between treatments (p > 0.05). However, higher concentrations of β-carotene and total carotenoids were detected in the milk of cows at 70–164 days of lactation, compared to
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- 2014
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13. Particularidades relevantes da interação humano-animal para o bem-estar e produtividade de vacas leiteiras Particularities of the human-animal interactions relevant to the welfare and productivity of dairy cows
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Luciana Aparecida Honorato, Maria José Hötzel, Carla Christina de Miranda Gomes, Isabella Dias Barbosa Silveira, and Luiz Carlos Pinheiro Machado Filho
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interação humano-animal ,comportamento ,bem-estar animal ,human-animal interactions ,behavior ,animal welfare ,Agriculture ,Agriculture (General) ,S1-972 - Abstract
Mudanças na qualidade da relação entre os animais e as pessoas podem influenciar substancialmente na produtividade e no bem-estar dos animais e, potencialmente, dos humanos envolvidos na atividade leiteira. Importantes alterações no sentido da melhoria dessas relações podem ser obtidas através de programas de treinamento, ou outras formas de extensão. Para que isso tenha efetividade, é necessário conhecer como interagem os aspectos dessa relação, que incluem além dos animais e os homens, o ambiente em que eles se inserem. A maioria dos estudos publicados confirma o modelo de retroalimentação positiva de atitudes e comportamentos humanos e comportamento dos animais. Porém, esses estudos têm sido desenvolvidos em países da Europa ou na Austrália, na maioria das vezes, sob condições de criação intensiva confinada e, geralmente, com o intuito de indicar um perfil ideal de empregado para trabalhar com os animais. Nesta revisão, mostra-se que o sistema de criação pode exercer uma forte interferência nesse processo e conclui-se sugerindo o desenvolvimento de estudos voltados a compreender as relações entre humanos e animais em diferentes sistemas de criação, enfocando as diversas realidades do nosso país. Esses estudos devem procurar entender de que modo características como o manejo alimentar e sanitário empregado, a qualidade das instalações, a genética, o tamanho e a composição dos rebanhos, e as peculiaridades culturais regionais influenciam na qualidade dessas relações.Changes in the quality of interactions between animals and humans have a profound influence on the productivity and welfare of animals and, potentially, of humans involved in the dairy activity. Important alterations in these interactions can be obtained through training programs, or other forms of extension. To do so effectively, it is necessary to understand how interact the several aspects of this relationship, which includes besides the animals humans, and the environment where they are inserted. Most of published studies confirm the model of positive feedback human behavior and attitudes, and animal behavior. However, these studies have been carried out mostly in European countries or Australia, mostly under intensive, confined systems, and usually with the aiming of indicating an ideal worker profile. In this review we show that the rearing system may exert a strong influence in this process and conclude suggesting the development of studies with the objective of understanding the relationships between humans and animals in different farming systems, focusing on the diverse realities of Brazil. These studies should search understanding how characteristics such as feeding and health management, quality of facilities, genetics, size and composition of herds, and cultural regional peculiarities, influence the quality of these relationships.
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- 2012
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14. Ethological aspects on water supply for dairy cattle
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José Daniel Cazale, Daniel Enriquez Hidalgo, Luiz Carlos Pinheiro Machado Filho, Dayane Lemos Teixeira, and Maria José Hötzel
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animal behavior ,animal welfare ,drinking behavior ,ethology ,milk production ,Science ,Biology (General) ,QH301-705.5 - Abstract
In order to identify appropriate alternatives for the provision of water to dairy cattle, the preference of dairy cows among three types of water troughs used in pasturebased systems was evaluated. The fi rst water trough was round, had a diameter of 120cm, was 60cm high and held 500L (500C); the second was a round water trough, 60cm in diameter, 60cm high, and held 125L (125C); the last trough was rectangular, 30cm high and 100cm long, and held 100L (100R). Individual preference tests were carried out with 17 dairy cows. The cows preferred to drink 67% of the time from the 500C trough, 18% of the time from the 100R trough, and 15% of the time from the 125C trough (p
- Published
- 2009
15. Comportamento de vacas leiteiras submetidas a um manejo aversivo
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Maria José Hötzel, Carla Christina de Miranda Gomes, and Luiz Carlos Pinheiro Machado Filho
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Gado de leite ,Relação humano-animal ,Distância de fuga ,Etologia ,Bem-estar animal ,Science ,Biology (General) ,QH301-705.5 - Abstract
Em pequenas propriedades leiteiras, devido à falta de instalações adequadas para o manejo, as vacas podem ser contidas e submetidas à inspeção ou tratamento veterinário no seu ambiente de ordenha, o que, por sua vez, pode influenciar o comportamento dos animais, perturbando a rotina de manejo. Sete vacas leiteiras, mantidas em sistema intensivo de pastoreio rotativo e ordenhadas duas vezes por dia por dois manejadores, foram expostas a um minucioso exame clínico, durante três dias consecutivos. Dados comportamentais antes e depois do procedimento foram analisados através de uma análise de variância. O comportamento das vacas durante o procedimento indicou forte aversividade. O tratamento não influenciou a distância de fuga mantida em relação ao veterinário ou a uma pessoa desconhecida pelas vacas, avaliadas antes e após o procedimento veterinário (veterinário: antes = 1,2±0,1; após 0,8±0,2; desconhecido: 1,0±0,2 após 1,2±0,2; p=0,3), nem o número de interações agonísticas no grupo, observadas antes (7,1±2) e após (11,5±3) o procedimento (p=0,3), ou um escore de reatividade atribuído a cada animal (p=0,2). Estes resultados não permitem concluir que a aplicação repetida e inevitável de procedimentos veterinários no ambiente da ordenha influencia o comportamento das vacas durante a ordenha ou a sua reatividade ao homem.
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- 2009
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16. [Untitled]
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Marcela Cristina Agustini Carneiro da Silveira, Luiz Carlos Pinheiro Machado Filho, and Maria José Hötzel
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Leite residual ,Mastite ,Bem-estar ,Ordenha ,Vaca leiteira ,Science ,Biology (General) ,QH301-705.5 - Abstract
O leite residual (LR) pós-ordenha é um importante fator predisponente de mastite. Neste estudo foi testada a hipótese de que a massagem do úbere ao final da ordenha pode reduzir o LR e a incidência de mastite. Para tanto foram realizados dois experimentos, o experimento 1 foi realizado numa fazenda experimental com 10 vacas (6.200kg/lactação) e o experimento 2 em fazenda comercial com 52 vacas (4.480kg/lactação). Em ambos os experimentos as vacas foram pareadas por número de partos, estágio de lactação e produtividade e aleatoriamente destinadas a um dos tratamentos: com massagem ou sem massagem do úbere, em duas etapas num desenho “cross-over”. A produção de leite individual de cada vaca foi medida. O leite residual foi coletado e medido 2min após aplicação de ocitocina intravenosa. Foi usado o Califórnia Mastite Teste (CMT) para detectar mastite subclínica. Em ambos os experimentos a massagem não teve efeito na produção de leite (experimento 1, p > 0,41, n = 10; e experimento 2, p > 0,46, n = 12), nem no LR (experimento 1, 1,78 ± 0,45kg, n = 10; experimento 2, 2,42 ± 0,32kg, n = 12), e nem na incidência de mastite (experimento 2, qui-quadrado, p > 0,68, n = 26). Concluiu-se que, nas condições do experimento, a massagem no úbere não teve efeito no LR e nem na incidência de mastite.
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- 2009
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17. Aspectos etológicos no suprimento de água em bovinos leiteiros
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Dayane Lemos Teixeira, Maria José Hötzel, Luiz Carlos Pinheiro Machado Filho, Daniel Enriquez Hidalgo, and José Daniel Cazale
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Bem-estar animal ,Comportamento animal ,Comportamento de beber ,Etologia ,Produção leiteira ,Science ,Biology (General) ,QH301-705.5 - Abstract
Com o intuito de avaliar três alternativas adequadas de oferta de água para vacas leiteiras, alimentadas a base de pasto, na região sul do Brasil, foram disponibilizados três tipos de bebedouros, sendo: (1) formato circular, com 60cm de altura x 120cm de diâmetro e capacidade para 500L (500C); (2) formato circular, com 60cm de altura x 60cm de diâmetro e capacidade para 125L (125C) e (3) formato retangular, com 30cm de altura x 100cm de comprimento x 30cm de largura e capacidade para 100L (100R). Os resultados obtidos através de testes individuais de preferência por parte das 17 vacas em lactação, mostraram 67% de acessos no bebedouro 500C, 18% no 100R e 15% no 125C (p
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- 2009
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18. Behaviour of dairy cows subjected to an aversive veterinary procedure.
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Maria José Hötzel, Carla Christina de Miranda Gomes, and Luiz Carlos Pinheiro Machado Filho
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dairy cattle ,human–animal relationship ,flight distance ,applied ethology ,animal welfare ,Science ,Biology (General) ,QH301-705.5 - Abstract
On small dairy farms that lack appropriate handling facilities, cows can be restrained and subjected to veterinary inspection or treatment in their milking environment, which in turn might influence the behaviour of the animals, disrupting routine management. A group of seven dairy cows kept on an intensive rotational pasture system and machine milked twice a day by two familiar handlers were exposed to a thorough clinical examination for three consecutive days. Behavioural data before and after the procedure were analyzed by ANOVA. The behaviour of all the cows during the procedure indicated strong aversiveness. Treatment did not influence the flight distance (metres) kept from the veterinarian or from a person unknown by the cows, assessed before and after the procedure (veterinarian: before = 1.2 ± 0.1; after 0.8 ± 0.2; unknown: 1.0 ± 0.2 after 1.2 ± 0.2; p=0.3), nor did it affect the number of agonistic interactions within the group observed before (7.1 ± 2) and after (11.5 ± 3) the procedure (p=0.3), or reactivity score (p=0.2). These results do not support the conclusion that the repeated application of unavoidable aversive veterinary procedures in the milking environment will influence the behaviour of cows during milking or their reactivity to humans.
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- 2009
19. Effect of udder massage at the end of milking on residual milk and mastitis occurrence in dairy cows.
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Marcela Cristina Agustini Carneiro da Silveira, Luiz Carlos Pinheiro Machado Filho, and Maria José Hötzel
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residual milk ,mastitis ,welfare ,management ,milking ,Science ,Biology (General) ,QH301-705.5 - Abstract
Residual milk (RM) after milking is an important factor that can predispose cows to mastitis. In this study, the hypothesis that udder massage at the end of milking may reduce RM and the incidence of mastitis was tested in two experiments. Experiment 1 was conducted on an experimental farm with ten lactating cows (6,200kg/lactation) and experiment 2 was carried out on a commercial farm with 52 lactating cows (4,480kg/lactation). In both experiments the cows were paired by parity, stage of lactation and productivity, and they were randomly assigned to one of the treatments: massage or no massage of the udder at the end of milking, in two periods in a crossover design. The individual milk production of each cow was measured. Residual milk was collected and measured 2min after an oxitocin application. California Mastitis Test was used to detect the occurrence (experiment 1 p > 0.41, n = 10 and experiment 2 p > 0.46, n = 12), on RM (experiment 1, 1.78 ± 0.45kg, n = 10; experiment 2, 2.42 ± 0.32kg, n = 12), or on mastitis incidence (experiment 2, chi-square; DF = 1; p > 0.68, n = 26). We conclude that udder massage at the end of milking has no effect on RM and does not affect mastitis incidence, at least under the conditions used in these experiments.
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- 2009
20. Stress and recognition of humans in weanling piglets
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Renato Irgang, Luiz Carlos Pinheiro Machado Filho, Gisele Pacheco de Souza, Maria José Hötzel, and Ricardo Probst
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fear ,human-animal interactions ,social behavior ,weaning ,swine ,Science ,Biology (General) ,QH301-705.5 - Abstract
This study was aimed at investigating whether after weaning, piglets recognize persons that have handled them aversively during the lactation period, and whether such treatment intensifies the stress of weaning. Before weaning, five litters received aversive handling treatment involving an aggressive and intimidating voice; six litters were treated conventionally. After weaning, the piglets’ behavior was compared in a series of tests. Compared to day 10 after weaning, in the first two days after weaning higher frequencies of escape attempts, vocalizations, and standing and sitting, accompanied by a lower frequency of feeding (p
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- 2007
21. Reproductive behaviour repertoire of semi-captive lowland tapir Tapirus terrestris (Linnaeus, 1758)
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Luisa Brusius, Luiz Carlos Pinheiro Machado Filho, Luiz Gustavo R. Oliveira-Santos, Marcos A. Tortato, and Maria J. Hötzel
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courtship ,tapir ,flehmen ,copulation ,reproductive behaviour ,Science ,Biology (General) ,QH301-705.5 - Abstract
The reproductive behaviour of five male and two female semi-captive Tapirus terrestris was investigated for two years without interruption. They were fenced in a 160 hectare area, in Santa Catarina State, South of Brazil. Data was collected whenever an animal exhibited reproductive behaviour. Seven behaviours, observed in eleven reproductive events, were divided into three categories: courtship, copulation and post-copulation. The tapirs displayed stereotypical patterns of sexual behaviour. No kind of audible vocalization was recorded during the reproductive period. All reproductive activity began in the second quarter of July and continued up to the first half of October, in both years of observation, pointing towards the existence of a reproductive season, corroborated by two births after expected gestation period from copula.
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- 2007
22. Foot and mouth disease eradication policy: social impact and animal welfare
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Barbara Marins Pettres, Luiz Carlos Pinheiro Machado, Maria José Hötzel, and Tania Maria de Paula Lyra
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Febre aftosa ,erradicação ,bem-estar animal ,políticas públicas ,Foot and mouth disease ,eradication ,animal welfare ,public policies ,Fiebre aftosa ,erradicación ,bienestar animal ,Social Sciences ,Social sciences (General) ,H1-99 - Abstract
Santa Catarina is the only Brazilian state that does not immunize the bovine herd against foot and mouth disease. This article discusses the policy adopted for the foot and mouth disease in Santa Catarina, especially the non-vaccination, and relates this policy with ethical, human and animal welfare issues. Nine representatives of agricultural institutions in the state were interviewed, as well as, in a case study, seven families of farmers in Jóia - Rio Grande do Sul, Brazil, where foot and mouth disease occurred in 2000, leading to the sacrifice of 11,067 animals, most of them dairy animals. The majority of the agricultural institutions in Santa Catarina are contrary to vaccination, in order to keep and extend pig and poultry export markets. Concerns on social repercussions tended to concentrate on the effects on the income of the affected families. The case study in Jóia demonstrated that the life styles of the affected farmers were deeply harmed due to effects on human mental health, loss of income and changes in the local economy. The study concludes that the experience of a foot and mouth disease outbreak results in traumatic and long term consequences and that there is a need for policies that include social, ethical and environmental provisions, once animal welfare aspects and impacts on other areas of the economy are not contemplated in the public policy of animal sanitary defense.
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- 2007
23. The effect of gastrointestinal nematode infection level on grazing distance from dung.
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Hizumi Lua Sarti Seó, Luiz Carlos Pinheiro Machado Filho, Luciana Aparecida Honorato, Bruna Fernanda da Silva, Alessandro Fernando Talamini do Amarante, and Patrizia Ana Bricarello
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Medicine ,Science - Abstract
Avoiding grazing near feces is an efficient strategy to prevent parasitic infection and contamination; therefore, in the evolution of herbivorous species, this behavior may have developed as a mechanism to protect the host against infection by gastrointestinal nematodes. The aim of this study was to assess whether grazing distance from dung is related to the level of parasitic infection in cattle. Based on Fecal Egg Count (FEC) means, 18 castrated male steers, aged 18 months, were divided into three groups: High (FEC ≥ 315); Medium (FEC = 130-160); and Low (FEC = 40-70). To analyze the response to a new natural infection by gastrointestinal nematodes and to standardize infection levels, all animals received anthelmintic treatment at twenty days prior to field observation. Three observers simultaneously collected data on grazing behavior for 2.5 hours/week for 12 weeks. Observers recorded the distance when grazing occurred at less than one meter from dung. Every two weeks, fecal samples were collected for FEC, as well as serum samples to measure immunoglobulin G (IgG) levels against larvae and adult antigens of the parasitic species Haemonchus placei. All groups grazed farther from the dung on days of greater insolation (r = 0.62; P = 0.03). Animals with high levels of parasitism grazed farther from the dung (P < 0.05) but had lower levels (P < 0.0001) of IgG serum levels compared to those with medium and low levels of infection. FEC values varied over the experiment, remaining below 200 for the low and medium group and reaching 1000 (P < 0.01) for the animals with the highest rates of parasitism. Our results indicate that cattle showing high levels of parasitism are more likely to avoid contaminated areas than animals with lower infection levels, and the immune system seems to be involved in such behavior.
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- 2015
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24. Influência de um ordenhador aversivo sobre a produção leiteira de vacas da raça Holandesa An aversive milker causes fear, but does not influence milk yield of Holstein cows
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Maria José Hötzel, Luiz Carlos Pinheiro Machado Filho, Maria Cristina Yunes, and Marcela Cristina A. C. da Silveira
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bem-estar animal ,bovino ,etologia ,medo ,relação humano-animal ,bovine ,ethology ,animal welfare ,human-animal interactions ,fear ,Animal culture ,SF1-1100 - Abstract
Neste estudo, foi testada a hipótese de que o medo de seres humanos é duradouro e pode prejudicar a produção leiteira de vacas da raça Holandesa. Um tratador aversivo realizou duas sessões diárias de um tratamento hostil (duas palmadas na região posterior e um grito forte), durante 21 dias, e um tratador neutro ordenhou as vacas, durante o mesmo período, não oferecendo nenhuma ameaça. A distância de fuga na presença do tratador aversivo e do neutro foi medida antes e 14 e 180 dias após o início do tratamento. As produções total e residual de leite (após aplicação i.v. de ocitocina na veia caudal) foram medidas a partir da ordenha realizada pelo tratador aversivo ou ou pelo neutro, entre os dias 14 e 24 do experimento. Todas as vacas (n=13) participaram dos dois tratamentos, constituindo um delineamento experimental do tipo "cross-over". Com exceção do período anterior ao experimento, as vacas mantiveram maior distância de fuga do tratador aversivo que do neutro, mesmo 180 dias após o último contato com os tratadores. Entretanto, não houve efeito do tratamento sobre a produção total ou residual de leite. Os resultados corroboram outros estudos que comprovaram que vacas discriminam tratadores aversivos de neutros. Entretanto, o tratamento aqui aplicado não afetou as produções total ou residual de leite.In the present study the hypothesis that fear of humans is long-lasting and may influence milk production in Holstein cows was tested. One person (the aversive handler) carried out two daily sessions of an aversive treatment during 21 days, where each cow received two slaps on the rump and a strong yell. The control handler walked around the cows not menacing the cows. Flight distance from both handlers was measured before the treatments were imposed and 14 and 180 days later. Total and residual milk (measured after an i.v. oxytocin injection in the caudal vein) were measured when either the aversive or the neutral handler carried out the milking, between days 14 and 24 of the experiment. All the cows were used in both treatments in a cross-over design. Except for the day before treatments started, the cows kept a larger distance from the aversive than from the neutral handler, even after 180 days without contact with these handlers. Nevertheless, there was no effect of treatment on total milk yield or residual milk. The results corroborate others that show that cows can discriminate between aversive and neutral individuals. Under the conditions of the present study, the fact that milking was done by the aversive individual did not influence total milk yield and residual milk.
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- 2005
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25. Behaviour of pre-parturient sows housed in intensive outdoor or indoor systems Comportamento de porcas pré-parturientes alojadas no sistema intensivo ao ar livre ou no confinamento convencional
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Maria José Hötzel, Luiz Carlos Pinheiro Machado Filho, and Osmar Antônio Dalla Costa
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pré-parto ,bem-estar ,alojamento ,sistema de criação ,farrowing ,welfare ,housing ,husbandry system ,Agriculture (General) ,S1-972 - Abstract
This work compared the behaviour of pre-parturient sows housed in intensive confined and outdoor systems, during the morning and afternoon prior to delivery. Eight sows were kept individually in farrowing crates or in paddocks with access to fresh pasture from 8 to 10 days before expected parturition. All outdoor sows built a nest within 24 hours before farrowing. On the morning and afternoon before farrowing they spent 6.5% of the time collecting grass and 7.5% of the time organizing the nest. Outdoor sows were observed more often than confined sows rooting (4.60% vs. 0.25%), feeding (10.2% vs. 3.0%), standing (51% vs. 13%) and walking (8% vs. 0%). Indoor sows redirected the nesting behaviour to other behaviours like attempts to dig the ground, nosing, biting and rooting parts of the crate, feeder or drinker, during 4.7% of the time. They also spent more time than outdoor sows inactive (85% vs. 60%), lying (72% vs. 41%), drinking (2.1% vs. 0.5%) and vacuum chewing (3.7% vs. 0.1%). The pre-parturient behaviour of sows was considerably affected by the husbandry system. The outdoor system appears to be more appropriate for the sows' welfare than the conventional confinement.Neste trabalho foi comparado o comportamento de porcas pré-parturientes criadas no sistema confinado convencional ou no sistema intensivo ao ar livre. Oito porcas foram mantidas individualmente em baias de maternidade ou em potreiros com acesso a pasto fresco, de oito a dez dias antes da data prevista para o parto. As porcas ao ar livre construíram um ninho nas 24 horas antes do parto. Na manhã e tarde antes do parto, passaram aproximadamente 6,5% do tempo coletando pasto e 7,5% do tempo organizando um ninho. As porcas ao ar livre foram observadas mais freqüentemente do que as confinadas fuçando (4,60% vs. 0,25%), comendo (10,2% vs. 3,0%), em pé (51% vs. 13%) e caminhando (8% vs. 0%). As porcas confinadas redirecionaram o comportamento de ninho para comportamentos como tentativas de escavar no piso, morder e fuçar partes da baia, o comedouro e o bebedouro, durante 4,7% das observações. Também foram observadas mais freqüentemente do que as porcas ao ar livre inativas (85% vs. 60%), deitadas (72% vs. 41%), bebendo (2,1% vs. 0,5%) e mascando em falso (3,7% vs. 0,1%). O comportamento de porcas pré-parturientes foi consideravelmente influenciado pelo sistema de criação. O sistema intensivo ao ar livre parece ser mais apropriado para o bem-estar de porcas nesta fase do que o confinamento convencional.
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- 2005
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26. Characterization of the floristic composition of the pastureland in the cattle area in a farm of Florianopolis--SC, Brazil
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Olivera-Castro, Yuseika, Azevedo, Maiara Mendes de, Arias-Aviles, Laura Livia, Filho, Luiz Carlos Pinheiro Machado, and Pozo-Rodriguez, Pedro Pablo del
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- 2020
27. CRIAÇÃO ANIMAL AGROECOLÓGICA
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Luiz Carlos Pinheiro Machado Filho, José Alfredo Bran Agudelo, Fabiellen Cristina Pereira, Gabriela Schenato Bica, Adenor Vicente Wendling, Daniele Cristina Silva Kazama, and Shirley Kuhnen
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General Medicine - Abstract
Os sistemas agrícolas convencionais têm promovido desmatamento, emissão de gases de efeito estufa, perda da fertilidade de solos, dependência de insumos com consequente aumento de custos, o êxodo e a masculinização do meio rural. A alardeada produtividade tem servido mais para satisfazer os interesses do mercado, do que para atender às necessidades alimentares das pessoas. Os sistemas agrícolas que adotam práticas agroecológicas favorecem a provisão de serviços ecossistêmicos e alimentos de qualidade. A agroecologia é uma ciência multi, inter e transdisciplinar, capaz de sustentar sistemas agrícolas, incluindo a criação animal, reduzindo impactos ambientais, com produção de alimentos de qualidade, e rentabilidade econômica. Essa revisão objetiva contextualizar os desafios para a criação animal agroecológica e suas possibilidades de integração com a produção vegetal para equilibrar diversos benefícios nos âmbitos ambiental, sanitário e econômico.
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- 2023
28. Homeopathic remedies in a semi-intensive alternative system of broiler production
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Rosane Amalcaburio, Luiz Carlos Pinheiro Machado Filho, Luciana Aparecida Honorato, and Nelton Antônio Menezes
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Complementary and alternative medicine - Abstract
This experiment had the objective of evaluating the influence of homeopathic remedies on the performance and health of broilers in a semi-intensive alternative system of production. Two groups of 90 Cobb strain broilers were randomly allocated, at 21 days of life, to three treatments: Control, Calcarea carbonica 12cH and Calcarea phosphorica 12cH, in a randomized block design in two stages. The medication was administered in the birds' drinking water with a 5% hydro-alcohol extract solution in 5 drops per bird daily during 28 days, beginning at 22 days of age. Each stage comprised three blocks, totalizing six replications per treatment. Each treatment group consisted of 10 broilers. Birds were weighed at 21 days and weekly thereon, up to slaughter at 49 days. After slaughtering, carcasses, hearts, livers, gizzards and feet were also weighed. Differences among stages in weight gain (Stage 1 = 2,576 ± 38 g; Stage 2 = 2,825 ± 38 g; P < 0,0004) and in final weight (Stage 1 = 3,470 ± 38 g, Stage 2 = 3,348 ± 40 g; P < 0.03) were noted. There were no differences between stages or treatments for the variables of carcass, feet and gizzard weights (P > 0.40). The liver and heart, however, weighed significantly more in stage 2 (P < 0.01), which may be interpreted as an indicator of greater metabolic activity of these organs coincident with greater weight gain within the same period. When compared to the other two treatments, liver weight proved lower (P < 0.05) in the Calcarea carbonica treatment group, as expected and in accordance to reports of the materia medica of this medicine. In conclusion, there was no effect of homeopathy applied for the purpose of growth. Lower liver weights of the Calcarea carbonica-treated birds may be associated to a lower susceptibility to stress. This possibility, however, calls for new studies for verification. Keywords: Homeopathy, Poultry production, Agroecology.  Medicamentos homeopáticos em um sistema alternativo de produção de frangos. Resumo Este estudo objetivou avaliar a influéncia de medicamentos homeopáticos no desempenho e saúde de frangos, em um sistema alternativo semi-intensivo de produção. Dois grupos de 90 frangos Cobb foram randomicamente distribuídos, aos 21 dias de vida, entre 3 diferentes tratamentos: controle, Calcárea carbonica 12cH y Calcarea phosphorica 12 ch, em 2 fases. A medicação foi administrada usando-se uma solução hidroalcoólica (água potável) 5%, numa razão de 5 gotas por animal, diariamente durante 28 dias, começando aos 22 dias de vida. Cada fase incluiu 3 blocos, totalizando 6 replicações por tratamento, Cada grupo de tratamento continha 10 animais. Os frangos foram pesados aos 21 dias de vida e depois, semanalmente, até o abate, aos 49 dias. Depois do abate, carcaça, coração, fígado, moela e patas foram pesadas. Foram registradas diferenças de aumento de peso entre as diversas fases (Fase 1= 2.576 ± 38 g; Fase 2 = 2.825 ± 38 g; p < 0,0004) e no peso final (Fase 1 = 3.470 ± 38 g, Fase 2 = 3.348 ± 40 g; p < 0.03). Não houve diferença entre as fases e tratamentos para o peso da carcaça, patas e moela (p>0,4). Por outro lado, coração e fígado apresentaram aumento significativo de peso na fase 2 (p < 0,01), que pode ser interpretado como indicador de maior atividade metabólica nestes órgãos, coincidente com o maior aumento de peso no mesmo período. Quando comparados os 2 tratamentos, o peso do fígado foi menor (p < 0,05) no grupo tratado com Calcarea carbonica, conforme esperado e de acordo com a descrição da matéria médica deste medicamento. Concluímos que o tratamento homeopático não teve efeito quando aplicado com finalidade de crescimento. O menor peso do fígado no tratamento com Calcarea carbonica pode estar associado a menor susceptibilidade ao estresse. Essa possibilidade exige novos estudos e protocolos, específicos. Palavras-chave: Homeopatia, Produção avícola, Agroecologia.  Medicamentos homeopáticos en un sistema alternativo semi-intensivo de producción de pollos Resumen Este estudio objetivó evaluar la influencia de medicamentos homeopáticos en la performance y salud de pollos en un sistema alternativo semi-intensivo de producción. Dos grupos de 90 pollos Cobb fueron randomizadamente distribuidos, a los 21 días de vida, entre 3 tratamientos: control; Calcarea carbonica 12cH y Calcarea phosphorica 12cH en 2 fases. La medicación fue administrada en el agua potable en solución hidroalcohólica 5%, a razón de 5 gotas por animal, diariamente durante 28 días, comenzando a los 22 días de vida. Cada fase incluyó 3 bloques, totalizando 6 replicaciones por tratamiento. Cada grupo de tratamiento comprendió 10 animales. Los pollos fueron pesados a los 21 días de vida y después, semanalmente, hasta el abate, a los 49 días. Después del abate, cuerpo, corazón, hígado, molleja y patas fueron pesados. Fueron registradas diferencias de aumento de peso entre las diversas fases (Fase 1 = 2,576 ± 38 g; Fase 2 = 2,825 ± 38 g; p < 0,0004) y en el peso final (Fase 1 = 3,470 ± 38 g, Fase 2 = 3,348 ± 40 g; p < 0.03). No hubo diferencias entre las fases y tratamientos para el peso de cuerpo, patas y molleja (p>0,4). Por otro lado, corazón e hígado presentaron aumento significativo de peso en la fase 2 (p < 0,01), que puede ser interpretado como indicador de una mayor actividad metabólica en estos órganos, coincidente con el mayor aumento de peso en el mismo período. Cuando comparado a los otros 2 tratamientos, el peso del hígado fue menor (p < 0,05) en el grupo tratado con Calcarea carbonica, como esperado y de acuerdo con la descripción en la materia médica de este medicamento. Concluyendo, el tratamiento homeopático no tuvo efecto cuando aplicado con fines de crecimiento; el menor peso del hígado en el tratamiento con Calcarea carbonica puede estar asociado a menor susceptibilidad a stress. Esta posibilidad exige nuevos estudios dedicados a verificarla. Palabras-clave: Homeopatia, Producion de aves, Agroecologia.  Correspondence author: Luiz Carlos Pinheiro Machado Filho, pinheiro@cca.ufsc.br How to cite this article: Amalcaburio R, Machado Filho LCP, Honorato LA, Menezes NA. Homeopathic remedies in a semi-intensive alternative system of broiler productio. Int J High Dilution Res [online]. 2009 [cited YYYY Month dd]; 8 (26): 33-39. Available from: http://journal.giri-society.org/index.php/ijhdr/article/view/325/383. ÂÂÂ
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- 2021
29. Effect of pasture management on bioactive compounds of Lolium multiflorum and Avena strigosa for dairy cows and its effect on milk quality
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Juliana Roemers Moacyr, Isadora Nicole Piccinin, Shirley Kuhnen, Luiz Carlos Pinheiro Machado Filho, and Daline Tais Holz
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chemistry.chemical_classification ,chemistry ,Agronomy ,biology ,Renewable Energy, Sustainability and the Environment ,Avena strigosa ,food and beverages ,Pasture management ,Lolium multiflorum ,Development ,biology.organism_classification ,Agronomy and Crop Science ,Carotenoid - Abstract
This study aimed to evaluate the effects of pasture management on bioactive compounds and its effects on milk quality. The total phenolic content in Avena strigosa and Lolium multiflorum was greate...
- Published
- 2021
30. Microclimate and pasture area preferences by dairy cows under high biodiversity silvopastoral system in Southern Brazil
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Luiz Carlos Pinheiro Machado Filho, Karolini Tenffen de Sousa, Maria José Hötzel, Abdon Luiz Schmitt Filho, Paulo Antônio de Almeida Sinisgalli, and Matheus Deniz
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030203 arthritis & rheumatology ,Atmospheric Science ,geography ,geography.geographical_feature_category ,010504 meteorology & atmospheric sciences ,Ecology ,Health, Toxicology and Mutagenesis ,Bioclimatology ,Microclimate ,Biodiversity ,Humid subtropical climate ,01 natural sciences ,Pasture ,03 medical and health sciences ,0302 clinical medicine ,Animal science ,Air temperature ,Grazing ,Environmental science ,Restoration ecology ,0105 earth and related environmental sciences - Abstract
The aim of this study was to evaluate the influence of microclimate on dairy cows’ behaviors and their preferences for different pasture areas under high biodiversity silvopastoral system (SPSnuclei) in a subtropical climate. We surveyed three different pasture areas under SPSnuclei: shaded area around the nuclei (SAN), unshaded area around the nuclei (UAN), and all-day sunny area distant from the nuclei (SDN). In each area, the microclimatic variables were measured—air temperature (°C), relative humidity (%), illuminance (lux), wind speed (m/s), and soil surface temperature (°C). In addition, the diurnal behaviors of 39 Jersey dairy cows were evaluated. Grazing, standing rest, lying rest, standing rumination, and lying rumination were registered by scans every 10 min; drinking water was observed continuously. Microclimate differed (p
- Published
- 2020
31. Grazing Behaviour and Welfare of Ruminants
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Luiz Carlos Pinheiro Machado Filho and Pablo Gregorini
- Published
- 2022
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32. Development of propolis nanoparticles for the treatment of bovine mastitis: in vitro studies on antimicrobial and cytotoxic activities
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Letícia Mazzarino, Luiz Carlos Pinheiro Machado Filho, Marcelo Maraschin, Maria Beatriz Veleirinho, Ronaldo L.A. Cerri, Gabriela Tasso Pinheiro Machado, and Shirley Kuhnen
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0301 basic medicine ,Traditional medicine ,Chemistry ,Nanoparticle ,02 engineering and technology ,Propolis ,021001 nanoscience & nanotechnology ,medicine.disease ,Antimicrobial ,In vitro ,Mastitis ,03 medical and health sciences ,030104 developmental biology ,Food Animals ,medicine ,Cytotoxic T cell ,heterocyclic compounds ,Animal Science and Zoology ,0210 nano-technology - Abstract
This study describes the development of propolis nanoparticles (PNP) to treat bovine mastitis. Three PNP prepared with varying concentrations of propolis (5% and 7%, w/v) and the surfactants [poloxamer (1%, 3%, and 4%, w/v) and soy lecithin (0.25%, 0.7%, and 1%, w/v)]. PNP were characterized according to their size, polydispersity, zeta potential, pH, morphology, and physical stability. PNP were evaluated for their in vitro antimicrobial and cytotoxic effects. PNP obtained were spherical with a monodisperse distribution (polydispersity index −1. The IC50 (the concentration responsible for reduction of cellular viability by half) for epithelial cells of bovine mammary gland (MAC-T, mammary alveolar cell-T) varied from 122.2 to 268.4 μg mL−1. Results showed that PNP represent a promising nanocarrier for high concentrations of propolis extract in a stable aqueous medium, while, at the same time, presenting antimicrobial activity accompanied by moderate cytotoxicity to the MAC-T cells.
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- 2019
33. Grazing Behaviour and Welfare of Ruminants
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Filho, Luiz Carlos Pinheiro Machado, primary and Gregorini, Pablo, additional
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- 2022
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34. Beef Cattle on Pasture Have Better Performance When Supplied With Water Trough Than Pond
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Luiz Carlos Pinheiro Machado Filho, Dayane Lemos Teixeira, and Gabriela Schenato Bica
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040301 veterinary sciences ,Veterinary medicine ,Water source ,Beef cattle ,Biology ,Trough (economics) ,Pasture ,water intake ,0403 veterinary science ,Animal science ,Grazing ,SF600-1100 ,medicine ,average daily gain ,Water intake ,Original Research ,geography ,geography.geographical_feature_category ,General Veterinary ,behavior ,0402 animal and dairy science ,04 agricultural and veterinary sciences ,040201 dairy & animal science ,extensive pasture ,Walking time ,beef cattle welfare ,Veterinary Science ,medicine.symptom ,Weight gain - Abstract
The behavior and performance of steers on pasture regarding water availability in troughs or in ponds were compared. Eight paddocks were randomly allocated to one treatment: POND (~30 m of diameter) or TROUGH (water trough, 120 cm diameter and 60 cm high and 500 L capacity). Eight groups of six beef steers were randomly assigned to one of the paddocks. The first 10 days were considered for animal habituation. Animals were individually weighed (days 0, 30, 60, and 90). Beginning in the day after each weighing on days 30 (Month 1), 60 (Month 2), and 90 (Month 3), behavior and animal distribution in the paddock were recorded by direct visual observation in three periods of 4 consecutive days. Water temperature and fecal and herbage DM were also recorded in these periods. Water intake was measured during 16 random days in the troughs. Data were analyzed using Generalized Linear Mixed Models, with treatment and period as fixed effects. TROUGH steers gained more weight (0.44 vs. 0.34 kg/day/animal; P ≤ 0.007) during the experiment and were heavier than the others at the end of the study (P ≤ 0.05). POND steers spent more time drinking water, but TROUGH steers increased the number of drinking events throughout the study (P ≤ 0.05), suggesting an adaptation for the new type of water source. Both treatments increased grazing time throughout the study, but not ruminating time (P ≤ 0.05). Walking time differed between treatments in all periods of behavior observation (P ≤ 0.05). Events of animal licking and ingesting salt of POND steers reduced throughout the study (P ≤ 0.05). The number of drinking events of TROUGH steers increased throughout the study, and drinking events were longer for POND steers than TROUGH steers (P ≤ 0.05). TROUGH steers spent more time on pasture on Month 2 (P ≤ 0.05). Period collection did not affect the water intake of TROUGH treatment (P > 0.05). This study demonstrates that water available in troughs rather than ponds for steers on pasture has positive effects on their weight gain and affects cattle behavior.
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- 2021
35. Identification and antimicrobial susceptibility of milk pathogen isolated from dairy production systems
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Priscila Arrigucci Bernardes, Shirley Kuhnen, Isadora Nicole Piccinin, Dario Fernando Milanez de Mello, Juliana Martins, Luiz Carlos Pinheiro Machado Filho, and Luciana Aparecida Honorato
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Veterinary medicine ,medicine.drug_class ,Staphylococcus ,Antibiotics ,Cattle Diseases ,Microbial Sensitivity Tests ,Biology ,Antibiotic resistance ,Food Animals ,Ampicillin ,Drug Resistance, Bacterial ,medicine ,Animals ,Agar diffusion test ,Mastitis, Bovine ,Streptococcus ,Staphylococcal Infections ,medicine.disease ,Mastitis ,Anti-Bacterial Agents ,Penicillin ,Multiple drug resistance ,Dairying ,Milk ,Animal Science and Zoology ,Gentamicin ,Cattle ,Female ,medicine.drug - Abstract
Livestock has been recognized as a reservoir of antibiotic-resistant bacteria. Prevalence of resistance has been associated with herd size and intensification of animal production systems. Brazil is one of the emergent hotspots of bacterial resistance, which is also associated with animal husbandry. This study aimed to evaluate the resistance profile of pathogens that cause subclinical mastitis and the relationship between resistance status at farm level and different production systems. Milk samples from cows diagnosed with subclinical mastitis were collected from farms that adopt different husbandry systems with different production intensities, i.e., agroecological, low input, high input, Free-Stall and Compost-bedded pack barn. Etiological agents were isolated and microbiologically identified, and antibiotic susceptibility testing was conducted, using the disk diffusion method. The main isolated agents were Streptococcus spp. (n = 54, 30.5 %) and coagulase-positive Staphylococcus (CPS) (n = 54; 30.5 %). The recovered isolates displayed high antibiotic resistance against Sulfamethazine (80.2 %), Gentamicin (29.37 %), Penicillin (29.37 %), Oxacillin (28.82 %) and Ampicillin (26 %). Multidrug resistance was found for all agents and in all farming systems (39.54 %). Neither production systems (p = 0.26) nor farming systems (p = 0.24) significantly affected the resistance rates of samples. Therefore, intensive production systems may not be a root cause of increased rates of antimicrobial resistance in the milk production chain, suggesting that other environmental factors should be investigated. It is noteworthy that high levels of multidrug resistance were even found in bacteria earlier considered as minor pathogens. This development can be taken as a warning that environmental bacteria are potential transmitters of resistance genes to the environment.
- Published
- 2021
36. Scratching, cleaning and social bonding: grooming and their biological meaning in ruminants/Cocar-se, limpar-se e estabelecer vinculos sociais: o 'grooming' e seu significado biologico nos ruminantes/Rascarse, limpiarse y establecer vínculos sociales: el comportamiento de grooming y su significado biológico en rumiantes
- Author
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Agudelo, Jose Alfredo Bran, de Quadros, Sergio Augusto Ferreira, and Filho, Luiz Carlos Pinheiro Machado
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- 2013
37. Particularities of the human-animal interactions relevant to the welfare and productivity of dairy cows/Particularidades relevantes da interacao humano-animal para o bem- estar e produtividade de vacas leiteiras
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Honorato, Luciana Aparecida, Hotzel, Maria Jose, Gomes, Carla Christina de Miranda, Silveira, Isabella Dias Barbosa, and Filho, Luiz Carlos Pinheiro Machado
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- 2012
38. INFLUÊNCIA DA HIERARQUIA SOCIAL E DA PROXIMIDADE DO PARTO NO LIMIAR TÉRMICO NOCICEPTIVO EM VACAS PRENHES
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Raphaela E Woodroffe, Karolini Tenffen de Sousa, Luiz Carlos Pinheiro Machado Filho, and João Pedro Donadio da Silva Pereira
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General Veterinary - Abstract
O estabelecimento dos níveis hierárquicos em bovinos se dá pelas relações dedominância, as quais são definidas por interações agonísticas. Vacas subordinadas são as principais vítimas das interações agonísticas e podem desenvolver estresse crônico. O estresse e medo provocado pelas interações agonísticas pode resultar em uma analgesia induzida, que induz a produção de opióides. No período periparturiente também ocorre liberação de opióides para mitigar as dores do parto. Os opióides endógenos, como a endorfina, modulam a transmissão da estimulação da dor nas sinapses neurais em vários pontos das vias do sistema nervoso central, aumentando o limiar nociceptivo. Neste estudo observacional testamos a hipótese de que vacas subordinadas, por serem as principais vítimas das interações agonísticas, estariam em permanente estresse e medo, e apresentariam maior limiar nociceptivo de dor. O experimento foi realizado entre maio e agosto de 2018, na “University of British Columbia - Dairy Education and Research Center” em Agassiz, Canadá. Participaram deste estudo 48 vacas da raça Holandês, entre 21-28 dias de distância do parto, com peso médio de 751 ± 130 kg e alojadas em estabulação livre. As vacas foram mantidas em dois grupos dinâmicos, em baias com densidade de 10 animais cada e o reagrupamento foi realizado semanalmente. Os grupos eram heterogêneos, compostos por animais de diferentes lactações e peso. O limiar nociceptivo de dor foi mensurado semanalmente com um medidor de limiar térmico, no qual um estímulo de calor radiante foi aplicado no tegumento dorsal sobrepondo-se às falanges médias do ante pé do animal, imediatamente acima do casco. Após 60h do reagrupamento foram registradas todas as interações agonísticas que ocorreram na área de alimentação, durante 48h consecutivas. Desta forma, obtivemos a categoria social (dominante, intermediário e subordinado) de cada animal semanalmente até um dia antes do parto. Todos os dados foram analisados com o software Rstudio. Em todo o período experimental foram registrados mais de 2000 eventos de interações agonísticas. Não houve diferença (p=0,833) no número de interações agonísticas à medida que os animais se aproximavam do parto. Houve mudança na categoria social das vacas à medida que se aproximavam do parto, sendo que 25 vacas subiram e/ou desceram de posição na categoria social. Não houve diferença (p>0,05) no limiar nociceptivo de dor entre as categorias sociais (dominantes: 81,73°C; intermediários: 80,18°C; subordinados: 78,86°C). Não houve diferença (p=0.224) no limiar nociceptivo de dor de acordo com a distância do parto. Vacas subordinadas não apresentaram limiar nociceptivo diferente das dominantes no momento final da prenhez, porém a percepção de dor pelas vacas pode ter sido afetada por estarem prenhas. Concluímos que nas condições deste estudo, a hierarquia social e a proximidade do parto não interferiu no limiar nociceptivo de dor de vacas próximas ao parto.
- Published
- 2020
39. RELAÇÃO DA FREQUÊNCIA DE PROXIMIDADE E COMPORTAMENTO DE NOVILHAS LEITEIRAS CRIADAS A PASTO
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Aline Aparecida Cerino, Camila de Almeida Franceschi, Luiz Carlos Pinheiro Machado Filho, Karolini Tenffen de Sousa, Matheus Deniz, and Laura Arias Avilés
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General Veterinary - Abstract
A distribuição espacial dos bovinos na pastagem não ocorre de forma aleatória, sendo que a proximidade com outros animais pode refletir os laços sociais formados dentro de um rebanho. Alguns animais permanecem mais próximos de indivíduos específicos em relação ao restante rebanho, demostrando certa preferência. A frequência de proximidade entre bovinos é dita como interação positiva, pois está presente em organizações sociais complexas e auxilia na manutenção da relação estável entre os animais envolvidos. O objetivo deste estudo observacional foi descrever a frequência de proximidade entre novilhas leiteiras durante seus comportamentos diurnos. O experimento foi realizado entre os meses de outubro e dezembro de 2017, no Biotério de Bovinos da Fazenda Didático-Experimental da Ressacada (FER), pertencente a Universidade Federal de Santa Catarina, Florianópolis, Brasil. Foram observadas 19 novilhas mochas (secas e não-prenhas) mestiças (Jersey x Holandês), com aproximadamente 48 meses de idade e peso médio de 355 ± 40kg. Na FER os animais são manejados sob Pastoreio Racional Voisin, adentrando a um novo piquete (2.500m2) a cada 24h. As observações ocorreram durante 20 dias, alternando os períodos matutino (8:00–12:00) e vespertino (14:00–18:00). Os comportamentos pastando, ócio em pé, ócio deitado, ruminando em pé, ruminando deitado, outros e a proximidade foram avaliados através de instantâneos a cada 10min. Dois animais foram considerados próximos quando estavam a uma distância correspondente ao comprimento do corpo de uma vaca adulta (aproximadamente 2m) da paleta do animal focal. Independente do período (matutino e vespertino), o comportamento pastando apresentou a maior frequência (62,42%) em relação aos demais. Durante o período matutino os animais realizaram preferencialmente os comportamentos de ócio (em pé: 16,6%; deitado: 14,5%) e ruminação (em pé: 4,9%; deitado: 13,3%). As novilhas realizaram a maior frequência dos comportamentos avaliados sozinhas (69%), próximos a um indivíduo (24%) e de dois indivíduos (6,4%). Quando próxima a outra novilha (uma ou duas), o animal focal foi observado com maior frequência pastando (47,8%), seguido de ócio (28,1%), ruminando (19,4%) e realizando outros comportamentos (4,6%). O comportamento de ócio em pé do animal focal foi ocorreu com maior frequência quando próximo de outra novilha (em pé: 19,3%, deitado: 8,8%). Enquanto que para o comportamento de ruminação ocorreu o inverso, as novilhas ruminaram com maior frequência próxima de outra quando estavam deitadas (em pé: 5,7%, deitado: 13,7%). Concluímos, que a atividade de pastoreio foi realizada preferencialmente em companhia de outra novilha, sendo esta uma provável função de coesão social do grupo durante o pastoreio.
- Published
- 2020
40. Incorporating a Fresh Mixed Annual Ryegrass and Berseem Clover Forage Into the Winter Diet of Dairy Cows Resulted in Reduced Milk Yield, but Reduced Nitrogen Excretion and Reduced Methane Yield
- Author
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Luiz Carlos Pinheiro Machado Filho, Deirdre Hennessy, Dayane Lemos Teixeira, Shaun Richard Owen Williams, Daniel Enriquez-Hidalgo, Paula Toro-Mujica, and Fabiellen Cristina Pereira
- Subjects
0106 biological sciences ,Silage ,Forage ,Total mixed ration ,milk quality ,01 natural sciences ,Excretion ,Animal science ,Trifolium alexandrinum ,Lolium multiflorum ,milk production ,Dairy cattle ,Legume ,Original Research ,lcsh:Veterinary medicine ,biology ,General Veterinary ,0402 animal and dairy science ,dairy cattle ,food and beverages ,04 agricultural and veterinary sciences ,biology.organism_classification ,040201 dairy & animal science ,mixed herbage ,total mixed ration ,lcsh:SF600-1100 ,Veterinary Science ,010606 plant biology & botany ,enteric methane - Abstract
The winter diet of dairy cows in Mediterranean climate regions is usually a total mixed ration with a base of conserved summer crops such as corn silage and alfalfa hay. However, there is increased labor and financial cost related to this kind of feeding, which could be reduced if fresh forages were used in place of some of the conserved forage in the cow diet. The objective of our study was to evaluate the effect of including fresh mixed annual ryegrass and berseem clover into the diet of dairy cows on milk, nitrogen utilization, and methane emission. Twenty-four lactating dairy cows were split into two groups and offered either a diet similar to that usually offered to the cows (CON) or one where a mixture of fresh annual ryegrass and berseem clover was used to partially substitute the corn silage and alfalfa hay in the diet (MIX). Milk yield was recorded automatically, and methane emissions were estimated using the SF6 tracer technique. The MIX diet had lower crude protein concentration (148 vs. 170 g/kg DM) but higher DM digestibility (81.6 vs. 78.6%) than the CON diet. Compared to the cows offered the CON diet, milk yield was reduced when cows were fed the MIX diet (36.4 vs. 31.9 kg/d), but methane emissions (381 vs. 332 g/d) and nitrogen excretion were also reduced (238 vs. 180 g/d). Nitrogen use efficiency was unaffected (30.8%). In addition, milk from cows fed the MIX diet had a fatty acid profile considered to be more beneficial to human health than that of the milk from cows fed the CON diet. Increasing the protein concentration in the MIX diet, either by direct supplementation or increasing the proportion of legume in the mixed herbage, could overcome the reduction on milk and positively affect methane emission and N use efficiency.
- Published
- 2020
41. Time of Grain Supplementation and Social Dominance Modify Feeding Behavior of Heifers in Rotational Grazing Systems
- Author
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Dayane Lemos Teixeira, Luiz Carlos Pinheiro Machado Filho, Maria José Hötzel, Karolini Tenffen de Sousa, and Gabriela Schenato Bica
- Subjects
040301 veterinary sciences ,animal diseases ,social hierarchy ,Biology ,Pasture ,0403 veterinary science ,03 medical and health sciences ,Animal science ,Feeding behavior ,agonistic interactions ,Grazing ,Agonistic behaviour ,subordinate ,030304 developmental biology ,Original Research ,0303 health sciences ,geography ,lcsh:Veterinary medicine ,geography.geographical_feature_category ,General Veterinary ,Entry time ,04 agricultural and veterinary sciences ,Breed ,Dominance (ethology) ,cattle ,Social hierarchy ,lcsh:SF600-1100 ,Veterinary Science ,resources - Abstract
Social hierarchy affects the access of animals to feed resources. On daily rotational pasture systems, supplementation time may influence feeding behavior. This trial was designed to test the effect of grain delivery time on the feeding behavior of heifers. Heifers divided into two groups according to breed (n = 15 Braford and n = 19 Jersey) were tested in a crossover design with two treatments: INITIAL—supplement at 8 am (entry time to a fresh paddock), and MIDDLE—supplement at 4 pm (middle time of paddock use). Animals entered a new paddock every morning, and grain supplement at 2 kg/animal/day was offered at the fence line (1 m/animal). Then, ingestive and other behaviors were registered by direct visual observation through scan sampling at 2-min intervals for 1 h after grain supply. Agonistic interactions were recorded continuously (instigator–victim) to build a social matrix whereby each heifer was defined as dominant, intermediate, or subordinate. Weekly pasture samples were collected according to the order that animals left the feeding area, using the hand-plucking technique, to determine crude protein and fiber content. Heifers spent more time grazing on the INITIAL treatment (p < 0.0001) but exhibited more behaviors on the MIDDLE treatment (p < 0.0001). Dominant heifers spent more time eating grain (p = 0.0008), whereas subordinate heifers spent more time grazing along the paddock (p = 0.0067), but not along the fence (p = 0.0008). The crude protein content of pasture samples was higher for the INITIAL treatment (p < 0.0001). Behavioral interaction occurred with respect to the order of leaving the feeding area, social rank, and crude protein consumed (p = 0.04). Subordinate heifers consistently grazed more and ate less grain supplement than dominant and intermediate heifers. However, when grain supplement was offered at the time animals entered the paddock, more grazing activity took place during supplement feeding, and subordinate heifers could select a high-protein diet. In the INITIAL treatment, this means that subordinate animals could benefit from the better pasture available, keeping a distance from dominant heifers, reducing agonistic interactions and likely improving their welfare.
- Published
- 2020
- Full Text
- View/download PDF
42. Social hierarchy influences dairy cows’ use of shade in a silvopastoral system under intensive rotational grazing
- Author
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Matheus Fernando Moro, Maria José Hötzel, Karolini Tenffen de Sousa, Luiz Carlos Pinheiro Machado Filho, Marcos Martinez do Vale, Matheus Deniz, and João Ricardo Dittrich
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Food Animals ,Air temperature ,Grazing ,Humid subtropical climate ,Social hierarchy ,Environmental science ,Thermal comfort ,Animal Science and Zoology ,Relative humidity ,Soil surface temperature ,Atmospheric sciences ,Wind speed - Abstract
The aim of this study was to evaluate the relationship between thermal comfort indicators and social hierarchy, and cows' location (shade or sun) and their diurnal behaviors in a silvopastoral system of a subtropical climate, covering the four seasons. We measured microclimatic variables (air temperature, relative humidity, wind speed, soil surface temperature) and cows’ behaviors in two areas (shaded and sunny), as well as the influence of social hierarchy (dominant, intermediate, and subordinate) on cows' location (shade or sun). In addition, we determined the black globe-humidity index (BGHI) and radiant heat load (RHL) for both areas. Air temperature, wind speed, and soil surface temperature were lower in shaded areas, and relative humidity lower in the sunny areas (p
- Published
- 2021
43. Effects of instantaneous stocking rate, paddock shape and fence with electric shock on dairy cows' behaviour
- Author
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Luiz Carlos Pinheiro Machado Filho, Dayane Lemos Teixeira, Daniel Enriquez-Hidalgo, and Maria José Hötzel
- Subjects
0301 basic medicine ,Fence (finance) ,Stocking rate ,General Veterinary ,Electric shock ,0402 animal and dairy science ,Experimental Unit ,04 agricultural and veterinary sciences ,medicine.disease ,040201 dairy & animal science ,Shock (mechanics) ,03 medical and health sciences ,030104 developmental biology ,Animal science ,Stocking ,Latin square ,Grazing ,medicine ,Animal Science and Zoology ,Mathematics - Abstract
We assessed the effect of high instantaneous stocking rate, paddock shape and fence with electric shock on dairy cows' behaviour under rotational grazing system. Twelve Holstein Friesian lactating cows were used in two consecutive experiments. Experiment 1 used a 4×4 Latin square design with 4 two-day periods and 4 treatments: 2 paddock shapes (rectangular and square) and 2 instantaneous stocking rates (500 and 200 cows/ha). For Experiment 2, cows were divided in 4 groups of 3 cows and submitted to two treatments – smooth wire fence with electric shock (4000 V) and smooth wire fence without electric shock – in a cross-over experimental design with two replicates. Data were analysed in PROC GLIMMIX of SAS. The models included treatments as fixed effects and the group as the experimental unit. Cows in higher instantaneous stocking rate performed less grazing behaviour ( P ≤0.05). This finding was expected, as larger area per animal increase the herbage allowance for grazing, and thus grazing time. These same animals also performed more aggressions, but less idling behaviours ( P ≤0.05). Cows in paddocks without electric shock performed almost 15% more grazing behaviour than cows in paddocks with electric shock ( P ≤0.05), which indicates that they may have recognized the absence of shock, hence not avoiding being close to the fence. Paddock shape did not affect any of the behaviours analysed ( P >0.05). The results from this study reinforce the importance of paddock characteristics on dairy cows' behaviours.
- Published
- 2017
44. Designing Better Water Troughs: Does Trough Color Influence Dairy Cows’ Preference?
- Author
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José Daniel Cazale, Maria José Hötzel, Luiz Carlos Pinheiro Machado Filho, Daniel Enriquez-Hidalgo, and Dayane Lemos Teixeira
- Subjects
0301 basic medicine ,Color ,Biology ,Trough (economics) ,Milking ,03 medical and health sciences ,Animal science ,Grazing ,Animals ,Lactation ,Animal Husbandry ,Dairy cattle ,Behavior, Animal ,General Veterinary ,Drinking Water ,0402 animal and dairy science ,Feeding Behavior ,04 agricultural and veterinary sciences ,Animal Feed ,040201 dairy & animal science ,Dairying ,030104 developmental biology ,Linear Models ,Herd ,Cattle ,Female ,Animal Science and Zoology ,Brazil - Abstract
Eighteen lactating dairy cows were used to elucidate their preference for green, grey, or red troughs. The herd was managed under a rotational grazing system with ad-libitum access to water until 11:30 h. For 9 days, all cows were tested individually following the afternoon milking. Cows drank similar quantities, spent a similar amount of time drinking, and took a similar number of sips from the 3 trough colors (p > .05). In 75% of the tests, cows drank more than 95% of the test period from the same trough. Within this time, the percentage of choices did not differ among colors (33.3% green, 39.0% grey, and 27.7% red). When they chose the red trough, cows spent less time drinking (p ≤ .05) and tended to take fewer sips (p = .07), which could suggest a partial aversion to this color. Suboptimal water trough design may have long-term negative effects on both the production and welfare of dairy cattle; however, the results suggest that color does not play a major role in the drinking behavior of dairy cows.
- Published
- 2017
45. FEIJÃO-DE-PORCO ESTIMULA MAIOR PRODUTIVIDADE E EFICIÊNCIA DE USO DE NITROGÊNIO EM PASTAGEM DO QUE A CROTALARIA
- Author
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Luiz Carlos Pinheiro Machado Filho, Yuseika Oliveira Castro, Pedro Pablo Del Pozo, Adenor Vicente Wendling, and Ilyas Siddique
- Subjects
General Medicine - Abstract
O efeito da consorciaA§A£o de Canavalia ensiformis ou Crotalaria juncea com pastagens de Cynodon nlemfuensis e Panicum maximum, foi comparado A testemunha sem leguminosas. A C. ensiformis proporcionou maior produtividade e a C. juncea maior concentraA§A£o de N na fitomassa das gramAneas, indicando maior eficiAancia de uso de N que a testemunha. A densidade da macrofauna do solo diminuiu ao longo do experimento nos trAas tratamentos, provavelmente devido ao aumento do dA©ficit hAdrico.
- Published
- 2019
46. Degree of affinity among dairy heifers affects access to feed supplementation
- Author
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Matheus Deniz, Gabriela Schenato Bica, Karolini Tenffen de Sousa, Luiz Carlos Pinheiro Machado Filho, and Maria José Hötzel
- Subjects
05 social sciences ,0402 animal and dairy science ,04 agricultural and veterinary sciences ,Biology ,040201 dairy & animal science ,Two stages ,Degree (temperature) ,Animal science ,Dominance (ethology) ,Food Animals ,Agonistic behaviour ,Herd ,0501 psychology and cognitive sciences ,Animal Science and Zoology ,050102 behavioral science & comparative psychology ,Licking - Abstract
The aim of this study was to evaluate the influence of social position and degree of affinity among heifers attempting to access limited feed supplement. This study was divided into two stages: (1) observation of behaviour at the paddock and (2) arena tests. In stage 1, we measured socio-positive interactions (licking and frequency of proximity among animals) and agonistic interactions in a herd of nineteen dairy heifers. The frequency of proximity between animals of different dominance ranks was used as a measure of the degree of affinity. This measure was considered for two arena tests. In the first we compared pairs with high (PHda, n = 7) and low (PLda, n = 7) degree of affinity. In the second arena test a third neutral middle-ranking animal was included, and we compared triads with a pair with high (THda, n = 7) or low (TLda, n = 7) degree of affinity. In the arena tests, feed supplementation was offered to pairs and triads, and latency to access food, time spent at the feeder, and agonistic interactions were measured. The degree of affinity influenced (p
- Published
- 2021
47. Characterization of silage production and the use by dairy farmers in the West of Santa Catarina state
- Author
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Luiz Carlos Pinheiro Machado Filho and Adenor Vicente Wendling
- Subjects
Densidade de silagem ,040301 veterinary sciences ,Silage ,Biology ,engineering.material ,Milking ,0403 veterinary science ,Crop ,Animal science ,Yield (wine) ,Dry matter ,lcsh:Agriculture (General) ,Dairy farming ,Maize cultivation ,Agrotóxico ,Cultivo de milho ,0402 animal and dairy science ,Silage density ,04 agricultural and veterinary sciences ,Crop rotation ,040201 dairy & animal science ,lcsh:S1-972 ,Pesticide ,Milk ,engineering ,Fertilizer ,General Agricultural and Biological Sciences ,Leite - Abstract
Silage has been widely used in dairy production. However, little is known about its use at production level. The goal of this study was to characterize production and usage of silage for the dairy farming in Western Santa Catarina state. Thirty farms were selected randomly by their geographical location, in 11 municipalities of the West of Santa Catarina state, and they were invited to participate in the research. Out of these 30 farms, 6 did not have any dairy activity, and 2 preferred not to participate in the research, thus were not included. Semi-structured interviews and on-site visits were then performed. All data were analyzed with the software R version 3.2.2. 95% of the farmers produced and fed silage to animals. Out of the 22 farmers participating in the study, 82% provided silage for dairy cows during the whole year; in 86% of these farms, the silage was fed right after milking, in an average amount of 6.06 ± 0.63 kg DM (dry matter) AU-1 (animal unit, 500 kg) day-1. The average area used for maize production was 8.46 ha, representing 59% of the area destined to dairy farming. The average silage production was 8.8 and 7.6 t ha-1 year-1 in the season and off-season crop respectively, without significant differences. In most silage production areas, the no-tillage system is adopted, with the use of pesticides, chemical fertilization and transgenic seeds, without soil covering or crop rotation. Farms that fertilized maize based on soil analysis had the greatest silage yield, compared to the ones that fertilized without relying on soil analysis. The use of different types of pesticides and fertilizer quantities was not correlated to the silage production volume. Overall the production systems and the quantity of silage produced and fed to the cows do not follow technical criteria; thus silage yield and use could be improved if following strategies to better allocate crop inputs. A silagem tem sido amplamente utilizada na produção leiteira. Entretanto, há pouca informação sobre como é utilizada e produzida. O objetivo desse estudo foi caracterizar os sistemas de produção e uso de silagem para a atividade leiteira no Oeste Catarinense. Foram selecionados 30 estabelecimentos às cegas em mapa digital, em 11 municípios do Extremo Oeste Catarinense, e convidados para participar da pesquisa. Desses 30 estabelecimentos, 6 não possuíam atividade leiteira e 2 preferiram não participar da pesquisa. Foram então realizadas entrevistas semi estruturadas e visitas in loco em 22 estabelecimentos. Os dados foram analisados com o software R versão 3.2.2. 95% dos estabelecimentos produzem e fornecem silagem aos animais. Em 82% das propriedades a silagem é fornecida às vacas leiteiras durante todo o ano, e em 86% destes o fornecimento é realizado logo após as ordenhas, numa quantidade média de 6.06 ± 0.63 (Erro Padrão) kg de MS (matéria seca) UGM-1 (500 kg) dia-1. A área média utilizada para a produção de silagem é de 8,46 ha-1, representando 59% da área destinada para a atividade leiteira. A produção média de silagem é de 8,8 e 7,6 t ha-1 ano-1 na safra e safrinha respectivamente, sem diferença significativa. Na maioria das áreas de produção de silagem adota-se o plantio direto, com uso de agrotóxicos, adubação química e sementes transgênicas, sem cobertura do solo ou rotação de culturas. Nos 5 estabelecimentos que adubaram o milho com base na análise de solo foi alcançado maior produção de silagem, se comparada aos que adubaram sem basear-se na análise do solo. O uso de diferentes tipos de agrotóxicos e quantidade de adubação não apresentaram correlação com o volume de produção de silagem. Em geral, a produção e a quantidade de silagem fornecida aos animais não seguem critérios técnicos, de modo que o rendimento e o uso poderiam ser melhorados através de melhor alocação dos insumos na lavoura.
- Published
- 2018
48. Social hierarchy and feed supplementation of heifers: Line or piles?
- Author
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Luiz Carlos Pinheiro Machado Filho, Dayane Lemos Teixeira, Maria José Hötzel, and Gabriela Schenato Bica
- Subjects
geography ,geography.geographical_feature_category ,05 social sciences ,0402 animal and dairy science ,04 agricultural and veterinary sciences ,Biology ,Body weight ,040201 dairy & animal science ,Pasture ,Dominance (ethology) ,Animal science ,Food Animals ,Corn meal ,Grazing ,Social hierarchy ,Agonistic behaviour ,0501 psychology and cognitive sciences ,Animal Science and Zoology ,050102 behavioral science & comparative psychology - Abstract
Cattle on pasture may have unequal access to grain supplement due to the effect of social dominance. Subordinate animals are known to have less access to resources when competition exists. This trial was designed to test if distributing feed supplement (corn meal) along and under the fence in individual piles would affect heifers feeding behaviour and grant better access to all animals compared to supplement offered in a continuous line. Four groups of nine heifers were used in a 2 × 2 cross-over design, and tested in two treatments: LINE (1 linear meter/animal) and PILE (one pile/heifer distant 1 m from each other). Each period had 3 days for habituation followed by 7 days for data collection. Animals were managed under Voisin’s rotational grazing system and observed for one hour from the moment they entered the new paddock (8:00 to 9:00). Individual behaviour of the heifers / grazing, eating grain supplement, competing or other) was registered at one-minute interval instantaneous scans. All agonistic interactions were recorded and a sociometric matrix was calculated for each group to define each heifer as dominant, intermediate or subordinate. Feeding behaviour as not affected by treatment, but it affected by social hierarchy status. High and intermediate ranking heifers spent more time eating supplement than low ranking ones (P ≤ 0.03). Subordinate heifers grazed longer than intermediate heifers, which in turn grazed longer than dominant heifers (P ≤ 0.01). Social rank did not affect competitive behaviour, but there was a trend for more competition events when concentrate was distributed in piles (P = 0.09). Dominance score was associated with initial body weight (r= 0.686; P ≤ 0.0001). In summary, distributing the grain supplement in individual piles did not benefit the subordinate heifers, as they had less access to the supplement than the dominant ones. When entering a new paddock with supplement offered, the subordinate heifers seemed to avoid competing for grain and spent more time grazing while the dominant heifers spent more time eating supplement.
- Published
- 2019
49. Avaliação do Ciclo de Vida na bovinocultura leiteira e as oportunidades ao Brasil
- Author
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Clandio Favarini Ruviaro, Luiz Carlos Pinheiro Machado Filho, Hizumi Lua Sarti Seó, and Cristiane Maria de Léis
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Land use ,emissões ,0402 animal and dairy science ,Environmental engineering ,Climate change ,04 agricultural and veterinary sciences ,010501 environmental sciences ,Carbon sequestration ,040201 dairy & animal science ,01 natural sciences ,lcsh:TD1-1066 ,lcsh:Environmental engineering ,Agricultural science ,leite ,Production (economics) ,Environmental science ,impactos ambientais ,Environmental impact assessment ,lcsh:Environmental technology. Sanitary engineering ,lcsh:TA170-171 ,Waste Management and Disposal ,Productivity ,Life-cycle assessment ,Dairy cattle ,0105 earth and related environmental sciences - Abstract
RESUMO: A Avaliação do Ciclo de Vida (ACV) é uma ferramenta capaz de estimar o potencial de emissões e apontar as fases críticas de um determinado produto ou processo em todo o seu ciclo de vida. O objetivo deste trabalho foi resumir os principais resultados das pesquisas e estudos de caso em ACV relacionados à bovinocultura leiteira nacional e internacional, de 2008 a 2014, e realizar uma análise crítica das categorias de impacto abordadas com maior frequência pela literatura: mudança climática, acidificação, eutrofização, uso da terra e demanda de energia. De todas as fases, a produção de leite na fazenda é onde ocorre a maior parte das emissões. Os resultados apontam que os pontos críticos da bovinocultura leiteira são as emissões entéricas, produção e uso de fertilizante, uso de esterco, produção e transporte de concentrado, e a baixa produtividade animal. Em geral, a categoria de mudança climática foi contraditória com as demais categorias, razão pela qual não deve ser considerada sozinha em avaliações de impacto ambiental. No Brasil, a intensificação na produção à base de pasto apresenta-se como uma estratégia de redução de impactos, uma vez que diminui a necessidade do uso de insumos de base não renovável e aumenta o sequestro de carbono via fotossíntese.
- Published
- 2017
50. Phenolic content and ferric reducing-antioxidant power of cow's milk produced in different pasture-based production systems in southern Brazil
- Author
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Bruno Bachiega Navarro, Luiz Carlos Pinheiro Machado Filho, Luciana Aparecida Honorato, Jaqueline K Mayer, Rafael Trevisan, Marcelo Maraschin, Juliana Roemers Moacyr, and Shirley Kuhnen
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Nutrition and Dietetics ,Antioxidant ,medicine.medical_treatment ,Forage ,Biology ,Antioxidant capacity ,Uv spectra ,Animal science ,Agronomy ,parasitic diseases ,Grazing ,medicine ,Ferric ,Composition (visual arts) ,Pasture based ,Agronomy and Crop Science ,Food Science ,Biotechnology ,medicine.drug - Abstract
BACKGROUND Phenolic compounds are widely present in forage. However, few studies have been carried out to investigate the presence of these compounds in animal products such as milk. In this study, the total phenolic content (TPC) and ferric reducing–antioxidant power (FRAP) of milk produced under agroecological and conventional management systems in southern Brazil were determined. Three systems were compared: (a) agroecological, with intensive rotational grazing, also known as Voisin grazing (ECO); (b) semi-intensive conventional (SIC); and (c) conventional grazing (CGR) (n = 8 per group). Pastures with distinct and more diverse botanical composition were observed on the ECO farms. RESULTS Significantly (P < 0.05) lower amounts of TPC were found in agroecologically produced milk in autumn and summer and the CGR system showed the highest FRAP value (P < 0.05) for the samples collected in autumn. Positive correlations where found between the TPC and FRAP values obtained for the milk samples (0.198, P < 0.05), milk FRAP and forage TPC values (0.344, P < 0.05), and the TPC and FRAP values obtained for the forage (0.70, p < 0.01). PCA applied to the UV spectra dataset (200–350 nm) clearly distinguished the samples collected from the ECO system in the winter. CONCLUSION Our results revealed that, under the conditions prevalent in southern Brazil, since the cows were allowed to graze in all of the systems, the TPC and antioxidant capacity of the milk samples showed minimal variation. However, since the forage TPC and FRAP values for the milk were correlated, TPC appears to be a promising variable for the purpose of monitoring forage prior to its selection aimed at enhancing the antioxidant activity of milk. © 2014 Society of Chemical Industry
- Published
- 2014
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