4 results on '"Luis BS"'
Search Results
2. Resistin expression in human monocytes is controlled by two linked promoter SNPs mediating NFKB p50/p50 binding and C-methylation.
- Author
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Kumar D, Lee B, Puan KJ, Lee W, Luis BS, Yusof N, Andiappan AK, Del Rosario R, Poschmann J, Kumar P, DeLibero G, Singhal A, Prabhakar S, De Yun W, Poidinger M, and Rötzschke O
- Subjects
- Cells, Cultured, DNA Methylation, Epigenesis, Genetic, Humans, Promoter Regions, Genetic, Protein Binding, Protein Multimerization, Monocytes metabolism, NF-kappa B p50 Subunit metabolism, Polymorphism, Single Nucleotide, Resistin genetics
- Abstract
Resistin is a key cytokine associated with metabolic and inflammatory diseases. Especially in East Asian populations, the expression levels are strongly influenced by genetic polymorphisms. Mechanisms and functional implications of this genetic control are still unknown. By employing reporter assays, EMSA, inhibition studies, bisulphite sequencing, ChIP-Seq and gene-editing we show that the p50/p50 homodimer known to act as repressor for a number of pro-inflammatory genes plays a central role in the genetic regulation of resistin in monocytes along with promoter methylation. In the common RETN haplotype p50/p50 constitutively dampens the expression by binding to the promoter. In an Asian haplotype variant however this interaction is disrupted by the A allele of rs3219175. The SNP is in very close linkage to rs34861192, a CpG SNP, located 280 bp upstream which provides an allele-specific C-methylation site. rs34861192 is located in a 100 bp region found to be methylated in the common but not in the Asian haplotype, resulting in the latter having a higher basal expression, which also associates with elevated histone acetylation (H3K27ac). Genotype associations within cohort data of 200 East Asian individuals revealed significant associations between this haplotype and the plasma levels of factors such as TGF-b, S100B, sRAGE and IL-8 as well as with myeloid DC counts. Thus, the common RETN haplotype is tightly regulated by the epigenetic mechanism linked to p50/p50-binding. This control is lost in the Asian haplotype, which may have evolved to balance the antagonistic RETN effects on pathogen protection vs. metabolic and inflammatory disease induction.
- Published
- 2019
- Full Text
- View/download PDF
3. Insights into the suppressor of T-cell receptor (TCR) signaling-1 (Sts-1)-mediated regulation of TCR signaling through the use of novel substrate-trapping Sts-1 phosphatase variants.
- Author
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Luis BS and Carpino N
- Subjects
- Amino Acid Substitution, Animals, Catalytic Domain, Cell Line, Cells, Cultured, Humans, Mice, Mice, Knockout, Mutant Proteins antagonists & inhibitors, Mutant Proteins chemistry, Mutant Proteins metabolism, Peptide Fragments antagonists & inhibitors, Peptide Fragments chemistry, Peptide Fragments genetics, Peptide Fragments metabolism, Phosphorylation, Protein Kinase Inhibitors pharmacology, Protein Processing, Post-Translational, Protein Tyrosine Phosphatases antagonists & inhibitors, Protein Tyrosine Phosphatases chemistry, Protein Tyrosine Phosphatases genetics, Receptors, Antigen, T-Cell antagonists & inhibitors, Recombinant Proteins chemistry, Recombinant Proteins metabolism, Specific Pathogen-Free Organisms, Substrate Specificity, T-Lymphocytes cytology, T-Lymphocytes drug effects, T-Lymphocytes metabolism, ZAP-70 Protein-Tyrosine Kinase antagonists & inhibitors, ZAP-70 Protein-Tyrosine Kinase genetics, Lymphocyte Activation drug effects, Protein Tyrosine Phosphatases metabolism, Receptors, Antigen, T-Cell metabolism, Signal Transduction drug effects, T-Lymphocytes enzymology, ZAP-70 Protein-Tyrosine Kinase metabolism
- Abstract
High affinity substrate-trapping protein tyrosine phosphatases have been widely used both to investigate the endogenous targets of many phosphatases and to address questions of substrate specificity. Herein, we extend the concept of a substrate-trapping phosphatase to include an enzyme of the histidine phosphatase superfamily. This is the first description of substrate-trapping technology applied to a member of the histidine phosphatase family. The phosphatase suppressor of T-cell receptor signaling (Sts)-1 has recently been reported to negatively regulate signaling downstream of the T-cell receptor. We generated high-affinity substrate-trapping variants of Sts-1 by mutagenesis of key active site residues within the phosphatase catalytic domain. Mutation of both the nucleophilic His380 and the general acid Glu490 yielded Sts-1 enzymes that were catalytically inactive but showed high affinity for an important tyrosine kinase in T cells that Sts-1 is known to regulate, Zap-70. Sts-1 substrate-trapping mutants isolated tyrosine-phosphorylated Zap-70 from lysates of activated T cells, validating Zap-70 as a possible substrate for Sts-1 and highlighting the efficacy of the mutants as substrate-trapping agents. Inhibition of the Zap-70 interaction by vanadate suggests that the substrate-trapping effect occurred via the Sts-1 phosphatase active site. Finally, overexpression of Sts-1 substrate-trapping mutants in T cells blocked T-cell receptor signaling, confirming the inhibitory effect of Sts-1 on Zap-70., (© 2013 FEBS.)
- Published
- 2014
- Full Text
- View/download PDF
4. Computationally driven, quantitative experiments discover genes required for mitochondrial biogenesis.
- Author
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Hess DC, Myers CL, Huttenhower C, Hibbs MA, Hayes AP, Paw J, Clore JJ, Mendoza RM, Luis BS, Nislow C, Giaever G, Costanzo M, Troyanskaya OG, and Caudy AA
- Subjects
- Cell Respiration genetics, Cytoplasm chemistry, Genes, Mitochondrial, Mitochondrial Proteins, Mutant Proteins, Mutation, Proteins genetics, Proteomics, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae growth & development, Mitochondria genetics, Proteins physiology, Saccharomyces cerevisiae ultrastructure
- Abstract
Mitochondria are central to many cellular processes including respiration, ion homeostasis, and apoptosis. Using computational predictions combined with traditional quantitative experiments, we have identified 100 proteins whose deficiency alters mitochondrial biogenesis and inheritance in Saccharomyces cerevisiae. In addition, we used computational predictions to perform targeted double-mutant analysis detecting another nine genes with synthetic defects in mitochondrial biogenesis. This represents an increase of about 25% over previously known participants. Nearly half of these newly characterized proteins are conserved in mammals, including several orthologs known to be involved in human disease. Mutations in many of these genes demonstrate statistically significant mitochondrial transmission phenotypes more subtle than could be detected by traditional genetic screens or high-throughput techniques, and 47 have not been previously localized to mitochondria. We further characterized a subset of these genes using growth profiling and dual immunofluorescence, which identified genes specifically required for aerobic respiration and an uncharacterized cytoplasmic protein required for normal mitochondrial motility. Our results demonstrate that by leveraging computational analysis to direct quantitative experimental assays, we have characterized mutants with subtle mitochondrial defects whose phenotypes were undetected by high-throughput methods., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2009
- Full Text
- View/download PDF
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