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ABSTRACT. Superovulasi merupakan suatu metode pemanfaatan induk betina unggul untuk menghasilkan embrio sebanyak-banyaknya untuk kegiatan transfer embrio. Tujuan penelitian ini membandingkan 2 teknik superovulasi yang berbeda pada sapi persilangan Belgian Blue. Penelitian dilakukan di Balai Embrio Ternak Cipelang Bogor dengan menggunakan 24 ekor sapi persilangan Belgian Blue dengan umur 2-3 tahun, masing-masing perlakuan 12 ekor. Penelitian dilakukan secara eksperimen dengan dua perlakuan, P1 = Superovulasi menggunakan penyuntikan FSH selama 3 hari pada pagi dan sore (metode konvensional) dengan dosis 400 mg FSH dalam 20 ml pelarut dan P2 = Superovulasi menggunakan penyuntikan tunggal (satu kali) FSH dengan dosis 400 mg FSH dalam 3 ml pelarut . Parameter pada penelitian ini adalah Jumlah Corpus luteum (CL), respon rate, perolehan embrio, kualitas embrio, recovery rate, dan tingkat fertilisasi. Data yang diperoleh diuji dengan uji T tidak berpasangan. Hasil analisa data menunjukkan hasil yang tidak berbeda nyata (P0,05) pada jumlah CL P1 : 8,42+3,06 dan P2 : 6,08+4,74; Respon rate P1 : 100% dan P2 : 75%; Total perolehan embrio P1 : 6,08+2,64 embrio dan P2 : 4,45+4,01 embrio; Embrio recovery rate P1 : 70,37+9,18% dan P2 : 61,33+12,12%; Embrio Layak Transfer P1 : 3,83+2,92 embrio dan P2 : 2,73+2,28 embrio; dan berbeda nyata (P0,05) pada rataan fertilisasi P1 : 79,10% dan P2 : 95,26%. Kesimpulan penelitian ini adalah teknik superovulasi penyuntikan tunggal FSH secara subkutan memberikan efek superovulasi dan menghasilkan embrio dengan jumlah dan kualitas yang tidak berbeda nyata dengan teknik superovulasi konvensional. (Superovulation Responses of Belgian Blue Crossbreed Cattle Treated with Different Superovulation Methods) ABSTRAK. Superovulation is a technique for producing a large number of embryos for embryo transfer using a genetically superior female. The purpose of this research was to compare two alternative methods of superovulation in Belgian Blue crossbreed cattle. The study used 24 Belgian Blue crossbred cattle aged 2-3 years, including 12 cows per treatment, at the National Livestock Embryo Center of Cipelang in Bogor. The research was done in an experimental setting using two different treatments, P1 = Superovulation using twice daily FSH injections for three days at a dose of 400 mg FSH dissolved in 20 ml of saline, and P2 = Superovulation using FSH single injections at a dose of 400 mg FSH dissolved in 3 ml of saline. The parameters in this study were the number of corpus luteum (CL), response rate, total number of embryo/ova collection, embryo quality, recovery rate, and fertilization rate. The data obtained were tested by unpaired T test. The results of data analysis showed results that were not significantly different (P0.05) on the number of CL P1 : 8.42+3.06 and P2 : 6.08+4.74; Response rate P1 : 100% and P2 : 75%; Total number of embryos collection P1 : 6.08+2.64 embryos and P2 : 4.45+4.01 embryos; Embryo recovery rate P1 : 70.37+9.18% and P2 : 61.33+12.12%; Transferable Embryos P1 : 3.83+2.92 embryos and P2 : 2.73+2.28 embryos; and significantly different (P0.05) on fertilization rate P1 : 79.10% and P2 : 95.26%. The conclusion of this study show that the subcutaneous FSH single injection technique induces superovulation and produces embryos that are similar in number and quality to those produced by conventional superovulation techniques.

The slow-intrinsic-pacemaker dopaminergic (DA) neurons originating in the ventral tegmental area (VTA) are implicated in various mood- and emotion-related disorders, such as anxiety, fear, stress and depression. Abnormal activity of projection-specific VTA DA neurons is the key factor in the development of these disorders. Here, we describe the crucial role of the NALCN and TRPC6, non-selective cation channels in mediating the subthreshold inward depolarizing current and driving the firing of action potentials of VTA DA neurons in physiological conditions. Furthermore, we demonstrate that down-regulation of TRPC6 protein expression in the VTA DA neurons likely contributes to the reduced activity of projection-specific VTA DA neurons in chronic mild unpredictable stress (CMUS) depressive mice. In consistent with these, selective knockdown of TRPC6 channels in the VTA DA neurons conferred mice with depression-like behavior. This current study suggests down-regulation of TRPC6 expression/function is involved in reduced VTA DA neuron firing and chronic stress-induced depression-like behavior of mice.

The relative influence of geography, currents, and environment on gene flow within sessile marine species remains an open question. Detecting subtle genetic differentiation at small scales is challenging in benthic populations due to large effective population sizes, general lack of resolution in genetic markers, and because barriers to dispersal often remain elusive. Marine lakes can circumvent confounding factors by providing discrete and replicated ecosystems. Using high-resolution double digest restriction-site-associated DNA sequencing (4826 Single Nucleotide Polymorphisms, SNPs), we genotyped populations of the sponge Suberites diversicolor (n = 125) to test the relative importance of spatial scales (1-1400 km), local environmental conditions, and permeability of seascape barriers in shaping population genomic structure. With the SNP dataset, we show strong intralineage population structure, even at scales

Software Defined Networking (SDN) adalah arsitektur jaringan baru yang memisahkan antara control dan data plane. Aspek keamanan utama dalam control plane salah satunya adalah serangan DoS dan DDoS. Serangan DDoS mengakibatkan terjadinya penurunan performa jaringan yang berjalan sangat lambat. Serangan DDoS dilakukan dengan menyusupi dan membanjiri bandwidth ke sumber daya target, sehingga dapat menyebabkan penolakan layanan bagi pengguna yang mengaksesnya. Tak hanya itu, serangan DDoS menyebabkan penurunan sumber daya jaringan seperti kapasitas memory dan CPU. Akibatnya kerusakan signifikan pada sistem yang menjadi korban serangan dapat mengalami kerugian, baik secara finansial, reputasi bahkan kehilangan pelanggan yang membutuhkan layanan tersebut. Mencegah serangan DDoS diperlukan suatu tindakan pencegahan yaitu dengan deteksi dini serangan DDoS untuk mengurangi dampak serangan dan memulihkan sistem dengan lebih cepat. Deteksi dini yang disebabkan oleh DDoS pada jaringan SDN dilakukan melalui pendekatan metrik entropy berbasis teori informasi. Penelitian ini memfokuskan pendeteksian dini pada serangan DDoS di dalam lingkungan SDN melalui metode Shannon Entropy dengan mendeteksi lalu lintas atau trafik normal dan DDoS. Penelitian ini menggunakan dataset publik dari InSDN yang diterbitkan pada tahun 2020 untuk menentukan nilai ambang batas lalu lintas normal dan DDoS. Hasilnya, penelitian ini berhasil mendeteksi dini lalu lintas normal dan lalu lintas serangan DDoS dengan nilai entropy sesuai ambang batas, dengan nilai akurasi 100%, presisi 100% dan recall 100% yang dihitung menggunakan confusion matrix. Deteksi dini menampilkan akurasi dan performa yang dapat berkontribusi banyak dalam menunjang tingkat keamanan melalui pencegahan tahap awal, sehingga hasilnya dapat meningkatkan keamanan dan efektifitas pada lingkungan SDN.

The COVID-19 pandemic brought about many challenges to course delivery methods, which have forced institutions to rapidly change and adopt innovative approaches to provide remote instruction as effectively as possible. Creating and preparing content that ensures the success of all students, including those who are deaf and hard-of-hearing has certainly been an all-around challenge. This study aims to investigate the e-learning experiences of deaf students, focusing on the college of the Technical and Vocational Training Corporation (TVTC) in the Kingdom of Saudi Arabia (KSA). Particularly, we study the challenges and concerns faced by deaf students during the sudden shift to online learning. We used a mixed-methods approach by conducting a survey as well as interviews to obtain the information we needed. Our study delivers several important findings. Our results report problems with internet access, inadequate support, inaccessibility of content from learning systems, among other issues. Considering our findings, we argue that institutions should consider a procedure to create more accessible technology that is adaptable during the pandemic to serve individuals with diverse needs.

ObjectiveThis study is a replication and extension of previous work examining the well-being of patients at a breast biopsy appointment. Expanding on a previous study, we aim to identify predictors of well-being following the appointment (i.e. waiting for results).DesignIn this longitudinal study, female patients (N = 197) were surveyed at their breast biopsy appointments and then completed daily surveys assessing distress and coping during the week-long wait for results.Main outcome measuresSurveys asked about patient characteristics, subjective health, cancer history, support availability, outcome expectations, and distress.Results/conclusionsConsistent with the previous study, health history and demographic factors were largely unassociated with distress, this time while waiting for biopsy results. Latina ethnicity emerged one of the few predictors of coping, pointing to opportunities for differential clinical interventions that take cultural factors into account. Finally, anxiety was highest at the beginning and end of the wait for biopsy results, suggesting that interventions may be most effective following a breast biopsy and the days prior to learning one's result.