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2,354 results on '"Ludwig-Maximilian University of Munich"'

1. DER KLIMAWANDEL

Author: Ludwig Maximilian University of Munich, Lesch, Harald, Theis-Bröhl, Katharina, Bröhl-August, Judith, Lesch, Harald, Theis-Bröhl, Katharina, and Bröhl-August, Judith
Published: 2021
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2. WAS KANN ICH TUN?

Author: Ludwig Maximilian University of Munich, Lesch, Harald, Theis-Bröhl, Katharina, Bröhl-August, Judith, Lesch, Harald, Theis-Bröhl, Katharina, and Bröhl-August, Judith
Published: 2021
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3. ZUKUNFTSBLICK

Author: Ludwig Maximilian University of Munich, Lesch, Harald, Theis-Bröhl, Katharina, Bröhl-August, Judith, Lesch, Harald, Theis-Bröhl, Katharina, and Bröhl-August, Judith
Published: 2021
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4. AUSWIRKUNGEN DES KLIMAWANDELS

Author: Ludwig Maximilian University of Munich, Lesch, Harald, Theis-Bröhl, Katharina, Bröhl-August, Judith, Lesch, Harald, Theis-Bröhl, Katharina, and Bröhl-August, Judith
Published: 2021
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5. DAS KLIMASYSTEM DER ERDE

Author: Ludwig Maximilian University of Munich, Lesch, Harald, Theis-Bröhl, Katharina, Bröhl-August, Judith, Lesch, Harald, Theis-Bröhl, Katharina, and Bröhl-August, Judith
Published: 2021
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6. DEN TREIBHAUSEFFEKT VERSTEHEN

Author: Ludwig Maximilian University of Munich, Lesch, Harald, Theis-Bröhl, Katharina, Bröhl-August, Judith, Lesch, Harald, Theis-Bröhl, Katharina, and Bröhl-August, Judith
Published: 2021
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7. WIE BESONDERS IST DIE ERDE?

Author: Ludwig Maximilian University of Munich, Lesch, Harald, Theis-Bröhl, Katharina, Bröhl-August, Judith, Lesch, Harald, Theis-Bröhl, Katharina, and Bröhl-August, Judith
Published: 2021
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8. Dipolarization Fronts in the Jovian Magnetotail: Statistical Survey of Ion Intensity Variations Using Juno Observations

Author: Blöcker, A., Kronberg, E. A., Grigorenko, E. E., Roussos, E., Clark, G., 1 Department of Earth and Environmental Sciences Ludwig Maximilian University of Munich Munich Germany, 2 Space Research Institute Russian Academy of Sciences Moscow Russia, 3 Max Planck Institute for Solar System Research Göttingen Germany, and 4 Johns Hopkins University Applied Physics Laboratory Laurel MD USA
Subjects: Juno, Geophysics, ddc:523, Space and Planetary Science, Jovian magnetotail, dipolarization fronts, JEDI, energetic ions
Abstract: Energetic particle acceleration and energization in planetary magnetotails are often associated with dipolarization fronts characterized by a rapid increase of the meridional component of the magnetic field. Despite many studies of dipolarization events in Earth's magnetotail, Jupiter’s magnetotail provides an almost ideal environment to study high‐energetic ion acceleration by dipolarization fronts because of its large spatial scales and plasma composition of heavy and light ions. In this study, we focus on the response of different high‐energetic ion intensities (H, He, S, and O) to prominent magnetic dipolarization fronts inside the Jovian magnetotail. We investigate if ion energization and acceleration are present in the observations around the identified dipolarization fronts. Therefore, we present a statistical study of 87 dipolarization front signatures, which are identified in the magnetometer data of the Juno spacecraft from July 2016 to July 2021. For the ion intensity analysis, we use the energetic particle observations from the Jupiter Energetic Particle Detector Instrument. Our statistical study reveals that less than half of the identified events are accompanied by an increase of the ion intensities, while most of the other events show no significant change in the ion intensity dynamics. In about 40% of the events located in the dawn sector a significant decrease of the energy spectral index is detected indicating ion acceleration by the dipolarization fronts., Key Points: Eighty‐seven prominent dipolarization front signatures are observed in the MAG data during Juno's prime mission during 21:00–05:30 local time. Less than half of the identified events are accompanied by an increase of the ion intensities. In 40% of the events observed on the dawn side a significant decrease of the energy spectral index indicates ion acceleration by the fronts., Volkswagen Foundation http://dx.doi.org/10.13039/501100001663, Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659, https://doi.org/10.17189/1519711, https://doi.org/10.17189/1519713
Published: 2023

9. Greigite (Fe3S4) Formation in Artificial Sediments via Solid‐State Transformation of Lepidocrocite

Author: Roud, Sophie C., Gilder, Stuart A., Park, SoHyun, and 1 Department of Earth and Environmental Sciences Ludwig Maximilian University of Munich Munich Germany
Subjects: Geophysics, Geochemistry and Petrology, ddc:549
Abstract: Greigite (Fe3S4) is a ferrimagnetic iron‐sulfide mineral that forms in sediments during diagenesis. Greigite growth can occur diachronously within a stratigraphic profile, complicating or overprinting environmental and paleomagnetic records. An important objective for paleo‐ and rock‐magnetic studies is to identify the presence of greigite and to discern its formation conditions. Greigite detection remains, however, challenging and its magnetic properties obscure due to the lack of pure, stable material of well‐defined grain size. To overcome these limitations, we report a new method to selectively transform lepidocrocite to greigite via the intermediate phase mackinawite (FeS). In‐situ magnetic characterization was performed on discrete samples with different sediment substrates. Susceptibility and chemical remanent magnetization increased proportionally over time, defining two distinct greigite growth regimes. Temperature dependent and constant initial growth rates indicate a solid‐state FeS to greigite transformation with an activation energy of 78–90 kJ/mol. Low and room temperature magnetic remanence and coercivity ratios match with calculated mixing curves for superparamagnetic (SP) and single domain (SD) greigite and suggest ∼25% and ∼50% SD proportions at 300 and 100 K, respectively. The mixing trend coincides with empirical data reported for natural greigite‐bearing sediments, suggesting a common SP endmember size of 5–10 nm that is likely inherited from mackinawite crystallites. The average particle size of 20–50 nm determined by X‐ray powder diffraction and electron microscopy accords with theoretical predictions of the SP/SD threshold size in greigite. The method constitutes a novel approach to synthesize greigite and to investigate its formation in sediments., Plain Language Summary: Sediments provide continuous records of Earth's ancient magnetic field, which lend insights into the workings of the geodynamo and help to establish the geologic time scale through global magnetostratigraphic correlation. Greigite is a magnetic iron sulfide mineral that commonly forms after deposition, thereby remagnetizing the sediment and complicating interpretation of the magnetic record. Understanding greigite formation and detecting its presence is fundamental for obtaining reliable records of the paleomagnetic field, yet knowledge of how greigite grows and how its magnetic properties evolve during growth remains limited. This article outlines a novel approach to form greigite in sediments and to monitor its growth kinetics, grain size and magnetic remanence acquisition. The magnetic properties of the synthetic sediments resemble those of natural greigite‐bearing sediments and match well with theoretical calculations, which can help quantify grain sizes in sedimentary greigite. The reported method and our results contribute to a better understanding of greigite formation and chemical magnetic remanence acquisition in sediments., Key Points: We present a new method to grow greigite in aqueous sediments and create a chemical remanent magnetization under controlled conditions. Greigite grain sizes of 20–50 nm span the superparamagnetic to single domain threshold, consistent with theoretical predictions. Our experimental hysteresis data coincide with calculated mixing curves allowing better quantification of greigite particle sizes in nature., Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659, https://doi.org/10.5281/zenodo.6521653
Published: 2022

10. CXCR6 deficiency impairs cancer vaccine efficacy and CD8+ resident memory T-cell recruitment in head and neck and lung tumors

Author: Karaki, Soumaya, Blanc, Charlotte, Tran, Thi, Galy-Fauroux, Isabelle, Mougel, Alice, Dransart, Estelle, Anson, Marie, Tanchot, Corinne, Paolini, Lea, Gruel, Nadege, Gibault, Laure, Lepimpec-Barhes, Francoise, Fabre, Elizabeth, Benhamouda, Nadine, Badoual, Cecile, Damotte, Diane, Donnadieu, Emmanuel, Kobold, Sebastian, Mami-Chouaib, Fathia, Golub, Rachel, Johannes, Ludger, Tartour, Eric, Vougny, Marie-Christine, Université Sorbonne Paris Cité - - USPC2011 - ANR-11-IDEX-0005 - IDEX - VALID, Paris-Centre de Recherche Cardiovasculaire (PARCC (UMR_S 970/ U970)), Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Equipe labellisée Ligue contre le Cancer, Chimie biologique des membranes et ciblage thérapeutique (CBMCT - UMR 3666 / U1143), Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Unité de génétique et biologie des cancers (U830), Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Département de Recherche Translationnelle, Institut Curie [Paris], Service d’anatomo‑pathologie [AP-HP Hôpital Européen Georges Pompidou] (Centre de Ressources biologiques), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Centre de Recherche des Cordeliers (CRC (UMR_S_1138 / U1138)), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Université Paris Cité (UPCité), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut Cochin (IC UM3 (UMR 8104 / U1016)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Center for Integrated Protein Science (CIPSM), Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM)-Ludwig-Maximilians-Universität München (LMU)-Helmholtz Zentrum München = German Research Center for Environmental Health, German Center for Lung Research - DZL [Munich, Germany], Immunologie intégrative des tumeurs et immunothérapie des cancers (INTIM), Institut Gustave Roussy (IGR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay, Lymphopoïèse (Lymphopoïèse (UMR_1223 / U1223 / U-Pasteur_4)), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), This work has been funded by Fondation ARC, INCA PLBio, Labex Immuno-Oncology, Site intégré de recherche en cancérologie (SIRIC CARPEM, SIRIC Curie), Cancéropole d’Ile de France, Carnot Curie Cancer, FONCER. SK is supported by the European Research Council (grant 756017, ARMOR-T)., ANR-11-IDEX-0005,USPC,Université Sorbonne Paris Cité(2011), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC), Département de recherche translationnelle, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Université de Paris (UP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM)-Helmholtz-Zentrum München (HZM)-Ludwig Maximilian University of Munich [Germany] (LMU München), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), École Pratique des Hautes Études (EPHE), and Technical University of Munich (TUM)-Ludwig Maximilian University of Munich [Germany] (LMU München)-Helmholtz-Zentrum München (HZM)
Subjects: Lung Neoplasms, [SDV.IMM] Life Sciences [q-bio]/Immunology, Vaccine Efficacy, immunization, Cancer Vaccines, Memory T Cells, Lymphocytes, Tumor-Infiltrating, Cell Line, Tumor, Tumor Microenvironment, Animals, Humans, Administration, Intranasal, RC254-282, Receptors, CXCR6, Mice, Knockout, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Basic Tumor Immunology, Chemokine CXCL16, adaptive immunity, vaccination, Tumor Burden, Mice, Inbred C57BL, Phenotype, Head and Neck Neoplasms, CD8-positive T-lymphocytes, [SDV.IMM]Life Sciences [q-bio]/Immunology, Female, Immunologic Memory
Abstract: International audience; Background Resident memory T lymphocytes (TRM) are located in tissues and play an important role in immunosurveillance against tumors. The presence of TRM prior to treatment or their induction is associated to the response to anti-Programmed cell death protein 1 (PD-1)/Programmed death-ligand 1 (PD-L1) immunotherapy and the efficacy of cancer vaccines. Previous work by our group and others has shown that the intranasal route of vaccination allows more efficient induction of these cells in head and neck and lung mucosa, resulting in better tumor protection. The mechanisms of in vivo migration of these cells remains largely unknown, apart from the fact that they express the chemokine receptor CXCR6.Methods We used CXCR6-deficient mice and an intranasal tumor vaccination model targeting the Human Papillomavirus (HPV) E7 protein expressed by the TC-1 lung cancer epithelial cell line. The role of CXCR6 and its ligand, CXCL16, was analyzed using multiparametric cytometric techniques and Luminex assays.Human biopsies obtained from patients with lung cancer were also included in this study.Results We showed that CXCR6 was preferentially expressed by CD8+ TRM after vaccination in mice and also on intratumoral CD8+ TRM derived from human lung cancer. We also demonstrate that vaccination of Cxcr6-deficient mice induces a defect in the lung recruitment of antigen-specific CD8+ T cells, preferentially in the TRM subsets. In addition, we found that intranasal vaccination with a cancer vaccine is less effective in these Cxcr6-deficient mice compared with wild-type mice, and this loss of efficacy is associated with decreased recruitment of local antitumor CD8+ TRM. Interestingly, intranasal, but not intramuscular vaccination induced higher and more sustained concentrations of CXCL16, compared with other chemokines, in the bronchoalveolar lavage fluid and pulmonary parenchyma.Conclusions This work demonstrates the in vivo role of CXCR6-CXCL16 axis in the migration of CD8+ resident memory T cells in lung mucosa after vaccination, resulting in the control of tumor growth. This work reinforces and explains why the intranasal route of vaccination is the most appropriate strategy for inducing these cells in the head and neck and pulmonary mucosa, which remains a major objective to overcome resistance to anti-PD-1/PD-L1, especially in cold tumors.
Published: 2021

11. Extracellular LGALS3BP regulates neural progenitor position and relates to human cortical complexity

Author: Zhisong He, Matteo Lenge, Laure Coquand, Pierpaolo D' Andrea, Stephan A. Sieber, Adam C. O’Neill, Davide Mei, Silvia Cappello, Christina Kyrousi, Rossella Di Giaimo, Stephen P. Robertson, Alexandre D Baffet, Isabel Y. Buchsbaum, Andrea Forero Echeverry, Guimiot Fabien, Cristiana Cruceanu, Pavel Kielkowski, Agnieska Brazovskaja, Elisabeth B. Binder, Barbara Treutlein, Renzo Guerrini, Alexander Belka, Shahryar Khattak, Frances Elmslie, Max Planck Institute of Psychiatry, Max-Planck-Gesellschaft, National and Kapodistrian University of Athens (NKUA), University of Otago [Dunedin, Nouvelle-Zélande], Max Planck Institute for Evolutionary Anthropology [Leipzig], Department of Biosystems Science and Engineering [ETH Zürich] (D-BSSE), Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich), Center for Integrated Protein Science (CIPSM), Technical University of Munich (TUM)-Helmholtz-Zentrum München (HZM)-Ludwig Maximilian University of Munich [Germany] (LMU München), Ludwig-Maximilians-Universität München (LMU), Optimisation thérapeutique en Neuropsychopharmacologie (OPTeN (UMR_S_1144 / U1144)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), Institut Curie [Paris], University of Naples Federico II, Università degli Studi di Firenze = University of Florence [Firenze] (UNIFI), Technische Universität Dresden = Dresden University of Technology (TU Dresden), Royal College of Surgeons in Ireland (RCSI), Hôpital Robert Debré, University of London [London], Kyrousi, Christina, O'Neill, Adam C, Brazovskaja, Agnieska, He, Zhisong, Kielkowski, Pavel, Coquand, Laure, Di Giaimo, Rossella, D' Andrea, Pierpaolo, Belka, Alexander, Forero Echeverry, Andrea, Mei, Davide, Lenge, Matteo, Cruceanu, Cristiana, Buchsbaum, Isabel Y, Khattak, Shahryar, Fabien, Guimiot, Binder, Elisabeth, Elmslie, France, Guerrini, Renzo, Baffet, Alexandre D, Sieber, Stephan A, Treutlein, Barbara, Robertson, Stephen P, Cappello, Silvia, and Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM)-Helmholtz-Zentrum München (HZM)-Ludwig Maximilian University of Munich [Germany] (LMU München)
Subjects: [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, General Physics and Astronomy, Neocortex, Induced Pluripotent Stem Cell, Extracellular matrix, Mice, 0302 clinical medicine, Lateral Ventricle, Lateral Ventricles, Neural Stem Cell, Gyrification, [SDV.BDD]Life Sciences [q-bio]/Development Biology, Cerebral Cortex, 0303 health sciences, Multidisciplinary, Neurodevelopmental disorders, Cell Differentiation, Human brain, Cell biology, ddc, Corticogenesis, medicine.anatomical_structure, Models, Animal, Female, Neuroglia, Human, Extracellular Vesicle, Science, Induced Pluripotent Stem Cells, Subventricular zone, Biology, General Biochemistry, Genetics and Molecular Biology, Article, 03 medical and health sciences, Extracellular Vesicles, Antigens, Neoplasm, Extracellular, medicine, Biomarkers, Tumor, Animals, Humans, Progenitor cell, 030304 developmental biology, Progenitor, Neural stem cells, Development of the nervous system, Neurological disorders, Animal, General Chemistry, Mice, Inbred C57BL, 030217 neurology & neurosurgery
Abstract: Basal progenitors (BPs), including intermediate progenitors and basal radial glia, are generated from apical radial glia and are enriched in gyrencephalic species like humans, contributing to neuronal expansion. Shortly after generation, BPs delaminate towards the subventricular zone, where they further proliferate before differentiation. Gene expression alterations involved in BP delamination and function in humans are poorly understood. Here, we study the role of LGALS3BP, so far known as a cancer biomarker, which is a secreted protein enriched in human neural progenitors (NPCs). We show that individuals with LGALS3BP de novo variants exhibit altered local gyrification, sulcal depth, surface area and thickness in their cortex. Additionally, using cerebral organoids, human fetal tissues and mice, we show that LGALS3BP regulates the position of NPCs. Single-cell RNA-sequencing and proteomics reveal that LGALS3BP-mediated mechanisms involve the extracellular matrix in NPCs’ anchoring and migration within the human brain. We propose that its temporal expression influences NPCs’ delamination, corticogenesis and gyrification extrinsically., Nature Communications, 12, ISSN:2041-1723
Published: 2021

12. JASPer controls interphase histone H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila

Author: Tom W. Muir, Peter B. Becker, Sylvain Maenner, Jørgen Johansen, Yeran Li, Catherine Regnard, Jack Girton, Kristen M. Johansen, Felix Wojcik, Tamas Schauer, Christian Albig, Geoffrey P. Dann, Weili Cai, Silke Krause, Chao Wang, Center for Integrated Protein Science (CIPSM), Technical University of Munich (TUM)-Helmholtz-Zentrum München (HZM)-Ludwig Maximilian University of Munich [Germany] (LMU München), Roy J Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University (ISU), Southwest Jiaotong University (SWJTU), Perelman School of Medicine, University of Pennsylvania [Philadelphia], Princeton University, Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM)-Ludwig Maximilian University of Munich [Germany] (LMU München)-Helmholtz-Zentrum München (HZM), and Centre National de la Recherche Scientifique (CNRS)-Université de Lorraine (UL)
Subjects: 0301 basic medicine, Heterochromatin, Science, [SDV]Life Sciences [q-bio], General Physics and Astronomy, Kinases, Protein Serine-Threonine Kinases, Methylation, Article, Chromosomes, General Biochemistry, Genetics and Molecular Biology, Cell Line, Histones, 03 medical and health sciences, 0302 clinical medicine, Histone post-translational modifications, Animals, Drosophila Proteins, Humans, Nucleosome, Phosphorylation, lcsh:Science, Interphase, Gene, 030304 developmental biology, 0303 health sciences, Multidisciplinary, biology, Kinase, Chemistry, General Chemistry, Chromatin Assembly and Disassembly, Chromatin, Nucleosomes, Cell biology, Drosophila melanogaster, 030104 developmental biology, Histone, biology.protein, lcsh:Q, Heterochromatin protein 1, Protein Processing, Post-Translational, 030217 neurology & neurosurgery, Post-translational modifications
Abstract: In flies, the chromosomal kinase JIL-1 is responsible for most interphase histone H3S10 phosphorylation and has been proposed to protect active chromatin from acquiring heterochromatic marks, such as dimethylated histone H3K9 (H3K9me2) and HP1. Here, we show that JIL-1’s targeting to chromatin depends on a PWWP domain-containing protein JASPer (JIL-1 Anchoring and Stabilizing Protein). JASPer-JIL-1 (JJ)-complex is the major form of kinase in vivo and is targeted to active genes and telomeric transposons via binding of the PWWP domain of JASPer to H3K36me3 nucleosomes, to modulate transcriptional output. JIL-1 and JJ-complex depletion in cycling cells lead to small changes in H3K9me2 distribution at active genes and telomeric transposons. Finally, we identify interactors of the endogenous JJ-complex and propose that JIL-1 not only prevents heterochromatin formation but also coordinates chromatin-based regulation in the transcribed part of the genome., The chromosomal kinase JIL-1 is responsible for interphase histone H3S10 phosphorylation and has been proposed to protect active chromatin from heterochromatinisation. Here, the authors show that JIL-1 is stabilized and anchored to active genes and telomeric transposons by JASPer, which binds to H3K36me3 nucleosomes via its PWWP domain.
Published: 2019

13. Conformational plasticity of the VEEV macro domain is important for binding of ADP-ribose

Author: Dioni Ntonti, Maria Birkou, Bruno Canard, Bruno Coutard, Minos-Timotheos Matsoukas, Garyfallia I. Makrynitsa, Konstantinos D. Marousis, Nicolas Papageorgiou, Georgios A. Spyroulias, Sam Asami, Detlef Bentrop, Department of Pharmacy [Patras], University of Patras, Center for Integrated Protein Science (CIPSM), Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM)-Ludwig-Maximilians-Universität München (LMU)-Helmholtz Zentrum München = German Research Center for Environmental Health, Institute of Physiology II [Freiburg, Germany] (Faculty of Medicine), University of Freiburg [Freiburg], Architecture et fonction des macromolécules biologiques (AFMB), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Unité des Virus Emergents (UVE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), CCSD, Accord Elsevier, University of Patras [Patras], Munich Center for Integrated Protein Science [Garching, Germany] (Department Chemie), Technische Universität München [München] (TUM), Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM)-Ludwig Maximilian University of Munich [Germany] (LMU München)-Helmholtz-Zentrum München (HZM), Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA), and Technical University of Munich (TUM)-Helmholtz-Zentrum München (HZM)-Ludwig Maximilian University of Munich [Germany] (LMU München)
Subjects: Magnetic Resonance Spectroscopy, Viral protein, [SDV.BBM.BS] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], Molecular Conformation, Sequence (biology), Alphavirus, Molecular Dynamics Simulation, Viral Nonstructural Proteins, Virus Replication, medicine.disease_cause, Genome, Article, Encephalitis Virus, Venezuelan Equine, Conformational dynamics, 03 medical and health sciences, chemistry.chemical_compound, Macro domain, NMR spectroscopy, Protein Domains, Structural Biology, Ribose, medicine, Animals, Humans, Horses, ComputingMethodologies_COMPUTERGRAPHICS, 030304 developmental biology, [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Adenosine Diphosphate Ribose, 0303 health sciences, biology, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], 030302 biochemistry & molecular biology, Normal mode analysis, biology.organism_classification, Viral macro domain, 3. Good health, Cell biology, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], chemistry, Viral replication, Venezuelan equine encephalitis virus, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, 15N relaxation, ADP-ribose, Protein Binding
Abstract: Graphical abstract, Highlights • ADPr’s binding triggers conformational changes to the whole VEEV macro domain. • High flexibility of the loops β5-α3 and α3-β6 assist the ADPr’s binding. • Loops around ADPr site undergo a transition pathway between apo and complex state., Venezuelan equine encephalitis virus (VEEV) is a new world alphavirus which can be involved in several central nervous system disorders such as encephalitis and meningitis. The VEEV genome codes for 4 non-structural proteins (nsP), of which nsP3 contains a Macro domain. Macro domains (MD) can be found as stand-alone proteins or embedded within larger proteins in viruses, bacteria and eukaryotes. Their most common feature is the binding of ADP-ribose (ADPr), while several macro domains act as ribosylation writers, erasers or readers. Alphavirus MD erase ribosylation but their precise contribution in viral replication is still under investigation. NMR-driven titration experiments of ADPr in solution with the VEEV macro domain (in apo- and complex state) show that it adopts a suitable conformation for ADPr binding. Specific experiments indicate that the flexibility of the loops β5-α3 and α3-β6 is critical for formation of the complex and assists a wrapping mechanism for ADPr binding. Furthermore, along with this sequence of events, the VEEV MD undergoes a conformational exchange process between the apo state and a low-populated “dark” conformational state.
Published: 2019

14. SETDB1-dependent heterochromatin stimulates alternative lengthening of telomeres

Author: Nehmé Saksouk, Raymund J. Wellinger, Axel Imhof, Mathilde Gauchier, Reini F. Luco, Satoru Ide, Sandrine Sauzet, Serge Urbach, Sophie Kan, Jérôme Déjardin, Amandine Barral, Erin Bonnell, Teresa K. Barth, Eneritz Agirre, Biologie intégrative des organismes marins (BIOM), Observatoire océanologique de Banyuls (OOB), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut de génétique humaine (IGH), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Center for Integrated Protein Science (CIPSM), Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM)-Ludwig Maximilian University of Munich [Germany] (LMU München)-Helmholtz-Zentrum München (HZM), and Technical University of Munich (TUM)-Helmholtz-Zentrum München (HZM)-Ludwig Maximilian University of Munich [Germany] (LMU München)
Subjects: X-linked Nuclear Protein, Heterochromatin, [SDV]Life Sciences [q-bio], Telomeric heterochromatin, Biology, digestive system, 03 medical and health sciences, Mice, 0302 clinical medicine, RNA interference, Cell Line, Tumor, Animals, Humans, Histone Chaperones, Telomeric Repeat Binding Protein 2, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, RNA, Small Interfering, Telomere Shortening, Research Articles, 030304 developmental biology, Cancer, 0303 health sciences, Multidisciplinary, fungi, RNA, SciAdv r-articles, Life Sciences, Mouse Embryonic Stem Cells, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Histone-Lysine N-Methyltransferase, Methyltransferases, Telomere, digestive system diseases, Cell biology, Mice, Inbred C57BL, Repressor Proteins, Cell culture, 030220 oncology & carcinogenesis, Histone methyltransferase, Cancer cell, RNA Interference, Research Article
Abstract: Atypical heterochromatin forms on telomeres and correlates with ALT activities., Alternative lengthening of telomeres, or ALT, is a recombination-based process that maintains telomeres to render some cancer cells immortal. The prevailing view is that ALT is inhibited by heterochromatin because heterochromatin prevents recombination. To test this model, we used telomere-specific quantitative proteomics on cells with heterochromatin deficiencies. In contrast to expectations, we found that ALT does not result from a lack of heterochromatin; rather, ALT is a consequence of heterochromatin formation at telomeres, which is seeded by the histone methyltransferase SETDB1. Heterochromatin stimulates transcriptional elongation at telomeres together with the recruitment of recombination factors, while disrupting heterochromatin had the opposite effect. Consistently, loss of SETDB1, disrupts telomeric heterochromatin and abrogates ALT. Thus, inhibiting telomeric heterochromatin formation in ALT cells might offer a new therapeutic approach to cancer treatment.
Published: 2019
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15. The placebo effect of human augmentation : Anticipating cognitive augmentation increases risk-taking behavior

Author: Steeven Villa, Thomas Kosch, Felix Grelka, Albrecht Schmidt, Robin Welsch, Ludwig Maximilian University of Munich, Humboldt-Universität zu Berlin, Department of Computer Science, Aalto-yliopisto, and Aalto University
Subjects: Human-Computer Interaction, Arts and Humanities (miscellaneous), Placebo effect, Electroencephalography, Human augmentation, User studies, User expectations, General Psychology
Abstract: Funding Information: This work was partly funded by the European Research Council (ERC AMPLIFY, no. 683008), and the Federal Ministry of Education and Research of Germany (BMBF Hive, no. 16SV8183). The German Psychological Society (DGPs) and the local institutional ethics board provided ethical approval for the study: Ethical assessment ID: EK-MIS-2020-023. Human Augmentation Technologies improve human capabilities using technology. In this study, we investigate the placebo effect of Augmentation Technologies. Thirty naïve participants were told to be augmented with a cognitive augmentation technology or no augmentation system while conducting a Columbia Card Task. In this risk-taking measure, participants flip win and loss cards. The sham augmentation system consisted of a brain–computer interface allegedly coordinated to play non-audible sounds that increase cognitive functions. However, no sounds were played throughout all conditions. We show a placebo effect in human augmentation, where a sustained belief of improvement remains after using the sham system and an increase in risk-taking conditional on heightened expectancy using Bayesian statistical modeling. Furthermore, we identify differences in event-related potentials in the electroencephalogram that occur during the sham condition when flipping loss cards. Finally, we integrate our findings into theories of human augmentation and discuss implications for the future assessment of augmentation technologies.
Published: 2023

16. Chromosome topology guides the Drosophila Dosage Compensation Complex for target gene activation

Author: Peter B. Becker, Christian Albig, Yad Ghavi-Helm, Giacomo Cavalli, Tom Sexton, Tamas Schauer, Eileen E. M. Furlong, Catherine Regnard, Center for Integrated Protein Science (CIPSM), Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM)-Ludwig Maximilian University of Munich [Germany] (LMU München)-Helmholtz-Zentrum München (HZM), European Molecular Biology Laboratory, Genome Biology Unit, Heidelberg, Germany., Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de génétique humaine (IGH), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Université de Montpellier (UM), and Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM)-Helmholtz-Zentrum München (HZM)-Ludwig Maximilian University of Munich [Germany] (LMU München)
Subjects: 0301 basic medicine, Genetics, Regulation of gene expression, [SDV.GEN]Life Sciences [q-bio]/Genetics, Dosage compensation, [SDV]Life Sciences [q-bio], fungi, Chromosome, Articles, Biology, Biochemistry, Dosage compensation complex, Chromatin, nuclear architecture, Chromosome conformation capture, 03 medical and health sciences, 030104 developmental biology, chromatin, chromosome conformation capture, Molecular Biology, Gene, X chromosome
Abstract: International audience; X chromosome dosage compensation in Drosophila requires chromosome-wide coordination of gene activation. The male-specific lethal dosage compensation complex (DCC) identifies and binds to X-chromosomal high-affinity sites (HAS) from which it boosts transcription. A sub-class of HAS, PionX sites, represent first contacts on the X. Here, we explored the chromosomal interactions of representative PionX sites by high-resolution 4C and determined the global chromosome conformation by Hi-C in sex-sorted embryos. Male and female X chromosomes display similar nuclear architecture, concordant with clustered, constitutively active genes. PionX sites, like HAS, are evenly distributed in the active compartment and engage in short- and long-range interactions beyond compartment boundaries. Long-range, inter-domain interactions between DCC binding sites are stronger in males, suggesting that the complex refines chromatin organization. By de novo induction of DCC in female cells, we monitored the extent of activation surrounding PionX sites. This revealed a remarkable range of DCC action not only in linear proximity, but also at megabase distance if close in space, suggesting that DCC profits from pre-existing chromosome folding to activate genes.
Published: 2017

17. Recombinant tandem of pore-domains in a Weakly Inward rectifying K+ channel 2 (TWIK2) forms active lysosomal channels

Author: Bobak, Nicole, Feliciangeli, Sylvain, Chen, Cheng-Chang, Ben Soussia, Ismail, Bittner, Stefan, Pagnotta, Sophie, Ruck, Tobias, Biel, Martin, Wahl-Schott, Christian, Grimm, Christian, Meuth, Sven G., Lesage, Florian, Institut de pharmacologie moléculaire et cellulaire (IPMC), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA), Center for Integrated Protein Science (CIPSM), Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM)-Helmholtz-Zentrum München (HZM)-Ludwig Maximilian University of Munich [Germany] (LMU München), University Medical Center of the Johannes Gutenberg University Mainz, Centre Commun de Microscopie Appliquée (CCMA), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), University of Münster, and Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM)-Ludwig Maximilian University of Munich [Germany] (LMU München)-Helmholtz-Zentrum München (HZM)
Subjects: Science, [SDV]Life Sciences [q-bio], Gene Expression, Article, Recombinant Proteins, Madin Darby Canine Kidney Cells, Rats, Mice, Protein Transport, Dogs, HEK293 Cells, Potassium Channels, Tandem Pore Domain, Animals, Humans, Medicine, Protein Interaction Domains and Motifs, Amino Acid Sequence, Protein Multimerization, Lysosomes
Abstract: International audience; Recombinant TWIK2 channels produce weak basal background K+ currents. Current amplitudes depend on the animal species the channels have been isolated from and on the heterologous system used for their re-expression. Here we show that this variability is due to a unique cellular trafficking. We identified three different sequence signals responsible for the preferential expression of TWIK2 in the Lamp1-positive lysosomal compartment. Sequential inactivation of tyrosine-based (Y308ASIP) and di-leucine-like (E266LILL and D282EDDQVDIL) trafficking motifs progressively abolishes the targeting of TWIK2 to lysosomes, and promotes its functional relocation at the plasma membrane. In addition, TWIK2 contains two N-glycosylation sites (N79AS and N85AS) on its luminal side, and glycosylation is necessary for expression in lysosomes. As shown by electrophysiology and electron microscopy, TWIK2 produces functional background K+ currents in the endolysosomes, and its expression affects the number and mean size of the lysosomes. These results show that TWIK2 is expressed in lysosomes, further expanding the registry of ion channels expressed in these organelles.
Published: 2017

18. The cryo-EM structure of a ribosome–Ski2-Ski3-Ski8 helicase complex

Author: Christian Schmidt, Markus Pech, Alain Jacquier, Katharina Braunger, Quentin Defenouillère, Micheline Fromont-Racine, Vivekanandan Shanmuganathan, André Heuer, Roland Beckmann, Abdelkader Namane, Elena Conti, Thomas Becker, Otto Berninghausen, Eva Kowalinski, Center for Integrated Protein Science (CIPSM), Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM)-Ludwig-Maximilians-Universität München (LMU)-Helmholtz Zentrum München = German Research Center for Environmental Health, Max-Planck-Institut für Biochemie = Max Planck Institute of Biochemistry (MPIB), Max-Planck-Gesellschaft, Génétique des Interactions macromoléculaires / Genetics of Macromolecular Interactions, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), ED 515 - Complexité du vivant, Université Pierre et Marie Curie - Paris 6 (UPMC), We thank the Leibnitz-Rechenzentrum Munich (LRZ) for providing computational services and support. This work was supported by the German Research Council (SFB646 to R.B, T.B., and E.C., GRK1721 to R.B. and E.C., FOR1805 to R.B, and SFB1035 to E.C), by the Max Planck Gesellschaft (E.C.), and by the Agence Nationale de la Recherche (2011-BSV6-011-02 and ANR-14-CE-10-0014-01 to Q.D., A.J., and M.F.-R.). R.B. and E.C. acknowledge support by the Center for Integrated Protein Science Munich (CiPS-M), the Graduate School of Quantitative Biosciences Munich (QBM), and the European Research Council (Advanced Grants CRYOTRANSLATION and DEAD2THEEND). We also acknowledge the support of Ph.D. and postdoctoral fellowships from Boehringer Ingelheim Fonds (to C.S. and K.B.), from QBM (to V.S.), from the Ministère de l’Enseignement Supérieur et de la Recherche and from the Fondation ARC pour la Recherche sur le Cancer (to Q.D.), and from EMBO, Marie Curie, and the Daimler und Benz Stiftung (to E.K.)., ANR-11-BSV6-0011,NGD-NSD,Rôle du NSD/NGD: identification des gènes cibles et des facteurs impliqués dans ces mécanismes chez Saccharomyces cerevisiae(2011), ANR-14-CE10-0014,CLEANMD,Mécanismes moléculaires et impact de la voie de dégradation des ARNm nonsense (NMD) sur le transcriptome eucaryote.(2014), Technical University of Munich (TUM)-Ludwig Maximilian University of Munich [Germany] (LMU München)-Helmholtz-Zentrum München (HZM), Max-Planck-Institut für Biochemie (MPIB), Génétique des Interactions macromoléculaires, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), and Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM)-Helmholtz-Zentrum München (HZM)-Ludwig Maximilian University of Munich [Germany] (LMU München)
Subjects: 0301 basic medicine, Saccharomyces cerevisiae Proteins, Protein Conformation, RNA Stability, [SDV]Life Sciences [q-bio], MESH: DNA Helicases, Saccharomyces cerevisiae, Ribosome, 03 medical and health sciences, MESH: Saccharomyces cerevisiae Proteins, MESH: Protein Conformation, RNA, Messenger, Ski complex, MESH: RNA, Messenger, Messenger RNA, Multidisciplinary, biology, Exosome Multienzyme Ribonuclease Complex, MESH: RNA, Fungal, Cryoelectron Microscopy, DNA Helicases, Helicase, Nuclear Proteins, RNA, Fungal, MESH: RNA Stability, Ribosomal RNA, Ribosome Subunits, Large, Eukaryotic, Molecular biology, RNA Helicase A, MESH: Saccharomyces cerevisiae, Cell biology, 030104 developmental biology, MESH: Exosome Multienzyme Ribonuclease Complex, MESH: Ribosome Subunits, Large, Eukaryotic, RNA, Ribosomal, TRAMP complex, biology.protein, MESH: RNA, Ribosomal, MESH: Cryoelectron Microscopy, Eukaryotic Ribosome, MESH: Nuclear Proteins
Abstract: Getting rid of faulty mRNA The cell monitors the health of its mRNAs, destroying those that are faulty or damaged. Destruction by the exosome complex prevents them from being used to synthesize deranged and potentially dangerous proteins. Schmidt et al. determined the structure of the Ski helicase complex, which guides RNAs to the exosome complex destruction machinery in association with a mRNAbound ribosome. The end of the mRNA is threaded from the ribosome into the heart of the helicase, whence the message would be channeled into the maw of the exosome complex. Science , this issue p. 1431
Published: 2016

19. The Corporate Samaritan: Advancing Understanding of the Role of Deontic Motive in Justice Enactment

Author: Julia Zwank, Marjo-Riitta Diehl, Mario Gollwitzer, SRH Fernhochschule - The Mobile University, Department of Management Studies, Ludwig Maximilian University of Munich, Aalto-yliopisto, and Aalto University
Subjects: Person strength, Economics and Econometrics, Deontic motive, Moral maturation, Arts and Humanities (miscellaneous), Deontic justice enactment, Supererogation, Organizational justice enactment, Business and International Management, Law, General Business, Management and Accounting, Situation strength
Abstract: Publisher Copyright: © 2023, The Author(s). Although the literature on organizational justice enactment is becoming richer, our understanding of the role of the deontic justice motive remains limited. In this article, we review and discuss theoretical approaches to and evidence of the deontic justice motive and deontic justice enactment. While the prevalent understanding of deontic justice enactment focuses on compliance, we argue that this conceptualization is insufficient to explain behaviors that go beyond the call of duty. We thus consider two further forms of deontic behavior: humanistic and supererogatory behavior. Drawing on the concepts of situation strength and person strength, we further argue that the reduced variance in behavior across morally challenging situations makes deontic justice enactment visible. We thus observe deontic justice enactment when an actor’s deontic justice motive collides with strong situational cues or constraints that guide the actor to behave differently. We formulate propositions and develop a theoretical model that links the deontic justice motive to moral maturation and deontic justice enactment.
Published: 2023

20. Towards robust PICOSEC Micromegas precise timing detectors

Author: Lisowska, M, Angelis, Y, Aune, S, Bortfeldt, J, Brunbauer, F, Chatzianagnostou, E, Dehmelt, K, Desforge, D, Fanourakis, G, Floethner, K.J, Gallinaro, M, Garcia, F, Garg, P, Giomataris, I, Gnanvo, K, Gustavsson, T, Iguaz, F.J, Janssens, D, Kallitsopoulou, A, Kovacic, M, Legou, P, Liu, J, Lupberger, M, Malace, S, Maniatis, I, Meng, Y, Muller, H, Oliveri, E, Orlandini, G, Papaevangelou, T, Pomorski, M, Ropelewski, L, Sampsonidis, D, Scharenberg, L, Schneider, T, Scorsone, E, Sohl, L, van Stenis, M, Tsipolitis, Y, Tzamarias, S.E, Utrobicic, A, Veenhof, R, Wang, X, White, S, Zhang, Z, Zhou, Y, Institut de Recherches sur les lois Fondamentales de l'Univers (IRFU), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Aristotle University of Thessaloniki, Ludwig-Maximilian University of Munich, CERN [Genève], Stony Brook University [SUNY] (SBU), State University of New York (SUNY), Institute of Nuclear and Particle Physics (INPAC), Shanghai Jiao Tong University [Shanghai], Laboratório de Instrumentačão e Física Experimental de Partículas (LIP), Helsinki Institute of Physics (HIP), Helsingin yliopisto = Helsingfors universitet = University of Helsinki, Thomas Jefferson National Accelerator Facility (Jefferson Lab), Laboratoire Interactions, Dynamiques et Lasers (ex SPAM) (LIDyl), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), University of Zagreb, University of Science and Technology of China [Hefei] (USTC), Universität Bonn = University of Bonn, Laboratoire d'Intégration des Systèmes et des Technologies (LIST (CEA)), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Laboratoire Capteurs Diamant (LCD-LIST), Département Métrologie Instrumentation & Information (DM2I), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Laboratoire d'Intégration des Systèmes et des Technologies (LIST (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, National Technical University of Athens [Athens] (NTUA), Ruđer Bošković Institute, the Fundação para a Ciência e a Tecnologia (FCT), Portugal (CERN/FIS-PAR/0005/2021), the US CMS program under DOE contract No. DEAC02-07CH11359, Weizmann Institute of Science, European Project: 665779,H2020,H2020-MSCA-COFUND-2014,COFUND-FP-CERN-2014(2015), and European Project: 600382,EC:FP7:PEOPLE,FP7-PEOPLE-2012-COFUND,ENHANCED EUROTALENTS(2014)
Subjects: instrumentation, Physics - Instrumentation and Detectors, detector, carbon, FOS: Physical sciences, Instrumentation and Detectors (physics.ins-det), cesium iodine, electrode, [PHYS.NEXP]Physics [physics]/Nuclear Experiment [nucl-ex], Cherenkov radiator, muon, radioactivity, [PHYS.PHYS.PHYS-INS-DET]Physics [physics]/Physics [physics]/Instrumentation and Detectors [physics.ins-det], Detectors and Experimental Techniques, boron, ionizing radiation, time resolution, physics.ins-det, Micromegas, performance, nuclear instrumentation
Abstract: International audience; The PICOSEC Micromegas (MM) detector is a precise timing gaseous detector consisting of a Cherenkov radiator combined with a photocathode and a MM amplifying structure. A 100-channel non-resistive PICOSEC MM prototype with 10x10 cm$^2$ active area equipped with a Cesium Iodide (CsI) photocathode demonstrated a time resolution below $\sigma$ =18 ps. The objective of this work is to improve the PICOSEC MM detector robustness aspects; i.e. integration of resistive MM and carbon-based photocathodes; while maintaining good time resolution. The PICOSEC MM prototypes have been tested in laboratory conditions and successfully characterised with 150 GeV/c muon beams at the CERN SPS H4 beam line. The excellent timing performance below $\sigma$ = 20 ps for an individual pad obtained with the 10x10 cm$^2$ area resistive PICOSEC MM of 20 M$\Omega$/sq showed no significant time resolution degradation as a result of adding a resistive layer. A single-pad prototype equipped with a 12 nm thick Boron Carbide (B$_4$C) photocathode presented a time resolution below $\sigma$ = 35 ps; opening up new possibilities for detectors with robust photocathodes. The results made the concept more suitable for the experiments in need of robust detectors with good time resolution.
Published: 2023

21. A large area 100 channel Picosec Micromegas detector with sub 20 ps time resolution

Author: Utrobicic, Antonija, Angelis, Yannis, Aune, Stephan, Bortfeldt, Jonathan, Brunbauer, Florian, Chatzianagnostou, Evridiki, Dehmelt, Klaus, Desforge, Daniel, Fanourakis, George, Floethner, Karl Jonathan, Gallinaro, Michele, Garcia, Francisco, Garg, Prakhar, Giomataris, Ioannis, Gnanvo, Kondo, Gustavsson, Thomas, Iguaz, Francisco Jose, Janssens, Djunes, Kallitsopoulou, Alexandra, Kovacic, Marinko, Legou, Philippe, Lisowska, Marta, Liu, Jianbei, Lupberger, Michael, Malace, Simona, Maniatis, Ioannis, Meng, Yue, Muller, Hans, Oliveri, Eraldo, Orlandini, Giorgio, Papaevangelou, Thomas, Pomorski, Michal, Ropelewski, Leszek, Sampsonidis, Dimos, Scharenberg, Lucian, Schneider, Thomas, Sohl, Lukas, van Stenis, Miranda, Tsipolitis, Yorgos, Tzamarias, Spyros, Veenhof, Rob, Wang, Xu, White, Sebastian, Zhang, Zhiyong, Zhou, Yi, Ruđer Bošković Institute, Aristotle University of Thessaloniki, Ludwig-Maximilian University of Munich, CERN [Genève], Stony Brook University [SUNY] (SBU), State University of New York (SUNY), Institute of Nuclear and Particle Physics (INPAC), Shanghai Jiao Tong University [Shanghai], Universität Bonn = University of Bonn, Laboratório de Instrumentačão e Física Experimental de Partículas (LIP), Helsinki Institute of Physics (HIP), Helsingin yliopisto = Helsingfors universitet = University of Helsinki, Institut de Recherches sur les lois Fondamentales de l'Univers (IRFU), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Thomas Jefferson National Accelerator Facility (Jefferson Lab), Laboratoire Interactions, Dynamiques et Lasers (ex SPAM) (LIDyl), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), University of Zagreb, University of Science and Technology of China [Hefei] (USTC), Laboratoire Capteurs Diamant (LCD-LIST), Département Métrologie Instrumentation & Information (DM2I), Laboratoire d'Intégration des Systèmes et des Technologies (LIST (CEA)), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Laboratoire d'Intégration des Systèmes et des Technologies (LIST (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, National Technical University of Athens [Athens] (NTUA), EPR & D, CERN Strategic Programme on Technologies for Future Experiments, the RD51 collaboration, in the framework of RD51 common projects, the Cross-Disciplinary Program on Instrumentation and Detection of CEA, the French Alternative Energies andAtomic Energy Commission, the PHENIICS Doctoral School Program of Université Paris-Saclay, France, the Fundamental Research Funds for the Central Universities of China, the Program of National Natural Science Foundation of China (grant number 11935014), the Fundação para a Ciência e a Tecnologia (FCT), Portugal (CERN/FIS-PAR/0005/2021), the US CMS program under DOE contract No. DE-AC02-07CH11359, Weizmann Institute of Science, RD51 at CERN, RD51 collaboration, European Project: 665779,H2020,H2020-MSCA-COFUND-2014,COFUND-FP-CERN-2014(2015), and European Project: 600382,EC:FP7:PEOPLE,FP7-PEOPLE-2012-COFUND,ENHANCED EUROTALENTS(2014)
Subjects: instrumentation, MICROPIC, GEM, Physics - Instrumentation and Detectors, detector, Cherenkov detectors, FOS: Physical sciences, Instrumentation and Detectors (physics.ins-det), [PHYS.NEXP]Physics [physics]/Nuclear Experiment [nucl-ex], InGrid, Timing detectors, electronic architecture, RETHGEM, Micropattern gaseous detectors (MSGC, radioactivity, MICROMEGAS, MHSP, [PHYS.PHYS.PHYS-INS-DET]Physics [physics]/Physics [physics]/Instrumentation and Detectors [physics.ins-det], Detectors and Experimental Techniques, ionizing radiation, THGEM, physics.ins-det, nuclear instrumentation
Abstract: The PICOSEC Micromegas precise timing detector is based on a Cherenkov radiator coupled to a photocathode operating in a semi-transparent mode and a Micromegas amplification structure. The first proof of concept single-channel prototype was able to achieve a time resolution below 25 ps. One of the crucial aspects in the development of precise timing gaseous detectors applicable in high-energy physics experiments is a modular design that enables large area coverage. The first 19-channel multi-pad prototype with an active area of approximately 10 cm$^{2}$ suffered from degraded timing resolution due to the non-uniformity of the preamplification gap thickness. A new 100 cm$^{2}$ detector module with 100 channels based on a rigid hybrid ceramic/FR4 Micromegas board for improved drift gap uniformity was developed. Initial measurements with 80 GeV/c muons showed improvements in timing response over the measured pads and a time resolution below 25 ps. More recent measurements with a thinner drift gap detector module and newly developed RF pulse amplifiers show that the pad centre resolution can be enhanced to the level of 17 ps. This work will present the development of the detector from structural simulations, design, and beam test commissioning with a focus on the timing performance of a thinner drift gap detector module in combination with new electronics using an automated timing scan method. The PICOSEC Micromegas precise timing detector is based on a Cherenkov radiator coupled to a semi-transparent photocathode and a Micromegas amplification structure. The first proof of concept single-channel small area prototype was able to achieve time resolution below 25 ps. One of the crucial aspects in the development of the precise timing gaseous detectors applicable in high-energy physics experiments is a modular design that enables large area coverage. The first 19-channel multi-pad prototype with an active area of approximately 10 cm$^2$ suffered from degraded timing resolution due to the non-uniformity of the preamplification gap. A new 100 cm$^2$ detector module with 100 channels based on a rigid hybrid ceramic/FR4 Micromegas board for improved drift gap uniformity was developed. Initial measurements with 80 GeV/c muons showed improvements in timing response over measured pads and a time resolution below 25 ps. More recent measurements with a new thinner drift gap detector module and newly developed RF pulse amplifiers show that the resolution can be enhanced to a level of 17~ps. This work will present the development of the detector from structural simulations, design, and beam test commissioning with a focus on the timing performance of a thinner drift gap detector module in combination with new electronics using an automated timing scan method.
Published: 2023

22. Feeling the Temperature of the Room: Unobtrusive Thermal Display of Engagement during Group Communication

Author: Luke Haliburton, Svenja Yvonne Schött, Linda Hirsch, Robin Welsch, Albrecht Schmidt, Ludwig Maximilian University of Munich, Professorship Welsch Robin, Department of Computer Science, Aalto-yliopisto, and Aalto University
Subjects: Human-Computer Interaction, FOS: Computer and information sciences, Engagement, Thermal Feedback, Computer Networks and Communications, Hardware and Architecture, Affective Computing, Tangible Interfaces, Computer Science - Human-Computer Interaction, Temperature, Human-Computer Interaction (cs.HC)
Abstract: Thermal signals have been explored in HCI for emotion-elicitation and enhancing two-person communication, showing that temperature invokes social and emotional signals in individuals. Yet, extending these findings to group communication is missing. We investigated how thermal signals can be used to communicate group affective states in a hybrid meeting scenario to help people feel connected over a distance. We conducted a lab study (N=20 participants) and explored wrist-worn thermal feedback to communicate audience emotions. Our results show that thermal feedback is an effective method of conveying audience engagement without increasing workload and can help a presenter feel more in tune with the audience. We outline design implications for real-world wearable social thermal feedback systems for both virtual and in-person communication that support group affect communication and social connectedness. Thermal feedback has the potential to connect people across distances and facilitate more effective and dynamic communication in multiple contexts., Comment: In IMWUT 2023
Published: 2023
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23. Pan-cancer Analysis of Homologous Recombination Repair–associated Gene Alterations and Genome-wide Loss-of-Heterozygosity Score

Author: Etienne Rouleau, Luis Garcia Palacios, Clare Turnbull, Joaquin Mateo, Jorge Antonio Lopez, Alexander D Fine, Ethan Sokol, Fabrice Andre, Benedikt Westphalen, Volker Heinemann, Shridar Ganesan, Institut Català de la Salut, [Westphalen CB, Heinemann V] Comprehensive Cancer Center Munich and Department of Medicine III, Ludwig Maximilian University of Munich, Munich, Germany. [Fine AD] Cancer Genomics Research, Foundation Medicine, Inc., Cambridge, Massachusetts. [André F, Rouleau E] Institut Gustave Roussy, INSERM U981, Universite Paris Saclay, Villejuif, France. [Ganesan S] Department of Medicine, Rutgers Cancer Institute of New Jersey, Rutgers University, New Brunswick, New Jersey. [Mateo J] Vall d’Hebron Institute of Oncology (VHIO), Barcelona, Spain. Vall d’Hebron Hospital Universitari, Barcelona, Spain, and Vall d'Hebron Barcelona Hospital Campus
Subjects: Male, Heterozygote, ADN - Reparació, Cancer Research, endocrine system diseases, DNA Repair, PALB2, GLOH, Breast Neoplasms, Poly(ADP-ribose) Polymerase Inhibitors, Biology, Article, neoplasias [ENFERMEDADES], Loss of heterozygosity, Other subheadings::Other subheadings::/genetics [Other subheadings], Humans, Genetic Predisposition to Disease, Recombinació ADN, Homologous Recombination, Càncer, CHEK2, ATRX, BAP1, Otros calificadores::Otros calificadores::/genética [Otros calificadores], Prostatic Neoplasms, Recombinational DNA Repair, FANCA, Neoplasms [DISEASES], Oncology, Cancer research, RAD51C
Abstract: Purpose: To study associations across tumor types between genome-wide loss of heterozygosity (gLOH) and alterations in homologous recombination repair (HRR)-associated genes beyond BRCA1 and BRCA2. Experimental Design: Genomic profiling using a targeted next-generation sequencing assay examining 324–465 genes (FoundationOne, FoundationOne Heme, and FoundationOne CDx; Foundation Medicine, Inc.) was performed in a cohort of 160,790 samples across different tumor types. Zygosity predictions and gLOH status were calculated and linked with alterations in 18 HRR-associated genes (BRCA1, BRCA2, PALB2, BARD1, ATR, ATRX, ATM, BAP1, RAD51B, RAD51C, RAD51D, BRIP1, NBN, CHEK1, CHEK2, FANCA, FANCC, MRE11) and other genomic features, using Fisher's exact test and Mann–Whitney U tests. Results: We identified a strong correlation between elevated gLOH and biallelic alterations in a core set of HRR-associated genes beyond BRCA1 and BRCA2, such as BARD1, PALB2, FANCC, RAD51C, and RAD51D (particularly in breast, ovarian, pancreatic, and prostate cancer). Monoallelic/heterozygous alterations in HRR-associated genes were not associated with elevated gLOH. gLOH was also independently associated with TP53 loss. Co-occurrence of TP53 loss and alterations in HRR-associated genes, and combined loss of TP53-PTEN or TP53-RB1, was associated with a higher gLOH than each of the events separately. Conclusions: Biallelic alterations in core HRR-associated genes are frequent, strongly associated with elevated gLOH, and enriched in breast, ovarian, pancreatic, and prostate cancer. This analysis could inform the design of the next generation of clinical trials examining DNA repair–targeting agents, including PARP inhibitors.
Published: 2021

24. Genetic reactivation of cone photoreceptors restores visual responses in retinitis pigmentosa

Author: Erik Cabuy, Jens Duebel, Michel Paques, José-Alain Sahel, Valérie Forster, Martin Biel, Botond Roska, Peter Humphries, Didier Trono, Mathias Fradot, D. Balya, Serge Picaud, Karl Deisseroth, Sandra Siegert, Mathias W. Seeliger, Saddek Mohand-Said, Volker Busskamp, Anna C. Groner, Tim J. Viney, 'Frontiers in Genetics' program, Ecole Polytechnique Fédérale de Lausanne (EPFL)-National Center of Competence in Research, Neural Circuit Laboratories, Friedrich Miescher Institute for Biomedical Research, Institut de la Vision, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), Diagnostics Research Group, Eberhard-Karls University, Center for Integrated Protein Science (CIPSM), Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM)-Ludwig-Maximilians-Universität München (LMU)-Helmholtz Zentrum München = German Research Center for Environmental Health, Smurfit Institute of Genetics, Trinity College Dublin, Fondation Ophtalmologique Adolphe de Rothschild [Paris], Department of Bioengineering, Department of Psychiatry and Behavioral Sciences, Stanford University, Friedrich Miescher Institute, US Office of Naval Research Naval International Cooperative Opportunities in Science and Technology Program grant, Foundation Fighting Blindness (USA), Swiss National Science Foundation, National Centers of Competence in Research Frontiers in Genetics, ANR-07-TECS-0014,MEDINAS,Matrices d'Electrodes en Diamant pour l'Interfaçage Neuronal Appliqué à la Suppléance fonctionnelle (MEDINAS)(2007), European Project, Fondation Ophtalmologique Adolphe de Rothschild, Marazova, Katia, Technologies pour la santé - Matrices d'Electrodes en Diamant pour l'Interfaçage Neuronal Appliqué à la Suppléance fonctionnelle (MEDINAS) - - MEDINAS2007 - ANR-07-TECS-0014 - TECSAN - VALID, Marie Curie Excellence Grant, EU HEALTH-F2- 223156, RETICIRC - INCOMING, Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM)-Ludwig Maximilian University of Munich [Germany] (LMU München)-Helmholtz-Zentrum München (HZM), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC), and Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM)-Helmholtz-Zentrum München (HZM)-Ludwig Maximilian University of Munich [Germany] (LMU München)
Subjects: Retinal degeneration, Retinal Ganglion Cells, MESH: Evoked Potentials, Visual, genetic structures, Light, Serotypes, Expression, [SDV.GEN] Life Sciences [q-bio]/Genetics, MESH: Dependovirus, MESH: Mice, Knockout, Tissue Culture Techniques, chemistry.chemical_compound, Mice, 0302 clinical medicine, MESH: Genetic Vectors, MESH: Animals, Selectivity, Promoter Regions, Genetic, Halorhodopsin, Mice, Knockout, 0303 health sciences, Multidisciplinary, Halobacteriaceae, Visually guided, MESH: Retina, Retinal Degeneration, Anatomy, Dependovirus, 3. Good health, medicine.anatomical_structure, Retinal Cone Photoreceptor Cells, Halorhodopsins, Retinitis Pigmentosa, Visual phototransduction, MESH: Halorhodopsins, Movement, Genetic Vectors, Biology, Transfection, Retina, 03 medical and health sciences, MESH: Halobacteriaceae, Retinitis pigmentosa, MESH: Promoter Regions, Genetic, medicine, Animals, Humans, Visual Pathways, MESH: Tissue Culture Techniques, MESH: Vision, Ocular, MESH: Mice, Vision, Ocular, 030304 developmental biology, MESH: Visual Pathways, [SDV.GEN]Life Sciences [q-bio]/Genetics, MESH: Humans, Blindness, MESH: Transfection, Ganglion-Cells, Retinal, MESH: Retinal Ganglion Cells, Genetic Therapy, medicine.disease, MESH: Light, eye diseases, Disease Models, Animal, MESH: Retinal Cone Photoreceptor Cells, chemistry, Direction, MESH: Retinitis Pigmentosa, Evoked Potentials, Visual, sense organs, MESH: Disease Models, Animal, MESH: Gene Therapy, Neuroscience, 030217 neurology & neurosurgery, Model
Abstract: Let There Be Light Retinitis pigmentosa, a disease that can result from a wide variety of genetic defects, causes degeneration of photoreceptor cells in the retina and leads to blindness. In the course of the disease, it is generally the rod photoreceptor cells that degenerate first. Cone photoreceptor cells may persist, but in a damaged and nonfunctional state. Busskamp et al. (p. 413 , published online 24 June; see the cover; see the Perspective by Cepko ) have now applied a gene therapy approach to mouse models of retinitis pigmentosa. Inducing expression of a bacterial light-activated ion pump, halorho dopsin, in the damaged cone cells improved visual responses in the diseased mouse retinas. Thus, it may be possible to rescue cone photoreceptors therapeutically, even after they have already been damaged.
Published: 2010

25. Unraveling the interaction between doxorubicin and DNA origami nanostructures for customizable chemotherapeutic drug release

Author: Ijäs, Heini, Shen, Boxuan, Heuer-Jungemann, Amelie, Keller, Adrian, Kostiainen, Mauri A., Liedl, Tim, Ihalainen, Janne A., Linko, Veikko, Biohybrid Materials, Ludwig Maximilian University of Munich, Paderborn University, University of Jyväskylä, Department of Bioproducts and Biosystems, Department of Applied Physics, Aalto-yliopisto, and Aalto University
Subjects: Drug Carriers, Antibiotics, Antineoplastic, AcademicSubjects/SCI00010, organic chemicals, technology, industry, and agriculture, Magnesium Chloride, lääkeaineet, macromolecular substances, DNA, Buffers, nanolääketiede, Nanostructures, carbohydrates (lipids), Drug Liberation, nanorakenteet, Chemical Biology and Nucleic Acid Chemistry, Doxorubicin, polycyclic compounds, Deoxyribonuclease I
Abstract: We thank Dr H. Häkkänen for technical assistance and S. Julin for the 24HB DNA origami design. We acknowledge the provision of facilities and technical support by Aalto University Bioeconomy Facilities and OtaNano – Nanomicroscopy Center (Aalto-NMC). The research was carried out under the Academy of Finland Centres of Excellence Programme (2014–2019). Academy of Finland [308578 to M.A.K.]; Deutsche Forschungsgemeinschaft [Emmy Noether Programme to A.H.-J., SFB1032 (Project A06) to T.L.]; Emil Aaltonen Foundation [to H.I. and V.L.]; Jane and Aatos Erkko Foundation [to J.A.I. and V.L.]; Sigrid Jusélius Foundation [to V.L.]; Vilho, Yrjö and Kalle Väisälä Foundation of the Finnish Academy of Science and Letters [to V.L.]. Funding for open access charge: Emil Aaltonen Foundation.Publisher Copyright: © The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research. Copyright: This record is sourced from MEDLINE/PubMed, a database of the U.S. National Library of Medicine Doxorubicin (DOX) is a common drug in cancer chemotherapy, and its high DNA-binding affinity can be harnessed in preparing DOX-loaded DNA nanostructures for targeted delivery and therapeutics. Although DOX has been widely studied, the existing literature of DOX-loaded DNA-carriers remains limited and incoherent. Here, based on an in-depth spectroscopic analysis, we characterize and optimize the DOX loading into different 2D and 3D scaffolded DNA origami nanostructures (DONs). In our experimental conditions, all DONs show similar DOX binding capacities (one DOX molecule per two to three base pairs), and the binding equilibrium is reached within seconds, remarkably faster than previously acknowledged. To characterize drug release profiles, DON degradation and DOX release from the complexes upon DNase I digestion was studied. For the employed DONs, the relative doses (DOX molecules released per unit time) may vary by two orders of magnitude depending on the DON superstructure. In addition, we identify DOX aggregation mechanisms and spectral changes linked to pH, magnesium, and DOX concentration. These features have been largely ignored in experimenting with DNA nanostructures, but are probably the major sources of the incoherence of the experimental results so far. Therefore, we believe this work can act as a guide to tailoring the release profiles and developing better drug delivery systems based on DNA-carriers.
Published: 2021

26. Intrinsic Differential Scanning Fluorimetry for Fast and Easy Identification of Adeno-Associated Virus Serotypes

Author: Martin Biel, Stylianos Michalakis, Gerhard Winter, Tim Menzen, Axel Rossi, Ruth Rieser, Eduard Ayuso, Hildegard Büning, Magalie Penaud-Budloo, Mohammed Bouzelha, Department of Pharmacy [Munich, Germany] (Center for Drug Research), Ludwig-Maximilians-Universität München (LMU), Laboratoire de Thérapie Génique Translationnelle des Maladies Génétiques (Inserm UMR 1089), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Institute of Experimental Hematology [Hannover, Germany], Hannover Medical School [Hannover] (MHH), Coriolis Pharma [Martinsried, Germany], Center for Integrated Protein Science (CIPSM), Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM)-Helmholtz-Zentrum München (HZM)-Ludwig Maximilian University of Munich [Germany] (LMU München), German Center for Infection Research - partner site Hannover-Braunschweig (DZIF), Department of Ophthalmology [Munich, Germany], This work was supported by the Deutsche Forschungsgemeinschaft (EXC114)., JAULIN, Nicolas, and Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM)-Ludwig-Maximilians-Universität München (LMU)-Helmholtz Zentrum München = German Research Center for Environmental Health
Subjects: Protein Denaturation, Time Factors, [SDV]Life Sciences [q-bio], viruses, Genetic enhancement, Pharmaceutical Science, adeno-associated virus, medicine.disease_cause, melting temperature, 030226 pharmacology & pharmacy, Virus, Workflow, law.invention, high-throughput technology, 03 medical and health sciences, Transduction (genetics), capsid thermal stability, 0302 clinical medicine, law, Gene expression, medicine, Transition Temperature, High throughput technology, Fluorometry, Adeno-associated virus, Fluorescent Dyes, Protein Unfolding, 030304 developmental biology, 0303 health sciences, Protein Stability, Chemistry, intrinsic fluorescence, Dependovirus, High-Throughput Screening Assays, Cell biology, [SDV] Life Sciences [q-bio], AAV vector, Capsid, Recombinant DNA, Capsid Proteins, Hydrophobic and Hydrophilic Interactions
Abstract: International audience; Recombinant adeno-associated virus (AAV) vectors have evolved as the most promising technology for gene therapy due to their good safety profile, high transduction efficacy, and long-term gene expression in non-dividing cells. AAV-based gene therapy holds great promise for treating genetic disorders like inherited blindness, muscular atrophy, or bleeding disorders. Multiple naturally occurring and engineered AAV serotypes exist, which differ in capsid sequence and as a consequence in cellular tropism. Individual AAV capsids differ in thermal stability and have a characteristic melting temperature (Tm), which enables serotype-specific discrimination of AAV vectors. Differential scanning fluorimetry (DSF) combined with a dye-like SYPRO Orange (SO-DSF), which binds to hydrophobic regions of unfolded proteins, has been successfully applied to determine the Tm of AAV capsids. Here, we present DSF measurement of intrinsic fluorescence signal (iDSF) as a simple alternative method for determination of AAV capsid Tm. The study demonstrates that DSF measurement of intrinsic fluorescence signal is a simple, accurate, and rapid alternative to SO-DSF, which enables characterization of AAV capsid stability with excellent precision and without the need of SO or any other dye.
Published: 2020

27. CMB-S4

Author: Collaboration, The CMB-S4, Abazajian, Kevork, Addison, Graeme E., Adshead, Peter, Ahmed, Zeeshan, Akerib, Daniel, Ali, Aamir, Allen, Steven W., Alonso, David, Alvarez, Marcelo, Amin, Mustafa A., Anderson, Adam, Arnold, Kam S., Ashton, Peter, Baccigalupi, Carlo, Bard, Debbie, Barkats, Denis, Barron, Darcy, Barry, Peter S., Bartlett, James G., Thakur, Ritoban Basu, Battaglia, Nicholas, Bean, Rachel, Bebek, Chris, Bender, Amy N., Benson, Bradford A., Bianchini, Federico, Bischoff, Colin A., Bleem, Lindsey, Bock, James J., Bocquet, Sebastian, Boddy, Kimberly K., Bond, J. Richard, Borrill, Julian, Bouchet, François R., Brinckmann, Thejs, Brown, Michael L., Bryan, Sean, Buza, Victor, Byrum, Karen, Caimapo, Carlos Hervias, Calabrese, Erminia, Calafut, Victoria, Caldwell, Robert, Carlstrom, John E., Carron, Julien, Cecil, Thomas, Challinor, Anthony, Chang, Clarence L., Chinone, Yuji, Cho, Hsiao-Mei Sherry, Cooray, Asantha, Coulton, Will, Crawford, Thomas M., Crites, Abigail, Cukierman, Ari, Cyr-Racine, Francis-Yan, de Haan, Tijmen, Delabrouille, Jacques, Devlin, Mark, Di Valentino, Eleonora, Dierickx, Marion, Dobbs, Matt, Duff, Shannon, Dunkley, Jo, Dvorkin, Cora, Eimer, Joseph, Elleflot, Tucker, Errard, Josquin, Essinger-Hileman, Thomas, Fabbian, Giulio, Feng, Chang, Ferraro, Simone, Filippini, Jeffrey P., Flauger, Raphael, Flaugher, Brenna, Fraisse, Aurelien A., Frolov, Andrei, Galitzki, Nicholas, Gallardo, Patricio A., Galli, Silvia, Ganga, Ken, Gerbino, Martina, Gluscevic, Vera, Goeckner-Wald, Neil, Green, Daniel, Grin, Daniel, Grohs, Evan, Gualtieri, Riccardo, Gudmundsson, Jon E., Gullett, Ian, Gupta, Nikhel, Habib, Salman, Halpern, Mark, Halverson, Nils W., Hanany, Shaul, Harrington, Kathleen, Hasegawa, Masaya, Hasselfield, Matthew, Hazumi, Masashi, Heitmann, Katrin, Henderson, Shawn, Hensley, Brandon, Hill, Charles, Hill, J. Colin, Hlozek, Renée, Ho, Shuay-Pwu Patty, Hoang, Thuong, Holder, Gil, Holzapfel, William, Hood, John, Hubmayr, Johannes, Huffenberger, Kevin M., Hui, Howard, Irwin, Kent, Jeong, Oliver, Johnson, Bradley R., Jones, William C., Kang, Jae Hwan, Karkare, Kirit S., Katayama, Nobuhiko, Keskitalo, Reijo, Kisner, Theodore, Knox, Lloyd, Koopman, Brian J., Kosowsky, Arthur, Kovac, John, Kovetz, Ely D., Kuhlmann, Steve, Kuo, Chao-lin, Kusaka, Akito, Lähteenmäki, Anne, Lawrence, Charles R., Lee, Adrian T., Lewis, Antony, Li, Dale, Linder, Eric, Loverde, Marilena, Lowitz, Amy, Lubin, Phil, Madhavacheril, Mathew S., Mantz, Adam, Marques, Gabriela, Matsuda, Frederick, Mauskopf, Philip, McCarrick, Heather, McMahon, Jeffrey, Meerburg, P. Daniel, Melin, Jean-Baptiste, Menanteau, Felipe, Meyers, Joel, Millea, Marius, Mohr, Joseph, Moncelsi, Lorenzo, Monzani, Maria, Mroczkowski, Tony, Mukherjee, Suvodip, Nagy, Johanna, Namikawa, Toshiya, Nati, Federico, Natoli, Tyler, Newburgh, Laura, Niemack, Michael D., Nishino, Haruki, Nord, Brian, Novosad, Valentine, O'Brient, Roger, Padin, Stephen, Palladino, Steven, Partridge, Bruce, Petravick, Don, Pierpaoli, Elena, Pogosian, Levon, Prabhu, Karthik, Pryke, Clement, Puglisi, Giuseppe, Racine, Benjamin, Rahlin, Alexandra, Rao, Mayuri Sathyanarayana, Raveri, Marco, Reichardt, Christian L., Remazeilles, Mathieu, Rocha, Graca, Roe, Natalie A., Roy, Anirban, Ruhl, John E., Salatino, Maria, Saliwanchik, Benjamin, Schaan, Emmanuel, Schillaci, Alessandro, Schmitt, Benjamin, Schmittfull, Marcel M., Scott, Douglas, Sehgal, Neelima, Shandera, Sarah, Sherwin, Blake D., Shirokoff, Erik, Simon, Sara M., Slosar, Anze, Spergel, David, Germaine, Tyler St., Staggs, Suzanne T., Stark, Antony, Starkman, Glenn D., Stompor, Radek, Stoughton, Chris, Suzuki, Aritoki, Tajima, Osamu, Teply, Grant P., Thompson, Keith, Thorne, Ben, Timbie, Peter, Tomasi, Maurizio, Tristram, Matthieu, Tucker, Gregory, Umiltà, Caterina, van Engelen, Alexander, Vavagiakis, Eve M., Vieira, Joaquin D., Vieregg, Abigail G., Wagoner, Kasey, Wallisch, Benjamin, Wang, Gensheng, Watson, Scott, Westbrook, Ben, Whitehorn, Nathan, Wollack, Edward J., Wu, W. L. Kimmy, Xu, Zhilei, Yang, H. Y. Eric, Yasini, Siavash, Yefremenko, Volodymyr G., Yoon, Ki Won, Young, Edward, Yu, Cyndia, Zonca, Andrea, AstroParticule et Cosmologie (APC (UMR_7164)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Observatoire de Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Institut d'Astrophysique de Paris (IAP), Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Institut de Physique Théorique - UMR CNRS 3681 (IPHT), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Centre de Physique des Particules de Marseille (CPPM), Aix Marseille Université (AMU)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physique des 2 Infinis Irène Joliot-Curie (IJCLab), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), CMB-S4, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Université Paris-Sud - Paris 11 (UP11), Apollo - University of Cambridge Repository, Van Swinderen Institute for Particle Physics and G, Cosmic Frontier, Abazajian, K, Addison, G, Adshead, P, Ahmed, Z, Akerib, D, Ali, A, Allen, S, Alonso, D, Alvarez, M, Amin, M, Anderson, A, Arnold, K, Ashton, P, Baccigalupi, C, Bard, D, Barkats, D, Barron, D, Barry, P, Bartlett, J, Basu Thakur, R, Battaglia, N, Bean, R, Bebek, C, Bender, A, Benson, B, Bianchini, F, Bischoff, C, Bleem, L, Bock, J, Bocquet, S, Boddy, K, Richard Bond, J, Borrill, J, Bouchet, F, Brinckmann, T, Brown, M, Bryan, S, Buza, V, Byrum, K, Hervias Caimapo, C, Calabrese, E, Calafut, V, Caldwell, R, Carlstrom, J, Carron, J, Cecil, T, Challinor, A, Chang, C, Chinone, Y, Sherry Cho, H, Cooray, A, Coulton, W, Crawford, T, Crites, A, Cukierman, A, Cyr-Racine, F, De Haan, T, Delabrouille, J, Devlin, M, Di Valentino, E, Dierickx, M, Dobbs, M, Duff, S, Dvorkin, C, Eimer, J, Elleflot, T, Errard, J, Essinger-Hileman, T, Fabbian, G, Feng, C, Ferraro, S, Filippini, J, Flauger, R, Flaugher, B, Fraisse, A, Frolov, A, Galitzki, N, Gallardo, P, Galli, S, Ganga, K, Gerbino, M, Gluscevic, V, Goeckner-Wald, N, Green, D, Grin, D, Grohs, E, Gualtieri, R, Gudmundsson, J, Gullett, I, Gupta, N, Habib, S, Halpern, M, Halverson, N, Hanany, S, Harrington, K, Hasegawa, M, Hasselfield, M, Hazumi, M, Heitmann, K, Henderson, S, Hensley, B, Hill, C, Colin Hill, J, Hlozek, R, Patty Ho, S, Hoang, T, Holder, G, Holzapfel, W, Hood, J, Hubmayr, J, Huffenberger, K, Hui, H, Irwin, K, Jeong, O, Johnson, B, Jones, W, Hwan Kang, J, Karkare, K, Katayama, N, Keskitalo, R, Kisner, T, Knox, L, Koopman, B, Kosowsky, A, Kovac, J, Kovetz, E, Kuhlmann, S, Kuo, C, Kusaka, A, Lahteenmaki, A, Lawrence, C, Lee, A, Lewis, A, Li, D, Linder, E, Loverde, M, Lowitz, A, Lubin, P, Madhavacheril, M, Mantz, A, Marques, G, Matsuda, F, Mauskopf, P, Mccarrick, H, Mcmahon, J, Daniel Meerburg, P, Melin, J, Menanteau, F, Meyers, J, Millea, M, Mohr, J, Moncelsi, L, Monzani, M, Mroczkowski, T, Mukherjee, S, Nagy, J, Namikawa, T, Nati, F, Natoli, T, Newburgh, L, Niemack, M, Nishino, H, Nord, B, Novosad, V, O'Brient, R, Padin, S, Palladino, S, Partridge, B, Petravick, D, Pierpaoli, E, Pogosian, L, Prabhu, K, Pryke, C, Puglisi, G, Racine, B, Rahlin, A, Sathyanarayana Rao, M, Raveri, M, Reichardt, C, Remazeilles, M, Rocha, G, Roe, N, Roy, A, Ruhl, J, Salatino, M, Saliwanchik, B, Schaan, E, Schillaci, A, Schmitt, B, Schmittfull, M, Scott, D, Sehgal, N, Shandera, S, Sherwin, B, Shirokoff, E, Simon, S, Slosar, A, Spergel, D, S, T, Staggs, S, Stark, A, Starkman, G, Stompor, R, Stoughton, C, Suzuki, A, Tajima, O, Teply, G, Thompson, K, Thorne, B, Timbie, P, Tomasi, M, Tristram, M, Tucker, G, Umilta, C, Van Engelen, A, Vavagiakis, E, Vieira, J, Vieregg, A, Wagoner, K, Wallisch, B, Wang, G, Watson, S, Westbrook, B, Whitehorn, N, Wollack, E, Kimmy Wu, W, Xu, Z, Eric Yang, H, Yasini, S, Yefremenko, V, Won Yoon, K, Young, E, Yu, C, Zonca, A, University of California Irvine, Johns Hopkins University, University of Illinois at Urbana-Champaign, SLAC National Accelerator Laboratory, University of California Berkeley, Stanford University, University of Oxford, Rice University, Fermi National Accelerator Laboratory, University of California San Diego, International School for Advanced Studies, Lawrence Berkeley National Laboratory, Harvard University, University of New Mexico, Argonne National Laboratory, Université Paris-Diderot, California Institute of Technology, Cornell University, University of Melbourne, University of Cincinnati, Ludwig Maximilian University of Munich, University of Toronto, UMR7095, Stony Brook University, University of Manchester, Arizona State University, Florida State University, Cardiff University, Dartmouth College, University of Geneva, University of Cambridge, The University of Tokyo, University of Groningen, University of Chicago, University of Pennsylvania, McGill University, National Institute of Standards and Technology, NASA Goddard Space Flight Center, University of Sussex, Princeton University, Simon Fraser University, National Institute for Nuclear Physics, Haverford College, Stockholm University, Case Western Reserve University, University of British Columbia, University of Colorado Boulder, University of Minnesota Twin Cities, University of Michigan, Ann Arbor, Simons Foundation, Columbia University, University of Virginia, University of California Davis, Yale University, University of Pittsburgh, Ben-Gurion University of the Negev, Department of Electronics and Nanoengineering, Jet Propulsion Laboratory, University of California Santa Barbara, Perimeter Institute for Theoretical Physics, Service d'Astrophysique CEA, European Southern Observatory, Washington University St. Louis, University of Milan - Bicocca, University of Southern California, Institute for Advanced Studies, Pennsylvania State University, Brookhaven National Laboratory, Kyoto University, University of Wisconsin-Madison, University of Milano, Université Paris-Saclay, Brown University, Syracuse University, University of California Los Angeles, Aalto-yliopisto, and Aalto University
Subjects: Astrophysics and Astronomy, Cosmology and Nongalactic Astrophysics (astro-ph.CO), Cosmology, CMB, Settore FIS/05, Space and Planetary Science, astro-ph.CO, FOS: Physical sciences, Astronomy and Astrophysics, Astrophysics::Cosmology and Extragalactic Astrophysics, [PHYS.ASTR]Physics [physics]/Astrophysics [astro-ph], Galaxies and Cosmology, Astrophysics - Cosmology and Nongalactic Astrophysics
Abstract: CMB-S4---the next-generation ground-based cosmic microwave background (CMB) experiment---is set to significantly advance the sensitivity of CMB measurements and enhance our understanding of the origin and evolution of the Universe, from the highest energies at the dawn of time through the growth of structure to the present day. Among the science cases pursued with CMB-S4, the quest for detecting primordial gravitational waves is a central driver of the experimental design. This work details the development of a forecasting framework that includes a power-spectrum-based semi-analytic projection tool, targeted explicitly towards optimizing constraints on the tensor-to-scalar ratio, $r$, in the presence of Galactic foregrounds and gravitational lensing of the CMB. This framework is unique in its direct use of information from the achieved performance of current Stage 2--3 CMB experiments to robustly forecast the science reach of upcoming CMB-polarization endeavors. The methodology allows for rapid iteration over experimental configurations and offers a flexible way to optimize the design of future experiments given a desired scientific goal. To form a closed-loop process, we couple this semi-analytic tool with map-based validation studies, which allow for the injection of additional complexity and verification of our forecasts with several independent analysis methods. We document multiple rounds of forecasts for CMB-S4 using this process and the resulting establishment of the current reference design of the primordial gravitational-wave component of the Stage-4 experiment, optimized to achieve our science goals of detecting primordial gravitational waves for $r > 0.003$ at greater than $5��$, or, in the absence of a detection, of reaching an upper limit of $r < 0.001$ at $95\%$ CL., 24 pages, 8 figures, 9 tables, submitted to ApJ. arXiv admin note: text overlap with arXiv:1907.04473
Published: 2022

28. National identity predicts public health support during a global pandemic

Author: Van Bavel, Jay J., Cichocka, Aleksandra, Capraro, Valerio, Sjåstad, Hallgeir, Nezlek, John B., Pavlović, Tomislav, Alfano, Mark, Gelfand, Michele J., Azevedo, Flavio, Birtel, Michèle D., Cislak, Aleksandra, Lockwood, Patricia L., Ross, Robert Malcolm, Abts, Koen, Agadullina, Elena, Aruta, John Jamir Benzon, Besharati, Sahba Nomvula, Bor, Alexander, Choma, Becky L., Crabtree, Charles David, Cunningham, William A., De, Koustav, Ejaz, Waqas, Elbaek, Christian T., Findor, Andrej, Flichtentrei, Daniel, Franc, Renata, Gjoneska, Biljana, Gruber, June, Gualda, Estrella, Horiuchi, Yusaku, Huynh, Toan Luu Duc, Ibanez, Agustin, Imran, Mostak Ahamed, Israelashvili, Jacob, Jasko, Katarzyna, Kantorowicz, Jaroslaw, Kantorowicz-Reznichenko, Elena, Krouwel, André, Laakasuo, Michael, Lamm, Claus, Leygue, Caroline, Lin, Ming-Jen, Mansoor, Mohammad Sabbir, Marie, Antoine, Mayiwar, Lewend, Mazepus, Honorata, McHugh, Cillian, Minda, John Paul, Mitkidis, Panagiotis, Olsson, Andreas, Otterbring, Tobias, Packer, Dominic J., Perry, Anat, Petersen, Michael Bang, Puthillam, Arathy, Riaño-Moreno, Julián C., Rothmund, Tobias, Santamaría-García, Hernando, Schmid, Petra C., Stoyanov, Drozdstoy, Tewari, Shruti, Todosijević, Bojan, Tsakiris, Manos, Tung, Hans H., Umbreș, Radu G., Vanags, Edmunds, Vlasceanu, Madalina, Vonasch, Andrew, Yucel, Meltem, Zhang, Yucheng, Abad, Mohcine, Adler, Eli, Akrawi, Narin, Mdarhri, Hamza Alaoui, Amara, Hanane, Amodio, David M., Antazo, Benedict G., Apps, Matthew, Ay, F. Ceren, Ba, Mouhamadou Hady, Barbosa, Sergio, Bastian, Brock, Berg, Anton, Bernal-Zárate, Maria P., Bernstein, Michael, Białek, Michał, Bilancini, Ennio, Bogatyreva, Natalia, Boncinelli, Leonardo, Booth, Jonathan E., Borau, Sylvie, Buchel, Ondrej, Cameron, C. Daryl, Carvalho, Chrissie F., Celadin, Tatiana, Cerami, Chiara, Chalise, Hom Nath, Cheng, Xiaojun, Cian, Luca, Cockcroft, Kate, Conway, Jane, Córdoba-Delgado, Mateo Andres, Crespi, Chiara, Crouzevialle, Marie, Cutler, Jo, Cypryańska, Marzena, Dabrowska, Justyna, Daniels, Michael A., Davis, Victoria H., Dayley, Pamala N., Delouvee, Sylvain, Denkovski, Ognjan, Dezecache, Guillaume, Dhaliwal, Nathan A., Diato, Alelie B., Di Paolo, Roberto, Drosinou, Marianna, Dulleck, Uwe, Ekmanis, Jānis, Ertan, Arhan S., Etienne, Tom W., Farhana, Hapsa Hossain, Farkhari, Fahima, Farmer, Harry, Fenwick, Ali, Fidanovski, Kristijan, Flew, Terry, Fraser, Shona, Frempong, Raymond Boadi, Fugelsang, Jonathan A., Gale, Jessica, Garcia-Navarro, E. Begoña, Garladinne, Prasad, Ghajjou, Oussama, Gkinopoulos, Theofilos, Gray, Kurt, Griffin, Siobhán M., Gronfeldt, Bjarki, Gümren, Mert, Gurung, Ranju Lama, Halperin, Eran, Harris, Elizabeth, Herzon, Volo, Hruška, Matej, Huang, Guanxiong, Hudecek, Matthias F. C., Isler, Ozan, Jangard, Simon, Jørgensen, Frederik J., Kachanoff, Frank, Kahn, John, Dangol, Apsara Katuwal, Keudel, Oleksandra, Koppel, Lina, Koverola, Mika, Kubin, Emily, Kunnari, Anton, Kutiyski, Yordan, Laguna, Oscar, Leota, Josh, Lermer, Eva, Levy, Jonathan, Levy, Neil, Li, Chunyun, Long, Elizabeth U., Longoni, Chiara, Maglić, Marina, McCashin, Darragh, Metcalf, Alexander L., Mikloušić, Igor, El Mimouni, Soulaimane, Miura, Asako, Molina-Paredes, Juliana, Monroy-Fonseca, César, Morales-Marente, Elena, Moreau, David, Muda, Rafał, Myer, Annalisa, Nash, Kyle, Nesh-Nash, Tarik, Nitschke, Jonas P., Nurse, Matthew S., Ohtsubo, Yohsuke, Oldemburgo De Mello, Victoria, O’Madagain, Cathal, Onderco, Michal, Palacios-Galvez, M. Soledad, Palomäki, Jussi, Pan, Yafeng, Papp, Zsófia, Pärnamets, Philip, Paruzel-Czachura, Mariola, Pavlović, Zoran, Payán-Gómez, César, Perander, Silva, Pitman, Michael Mark, Prasad, Rajib, Pyrkosz-Pacyna, Joanna, Rathje, Steve, Raza, Ali, Rêgo, Gabriel G., Rhee, Kasey, Robertson, Claire E., Rodríguez-Pascual, Iván, Saikkonen, Teemu, Salvador-Ginez, Octavio, Sampaio, Waldir M., Santi, Gaia C., Santiago-Tovar, Natalia, Savage, David, Scheffer, Julian A., Schönegger, Philipp, Schultner, David T., Schutte, Enid M., Scott, Andy, Sharma, Madhavi, Sharma, Pujan, Skali, Ahmed, Stadelmann, David, Stafford, Clara Alexandra, Stanojević, Dragan, Stefaniak, Anna, Sternisko, Anni, Stoica, Agustin, Stoyanova, Kristina K., Strickland, Brent, Sundvall, Jukka, Thomas, Jeffrey P., Tinghög, Gustav, Torgler, Benno, Traast, Iris J., Tucciarelli, Raffaele, Tyrala, Michael, Ungson, Nick D., Uysal, Mete S., Van Lange, Paul A. M., Van Prooijen, Jan-Willem, Van Rooy, Dirk, Västfjäll, Daniel, Verkoeijen, Peter, Vieira, Joana B., Von Sikorski, Christian, Walker, Alexander Cameron, Watermeyer, Jennifer, Wetter, Erik, Whillans, Ashley, Willardt, Robin, Wohl, Michael J. A., Wójcik, Adrian Dominik, Wu, Kaidi, Yamada, Yuki, Yilmaz, Onurcan, Yogeeswaran, Kumar, Ziemer, Carolin-Theresa, Zwaan, Rolf A., Boggio, Paulo S., Van Bavel, Jay J., Cichocka, Aleksandra, Capraro, Valerio, Sjåstad, Hallgeir, Nezlek, John B., Pavlović, Tomislav, Alfano, Mark, Gelfand, Michele J., Azevedo, Flavio, Birtel, Michèle D., Cislak, Aleksandra, Lockwood, Patricia L., Ross, Robert Malcolm, Abts, Koen, Agadullina, Elena, Aruta, John Jamir Benzon, Besharati, Sahba Nomvula, Bor, Alexander, Choma, Becky L., Crabtree, Charles David, Cunningham, William A., De, Koustav, Ejaz, Waqa, Elbaek, Christian T., Findor, Andrej, Flichtentrei, Daniel, Franc, Renata, Gjoneska, Biljana, Gruber, June, Gualda, Estrella, Horiuchi, Yusaku, Huynh, Toan Luu Duc, Ibanez, Augustin, Imran, Mostak Ahamed, Israelashvili, Jacob, Jasko, Katarzyna, Kantorowicz, Jaroslaw, Kantorowicz-Reznichenko, Elena, Krouwel, André, Laakasuo, Michael, Lamm, Clau, Leygue, Caroline, Lin, Ming-Jen, Mansoor, Mohammad Sabbir, Marie, Antoine, Mayiwar, Lewend, Mazepus, Honorata, McHugh, Cillian, Minda, John Paul, Mitkidis, Panagioti, Olsson, Andrea, Otterbring, Tobia, Packer, Dominic J., Perry, Anat, Petersen, Michael Bang, Puthillam, Arathy, Riaño-Moreno, Julián C., Rothmund, Tobia, Santamaría-García, Hernando, Schmid, Petra C., Stoyanov, Drozdstoy, Tewari, Shruti, Todosijević, Bojan, Tsakiris, Mano, Tung, Hans H., Umbreș, Radu G., Vanags, Edmund, Vlasceanu, Madalina, Vonasch, Andrew, Yucel, Meltem, Zhang, Yucheng, Abad, Mohcine, Adler, Eli, Akrawi, Narin, Mdarhri, Hamza Alaoui, Amara, Hanane, Amodio, David M., Antazo, Benedict G., Apps, Matthew, Ay, F. Ceren, Ba, Mouhamadou Hady, Barbosa, Sergio, Bastian, Brock, Berg, Anton, Bernal-Zárate, Maria P., Bernstein, Michael, Białek, Michał, Bilancini, Ennio, Bogatyreva, Natalia, Boncinelli, Leonardo, Booth, Jonathan E., Borau, Sylvie, Buchel, Ondrej, Cameron, C. Daryl, Carvalho, Chrissie F., Celadin, Tatiana, Cerami, Chiara, Chalise, Hom Nath, Cheng, Xiaojun, Cian, Luca, Cockcroft, Kate, Conway, Jane, Córdoba-Delgado, Mateo Andre, Crespi, Chiara, Crouzevialle, Marie, Cutler, Jo, Cypryańska, Marzena, Dabrowska, Justyna, Daniels, Michael A., Davis, Victoria H., Dayley, Pamala N., Delouvee, Sylvain, Denkovski, Ognjan, Dezecache, Guillaume, Dhaliwal, Nathan A., Diato, Alelie B., Di Paolo, Roberto, Drosinou, Marianna, Dulleck, Uwe, Ekmanis, Jāni, Ertan, Arhan S., Etienne, Tom W., Farhana, Hapsa Hossain, Farkhari, Fahima, Farmer, Harry, Fenwick, Ali, Fidanovski, Kristijan, Flew, Terry, Fraser, Shona, Frempong, Raymond Boadi, Fugelsang, Jonathan A., Gale, Jessica, Garcia-Navarro, E. Begoña, Garladinne, Prasad, Ghajjou, Oussama, Gkinopoulos, Theofilo, Gray, Kurt, Griffin, Siobhán M., Gronfeldt, Bjarki, Gümren, Mert, Gurung, Ranju Lama, Halperin, Eran, Harris, Elizabeth, Herzon, Volo, Hruška, Matej, Huang, Guanxiong, Hudecek, Matthias F. C., Isler, Ozan, Jangard, Simon, Jørgensen, Frederik J., Kachanoff, Frank, Kahn, John, Dangol, Apsara Katuwal, Keudel, Oleksandra, Koppel, Lina, Koverola, Mika, Kubin, Emily, Kunnari, Anton, Kutiyski, Yordan, Laguna, Oscar, Leota, Josh, Lermer, Eva, Levy, Jonathan, Levy, Neil, Li, Chunyun, Long, Elizabeth U., Longoni, Chiara, Maglić, Marina, McCashin, Darragh, Metcalf, Alexander L., Mikloušić, Igor, El Mimouni, Soulaimane, Miura, Asako, Molina-Paredes, Juliana, Monroy-Fonseca, César, Morales-Marente, Elena, Moreau, David, Muda, Rafał, Myer, Annalisa, Nash, Kyle, Nesh-Nash, Tarik, Nitschke, Jonas P., Nurse, Matthew S., Ohtsubo, Yohsuke, Oldemburgo de Mello, Victoria, O’Madagain, Cathal, Onderco, Michal, Palacios-Galvez, M. Soledad, Palomäki, Jussi, Pan, Yafeng, Papp, Zsófia, Pärnamets, Philip, Paruzel-Czachura, Mariola, Pavlović, Zoran, Payán-Gómez, César, Perander, Silva, Pitman, Michael Mark, Prasad, Rajib, Pyrkosz-Pacyna, Joanna, Rathje, Steve, Raza, Ali, Rêgo, Gabriel G., Rhee, Kasey, Robertson, Claire E., Rodríguez-Pascual, Iván, Saikkonen, Teemu, Salvador-Ginez, Octavio, Sampaio, Waldir M., Santi, Gaia C., Santiago-Tovar, Natalia, Savage, David, Scheffer, Julian A., Schönegger, Philipp, Schultner, David T., Schutte, Enid M., Scott, Andy, Sharma, Madhavi, Sharma, Pujan, Skali, Ahmed, Stadelmann, David, Stafford, Clara Alexandra, Stanojević, Dragan, Stefaniak, Anna, Sternisko, Anni, Stoica, Augustin, Stoyanova, Kristina K., Strickland, Brent, Sundvall, Jukka, Thomas, Jeffrey P., Tinghög, Gustav, Torgler, Benno, Traast, Iris J., Tucciarelli, Raffaele, Tyrala, Michael, Ungson, Nick D., Uysal, Mete S., Van Lange, Paul A. M., van Prooijen, Jan-Willem, van Rooy, Dirk, Västfjäll, Daniel, Verkoeijen, Peter, Vieira, Joana B., von Sikorski, Christian, Walker, Alexander Cameron, Watermeyer, Jennifer, Wetter, Erik, Whillans, Ashley, Willardt, Robin, Wohl, Michael J. A., Wójcik, Adrian Dominik, Wu, Kaidi, Yamada, Yuki, Yilmaz, Onurcan, Yogeeswaran, Kumar, Ziemer, Carolin-Theresa, Zwaan, Rolf A., Boggio, Paulo S., Department of Digital Humanities, Faculty Common Matters (Faculty of Arts), Cognitive Science, Helsinki Research Hub on Religion, Media and Social Change, Helsinki Social Computing Group, Doctoral Programme in Cognition, Learning, Instruction and Communication, Mind and Matter, High Performance Cognition group, Medicum, Digital Humanities, Law and Economics, Pharmacy, Public Administration, Molecular Genetics, Research Methods and Techniques, Brain and Cognition, Van Bavel, Jay J [0000-0002-2520-0442], Cichocka, Aleksandra [0000-0003-1703-1586], Sjåstad, Hallgeir [0000-0002-8730-1038], Nezlek, John B [0000-0003-4963-3637], Pavlović, Tomislav [0000-0002-4470-3715], Alfano, Mark [0000-0001-5879-8033], Azevedo, Flavio [0000-0001-9000-8513], Cislak, Aleksandra [0000-0002-9880-6947], Lockwood, Patricia L [0000-0001-7195-9559], Ross, Robert Malcolm [0000-0001-8711-1675], Abts, Koen [0000-0001-8546-8347], Agadullina, Elena [0000-0002-1505-1412], Aruta, John Jamir Benzon [0000-0003-4155-1063], Besharati, Sahba Nomvula [0000-0003-2836-7982], Bor, Alexander [0000-0002-2624-9221], Crabtree, Charles David [0000-0001-5144-8671], De, Koustav [0000-0001-9562-0672], Ejaz, Waqas [0000-0002-2492-4115], Elbaek, Christian T [0000-0002-7039-4565], Findor, Andrej [0000-0002-5896-6989], Franc, Renata [0000-0002-1909-2393], Gjoneska, Biljana [0000-0003-1200-6672], Huynh, Toan Luu Duc [0000-0002-1486-127X], Ibanez, Augustin [0000-0001-6758-5101], Imran, Mostak Ahamed [0000-0002-5101-3149], Kantorowicz, Jaroslaw [0000-0002-1186-5427], Krouwel, André [0000-0003-0952-6028], Laakasuo, Michael [0000-0003-2826-6073], Lamm, Claus [0000-0002-5422-0653], Leygue, Caroline [0000-0002-0355-1030], Lin, Ming-Jen [0000-0002-7174-2226], Mansoor, Mohammad Sabbir [0000-0002-6541-3506], Marie, Antoine [0000-0002-7958-0153], McHugh, Cillian [0000-0002-9701-3232], Minda, John Paul [0000-0002-4081-010X], Mitkidis, Panagiotis [0000-0002-9495-7369], Olsson, Andreas [0000-0001-5272-7744], Otterbring, Tobias [0000-0002-0283-8777], Perry, Anat [0000-0003-2329-856X], Petersen, Michael Bang [0000-0002-6782-5635], Riaño-Moreno, Julián C [0000-0003-4182-0550], Rothmund, Tobias [0000-0003-2979-5129], Schmid, Petra C [0000-0002-9990-5445], Stoyanov, Drozdstoy [0000-0002-9975-3680], Todosijević, Bojan [0000-0002-6116-993X], Tsakiris, Manos [0000-0001-7753-7576], Tung, Hans H [0000-0001-5332-7582], Vanags, Edmunds [0000-0003-1932-936X], Vlasceanu, Madalina [0000-0003-2138-1968], Yucel, Meltem [0000-0002-7274-5971], Zhang, Yucheng [0000-0001-9435-6734], Abad, Mohcine [0000-0002-4964-5411], Mdarhri, Hamza Alaoui [0000-0001-9831-6561], Amara, Hanane [0000-0003-0732-2320], Antazo, Benedict G [0000-0001-9993-8960], Apps, Matthew [0000-0001-5793-2202], Barbosa, Sergio [0000-0003-1989-158X], Bastian, Brock [0000-0003-4619-3322], Bernal-Zárate, Maria P [0000-0001-8232-6220], Białek, Michał [0000-0002-5062-5733], Boncinelli, Leonardo [0000-0003-0626-5133], Booth, Jonathan E [0000-0002-8563-4613], Borau, Sylvie [0000-0003-1564-0695], Buchel, Ondrej [0000-0002-0139-5513], Chalise, Hom Nath [0000-0002-9301-6890], Cian, Luca [0000-0002-8051-1366], Cockcroft, Kate [0000-0002-6166-8050], Conway, Jane [0000-0003-3883-349X], Córdoba-Delgado, Mateo Andres [0000-0002-2264-7388], Crouzevialle, Marie [0000-0002-5538-6030], Cutler, Jo [0000-0003-1073-764X], Dabrowska, Justyna [0000-0002-8821-7161], Davis, Victoria H [0000-0002-7207-4629], Dayley, Pamala N [0000-0001-8955-9502], Delouvee, Sylvain [0000-0002-4029-597X], Di Paolo, Roberto [0000-0002-6081-6656], Dulleck, Uwe [0000-0002-0953-5963], Ekmanis, Jānis [0000-0003-1781-1785], Etienne, Tom W [0000-0002-4299-6593], Farkhari, Fahima [0000-0002-8484-5128], Farmer, Harry [0000-0002-3684-0605], Fenwick, Ali [0000-0002-5412-9745], Flew, Terry [0000-0003-4485-9338], Frempong, Raymond Boadi 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[0000-0003-1787-5914], Palomäki, Jussi [0000-0001-6063-0926], Pan, Yafeng [0000-0002-5633-8313], Pavlović, Zoran [0000-0002-9231-5100], Payán-Gómez, César [0000-0002-0633-1332], Perander, Silva [0000-0001-6711-8079], Pitman, Michael Mark [0000-0001-5532-5388], Pyrkosz-Pacyna, Joanna [0000-0002-9112-8629], Raza, Ali [0000-0002-2438-6054], Rhee, Kasey [0000-0002-8562-0801], Rodríguez-Pascual, Iván [0000-0002-5385-3643], Saikkonen, Teemu [0000-0001-9619-3270], Sampaio, Waldir M [0000-0002-6066-4314], Schönegger, Philipp [0000-0001-9930-487X], Schultner, David T [0000-0003-2253-4065], Scott, Andy [0000-0002-3294-0078], Skali, Ahmed [0000-0002-4753-3280], Stadelmann, David [0000-0002-1211-9936], Stafford, Clara Alexandra [0000-0003-1716-7870], Stanojević, Dragan [0000-0002-3667-2461], Stefaniak, Anna [0000-0002-1706-7784], Sternisko, Anni [0000-0002-2507-3076], Stoica, Augustin [0000-0003-0585-1114], Sundvall, Jukka [0000-0003-4310-1162], Tinghög, Gustav [0000-0002-8159-1249], Torgler, Benno [0000-0002-9809-963X], Tucciarelli, Raffaele [0000-0002-0342-308X], Tyrala, Michael [0000-0001-5268-8319], Van Lange, Paul AM [0000-0001-7774-6984], van Prooijen, Jan-Willem [0000-0001-6236-0819], Västfjäll, Daniel [0000-0003-2873-4500], von Sikorski, Christian [0000-0002-3787-8277], Walker, Alexander Cameron [0000-0003-1431-6770], Watermeyer, Jennifer [0000-0001-7918-8832], Whillans, Ashley [0000-0002-1726-6978], Willardt, Robin [0000-0002-2495-3450], Wohl, Michael JA [0000-0001-6945-5562], Wójcik, Adrian Dominik [0000-0002-7073-6019], Wu, Kaidi [0000-0001-6881-7437], Yamada, Yuki [0000-0003-1431-568X], Yilmaz, Onurcan [0000-0002-6094-7162], Ziemer, Carolin-Theresa [0000-0002-0794-7702], Apollo - University of Cambridge Repository, Communication Science, Network Institute, Communication Choices, Content and Consequences (CCCC), Social Psychology, IBBA, A-LAB, New York University [New York] (NYU), NYU System (NYU), University of Kent [Canterbury], Middlesex University [London], Norwegian School of Economics and Business Administration, SWPS University of Social Sciences and Humanities (SWPS), College of William and Mary [Williamsburg] (WM), Ivo Pilar Institute of Social Sciences, Macquarie University, Stanford University, Friedrich-Schiller-Universität = Friedrich Schiller University Jena [Jena, Germany], University of Greenwich, University of Oxford, University of Birmingham [Birmingham], Catholic University of Leuven - Katholieke Universiteit Leuven (KU Leuven), Vysšaja škola èkonomiki = National Research University Higher School of Economics [Moscow] (HSE), De La Salle University [Manila] (DLSU), University of the Witwatersrand [Johannesburg] (WITS), Aarhus University [Aarhus], University of Toronto, Dartmouth College [Hanover], University of Kentucky (UK), National University of Sciences and Technology [Islamabad] (NUST), Comenius University in Bratislava, IntraMed, Buenos Aires, DF, Argentina, Macedonian Academy of Sciences and Arts [Skopje, North Macedonia] (MASA), University of Colorado [Boulder], Universidad de Huelva, WHU-Otto Beisheim School of Management, University Adolfo Ibanez (Santiago), University of Dhaka, The Hebrew University of Jerusalem (HUJ), Uniwersytet Jagielloński w Krakowie = Jagiellonian University (UJ), Universiteit Leiden, Erasmus University Rotterdam, Vrije Universiteit Amsterdam [Amsterdam] (VU), Helsingin yliopisto = Helsingfors universitet = University of Helsinki, University of Vienna [Vienna], Universidad Nacional Autónoma de México = National Autonomous University of Mexico (UNAM), National Taiwan University [Taiwan] (NTU), Tribhuvan University, BI Norwegian Business School [Oslo], University of Limerick (UL), University of Western Ontario (UWO), Duke University [Durham], Karolinska Institute, University of Agder (UIA), Lehigh University [Bethlehem], Monk Prayogshala, Cooperative University of Colombia, Pontificia Universidad Javeriana (PUJ), Department of Management, Technology, and Economics [ETH Zürich] (D-MTEC), Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich), Medical University of Plovdiv, Indian Institute of Management Indore (IMM Indore), Institute of Social Sciences Belgrade, University of London [London], University of Luxembourg [Luxembourg], National School of Political and Administrative Studies (SNSPA), University of Latvia (LU), Princeton University, University of Canterbury [Christchurch], University of Virginia, Hebei University of Technology [Tianjin], Université Mohammed VI Polytechnique [Ben Guerir] (UM6P), Institute for Research and Development-Kurdistan, University of Amsterdam [Amsterdam] (UvA), Jose Rizal Memorial State University, Université Cheikh Anta Diop [Dakar, Sénégal] (UCAD), Universidad del Rosario [Bogota], University of Melbourne, Penn State Abington, Pennsylvania Commonwealth System of Higher Education (PCSHE)-Penn State System, University of Wrocław [Poland] (UWr), IMT Alti Studi Lucca, Università degli Studi di Firenze = University of Florence (UniFI), London School of Economics and Political Science (LSE), Toulouse School of Economics (TSE-R), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-École des hautes études en sciences sociales (EHESS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Tilburg University [Tilburg], Netspar, Pennsylvania State University (Penn State), Penn State System, Universidade Federal de Santa Catarina = Federal University of Santa Catarina [Florianópolis] (UFSC), University of Bologna/Università di Bologna, Istituto Universitario di Studi Superiori (IUSS), Shenzhen University [Shenzhen], Institute for Advanced Study in Toulouse (IAST), Università degli Studi di Pavia = University of Pavia (UNIPV), Cracow University of Economics, University of British Columbia (UBC), University of California [Los Angeles] (UCLA), University of California (UC), Laboratoire de Psychologie : Cognition, Comportement, Communication (LP3C - EA1285), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Université de Rennes 2 (UR2)-Institut Brestois des Sciences de l'Homme et de la Société (IBSHS), Université de Brest (UBO), Laboratoire de Psychologie Sociale et Cognitive (LAPSCO), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA), University of British Columbia [Vancouver], Cavite State University-General Trias City Campus, School for Advanced Studies Lucca (IMT), Queensland University of Technology [Brisbane] (QUT), Australian National University (ANU), Boǧaziçi üniversitesi = Boğaziçi University [Istanbul], Kieskompas, Hult International Business School Dubai, The University of Sydney, University of Bayreuth, University of Waterloo [Waterloo], University of Bradford, University of Crete [Heraklion] (UOC), University of North Carolina [Chapel Hill] (UNC), University of North Carolina System (UNC), Koç University, City University of Hong Kong [Hong Kong] (CUHK), University of Regensburg, Universität Duisburg-Essen = University of Duisburg-Essen [Essen], Free University of Berlin (FU), Linköping University (LIU), University of Koblenz-Landau, University of Alberta, Ludwig Maximilian University [Munich] (LMU), Interdisciplinary Center Herzliya (IDC), Aalto University, Macquarie University [Sydney], Boston University [Boston] (BU), Dublin City University [Dublin] (DCU), University of Montana, Osaka University [Osaka], SEELE Neuroscience, University of Auckland [Auckland], Maria Curie-Sklodowska University (UMCS), CUNY Graduate Center (The Graduate Center), City University of New York [New York] (CUNY), The University of Tokyo (UTokyo), Mohammed VI Polytechnic University, Hungarian Academy of Sciences (MTA), Medical University of Silesia (SUM), University of Belgrade [Belgrade], Vidyasagar College For Women, AGH University of Science and Technology [Krakow, PL] (AGH UST), University of Cambridge [UK] (CAM), Mackenzie Presbyterian University [São Paulo] (UPM), University of Turku, University of Newcastle [Callaghan, Australia] (UoN), University of St Andrews [Scotland], University of Groningen [Groningen], Carleton University, National University of Political Studies and Public Administration Bucharest, Romania (SNSPA), University of Plovdiv, Institut Jean-Nicod (IJN), Département d'Etudes Cognitives - ENS Paris (DEC), École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École des hautes études en sciences sociales (EHESS)-Collège de France (CdF (institution))-Centre National de la Recherche Scientifique (CNRS)-Département de Philosophie - ENS Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), Center for Research in Economics, Management and the Arts (CREMA), Partenaires INRAE, University College of London [London] (UCL), The Hong Kong University of Science and Technology, Susquehanna University, Dokuz Eylül Üniversitesi = Dokuz Eylül University [Izmir] (DEÜ), Stockholm School of Economics (SSE), Harvard Business School, Harvard University, Nicolaus Copernicus University [Toruń], University of California [San Diego] (UC San Diego), Kyushu University, Kadir Has University (KHAS), Van Bavel, Jay J. [0000-0002-2520-0442], Nezlek, John B. [0000-0003-4963-3637], Lockwood, Patricia L. [0000-0001-7195-9559], Elbaek, Christian T. [0000-0002-7039-4565], Ibanez, Agustin [0000-0001-6758-5101], Riaño-Moreno, Julián C. [0000-0003-4182-0550], Schmid, Petra C. [0000-0002-9990-5445], Tung, Hans H. [0000-0001-5332-7582], Antazo, Benedict G. [0000-0001-9993-8960], Bernal-Zárate, Maria P. [0000-0001-8232-6220], Booth, Jonathan E. [0000-0002-8563-4613], Davis, Victoria H. [0000-0002-7207-4629], Dayley, Pamala N. [0000-0001-8955-9502], Etienne, Tom W. [0000-0002-4299-6593], Garcia-Navarro, E. Begoña [0000-0001-6913-8882], Griffin, Siobhán M. [0000-0002-3613-2844], Hudecek, Matthias F. C. [0000-0002-7696-766X], Jørgensen, Frederik J. [0000-0002-5461-912X], Metcalf, Alexander L. [0000-0001-9532-585X], Nitschke, Jonas P. [0000-0002-3244-8585], Nurse, Matthew S. [0000-0003-1787-5914], Sampaio, Waldir M. [0000-0002-6066-4314], Schultner, David T. [0000-0003-2253-4065], Stoica, Agustin [0000-0003-0585-1114], Van Lange, Paul A. M. [0000-0001-7774-6984], Wohl, Michael J. A. [0000-0001-6945-5562], New York University, University of Kent, Middlesex University, Norwegian School of Economics, College of William and Mary, Institute of Social Sciences Ivo Pilar, Friedrich Schiller University Jena, SWPS University of Social Sciences and Humanities, KU Leuven, Higher School of Economics, De La Salle University Manila, University of the Witwatersrand, Aarhus University, X University, Dartmouth College, University of Kentucky, National University of Sciences and Technology Pakistan, IntraMed, Macedonian Academy of Sciences and Arts, University of Colorado Boulder, University of Huelva, Otto Beisheim School of Management, Universidad Adolfo Ibáñez, Hebrew University of Jerusalem, Jagiellonian University in Kraków, Leiden University, Vrije Universiteit Amsterdam, University of Helsinki, University of Vienna, Universidad Nacional Autónoma de México, National Taiwan University, BI Norwegian Business School, University of Limerick, Western University, Karolinska Institutet, University of Agder, Lehigh University, Universidad Cooperativa de Colombia, Universidad Javeriana, Swiss Federal Institute of Technology Zurich, Indian Institute of Management Indore, Institute of Social Sciences, Royal Holloway University of London, University of Latvia, University of Canterbury, Duke University, Hebei University of Technology, University of Kurdistan, Impact For Development, Jose Rizal University, University of Birmingham, Université Cheikh Anta Diop de Dakar, Universidad del Rosario, Pennsylvania State University, University of Wrocław, IMT Institute for Advanced Studies Lucca, University of Florence, Birkbeck University of London, Toulouse Business School, Tilburg University, Universidade Federal de Santa Catarina, Universita di Bologna, University of Pavia, Shenzhen University, Université Toulouse 1 Capitole, IRCCS Fondazione Istituto Neurologico Casimiro Mondino - Pavia, University of British Columbia, University of California Los Angeles, University of Rennes 2, University of Amsterdam, Université Clermont Auvergne, Cavite State University, Queensland University of Technology, Bogazici University, University of Sydney, University of Waterloo, University of North Carolina at Chapel Hill, Koc University, City University of Hong Kong, Free University of Berlin, Linköping University, Ludwig Maximilian University of Munich, Department of Neuroscience and Biomedical Engineering, Boston University, Dublin City University, Osaka University, University of Auckland, Maria Curie-Sklodowska University, Australian National University, The University of Tokyo, Hungarian Academy of Sciences, University of Silesia in Katowice, University of Belgrade, University of Calcutta, AGH University of Science and Technology, University of Cambridge, Universidade Presbiteriana Mackenzie, University of Newcastle, University of St Andrews, University of Groningen, National School of Political and Administrative Studies, University College London, Hong Kong University of Science and Technology, Dokuz Eylul University, Stockholm School of Economics, Nicolaus Copernicus University in Toruń, University of California San Diego, Kadir Has University, Aalto-yliopisto, University of St Andrews. School of International Relations, University of St Andrews. School of Psychology and Neuroscience, Van Bavel, J, Cichocka, A, Capraro, V, Sjastad, H, Nezlek, J, Pavlovic, T, Alfano, M, Gelfand, M, Azevedo, F, Birtel, M, Cislak, A, Lockwood, P, Ross, R, Abts, K, Agadullina, E, Aruta, J, Besharati, S, Bor, A, Choma, B, Crabtree, C, Cunningham, W, De, K, Ejaz, W, Elbaek, C, Findor, A, Flichtentrei, D, Franc, R, Gjoneska, B, Gruber, J, Gualda, E, Horiuchi, Y, Huynh, T, Ibanez, A, Imran, M, Israelashvili, J, Jasko, K, Kantorowicz, J, Kantorowicz-Reznichenko, E, Krouwel, A, Laakasuo, M, Lamm, C, Leygue, C, Lin, M, Mansoor, M, Marie, A, Mayiwar, L, Mazepus, H, Mchugh, C, Minda, J, Mitkidis, P, Olsson, A, Otterbring, T, Packer, D, Perry, A, Petersen, M, Puthillam, A, Riano-Moreno, J, Rothmund, T, Santamaria-Garcia, H, Schmid, P, Stoyanov, D, Tewari, S, Todosijevic, B, Tsakiris, M, Tung, H, Umbres, R, Vanags, E, Vlasceanu, M, Vonasch, A, Yucel, M, Zhang, Y, Abad, M, Adler, E, Akrawi, N, Mdarhri, H, Amara, H, Amodio, D, Antazo, B, Apps, M, Ay, F, Ba, M, Barbosa, S, Bastian, B, Berg, A, Bernal-Zarate, M, Bernstein, M, Bialek, M, Bilancini, E, Bogatyreva, N, Boncinelli, L, Booth, J, Borau, S, Buchel, O, Cameron, C, Carvalho, C, Celadin, T, Cerami, C, Chalise, H, Cheng, X, Cian, L, Cockcroft, K, Conway, J, Cordoba-Delgado, M, Crespi, C, Crouzevialle, M, Cutler, J, Cypryanska, M, Dabrowska, J, Daniels, M, Davis, V, Dayley, P, Delouvee, S, Denkovski, O, Dezecache, G, Dhaliwal, N, Diato, A, Di Paolo, R, Drosinou, M, Dulleck, U, Ekmanis, J, Ertan, A, Etienne, T, Farhana, H, Farkhari, F, Farmer, H, Fenwick, A, Fidanovski, K, Flew, T, Fraser, S, Frempong, R, Fugelsang, J, Gale, J, Garcia-Navarro, E, Garladinne, P, Ghajjou, O, Gkinopoulos, T, Gray, K, Griffin, S, Gronfeldt, B, Gumren, M, Gurung, R, Halperin, E, Harris, E, Herzon, V, Hruska, M, Huang, G, Hudecek, M, Isler, O, Jangard, S, Jorgensen, F, Kachanoff, F, Kahn, J, Dangol, A, Keudel, O, Koppel, L, Koverola, M, Kubin, E, Kunnari, A, Kutiyski, Y, Laguna, O, Leota, J, Lermer, E, Levy, J, Levy, N, Li, C, Long, E, Longoni, C, Maglic, M, Mccashin, D, Metcalf, A, Miklousic, I, El Mimouni, S, Miura, A, Molina-Paredes, J, Monroy-Fonseca, C, Morales-Marente, E, Moreau, D, Muda, R, Myer, A, Nash, K, Nesh-Nash, T, Nitschke, J, Nurse, M, Ohtsubo, Y, Oldemburgo de Mello, V, O'Madagain, C, Onderco, M, Palacios-Galvez, M, Palomaki, J, Pan, Y, Papp, Z, Parnamets, P, Paruzel-Czachura, M, Pavlovic, Z, Payan-Gomez, C, Perander, S, Pitman, M, Prasad, R, Pyrkosz-Pacyna, J, Rathje, S, Raza, A, Rego, G, Rhee, K, Robertson, C, Rodriguez-Pascual, I, Saikkonen, T, Salvador-Ginez, O, Sampaio, W, Santi, G, Santiago-Tovar, N, Savage, D, Scheffer, J, Schonegger, P, Schultner, D, Schutte, E, Scott, A, Sharma, M, Sharma, P, Skali, A, Stadelmann, D, Stafford, C, Stanojevic, D, Stefaniak, A, Sternisko, A, Stoica, A, Stoyanova, K, Strickland, B, Sundvall, J, Thomas, J, Tinghog, G, Torgler, B, Traast, I, Tucciarelli, R, Tyrala, M, Ungson, N, Uysal, M, Van Lange, P, van Prooijen, J, van Rooy, D, Vastfjall, D, Verkoeijen, P, Vieira, J, von Sikorski, C, Walker, A, Watermeyer, J, Wetter, E, Whillans, A, Willardt, R, Wohl, M, Wojcik, A, Wu, K, Yamada, Y, Yilmaz, O, Yogeeswaran, K, Ziemer, C, Zwaan, R, and Boggio, P
Subjects: IMAGE, Health Behavior, COVID-19, national identity, public health, pandemic, cross-cultural, Collective narcissism, Settore SECS-P/02 - Politica Economica, health behavior, Sociology, RA0421 Public health. Hygiene. Preventive Medicine, Settore SECS-P/01 - Economia Politica, public health behaviours, COVID-19, collective behaviour, Public health, [SHS.SOCIO]Humanities and Social Sciences/Sociology, Social Identification, 706/689/477/2811, article, Social identity, Public Health, Global Health, Social Medicine and Epidemiology, [SHS.ECO]Humanities and Social Sciences/Economics and Finance, 3142 Public health care science, environmental and occupational health, VDP::Medisinske Fag: 700::Helsefag: 800, 5141 Sociology, Settore SECS-P/03 - Scienza delle Finanze, National identity, Human, Cross-Cultural Comparison, BF Psychology, Science, COVID-19 pandemic, BF, national narcissism, HV Social pathology. Social and public welfare. Criminology, Humans, Leadership, Pandemics, Public Health, SARS-CoV-2, Self Report, Social Conformity, Human development, 692/699/255/2514, SDG 3 - Good Health and Well-being, Human behaviour, political ideology, COLLECTIVE NARCISSISM, SOCIAL IDENTITY, MCC, Pandemic, IDENTIFICATION, DAS, [SHS.SCIPO]Humanities and Social Sciences/Political science, Coronavirus, MODEL, Folkhälsovetenskap, global hälsa, socialmedicin och epidemiologi, Viral infection, Idenfication, Image, RA Public aspects of medicine, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Human medicine, RA, Model
Abstract: Funder: Research Council of Norway through its Centres of Excellence Scheme, FAIR project No 262675, Funder: J. William Fulbright Program, Funder: Institute for Lifecourse Development, University of Greenwich, Funder: Economic Social Research Council Impact Acceleration Award, University of Oxford, Funder: Institute of Social Sciences Ivo Pilar, Funder: Academy of Finland (Suomen Akatemia); doi: https://doi.org/10.13039/501100002341, Funder: Universität Wien (University of Vienna); doi: https://doi.org/10.13039/501100003065, Funder: Ministry of Science and Technology, Taiwan (Ministry of Science and Technology of Taiwan); doi: https://doi.org/10.13039/501100004663, Funder: NOMIS Stiftung (NOMIS Foundation); doi: https://doi.org/10.13039/501100008483, Funder: Princeton Graduate Student Research Funding (Program in Cognitive Science), Funder: Corruption Laboratory on Ethics, Accountability, and the Rule of Law (CLEAR), University of Virginia, Funder: Project Pro.Co.P.E., IMT School (PAI2019), Funder: Italian Ministry of University and Research - PRIN 2017 (20178293XT), Funder: Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada (Conseil de Recherches en Sciences Naturelles et en Génie du Canada); doi: https://doi.org/10.13039/501100000038, Funder: Australian Research Council (DP180102384), Funder: Ernst and Young (EY); doi: https://doi.org/10.13039/501100003064, Changing collective behaviour and supporting non-pharmaceutical interventions is an important component in mitigating virus transmission during a pandemic. In a large international collaboration (Study 1, N = 49,968 across 67 countries), we investigated self-reported factors associated with public health behaviours (e.g., spatial distancing and stricter hygiene) and endorsed public policy interventions (e.g., closing bars and restaurants) during the early stage of the COVID-19 pandemic (April-May 2020). Respondents who reported identifying more strongly with their nation consistently reported greater engagement in public health behaviours and support for public health policies. Results were similar for representative and non-representative national samples. Study 2 (N = 42 countries) conceptually replicated the central finding using aggregate indices of national identity (obtained using the World Values Survey) and a measure of actual behaviour change during the pandemic (obtained from Google mobility reports). Higher levels of national identification prior to the pandemic predicted lower mobility during the early stage of the pandemic (r = −0.40). We discuss the potential implications of links between national identity, leadership, and public health for managing COVID-19 and future pandemics.
Published: 2022

29. Inequality in mortality between Black and White Americans by age, place, and cause and in comparison to Europe, 1990 to 2018

Author: Eddy van Doorslaer, Josselin Thuilliez, Ana Rodríguez-González, Bram Wouterse, Stefano Lombardi, René Karadakic, Paola Bertoli, Libertad Gonzalez, Janet Currie, Joachim Winter, Kjell G. Salvanes, Kristiina Huttunen, Sarah Cattan, Amelie Wuppermann, Peter Redler, Marlies Bär, Cláudia Costa, Veronica Grembi, Hannes Schwandt, Aline Bütikofer, Carlos Riumallo-Herl, Lucy Kraftman, James Banks, Paula Santana, Beatrice Zong-Ying Chao, Tom Van Ourti, Sonya Krutikova, Northwestern University [Evanston], National Bureau of Economic Research [New York] (NBER), The National Bureau of Economic Research (NBER), Center for Health and Wellbeing, Princeton University, Princeton University, Erasmus School of Health Policy and Management |Rotterdam], University of Manchester [Manchester], Institute for Fiscal Studies, Department of Economics and SAFE Center, University of Verona, University of Verona (UNIVR), Norwegian School of Economics and Business Administration, Department of Economics (NHH), Norwegian School of Economics and Business Administration, The Institute for Fiscal Studies (IFS), The Institute for Fiscal Studies, University of Coimbra [Portugal] (UC), Universitat Pompeu Fabra [Barcelona] (UPF), Department of Statistics and Quantitative Methods University of Milano-Bicocca, Università degli Studi di Milano-Bicocca [Milano] (UNIMIB), Aalto University, Government Institute for Economic Research, Helsinki (VATT), VATT, University of Munich (LMU Munich), Department of Economics, Faculty of Economics, Erasmus University Rotterdam, Erasmus university, Lund University [Lund], Centre d'économie de la Sorbonne (CES), Université Paris 1 Panthéon-Sorbonne (UP1)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS), Erasmus University Rotterdam, Martin-Luther-University Halle-Wittenberg, Claudia Costa received support from the Science and Technology Foundation (FCT), the European Social Fund, and the Centro Operational Programme (SFRH/BD/132218/2017). Paula Santana received support from the Centre of Studies in Geography and Spatial Planning (UIDB/04084/2020), through an FCT fund. Aline Bütikofer, René Karadakic, and Kjell Salvanes received support from the Research Council of Norway through project No. 275800 and through its Centres of Excellence Scheme, FAIR project No. 262675 and by the NORFACE DIAL grant 462-16-050. Peter Redler received support from the Elite Network of Bavaria within the Evidence-Based Economics programme., Northwestern University, University of Verona, Norwegian School of Economics, Universidade de Coimbra, Universitat Pompeu Fabra, University of Milano, Department of Economics, VATT Institute for Economic Research, Ludwig Maximilian University of Munich, Lund University, Université Paris 1 Panthéon-Sorbonne, Martin Luther University Halle-Wittenberg, and Aalto-yliopisto
Subjects: Mortality/ethnology, area-level socioeconomic status, Area-level socioeconomic status, Life expectancy, Social Sciences, 0302 clinical medicine, Homicide, JEL: I - Health, Education, and Welfare/I.I1 - Health, Age-specific mortality, 030212 general & internal medicine, 050207 economics, Young adult, Child, International comparison, media_common, JEL: J - Labor and Demographic Economics/J.J1 - Demographic Economics, Multidisciplinary, Mortality rate, 05 social sciences, 1. No poverty, Middle Aged, 16. Peace & justice, [SHS.ECO]Humanities and Social Sciences/Economics and Finance, 3. Good health, Europe, Geography, Blacks/statistics & numerical data, Child, Preschool, Adult, age-specific mortality, international comparison, life expectancy, racial divide, Coronavirus disease 2019 (COVID-19), Inequality, Adolescent, media_common.quotation_subject, Black People, Life Expectancy/ethnology, Economic Sciences, White People, 03 medical and health sciences, Young Adult, Life Expectancy, 0502 economics and business, Humans, Mortality, Mortality trends, Aged, White (horse), JEL: E - Macroeconomics and Monetary Economics/E.E2 - Consumption, Saving, Production, Investment, Labor Markets, and Informal Economy/E.E2.E21 - Consumption • Saving • Wealth, Whites/statistics & numerical data, Infant, United States, Racial divide, Demography
Abstract: Significance From 1990 to 2018, the Black–White American life expectancy gap fell 48.9% and mortality inequality decreased, although progress stalled after 2012 as life expectancy plateaued. Had improvements continued at the 1990 to 2012 rate, the racial gap in life expectancy would have closed by 2036. Despite decreasing mortality inequality, income-based life expectancy gaps remain starker in the United States than in European countries. At the same time, European mortality improved strongly and even those U.S. populations with the longest life spans–White Americans living in the highest-income areas–experience higher mortality at all ages than Europeans in high-income areas in 2018. Hence, mortality rates of both Black and White Americans could fall much further in both high-income and low-income areas., Although there is a large gap between Black and White American life expectancies, the gap fell 48.9% between 1990 and 2018, mainly due to mortality declines among Black Americans. We examine age-specific mortality trends and racial gaps in life expectancy in high- and low-income US areas and with reference to six European countries. Inequalities in life expectancy are starker in the United States than in Europe. In 1990, White Americans and Europeans in high-income areas had similar overall life expectancy, while life expectancy for White Americans in low-income areas was lower. However, since then, even high-income White Americans have lost ground relative to Europeans. Meanwhile, the gap in life expectancy between Black Americans and Europeans decreased by 8.3%. Black American life expectancy increased more than White American life expectancy in all US areas, but improvements in lower-income areas had the greatest impact on the racial life expectancy gap. The causes that contributed the most to Black Americans’ mortality reductions included cancer, homicide, HIV, and causes originating in the fetal or infant period. Life expectancy for both Black and White Americans plateaued or slightly declined after 2012, but this stalling was most evident among Black Americans even prior to the COVID-19 pandemic. If improvements had continued at the 1990 to 2012 rate, the racial gap in life expectancy would have closed by 2036. European life expectancy also stalled after 2014. Still, the comparison with Europe suggests that mortality rates of both Black and White Americans could fall much further across all ages and in both high-income and low-income areas.
Published: 2021

30. Neurodevelopment vs. the immune system: complementary contributions of maternally-inherited gene transcripts and proteins to successful egg development in fish

Author: Katarzyna Dryl, Jérôme Montfort, Christophe Klopp, Joanna Nynca, Daniel Żarski, Slawomir Ciesielski, Andrzej Ciereszko, Thomas Fröhlich, Miwako Kösters, Pascal Fontaine, Beata Sarosiek, Jarosław Król, Aurélie Le Cam, Julien Bobe, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Ludwig Maximilian University of Munich [Germany] (LMU München), Unité de Mathématiques et Informatique Appliquées de Toulouse (MIAT INRA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), University of Warmia and Mazury [Olsztyn], Stanislaw Sakowicz Inland Fisheries Institute, Unité de Recherches Animal et Fonctionnalités des Produits Animaux (URAFPA), Université de Lorraine (UL)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and This work was supported by the National Science Center for Poland (NCN) [grant number8532016/22/M/NZ9/0059
Subjects: 0303 health sciences, Reproductive success, [SDV]Life Sciences [q-bio], media_common.quotation_subject, 030302 biochemistry & molecular biology, Embryogenesis, egg quality, Embryo, embryo lethality, pikeperch, Biology, Oocyte, Cell biology, reproduction, Transcriptome, transcriptomics, 03 medical and health sciences, proteomics, medicine.anatomical_structure, medicine, Maternal to zygotic transition, Gene, Ovulation, 030304 developmental biology, media_common
Abstract: BackgroundIn Metazoans, embryonic development relies on maternally-inherited mRNAs and proteins that are critical for early developmental success and known to play major roles at later stages, beyond zygotic genome activation. However, very poor concordance between transcript and protein levels in oocytes and embryos of vertebrates suggest that maternally-inherited proteins and maternally-inherited mRNAs are playing different roles in unfertilized eggs, not considered to date comprehensively. The aim of this study was to investigate the respective contribution of maternally-inherited mRNAs and maternally-inherited proteins to egg molecular cargo and to its developmental competence using pikeperch, an ecologically and commercially relevant freshwater fish species, as a model.ResultsOur data shed new light on the importance of maternally-inherited mRNAs in nervous system development suggesting that neurogenesis is a major mRNA-dependent non-genetic inheritance factor. In contrast, our results highlight a specific role of maternally-inherited proteins in immune response in ovulated eggs suggesting that maternal proteins would rather contribute to developmental success through protection of the embryo against pathogens. Further analysis revealed susceptibility of the transcriptome to modifications during the post-vitellogenic processes (i.e., final oocyte maturation and ovulation), whereas proteomic cargo remains unaffected. This may negatively affect developmental competence of the egg and possibly influence further nervous system development of the embryo.ConclusionsOur study provides novel insights into the understanding of type-specific roles of maternally-inherited molecules in fish. Here we show, for the first time, that transcripts and proteins have distinct, yet complementary, functions in the egg of teleost fish. Maternally-inherited mRNAs would shape embryo neurodevelopment and possibly the future behavior of the fish, while maternally-inherited proteins would rather be responsible for protecting the embryo against pathogens. Additionally, we observed that processes directly preceding ovulation may considerably affect the reproductive success by modifying expression level of genes crucial for proper embryonic development, being novel fish egg quality markers (e.g., smarca4 or h3f3a). These results are of major importance for understanding the influence of external factors on reproductive fitness in both captive and wild-type fish species.
Published: 2021

31. DNA Origami Route for Nanophotonics

Author: Guillermo P. Acuna, Na Liu, Ralf Jungmann, Anton Kuzyk, Department of Neuroscience and Biomedical Engineering, Ludwig Maximilian University of Munich, Braunschweig University of Technology, Max Planck Institute for Intelligent Systems, Aalto-yliopisto, and Aalto University
Subjects: Base pair, Computer science, Nanophotonics, FOS: Physical sciences, Nanotechnology, 02 engineering and technology, 010402 general chemistry, Fluorescent imaging, 01 natural sciences, active plasmonics, super-resolution microscopy, DNA origami, Physics - Biological Physics, Electrical and Electronic Engineering, fluorescence enhancement, molecular self-assembly, 021001 nanoscience & nanotechnology, Atomic and Molecular Physics, and Optics, 0104 chemical sciences, Electronic, Optical and Magnetic Materials, Template, Nanolithography, Biological Physics (physics.bio-ph), plasmon coupling, 0210 nano-technology, Physics - Optics, Biotechnology, Optics (physics.optics)
Abstract: The specificity and simplicity of the Watson–Crick base pair interactions make DNA one of the most versatile construction materials for creating nanoscale structures and devices. Among several DNA-based approaches, the DNA origami technique excels in programmable self-assembly of complex, arbitrary shaped structures with dimensions of hundreds of nanometers. Importantly, DNA origami can be used as templates for assembly of functional nanoscale components into three-dimensional structures with high precision and controlled stoichiometry. This is often beyond the reach of other nanofabrication techniques. In this Perspective, we highlight the capability of the DNA origami technique for realization of novel nanophotonic systems. First, we introduce the basic principles of designing and fabrication of DNA origami structures. Subsequently, we review recent advances of the DNA origami applications in nanoplasmonics, single-molecule and super-resolution fluorescent imaging, as well as hybrid photonic systems. We conclude by outlining the future prospects of the DNA origami technique for advanced nanophotonic systems with tailored functionalities.
Published: 2021
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32. Safety and recommendations for TMS use in healthy subjects and patient populations, with updates on training, ethical and regulatory issues

Author: Rossi, Simone, Antal, Andrea, Bestmann, Sven, Bikson, Marom, Brewer, Carmen, Brockmöller, Jürgen, Carpenter, Linda L., Cincotta, Massimo, Chen, Robert, Daskalakis, Jeff D., Di Lazzaro, Vincenzo, Fox, Michael D., George, Mark S., Gilbert, Donald, Kimiskidis, Vasilios K., Koch, Giacomo, Ilmoniemi, Risto J., Pascal Lefaucheur, Jean, Leocani, Letizia, Lisanby, Sarah H., Miniussi, Carlo, Padberg, Frank, Pascual-Leone, Alvaro, Paulus, Walter, Peterchev, Angel V., Quartarone, Angelo, Rotenberg, Alexander, Rothwell, John, Rossini, Paolo M., Santarnecchi, Emiliano, Shafi, Mouhsin M., Siebner, Hartwig R., Ugawa, Yoshikatzu, Wassermann, Eric M., Zangen, Abraham, Ziemann, Ulf, Hallett, Mark, University of Siena, University of Göttingen, University College London, City University of New York, National Institutes of Health, Brown University, Central Tuscany Local Health Authority, University of Toronto, Universita Campus Bio-Medico di Roma, Harvard Medical School, Medical University of South Carolina, Cincinnati Children's Hospital Medical Center, Aristotle University of Thessaloniki, IRCCS Fondazione Santa Lucia - Roma, Department of Neuroscience and Biomedical Engineering, Université Paris-Est Créteil, Vita-Salute San Raffaele University, BEC-INFM, Ludwig Maximilian University of Munich, Children's Healthcare of Atlanta, Duke University, University of Messina, IRCCS San Raffaele Pisana - Roma, University of Copenhagen, Fukushima Medical University, Ben-Gurion University of the Negev, University of Tübingen, Aalto-yliopisto, and Aalto University
Subjects: Psychiatry, Neurology, Neuromodulation, TMS, rTMS, TBS, Safety, QPS
Abstract: This article is based on a consensus conference, promoted and supported by the International Federation of Clinical Neurophysiology (IFCN), which took place in Siena (Italy) in October 2018. The meeting intended to update the ten-year-old safety guidelines for the application of transcranial magnetic stimulation (TMS) in research and clinical settings (Rossi et al., 2009). Therefore, only emerging and new issues are covered in detail, leaving still valid the 2009 recommendations regarding the description of conventional or patterned TMS protocols, the screening of subjects/patients, the need of neurophysiological monitoring for new protocols, the utilization of reference thresholds of stimulation, the managing of seizures and the list of minor side effects. New issues discussed in detail from the meeting up to April 2020 are safety issues of recently developed stimulation devices and pulse configurations; duties and responsibility of device makers; novel scenarios of TMS applications such as in the neuroimaging context or imaging-guided and robot-guided TMS; TMS interleaved with transcranial electrical stimulation; safety during paired associative stimulation interventions; and risks of using TMS to induce therapeutic seizures (magnetic seizure therapy). An update on the possible induction of seizures, theoretically the most serious risk of TMS, is provided. It has become apparent that such a risk is low, even in patients taking drugs acting on the central nervous system, at least with the use of traditional stimulation parameters and focal coils for which large data sets are available. Finally, new operational guidelines are provided for safety in planning future trials based on traditional and patterned TMS protocols, as well as a summary of the minimal training requirements for operators, and a note on ethics of neuroenhancement.
Published: 2021

33. Structural inventory of native ribosomal ABCE1-43S pre-initiation complexes

Author: Micheline Fromont-Racine, Otto Berninghausen, Michael Ameismeier, Abdelkader Namane, Roland Beckmann, Estelle Dacheux, Thomas Becker, Jingdong Cheng, Timur Mackens-Kiani, Hanna Kratzat, and Gene Center, Ludwig Maximilian University of Munich, 81377 Munich, Germany
Subjects: 0303 health sciences, eIF2, biology, Chemistry, Protein subunit, [SDV]Life Sciences [q-bio], Context (language use), 03 medical and health sciences, ABCE1, 0302 clinical medicine, Eukaryotic translation, Biochemistry, Transfer RNA, biology.protein, Eukaryotic Small Ribosomal Subunit, Ternary complex, 030217 neurology & neurosurgery, 030304 developmental biology
Abstract: In eukaryotic translation, the termination and recycling phases are linked to subsequent initiation by persistence of several factors. These comprise the large eIF3 complex, eIF3j (Hcr1 in yeast) and the ATP-binding cassette protein ABCE1 (Rli1 in yeast). The ATPase is mainly active as a recycling factor, but it can remain bound to the dissociated 40S subunit until formation of 43S pre-initiation complexes. However, its functional role and native architectural context remains largely enigmatic. Here, we present an architectural inventory of native yeast and human ABCE1-containing pre-initiation complexes by cryo-EM. We found that ABCE1 was mostly associated with early 43S but also later 48S phases of initiation. It directly interacted with eIF3j via its unique iron-sulfur cluster domain and adopted a novel hybrid conformation, which was ATPase-inhibited and stabilized by an unknown factor bound between the nucleotide binding sites. Moreover, the native human samples provided a near-complete molecular picture of the architecture and sophisticated interaction network of the 43S-bound eIF3 complex and also the eIF2 ternary complex containing the initiator tRNA.
Published: 2020

34. Dust-to-Gas and Refractory-to-Ice Mass Ratios of Comet 67P/Churyumov-Gerasimenko from Rosetta Observations

Author: Ekkehard Kührt, Nicolas Biver, Dominique Bockelée-Morvan, Raphael Marschall, Mathieu Choukroun, Matthew Taylor, Nicolas Thomas, Kathrin Altwegg, Martin Hilchenbach, Martin Pätzold, Alain Herique, Joanna Drążkowska, Jet Propulsion Laboratory (JPL), NASA-California Institute of Technology (CALTECH), Physikalisches Institut [Bern], Universität Bern [Bern], DLR Institut für Planetenforschung, Deutsches Zentrum für Luft- und Raumfahrt [Berlin] (DLR), Laboratoire d'études spatiales et d'instrumentation en astrophysique (LESIA), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire de Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'études spatiales et d'instrumentation en astrophysique (LESIA (UMR_8109)), Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire de Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Ludwig Maximilian University of Munich [Germany] (LMU München), Institut de Planétologie et d'Astrophysique de Grenoble (IPAG), Centre National d'Études Spatiales [Toulouse] (CNES)-Observatoire des Sciences de l'Univers de Grenoble (OSUG ), Institut national des sciences de l'Univers (INSU - CNRS)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA), Max-Planck-Institut für Sonnensystemforschung (MPS), Max-Planck-Gesellschaft, Rhenish Institute for Environmental Research (RIU), University of Cologne, RSSD of ESA, ESTEC, Noordwijk, The Netherlands, and Physics Institute, University of Bern, 3012 Bern, Switzerland
Subjects: Physics, 67P/Churyumov-Gerasimenko, 010504 meteorology & atmospheric sciences, [SDU.ASTR]Sciences of the Universe [physics]/Astrophysics [astro-ph], 520 Astronomy, refractory-to-ice ratio, Comet, Astronomy and Astrophysics, Astrophysics, Mass ratio, 620 Engineering, 01 natural sciences, Planetary science, Space and Planetary Science, [SDU]Sciences of the Universe [physics], 0103 physical sciences, Rosetta, Comets, Refractory-to-Ice mass ratio, Single point, Dust-to-gas ratio, 010303 astronomy & astrophysics, Institut für Optische Sensorsysteme, Refractory (planetary science), 0105 earth and related environmental sciences
Abstract: This chapter reviews the estimates of the dust-to-gas and refractory-to-ice mass ratios derived fromRosettameasurements in the lost materials and the nucleus of 67P/Churyumov-Gerasimenko, respectively. First, the measurements byRosettainstruments are described, as well as relevant characteristics of 67P. The complex picture of the activity of 67P, with its extreme North-South seasonal asymmetry, is presented. Individual estimates of the dust-to-gas and refractory-to-ice mass ratios are then presented and compared, showing wide ranges of plausible values.Rosetta’s wealth of information suggests that estimates of the dust-to-gas mass ratio made in cometary comae at a single point in time may not be fully representative of the refractory-to-ice mass ratio within the cometary nuclei being observed.
Published: 2020

35. Divergent Evolution of Legionella RCC1 Repeat Effectors Defines the Range of Ran GTPase Cycle Targets

Author: Swart, A Leoni, Steiner, Bernhard, Gomez-Valero, Laura, Schütz, Sabina, Hannemann, Mandy, Janning, Petra, Irminger, Michael, Rothmeier, Eva, Buchrieser, Carmen, Itzen, Aymelt, Panse, Vikram Govind, Hilbi, Hubert, Institute of Medical Microbiology [Zurich], Universität Zürich [Zürich] = University of Zurich (UZH), Biologie des Bactéries intracellulaires - Biology of Intracellular Bacteria, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Center for Integrated Protein Science (CIPSM), Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM)-Helmholtz-Zentrum München (HZM)-Ludwig Maximilian University of Munich [Germany] (LMU München), Max-Planck-Institut für Molekulare Physiologie, Max-Planck-Gesellschaft, Max Von Pettenkofer Institute (MVP), Ludwig-Maximilians-Universität München (LMU), Institute of Biochemistry and Signal Transduction [Hamburg, Germany], Universitaetsklinikum Hamburg-Eppendorf = University Medical Center Hamburg-Eppendorf [Hamburg] (UKE), Research in the laboratory of H.H. was supported by the Swiss National Science Foundation (SNF, 31003A_153200 and 31003A_175557), the OPO Foundation, and the Novartis Foundation for Medical-Biological Research. A. Welin was supported by a grant from the Swedish Research Council (2014-396). V.G.P. was supported by grants from the Swiss National Science Foundation, NCCR RNA & Disease, Novartis Foundation for Medical-Biological Research and the Olga Mayenfisch Foundation., Work in the laboratory of C.B. was financed by the Institut Pasteur and by grant ANR-10-LABX-62-IBEID., We thank Amanda Welin for performing imaging flow cytometry experiments and Nadia Keller for flow cytometry analysis., Confocal laser scanning microscopy and imaging flow cytometry were performed using equipment of the Center of Microscopy and Image Analysis, University of Zurich. Proteomics analysis was performed at the Functional Genomics Center Zürich., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), University of Zurich, Hilbi, Hubert, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), and Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM)-Ludwig-Maximilians-Universität München (LMU)-Helmholtz Zentrum München = German Research Center for Environmental Health
Subjects: 10179 Institute of Medical Microbiology, 2404 Microbiology, Legionella, 610 Medicine & health, Acanthamoeba, macrophage, host-pathogen interaction, bacterial evolution, Microbiology, type IV secretion, QR1-502, phosphoinositide lipid, small GTPase, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], vesicle trafficking, 2406 Virology, 570 Life sciences, biology, guanine nucleotide exchange factor, Dictyostelium, amoeba, effector protein, microtubule, pathogen vacuole
Abstract: International audience; Legionella pneumophila governs its interactions with host cells by secreting >300 different "effector" proteins. Some of these effectors contain eukaryotic domains such as the RCC1 (regulator of chromosome condensation 1) repeats promoting the activation of the small GTPase Ran. In this report, we reveal a conserved pattern of L. pneumophila RCC1 repeat genes, which are distributed in two main clusters of strains. Accordingly, strain Philadelphia-1 contains two RCC1 genes implicated in bacterial virulence, legG1 (Legionella eukaryotic gene 1), and ppgA, while strain Paris contains only one, pieG. The RCC1 repeat effectors localize to different cellular compartments and bind distinct components of the Ran GTPase cycle, including Ran modulators and the small GTPase itself, and yet they all promote the activation of Ran. The pieG gene spans the corresponding open reading frames of legG1 and a separate adjacent upstream gene, lpg1975. legG1 and lpg1975 are fused upon addition of a single nucleotide to encode a protein that adopts the binding specificity of PieG. Thus, a point mutation in pieG splits the gene, altering the effec-tor target. These results indicate that divergent evolution of RCC1 repeat effectors defines the Ran GTPase cycle targets and that modulation of different components of the cycle might fine-tune Ran activation during Legionella infection.IMPORTANCE Legionella pneumophila is a ubiquitous environmental bacterium which, upon inhalation, causes a life-threatening pneumonia termed Legionnaires' disease. The opportunistic pathogen grows in amoebae and macrophages by employing a "type IV" secretion system, which secretes more than 300 different "effec-tor" proteins into the host cell, where they subvert pivotal processes. The function of many of these effector proteins is unknown, and their evolution has not been studied. L. pneumophila RCC1 repeat effectors target the small GTPase Ran, a molecular switch implicated in different cellular processes such as nucleocytoplasmic transport and microtubule cytoskeleton dynamics. We provide evidence that one or more RCC1 repeat genes are distributed in two main clusters of L. pneumophila strains and have divergently evolved to target different components of the Ran GTPase activation cycle at different subcellular sites. Thus, L. pneumophila employs a sophisticated strategy to subvert host cell Ran GTPase during infection.
Published: 2020

36. A real-time cell-binding assay reveals dynamic features of STxB-Gb3 cointernalization and STxB-mediated cargo delivery into cancer cells

Author: Grzegorz M Popowicz, Jos Buijs, Valeria Napolitano, Giulia Opassi, U. Helena Danielson, Hanna Björkelund, Grzegorz Dubin, João Crispim Encarnação, Hélène Munier-Lehmann, Karl Andersson, Ridgeview Instruments AB [Uppsala, Sueden], Department of Immunology, Genetics and Pathology [Uppsala, Sueden] (IGP), Uppsala University, Uniwersytet Jagielloński w Krakowie = Jagiellonian University (UJ), Department of Chemistry [Uppsala, Sueden], Biomedical Center = Biomedicinskt centrum [Uppsala, Sueden] (BMC), Uppsala University-Uppsala University, Malopolska Centre of Biotechnology [Krakow] (MCB), Helmholtz-Zentrum München (HZM), Center for Integrated Protein Science (CIPSM), Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM)-Helmholtz-Zentrum München (HZM)-Ludwig Maximilian University of Munich [Germany] (LMU München), Chimie et Biocatalyse, Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], This project has received funding from the European Union's Framework Programme for Research and Innovation Horizon 2020 (2014–2020) under the Marie Sklodowska‐Curie Grant Agreement No. 675555, Accelerated Early staGe drug discovery (AEGIS)., Microscopic imaging was performed with equipment maintained by the BioVis Platform at Uppsala University. We thank to Dr Arie Geerlof and Dr André Mourão (Institute of Structural Biology, Helmholtz Zentrum München) for providing help on protein expression and purification of the STxB constructs., European Project: 675555,H2020,H2020-MSCA-ITN-2015,AEGIS(2016), Helmholtz Zentrum München = German Research Center for Environmental Health, Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM)-Ludwig-Maximilians-Universität München (LMU)-Helmholtz Zentrum München = German Research Center for Environmental Health, and Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)
Subjects: media_common.quotation_subject, [SDV]Life Sciences [q-bio], Cell, education, Biophysics, Globotriaosylceramide, cell surface receptor, Biological Transport, Active, Shiga Toxins, Biochemistry, real-time cell-binding assays, 03 medical and health sciences, chemistry.chemical_compound, [SPI]Engineering Sciences [physics], Structural Biology, Cell surface receptor, receptor internalization, Neoplasms, Genetics, medicine, binding kinetics, cancer, Humans, Internalization, Molecular Biology, Binding Kinetics, Cancer, Cell Surface Receptor, Real-time Cell-binding Assays, Receptor Internalization, Shiga Toxin, real-time cell- binding assays, 030304 developmental biology, media_common, 0303 health sciences, Drug Carriers, Chemistry, Trihexosylceramides, 030302 biochemistry & molecular biology, Biochemistry and Molecular Biology, Cell Biology, Shiga toxin, Small molecule, Receptor–ligand kinetics, 3. Good health, Cell biology, ddc, medicine.anatomical_structure, Cancer cell, Biological Assay, K562 Cells, HT29 Cells, Intracellular, Biokemi och molekylärbiologi
Abstract: International audience; The interaction between the Shiga toxin B-subunit (STxB) and its globotriaosylceramide receptor (Gb3) has a high potential for being exploited for targeted cancer therapy. The primary goal of this study was to evaluate the capacity of STxB to carry small molecules and proteins as cargo into cells. For this purpose, an assay was designed to provide real-time information about the StxB-Gb3 interaction as well as the dynamics and mechanism of the internalization process. The assay revealed the ability to distinguish the process of binding to the cell surface from internalization and presented the importance of receptor and STxB clustering for internalization. The overall setup demonstrated that the binding mechanism is complex, and the concept of affinity is difficult to apply. Hence, time-resolved methods, providing detailed information about the interaction of STxB with cells, are critical for the optimization of intracellular delivery.
Published: 2020

37. Tailored algorithms for the detection of the atmospheric boundary layer height from common automatic lidars and ceilometers (Alc)

Author: Matthias Wiegner, Maxime Hervo, Yann Poltera, Martial Haeffelin, Sue Grimmond, Alexander Haefele, Jean Charles Dupont, Marc Antoine Drouin, Simone Kotthaus, Laboratoire de Météorologie Dynamique (UMR 8539) (LMD), Institut national des sciences de l'Univers (INSU - CNRS)-École polytechnique (X)-École des Ponts ParisTech (ENPC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Département des Géosciences - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), DEPARTMENT OF METEOROLOGY UNIVERSITY OF READING GBR, University of Reading (UOR), Federal Office of Meteorology and Climatology MeteoSwiss, Institute for Atmospheric and Climate Science [Zürich] (IAC), Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich), Ludwig Maximilian University of Munich [Germany] (LMU München), EP/P002331/1 Centre National d’Etudes Spatiales, CNES École Polytechnique Fédérale de Lausanne, EPFL Met Office, and Funding: This research was funded by ACTRIS-2 and EUMETNET through the E-PROFILE program. SIRTA is supported by CNES, École Polytechnique, INSU-CNRS, and IPSL. Contribution of SG funded by EPSRC DARE (EP/P002331/1) and Met Office.
Subjects: Daytime, Ceilometer, Lidar, 010504 meteorology & atmospheric sciences, Planetary boundary layer, Science, Context (language use), 010501 environmental sciences, Future climate, E-PROFILE, 01 natural sciences, Boundary layer height, Deep convection, 13. Climate action, [SDE]Environmental Sciences, General Earth and Planetary Sciences, Environmental science, ALC network, boundary layer height, lidar, ceilometer, Pollution dispersion, Algorithm, 0105 earth and related environmental sciences
Abstract: International audience; A detailed understanding of atmospheric boundary layer (ABL) processes is key to improve forecasting of pollution dispersion and cloud dynamics in the context of future climate scenarios. International networks of automatic lidars and ceilometers (ALC) are gathering valuable data that allow for the height of the ABL and its sublayers to be derived in near real time. A new generation of advanced methods to automatically detect the ABL heights now exist. However, diversity in ALC models means these algorithms need to be tailored to instrument-specific capabilities. Here, the advanced algorithm STRATfinder is presented for application to high signal-to-noise ratio (SNR) ALC observations, and results are compared to an automatic algorithm designed for low-SNR measurements (CABAM). The two algorithms are evaluated for application in an operational network setting. Results indicate that the ABL heights derived from low-SNR ALC have increased uncertainty during daytime deep convection, while high-SNR observations can have slightly reduced capabilities in detecting shallow nocturnal layers. Agreement between the ALC-based methods is similar when either is compared to the ABL heights derived from temperature profile data. The two independent methods describe very similar average diurnal and seasonal variations. Hence, high-quality products of ABL heights may soon become possible at national and continental scales.
Published: 2020

38. Comparison of four PCR methods for efficient detection of Trypanosoma cruzi in routine diagnostics

Author: Michael Pritsch, Thomas Löscher, Edoardo Marchisio, Stefan Hohnerlein, Carolin Mengele, Gisela Bretzel, Kerstin Helfrich, Peter Seiringer, Nicole Berens-Riha, Michael Hoelscher, María Flores-Chávez, German Center for Infection Research, University of Munich, German Center for Infection Research (Alemania), and Ludwig Maximilian University of Munich (Alemania)
Subjects: Adult, Male, 0301 basic medicine, Microbiology (medical), Chagas disease, Conventional, Adolescent, Trypanosoma cruzi, 030231 tropical medicine, 030106 microbiology, Comparison, Minicircle, Polymerase Chain Reaction, Sensitivity and Specificity, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Diagnosis, parasitic diseases, medicine, Humans, Chagas Disease, Positive serology, biology, General Medicine, Middle Aged, biology.organism_classification, medicine.disease, Leishmania, Trypanosoma cruzi DNA, PCR, Blood, Infectious Diseases, Molecular Diagnostic Techniques, Child, Preschool, Immunology, biology.protein, Female, Pcr method, Antibody, Real-time
Abstract: Due to increased migration, Chagas disease has become an international health problem. Reliable diagnosis of chronically infected people is crucial for prevention of non-vectorial transmission as well as treatment. This study compared four distinct PCR methods for detection of Trypanosoma cruzi DNA for the use in well-equipped routine diagnostic laboratories. DNA was extracted of T. cruzi-positive and negative patients' blood samples and cultured T. cruzi, T. rangeli as well as Leishmania spp. One conventional and two real-time PCR methods targeting a repetitive Sat-DNA sequence as well as one conventional PCR method targeting the variable region of the kDNA minicircle were compared for sensitivity, intra- and interassay precision, limit of detection, specificity and cross-reactivity. Considering the performance, costs and ease of use, an algorithm for PCR-diagnosis of patients with a positive serology for T. cruzi antibodies was developed. This research was supported by the German Center for InfectionResearch through the MD program (to MH, MP and PS). Overall,the project wasfinanced by the University of Munich (LMU). Sí
Published: 2017

39. The Clothes Swapping Phenomenon:When Consumers become Suppliers

Author: Kirsi Niinimäki, Nina Bürklin, Claudia E. Henninger, University of Manchester, Ludwig Maximilian University of Munich, Department of Design, Aalto-yliopisto, and Aalto University
Subjects: Fashion industry, Marketing, Supply chain management, business.industry, Supply chain, 05 social sciences, Business model, Clothing, Swapping, Sharing economy, Blueprint, Collaborative consumption, Phenomenon, Suppliers, 0502 economics and business, Swapping, collaborative consumption, fashion industry, supply chain, consumers, suppliers, Consumers, 050211 marketing, Business, Business and International Management, 050203 business & management
Abstract: PurposeThe purpose of this paper is to explore swap-shops, which emerged as part of the collaborative consumption phenomenon, by investigating what the implications are of consumers acting as suppliers and how this affects supply chain management within the context of the fashion industry.Design/methodology/approachThis study explores the collaborative consumption phenomenon through swap-shops in three countries: the UK, Finland and Germany. In-depth semi-structured interviews were conducted with swappers, non-swappers and organisers. To further enhance the data set six observations of swap-shop events were conducted. Data were transcribed and analysed using multiple coding cycles and using a grounded research approach.FindingsFindings indicate that consumers were most concerned with availability/sizing and quality of garments, whilst organisers felt uncertainty was the biggest issue. Data allowed creating a framework that blueprints the swapping supply chain, in which consumers emerge as suppliers. It highlights possible activities in different cycles, whilst furthermore indicates that consumption cycles can move from monetary (e.g. selling) to non-monetary transactions (e.g. swapping) and vice versa.Practical implicationsSwapping as a relatively new fashion supply mode implies a fluidity of market roles. Disruptive business models can blur boundaries between the supply- and demand-side. This indicates that consumers can change “roles” multiple times as they go through the consumption cycle.Originality/valueThe authors extended the knowledge on swapping by describing how this phenomenon can activate consumers, and extend and intensify the use of garments and therefore swapping can slow the material throughput in the system. It is the first paper to focus solely on swapping within a three country context.
Published: 2019

40. Directional Threading and Sliding of a Dissymmetrical Foldamer Helix on Dissymmetrical Axles

Author: Xiang Wang, Ivan Huc, Barbara Wicher, Quan Gan, Yann Ferrand, Chimie et Biologie des Membranes et des Nanoobjets (CBMN), Université de Bordeaux (UB)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Poznan University of Medical Sciences [Poland] (PUMS), Center for Integrated Protein Science (CIPSM), Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM)-Helmholtz-Zentrum München (HZM)-Ludwig Maximilian University of Munich [Germany] (LMU München), and Université Sciences et Technologies - Bordeaux 1-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Materials science, 010405 organic chemistry, Supramolecular chemistry, Foldamer, molecular shuttle, General Medicine, General Chemistry, [CHIM.MATE]Chemical Sciences/Material chemistry, [CHIM.INOR]Chemical Sciences/Inorganic chemistry, 010402 general chemistry, 01 natural sciences, Catalysis, 0104 chemical sciences, Crystallography, Molecular recognition, Wrap around, Proton NMR, Molecule, [CHIM]Chemical Sciences, foldaxane, Dumbbell, molecular recognition, Threading (protein sequence), directional motion
Abstract: International audience; We have investigated the self-assembly of a dissymmetrical aromatic oligoamide helix on linear amido-carbamate rods. A dissymmetric sequence bearing two differentiated ends is able to wrap around dissymmetric dumbbell guest molecules. Structural and thermodynamic investigations allowed us to decipher the mode of binding of the helix that can bind specifically to the amide and carbamate groups of the rod. In parallel kinetic studies of threading and sliding of the helix along linear axles were also monitored by 1 H NMR. Results show that threading of a dissymmetrical host can be kinetically biased by the nature of the guest terminus allowing a preferential sense of sliding of the helix. The study presented below further demonstrates the valuable potential of foldaxanes to combine designed molecular recognition patterns with fine control of self-assembly kinetics to conceive complex supramolecular events.
Published: 2019

41. Biodiversity of Environmental Leptospira: Improving Identification and Revisiting the Diagnosis

Author: Dominique Girault, Gregorio Iraola, Roman Thibeaux, Anna Rettinger, Mathieu Picardeau, Emilie Bierque, Cyrille Goarant, Anthony Douyère, Marie-Estelle Soupé-Gilbert, Michael Meyer, Unité de Recherche et d'Expertise Leptospirose - Leptospirosis Research and Expertise Unit [Nouméa, Nouvelle-Calédonie] (UREL), Institut Pasteur de Nouvelle-Calédonie, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Institute for Infectious Diseases and Zoonoses (Faculty of Veterinary Medecine), Ludwig Maximilian University of Munich [Germany] (LMU München), Institut de sciences exactes et appliquées (ISEA), Université de la Nouvelle-Calédonie (UNC), Bioinformatics / Bioinformática [Montevideo], Institut Pasteur de Montevideo, Biologie des Spirochètes / Biology of Spirochetes, Institut Pasteur [Paris], This work was supported by a PTR (grant 30-17) from the Institut Pasteur (Paris). The researcher position of RT was supported in 2015-2017 by an AXA Research Funds grant AXA Postdoctoral Fellowship 15-AXA-PDOC-037 and by the Government of New Caledonia since 2018. EB has a doctoral grant from the Institut Pasteur International Network. GI is supported by the Fondo de Convergencia Estructural del Mercosur (FOCEM) grant COF 04/11. Thanks are due to Emilie Barsac, Benjamin De Georges De Ledenon and Julien Colot, for technical assistance with the MALDI-ToF and to Vincent Enouf and his platform team [Institut Pasteur, Pasteur International Bioresources network (PIBnet), Mutualized Platform for Microbiology (P2M)] for the next-generation sequencing analysis. Thanks are also due to the CRESICA [Consortium for Research, Higher Education and Innovation in New Caledonia] for funding the SEM platform. Leptospira collection permits were obtained from the North (# 60912-2002-2017/JJC) and South (Arrêté 1689-2017/ARR/DENV) Provinces of New Caledonia., Ludwig-Maximilians-Universität München (LMU), and Institut Pasteur [Paris] (IP)
Subjects: 0301 basic medicine, Microbiology (medical), animal diseases, 030106 microbiology, Biodiversity, lcsh:QR1-502, Computational biology, Diagnostic tools, Microbiology, lcsh:Microbiology, 03 medical and health sciences, Species level, Leptospira, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, medicine, Species identification, MALDI-TOF MS, Original Research, MALDI-ToF, WGS comparisons, biology, biology.organism_classification, Isolation (microbiology), medicine.disease, bacterial infections and mycoses, Leptospirosis, Novel species, 3. Good health, Bacterial isolation, Soil microbiology, bacteria, Identification (biology), isolation
Abstract: International audience; Leptospirosis is an important environmental disease and a major threat to human health causing at least 1 million clinical infections annually. There has recently been a growing interest in understanding the environmental lifestyle of Leptospira. However, Leptospira isolation from complex environmental samples is difficult and time-consuming and few tools are available to identify Leptospira isolates at the species level. Here, we propose a polyphasic isolation and identification scheme, which might prove useful to recover and identify environmental isolates and select those to be submitted to whole-genome sequencing. Using this approach, we recently described 12 novel Leptospira species for which we propose names. We also show that MALDI-ToF MS allows rapid and reliable identification and provide an extensive database of Leptospira MALDI-ToF mass spectra, which will be valuable to researchers in the leptospirosis community for species identification. Lastly, we also re-evaluate some of the current techniques for the molecular diagnosis of leptospirosis taking into account the extensive and recently revealed biodiversity of Leptospira in the environment. In conclusion, we describe our method for isolating Leptospira from the environment, confirm the usefulness of mass spectrometry for species identification and propose names for 12 novel species. This also offers the opportunity to refine current molecular diagnostic tools.
Published: 2018

42. Physical keyboards in Virtual reality

Author: Knierim, Pascal, Schwind, Valentin, Feit, Anna Maria, Nieuwenhuizen, Florian, Henze, Niels, Ludwig Maximilian University of Munich, University of Stuttgart, Department of Communications and Networking, Aalto-yliopisto, and Aalto University
Subjects: Physical keyboard, Virtual Reality, Hands, Text entry
Abstract: Entering text is one of the most common tasks when interacting with computing systems. Virtual Reality (VR) presents a challenge as neither the user's hands nor the physical input devices are directly visible. Hence, conventional desktop peripherals are very slow, imprecise, and cumbersome. We developed a apparatus that tracks the user's hands, and a physical keyboard, and visualize them in VR. In a text input study with 32 participants, we investigated the achievable text entry speed and the effect of hand representations and transparency on typing performance, workload, and presence. With our apparatus, experienced typists benefited from seeing their hands, and reach almost outside-VR performance. Inexperienced typists profited from semi-transparent hands, which enabled them to type just 5.6 WPM slower than with a regular desktop setup. We conclude that optimizing the visualization of hands in VR is important, especially for inexperienced typists, to enable a high typing performance.
Published: 2018

43. Syntheses and Crystal Structures of Sodium Hydrogen Fluorides NaF·nHF (n = 2, 3, 4)

Author: Martin J. Mühlbauer, Antti J. Karttunen, Andreas Kornath, Florian Kraus, Sergei I. Ivlev, Theresa Soltner, University of Marburg, Ludwig Maximilian University of Munich, Department of Chemistry and Materials Science, Technical University of Munich, Aalto-yliopisto, and Aalto University
Subjects: Hydrogen, 010405 organic chemistry, Hydrogen bond, Sodium, Crystal structure, Neutron diffraction, chemistry.chemical_element, Infrared spectroscopy, 010402 general chemistry, Sodium hydrogen fluorides, 01 natural sciences, 0104 chemical sciences, Hydrogen bonds, Inorganic Chemistry, Crystallography, chemistry.chemical_compound, Trifluoride, Density functional calculations, chemistry, IR spectroscopy, Tetrafluoride, ta116
Abstract: A series of higher sodium hydrogen fluorides were synthesized and structurally characterized. Sodium tetrahydrogen pentafluoride, NaH4F5, crystallizes in space group type I4(1)/a with cell parameters a = 6.0275(4), c = 11.6208(13) angstrom, V = 422.19(7) angstrom(3), Z = 4 at 100 K. Sodium trihydrogen tetrafluoride, NaH3F4, crystallizes in space group type R (3) over bar with cell parameters a = 6.5139(4), c = 13.4040(15) angstrom, V = 492.55(8) angstrom(3), Z = 6 at 100 K. Sodium dihydrogen trifluoride, NaH2F3, crystallizes in space group type Pnma with cell parameters a = 7.9276(9), b = 3.4514(3), c = 9.6937(10) angstrom, V = 265.23(5) angstrom(3), Z = 4 at 100 K. The investigations were complemented by IR spectroscopy and theoretical calculations. NaH2F3 was additionally characterized by means of powder neutron diffraction. All observed F-H center dot center dot center dot F hydrogen bonds are unsymmetric, close to linearity and can be considered as strong hydrogen bonds.
Published: 2017

44. CHRAC/ACF Contribute to the Repressive Ground State of Chromatin

Author: Xu Zhang, Dhawal Jain, Frank Schnorrer, Alessandro Scacchetti, Peter B. Becker, Bas van Steensel, Laura Brueckner, Tamas Schauer, Tobias Straub, Center for Integrated Protein Science (CIPSM), Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM)-Ludwig-Maximilians-Universität München (LMU)-Helmholtz Zentrum München = German Research Center for Environmental Health, Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut de Biologie du Développement de Marseille (IBDM), Aix Marseille Université (AMU)-Collège de France (CdF (institution))-Centre National de la Recherche Scientifique (CNRS), U54 DK107965, National Institutes of Health, MSCA-ITN-2014-ETN No. 642934, European Research Council (ERC), FP/2007–2013, ERC, CNRSExcellence Initiative Aix-Marseille University AMIDEXANRLabEX-INFORM, Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM)-Helmholtz-Zentrum München (HZM)-Ludwig Maximilian University of Munich [Germany] (LMU München), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), and Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)
Subjects: 0301 basic medicine, Health, Toxicology and Mutagenesis, [SDV]Life Sciences [q-bio], Plant Science, Biology, Biochemistry, Genetics and Molecular Biology (miscellaneous), Chromatin remodeling, Transcriptome, 03 medical and health sciences, 0302 clinical medicine, Transcription (biology), Gene expression, Nucleosome, Gene, ChIA-PET, Research Articles, 030304 developmental biology, Genetics, 0303 health sciences, Reporter gene, Ecology, biology.organism_classification, Chromatin, Cell biology, 030104 developmental biology, Drosophila melanogaster, 030217 neurology & neurosurgery, Research Article
Abstract: Chromatin accessibility complex/ATP-utilizing chromatin assembly and remodeling factor help to establish basal transcriptional repression, conceivably through improving the regular spacing of nucleosomes in euchromatin., The chromatin remodeling complexes chromatin accessibility complex and ATP-utilizing chromatin assembly and remodeling factor (ACF) combine the ATPase ISWI with the signature subunit ACF1. These enzymes catalyze well-studied nucleosome sliding reactions in vitro, but how their actions affect physiological gene expression remains unclear. Here, we explored the influence of Drosophila melanogaster chromatin accessibility complex/ACF on transcription by using complementary gain- and loss-of-function approaches. Targeting ACF1 to multiple reporter genes inserted at many different genomic locations revealed a context-dependent inactivation of poorly transcribed reporters in repressive chromatin. Accordingly, single-embryo transcriptome analysis of an Acf knock-out allele showed that only lowly expressed genes are derepressed in the absence of ACF1. Finally, the nucleosome arrays in Acf-deficient chromatin show loss of physiological regularity, particularly in transcriptionally inactive domains. Taken together, our results highlight that ACF1-containing remodeling factors contribute to the establishment of an inactive ground state of the genome through chromatin organization.
Published: 2017
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45. Planck intermediate results

Author: Ade, P. A. R., Aghanim, N., Arnaud, M., Ashdown, M., Aubourg, E., Aumont, J., Baccigalupi, C., Banday, A. J., Barreiro, R. B., Bartolo, N., Battaner, E., Benabed, K., Benoit-Levy, A., Bersanelli, M., Bielewicz, P., Bock, J. J., Bonaldi, A., Bonavera, L., Bond, J. R., Borrill, J., Bouchet, F. R., Burigana, C., Calabrese, E., Cardoso, J. -F., Catalano, A., Chamballu, A., Chiang, H. C., Christensen, P. R., Clements, D. L., Colombo, L. P. L., Combet, C., Crill, B. P., Curto, A., Cuttaia, F., Danese, L., Davies, R. D., Davis, R. J., de Bernardis, P., de Zotti, G., Delabrouille, J., Dickinson, C., Diego, J. M., Dolag, K., Donzelli, S., Doré, O., Douspis, M., Ducout, A., Dupac, X., Leon-Tavares, J., Savelainen, M., Planck Collaboration, University of Oslo, University of Cambridge, Université Paris-Sud, International School for Advanced Studies, University of Bologna, CNRS, CSIC, University of Padova, Institut d 'Astrophysique de Paris, University of Milan, University of Oviedo, University of Toronto, Lawrence Berkeley National Laboratory, Observatoire de Paris, University of Ferrara, Istituto di Astrofisica Spaziale e Fisica Cosmica di Bologna, University of Oxford, University of Sussex, University of KwaZulu-Natal, University of Southern California, Université Grenoble Alpes, California Institute of Technology, Sapienza University of Rome, Astronomical Observatory of Padua, University of Manchester, Imperial College London, European Space Astronomy Centre, University College London, Max Planck Institute for Astrophysics, African Institute for Mathematical Sciences, Jodrell Bank Centre for Astrophysics, Topological Quantum Fluids, Department of Applied Physics, Aalto-yliopisto, Aalto University, Cardiff University, Metsähovi Radio Observatory, University of Helsinki, Université de Toulouse, Universidad de Cantabria, Instituto Nationale di Fisica Nucleare, University of Granada, Pierre and Marie Curie University, Istituto Nazionale Astrofisica - Italy, University of California Berkeley, Telecom ParisTech, University of Paris Diderot, Princeton University, Niels Bohr Institute, University of Copenhagen, Department of Radio Science and Engineering, Université Paris Diderot, Kavli Institute for Cosmology Cambridge, Université Fédérale Toulouse Midi-Pyrénées, Università degli Studi di Milano, Nicolaus Copernicus Astronomical Center, INAF/IASF Bologna, Niels Bohr Institute, INAF, Osservatorio Astronomico di Padova, Ludwig Maximilian University of Munich, INAF/IASF Milano, and European Space Agency - ESA
Subjects: PECULIAR VELOCITIES, PARTICLE HYDRODYNAMICS SIMULATIONS, individual: Messier 31 [galaxies], BULK FLOW, galaxies [submillimeter], Astrophysics::Cosmology and Extragalactic Astrophysics, WIENER RECONSTRUCTION, STAR-FORMATION RATES, Cosmic background radiation, COSMOLOGICAL IMPLICATIONS, SPHERE, LOCAL GROUP GALAXIES, PRE-LAUNCH STATUS, COSMIC WEB, SPINNING DUST EMISSION, ISM [submillimeter], observations [Cosmology], COMPLETE CO SURVEY, Astrophysics::Galaxy Astrophysics, SPITZER-SPACE-TELESCOPE, polarization, REDSHIFT SURVEYS, RESOLUTION IRAS MAPS, ISM [galaxies], general [ISM], WAVELETS, Astrophysics::Instrumentation and Methods for Astrophysics, GALAXY SAMPLES, FAR-INFRARED LUMINOSITY, RADIO-CONTINUUM SURVEY, galaxies [radio continuum], CLOUD, general [Planets and satellites], GALAXY, ROTATION MEASURES, clusters: intracluster medium [galaxies], MULTIBAND IMAGING PHOTOMETER, IRAS-GALAXIES, structure [galaxies], MORPHOLOGY, DARK ENERGY, large-scale structure of Universe, DENSITY FIELDS, Astrophysics::Earth and Planetary Astrophysics, EMISSION
Abstract: Measurements of flux density are described for five planets, Mars, Jupiter, Saturn, Uranus, and Neptune, across the six Planck High Frequency Instrument frequency bands (100-857 GHz) and these are then compared with models and existing data. In our analysis, we have also included estimates of the brightness of Jupiter and Saturn at the three frequencies of the Planck Low Frequency Instrument (30, 44, and 70 GHz). The results provide constraints on the intrinsic brightness and the brightness time-variability of these planets. The majority of the planet flux density estimates are limited by systematic errors, but still yield better than 1% measurements in many cases. Applying data from Planck HFI, the Wilkinson Microwave Anisotropy Probe (WMAP), and the Atacama Cosmology Telescope (ACT) to a model that incorporates contributions from Saturn's rings to the planet's total flux density suggests a best fit value for the spectral index of Saturn's ring system of βring = 2.30 ± 0.03 over the 30-1000 GHz frequency range. Estimates of the polarization amplitude of the planets have also been made in the four bands that have polarization-sensitive detectors (100-353 GHz); this analysis provides a 95% confidence level upper limit on Mars's polarization of 1.8, 1.7, 1.2, and 1.7% at 100, 143, 217, and 353 GHz, respectively. The average ratio between the Planck-HFI measurements and the adopted model predictions for all five planets (excluding Jupiter observations for 353 GHz) is 1.004, 1.002, 1.021, and 1.033 for 100, 143, 217, and 353 GHz, respectively. Model predictions for planet thermodynamic temperatures are therefore consistent with the absolute calibration of Planck-HFI detectors at about the three-percent level. We compare our measurements with published results from recent cosmic microwave background experiments. In particular, we observe that the flux densities measured by Planck HFI and WMAP agree to within 2%. These results allow experiments operating in the mm-wavelength range to cross-calibrate against Planck and improve models of radiative transport used in planetary science.
Published: 2017

46. Effect of KIT and PDGFRA mutations on survival in patients with gastrointestinal stromal tumors treated with adjuvant imatinib

Author: Joensuu, Heikki, Wardelmann, Eva, Sihto, Harri, Eriksson, Mikael, Sundby Hall, Kirsten, Reichardt, Annette, Hartmann, Jörg T., Pink, Daniel, Cameron, Silke, Hohenberger, Peter, Al-Batran, Salah-Eddin, Schlemmer, Marcus, Bauer, Sebastian, Nilsson, Bengt, Kallio, Raija, Junnila, Jouni, Vehtari, Aki, Reichardt, Peter, University of Helsinki, University Hospital Münster, Lund University, University of Oslo, HELIOS Klinikum Berlin-Buch, Franziskus Hospital, HELIOS Klinikum Wuppertal, University of Göttingen, Heidelberg University, Krankenhaus Nordwest, Ludwig Maximilian University of Munich, University of Duisburg-Essen, University of Gothenburg, University of Oulu, Pharma Ltd, Department of Computer Science, Aalto-yliopisto, and Aalto University
Abstract: IMPORTANCE: Little is known about whether the duration of adjuvant imatinib influences the prognostic significance of KIT proto-oncogene receptor tyrosine kinase (KIT) and platelet-derived growth factor receptor α (PDGFRA) mutations. OBJECTIVE: To investigate the effect of KIT and PDGFRA mutations on recurrence-free survival (RFS) in patients with gastrointestinal stromal tumors (GISTs) treated with surgery and adjuvant imatinib. DESIGN, SETTING, AND PARTICIPANTS: This exploratory study is based on the Scandinavian Sarcoma Group VIII/Arbeitsgemeinschaft Internistische Onkologie (SSGXVIII/AIO) multicenter clinical trial. Between February 4, 2004, and September 29, 2008, 400 patients who had undergone surgery for GISTs with a high risk of recurrence were randomized to receive adjuvant imatinib for 1 or 3 years. Of the 397 patients who provided consent, 341 (85.9%) had centrally confirmed, localized GISTs with mutation analysis for KIT and PDGFRA performed centrally using conventional sequencing. During a medianfollow-up of 88 months (completed December 31, 2013), 142 patients had GIST recurrence. Data of the evaluable population were analyzed February 4, 2004, through December 31, 2013. MAIN OUTCOMES AND MEASURES: The main outcome was RFS. Mutations were grouped by the gene and exon. KIT exon 11 mutations were further grouped as deletion or insertion-deletion mutations, substitution mutations, insertion or duplication mutations, and mutations that involved codons 557 and/or 558. RESULTS: Of the 341 patients (175 men and 166 women; median age at study entry, 62 years) in the 1-year group and 60 years in the 3-year group), 274 (80.4%) had GISTs with a KIT mutation, 43 (12.6%) had GISTs that harbored a PDGFRA mutation, and 24 (7.0%) had GISTs that were wild type for these genes. PDGFRA mutations and KIT exon 11 insertion or duplication mutations were associated with favorable RFS, whereas KIT exon 9 mutations were associated with unfavorable outcome. Patients with KIT exon 11 deletion or insertion-deletion mutation had better RFS when allocated to the 3-year group compared with the 1-year group (5-year RFS, 71.0% vs 41.3%; P < .001), whereas no significant benefit from the 3-year treatment was found in the other mutational subgroups examined. KIT exon 11 deletion mutations, deletions that involved codons 557 and/or 558, and deletions that led to pTrp557-Lys558del were associated with poor RFS in the 1-year group but not in the 3-year group. Similarly, in the subset with KIT exon 11 deletion mutations, higher-than-the-median mitotic counts were associated with unfavorable RFS in the 1-year group but not in the 3-year group. CONCLUSIONS AND RELEVANCE: Patients with KIT exon 11 deletion mutations benefit most from the longer duration of adjuvant imatinib. The duration of adjuvant imatinib modifies the risk of GIST recurrence associated with some KIT mutations, including deletions that affect exon 11 codons 557 and/or 558.
Published: 2017

47. Sentinel surveillance of imported dengue via travellers to Europe 2012 to 2014 : TropNet data from the DengueTools Research Initiative

Author: Francesco Castelli, Florian Kurth, Annelies Wilder-Smith, Elena Sulleiro, Andreas Neumayr, Francesca F. Norman, Antonia Calvo-Cano, Alberto Matteelli, Marjan Van Esbroeck, Andrea Angheben, Laurence Rochat, Jan Clerinx, Jakob P. Cramer, Guido Calleri, Rogelio López-Vélez, Leticia Franco, for TropNet, Blaise Genton, Núria Serre-Delcor, Jose Muñoz, Leo G. Visser, Federico Gobbi, David Poluda, María Paz Sánchez-Seco, Miguel J. Martínez, Emmanuel Bottieau, Ilaria Torta, Christoph Hatz, Thomas Zoller, Mirjam Schunk, Institut Català de la Salut, [Neumayr A] Department of Medicine and Diagnostics, Swiss Tropical and Public Health Institute, Basel, Switzerland. University of Basel, Switzerland. [Muñoz J] ISGlobal, Barcelona Centre for International Health Research (CRESIB), Hospital Clínic-Universitat de Barcelona, Barcelona, Spain. [Schunk M] Division of Infectious Diseases and Tropical Medicine, Medical Centre of the Ludwig-Maximilian-University (LMU), Munich, Germany. [Bottieau E] Department of Clinical Sciences, Institute of Tropical Medicine, Antwerp, Belgium. [Cramer J] Department of Internal Medicine I, Section Tropical Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany, Department of Clinical Research, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany. [Calleri G] Travel Medicine Unit, Department of Infectious Diseases, Amedeo di Savoia Hospital- ASLTO2, Torino, Italy. [Serre-Delcor N] Centre de Salut Internacional i Malalties Transmissibles Drassanes-Vall d'Hebron, Barcelona, Spain. Vall d’Hebron Hospital Universitari, Barcelona, Spain. PROSICS, Institut Català de la Salut, Barcelona, Spain. [Sulleiro E] Servei de Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. PROSICS Barcelona. Universitat Autònoma de Barcelona, Bellaterra, Spain, Vall d'Hebron Barcelona Hospital Campus, and for TropNet
Subjects: Epidemiology, Infektionsmedicin, Dengue virus, 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit, Investigative Techniques::Epidemiologic Methods::Investigative Techniques::Epidemiologic Methods::Sentinel Surveillance [ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT], medicine.disease_cause, Surveillance and Outbreak Report, Medical care, Disease Outbreaks, Dengue fever, Dengue, 0302 clinical medicine, 030212 general & internal medicine, técnicas de investigación::métodos epidemiológicos::técnicas de investigación::métodos epidemiológicos::vigilancia centinela [TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS], Asia, Southeastern, Phylogeny, Travel, Surveillance, Reverse Transcriptase Polymerase Chain Reaction, Incidence, Public Health, Global Health, Social Medicine and Epidemiology, Europe, importation, travel, travellers, Africa, Americas, Dengue Virus, Genotype, Humans, Travel Medicine, Sentinel Surveillance, Public Health, Environmental and Occupational Health, Virology, Importation, Geography, Otros calificadores::Otros calificadores::/transmisión [Otros calificadores], Public Health, Travellers, medicine.medical_specialty, Infectious Medicine, Asia, 030231 tropical medicine, Southeastern, Research initiative, 03 medical and health sciences, Dar es salaam, Environmental health, medicine, Africa/epidemiology, Americas/epidemiology, Asia, Southeastern/epidemiology, Dengue/diagnosis, Dengue/epidemiology, Dengue/transmission, Dengue Virus/genetics, Dengue Virus/isolation & purification, Europe/epidemiology, Reverse Transcriptase Polymerase Chain Reaction/methods, Travel Medicine/methods, Other subheadings::Other subheadings::/transmission [Other subheadings], Environmental and Occupational Health, Outbreak, medicine.disease, Dengue - Transmissió, Salut pública, Virus Diseases::Arbovirus Infections::Dengue [DISEASES], Folkhälsovetenskap, global hälsa, socialmedicin och epidemiologi, 2009 Bolivian dengue fever epidemic, virosis::infecciones por arbovirus::dengue [ENFERMEDADES]
Abstract: Dengue; Vigilància; Importació Dengue; Vigilancia; Importación Dengue; Surveillance; Importation We describe the epidemiological pattern and genetic characteristics of 242 acute dengue infections imported to Europe by returning travellers from 2012 to 2014. The overall geographical pattern of imported dengue (South-east Asia > Americas > western Pacific region > Africa) remained stable compared with 1999 to 2010. We isolated the majority of dengue virus genotypes and epidemic lineages causing outbreaks and epidemics in Asia, America and Africa during the study period. Travellers acted as sentinels for four unusual dengue outbreaks (Madeira, 2012–13; Luanda, 2013; Dar es Salaam, 2014; Tokyo, 2014). We were able to characterise dengue viruses imported from regions where currently no virological surveillance data are available. Up to 36% of travellers infected with dengue while travelling returned during the acute phase of the infection (up to 7 days after symptom onset) or became symptomatic after returning to Europe, and 58% of the patients with acute dengue infection were viraemic when seeking medical care. Epidemiological and virological data from dengue-infected international travellers can add an important layer to global surveillance efforts. A considerable number of dengue-infected travellers are viraemic after arrival back home, which poses a risk for dengue introduction and autochthonous transmission in European regions where suitable mosquito vectors are prevalent.
Published: 2017

48. Planck 2015 results

Author: Adam, R., Ade, P. A R, Aghanim, N., Akrami, Y., Alves, M. I R, Arguëso, F., Arnaud, M., Arroja, F., Ashdown, M., Aumont, J., Baccigalupi, C., Ballardini, M., Banday, A. J., Barreiro, R. B., Bartlett, J. G., Bartolo, N., Basak, S., Battaglia, P., Battaner, E., Battye, R., Benabed, K., Benoît, A., Benoit-Lévy, A., Bernard, J. P., Bersanelli, M., Bertincourt, B., Bielewicz, P., Bikmaev, I., Bock, J. J., Böhringer, H., Bonaldi, A., Bonavera, L., Bond, J. R., Borrill, J., Bouchet, F. R., Boulanger, F., Bucher, M., Burenin, R., Burigana, C., Butler, R. C., Calabrese, E., Cardoso, J. F., Carvalho, P., Casaponsa, B., Castex, G., Catalano, A., Challinor, A., Chamballu, A., Chary, R. R., Chiang, H. C., Chluba, J., Chon, G., Christensen, P. R., Church, S., Clemens, M., Clements, D. L., Colombi, S., Colombo, L. P L, Combet, C., Comis, B., Contreras, D., Couchot, F., Coulais, A., Crill, B. P., Cruz, M., Curto, A., Cuttaia, F., Danese, L., Davies, R. D., Davis, R. J., De Bernardis, P., De Rosa, A., De Zotti, G., Delabrouille, J., Delouis, J.-M., Désert, F. X., Di Valentino, E., Dickinson, C., Diego, J. M., Dolag, K., Dole, H., Donzelli, S., Doré, O., Douspis, M., Ducout, A., Dunkley, J., Dupac, X., Efstathiou, G., Eisenhardt, P. R M, Elsner, F., Enßlin, T. A., Eriksen, H. K., Falgarone, E., Fantaye, Y., Farhang, M., Feeney, S., Fergusson, J., Fernandez-Cobos, R., Feroz, F., Finelli, F., Florido, E., Forni, O., Frailis, M., Fraisse, A. A., Franceschet, C., Franceschi, E., Frejsel, A., Frolov, A., Galeotta, S., Galli, S., Ganga, K., Gauthier, C., Génova-Santos, R. T., Gerbino, M., Ghosh, T., Giard, M., Giraud-Héraud, Y., Giusarma, E., Gjerløw, E., González-Nuevo, J., Górski, K. M., Grainge, K. J B, Gratton, S., Gregorio, A., Gruppuso, A., Gudmundsson, J. E., Hamann, J., Handley, W., Hansen, F. K., Hanson, D., Harrison, D. L., Heavens, A., Helou, G., Henrot-Versillé, S., Hernández-Monteagudo, C., Herranz, D., Hildebrandt, S. R., Hivon, E., Hobson, M., Holmes, W. A., Hornstrup, A., Hovest, W., Huang, Z., Huffenberger, K. M., Hurier, G., Ilić, S., Jaffe, A. H., Jaffe, T. R., Jin, T., Jones, W. C., Juvela, M., Karakci, A., Keihänen, E., Keskitalo, R., Khamitov, I., Kiiveri, K., Kim, Jaiseung, Kisner, T. S., Kneissl, R., Knoche, J., Knox, L., Krachmalnicoff, N., Kunz, M., Kurki-Suonio, H., Lacasa, F., Lagache, G., Lähteenmäki, A., Lamarre, J. M., Langer, M., Lasenby, A., Lattanzi, M., Lawrence, C. R., Le Jeune, M., Leahy, J. P., Lellouch, E., Leonardi, R., León-Tavares, J., Lesgourgues, J., Levrier, F., Lewis, A., Liguori, M., Lilje, P. B., Lilley, M., Linden-Vørnle, M., Lindholm, V., Liu, H., López-Caniego, M., Lubin, P. M., Ma, Y.-Z., Maciás-Pérez, J. F., Maggio, G., Maino, D., Mak, D. S Y, Mandolesi, N., Mangilli, A., Marchini, A., Marcos-Caballero, A., Marinucci, D., Maris, M., Marshall, D. J., Martin, P. G., Martinelli, M., Martínez-González, E., Masi, S., Matarrese, S., Mazzotta, P., Mcewen, J. D., Mcgehee, P., Mei, S., Meinhold, P. R., Melchiorri, A., Melin, J. B., Mendes, L., Mennella, A., Migliaccio, M., Mikkelsen, K., Millea, M., Mitra, S., Miville-Deschênes, M. A., Molinari, D., Moneti, A., Montier, L., Moreno, R., Morgante, G., Mortlock, D., Moss, A., Mottet, S., Münchmeyer, M., Munshi, D., Murphy, J. A., Narimani, A., Naselsky, P., Nastasi, A., Nati, F., Natoli, P., Negrello, M., Netterfield, C. B., Nørgaard-Nielsen, H. U., Noviello, F., Novikov, D., Novikov, I., Olamaie, M., Oppermann, N., Orlando, E., Oxborrow, C. A., Paci, F., Pagano, L., Pajot, F., Paladini, R., Pandolfi, S., Paoletti, D., Partridge, B., Pasian, F., Patanchon, G., Pearson, T. J., Peel, M., Peiris, H. V., Pelkonen, V. M., Perdereau, O., Perotto, L., Perrott, Y. C., Perrotta, F., Pettorino, V., Piacentini, F., Piat, M., Pierpaoli, E., Pietrobon, D., Plaszczynski, S., Pogosyan, D., Pointecouteau, E., Polenta, G., Popa, L., Pratt, G. W., Prézeau, G., Prunet, S., Puget, J. L., Rachen, J. P., Racine, B., Reach, W. T., Rebolo, R., Reinecke, M., Remazeilles, M., Renault, C., Renzi, A., Ristorcelli, I., Rocha, G., Roman, M., Romelli, E., Rosset, C., Rossetti, M., Rotti, A., Roudier, G., Rouillé D'orfeuil, B., Rowan-Robinson, M., Rubinõ-Martín, J. A., Ruiz-Granados, B., Rumsey, C., Rusholme, B., Said, N., Salvatelli, V., Salvati, L., Sandri, M., Sanghera, H. S., Santos, D., Saunders, R. D E, Sauvé, A., Savelainen, M., Savini, G., Schaefer, B. M., Schammel, M. P., Scott, D., Seiffert, M. D., Serra, P., Shellard, E. P S, Shimwell, T. W., Shiraishi, M., Smith, K., Souradeep, T., Spencer, L. D., Spinelli, M., Stanford, S. A., Stern, D., Stolyarov, V., Stompor, R., Strong, A. W., Sudiwala, R., Sunyaev, R., Sutter, P., Sutton, D., Suur-Uski, A. S., Sygnet, J. F., Tauber, J. A., Tavagnacco, D., Terenzi, L., Texier, D., Toffolatti, L., Tomasi, M., Tornikoski, M., Tramonte, D., Tristram, M., Troja, A., Trombetti, T., Tucci, M., Tuovinen, J., Türler, M., Umana, G., Valenziano, L., Väliviita, J., Van Tent, F., Vassallo, T., Vibert, L., Vidal, M., Viel, M., Vielva, P., Villa, F., Wade, L. A., Walter, B., Wandelt, B. D., Watson, R., Wehus, I. K., Welikala, N., Weller, J., White, M., White, S. D M, Wilkinson, A., Yvon, D., Zacchei, A., Zibin, J. P., Zonca, A., Cardiff University, Institut national de physique nucléaire et de physique des particules, Kavli Institute for Cosmology Cambridge, International School for Advanced Studies, IRAP, Universidad de Cantabria, Sapienza University of Rome, Università Degli Studi di Trieste, Instituto Carlos I de Física Teórica y Computacional, UMR7095, INAF/IASF Milano, Jet Propulsion Laboratory, University of Manchester, University of Toronto, University of California Berkeley, Institut d 'Astrophysique de Paris, Université Sorbonne Paris Cité, Istituto di Astrofisica Spaziale e Fisica Cosmica di Bologna, University of Oxford, Telecom ParisTech, Service d'Astrophysique CEA, Niels Bohr Institute, Jodrell Bank Centre for Astrophysics, Università La Sapienza, Centre National de la Recherche Scientifique (CNRS), Urbanización Villafranca Del Castillo, University of Cambridge, Max-Planck-Institut für Astrophysik, University of Oslo, Osservatorio Astronomico di Trieste, University of Chicago, University of Warsaw, McGill University, Université Paris-Sud, Danmarks Tekniske Universitet, Florida State University, Imperial College London, University of Helsinki, Lawrence Berkeley National Laboratory, University of Milano, Department of Radio Science and Engineering, LERMA - Laboratoire d'Etudes du Rayonnement et de la Matiere en Astrophysique et Atmospheres, University of Ferrara, Centro de Gestão e Estudos Estratégicos, CERN, University of California Santa Barbara, California Institute of Technology, University of Nottingham, National University of Ireland, Galway, Princeton University, RAS - P.N. Lebedev Physics Institute, Haverford College, Institut für Theoretische Astrophysik, University of Southern California, Osservatorio Astronomico Roma, Instituto de Astrofísica de Canarias, Università di Roma Tor Vergata, University of British Columbia, Special Astrophysical Observatory of the Russian Academy of Sciences, European Space Research and Technology Centre, Università degli Studi eCampus, University of Geneva, Trinity College Dublin, INAF, Osservatorio Astrofisico di Catania, Université Paris Diderot, Aalto-yliopisto, Aalto University, Stanford University, Stockholm University, University of Sussex, Institute for Space Sciences, Universities Space Research Association, University College London, Space Research Institute of the Russian Academy of Sciences, CNRS/IN2P3, Universite de Toulouse, Instituto de Física de Cantabria (CSIC-Universidad de Cantabria), Jet Propulsion Laboratory, California Institute of Technology, AstroParticule et Cosmologie, Università Degli Studi di Padova, Istituto Nazionale di Fisica Nucleare, Sezione di Padova, I-35131 Padova, Italy, University of Granada, CNRS, Università degli Studi di Milano, Nicolaus Copernicus Astronomical Center, University of California at Berkeley, Universite Paris Sorbonne - Paris IV, INAF/IASF Bologna, Università di Ferrara, INFN, Sezione di Bologna, UMR 5141, Laboratoire AIM, Service d’Astrophysique, DSM\IRFU, CEA\Saclay, Institut d'Astrophysique Spatiale, University of KwaZulu-Natal, Johns Hopkins University, INAF, Osservatorio Astronomico di Padova, UMR 7095, Ludwig Maximilian University of Munich, Institut Universitaire de France, European Space Agcy, European Space Agency, ESAC, Planck Sci Off, Shahid Beheshti University, National Taiwan University, Stockholms universitet, NORDITA, Istituto Nazionale di Fisica Nucleare, University of Sydney, Centro de Estudios de la Física del Cosmos de Aragón, Technical University of Denmark, European Southern Observatory Santiago, ALMA Santiago Central Offices, University of California, Université de Genève, African Institute for Mathematical Sciences, Helsinki Institute of Physics, Aix Marseille Universite, Metsähovi Radio Observatory, INFN, Sezione di Ferrara, RWTH Aachen University, INFN, Sezione di Padova, University of California, Santa Barbara, INAF, Osservatorio Astronomico di Trieste, Universite Paris-Sud, INFN, Sezione di Roma 1, University of Heidelberg, Gran Sasso Science Institute, CEA Saclay, CEA, DSM Irfu SPP, Inter-University Centre for Astronomy and Astrophysics, CNRS Centre National de la Recherche Scientifique, National University of Ireland, University of Copenhagen, ASI Science Data Center, RAS - Pn Lebedev Physics Institute, INAF, Osservatorio Astronomico di Roma, Université Pierre and Marie Curie, Radboud University Nijmegen, Instituto Astrofisico de Canarias, CSIC, Universidad de La Laguna, Department of Applied Physics, ROTA – Topological superfluids, Special Astrophysical Observatory, Russian Academy of Sciences, Kazan Federal University, Space Research Institute, Russian Academy of Sciences, ESTEC - European Space Research and Technology Centre, Università degli Studi e-Campus, Universidad de Oviedo, University of Illinois at Urbana-Champaign, Université Paris-Saclay, University of Oviedo, IRFM-CEA, CEA Saclay, University of Bologna, Astroparticle and Cosmology Laboratory, University of Padova, Max Planck Institute for Extraterrestrial Physics, Russian Academy of Sciences, INAF - Osservatorio Astronomico di Padova, Institut de Planétologie et d'Astrophysique de Grenoble, European Space Astronomy Centre, INAF - Osservatorio Astronomico di Trieste, Simon Fraser University, TÛBITAK National Observatory, University of California Davis, Heidelberg University, Observatoire de Paris, University of Alberta, Inter-University Centre for Astronomy and Astrophysics India, Leiden University, Perimeter Institute for Theoretical Physics, Ludwig-Maximilians-University, University Observatory Munich, Facultad de Ciencias, Université Paris 13, School of Physics and Astronomy, Osservatorio Astronomicodi Roma, Universite Joseph Fourier, Université Grenoble Alpes, and European Space Agency - ESA
Subjects: statistical [Methods], Diffuse radiation, Large-scale structure of Universe, Astrophysics::High Energy Astrophysical Phenomena, Local insterstellar matter, Cosmological parameters, Astrophysics::Cosmology and Extragalactic Astrophysics, Surveys, clusters: general [Galaxies], Cosmic background radiation, Early Universe, instruments [Space vehicles], theory [Cosmology], Polarization, Dark energy, observations [Cosmology], data analysis [Methods], general [Galaxy], Astrophysics::Galaxy Astrophysics, detectors [Instrumentation], general [Radiation mechanisms], polarimeters [Instrumentation], general [ISM], Astrophysics::Instrumentation and Methods for Astrophysics, Astronomy and Astrophysics, Inflation, ISM [Radio continuum], Space and Planetary Science, 115 Astronomy and space science, Magnetic fields, Gravitation
Abstract: This paper presents cosmological results based on full-mission Planck observations of temperature and polarization anisotropies of the cosmic microwave background (CMB) radiation. Our results are in very good agreement with the 2013 analysis of the Planck nominal-mission temperature data, but with increased precision. The temperature and polarization power spectra are consistent with the standard spatially-flat 6-parameter ΛCDM cosmology with a power-law spectrum of adiabatic scalar perturbations (denoted "base ΛCDM" in this paper). From the Planck temperature data combined with Planck lensing, for this cosmology we find a Hubble constant, H0 = (67.8 ± 0.9) km s-1Mpc-1, a matter density parameter Ωm = 0.308 ± 0.012, and a tilted scalar spectral index with ns = 0.968 ± 0.006, consistent with the 2013 analysis. Note that in this abstract we quote 68% confidence limits on measured parameters and 95% upper limits on other parameters. We present the first results of polarization measurements with the Low FrequencyInstrument at large angular scales. Combined with the Planck temperature and lensing data, these measurements give a reionization optical depth of τ = 0.066 ± 0.016, corresponding to a reionization redshift of \hbox{$z-{\rm re}=8.8{+1.7}-{-1.4}$}. These results are consistent with those from WMAP polarization measurements cleaned for dust emission using 353-GHz polarization maps from the High Frequency Instrument. We find no evidence for any departure from base ΛCDM in the neutrino sector of the theory; for example, combining Planck observations with other astrophysical data we find Neff = 3.15 ± 0.23 for the effective number of relativistic degrees of freedom, consistent with the value Neff = 3.046 of the Standard Model of particle physics. The sum of neutrino masses is constrained to â'mν < 0.23 eV. The spatial curvature of our Universe is found to be very close to zero, with | ΩK | < 0.005. Adding a tensor component as a single-parameter extension to base ΛCDM we find an upper limit on the tensor-to-scalar ratio of r0.002< 0.11, consistent with the Planck 2013 results and consistent with the B-mode polarization constraints from a joint analysis of BICEP2, Keck Array, and Planck (BKP) data. Adding the BKP B-mode data to our analysis leads to a tighter constraint of r0.002 < 0.09 and disfavours inflationarymodels with a V(φ) φ2 potential. The addition of Planck polarization data leads to strong constraints on deviations from a purely adiabatic spectrum of fluctuations. We find no evidence for any contribution from isocurvature perturbations or from cosmic defects. Combining Planck data with other astrophysical data, including Type Ia supernovae, the equation of state of dark energy is constrained to w =-1.006 ± 0.045, consistent with the expected value for a cosmological constant. The standard big bang nucleosynthesis predictions for the helium and deuterium abundances for the best-fit Planck base ΛCDM cosmology are in excellent agreement with observations. We also constraints on annihilating dark matter and onpossible deviations from the standard recombination history. In neither case do we find no evidence for new physics. The Planck results for base ΛCDM are in good agreement with baryon acoustic oscillation data and with the JLA sample of Type Ia supernovae. However, as in the 2013 analysis, the amplitude of the fluctuation spectrum is found to be higher than inferred from some analyses of rich cluster counts and weak gravitational lensing. We show that these tensions cannot easily be resolved with simple modifications of the base ΛCDM cosmology. Apart from these tensions, the base ΛCDM cosmology provides an excellent description of the Planck CMB observations and many other astrophysical data sets.
Published: 2016

49. Reproducibility of Fluorescent Expression from Engineered Biological Constructs in E. coli

Author: Jacob, Beal, Trac Haddock Angellii, Markus, Gershater, Kim de Mora, Meagan, Lizarazo, Jim, Hollenhorst, Randy, Rettberg, Philipp, Demling, Rene, Hanke, Michae, Osthegel, Anna, Schechtel, Suresh, Sudarsan, Arne, Zimmermann, Bartosz, Gabryelczyk, Martina, Ikonen, Minnamari, Salmela, Muradıye, Acar, Muhammed Fatih Aktas, Furkan, Bestepe, Furkan Sacit Ceylan, Sadık, Cigdem, Mikail, Dohan, Mustafa, Elitok, Mehmet, Gunduz, Esra, Gunduz, Omer Faruk Hatipoglu, Turan, Kaya, Orhan, Sayin, Safa, Tapan, Osman Faruk Tereci, Abdullah, Uçar, Mustafa, Yilmaz, Jeffrey, Barrick, Alex, Gutierrez, Dennis, Mishler, Jordan, Monk, Kate, Mortensen, Nathan, Shin, Ella, Watkins, Yintong, Chen, Yuji, Jin, Yuanjie, Shi, Haoqian Myelin Zhang, Bruno, Ono, Ieda Maria Martinez Paino, Lais, Ribovski, Ivan, Silva, Danilo Keiji Zampronio, Nils, Birkholz, Rudiger Frederik Busche, Oliver, Konzock, Steffen, Lippold, Carsten, Ludwig, Melanie, Philippi, Lukas, Platz, Christian, Sigismund, Susanne, Weber, Maren, Wehrs, Niels, Werchau, Anna, Wronska, Zen Zen Yen, Yash, Agarwal, Evan, Appleton, Douglas, Densmore, Ariela, Esmurria, Kathleen, Lewis, Alan, Pacheco, Marcel, Bruchez, Danielle, Peters, Cheryl, Telmer, Lena, Wang, Silvia Canas Duarte, Daniel Giraldo Perez, Camilo Gomez Garzon, Jorge Madrid Wolff, Nathaly Marin Medina, Valentina, Mazzanti, Laura Rodriguez Forero, Eitan, Scher, Robin, Dowell, Samantha, O’Hara, Cloe Simone Pogoda, Kendra, Shattuck, Ali, Altintas, Anne Pihl Bali, Rasmus, Bech, Anne, Egholm, Anne Sofie Laerke Hansen, Kristian, Jensen, Kristian Barreth Karlsen, Caroline, Mosbech, Sophia, Belkhelfa, Noemie, Berenger, Romain, Bodinier, Cecile, Jacry, Laura Matabishi Bibi, Pierre, Parutto, Julie, Zaworski, Andries de Vries, Freek de Wijs, Rick, Elbert, Lisa, Hielkema, Chandhuru, Jagadeesan, Bayu, Jayawardhana, Oscar, Kuipers, Anna, Lauxen, Thomas, Meijer, Sandra, Mous, Renske van Raaphorst, Aakanksha, Saraf, Otto, Schepers, Oscar, Smits, Jan Willem Veening, Ruud Detert Oude Weme, Lianne, Wieske, Catherine, Ainsworth, Xenia Spencer Milnes, Alejandro, Gómezávila, Eddie Cano Gamez, Ana Laura Torres Huerta, Carlos Alejandro Meza Ramirez, Philipp, Popp, Jara, Radeck, Anna, Sommer, Xiangkai, Li, Qi, Wu, Hongxia, Zhao, Ruixue, Zhao, Irem, Bastuzel, Yasemin, Ceyhan, Mayda, Gursel, Burak, Kizil, Ilkem, Kumru, Yasemin, Kuvvet, Helin, Tercan, Seniz, Yuksel, Luiza, Niyazmetova, Timothy, Ang, Lucas, Black, Ciaran, Kelly, George, Wadhams, Clovis, Basier, Urszula, Czerwinska, Cindy Suci Ananda, Muhammad Al Azhar, Adelia, Elviantari, Maya, Fitriana, Arief Budi Witarto, Yuliant, Jia Fangxing, Qingfeng, Hou, Wan, Pei, Chen, Rifei, Wang, Rong, Huang, Wei, Zhang, Yushan, Jianguo, He, Dengwen, Lai, Pai, Li, Jianheng, Liu, Chunyang, Ni, Qianbin, Zhang, Cinthya, Cadenas, Zardain Canabal, Eduardo J., Claudia Nallely Alonso Cantu, Mercedes Alejandra Vazquez Cantu, Eduardo Cepeda Canedo, Cesar Miguel Valdez Cordova, Jose Alberto de la Paz Espinosa, Carlos Enrique Alavez Garcia, Ana Laura Navarro Heredia, Adriana, Hernandez, Sebastian Valdivieso Jauregui, Eduardo Ramirez Montiel, Eduardo Serna Morales, Yamile Minerva Castellanos Morales, Omar Alonso Cantu Pena, Ramirez Rodríguez, Eduardo A., Elizabeth Vallejo Trejo, Jesus Gilberto Rodriguez Ceja, Jesus Eduardo Martinez Hernandez, Mario Alberto Pena Hernandez, Enrique Amaya Perez, Rebeca Paola Torres Ramirez, Cla, J., Martin, Hanzel, Sarah Mohand Said, Shihab, Sawar, Dylan, Siriwardena, Alex, Tzahristos, Nils, Anlind, Martin, Friberg, Erik, Gullberg, Stephanie, Herman, Dallin, Christensen, Sara, Gertsch, Cody, Maxfield, Charles, Miller, Ryan, Putman, Christine, Bauerl, Estelles Lopez, Lucia T., Estefania Huet Trujillo, Marta Vazquez Vilar, Marlène Sophie Birk, Nico, Claassens, Walter de Koster, Rik van Rosmalen, Wen Ying Wu, Sian, Davies, Dan, Goss, William, Rostain, Chelsey, Tye, Waqar, Yousaf, Natalie, Farny, Chloe, Lajeunesse, Alex, Turland, Chen, An, Jielin, Chen, Yahong, Chen, Zehua, Che, Baishan, Fang, Xiaotong, Fu, Xifeng, Guo, Yue, Jiang, Yiying, Lei, Jianqiao, Li, Zhe, Li, Chang, Liu, Weibing, Liu, Yang, Li, Yizhu, Lv, Qingyu, Ruan, Yue, Su, Chun, Tang, Yushen, Wang, Fan, Wu, Xiaoshan, Yan, Ruihua, Zhang, Tangduo, Zhang, Farren, Isaacs, Ariel Leyva Hernandez, Natalie, Ma, Stephanie, Mao, Yamini, Naidu, Tuukka, Miinalainen, Marion Aruann, Daniel Calendini, Yoann Chabert, Gael Chambonnier, Myriam, Choukour, Ella de Gaulejac, Camille, Houy, Axel, Levier, Loreen, Logger, Sebastien, Nin, Valerie, Prima, Sturgis, James N., Beibei, Fang, Sadik, Cigdem, Abdullah, Ucar, Alejandro, Gutierrez, Revanth, Poondla, Sanjana, Reddy, Tyler, Rocha, Natalie, Schulte, Devin, Wehle, Marta Eva Jackowski, Sean Ross Craig, Ariana Mirzarafie Ahi, Elliott, Parris, Luba, Prout, Barbara, Steijl, Rachel, Wellman, Zhao, Fan, Zhang, Jing, Yang, Wei, Yang, Yuanzhan, Wen, Zhaosen, Evan, Appletion, Jeffrey, Chen, Abha, Patil, Shaheer, Priracha, Kate, Ryan, Nick, Salvador, John, Viola, Boralli, Camila Maria S., Camila Barbosa Bramorski, Juliana Cancino Bernardi, Ana Laura de Lima, Paula Maria Pincela Lins, Cristiane Casonato Melo, Deborah Cezar Mendonca, Thiago, Mosqueiro, Everton, Silva, Graziele, Vasconcelos, Ruchi, Asthana, Donna, Lee, Michelle, Yu, Peter, Choi, Effie, Lau, Kenneth, Lau, Oscar, Ying, Brandon, Malone, Paul, Young, Aidan, Ceney, Dakota, Hawthorne, Sharon, Lian, Sam, Mellentine, Dylan, Miller, Barbara Castro Moreira, Christie, Peebles, Olivia, Smith, Kevin, Walsh, Allison, Zimont, Michael, Brasino, Michael, Donovan, Hannah, Young, Jan, Bejvl, Daniel, Georgiev, Hynek, Kasl, Katerina Pechotova, Vaclav Pelisek, Anna, Sosnova, Pavel, Zach, Anthony, Ciesla, Benjamin, Hoover, Elliott, Chapman, Jon Marles Wright, Vicky, Moynihan, Liusaidh, Owen, Brooke Rothschild Mancinelli, Emilie, Cuillery, Joseph, Heng, Vincent, Jacquot, Paola, Malsot, Rocco, Meli, Cyril, Pulver, Ari, Sarfatis, Loic, Steiner, Victor, Steininger, Nina van Tiel, Gregoire, Thouvenin, Axel, Uran, Lisa, Baumgartner, Anna, Fomitcheva, Daniel, Gerngross, Verena, Jagger, Michael, Meier, Anja, Michel, Jasmine, Bird, Bradley, Brown, Todd, Burlington, Daniel, Herring, Joseph, Slack, Georgina, Westwood, Emilia, Wojcik, Julian, Bender, Julia, Donauer, Ramona, Emig, Rabea, Jesser, Julika, Neumann, Lara, Stuhn, Takema, Hasegawa, Tomoya, Kozakai, Haruka, Maruyama, Sean, Colloms, Charlotte, Flynn, Vilija, Lomeikaite, James, Provan, Kang, Ning, Shuyan, Tang, Guozhao, Wu, Yunjun, Yang, Zhi, Zeng, Zhan, Yi, Pan, Chu, Jun, Li, Keji, Yan, Athale, Chaitanya A., Swapnil, Bodkhe, Manasi, Gangan, Harsh, Gakhare, Yash, Jawale, Snehal, Kadam, Prachiti, Moghe, Gayatri, Mundhe, Neha, Khetan, Ira, Phadke, Prashant, Uniyal, Siddhesh, Zadey, Ines, Cottignie, Eline, Deprez, Astrid, Deryckere, Jasper, Janssens, Frederik, Jonnaert, Katarzyna, Malczewska, Thomas, Pak, Johan, Robben, Ovia Margaret Thirukkumaran, Vincent Van Deuren, Laurens, Vandebroek, Laura Van Hese, Laetitia Van Wonterghem, Leen, Verschooten, Moritz, Wolter, Joss, Auty, Richard, Badge, Liam, Crawford, Raymond, Dalgleish, Amy, Evans, Cameron, Grundy, Charlie, Kruczko, Payal, Karia, Graeme, Glaister, Rhys, Hakstol, Seme, Mate, Karin, Otero, Dustin, Smith, Jeff, Tingley, Hans Joachim Wieden, Haotian, Wang, Ningning, Yao, Matthias, Franz, Anna, Knoerlein, Nicolas, Koutsoubelis, Loechner, Anne C., Max, Mundt, Alexandra, Richter, Oliver, Schauer, Marjorie, Buss, Sivateja, Tangirala, Brian, Teague, Tianyi, Huang, Xinhao, Song, Yibing, Wei, Zhaoran, Zhang, Longzhi, Cao, Cheng Li, Kang Yang, Zhiqin, Chen, Yuxing, Fang, Libo, Sun, Weiyi, Wang, Yang, Yang, David, Adams, Joshua, Colls, Joshua, Timmons, David, Urick, Julia Anna Adrian, Madina, Akan, Youssef, Chahibi, Rahmi, Lale, Typhaine Le Doujet, Marit Vaagen Roee, Altynay, Abdirakhmanova, Askarbek, Orakov, Azhar, Zhailauova, Jinyang, Liang, Yu, Ma, Qikai, Qin, Yetian, Su, Ju Yeon Han, Raphaella, Hull, Wei Chung Kong, Li Chieh Lu, Duke, Quinton, Pauline, Aubert, Johan, Bourdarias, Olivier, Bugaud, Coralie Demon Chaine, Isabelle, Hatin, Ibtissam Kaid Slimane, Seong Koo Kang, Audrey, Moatti, Cheikh Fall Ndiaye, Mathilde, Ananos, Alexander, Arkhipenko, Valentin, Bailly, Jules, Caput, Javier, Castillo, Alma Chapet Batlle, Floriane, Cherrier, Claudia Demarta Gatsi, Deshmukh, Gopaul, Muriel, Gugger, Caroline, Lambert, Lucas, Krauss, Amelie, Vandendaele, Xiaojing, Li, Lin, Xiaomei, Luo Xunxun, Anders C. h. r. Hansen, Tina, Kronborg, Pettersen, Jens S., Charles, Calvet, Tyler Dae Devlin, Kosuke, Fujishima, Danny, Greenberg, Tina, Ju, Ryan, Kent, Daniel, Kunin, Erica, Lieberman, Griffin, Mccutcheon, Thai, Nguyen, Lynn, Rothschild, Shih, Joseph D., Jack, Takahashi, Kirsten, Thompson, Forrest, Tran, Daniel, Xiang, Felix, Richter, Yang, Xiaoran, Xiangyue, Hu, Changyuan, Deng, Shuyu, Hua, Yumeng, Li, Xinyu, Meng, Boxiang, Wang, Yingqi, Wang, Xuan, Wang, Zixuan, Xu, Jieyu, Yan, Ming, Yan, Yineng, Zhou, Edgar Alberto Alcalá Orozco, José Alberto Cristerna Bermúdez, Daniela Flores Gómez, José Ernesto Hernández Castañeda, Diana Clarisse Montaño Navarro, Juana Yessica PérezÁvila, María Fernanda Salazar Figueroa, María Fernanda Sánchez Arroyo, Oliva Angélica Sánchez Montesinos, Ángel Farid Rojas Cruz, Carlos Ramos Gutiérrez, Alonso Pérez Lona, Carlos Alejandro Meza Ramírez, Fernanda Sotomayor Olivares, Jorge Sebastián Rodríguez Iniesta, Juan Carlos Rueda Silva, Shotaro, Ayukawa, Takahiro, Kashiwagi, Daisuke, Kiga, Misa, Minegishi, Riku, Shinohara, Hiraku, Tokuma, Yuta, Yamazaki, Shuhei, Yasunishi, Erinn Sim Zixuan, Remsha, Afzal, Matthew, Carrigan, Barry, Moran, Marlena, Mucha, Arnas, Petrauskas, Stefan, Marsden, Michelle, Post, Anne, Rodenburg, Hector, Sanguesa, Marit van der Does, Erwin van Rijn, Max van’t Hof, Yeshi de Bruin, Hans de Ferrante, Elles, Elschot, Laura, Jacobs, Jan Willem Muller, Sjoerd, Nooijens, Femke, Vaassen, Cas van der Putten, Esther van Leeuwen, Laura van Smeden, Kwan Kwan Zhu, Kevin, Sabath, Katharina, Sporbeck, Nicolai von Kügelgen, Lisa, Wellinger, Stefanie, Braun, Jack, Ho, Yash, Mishra, Mariola, Sebastian, Lucas von Chamier, Ahsan, Fasih M., Satyadi, Megan A., Vivienne, Gunadhi, Phillip, Kyriakakis, Jenny, Lee, Walter, Thavarajah, Kimia, Abtahi, Robert, Hand, Chun Mun Loke, Adam, Wahab, Iowis, Zhu, Del Bianco, Cristina, Chizzolini, Fabio, Elisa, Godino, Lentini, Roberta, Mansy, Sheref Samir, Yeh Martin, Noel, Claudio Oss Pegorar, Alexander, Cook, Timothy, Kerns, Chad, Nielsen, Michael, Paskett, Alexander, Torgesen, Stephen, Lee, Ophir, Ospovat, Sikandar, Raza, Daniel, Shaykevich, Jarrod, Shilts, Barbora, Bajorinaite, Mykolas, Bendorius, Ieva, Rauluseviciute, Ieva, Savickyte, Sarunas, Tumas, William, Buchser, Elli, Cryan, Caroline, Golino, Andrew, Halleran, Taylor, Jacobs, Michael, Lefew, Joe, Maniaci, John, Marken, Margaret, Saha, Panya, Vij, Kayla, Desanty, Julie, Mazza, Raytheon BBN Technologies, iGEM Foundation, Synthace, Agilent Technology [Santa Clara], Raytheon BBN Technologies, Synthace, and Agilent provided support in the form of salaries for authors JB, MG, and JH, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the author contributions section, The authors wish to thank Sarah Munro and Marc Salit of NIST for help in designing this study. Consortium authors include all persons self-identified by contributing teams as deserving co-authorship credit. Contributors are listed alphabetically within team, and teams alphabetically and by year. Note that some persons may be credited as contributing in both years. Team names are given as identified in iGEM records: full details of each team’s institution and additional members may be found online in the iGEM Foundation archives at:http://year.igem.org/Team:name e.g.: full information on the 2015 ETH_Zurich team may be found at: http://2015.igem.org/Team:ETH_Zurich, BBN Technologies, IGEM Foundation, Synthace Ltd., Agilent Technologies, Department of Biotechnology and Chemical Technology, Helsinki Institute for Information Technology HIIT, ATOMS Turkiye, Boston University, Carnegie Mellon University, Technical University of Denmark, Ludwig Maximilian University of Munich, Middle East Technical University, Sumbawa University of Technology, Southern University of Science and Technology, Sun Yat-Sen University, Tecnológico de Monterrey, Universidad Autonoma de Nuevo Leon, University of Ottawa, Universitat Politècnica de València, Wageningen University and Research Centre, Worcester Polytechnic Institute, Xiamen University, University of Texas at Austin, Bielefeld University, Birkbeck University of London, USP-Brazil, City University of Hong Kong, Colorado State University, CU Boulder, Swiss Federal Institute of Technology Lausanne, Swiss Federal Institute of Technology Zurich, University of Exeter, Indian Institute of Science Education and Research, KU Leuven, Massachusetts Institute of Technology, Northeast Agricultural University, Nanjing Agricultural University, Norwegian University of Science and Technology, Ocean University of China, University of Southern Denmark, Shenzhen Middle School - SZMS 15, Tokyo Institute of Technology, Trinity College Dublin, Delft University of Technology, Eindhoven University of Technology, Tuebingen, University of California Los Angeles, University of California San Diego, University of Maryland, University of Trento, Vanderbilt University, College of William and Mary, Department of Bioproducts and Biosystems, Aalto-yliopisto, Aalto University, Jones, D Dafydd, Discrete Technology and Production Automation, and Robotics and image-guided minimally-invasive surgery (ROBOTICS)
Subjects: 0106 biological sciences, 0301 basic medicine, green fluorescent protein, Laboratory Proficiency Testing, Transcription, Genetic, International Genetically Engineered Machine, [SDV]Life Sciences [q-bio], lcsh:Medicine, Protein Engineering, 01 natural sciences, Infographics, Synthetic biology, genetics, lcsh:Science, Promoter Regions, Genetic, Macromolecular Engineering, transcription initiation, Measurement, Multidisciplinary, Chemistry, Strain (biology), gene expression regulation, good laboratory practice, Research Assessment, Fluorescence, Reproducibility, 3. Good health, Bioassays and Physiological Analysis, Engineering and Technology, Educational Status, Synthetic Biology, Genetic Engineering, Transcription, Graphs, Research Article, Biotechnology, Transcriptional Activation, Computer and Information Sciences, General Science & Technology, Green Fluorescent Proteins, Bioengineering, Computational biology, iGEM Interlab Study Contributors, Research and Analysis Methods, Promoter Regions, 03 medical and health sciences, promoter region, Genetic, 010608 biotechnology, Escherichia coli, ta215, business.industry, Data Visualization, lcsh:R, Fluorescence Competition, genetic transcription, DNA structure, Reproducibility of Results, Biology and Life Sciences, protein engineering, 030104 developmental biology, Good Health and Well Being, 7 INGENIERÍA Y TECNOLOGÍA, Synthetic Bioengineering, People and Places, lcsh:Q, Population Groupings, biosynthesis, business, metabolism, Undergraduates
Abstract: We present results of the first large-scale interlaboratory study carried out in synthetic biology, as part of the 2014 and 2015 International Genetically Engineered Machine (iGEM) competitions. Participants at 88 institutions around the world measured fluorescence from three engineered constitutive constructs in E. coli. Few participants were able to measure absolute fluorescence, so data was analyzed in terms of ratios. Precision was strongly related to fluorescent strength, ranging from 1.54-fold standard deviation for the ratio between strong promoters to 5.75-fold for the ratio between the strongest and weakest promoter, and while host strain did not affect expression ratios, choice of instrument did. This result shows that high quantitative precision and reproducibility of results is possible, while at the same time indicating areas needing improved laboratory practices. Copyright: © 2016 Beal et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Published: 2016

50. Mec1, INO80, and the PAF1 complex cooperate to limit transcription replication conflicts through RNAPII removal during replication stress

Author: Alessandro Tosi, Nicole Hustedt, Franz Herzog, Kenji Shimada, Susan M. Gasser, Andrew Seeber, Christian-Benedikt Gerhold, Karl-Peter Hopfner, Ragna Sack, Jérôme Poli, Philippe Pasero, Friedrich Miescher Institute for Biomedical Research, CH-4058 Basel, Switzerland, Gene Center, Ludwig Maximilian University of Munich, 81377 Munich, Germany, Institut de génétique humaine (IGH), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), and Faculty of Natural Sciences, University of Basel, CH-4056 Basel, Switzerland
Subjects: 0301 basic medicine, DNA Replication, Saccharomyces cerevisiae Proteins, Eukaryotic DNA replication, Saccharomyces cerevisiae, Protein Serine-Threonine Kinases, Pre-replication complex, DNA replication factor CDT1, 03 medical and health sciences, Replication factor C, Control of chromosome duplication, Minichromosome maintenance, Stress, Physiological, Gene Expression Regulation, Fungal, Genetics, ComputingMilieux_MISCELLANEOUS, [SDV.GEN]Life Sciences [q-bio]/Genetics, biology, Intracellular Signaling Peptides and Proteins, Nuclear Proteins, 030104 developmental biology, Licensing factor, Mutation, biology.protein, Origin recognition complex, RNA Polymerase II, Developmental Biology, Research Paper
Abstract: Little is known about how cells ensure DNA replication in the face of RNA polymerase II (RNAPII)-mediated transcription, especially under conditions of replicative stress. Here we present genetic and proteomic analyses from budding yeast that uncover links between the DNA replication checkpoint sensor Mec1–Ddc2 (ATR–ATRIP), the chromatin remodeling complex INO80C (INO80 complex), and the transcription complex PAF1C (PAF1 complex). We found that a subset of chromatin-bound RNAPII is degraded in a manner dependent on Mec1, INO80, and PAF1 complexes in cells exposed to hydroxyurea (HU). On HU, Mec1 triggers the efficient removal of PAF1C and RNAPII from transcribed genes near early firing origins. Failure to evict RNAPII correlates inversely with recovery from replication stress: paf1Δ cells, like ino80 and mec1 mutants, fail to restart forks efficiently after stalling. Our data reveal unexpected synergies between INO80C, Mec1, and PAF1C in the maintenance of genome integrity and suggest a mechanism of RNAPII degradation that reduces transcription–replication fork collision.
Published: 2016

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