396 results on '"Luis A. Barrera"'
Search Results
2. Use of a neuron-glia genome-scale metabolic reconstruction to model the metabolic consequences of the Arylsulphatase a deficiency through a systems biology approach
- Author
-
Olga Y. Echeverri-Peña, Diego A. Salazar-Barreto, Alexander Rodríguez-Lopez, Janneth González, Carlos J. Alméciga-Díaz, Cristian H. Verano-Guevara, and Luis A. Barrera
- Subjects
Metachromatic leukodystrophy ,System biology ,Arylsulphatase A ,Myelin sheath ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Metachromatic leukodystrophy (MLD) is a human neurodegenerative disorder characterized by progressive damage on the myelin band in the nervous system. MLD is caused by the impaired function of the lysosomal enzyme Arylsulphatase A (ARSA). The physiopathology mechanisms and the biochemical consequences in the brain of ARSA deficiency are not entirely understood. In recent years, the use of genome-scale metabolic (GEM) models has been explored as a tool for the study of the biochemical alterations in MLD. Previously, we modeled the metabolic consequences of different lysosomal storage diseases using single GEMs. In the case of MLD, using a glia GEM, we previously predicted that the metabolism of glycosphingolipids and neurotransmitters was altered. The results also suggested that mitochondrial metabolism and amino acid transport were the main reactions affected. In this study, we extended the modeling of the metabolic consequences of ARSA deficiency through the integration of neuron and glial cell metabolic models. Cell-specific models were generated from Recon2, and these were used to create a neuron-glial bi-cellular model. We propose a workflow for the integration of this type of model and its subsequent study. The results predicted the impairment pathways involved in the transport of amino acids, lipids metabolism, and catabolism of purines and pyrimidines. The use of this neuron-glial GEM metabolic reconstruction allowed to improve the prediction capacity of the metabolic consequences of ARSA deficiency, which might pave the way for the modeling of the biochemical alterations of other inborn errors of metabolism with central nervous system involvement.
- Published
- 2021
- Full Text
- View/download PDF
3. Phylogenetics of the genus Sechium P. Brown: A review
- Author
-
Luis A. Barrera-Guzmán, Jorge Cadena-Iñiguez, Juan P. Legaria-Solano, and Jaime Sahagún-Castellanos
- Subjects
genetic flow ,hybridizations ,genetic diversity ,ecological niches ,conservation ,breeding ,Agriculture - Abstract
The Sechium P. Br. genus composed of 11 species, which originated from the mountainous regions of Mesoamerica, have been domesticated and diversified. These species are clustered in two large groups: the Mexican clade and the Central American clade. Morphological and molecular studies have shown that species of the Mexican clade are formed through interspecific hybridizations and genetic flow, with the exception of S. mexicanum, which is strongly linked to the genus Sicyos. The objective of this review was to analyze the phylogenetics of Sechium based on morphological and molecular studies, which contributed to taxonomic knowledge and utilization, thereby favoring its conservation and improvement. The Central American clade is well supported with molecular data, but not so with morphological data. The species in this clade were geographically isolated and endemic. S. edule and S. tacaco are exploited species in the agricultural and industrial sectors, and both have an extensive genetic and phenotypic diversity that has allowed them to diversify and expand into different ecological niches. Finally, the Central American species of Sechium thrive in adverse environments of temperatures of mesophyll forest and high relative humidity, with characteristics that can give resistance to frosts and phytopathogenic agents, as well as cultivated species of this genus.
- Published
- 2021
- Full Text
- View/download PDF
4. Neural stem cells for disease modeling and evaluation of therapeutics for Tay-Sachs disease
- Author
-
Mylinh Vu, Rong Li, Amanda Baskfield, Billy Lu, Atena Farkhondeh, Kirill Gorshkov, Omid Motabar, Jeanette Beers, Guokai Chen, Jizhong Zou, Angela J. Espejo-Mojica, Alexander Rodríguez-López, Carlos J. Alméciga-Díaz, Luis A. Barrera, Xuntian Jiang, Daniel S. Ory, Juan J. Marugan, and Wei Zheng
- Subjects
Tay-Sachs disease ,Induced pluripotent stem cells ,Neural stem cells ,Cyclodextrin ,HPβCD ,δ-tocopherol ,Medicine - Abstract
Abstract Background Tay-Sachs disease (TSD) is a rare neurodegenerative disorder caused by autosomal recessive mutations in the HEXA gene on chromosome 15 that encodes β-hexosaminidase. Deficiency in HEXA results in accumulation of GM2 ganglioside, a glycosphingolipid, in lysosomes. Currently, there is no effective treatment for TSD. Results We generated induced pluripotent stem cells (iPSCs) from two TSD patient dermal fibroblast lines and further differentiated them into neural stem cells (NSCs). The TSD neural stem cells exhibited a disease phenotype of lysosomal lipid accumulation. The Tay-Sachs disease NSCs were then used to evaluate the therapeutic effects of enzyme replacement therapy (ERT) with recombinant human Hex A protein and two small molecular compounds: hydroxypropyl-β-cyclodextrin (HPβCD) and δ-tocopherol. Using this disease model, we observed reduction of lipid accumulation by employing enzyme replacement therapy as well as by the use of HPβCD and δ-tocopherol. Conclusion Our results demonstrate that the Tay-Sachs disease NSCs possess the characteristic phenotype to serve as a cell-based disease model for study of the disease pathogenesis and evaluation of drug efficacy. The enzyme replacement therapy with recombinant Hex A protein and two small molecules (cyclodextrin and tocopherol) significantly ameliorated lipid accumulation in the Tay-Sachs disease cell model.
- Published
- 2018
- Full Text
- View/download PDF
5. Research, diagnosis and education in inborn errors of metabolism in Colombia: 20 years’ experience from a reference center
- Author
-
Olga Y. Echeverri, Johana M. Guevara, Ángela J. Espejo-Mojica, Andrea Ardila, Ninna Pulido, Magda Reyes, Alexander Rodriguez-Lopez, Carlos J. Alméciga-Díaz, and Luis A. Barrera
- Subjects
Inborn errors of metabolism ,Colombia ,Latin America ,Research ,Education ,Diagnosis ,Medicine - Abstract
Abstract The use of specialized centers has been the main alternative for an appropriate diagnosis, management and follow up of patients affected by inborn errors of metabolism (IEM). These centers facilitate the training of different professionals, as well as the research at basic, translational and clinical levels. Nevertheless, few reports have described the experience of these centers and their local and/or global impact in the study of IEM. In this paper, we describe the experience of a Colombian reference center for the research, diagnosis, training and education on IEM. During the last 20 years, important advances have been achieved in the clinical knowledge of these disorders, as well as in the local availability of several diagnosis tests. Organic acidurias have been the most frequently detected diseases, followed by aminoacidopathies and peroxisomal disorders. Research efforts have been focused in the production of recombinant proteins in microorganisms towards the development of new enzyme replacement therapies, the design of gene therapy vectors and the use of bioinformatics tools for the understanding of IEM. In addition, this center has participated in the education and training of a large number professionals at different levels, which has contributed to increase the knowledge and divulgation of these disorders along the country. Noteworthy, in close collaboration with patient advocacy groups, we have participated in the discussion and construction of initiatives for the inclusion of diagnosis tests and treatments in the health system.
- Published
- 2018
- Full Text
- View/download PDF
6. Bimetallic CoMoS Composite Anchored to Biocarbon Fibers as a High-Capacity Anode for Li-Ion Batteries
- Author
-
Noemi Dominguez, Brenda Torres, Luis A. Barrera, Julio E. Rincon, Yirong Lin, Russell R. Chianelli, Md. Ariful Ahsan, and Juan C. Noveron
- Subjects
Chemistry ,QD1-999 - Published
- 2018
- Full Text
- View/download PDF
7. Pairwise library screen systematically interrogates Staphylococcus aureus Cas9 specificity in human cells
- Author
-
Josh Tycko, Luis A. Barrera, Nicholas C. Huston, Ari E. Friedland, Xuebing Wu, Jonathan S. Gootenberg, Omar O. Abudayyeh, Vic E. Myer, Christopher J. Wilson, and Patrick D. Hsu
- Subjects
Science - Abstract
A rigorous understanding of off-target effects is necessary for SaCas9 to be used in therapeutic genome editing. Here the authors measure SaCas9 mismatch tolerance across a pairwise library screen of 88,000 guides and targets in human cells and develop a model which ranks off-target sites.
- Published
- 2018
- Full Text
- View/download PDF
8. UDiTaS™, a genome editing detection method for indels and genome rearrangements
- Author
-
Georgia Giannoukos, Dawn M. Ciulla, Eugenio Marco, Hayat S. Abdulkerim, Luis A. Barrera, Anne Bothmer, Vidya Dhanapal, Sebastian W. Gloskowski, Hariharan Jayaram, Morgan L. Maeder, Maxwell N. Skor, Tongyao Wang, Vic E. Myer, and Christopher J. Wilson
- Subjects
Gene editing ,CRISPR/CAS9 ,Next generation sequencing ,NGS ,UDiTaS ,Translocation detection ,Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Understanding the diversity of repair outcomes after introducing a genomic cut is essential for realizing the therapeutic potential of genomic editing technologies. Targeted PCR amplification combined with Next Generation Sequencing (NGS) or enzymatic digestion, while broadly used in the genome editing field, has critical limitations for detecting and quantifying structural variants such as large deletions (greater than approximately 100 base pairs), inversions, and translocations. Results To overcome these limitations, we have developed a Uni-Directional Targeted Sequencing methodology, UDiTaS, that is quantitative, removes biases associated with variable-length PCR amplification, and can measure structural changes in addition to small insertion and deletion events (indels), all in a single reaction. We have applied UDiTaS to a variety of samples, including those treated with a clinically relevant pair of S. aureus Cas9 single guide RNAs (sgRNAs) targeting CEP290, and a pair of S. pyogenes Cas9 sgRNAs at T-cell relevant loci. In both cases, we have simultaneously measured small and large edits, including inversions and translocations, exemplifying UDiTaS as a valuable tool for the analysis of genome editing outcomes. Conclusions UDiTaS is a robust and streamlined sequencing method useful for measuring small indels as well as structural rearrangements, like translocations, in a single reaction. UDiTaS is especially useful for pre-clinical and clinical application of gene editing to measure on- and off-target editing, large and small.
- Published
- 2018
- Full Text
- View/download PDF
9. Characterization of the interplay between DNA repair and CRISPR/Cas9-induced DNA lesions at an endogenous locus
- Author
-
Anne Bothmer, Tanushree Phadke, Luis A. Barrera, Carrie M Margulies, Christina S. Lee, Frank Buquicchio, Sean Moss, Hayat S. Abdulkerim, William Selleck, Hariharan Jayaram, Vic E. Myer, and Cecilia Cotta-Ramusino
- Subjects
Science - Abstract
CRISPR-Cas9 has rapidly become a common molecular biology tool for modifying genomes and has been modified to generate single-strand nicks as well as double-strand breaks. Here the authors explore the DNA repair pathways activated by the different variants of Cas9.
- Published
- 2017
- Full Text
- View/download PDF
10. Growth Plate Pathology in the Mucopolysaccharidosis Type VI Rat Model—An Experimental and Computational Approach
- Author
-
Johana M. Guevara-Morales, Michael Frohbergh, Hector Castro-Abril, Juan J. Vaca-González, Luis A. Barrera, Diego A. Garzón-Alvarado, Edward Schuchman, and Calogera Simonaro
- Subjects
mucopolysaccharidosis type VI ,growth plate histology ,columnar arrangement ,skeletal dysplasia ,growth plate computational model ,Medicine (General) ,R5-920 - Abstract
Background: Mucopolysaccharidoses (MPS) are a group of inherited metabolic diseases caused by impaired function or absence of lysosomal enzymes involved in degradation of glycosaminoglycans. Clinically, MPS are skeletal dysplasias, characterized by cartilage abnormalities and disturbances in the process of endochondral ossification. Histologic abnormalities of growth cartilage have been reported at advanced stages of the disease, but information regarding growth plate pathology progression either in humans or in animal models, as well as its pathophysiology, is limited. Methods: Histological analyses of distal femur growth plates of wild type (WT) and mucopolysaccharidosis type VI (MPS VI) rats at different stages of development were performed, including quantitative data. Experimental findings were then analyzed in a theoretical scenario. Results: Histological evaluation showed a progressive loss of histological architecture within the growth plate. Furthermore, in silico simulation suggest the abnormal cell distribution in the tissue may lead to alterations in biochemical gradients, which may be one of the factors contributing to the growth plate abnormalities observed, highlighting aspects that must be the focus of future experimental works. Conclusion: The results presented shed some light on the progression of growth plate alterations observed in MPS VI and evidence the potentiality of combined theoretical and experimental approaches to better understand pathological scenarios, which is a necessary step to improve the search for novel therapeutic approaches.
- Published
- 2020
- Full Text
- View/download PDF
11. The NF-κB Genomic Landscape in Lymphoblastoid B Cells
- Author
-
Bo Zhao, Luis A. Barrera, Ina Ersing, Bradford Willox, Stefanie C.S. Schmidt, Hannah Greenfeld, Hufeng Zhou, Sarah B. Mollo, Tommy T. Shi, Kaoru Takasaki, Sizun Jiang, Ellen Cahir-McFarland, Manolis Kellis, Martha L. Bulyk, Elliott Kieff, and Benjamin E. Gewurz
- Subjects
Biology (General) ,QH301-705.5 - Abstract
The nuclear factor κB (NF-κΒ) subunits RelA, RelB, cRel, p50, and p52 are each critical for B cell development and function. To systematically characterize their responses to canonical and noncanonical NF-κB pathway activity, we performed chromatin immunoprecipitation followed by high-throughput DNA sequencing (ChIP-seq) analysis in lymphoblastoid B cell lines (LCLs). We found a complex NF-κB-binding landscape, which did not readily reflect the two NF-κB pathway paradigms. Instead, 10 subunit-binding patterns were observed at promoters and 11 at enhancers. Nearly one-third of NF-κB-binding sites lacked κB motifs and were instead enriched for alternative motifs. The oncogenic forkhead box protein FOXM1 co-occupied nearly half of NF-κB-binding sites and was identified in protein complexes with NF-κB on DNA. FOXM1 knockdown decreased NF-κB target gene expression and ultimately induced apoptosis, highlighting FOXM1 as a synthetic lethal target in B cell malignancy. These studies provide a resource for understanding mechanisms that underlie NF-κB nuclear activity and highlight opportunities for selective NF-κB blockade.
- Published
- 2014
- Full Text
- View/download PDF
12. Ecogeography and Climate Change in Forage Grasses from Arid and Semi-Arid Regions of Mexico
- Author
-
Gabriela Ramírez-Ojeda, Edith Ramírez-Segura, Luis Ángel Barrera-Guzmán, and Abieser Vázquez-González
- Subjects
ecogeography ,forage grasses ,climate change ,arid and semi-arid regions ,Plant culture ,SB1-1110 - Abstract
Arid and semi-arid areas are highly productive ecosystems that have a great diversity of species, among which grasses stand out. These species have ecological, environmental, and economic importance. The objective was to identify the ecological descriptors of 15 native species of grasses and diversity patterns through multivariate analysis techniques (principal component analysis, PCA, and cluster analysis, CA), in addition to identifying potential distribution areas (current and future) and regions of high and low diversity from 3841 accessions and 21 climatic variables. Among the main results, the extreme values, coefficient of variation, and median for each species could be determined. PCA determined that, with 10 variables, it was possible to explain 54.36% of the variation between the analyzed species. CA resulted in the formation of four statistically significant groups, with specific climatic characteristics. Regarding potential distribution areas and climate change, a reduction in distribution is expected (2050) on most of the current surface, with a possible opportunity zone in the north of the country. Finally, two hotspots (high diversity areas) and four coldspots (low diversity areas) were identified among the analyzed species. These results can be used to create strategies for sustainable use and the conservation of these resources.
- Published
- 2024
- Full Text
- View/download PDF
13. Publisher Correction: Pairwise library screen systematically interrogates Staphylococcus aureus Cas9 specificity in human cells
- Author
-
Josh Tycko, Luis A. Barrera, Nicholas C. Huston, Ari E. Friedland, Xuebing Wu, Jonathan S. Gootenberg, Omar O. Abudayyeh, Vic E. Myer, Christopher J. Wilson, and Patrick D. Hsu
- Subjects
Science - Abstract
The original HTML version of this Article incorrectly listed an affiliation of Josh Tycko as ‘Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA’, instead of the correct ‘Present address: Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA’. It also incorrectly listed an affiliation of this author as ‘Present address: Arrakis Therapeutics, 35 Gatehouse Dr., Waltham, MA, 02451, USA’.The original HTML version incorrectly listed an affiliation of Luis A. Barrera as ‘Present address: Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, 06511, USA’, instead of the correct ‘Present address: Arrakis Therapeutics, 35 Gatehouse Dr., Waltham, MA 02451, USA’.Finally, the original HTML version incorrectly omitted an affiliation of Nicholas C. Huston: ‘Present address: Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511, USA’.This has been corrected in the HTML version of the Article. The PDF version was correct from the time of publication.
- Published
- 2018
- Full Text
- View/download PDF
14. Predicción computacional de la estructura terciaria de la iduronato 2-sulfato sulfatasa humana
- Author
-
Homero Sáenz, Leonardo Lareo, Raúl A. Poutou, Ángela C. Sosa, and Luis A. Barrera
- Subjects
mucopolysaccharidosis II ,iduronate sulfatase ,tertiary protein structure ,computing methods ,Medicine ,Arctic medicine. Tropical medicine ,RC955-962 - Abstract
Introducción. El síndrome de Hunter o mucopolisacaridosis tipo II (MC KUSIK 309900) es causado por la deficiencia de la iduronato 2-sulfato sulfatasa humana (E.C. 3.1.6.13). La enzima no ha sido cristalizada y por tanto sus estructuras no se conocen por deducción experimental. Objetivo. Proponer un modelo computacional para la estructura tridimensional de la iduronato 2-sulfato sulfatasa humana. Materiales y métodos. Se realizó un análisis computacional de esta enzima empleando programas de libre acceso en internet como Comput pI/MW, JaMBW Chapter 3.1.7, SWISSMODEL, 3D-PSSM, ProSup. Los procesos de minimización de energía se realizaron con el programa Discover 3 del paquete Insight II (2004). Resultados. Se propone un modelo tridimensional de la iduronato 2-sulfato sulfatasa humana que presenta 33,3% en hélice, 7,2% en hoja plegada y 59,5% en enrollamiento al azar (coil). Se hallaron valores de RMS (del inglés Root Mean Square) de 0,78 y 0,86Å al compararla con otras enzimas de la misma familia. El modelo revela cinco sitios potenciales de N-glicosilación y una entrada al bolsillo que contiene los aminoácidos que componen el sitio activo. Usando este modelo se encontró una buena correlación entre el tipo de mutaciones y la gravedad de la enfermedad en 20 pacientes analizados. Conclusión. Los valores de RMS y la correlación genotipo-fenotipo en los pacientes analizados sugieren el modelo puede usarse para predecir ciertos aspectos del comportamiento biológico de la enzima.
- Published
- 2007
- Full Text
- View/download PDF
15. Producción de anticuerpos policlonales IgG contra la proteina iduronato-2-sulfato sulfatasa y desarrollo de un sistema de detección para IDS humana recombinante.
- Author
-
Olga Peña, Angela Sosa, Olga Echeverri, Homero Sáenz, and Luis A. Barrera
- Subjects
mucopolysaccharidosis ,IDS ,Hunter syndrome ,ERT ,polyclonal antibodies ,Medicine ,Arctic medicine. Tropical medicine ,RC955-962 - Abstract
Introducción. La enfermedad de Hunter es un trastorno lisosómico caracterizado por la deficiencia de la enzima iduronato-2-sulfato sulfatasa (IDS) (EC 3.1.6.13). Esta enfermedad, al igual que muchos trastornos metabólicos, son patologías intratables mediante la terapéutica convencional; sin embargo, existe la posibilidad de ser tratada alternativamente mediante terapia génica o terapia de reemplazo enzimático. Objetivo. El Instituto de Errores Innatos del Metabolismo (IEIM) ha desarrollado un sistema de expresión de sulfatasas para producir IDS humana recombinante (IDShr) en Escherichia coli y Pichia pastoris, con resultados favorables. El objetivo principal de este trabajo fue desarrollar un sistema de detección de IDS humana recombinante. Materiales y métodos. Para el efecto, se inmunizaron con IDS comercial de TKT (Cambridge, MA) dos conejos de raza Nueva Zelanda blanca y los anticuerpos purificados a partir del suero se utilizaron en el desarrollo de una técnica semicuantitativa por dot-blot. Diferentes muestras de extractos crudos de fermentaciones con P. pastoris y E. coli se procesaron con el fin de poder determinar la presencia de la enzima. Resultados. Se demostró que los anticuerpos eran específicos en el reconocimiento de la IDS sin presentar reactividad cruzada con proteínas contaminantes de los extractos crudos. Conclusión. Por consiguiente, los anticuerpos se podrán usar en el desarrollo de una técnica ELISA tipo sandwich como método de detección y cuantificación de la enzima y en procesos de purificación de la misma mediante cromatografía de afinidad.
- Published
- 2005
- Full Text
- View/download PDF
16. Streptomyces as a Novel Biotool for Azo Pigments Remediation in Contaminated Scenarios
- Author
-
Fernando Bautista-Pinzón, Juan Fonseca-Ordoñez, Mayerlen Falla-Obando, Jairo Gonzales-Tuta, and Luis Diaz-Barrera
- Subjects
bioremediation ,streptomyces ,azo pigments ,isolation sources ,encapsulation ,biodegradation ,Environmental sciences ,GE1-350 ,Microbiology ,QR1-502 - Abstract
Background: Azo pigments are widely used in the textile and leather industry, and they generate diverse contaminants (mainly in wastewater effluents) that affect biological systems, the rhizosphere community, and the natural activities of certain species. Methods: This review was performed according to the Systematic Reviews and Meta Analyses (PRISMA) methodology. Results: In the last decade, the use of Streptomyces species as biological azo-degraders has increased, and these bacteria are mainly isolated from mangroves, dye-contaminated soil, and marine sediments. Azo pigments such as acid orange, indigo carmine, Congo red, and Evans blue are the most studied compounds for degradation, and Streptomyces produces extracellular enzymes such as peroxidase, laccase, and azo reductase. These enzymes cleave the molecule through asymmetric cleavage, followed by oxidative cleavage, desulfonation, deamination, and demethylation. Typically, some lignin-derived and phenolic compounds are used as mediators to improve enzyme activity. The degradation process generates diverse compounds, the majority of which are toxic to human cells and, in some cases, can improve the germination process in some horticulture plants. Conclusions: Future research should include analytical methods to detect all of the molecules that are generated in degradation processes to determine the involved reactions. Moreover, future studies should delve into consortium studies to improve degradation efficiency and observe the relationship between microorganisms to generate scale-up biotechnological applications in the wastewater treatment industry.
- Published
- 2024
- Full Text
- View/download PDF
17. Cytosine base editing inhibits hepatitis B virus replication and reduces HBsAg expression in vitro and in vivo
- Author
-
Elena M. Smekalova, Maria G. Martinez, Emmanuel Combe, Anuj Kumar, Selam Dejene, Dominique Leboeuf, Chao-Ying Chen, J. Robert Dorkin, Lan Shuan Shuang, Sarah Kieft, Lauren Young, Luis Alberto Barrera, Michael S. Packer, Giuseppe Ciaramella, Barbara Testoni, Francine Gregoire, and Fabien Zoulim
- Subjects
MT: RNA/DNA Editing ,HBV ,cccDNA ,base editing ,HBsAg ,therapeutics ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Chronic hepatitis B virus (HBV) infection remains a global health problem due to the lack of treatments that prevent viral rebound from HBV covalently closed circular (ccc)DNA. In addition, HBV DNA integrates in the human genome, serving as a source of hepatitis B surface antigen (HBsAg) expression, which impairs anti-HBV immune responses. Cytosine base editors (CBEs) enable precise conversion of a cytosine into a thymine within DNA. In this study, CBEs were used to introduce stop codons in HBV genes, HBs and Precore. Transfection with mRNA encoding a CBE and a combination of two guide RNAs led to robust cccDNA editing and sustained reduction of the viral markers in HBV-infected HepG2-NTCP cells and primary human hepatocytes. Furthermore, base editing efficiently reduced HBsAg expression from HBV sequences integrated within the genome of the PLC/PRF/5 and HepG2.2.15 cell lines. Finally, in the HBV minicircle mouse model, using lipid nanoparticulate delivery, we demonstrated antiviral efficacy of the base editing approach with a >3log10 reduction in serum HBV DNA and >2log10 reduction in HBsAg, and 4/5 mice showing HBsAg loss. Combined, these data indicate that base editing can introduce mutations in both cccDNA and integrated HBV DNA, abrogating HBV replication and silencing viral protein expression.
- Published
- 2024
- Full Text
- View/download PDF
18. Descripción de polimorfismos genéticos en CYP3A5 y MDR-1 y su impacto en el rechazo agudo clínico de pacientes trasplantados de hígado del Hospital San Vicente Fundación Rionegro
- Author
-
Lina María Botero-Mora, Erika Fernanda Lindarte-Rincón, Luis Manuel Barrera-Lozano, Jaime Alberto Ramírez-Arbeláez, Jefferson Antonio Buendía, and Luis Guillermo Toro-Rendón
- Subjects
trasplante de órganos ,trasplante de hígado ,rechazo de injerto ,polimorfismo de nucleótido simple ,tacrolimus ,farmacogenética ,Surgery ,RD1-811 - Abstract
Introducción. El tacrolimus es un medicamento inmunosupresor ampliamente usado en trasplante hepático, que presenta una gran variabilidad interindividual la cual se considera asociada a la frecuencia de polimorfismos de CYP3A5 y MDR-1. El objetivo de este estudio fue evaluar la frecuencia de los polimorfismos rs776746, rs2032582 y rs1045642 y su asociación con rechazo clínico y toxicidad farmacológica. Métodos. Se incluyeron pacientes inmunosuprimidos con tacrolimus a quienes se les realizó trasplante hepático en el Hospital San Vicente Fundación Rionegro entre 2020 y 2022, con supervivencia mayor a un mes. Se evaluaron las variables clínicas, rechazo agudo y toxicidad farmacológica. Se secuenciaron los genes de estudio mediante PCR, comparando la expresión o no en cada uno de los pacientes. Resultados. Se identificaron 17 pacientes. El 43 % de los pacientes se clasificaron como CYP3A5*1/*1 y CYP3A5*1/*3, entre los cuales se encontró asociación con aumento en la tasa de rechazo agudo clínico, al comparar con los pacientes no expresivos (100 % vs. 44 %, p=0,05); no hubo diferencias en cuanto a la toxicidad farmacológica u otros desenlaces. Se encontró el polimorfismo rs2032582 en un 50 % y el rs1045642 en un 23,5 % de los pacientes, sin embargo, no se identificó asociación con rechazo u otros eventos clínicos. Conclusiones. Se encontró una asociación entre el genotipo CYP3A5*1/*1 y CYP3A5*1/*3 y la tasa de rechazo clínico. Sin embargo, se requiere una muestra más amplia para validar estos datos y plantear modelos de medicina personalizada.
- Published
- 2024
- Full Text
- View/download PDF
19. Influencia de la fuente de carbono sobre la expresión de proteínas AOX1-reguladas en Pichia pastoris
- Author
-
Raúl A. Poutou, Balkys E. Quevedo, Henrry A. Córdoba, Homero Sáenz Sáenz, and Luis A. Barrera
- Subjects
Pichia pastoris ,AOX ,Represión catabólica ,Autofagia ,Fuente de carbono ,Peroxisoma ,Medicine (General) ,R5-920 ,Biology (General) ,QH301-705.5 - Abstract
La levadura Pichia pastoris constituye un excelente modelo para la expresión de proteínas heterólogas, lo que se refleja en el gran número de proteínas que han sido obtenidas en este modelo biológico, bajo el control del promotor AOX1. Esto implica que el número de peroxisomas y la enzima alcohol oxidasa es regulada como respuesta a la inducción por metabolitos como el metanol, el glicerol, el etanol, el acetato y vías metabólica como la b-oxidación. En esta revisión, se discuten aspectos relacionados con el metabolismo de estos compuestos y su posible influencia en la producción de proteínas recombinantes, específicamente la Iduronato 2- sulfato sulfatasa humana (IDSh).
- Published
- 2005
- Full Text
- View/download PDF
20. SAS Classification in the creation of new companies in Ecuador.
- Author
-
Daniel Mosquera, Fabricio Echeverria, Marcelo León, Luis Torres-Barrera, and Eladio Freire
- Published
- 2023
21. Recoil Measurements in Drosophila Embryos: from Mounting to Image Analysis
- Author
-
Luis Sánchez-Cisneros, Sourabh Bhide, and Luis Ríos-Barrera
- Subjects
Biology (General) ,QH301-705.5 - Abstract
Tension and force propagation play a central role in tissue morphogenesis, as they enable sub- and supra-cellular shape changes required for the generation of new structures. Force is often generated by the cytoskeleton, which forms complex meshworks that reach cell–cell or cell–extracellular matrix junctions to induce cellular rearrangements. These mechanical properties can be measured through laser microdissection, which concentrates energy in the tissue of interest, disrupting its cytoskeleton. If the tissue is undergoing tension, this cut will induce a recoil in the surrounding regions of the cut. This protocol describes how one can perform laser microdissection experiments and subsequently measure the recoil speed of the sample of interest. While we explain how to carry out these experiments in Drosophila embryos, the recoil calibration and downstream analyses can be applied to other types of preparations. Key features• Allows measuring tension in live Drosophila embryos with a relatively simple approach. • Describes a quick way to mount a high number of embryos.• Includes a segmentation-free recoil quantification that reduces bias and speeds up analysis.Graphical overview
- Published
- 2023
- Full Text
- View/download PDF
22. Tuberculosis Severity Predictive Model Using Mtb Variants and Serum Biomarkers in a Colombian Cohort of APTB Patients
- Author
-
Juan C. Ocampo, Juan F. Alzate, Luis F. Barrera, and Andres Baena
- Subjects
Mycobacterium tuberculosis ,severity model ,CHIT1 ,tuberculosis ,Biology (General) ,QH301-705.5 - Abstract
Currently, tuberculosis (TB) is a bacterial infection caused by Mycobacterium tuberculosis (Mtb) that primarily affects the lungs. The severity of active pulmonary TB (APTB) is an important determinant of transmission, morbidity, mortality, disease experience, and treatment outcomes. Several publications have shown a high prevalence of disabling complications in individuals who have had severe APTB. Furthermore, certain strains of Mtb were associated with more severe disease outcomes. The use of biomarkers to predict severe APTB patients who are candidates for host-directed therapies, due to the high risk of developing post-tuberculous lung disease (PTLD), has not yet been implemented in the management of TB patients. We followed 108 individuals with APTB for 6 months using clinical tools, flow cytometry, and whole-genome sequencing (WGS). The median age of the study population was 26.5 years, and the frequency of women was 53.7%. In this study, we aimed to identify biomarkers that could help us to recognize individuals with APTB and improve our understanding of the immunopathology in these individuals. In this study, we conducted a follow-up on the treatment progress of 121 cases of APTB. The follow-up process commenced at the time of diagnosis (T0), continued with a control visit at 2 months (T2), and culminated in an exit appointment at 6 months following the completion of medical treatment (T6). People classified with severe APTB showed significantly higher levels of IL-6 (14.7 pg/mL; p < 0.05) compared to those with mild APTB (7.7 pg/mL) at T0. The AUCs for the ROC curves and the Matthews correlation coefficient values (MCC) demonstrate correlations ranging from moderate to very strong. We conducted WGS on 88 clinical isolates of Mtb, and our analysis revealed a total of 325 genes with insertions and deletions (Indels) within their coding regions when compared to the Mtb H37Rv reference genome. The pattern of association was found between serum levels of CHIT1 and the presence of Indels in Mtb isolates from patients with severe APTB. A key finding in our study was the high levels of CHIT1 in severe APTB patients. We identified a biomarker profile (IL-6, IFN-γ, IL-33, and CHIT1) that allows us to identify individuals with severe APTB, as well as the identification of a panel of polymorphisms (125) in clinical isolates of Mtb from individuals with severe APTB. Integrating these findings into a predictive model of severity would show promise for the management of APTB patients in the future, to guide host-directed therapy and reduce the prevalence of PTLD.
- Published
- 2023
- Full Text
- View/download PDF
23. Análisis de uma cartera de inversión, basada em los princípios del metodo de inversión em valor (value investing), e inversión focalizada (focus investing): un estudio de caso
- Author
-
Baqueiro, Gustavo Alberto Barredo, primary, Ramírez, Luis Martín Barrera, additional, and Novelo, Valentín Alonso, additional
- Published
- 2022
- Full Text
- View/download PDF
24. La criptología de la enfermedad: Cómo aprendimos a descifrar y corregir enfermedades heredadas
- Author
-
Luis Alejandro Barrera Avellaneda and Luis Alejandro Barrera Avellaneda
- Published
- 2021
25. A systematic evaluation of Mycobacterium tuberculosis Genome-Scale Metabolic Networks.
- Author
-
Víctor A. López-Agudelo, Tom A. Mendum, Emma Laing, HuiHai Wu, Andres Baena, Luis F. Barrera, Dany J. V. Beste, and Rigoberto Ríos-Estepa
- Published
- 2020
- Full Text
- View/download PDF
26. Fuzzy Logic Control With an Improved Algorithm for Integrated LED Drivers.
- Author
-
René Osorio, J. Marcos Alonso, Nimrod Vázquez, Sergio Pinto, Felipe De Jesus Sorcia-Vazquez, Mario Martínez-García, and Luis Manuel Barrera
- Published
- 2018
- Full Text
- View/download PDF
27. Colitis actínica de localización cecal. Serie de casos
- Author
-
Maria Margarita Rojas-Rojas, Catalina Buritica, Luis Eduardo Barrera Herrera, Marcela Mejia-Arango, David A Suarez-Zamora, and Johanna Alvarez
- Subjects
Colitis ,radioterapia ,hemorragia gastrointestinal ,Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Por definición, la colitis actínica incluye cambios inflamatorios de la mucosa colorrectal secundarios a radioterapia en cercanía a la región tratada. La localización más frecuente es el recto y la indicación más común de radioterapia corresponde a neoplasias de la región pélvica incluidos el recto, la próstata y el cérvix. Se estima que hasta la mitad de los pacientes que reciben radiación pélvica llega a desarrollar síntomas gastrointestinales asociados. Se presentan dos pacientes con metástasis óseas sacroilíacas y pélvicas secundarias a adenocarcinoma de próstata que recibieron radiación en la región lumbosacra y pélvica, ambos pacientes presentaron episodios de deposiciones con sangre que iniciaron tempranamente posterior a la radioterapia. La colonoscopia mostró eritema y ulceración. En el estudio histopatológico se observó un patrón de colitis isquémica, con núcleo y citomegalia, estroma fibroso con cambios reactivos y abundante infiltrado inflamatorio neutrofílico. Estos hallazgos son característicos de la colitis actínica aguda; sin embargo, la localización cecal no ha sido frecuentemente reportada. Aunque al ser el ciego y el íleon terminal móviles de localización pélvica, se convierten en un factor de riesgo para que estos segmentos anatómicos sean susceptibles al impacto directo de la radioterapia. Esta condición, en la fase aguda, es autolimitada y se suele resolver con medidas de soporte. Es indispensable que el personal involucrado en el manejo de estos pacientes conozca esta entidad y los posibles diagnósticos diferenciales.
- Published
- 2020
- Full Text
- View/download PDF
28. Comparación de perfiles multiméricos del factor de von Willebrand obtenidos mediante un ensayo electroforético comercial y electroforesis local con geles de agarosa 1%
- Author
-
Marina Sol Lopez, Juvenal Paiva, Adriana Woods, María Soledad Saez, Luis Horacio Barrera, Verónica Privitera, Fernando Chuliber, Maximiliano Villagra Iturre, Diana Penchasky, Patricia Sorroche, Marta Martinuzzo, and Analia Sánchez Luceros
- Abstract
La enfermedad de von Willebrand (VWD) es el desorden hemorrágico congénito más frecuente y se produce por la deficiencia y/o alteración del factor von Willebrand (VWF). El VWF es una glicoproteí-na multimérica que se sintetiza en las células endo-teliales y megacariocitos y se almacena en las orga-nelas de depósito: los cuerpos de Weibel Palade en las células endoteliales y los α-gránulos plaquetarios. Además, este factor circula en el plasma unido al factor VIII, protegiéndolo de la proteólisis mediada por inhibidores fisiológicos. La unidad funcional está conformada por un monómero, que luego dimeriza, a través de uniones S-S en el dominio VWF-CK, en el extremo C-terminal. Luego de sucesivos pasos de O- y N-glicosilación, se produce la unión de los dí-meros en multímeros, mediante uniones S-S entre el dominio VWF-D3. Los multímeros de VWF inclu-yen formas de peso molecular bajo (LMWM), inter-medio (IMWM), alto (HMWM) y formas de peso molecular ultragrandes (UHMWM). El VWF almacenado en las organelas de depósito y posteriormente liberado por la vía secretoria es rico en UHMWM, mientras que el VWF plasmático secretado constitutivamente presenta multímeros pequeños, intermedios y grandes. Los UHMWM son altamente trombogénicos y son degradados, una vez liberados, por la metaloproteasa plasmática ADAMTS13, en-zima que cliva específicamente al VWF entre los residuos Tyr1605-Met1606 localizados en el dominio VWF-A2.
- Published
- 2022
29. Incisional Hernia in Subxiphoid Trocar Port in Cholecystectomized Patient
- Author
-
Maya, Gabriela Martínez, Romero, Arturo Rojas, Rosas, José Manuel Pastrana, Carbajal, José Sotelo, Galan, Luis Manuel Uribe, and Guerra, Luis Rodrigo Barrera
- Subjects
Laparoscopic cholecystectomy ,incisional hernia ,subxiphoid port, mesh - Abstract
Laparoscopic surgery is a minimal access procedure that maintains the integrity of the abdominal wall, however, it is not exempt from complications, such as vascular and intestinal injuries and the rarest complication: incisional hernia of the trocar port with an incidence of 0.65. -There are risk factors related to the appearance of trocar port hernias, they are divided into those associated with the surgical technique and the characteristics of the patient. We present a clinical case of a patient with an incisional hernia in the laparoscopic trocar port with a subxiphoid location due to cholecystectomy, which was repaired with polypropylene mesh.
- Published
- 2023
- Full Text
- View/download PDF
30. Portal Vein Thrombosis in Liver Transplantation: A Retrospective Cohort Study
- Author
-
Ardila, Luis Manuel Barrera-Lozano, Jaime Alberto Ramírez-Arbeláez, Cristian Leonardo Muñoz, Jorge Andrés Becerra, Luis Guillermo Toro, and Carlos M.
- Subjects
liver transplantation ,liver cirrhosis ,portal vein thrombosis ,survival - Abstract
Portal vein thrombosis was considered a contraindication for liver transplantation. This study analyzes the perioperative complications and survival of liver transplant patients with portal vein thrombosis (PVT). A retrospective observational cohort study of liver transplant patients was conducted. The outcomes were early mortality (30 days) and patient survival. A total of 201 liver transplant patients were identified and 34 (17%) patients with PVT were found. The most frequent extension of thrombosis was Yerdel 1 (58.8%), and a portosystemic shunt was identified in 23 (68%) patients. Eleven patients (33%) presented any early vascular complication, PVT being the most frequent (12%). The multivariate regression analysis showed a statistically significant association between PVT and early complications (OR = 3.3, 95% confidence interval 1.4–7.7; p = 0.006). Moreover, early mortality was observed in eight patients (24%), of which two (5.9%) presented Yerdel 2. For Yerdel 1, patient survival according to the extent of thrombosis was 75% at 1 year and 3 years, while for Yerdel 2, it was 65% at 1 year, and 50% at 3 years (p = 0.04). Portal vein thrombosis significantly influenced early vascular complications. Furthermore, portal vein thrombosis Yerdel 2 or higher impacts the survival of liver grafts in the short and long term.
- Published
- 2023
- Full Text
- View/download PDF
31. High-efficiency base editing for Stargardt disease in mice, non-human primates, and human retina tissue
- Author
-
Alissa Muller, Jack Sullivan, Wibke Schwarzer, Mantian Wang, Cindy Park-Windhol, Beryll Klingler, Jane Matsell, Simon Hostettler, Patricia Galliker, Mert Duman, Yanyan Hou, Pierre Balmer, Tamás Virág, Luis Alberto Barrera, Quan Xu, Dániel Péter Magda, Ferenc Kilin, Arogya Khadka, Mathieu Quinodoz, Pascal W. Hasler, Pierre-Henri Moreau, Lyne Fellmann, Thierry Azoulay, Marco Cattaneo, Simone Picelli, Alice Grison, Cameron S. Cowan, Lucas Janeschitz-Kriegl, Ákos Kusnyerik, Magdalena Renner, Zoltán Zsolt Nagy, Arnold Szabó, Carlo Rivolta, Hendrik P.N. Scholl, David Bryson, Giuseppe Ciaramella, Botond Roska, and Bence György
- Abstract
Stargardt disease is a currently untreatable, inherited neurodegenerative disease that leads to macular degeneration and blindness due to loss-of-function mutations in theABCA4gene. We have designed a dual adeno-associated viral vector split-intein adenine base-editing strategy to correct the most common mutation inABCA4(c.5882G>A, p.G1961E). We optimizedABCA4base editing in human models, including retinal organoids, iPSC-derived retinal pigment epithelial (RPE) cells, as well as adult human retinal- and RPE/choroid explants in vitro. The resulting gene therapy vectors achieved high levels of gene correction in mutation-carrying mice and in non-human primates, with an average editing of 37% of photoreceptors and 73% of RPE cells in vivo. The high editing rates in primates make way for precise and efficient gene editing in other neurodegenerative ocular diseases.
- Published
- 2023
32. Mesohepatectomy, an alternative for the management of hepatocellular carcinoma in non-cirrhotic patient: case series
- Author
-
Luis Manuel Barrera-Lozano, Jaime Alberto Ramírez, Jorge Andrés Becerra, Cristian Leonardo Muñoz, Diana Catalina Pineda Pineda, and Jorge Iván Gutiérrez-Montoya
- Subjects
RD1-811 ,liver failure ,Sepsis postoperatoria ,hepatectomía ,falla hepática ,hepatic cirrhosis ,extendida ,extended ,cirrosis hepática ,hepatectomy ,hepatocarcinoma ,central ,postoperative ,Surgery ,postoperatoria - Abstract
Resumen Introducción. La resección quirúrgica es el tratamiento de elección de las neoplasias primarias y secundarias del hígado. Los pacientes con hepatocarcinoma de los segmentos centrales representan un reto, siendo la hepatectomía extendida la técnica más usada, sin embargo, el riesgo postquirúrgico de falla hepática es alto, dado que la resección puede comprometer entre el 65 % y el 80 % del volumen hepático. La mesohepatectomía es una alternativa que permite dejar un volumen hepático residual suficiente. El objetivo de este trabajo es presentar nuestra experiencia en el tratamiento de pacientes con hepatocarcinomas en segmentos centrales a quienes se les realizó mesohepatectomía. Serie de casos. Se presentan tres pacientes no cirróticos, con hepatocarcinoma en los segmentos 4, 5 y 8, que fueron atendidos en el Hospital San Vicente Fundación, en las sedes de Medellín y de Rionegro, entre 2018 y 2020. Resultados. La mesohepatectomía se realizó mediante ligadura selectiva de los pedículos del segmento 4 y del sector anterior derecho. Se utilizó aspirador ultrasónico y endograpadora para la transección hepática. La duración de la maniobra de Pringle varió entre 16 y 43 minutos. El sangrado promedio fue de 1000 ml. Solo un paciente presentó fuga biliar tipo B. No hubo mortalidad a 30 días. Conclusiones. La mesohepatectomía es una alternativa segura para pacientes con tumores en los segmentos centrales, que permite disminuir el riesgo de falla hepática luego de la resección. Abstract Introduction. Surgical resection is the treatment of choice for primary and secondary neoplasms of the liver. Patients with central segment hepatocarcinoma represent a challenge, with extended hepatectomy being the most widely used technique. However, the postsurgical risk of liver failure is high since resection can compromise between 65% and 80% of liver volume. Mesohepatectomy is an alternative that allows a sufficient residual liver volume to be left. The objective of this work is to present treatment of patients with central segment hepatocarcinoma. Clinical cases. Three non-cirrhotic patients are presented, with hepatocarcinoma in segments 4, 5 and 8, who were treated at the San Vicente Fundación Hospital in Medellín and Rionegro, between 2018 and 2020. Results. Mesohepatectomy was performed by selective ligation of the pedicles of segment 4 and the right anterior sector. An ultrasonic aspirator and endostapler were used for liver transection. The duration of the Pringle maneuver ranged from 16 to 43 minutes. The average bleeding was 1000 cc. Only one patient had type B bile leakage. There was no 30-day mortality. Conclusions. Mesohepatectomy is a safe alternative for patients with tumors in the central segments, which reduces the risk of liver failure after resection.
- Published
- 2021
33. La criptología de enfermedad
- Author
-
Luis Alejandro Barrera
- Abstract
Intervención del Académico Jaime Bernal Villegas, MD, PhD. en la sesión académica del 18 de noviembre de 2021 He leído con extraordinario interés el libro La Criptología de la enfermedad. Cómo aprendimos a descifrar y corregir enfermedades heredadas, cuyo autor es el doctor y académico Luis Alejandro Barrera, y que es la razón para estar congregados aquí, en la Academia Nacional de Medicina esta noche. Los errores congénitos del metabolismo son un grupo enorme, tal vez mas de 500 entidades, producto de disrupciones en alguna de las vías metabólicas de los seres humanos y de otra cantidad de animales que comparten con nosotros patrones metabólicos comunes. Por la misma razón de ser tantas y tan diversas, no son, en su mayoría, comunes. Voy a citar unas pocas cifras de mi propia experiencia en la Red Colombiana de Medicina Genética -PREGEN- la entidad que creé hace más de 30 años y que se ha encargado de ofrecer el tamizaje neonatal en Colombia y educar a los padres y al cuerpo médico sobre su importancia.
- Published
- 2022
34. UniPROBE, update 2015: new tools and content for the online database of protein-binding microarray data on protein-DNA interactions.
- Author
-
Maxwell A. Hume, Luis A. Barrera, Stephen S. Gisselbrecht, and Martha L. Bulyk
- Published
- 2015
- Full Text
- View/download PDF
35. Implementation of stubble in agricultural production: A review
- Author
-
Luis Ángel Barrera Guzmán, Jorge Cadena-Iñiguez, and Gabriela Ramírez-OjedaGabriela
- Subjects
General Medicine - Abstract
Objective: To elucidate the benefits that agricultural residues or stubble have in agricultural activities, through a bibliographic and objective review that could help to make the actors of agricultural sector aware of the benefits and their correct implementation in production systems. Design/methodology/approach: A detailed review of scientific articles from the main academic databases and repositories was performed. We took into account aspects such as the use of stubble as plant cover; effects and changes in physical and chemical structure of soil; crop yield and use for livestock feeding. Results: The use of stubble has beneficial effects on agricultural activities. In agriculture, they have positive effects for the proliferation of beneficial organisms and assimilation of nutrients, which are easily absorbed by plants. Consequently, crop yield is maximized both in quantity and quality terms. In livestock sector, the stubble implement provides nutrients such as proteins, ashes and vitamins, which together with conventional diets, reduce production costs and improve meat and milk production. Limitations on study/implications: The repertoire of scientific articles related to stubble is very broad, making it difficult to assimilate the information. In Mexico, particularly, more research is needed to inform farmers of the possible uses of stubble. Findings/conclusions: The use of stubble in agricultural activities generates benefits at ecological and economic levels, which ensure the sustainability and resilience of agroecosystems. Nutritional content of stubble is a function of the species and/or varieties of agricultural species. The lack of knowledge in the management of stubble leads to these being incinerated, releasing particles and toxic and polluting substances for the environment, further favoring the conditions for greenhouse effect.
- Published
- 2022
36. Análisis de uma cartera de inversión, basada em los princípios del metodo de inversión em valor (value investing), e inversión focalizada (focus investing): un estudio de caso
- Author
-
Gustavo Alberto Barredo Baqueiro, Luis Martín Barrera Ramírez, and Valentín Alonso Novelo
- Published
- 2022
37. REFLEXIONES SOBRE LA TRANSFORMACION EN LAS PyMES Y LA GENERACION MILENIO EN TIEMPOS DE PANDEMIA
- Author
-
Jorge Ramón Salazar-Cantón, Valentín Alonso-Novelo, and Luis Martín Barrera-Ramírez
- Published
- 2022
38. Large genomic deletions delineate Mycobacterium tuberculosis L4 sublineages in South American countries
- Author
-
Andres Baena, Felipe Cabarcas, Juan C. Ocampo, Luis F. Barrera, and Juan F. Alzate
- Subjects
Multidisciplinary - Abstract
Mycobacterium tuberculosis (Mtb) is still one of the primary pathogens of humans causing tuberculosis (TB) disease. Mtb embraces nine well-defined phylogenetic lineages with biological and geographical disparities. The lineage L4 is the most globally widespread of all lineages and was introduced to America with European colonization. Taking advantage of many genome projects available in public repositories, we undertake an evolutionary and comparative genomic analysis of 522 L4 Latin American Mtb genomes. Initially, we performed careful quality control of public read datasets and applied several thresholds to filter out low-quality data. Using a genome de novo assembly strategy and phylogenomic methods, we spotted novel south American clades that have not been revealed yet. Additionally, we describe genomic deletion profiles of these strains from an evolutionary perspective and report Mycobacterium tuberculosis L4 sublineages signature-like gene deletions, some of the novel. One is a specific deletion of 6.5 kbp that is only present in sublineage 4.1.2.1. This deletion affects a complex group of 10 genes with putative products annotated, among others, as a lipoprotein, transmembrane protein, and toxin/antitoxin system proteins. The second novel deletion spans for 4.9 kbp and specific of a particular clade of the 4.8 sublineage and affects 7 genes. The last novel deletion affects 4 genes, extends for 4.8 kbp., and is specific to some strains within the 4.1.2.1 sublineage that are present in Colombia, Peru and Brasil.
- Published
- 2023
39. Phylogenetic relationships among Mexican species of the genus Sechium (Cucurbitaceae)
- Author
-
Jaime Sahagún-Castellanos, Juan Porfirio Legaria-Solano, Jorge Cadena-Iñiguez, and Luis Ángel Barrera-Guzmán
- Subjects
Phylogenetic tree ,Genus ,Botany ,Plant Science ,Sechium ,Biology ,biology.organism_classification ,Cucurbitaceae - Published
- 2021
40. Distribution and Climatic Adaptation of Wild Tomato (
- Author
-
Gabriela, Ramírez-Ojeda, Juan Enrique, Rodríguez-Pérez, Eduardo, Rodríguez-Guzmán, Jaime, Sahagún-Castellanos, José Luis, Chávez-Servia, Iris E, Peralta, and Luis Ángel, Barrera-Guzmán
- Abstract
Tomato (
- Published
- 2022
41. Sistema Inteligente de Respuesta a Correos Electrónicos.
- Author
-
Luis Carlos Barrera, Luis Fernando Niño, and Andrés Romero
- Published
- 2005
42. Rationally Designed Base Editors for Precise Editing of the Sickle Cell Disease Mutation
- Author
-
Alexander Liquori, Ian Slaymaker, Daisy Lam, Giuseppe Ciaramella, Dieter Lam, Adam J. Hartigan, David A. Born, Jeremy Decker, Fei Ann Ran, S. Haihua Chu, Lo-I Cheng, Holly A. Rees, Jeffrey Marshall, Michael S. Packer, Bob Gantzer, Nicole M. Gaudelli, Jenny Olins, Luis A. Barrera, and Yi Yu
- Subjects
Transition (genetics) ,Computer science ,Cas9 ,Cell ,RNA ,Computational biology ,Base (topology) ,chemistry.chemical_compound ,Protospacer adjacent motif ,medicine.anatomical_structure ,chemistry ,Genetics ,medicine ,DNA ,Biotechnology ,Ribonucleoprotein - Abstract
Base editors are fusions of a deaminase and CRISPR-Cas ribonucleoprotein that allow programmable installment of transition mutations without double-strand DNA break intermediates. The breadth of potential base editing targets is frequently limited by the requirement of a suitably positioned Cas9 protospacer adjacent motif. To address this, we used structures of Cas9 and TadA to design a set of inlaid base editors (IBEs), in which deaminase domains are internal to Cas9. Several of these IBEs exhibit shifted editing windows and greater editing efficiency, enabling editing of targets outside the canonical editing window with reduced DNA and RNA off-target editing frequency. Finally, we show that IBEs enable conversion of the pathogenic sickle cell hemoglobin allele to the naturally occurring HbG-Makassar variant in patient-derived hematopoietic stem cells.
- Published
- 2021
43. Estrategias pedagógicas y motivacionales en los instructores para promover el ingreso y permanencia de aprendices
- Author
-
Luis Ernesto Barrera, Cristina Ruiz Corrales, Katherine Meneses Ramírez, and Iván Mauricio Castañeda Gómez
- Subjects
Training center ,media_common.quotation_subject ,Emotional intelligence ,Service (economics) ,Pedagogy ,Assertiveness ,Empathy ,Augmented reality ,Sociology ,Apprenticeship ,media_common ,Qualitative research - Abstract
Esta investigación pretende analizar los recursos pedagógicos del instructor que ayudan a la motivación de los aprendices en tres centros de formación del SENA (Centros de formación de Comercio y Servicio Regional Atlántico, Regional Antioquia y Centro Agropecuario y de Biotecnología el Porvenir. Regional Córdoba). El estudio se enmarcó en la investigación cualitativa naturalista para lo cual se escogieron 3 aprendices, 3 instructores, 1 coordinador académico y 3 líderes de bienestar del aprendiz en cada centro de formación del SENA. Los hallazgos significativos son tendientes a las necesidades de: Acompañamiento Docente, Actividades lúdicas, Dinámico, Off line, Imágenes y videos, Fácil acceso, Aprendiz amigo, Empatía, Recompensa, Realidad aumentada, 3D, multimedia, video juegos, Seguimiento al desarrollo integral, Comunicación asertiva, Inteligencia emocional.
- Published
- 2021
44. EDAPHOCLIMATIC DIVERSITY AND ECOLOGICAL DESCRIPTORS OF Guadua BAMBOO SPECIES (Poaceae: Bambusoideae) IN MEXICO
- Author
-
Luis Ángel Barrera-Guzmán, Gabriela Orozco-Gutiérrez, Gabriela Ramírez-Ojeda, and Eduardo RuizSanchez
- Subjects
Bamboo ,Guadua ,Ecology ,media_common.quotation_subject ,Poaceae ,Biology ,Bambusoideae ,biology.organism_classification ,Diversity (politics) ,media_common - Published
- 2021
45. A Review of History, Production and Storage of Hydrogen
- Author
-
John-William Grimaldo-Guerrero, Juan De la Hoz Barcelo, Daniel Rivera-Pacheco, Luis Ramos-Barrera, and Ubaldo Martinez-Palacio
- Subjects
Renewable energy ,Hydrogen production ,General Engineering ,Hydrogen storage - Abstract
The need to reduce fossil fuel consumption has created opportunities for alternative fuels, including the migration of hydrogen as an unconventional alternative fuel. This alternative has more significant environmental and energy benefits due to the acquisition of raw materials and the integration of renewable energy sources. The research presents a review of historical evolution, a bibliometric analysis, and the processes used to produce and store this molecule. The POx and pyrolysis processes have the highest amount of research. At the same time, electrolysis is the process that has had the most significant growth, and research indicates that they allow greater sustainability due to the integration of renewable energies. Research trends indicate studies for integrating renewable energy resources and materials to improve chemical properties to increase capacity storage and decrease the risks due to high volatility.
- Published
- 2021
46. Errores innatos en el metabolismo: Un abordaje integral del diagnóstico al tratamiento
- Author
-
Luis Alejandro Barrera Avellaneda, Angela Johana Espejo Mojica, Eugenia Espinosa García, Olga Yaneth Echeverri Peña and Luis Alejandro Barrera Avellaneda, Angela Johana Espejo Mojica, Eugenia Espinosa García, Olga Yaneth Echeverri Peña
- Published
- 2014
47. Lymph node tissue thyroglobulin washout measurement in metastatic papillary thyroid carcinoma
- Author
-
Jose A. Hakim-Tawil, Viviana Barrera-Penagos, Luis E. Barrera-Herrera, Javier A Romero-Enciso, Humberto Rivera-Gonzalez, Mauricio A. Palau-Lazaro, Deyanira González-Devia, and Paula A Rodriguez-Urrego
- Subjects
endocrine system ,medicine.medical_specialty ,Thyroglobulin Measurement ,medicine.diagnostic_test ,business.industry ,medicine.medical_treatment ,Thyroid ,Thyroidectomy ,030209 endocrinology & metabolism ,Thyroid carcinoma ,03 medical and health sciences ,0302 clinical medicine ,Fine-needle aspiration ,medicine.anatomical_structure ,Cytopathology ,030220 oncology & carcinogenesis ,Biopsy ,medicine ,Thyroglobulin ,Radiology ,business - Abstract
Aims and Background: Papillary thyroid carcinoma is the most frequent subtype of thyroid carcinoma and usually has a favorable prognosis, however, locoregional metastases are reported in approximately 5-20% of the cases. Literature reports that when using a combination of ultrasound, fine needle aspiration and tissue thyroglobulin levels to diagnose metastases, sensitivity and specificity radically increase. The aim of this study was to validate tissue thyroglobulin measurement in local patients and compare these results to radiological and histopathological diagnosis. Materials and Methods: A cross-sectional study was carried out to measure tissue thyroglobulin levels in lymph nodes obtained by fine needle aspiration and/or biopsy from patients with adenopathy suspected of metastatic papillary thyroid carcinoma. Tissue thyroglobulin was measured by chemiluminescence and was considered positive for metastatic papillary thyroid carcinoma when levels were ?1.7 ng / mL in patients with a history of thyroidectomy and > 36 ng/mL in patients without a history of thyroidectomy (4). Results: Our results confirm that the measurement of TTG by chemiluminescence can be made from the same tissue sample, giving the patient and the clinician greater diagnostic certainty. Conclusions: TTG measurement through chemiluminescence is a low-cost technique that can improve diagnostic performance of pathological analysis. Levels can be measured from the same needle used in cytology/biopsy providing both the patient and clinician greater diagnostic certainty, increasing the diagnostic sensitivity and specificity of the procedure without the need for further invasive interventions. Key Message: TTG measurement through chemiluminescence is a low-cost technique that can improve diagnostic performance of pathological analysis. Keywords: Papillary thyroid carcinoma thyroglobulin local lymph node assay, Neoplasm metastasis.
- Published
- 2020
48. Estudio técnico-económico para identificar áreas con potencial para producir piña en el trópico húmedo de México
- Author
-
Alejandra Vélez-Izquierdo, José Luis Jolalpa-Barrera, Jaime Rangel-Quintos, José Antonio Espinosa-García, Diana Uresti-Durán, and Jesús Uresti Gil
- Subjects
Crop ,Biomass (ecology) ,Geography ,Yield (wine) ,Forestry ,General Medicine ,Pineapple (Fruit) ,Agroecology ,Humid tropics ,Economic potential - Abstract
Con el objetivo de identificar el potencial productivo y económico del cultivo de piña a nivel de unidad de respuesta hidrológica, cuenca y estado, considerando el rendimiento y la relación beneficio costo (R B/C), se realizó un estudio técnico-económico, en los principales estados productores del Trópico Húmedo de México. Para ello se simuló y cartografió el rendimiento potencial de biomasa aérea total y fruta de piña en nueve estados del sur-sureste de México y se identificaron las áreas con mayor potencial para el cultivo. Asimismo, se estimaron los costos de establecimiento, de mantenimiento e ingresos del paquete tecnológico de malla sombra y se evaluó la rentabilidad financiera del cultivo para cada región identificada. Los resultados muestran que el cultivo de piña es rentable cuando se produce más de 45.8 t ha-1. Se identificaron 596 666 ha con potencial para producir piña, ubicadas en Campeche, Chiapas, Oaxaca, Puebla, Quintana Roo, Tabasco, Veracruz y Yucatán, siendo el estado de Campeche el que presentó el mayor rendimiento (90 t ha-1) y una R B/C de 1.88. Se concluye que la producción de piña es rentable en aquellas regiones que presentan condiciones agroecológicas idóneas para su producción.
- Published
- 2020
49. POTENTIAL DISTRIBUTION OF DOMESTICATED Sechium edule (CUCURBITACEAE) IN MEXICO
- Author
-
Luis Ángel Barrera Guzmán, Jorge Cadena Iñiguez, Juan Porfirio Legaria Solano, Jaime Sahagún Castellanos, and Gabriela Ramírez Ojeda
- Subjects
General Agricultural and Biological Sciences - Abstract
Mexico is the centre of origin of the chayote (Sechium edule (Jacq.) Sw), an important plant in human consumption and in pharmaceuticals. The objective of this study was to determine the potential distribution of domesticated S. edule in Mexico using seven species distribution algorithms, to efficiently manage S. edule resources and help its conservation by identifying patterns of geographic distribution. Otherwise, areas of high suitability can be used to produce improved seed at a lower cost. 162 GBIF occurrence points and nine layers in raster format were used to evaluate seven algorithms of species distribution models. To evaluate the reliability and performance of the models, the statistics Area Under the Curve (AUC) and true skill statistic was used. Predominant climate types were Cwb (33.3 %) and Aw (17.9 %); predominant soil types were leptosol (33.3 %) and phaozem (16.7 %). The seven models showed areas of high suitability (> 0.75) in Chiapas, Guerrero, Oaxaca, Veracruz, Tabasco, Puebla and Hidalgo states. AUC values for the seven models were > 0.8 and their performance was adequate (0.4 > TSS < 0.7). Classification tree analysis was found to be the best algorithm measured by AUC (0.90); however, the seven models were adequate to explain S. edule distribution in Mexico. S. edule climatic adaptability also allows to be distributed towards the Yucatan Peninsula and western Mexico. The distribution of S. edule in Mexico, according to the studied algorithms, is limited to total annual precipitation and temperature seasonality.
- Published
- 2022
50. Acquired FXI deficiency in a systemic lupus erythematosus patient: Evolution and coagulation phenotypic changed to lupus anticoagulant during immunosuppressive therapy
- Author
-
Marta Martinuzzo, Cristian Maximiliano Seehaus, Marina Sol López, Luis Horacio Barrera, Lendro Ferreyra Garrott, Paulina Quiñones Maffassanti, Verónica Privitera, Fernando Chuliber, Diana Penchasky, Estela S. Viñuales, and Jorge Arbelbide
- Subjects
Immunosuppression Therapy ,Lupus Coagulation Inhibitor ,Biochemistry (medical) ,Clinical Biochemistry ,Humans ,Lupus Erythematosus, Systemic ,Hematology ,General Medicine ,Antiphospholipid Syndrome ,Blood Coagulation ,Blood Coagulation Factors - Published
- 2022
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.