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1. Dynamic changes between two LHCX-related energy quenching sites control diatom photoacclimation

Author

Marianne Jaubert, Herbert van Amerongen, Jean-Pierre Bouly, Giulio Rocco Stella, Bernard Lepetit, Angela Falciatore, Roberto Bassi, Giovanni Finazzi, Volha U. Chukhutsina, Lucilla Taddei, Biologie Computationnelle et Quantitative = Laboratory of Computational and Quantitative Biology (LCQB), Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratory of Biophysics and MicroSpectroscopy Research Facility, Wageningen University, LaserLaB Vrije Universiteit Amsterdam (LaserLaB), Vrije Universiteit Amsterdam [Amsterdam] (VU), Department of Physics and Astronomy [Amsterdam], Department of Plant Ecophysiology, University of Konstanz, Department of Biotechnology [Verona] (UNIVR | DBT), University of Verona (UNIVR), Physiologie cellulaire et végétale (LPCV), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Marie Curie Initial Training Network Accliphot (FP7-PEPOPLE-2012- ITN, 316427), Marie Curie Initial Training Network CALIPSO (ITN 2013 GA 607607), Human Frontier Science Program (HFSP0052), Zukunftskolleg Konstanz and the Deutsche Forschungsgemeinschaft (DFG-LE 3358/3-1), ANR-12-BIME-0005,DiaDomOil,Domestication des diatomées pour la production de biocarburants(2012), ANR-10-LABX-0049,GRAL,Grenoble Alliance for Integrated Structural Cell Biology(2010), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Department of Biotechnology, University of Verona, Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), ANR-12- BIME-0005,DiaDomOil,DiaDomOil, ANR-10- LABX-49-01,Labex GRAL,Labex GRAL, Biophysics Photosynthesis/Energy, LaserLaB - Energy, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), and Università degli studi di Verona = University of Verona (UNIVR)

Subjects
Chlorophyll, 0106 biological sciences, 0301 basic medicine, Chloroplasts, Light, Photosystem II, Physiology, Acclimatization, Light-Harvesting Protein Complexes, LHCX1, Plant Science, 01 natural sciences, Phaeodactylum tricornutum, Energy quenching, algae, diatoms, photosynthesis, Protein Isoforms, Photosynthesis, algae, chemistry.chemical_classification, biology, Chemistry, Articles, Photochemical Processes, Biofysica, Gene Knockdown Techniques, Light acclimation, Biophysics, Chloroplast, Fluorescence, diatoms, 03 medical and health sciences, Genetics, Life Science, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, SDG 14 - Life Below Water, Marine diatoms, VLAG, Quenching (fluorescence), Organisms, Genetically Modified, fungi, Non photochemical quenching, Photosystem II Protein Complex, biology.organism_classification, 030104 developmental biology, Diatom, Gene Expression Regulation, Xanthophyll, Photoprotection, EPS, 010606 plant biology & botany
Abstract

International audience; Marine diatoms are prominent phytoplankton organisms that perform photosynthesis in extremely variable environments. Diatoms possess a strong ability to dissipate excess absorbed energy as heat via nonphotochemical quenching (NPQ). This process relies on changes in carotenoid pigment composition (xanthophyll cycle) and on specific members of the light-harvesting complex family specialized in photoprotection (LHCXs), which potentially act as NPQ effectors. However, the link between light stress, NPQ, and the existence of different LHCX isoforms is not understood in these organisms. Using picosecond fluorescence analysis, we observed two types of NPQ in the pennate diatom Phaeodactylum tricornutum that were dependent on light conditions. Short exposure of low-light-acclimated cells to high light triggers the onset of energy quenching close to the core of photosystem II, while prolonged light stress activates NPQ in the antenna. Biochemical analysis indicated a link between the changes in the NPQ site/mechanism and the induction of different LHCX isoforms, which accumulate either in the antenna complexes or in the core complex. By comparing the responses of wild-type cells and transgenic lines with a reduced expression of the major LHCX isoform, LHCX1, we conclude that core complex-associated NPQ is more effective in photoprotection than is the antenna complex. Overall, our data clarify the complex molecular scenario of light responses in diatoms and provide a rationale for the existence of a degenerate family of LHCX proteins in these algae.

Published
2018
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2. A systems-wide understanding of photosynthetic acclimation in algae and higher plants

Author

Roberto Bassi, Ioannis Dikaios, Brieuc Urbain, Michel Goldschmidt-Clermont, Oliver Ebenhöh, Anna Matuszyńska, Stephanie Spelberg, Julie Maguire, Kailash Adhikari, Adeline Le Monnier, Dipali Singh, Stefano Magni, Giulio Rocco Stella, Lucilla Taddei, Federica Cariti, Claudia Zabke, Fiona Wanjiku Moejes, Antonella Succurro, Kathrin Müller, Giovanni Finazzi, Mark G. Poolman, Valeria Villanova, Serena Flori, Guillaume Cogne, Angela Falciatore, Cluster of Excellence on Plant Sciences (CEPLAS), Institute of Quantitative and Theoretical Biology, Heinrich Heine Universität Düsseldorf = Heinrich Heine University [Düsseldorf], Bantry Marine Research Station, Department of Biological and Medical Sciences, Oxford Brookes University, Department of Biotechnology, University of Tehran-University College of Science, Department of Botany and Plant Biology, University of Geneva, Laboratoire de génie des procédés - environnement - agroalimentaire (GEPEA), Institut Universitaire de Technologie - Nantes (IUT Nantes), Université de Nantes (UN)-Université de Nantes (UN)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Institut Universitaire de Technologie Saint-Nazaire (IUT Saint-Nazaire), Université de Nantes (UN)-Ecole Polytechnique de l'Université de Nantes (EPUN), Université de Nantes (UN)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Centre National de la Recherche Scientifique (CNRS)-Université Bretagne Loire (UBL)-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Institut Universitaire de Technologie - La Roche-sur-Yon (IUT La Roche-sur-Yon), Université de Nantes (UN), Centre National de la Recherche Scientifique (CNRS), Ecole Nationale Vétérinaire Agroalimentaire et de l'Alimentation Nantes Atlantique (ONIRIS), PRES Université Nantes Angers Le Mans (UNAM), Biologie Computationnelle et Quantitative = Laboratory of Computational and Quantitative Biology (LCQB), Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC), Sorbonne Université (SU), UMR 1417 PCV Laboratoire de Physiologie Cellulaire Végétale., Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut National de la Recherche Agronomique (INRA), Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Fermentalg SA, IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT), University of Aberdeen, Marie Curie Initial Training Network project, AccliPhot [PITN-GA-2012-316427], Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS)-Ecole Polytechnique de l'Université de Nantes (EPUN), Université de Nantes (UN)-Université de Nantes (UN)-Institut Universitaire de Technologie - Nantes (IUT Nantes), Université de Nantes (UN)-Institut Universitaire de Technologie Saint-Nazaire (IUT Saint-Nazaire), Université de Nantes (UN)-Institut Universitaire de Technologie - La Roche-sur-Yon (IUT La Roche-sur-Yon), Université de Nantes (UN)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Université Bretagne Loire (UBL), Heinrich-Heine-Universität Düsseldorf [Düsseldorf], Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie (Paris 6), Sorbonne Universités, Université Grenoble Alpes (UGA), Ecole Nationale Supérieure des Mines de Nantes (Mines Nantes), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Université de Nantes (UN)-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Centre National de la Recherche Scientifique (CNRS)-Université Bretagne Loire (UBL)-IMT Atlantique (IMT Atlantique), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), IMT Atlantique (IMT Atlantique), Moejes F.W., Matuszynska A., Adhikari K., Bassi R., Cariti F., Cogne G., Dikaios I., Falciatore A., Finazzi G., Flori S., Goldschmidt-Clermont M., Magni S., Maguire J., Le Monnier A., Muller K., Poolman M., Singh D., Spelberg S., Stella G.R., Succurro A., Taddei L., Urbain B., Villanova V., Zabke C., and Ebenhoh O.

Subjects
0301 basic medicine, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Physiology, Acclimatization, Context (language use), PhD training, interdisciplinary training, Plant Science, Biochemistry, biophysics & molecular biology [F05] [Life sciences], Biology, acclimation, Photosynthesis, Models, Biological, modelling, 03 medical and health sciences, Algae, Chlorophyta, application industrielle, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, mathematical modelling, Biochimie, biophysique & biologie moléculaire [F05] [Sciences du vivant], biodiversity, modélisation, micro-algue, Phototroph, photosynthetic system, Ecology, Non-photochemical quenching, Systems Biology, acclimatation photosynthétique, photosynthetic optimisation, Plankton, Plants, analyse rétrospective, biology.organism_classification, industrial application, European Training Network, 030104 developmental biology, Acclimation, microalgal cultivation, non-photochemical quenching, Photosynthetic acclimation, adaptation à la lumière, appareil photosynthétique, Biochemical engineering
Abstract

The ability of phototrophs to colonise different environments relies on robust protection against oxidative stress, a critical requirement for the successful evolutionary transition from water to land. Photosynthetic organisms have developed numerous strategies to adapt their photosynthetic apparatus to changing light conditions in order to optimise their photosynthetic yield, which is crucial for life on Earth to exist. Photosynthetic acclimation is an excellent example of the complexity of biological systems, where highly diverse processes, ranging from electron excitation over protein protonation to enzymatic processes coupling ion gradients with biosynthetic activity, interact on drastically different timescales from picoseconds to hours. Efficient functioning of the photosynthetic apparatus and its protection is paramount for efficient downstream processes, including metabolism and growth. Modern experimental techniques can be successfully integrated with theoretical and mathematical models to promote our understanding of underlying mechanisms and principles. This review aims to provide a retrospective analysis of multidisciplinary photosynthetic acclimation research carried out by members of the Marie Curie Initial Training Project, AccliPhot, placing the results in a wider context. The review also highlights the applicability of photosynthetic organisms for industry, particularly with regards to the cultivation of microalgae. It intends to demonstrate how theoretical concepts can successfully complement experimental studies broadening our knowledge of common principles in acclimation processes in photosynthetic organisms, as well as in the field of applied microalgal biotechnology.

Published
2017
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3. Hosting the Unwanted: Stethoscope Contamination Threat

Author

Gabriele Messina, Lucilla Taddei, L. Mariani, Nicola Nante, D Lenzi, Sandra Burgassi, Carmela Russo, Emma Ceriale, and P Manzi

Subjects
Environmental Engineering, Meticillin, biology, Stethoscope, medicine.drug_class, business.industry, Microorganism, Antibiotics, Drug resistance, Contamination, biology.organism_classification, Industrial and Manufacturing Engineering, Coliform bacteria, law.invention, Microbiology, law, medicine, business, Bacteria, medicine.drug
Abstract

Aims: Stethoscopes represent a vehicle of bacteria and other microorganisms and may play a role in the spread of health -care associated infections (HAIs). We aimed to evaluate the contamination levels of

Published
2014

4. The DivJ, CbrA and PleC system controls DivK phosphorylation and symbiosis inSinorhizobium meliloti

Author

Alessio Mengoni, Antonella Fioravanti, Saswat S. Mohapatra, Emanuele G. Biondi, Nicole J. De Nisco, Anke Becker, Matteo Brilli, Lucilla Taddei, Marco Bazzicalupo, Frédérique Dewitte, Graham C. Walker, Vincent Villeret, Francesco Pini, Benjamin Frage, Marco Galardini, and Lorenzo Ferri

Subjects
0303 health sciences, Sinorhizobium meliloti, biology, Membrane permeability, 030306 microbiology, Caulobacter crescentus, Kinase, Histidine kinase, food and beverages, Cell cycle, biology.organism_classification, Microbiology, 03 medical and health sciences, Response regulator, Biochemistry, bacteria, Phosphorylation, Molecular Biology, 030304 developmental biology
Abstract

Sinorhizobium meliloti is a soil bacterium that invades the root nodules it induces on Medicago sativa, whereupon it undergoes an alteration of its cell cycle and differentiates into nitrogen-fixing, elongated and polyploid bacteroid with higher membrane permeability. In Caulobacter crescentus, a related alphaproteobacterium, the principal cell cycle regulator, CtrA, is inhibited by the phosphorylated response regulator DivK. The phosphorylation of DivK depends on the histidine kinase DivJ, while PleC is the principal phosphatase for DivK. Despite the importance of the DivJ in C. crescentus, the mechanistic role of this kinase has never been elucidated in other Alphaproteobacteria. We show here that the histidine kinases DivJ together with CbrA and PleC participate in a complex phosphorylation system of the essential response regulator DivK in S. meliloti. In particular, DivJ and CbrA are involved in DivK phosphorylation and in turn CtrA inactivation, thereby controlling correct cell cycle progression and the integrity of the cell envelope. In contrast, the essential PleC presumably acts as a phosphatase of DivK. Interestingly, we found that a DivJ mutant is able to elicit nodules and enter plant cells, but fails to establish an effective symbiosis suggesting that proper envelope and/or low CtrA levels are required for symbiosis.

Published
2013
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5. Impact of a disinfecting technique on microbial contamination of computer keyboards and telephone handsets

Author

L. Mariani, Sandra Burgassi, Lucilla Taddei, Chiara Defranceschi, Gabriele Messina, D Lenzi, Emma Ceriale, P Manzi, and Carmela Russo

Subjects
Toxicology, Wilcoxon signed-rank test, business.industry, Microbial contamination, Contamination, Total bacterial count, Telecommunications, business, Pathogenic microorganism, Mathematics
Abstract

Background: Computers and telephones are ubiquitous in the hospital and have been shown to be contaminated with potentially pathogenic microorganism. The aim of the study was to determine microbial contamination on computer keyboards and telephone handsets and the effectiveness of a disinfecting technique (DT). Methods: A matched cross-over study, involving an overall of 50 computer keyboards and 50 telephone handsets, was conducted in three hospitals, located in the Local Health Area of Siena (Italy) before and after the use of an innovative DT consisting of a malleable-elastic compound, containing ethanol, which adheres to surfaces, removes dirt and disinfects. Total bacterial count was evaluated and several types of bacteria and fungi were researched pre- and post- use of the DT. Non parametric tests: Wilcoxon signed rank and Mann Whitney, were used, when appropriate. Results: The DT was effective in disinfecting the objects. In fact, Colony-Forming Units (CFUs) decreased to zero in most comparisons. All the comparisons showed significant differences ( p

Published
2013
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